CN109221606A - A kind of nonreactive shrimp crab mulberry leaf liquid feed additive and the preparation method and application thereof - Google Patents

A kind of nonreactive shrimp crab mulberry leaf liquid feed additive and the preparation method and application thereof Download PDF

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Publication number
CN109221606A
CN109221606A CN201811063859.1A CN201811063859A CN109221606A CN 109221606 A CN109221606 A CN 109221606A CN 201811063859 A CN201811063859 A CN 201811063859A CN 109221606 A CN109221606 A CN 109221606A
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China
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mulberry leaf
slurries
shrimp crab
nonreactive
liquid feed
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CN201811063859.1A
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Inventor
李飞鸣
罗安乐
黄仁志
张伟军
李霞
龙唐忠
叶添梅
徐清波
李双庆
彭大坪
刘根
李平
李一平
颜新培
艾均文
宋南平
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SERICULTURAL RESEARCH INSTITUTE OF HUNAN PROVINCE
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SERICULTURAL RESEARCH INSTITUTE OF HUNAN PROVINCE
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Priority to CN201811063859.1A priority Critical patent/CN109221606A/en
Publication of CN109221606A publication Critical patent/CN109221606A/en
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/80Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass

Abstract

The present invention provides a kind of nonreactive shrimp crab mulberry leaf liquid feed additive and the preparation method and application thereof, and the nonreactive shrimp crab mulberry leaf liquid feed additive includes the component of following mass percent: new fresh mulberry leaf slurries 60~70%;Fresh feeding fly maggot slurries 25~35%;Brown sugar water 4.5~5.5%;Mixed bacteria 0.01~0.03%.Preparation method is slurries will to be made in new fresh mulberry leaf, fresh feeding fly maggot mashing, sieving;Brown sugar is tuned into brown sugar water;Then it mixes in proportion and ferments to obtain the final product.The nonreactive shrimp crab mulberry leaf liquid feed additive is adjustable shrimp crab intestine microenvironment, improves shrimp crab to the digestibility of feed, enhances the disease resistance of shrimp crab;It is also adjustable and purifies water simultaneously, reduce shrimp crab lesion and death caused by water pollution, thus the comprehensive quality and yield for improving shrimp crab.

Description

A kind of nonreactive shrimp crab mulberry leaf liquid feed additive and the preparation method and application thereof
Technical field
The present invention relates to a kind of feed for shrimp and crab additive and the preparation method and application thereof more particularly to a kind of nonreactive shrimp crab mulberries Leaf liquid feed additive and the preparation method and application thereof belongs to shrimp crab cultural technique field.
Background technique
Nonreactive cultivation is the abbreviation of antibiotic-free cultivation, refers to and does not have to any kind of antibiotic in breeding process, refers in particular to people It need not with antibiotic and beast birds antibiotic.Moore etc. (1946) is reported for the first time, and antibiotic is added in feed, can be obvious Improve the daily gain of broiler chicken.Since then, people are more and more extensive to the research and application of antibiotic.At the bottom of nineteen fifty, U.S. FDA is formal Approval uses antibiotic in feed, and is gradually applied by countries in the world.Over more than 60 years, antibiotic animal diseases control, Improve efficiency of feed utilization, promote growth of animals or poultry etc. has played important function, successively has more than 60 antibiotic to be applied to herding Aquaculture.
Due to applying antibiotic that there is apparent economic benefit, in addition current high density large-scale cultivation situation in cultivation Lower animal health condition is poor, and aquaculture is caused more to rely on antibiotic application.In aquaculture industry of China, practitioner is commonly plain In the lower situation of matter, the volume that transfinites, the abuse of antibiotics phenomenon of super kind are generally existing.Antibiotic residue in cultured product is caused to refer to Mark is higher, seriously threatens food safety.
The abuse of antibiotic in aquaculture, increases the difficulty of animal diseases control, more directly or indirectly compromises people The health of class.Such as the residual of animal livestock products drug makes mankind pathogen generate drug resistance, generates " superbug ".Most Recent studies on shows that animal husbandry abuse of antibiotics is the source of zoonosis.In Holland, the mankind " superbug " (MRSA, anti-methoxy XiLin staphylococcus aureus or anti-oxacillin staphylococcus aureus) in cases of infection 20% infection initially only go out from a kind of The intracorporal new virus of present pig.The research for being published in " American Medical Association academic periodical " thinks that the whole America in 2005 has l0 ten thousand " super Grade germ " case, wherein have 1.9 ten thousand it is lethal.
