CN108641976A - A kind of complex microorganism water purification agent and preparation method thereof - Google Patents
A kind of complex microorganism water purification agent and preparation method thereof Download PDFInfo
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- CN108641976A CN108641976A CN201810333692.XA CN201810333692A CN108641976A CN 108641976 A CN108641976 A CN 108641976A CN 201810333692 A CN201810333692 A CN 201810333692A CN 108641976 A CN108641976 A CN 108641976A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/341—Consortia of bacteria
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
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Abstract
The invention discloses a kind of complex microorganism water purification agents, are made of bacillus amyloliquefaciens, bacillus subtilis and bacillus licheniformis, and bacterium number quantity ratio is 0.8 1.2: 1.8 2.3: 1.8 2.3, and total bacteria count reaches 10,000,000,000/g.The present invention is made of bacillus amyloliquefaciens, bacillus subtilis, bacillus licheniformis cooperation, they compound the synergistic effect and synergistic function for playing microorganism together, intensive decomposition is carried out to organic matter, especially for water body turbid phenomenon, increase water transparency in a short time, the content for effectively reducing ammonia nitrogen, total phosphorus and organic matter achievees the purpose that purify water and repairs water body, quick in water remediation, the period is short and nontoxic, efficient, non-secondary pollution, is easy degradation.
Description
Technical field
The invention belongs to water-treatment technology fields, more particularly, to a kind of complex microorganism water purification agent and preparation method thereof.
Background technology
With the development of industry and science and technology, environmental protection is increasingly valued by people, industrial sewage purification row
It puts and has become certainty.For the poor area of water resource, purified industrial waste water enables to recycle seems increasingly important again.At present
The industrial water purification agent in China mainly has inorganic and organic two major classes, inorganic and organic polymer water purification agent to be more and more widely used
In the processing of various water quality.However it is while obtaining tremendous development, insecurity during use and is caused to environment
Secondary pollution also increasingly attract people's attention.Such as inorganic water purification agent, the Al made in water is added in water body in aluminium salt3+Increase,
Senile dementia can be caused；The addition of molysite makes water body color burn, exists if any sulfide in water body, will form mixture glue
Body is hardly formed precipitation.In addition, synthetic organic polymer water purification agent is difficult the presence for avoiding acrylamide, if being added in water body,
The acrylamide concentration in water body will be increased, acrylamide is extremely toxic substance again, is caused damages to health.Therefore, it develops
It is a kind of it is safe and non-toxic, flocculation activity is high, the purifying agent of non-secondary pollution, the health and environmental protection to the mankind have very heavy
The realistic meaning wanted.
With the development of biotechnology, microbial water-purifying agent comes into being.With traditional inorganic and organic synthesis macromolecule
Flocculant is compared, microbial water-purifying agent have the advantages that many unique properties and：It is degraded by microorganisms, nontoxic, safety
It is high；Non-secondary pollution.In addition, the microorganism overwhelming majority for generating water purification effect comes from the very close environment of human relation
In, resource is extremely abundant, and the method for acquisition is also fairly simple, of low cost.Therefore, microbial water-purifying agent is as a kind of efficiently peace
Full water purification agent urgently seeks a kind of today of the traditional water purification agent of purifying agent replacement in people, has obtained many researchers
Attention and approval.It is contemplated that microbial water-purifying agent will make thoroughly to eliminate to pollute to become a reality, it replaces organic and inorganic
It is trend of the times to synthesize high molecular water purifier, and microbial water-purifying agent is carried out research as high effect nontoxic water treatment agent of new generation to be opened
Hair, is a certainty feelings.But there is also following defects for current microbial water-purifying agent：Dosage is big, and effect is unstable, bacterium
Kind source is indefinite (such as commercially available), and use condition is limited, cannot meet all sewage disposals.
China Patent No. ZL201310755713.4, a kind of title " side of Biochemical method excess sludge production sludge protein
Method " discloses mixed using being made of bacillus subtilis, bacillus amyloliquefaciens, bacillus licheniformis and bacillus megaterium
Combined bacteria kind is for handling excess sludge production sludge protein.But said combination is also not up to most in the purifying water effect of treated sewage
It is good.
Invention content
The invention solves first technical problem be to provide a kind of complex microorganism water purification agent.The water purification agent can be natural
Degrade non-secondary pollution, can effectively purify water, and have the characteristics that it is nontoxic, safe efficient, to city domestic sewage handle
Etc. have good treatment effect.Water purification agent of the present invention is in addition to the organic matters such as protein degradation, starch, fat, the born of the same parents of generation
Outer product can reach biological flocculation, make bulky grain insoluble substance flocculation deposition in water.