In recent years, countries in the world recognize that abuse of antibiotics is led to the problem of, and having come into vogue in developed country does not make With the cultural method of antibiotic, i.e. nonreactive cultivation method, the developing direction of healthy aquaculture is represented.For example, Sweden is early in 1986 Year steps the first step, announces that completely forbidding antibiotic is used as feed addictive.Denmark also prohibits Multiple Classes of Antibiotics conduct successively Growth promoter uses, until 2008, the usage amount of antibiotic is than reducing nearly 50% in the Denmark's country's pig production when highest. 2006, EU member country stopped using all antibiotic growth promoters comprehensively.
The nonreactive of China cultivates i.e. someone's research since 2000, passes through the technologies such as lactic acid bacteria technique, herbal medicine technology Application, a small range realize nonreactive cultivation, in recent years, report is seen in using the nonreactive feed of compound micro-ecological preparation, also There is certain using effect, but due to technology maturation, culture efficiency etc., non-large-scale promotion.But based on environmental Kuznets Curves Nonreactive cultivation has been carried out large-scale farming and achieves excellent achievement.
Currently, China shrimp crab raiser is to pursue culture efficiency, under intensive high-density breeding mode, there is also excessive The phenomenon that drug dose cultivates not only is caused water pollution, but also is severely exceeded in vivo because harmful element is enriched with, and influences to consume The health of person, bait utilization is lower in breeding process, a large amount of residual bait, biological metabolite, animals and plants corpse in breeding process In aquaculture pond bottom, these organic matters corrupt can be decomposed to generate and largely be supported to aquatic products equal organic matter accumulations under the action of anaerobic bacteria The toxic substance of animal is grown, the physicochemical environment and ecological environment severe exacerbation of breeding water body are caused.Shrimp crab cultivation water requires height, The use for how substituting or reducing antibiotic and drug in shrimp crab breeding process, the utilization rate for improving bait is that we need to solve The problem of.
Also there is the relevant report much about nonreactive shrimp crab cultivation additive in existing report:
Documents 1:CN103704531A discloses a kind of calcium supplement for aquaculture, counts by ratio of weight and the number of copies, packet Include following components: 25~33 parts of calcium lactate, 15~30 parts of calcium gluconate, 20~30 parts of marine bioactivity calcium, natural to encourage 15~20 parts of agent, 3~5 parts of magnesium chloride, 3~5 parts of potassium chloride, Vitamin D5~8 part, 6~8 parts of vitamin B6;Wherein, the day Right growth regulator is counted by ratio of weight and the number of copies, including following components: 15~20 parts of the wall fern, 10~15 parts of eclipta, and mulberry leaf 10~15 Part, 10~15 parts of blackspiked lovegrass herb with root, 15~20 parts of achyranthes aspera, 10~15 parts of folium isatidis, 10~15 parts of cassia bark, 10~15 parts of Radix Polygalae, sea 10~15 parts of algae sugar.
Documents 2:CN106798192A discloses a kind of Chinese herbal feed additive for preventing shrimp crab crust canker, institute Stating additive includes following raw material components: fly maggot powder, purslane, pawpaw, dandelion, sponge gourd, dried orange peel, lily, mushroom, peppermint, Kiwi berry, three isooctyl acid glyceride, ferric citrate, Radix Glycyrrhizae, Mel Jujubae, rape pollen and vinegar egg juice;The parts by weight of each raw material Number are as follows: 0.5~1.0 part of fly maggot powder, 4~5 parts of purslane, 10~19 parts of pawpaw, 12~22 parts of dandelion, 4~11 parts of sponge gourd, old 15~28 parts of skin, 8~14 parts of lily, 5~16 parts of mushroom, 9~19 parts of peppermint, 3~14 parts of Kiwi berry, three isooctyl acid glyceride 0.001~0.005 part, 0.001~0.003 part of ferric citrate, 4~12 parts of Radix Glycyrrhizae, 17~31 parts of Mel Jujubae, rape pollen 10 ~23 parts and 7~14 parts of vinegar egg juice.
It is a kind of calcium supplement of aquaculture disclosed in above-mentioned documents 1, wherein contains phase in the component of growth regulator The mulberry leaf of equivalent;In shrimp crab Chinese herbal feed additive disclosed in documents 2, contain the fly more a small amount of compared to other raw materials Maggot powder, the feed addictive are used to improve the disease resistance of shrimp crab and promote growth.
The present invention intends providing a kind of using mulberry leaf and feeding maggot as the not antibiotic nonreactive shrimp crab mulberry leaf of primary raw material Liquid feed additive and preparation method thereof.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of nonreactive shrimp crab mulberry leaf liquid feed additive and its preparation sides Method.
In order to solve the above-mentioned technical problem, the invention adopts the following technical scheme:
Firstly, a kind of nonreactive shrimp crab mulberry leaf liquid feed additive is provided, the component including following mass percent:
New fresh mulberry leaf slurries 60~70%;
Fresh feeding fly maggot slurries 25~35%;
Brown sugar water 4.5~5.5%;
Mixed bacteria 0.01~0.03%.
Further,
The nonreactive shrimp crab mulberry leaf liquid feed additive, the component including following mass percent:
New fresh mulberry leaf slurries 65%, fresh feeding fly maggot slurries 30%, brown sugar water 4.98%, mixed bacteria 0.02%.
Further,
The nonreactive shrimp crab mulberry leaf liquid feed additive, the component including following mass percent:
New fresh mulberry leaf slurries 62.5%, fresh feeding fly maggot slurries 32.5%, brown sugar water 4.97%, mixed bacteria 0.03%.
Further,
The mixed bacteria be saccharomycete, lactic acid bacteria, bacillus subtilis, denitrifying bacteria, at least three kinds in photosynthetic bacteria Mixing.
Further,
The fly maggot is fresh no-sundries fly maggot live larva.
The present invention also provides the preparation methods of above-mentioned nonreactive shrimp crab mulberry leaf liquid feed additive, specifically include following step It is rapid:
S1, feedstock processing
New fresh mulberry leaf is cleaned, is drained, is beaten, is sieved that it is spare to obtain new fresh mulberry leaf slurries;
Fresh feeding fly maggot is also beaten, is sieved that it is spare to obtain feeding fly maggot slurries;
The brown sugar water for being tuned into 50~60% by brown sugar and water is spare;
S2, by obtained new fresh mulberry leaf slurries, feeding fly maggot slurries, brown sugar water and mixed bacteria after step S1 processing according to Ratio is put into constant temperature agitator tank reaction kettle, controls 25~35 DEG C of reaction temperature, while being that 25~40r/min is stirred with mixing speed 8~12min of reaction is mixed, mixed liquor is obtained;The quality hundred of new fresh mulberry leaf slurries, feeding fly maggot slurries, brown sugar water and mixed bacteria Divide ratio as follows:
New fresh mulberry leaf slurries 60~70%;
Fresh feeding fly maggot slurries 25~35%;
Brown sugar water 4.5~5.5%;
Mixed bacteria 0.01~0.03%;
S3, by obtained mixed liquor barrelling after after step S2 reaction, and after being sealed by fermentation to obtain the final product;If fermentation temperature is T, then: when T < 15 DEG C, need to ferment 12~20 days;When 15 <≤T≤30 DEG C, need to ferment 8~12 days;Work as T > 30 DEG C, it need to ferment 5~ 8d。
Above-mentioned fermentation temperature and fermentation time are adjusted with specific reference to environment temperature, and summer temperature is high, fermentation time compared with It is short;Winter temperature is low, and fermentation time is longer.
Further,
In step S1, slurries are obtained by 60~100 meshes respectively after new fresh mulberry leaf, fresh feeding fly maggot mashing.
Further,
The mixed bacteria be saccharomycete, lactic acid bacteria, bacillus subtilis, denitrifying bacteria, at least three kinds in photosynthetic bacteria Mixing.