The invention solves second technical problem be to provide a kind of preparation method of above-mentioned complex microorganism water purification agent.
In order to solve the above technical problems, the present invention adopts the following technical scheme that：
A kind of complex microorganism water purification agent, is made of bacillus amyloliquefaciens, bacillus subtilis and bacillus licheniformis,
Bacterium number quantity ratio is 0.8-1.2: 1.8-2.2: 1.8-2.2, and total bacteria count reaches 10,000,000,000/g.
As being further improved for technical solution, the bacillus amyloliquefaciens, bacillus subtilis and lichens gemma bar
The bacterium number quantity ratio of bacterium is 1: 2: 2.
As being further improved for technical solution, additive amount of the complex microbial community in sewage is 3-8ppm.
To solve above-mentioned second technical problem, a kind of above-mentioned preparation method of complex microorganism water purification agent of the present invention, packet
Include following steps：
S1, screened from the serious turbid water body of water quality, acquire bed mud and supernatant be inoculated in by water sample, centrifuging and taking supernatant
In nutrient broth medium, 28-31 DEG C, 200rpm enrichment cultures are for 24 hours；
S2, it takes the bacterium solution after enrichment culture to be serially diluted, bacterium solution 1mL is taken to be put into equipped with 9mL sterile waters and is placed with glass
In the 50mL triangular flasks of pearl, set on shaking table vibrate 20min so that microbial cell is disperseed, stand 30s 10-1Dilution；Use 1mL
Aseptic straw draws 10-1Dilution 1mL, move into equipped with 9mL sterile waters test tube in, suction blow 3 times, mixed bacteria liquid, 10-2
Dilution.And so on, serial dilution is made 10-3、10-4、10-5、10-6、10-7、10-8A series of dilution bacterium solution.Take 10-6、
10-7、10-8Dilution bacterium solution is coated on nutrient broth agar culture medium, and scribing line separation is to obtaining pure bacterial strain repeatedly；
S3, the dominant bacteria of separation is identified according to the physiological and biochemical property of bacterium, the colonial morphology from bacterium and life
Reason biochemical test determine dominant bacteria be bacillus amyloliquefaciens, bacillus subtilis or bacillus licheniformis after, by this 3 kinds of bacterium
Single strain fermentation is carried out, then bacillus amyloliquefaciens, bacillus subtilis, bacillus licheniformis mix in proportion, bacterium number
Quantity ratio is 0.8-1.2: 1.8-2.2: 1.8-2.2, and total bacteria count reaches 10,000,000,000/g.
Unless otherwise specified, each raw material in the present invention can be obtained by commercially available purchase, equipment used in the present invention
The conventional equipment in fields can be used or carried out with reference to the prior art of fields.
Compared with prior art, the present invention has the advantages that：
The present invention is made of bacillus amyloliquefaciens, bacillus subtilis, bacillus licheniformis cooperation, they are compounded
The synergistic effect and synergistic function for playing microorganism together carry out intensive decomposition to organic matter, existing especially for water body muddiness
As increasing water transparency in a short time, effectively reducing the content of ammonia nitrogen, total phosphorus and organic matter, reach to purify water and repair water
The purpose of body, it is quick in water remediation, the period is short and nontoxic, efficient, non-secondary pollution, be easy degradation.The present invention has needle
Property screens strain, using effect is notable, and accommodation is wider.
Specific implementation mode
In order to illustrate more clearly of the present invention, with reference to preferred embodiment, the present invention is described further.
A kind of complex microorganism water purification agent of the present invention, by bacillus amyloliquefaciens, bacillus subtilis and lichens gemma bar
Bacterium forms, and bacterium number quantity ratio is 0.8-1.2: 1.8-2.3: 1.8-2.3, and total bacteria count reaches 10,000,000,000/g.