The present invention also provides above-mentioned nonreactive shrimp crab mulberry leaf liquid feed additives to prepare the application in feed for shrimp and crab, the nothing Anti- shrimp crab mulberry leaf liquid feed additive is adjustable shrimp crab intestine microenvironment, improves shrimp crab to the digestibility of feed, Enhance the disease resistance of shrimp crab;It is also adjustable and purifies water simultaneously, reduce shrimp crab lesion and death caused by water pollution, from And integrate the quality and yield for improving shrimp crab.
Further,
The additive amount of the nonreactive shrimp crab mulberry leaf liquid feed additive is 50~100g/kg feed.
Mechanism of the invention and the utility model has the advantages that
1, mulberry leaf nutrient material abundance is confirmed as the leaves of plants of " integration of drinking and medicinal herbs " for 1993 by health ministry, wherein slightly Protein content 20~36%, and must containing multivitamin and minerals, amino acid, carbohydrate and plant fiber, animal The amino acid content needed is also high, is one of animal and fowl fodder low cost protein raw material.In addition, containing flavonoid in mulberry leaf There is the natural active matters such as object, polysaccharide, sitosterol, isoquercitrin, gamma-amino acid, 1-DNJ resisting stress to make With body resistance against diseases can be enhanced.
2, feeding fly maggot is full of nutrition, and gross protein value is up to 56~63%, containing a variety of necessary to animal body Amino acid and fatty acid, and contain a large amount of vitamins, microelement and antibacterial active peptide, it is a kind of good protein sources.Make Trophic level for high-protein feed, fly maggot can match in excellence or beauty with fish meal, not only can directly watch feed the pig of conventional variety, chicken, duck, Fish, but also be the best living baits such as special animals shrimp, crab, eel, swamp eel, frog, bullfrog, Lampetra mori, spot fish, tortoise.
3, the mixed bacteria in the present invention is by saccharomycete, lactic acid bacteria, bacillus subtilis, denitrifying bacteria, photosynthetic bacteria In the compound probiotic that is combined of a variety of bacterium, these strains increase oxygen by absorbing the oxygen consumption factor in water body indirectly, It is the high-quality bait of zooplankter, zooplankter growth is more vigorous, provides bait for shrimp crab.Mixed Microbes are applied to aquaculture When, the digestibility of enteron aisle can be improved, and regulating water quality can be played the role of, it have have no toxic side effect, have no drug resistance, The features such as noresidue, low cost, significant effect, efficiently against chemical farmings such as antibiotic, hormone, preservative, pesticide, chemical fertilizer Generated various side effects.
4, the brown sugar in feed addictive of the present invention, raw material are sugarcanes, and the ingredient of 95% or more brown sugar is sucrose, in addition Also contain vitamin and microelement, sufficient nutrient can be provided for the breeding of mixed bacteria, promote a large amount of of mixed bacteria Breeding.
5, shrimp crab category omnivorousness shrimps, with planktonic organism, aquatic insect, green alga and water plant etc. for staple food.It is propagating artificially Under the conditions of, it is edible to various fish baits (rice bran, raw bran, soya-bean cake, freshwater mussel meat, spiral shell meat and the flesh of fish) at shrimp, it is fond of vinasse especially Equal fermented feeds, by testing under intensive high-density breeding mode, it was demonstrated that in conjunction with antibacterial in the herbal pharmacologies of mulberry leaf, maggot Active peptide and compound probiotic can reduce or the use of alternative medicine and antibiotic, and effective regulating water quality, improves shrimp crab product Matter and attenuating aquaculture cost.
6, the characteristics of present invention can be rich in antibacterial active peptide by the medicinal characteristic and fly maggot of mulberry leaf improves the anti-of shrimp crab Sick ability reduces the use of drug and antibiotic, adjusts shrimp crab intestine microenvironment and aquatic environment by microbial technique, Feed digestion absorptivity and purifying aquaculture environment are improved, it is comprehensive to improve shrimp crab product quality and aquaculture cost.