The bacillus amyloliquefaciens can generate a variety of alpha-amylases and protease, with bacillus subtilis in form, training
It supports feature and physio-biochemical characteristics aspect is closely similar；Belong to facultative anaerobic bacteria, bacterium colony is on LB culture mediums and beef extract-peptone
It is in faint yellow opaque colony on culture medium, rough surface has protuberance, and edge is irregular, not chromogenic on a variety of culture mediums
Element；There is mycoderm to be formed when Liquid Culture is static；Gram's staining is positive, rod-shaped, can form endogenous spore, oval, and two
Blunt circle, sporangium is held not to expand, middle life has motility to time end life；Hydrolyze starch and gelatin, acetyl methyl carbinol (V-P) examination
Feminine gender is tested, nitrate reduction test is negative, phenylalanine deaminase experiment, indole test, methyl red (MR) experiment, hydrogen sulfide examination
It is feminine gender to test.The bacterium is extensive in distributed in nature, easily separated culture, nontoxic to people and animals, free from environmental pollution.It is metabolized production
Object has broad spectrum antibiotic activity and stronger anti-adversity ability, growth is fast, and stability is preferable compared with horn of plenty.Lee is superfine from Dian Chi richness
Isolated 1 bacillus amyloliquefaciens DC1 in nutritive water, and its effect of algae restraint and mechanism to anabena have been inquired into,
It was found that bacillus amyloliquefaciens DC1 cell concentrations are bigger, more apparent to the removal effect of anabena, main algal control mechanism is logical
It crosses and secretes the indirect mode of extracellular substance and play algae-lysing, which has very strong thermal stability, is non-protein object
Matter.Tan Wenjie etc. has studied degradation effect of the bacillus amyloliquefaciens to butachlor in water, finds butachlor initial mass concentration
Higher, degradation rate and degradation efficiency are also higher, and the degradation rate of butachlor is obviously higher than under acid condition under alkaline condition, rotten
Grow acid has prodigious facilitation to the microbial degradation of butachlor.Tall and big sound etc. is separated to 1 plant of solution starch gemma from chicken manure
Bacillus has deodorization.
The bacillus subtilis morphological feature has typical bacillus feature for biological characteristics, thin
Born of the same parents are in direct rod shape, and size (0.8-1.2) μ m (1.5-4.0) μm, individually, Gram-positive, uniform coloring can produce pod
Film moves (peritrichous)；It is raw or close middle raw in gemma, it is wide less than or equal to cell, in oval to cylindric；Bacterium colony is coarse,
It is opaque, expansion, dirty white or micro-strip yellow；Can liquefy gelatin, peptonized milk, restore nitrate, hydrolyze starch, be typical good
Oxygen bacterium.The heat-resisting resistance of bacillus subtilis is strong, nontoxic to people and animals in the surface generally existing of soil and plant, not dirty
Contaminate environment.Since the bacillus subtilis speed of growth is fast, nutritional need is simple, it is easy to survive, colonize and breed, no pathogenicity,
And a variety of enzymes and antibiotic can be secreted, but also with good fermentation basis, purposes is very extensive, can improve harmful indigo plant
Water turbidity problem caused by algae is overflowed, water quality become clear by muddy, have very strong Function For Purifying Water.Bacillus subtilis can produce
The raw gamma-polyglutamic acid with good flocculating property, can adsorb and be enriched with the pollutant and heavy metal in water body, to the net of water body
Change plays an important role.Ten thousand persons of outstanding talent etc. carry out chromium (VI) removal processing using gamma-polyglutamic acid flocculating agent to containing chromium (VI) waste water,
As a result it shows：PH value is that the additive amount of gamma-polyglutamic acid in 4,30mL, 30mg/L waste water is 1.5mL and 1%CaCl2Dosage is
When 2.4mL, chromium (VI) removal effect is best, and chromium (VI) removal rate is up to 55%.In addition, the researchs such as ten thousand persons of outstanding talent also found and utilize paddy
Propylhomoserin waste water culture bacillus subtilis can effectively adsorb the flocculant of (Cr (VI)) with output；Flocculant mainly by γ-PGA and
Polysaccharide forms, and flocculant maximum Cr (VI) adsorption capacity after fixing is 4.2mg/g, and best Cr (VI) absorption pH value is 4.
The bacillus licheniformis is the gram-positive aerobic bacteria of central spore, it can decompose poisonous and harmful in water body
Substance purifies water.Hu Hongbo etc. shows ground using the result of study of the heavy metals such as microorganism adsorption recycling gold, silver, platinum, lead
The dead thalline of clothing bacillus R08 adsorb Pd2+Highest adsorbance can reach every gram of thalline absorption 224.8mg Pd2+。
In some embodiments of the invention, the bacillus amyloliquefaciens, bacillus subtilis and bacillus licheniformis
Bacterium number quantity ratio is 1: 2: 2.