7, the present invention organically combines both mulberry leaf vegetable protein and maggot insect protein, and passes through microorganism Polypeptide macromolecule breaks down proteins at the small peptide protein molecule for being easier to absorb, are more advantageous to short by the processing of (mixed bacteria) The nutrient absorption of enteron aisle shrimp crab improves the speed of growth.And the albumen after degrading, the Partial Fish Meal in feed for shrimp and crab can be substituted, Aquaculture cost is effectively reduced.The antibacterial active peptide that fly maggot is rich in is the extraneous cause of disease body-sensing of a kind of resistance that Immune System generates The peptides bioactive substance of dye, mulberry leaf can enhance body resistance against diseases, can substitute or reduce the use of antibiotic, improve shrimp Crab quality.
8, the present invention has Tiny ecosystem water transfer effect, and product generates a large amount of prebiotic strain, mixed bacteria after everfermentation In the withered grass gemma that contains, denitrifying bacteria kind can play the role of adjusting and purify water, feed for shrimp and crab residue can be passed through Microorganism carries out degradation treatment, during avoiding conventional feed from feeding, food-residue and cause shrimp crab caused by water pollution sick Become and dead.
9, by fermentation, saccharomycete, lactic acid bacteria, bacillus subtilis, denitrifying bacteria in fermentation process in mixed liquor, Photosynthetic bacteria meeting mass propagation, so that the product obtained after fermentation can improve rich in probiotics such as a large amount of saccharomycete, lactic acid bacterias Shrimp crab intestinal colony environment improves the digestibility of feed, reduces feeding cost.
10, from preparation method for fresh living body maggot and new fresh mulberry leaf do not need to dry, pass through microbial technique It is saved, processing cost and nutrition loss can be reduced to greatest extent;And be beaten new fresh mulberry leaf, fresh feeding fly maggot, Sieving can allow it sufficiently thorough in fermentation stage fermentation.
11, the present invention, which is able to achieve, is applied to China's mulberry leaf resource in high added value specific feed, improves the additional of mulberry leaf Value.
Specific embodiment
Invention is further illustrated below with reference to embodiment, but is not intended to limit the scope of the invention.
Embodiment 1
The present embodiment provides a kind of preparation methods of nonreactive shrimp crab mulberry leaf liquid feed additive, specifically include following step It is rapid:
S1, feedstock processing
New fresh mulberry leaf is cleaned, is drained, is beaten, crossing 80 meshes, to obtain new fresh mulberry leaf slurries spare;
Fresh feeding fly maggot is also beaten, crossing 80 meshes, to obtain feeding fly maggot slurries spare;
The brown sugar water for being tuned into 60% by brown sugar and water is spare;
S2, according to the new fresh mulberry leaf slurries 65% of mass percent;Fresh feeding fly maggot slurries 30%;Brown sugar water 4.98%; Mixed bacteria 0.02% takes new fresh mulberry leaf slurries 6.5kg made from step S1, fresh feeding fly maggot slurries 3kg, brown sugar water respectively 0.498kg, mixed bacteria 0.002kg, wherein mixed bacteria be saccharomycete, lactic acid bacteria, bacillus subtilis, denitrifying bacteria, The mixture of five kinds of bacterium of photosynthetic bacteria, saccharomycete: lactic acid bacteria: bacillus subtilis: denitrifying bacteria: the mass ratio of photosynthetic bacteria is 1: 1:1:1:1.Then material is put into constant temperature agitator tank reaction kettle, controls 25~35 DEG C of reaction temperature, while with mixing speed It is stirred to react 8~12min for 25~40r/min, obtains mixed liquor;
S3, will obtained mixed liquor after step S2 reaction, using measuring packing machine barrelling, and after being sealed by fermentation, hair It ferments 8~12 days under the conditions of 15~30 DEG C of ferment temperature;To obtain the final product, it is denoted as sample 1.
Embodiment 2
The present embodiment the difference from embodiment 1 is that, step S2, according to the new fresh mulberry leaf slurries 62.5% of mass percent, Fresh feeding fly maggot slurries 32.5%, brown sugar water 4.97%, mixed bacteria 0.03% take new fresh mulberry leaf made from step S1 respectively Slurries 6.25kg, fresh feeding fly maggot slurries 3.25kg, brown sugar water 0.497kg, mixed bacteria 0.003kg, other steps and reality It is identical to apply example 1, sample obtained is denoted as sample 2.
Embodiment 3
The present embodiment the difference from embodiment 1 is that, step S2, according to the new fresh mulberry leaf slurries 60% of mass percent, newly Fresh feeding fly maggot slurries 34.49%, brown sugar water 5.5%, mixed bacteria 0.01% take new fresh mulberry leaf slurry made from step S1 respectively Liquid 6kg, fresh feeding fly maggot slurries 3.449kg, brown sugar water 0.55kg, mixed bacteria 0.001kg, other steps and embodiment 1 Identical, sample obtained is denoted as sample 3.
Embodiment 4
The present embodiment the difference from embodiment 1 is that, step S2, according to the new fresh mulberry leaf slurries 70% of mass percent, newly Fresh feeding fly maggot slurries 25.47%, brown sugar water 4.5%, mixed bacteria 0.03% take new fresh mulberry leaf slurry made from step S1 respectively Liquid 7kg, fresh feeding fly maggot slurries 2.547kg, brown sugar water 0.45kg, mixed bacteria 0.003kg, other steps and embodiment 1 Identical, sample obtained is denoted as sample 4.
Embodiment 5
The present embodiment the difference from embodiment 1 is that, step S2, according to the new fresh mulberry leaf slurries 70% of mass percent, newly Fresh feeding fly maggot slurries 25%, brown sugar water 4.98%, mixed bacteria 0.02% take new fresh mulberry leaf slurries made from step S1 respectively 7kg, fresh feeding fly maggot slurries 2.5kg, brown sugar water 0.498kg, mixed bacteria 0.002kg, other steps and 1 phase of embodiment Together, sample obtained is denoted as sample 5.
Embodiment 6
The present embodiment the difference from embodiment 1 is that, in step S2 mixed bacteria use saccharomycete, lactic acid bacteria, withered grass bud The mixture of four kinds of spore bacillus, denitrifying bacteria bacterium, saccharomycete: lactic acid bacteria: bacillus subtilis: the mass ratio of denitrifying bacteria For 1:1:1:1.Other steps are same as Example 1, and sample obtained is denoted as sample 6.
Embodiment 7
The present embodiment the difference from embodiment 1 is that, in step S2 mixed bacteria use saccharomycete, lactic acid bacteria, denitrification The mixture of three kinds of bacterium of bacterium, saccharomycete: lactic acid bacteria: the mass ratio of denitrifying bacteria is 1:1:1.Other steps and embodiment 1 Identical, sample obtained is denoted as sample 7.
Embodiment 8
The present embodiment the difference from embodiment 1 is that, in step S2 mixed bacteria use saccharomycete, lactic acid bacteria, withered grass bud The mixture of three kinds of bacterium of spore bacillus, saccharomycete: lactic acid bacteria: the mass ratio of bacillus subtilis is 1:1:1.Other steps and implementation Example 1 is identical, and sample obtained is denoted as sample 8.
Embodiment 9
The present embodiment the difference from embodiment 1 is that, in step S2 mixed bacteria use saccharomycete, lactic acid bacteria, photosynthetic bacteria The mixture of three kinds of bacterium, saccharomycete: lactic acid bacteria: the mass ratio of photosynthetic bacteria is 1:1:1.Other steps are same as Example 1, are made Sample be denoted as sample 9.
Comparative example 1
The present embodiment the difference from embodiment 1 is that, according to the new fresh mulberry leaf slurries 65% of mass percent in step S2;Newly Fresh feeding fly maggot slurries 30%;Brown sugar water 5% takes new fresh mulberry leaf slurries 6.5kg, fresh feeding fly maggot made from step S1 respectively Slurries 3kg, brown sugar water 0.5kg, are added without mixed bacteria.Other steps are same as Example 1, and sample obtained is denoted as sample 10。
Comparative example 2
The present embodiment the difference from embodiment 1 is that, according to the new fresh mulberry leaf slurries 95% of mass percent in step S2;It is red Syrup 4.98%;Mixed bacteria 0.02%, take respectively new fresh mulberry leaf slurries 9.5kg made from step S1, brown sugar water 0.498kg, Mixed bacteria 0.002kg.Other steps are same as Example 1, and sample obtained is denoted as sample 11.