In some embodiments of the invention, additive amount of the complex microbial community in sewage is 3-8ppm.
The present invention according to special ratios in practice, it has been found that use the bacillus subtilis of the invention combined, solution starch bud
Spore bacillus and bacillus licheniformis, than using bacillus subtilis, bacillus amyloliquefaciens, bacillus licheniformis and huge gemma
The combination of bacillus has better effect instead.
A kind of above-mentioned preparation method of complex microorganism water purification agent of the present invention, includes the following steps：
S1, screened from the serious turbid water body of water quality, acquire bed mud and supernatant be inoculated in by water sample, centrifuging and taking supernatant
In nutrient broth medium, 28-31 DEG C, 200rpm enrichment cultures are for 24 hours；
S2, it takes the bacterium solution after enrichment culture to be serially diluted, bacterium solution 1mL is taken to be put into equipped with 9mL sterile waters and is placed with glass
In the 50mL triangular flasks of pearl, set on shaking table vibrate 20min so that microbial cell is disperseed, stand 30s 10-1Dilution；Use 1mL
Aseptic straw draws 10-1Dilution 1mL, move into equipped with 9mL sterile waters test tube in, suction blow 3 times, mixed bacteria liquid, 10-2
Dilution.And so on, serial dilution is made 10-3、10-4、10-5、10-6、10-7、10-8A series of dilution bacterium solution.Take 10-6、
10-7、10-8Dilution bacterium solution is coated on nutrient broth agar culture medium, and scribing line separation is to obtaining pure bacterial strain repeatedly；
S3, the dominant bacteria of separation is identified according to the physiological and biochemical property of bacterium, the colonial morphology from bacterium and life
Reason biochemical test determine dominant bacteria be bacillus amyloliquefaciens, bacillus subtilis or bacillus licheniformis after, by this 3 kinds of bacterium
Single strain fermentation is carried out, then bacillus amyloliquefaciens, bacillus subtilis, bacillus licheniformis mix in proportion, bacterium number
Quantity ratio is 0.8-1.2: 1.8-2.2: 1.8-2.2, and total bacteria count reaches 10,000,000,000/g.
Embodiment 1
A kind of complex microorganism water purification agent is grouped as by the group of following bacterium number quantitative proportion：Bacillus amyloliquefaciens, withered grass
Bacillus, bacillus licheniformis, bacterium number quantity ratio are 1: 2: 2, additive amount 5ppm.
The preparation method of above-mentioned complex microorganism water purification agent, includes the following steps：
S1, screened from the serious turbid water body of water quality, acquire bed mud and supernatant be inoculated in by water sample, centrifuging and taking supernatant
In nutrient broth medium, 28-31 DEG C, 200rpm enrichment cultures are for 24 hours；
S2, it takes the bacterium solution after enrichment culture to be serially diluted, bacterium solution 1mL is taken to be put into equipped with 9mL sterile waters and is placed with glass
In the 50mL triangular flasks of pearl, set on shaking table vibrate 20min so that microbial cell is disperseed, stand 30s 10-1Dilution；Use 1mL
Aseptic straw draws 10-1Dilution 1mL, move into equipped with 9mL sterile waters test tube in, suction blow 3 times, mixed bacteria liquid, 10-2
Dilution.And so on, serial dilution is made 10-3、10-4、10-5、10-6、10-7、10-8A series of dilution bacterium solution.Take 10-6、
10-7、10-8Dilution bacterium solution is coated on nutrient broth agar culture medium, and scribing line separation is to obtaining pure bacterial strain repeatedly；
S3, the dominant bacteria of separation is identified according to the physiological and biochemical property of bacterium, the colonial morphology from bacterium and life
Reason biochemical test determine dominant bacteria be bacillus amyloliquefaciens, bacillus subtilis or bacillus licheniformis after, by this 3 kinds of bacterium
Single strain fermentation is carried out, then bacillus amyloliquefaciens, bacillus subtilis, bacillus licheniformis mix in proportion, bacterium number
Quantity ratio is 1: 2: 2, and total bacteria count reaches 10,000,000,000/g.
Comparative example 1
Embodiment 1 is repeated, the difference is that：A kind of complex microorganism water purification agent, including following bacterium number quantitative proportion
Component：Bacillus amyloliquefaciens, bacillus subtilis, bacillus licheniformis and bacillus megaterium, bacterium number quantity ratio are 1: 2: 2
: 2, additive amount 5ppm.
Comparative example 2
Embodiment 1 is repeated, the difference is that：A kind of complex microorganism water purification agent, including following bacterium number quantitative proportion
Component：Bacillus amyloliquefaciens, bacillus licheniformis, bacterium number quantity ratio are 1: 2, additive amount 5ppm.
Comparative example 3
Embodiment 1 is repeated, the difference is that：A kind of complex microorganism water purification agent, including following bacterium number quantitative proportion
Component：Bacillus subtilis, bacillus licheniformis, bacterium number quantity ratio are 1: 1, additive amount 5ppm.
Comparative example 4
Embodiment 1 is repeated, the difference is that：A kind of complex microorganism water purification agent, including following bacterium number quantitative proportion
Component：Bacillus subtilis, bacillus amyloliquefaciens, bacillus licheniformis, bacillus megaterium, each strain quality portion rate are
5: 3: 5: 3, additive amount is mass ratio 3%.
Comparative example 5
Embodiment 1 is repeated, the difference is that：A kind of complex microorganism water purification agent, including following bacterium number quantitative proportion
Component：Bacillus subtilis, bacillus amyloliquefaciens, bacillus licheniformis, bacillus megaterium, each strain quality portion rate are
8: 5: 5: 5, additive amount is mass ratio 5%.
Laboratory report：
1, Tangshan somewhere cyanobacteria grows water body
1) three groups are set：Blank group, control group, of the present invention group.
Blank group does not launch any inorganic agent；
Control group 1, which is launched, presses comparative example 1；Control group 2, which is launched, presses comparative example 4；Control group 3, which is launched, presses comparative example 5；
Of the present invention group of dispensing embodiment 1.
2) result：
Of the present invention group of transparency, dissolved oxygen are apparently higher than blank group and control group, NH3- N, total phosphorus are significantly lower than blank
Group and control group.
Each group transparency, dissolved oxygen, NH3The indexs such as-N, total phosphorus
| Blank group | Control group 1 | Control group 2 | Control group 3 | Of the present invention group | |
| Transparency (m) | 0.3 | 0.5 | 0.4 | 0.5 | 0.7 |
| Dissolved oxygen (mg/L) | 1.9 | 2.7 | 2.8 | 2.6 | 5.0 |
| NH3-N(mg/L) | 2.40 | 0.93 | 0.75 | 0.85 | 0.30 |
| Total phosphorus (in terms of P, mg/L) | 0.66 | 0.25 | 0.28 | 0.23 | 0.04 |
Note：Transparency, which measures, uses Sai Shi disk methods；DO determination uses iodimetric titration；NH3- N, which is measured, uses nessler reagent
Colorimetric method；Total phosphorus determination uses ammonium molybdate spectrophotometric method.
It can be seen that the combination of the present invention is on the basis of existing technology, reduce bacillus megaterium, transparency,
Dissolved oxygen, NH3The indexs such as-N, total phosphorus have better effect instead.
2, the serious turbid water body of Beijing somewhere water quality
1) three groups are set：Blank group, control group, of the present invention group.
Blank group does not launch any inorganic agent；
Control group 1, which is launched, presses comparative example 2；Control group 2, which is launched, presses comparative example 4；Control group 3, which is launched, presses comparative example 5；
Of the present invention group of dispensing embodiment 1.
2) result：
Of the present invention group of transparency, dissolved oxygen are apparently higher than blank group and control group, NH3- N, total phosphorus are significantly lower than blank
Group and control group.
Each group transparency, dissolved oxygen, NH3The indexs such as-N, total phosphorus
Note：Transparency, which measures, uses Sai Shi disk methods；DO determination uses iodimetric titration；NH3- N, which is measured, uses nessler reagent
Colorimetric method；Total phosphorus determination uses ammonium molybdate spectrophotometric method.
3, Tianjin somewhere water quality black and odorous water
1) three groups are set：Blank group, control group, of the present invention group.
Blank group does not launch any inorganic agent；
Control group 1, which is launched, presses comparative example 3；Control group 2, which is launched, presses comparative example 4；Control group 3, which is launched, presses comparative example 5；
Of the present invention group of dispensing embodiment 1.
2) result：
Of the present invention group of transparency, dissolved oxygen are apparently higher than blank group and control group, NH3- N, total phosphorus are significantly lower than blank
Group and control group.