Test is carried out to above-mentioned sample and compares analysis
(1) test material: the shrimp seedling 6kg of height about 3cm is chosen;
(2) test method: above-mentioned shrimp seedling 6kg is randomly divided into 12 groups, every group of 500g, subregion raising, wherein 11 groups are real Group is tested, the feed for shrimp and crab of feeding addition 1~sample of sample, 11 feed addictive is (according to the sample of every kg feed addition 100g respectively 1~11), remaining 1 group is blank control group, and blank control group is the feed for shrimp and crab of feeding phase homogenous quantities.
The composition of each group feed addictive is as shown in table 1 below:
The composition of 1 each group feed addition of table
(3) it is raised respectively according to the same terms subregion, observes the growing state of water quality situation and shrimp, feed January, 2 After month, shrimp 25 are arbitrarily selected from each area respectively, the single height and quality only of measurement simultaneously records, as a result such as the following table 2:
It was found from above-mentioned 1 result:
1, the shrimp seedling of Examples 1 to 9 sample feed addictive feeding, head and substance all compare comparative example sample 10, 11 want it is excellent very much, show that nonreactive feed for shrimp and crab additive provided in an embodiment of the present invention can promote the growth of shrimp crab, and feed The enteron aisle of shrimp crab out is transparent, illustrates that shrimp crab is high for the digestibility of feed, and survival rate is high during nursing, no It needs to add antibiotic usage, can reduce cultivation finished product.
2, the feeding effect of sample 1~9 is especially best with the effect of sample 1 made from embodiment, illustrates feed addictive In new fresh mulberry leaf slurries: fresh feeding fly maggot slurries: brown sugar water: the ratio of mixed bacteria is controlled in 65:30:4.98:0.02, and When using saccharomycete, lactic acid bacteria, bacillus subtilis, five kinds of denitrifying bacteria, photosynthetic bacteria bacterium simultaneously, feeding effect is best.It is real Example 2~5 is applied, the ratio of each component of the feed addictive is adjusted, effect is slightly worse than embodiment 1.Embodiment 6~9 is reduced The type of strain simultaneously adjusts type, illustrates that reducing for species still can be in the limpid degree of water quality and water Oxygen content affects, and can specifically reduce the oxygen content in the limpid degree and water of water quality, and can be to a certain extent Influence the survival rate and disease resistance of shrimp.And from the point of view of the result of the sample of comparative example 1 10 (not adding mixed bacteria), water Matter can become muddy, and oxygen content sharply declines, and enteron aisle blacks, and illustrates that the digestibility of shrimp receives large effect, Feed is easy accumulation in shrimp body and blacks, and water quality cannot adjust improvement, to become muddy.The sample 11 of comparative example 2 From the point of view of the result of (not adding fresh feeding fly maggot slurries), water quality impact is little, but fresh feeding fly is relatively added in the speed of growth The embodiment 1-9 of maggot slurries is many slowly, and the head and substance of shrimp have obvious decline, illustrates that growth is more slow, And the resistance of shrimp also reduces, survival rate receives influence, needs to add antibiotic to increase the survival rate of shrimp.
3, to launch 5w shrimp seedling calculating per acre, about 50kg can be increased production per acre.
The fermentation time that the present invention prepares the nonreactive feed for shrimp and crab additive is adjusted accordingly according to seasonal temperature, if hair Ferment temperature < 15 DEG C need to ferment 12~20 days;Fermentation temperature T > 30 DEG C need to only ferment 5~8d, when the sample preparation of the present embodiment Between at the beginning of 4 months, temperature is about at 15~30 DEG C.Its additive amount in feed is 50~100g/kg feed, specifically can be according to reality Border needs to be adjusted.
The basic principles, main features and advantages of the present invention have been shown and described above.The technology of the industry Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and the above embodiments and description only describe this The principle of invention, various changes and improvements may be made to the invention without departing from the spirit and scope of the present invention, these changes Change and improvement all fall within the protetion scope of the claimed invention.The claimed scope of the invention by appended claims and its Equivalent defines.

Claims (9)

1. a kind of nonreactive shrimp crab mulberry leaf liquid feed additive, which is characterized in that the component including following mass percent:
New fresh mulberry leaf slurries 60~70%;
Fresh feeding fly maggot slurries 25~35%;
Brown sugar water 4.5~5.5%;
Mixed bacteria 0.01~0.03%.
2. a kind of nonreactive shrimp crab mulberry leaf liquid feed additive according to claim 1, which is characterized in that
The nonreactive shrimp crab mulberry leaf liquid feed additive, the component including following mass percent:
New fresh mulberry leaf slurries 65%, fresh feeding fly maggot slurries 30%, brown sugar water 4.98%, mixed bacteria 0.02%.
3. a kind of nonreactive shrimp crab mulberry leaf liquid feed additive according to claim 1, which is characterized in that
The nonreactive shrimp crab mulberry leaf liquid feed additive, the component including following mass percent:
New fresh mulberry leaf slurries 62.5%, fresh feeding fly maggot slurries 32.5%, brown sugar water 4.97%, mixed bacteria 0.03%.
4. a kind of nonreactive shrimp crab mulberry leaf liquid feed additive according to claim 1 to 3, which is characterized in that
The mixed bacteria is saccharomycete, lactic acid bacteria, bacillus subtilis, denitrifying bacteria, at least three kinds mixed in photosynthetic bacteria It closes.
5. a kind of nonreactive shrimp crab mulberry leaf liquid feed additive according to claim 1 to 3, which is characterized in that
The fly maggot is fresh no-sundries fly maggot live larva.
6. a kind of preparation method of nonreactive shrimp crab mulberry leaf liquid feed additive, which is characterized in that specifically comprise the following steps:
S1, feedstock processing
New fresh mulberry leaf is cleaned, is drained, is beaten, is sieved that it is spare to obtain new fresh mulberry leaf slurries;
Fresh feeding fly maggot is also beaten, is sieved that it is spare to obtain feeding fly maggot slurries;
The brown sugar water for being tuned into 50~60% by brown sugar and water is spare;
S2, new fresh mulberry leaf slurries, feeding fly maggot slurries, brown sugar water and the mixed bacteria obtained after handling step S1 are proportionally It puts into constant temperature agitator tank reaction kettle, controls 25~35 DEG C of reaction temperature, while being that 25~40r/min stirring is anti-with mixing speed 8~12min is answered, mixed liquor is obtained;The mass percent of new fresh mulberry leaf slurries, feeding fly maggot slurries, brown sugar water and mixed bacteria It is as follows:
New fresh mulberry leaf slurries 60~70%;
Fresh feeding fly maggot slurries 25~35%;
Brown sugar water 4.5~5.5%;
Mixed bacteria 0.01~0.03%;
S3, by obtained mixed liquor barrelling after after step S2 reaction, and after being sealed by fermentation to obtain the final product;If fermentation temperature is T, Then: when T < 15 DEG C, need to ferment 12~20 days;When 15 <≤T≤30 DEG C, need to ferment 8~12 days;Work as T > 30 DEG C, need to ferment 5~8d.
7. a kind of preparation method of nonreactive shrimp crab mulberry leaf liquid feed additive according to claim 6, which is characterized in that In step S1, slurries are obtained by 60~100 meshes respectively after new fresh mulberry leaf, fresh feeding fly maggot mashing.
8. nonreactive shrimp crab mulberry leaf liquid feed additive is in preparing feed for shrimp and crab described in Claims 1 to 5 any one Using, which is characterized in that the nonreactive shrimp crab mulberry leaf liquid feed additive is adjustable shrimp crab intestine microenvironment, improves shrimp crab To the digestibility of feed, enhance the disease resistance of shrimp crab;It is also adjustable and purifies water simultaneously, reduce water pollution and cause Shrimp crab lesion and death, thus the comprehensive quality and yield for improving shrimp crab.
9. application described in any one of claim 10, which is characterized in that the additive amount of the nonreactive shrimp crab mulberry leaf liquid feed additive For 50~100g/kg feed.
CN201811063859.1A 2018-09-12 2018-09-12 A kind of nonreactive shrimp crab mulberry leaf liquid feed additive and the preparation method and application thereof Pending CN109221606A (en)

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Application publication date: 20190118