Each group transparency, dissolved oxygen, NH3The indexs such as-N, total phosphorus
| Blank group | Control group 1 | Control group 2 | Control group 3 | Of the present invention group | |
| Transparency (m) | 0.2 | 0.3 | 0.4 | 0.4 | 0.6 |
| Dissolved oxygen (mg/L) | 0.5 | 1.9 | 2.5 | 2.3 | 4.0 |
| NH3-N(mg/L) | 8.05 | 2.80 | 1.95 | 2.25 | 0.35 |
| Total phosphorus (in terms of P, mg/L) | 0.82 | 0.33 | 0.20 | 0.25 | 0.03 |
Note：Transparency, which measures, uses Sai Shi disk methods；DO determination uses iodimetric titration；NH3- N, which is measured, uses nessler reagent
Colorimetric method；Total phosphorus determination uses ammonium molybdate spectrophotometric method.
The present invention is made of bacillus amyloliquefaciens, bacillus subtilis, bacillus licheniformis cooperation, they are compounded
The synergistic effect and synergistic function of microorganism are played together, and effect had both been better than the combination for being further added by strain, and had also been better than only two
The combination of kind strain.
Obviously, the above embodiment of the present invention be only to clearly illustrate example of the present invention, and not be pair
The restriction of embodiments of the present invention.For those of ordinary skill in the art, may be used also on the basis of the above description
To make other variations or changes in different ways.Here all embodiments can not be exhaustive.It is every to belong to this hair
Row of the obvious changes or variations that bright technical solution is extended out still in protection scope of the present invention.
Claims (5)
1. a kind of complex microorganism water purification agent, it is characterised in that：By bacillus amyloliquefaciens, bacillus subtilis and lichens gemma
Bacillus forms, and bacterium number quantity ratio is 0.8-1.2: 1.8-2.3: 1.8-2.3, and total bacteria count reaches 10,000,000,000/g.
2. complex microorganism water purification agent according to claim 1, it is characterised in that：The bacillus amyloliquefaciens, withered grass bud
The bacterium number quantity ratio of spore bacillus and bacillus licheniformis is 1: 2: 2.
3. complex microorganism water purification agent according to claim 1, it is characterised in that：The complex microbial community is in sewage
Additive amount be 3-8ppm.
4. according to the preparation method of any complex microorganism water purification agent in claim 1-3, which is characterized in that including as follows
Step：
S1, screened from the serious turbid water body of water quality, acquire bed mud and supernatant be inoculated in nutrition by water sample, centrifuging and taking supernatant
In broth bouillon, 28-31 DEG C, 200rpm enrichment cultures are for 24 hours；
S2, it takes the bacterium solution after enrichment culture to be serially diluted, bacterium solution 1mL is taken to be put into equipped with 9mL sterile waters and is placed with bead
In 50mL triangular flasks, set on shaking table vibrate 20min so that microbial cell is disperseed, stand 30s 10-1Dilution；It is sterile with 1mL
Suction pipe draws 10-1Dilution 1mL, move into equipped with 9mL sterile waters test tube in, suction blow 3 times, mixed bacteria liquid, 10-2Dilution
Liquid.And so on, serial dilution is made 10-3、10-4、10-5、10-6、10-7、10-8A series of dilution bacterium solution.Take 10-6、10-7、10-8Dilution bacterium solution is coated on nutrient broth agar culture medium, and scribing line separation is to obtaining pure bacterial strain repeatedly；
S3, the dominant bacteria of separation is identified according to the physiological and biochemical property of bacterium, is given birth to from the colonial morphology and physiology of bacterium
Change experiment determine dominant bacteria be bacillus amyloliquefaciens, bacillus subtilis or bacillus licheniformis after, by this 3 kinds of bacterium into
Row single strain ferments, and then bacillus amyloliquefaciens, bacillus subtilis, bacillus licheniformis mix in proportion, bacterium number quantity
Than being 0.8-1.2: 1.8-2.2: 1.8-2.2, total bacteria count reaches 10,000,000,000/g.
5. the preparation method of complex microorganism water purification agent according to claim 4, it is characterised in that：In step S3, the solution
Bacillus amyloliquefaciens, bacillus subtilis and the bacterium number of bacillus licheniformis quantity ratio are 1: 2: 2.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109231505A (en) * | 2018-11-20 | 2019-01-18 | 安徽省农业科学院水产研究所 | A kind of preparation method of stick flower fish and water quality improving agent |
| CN110804565A (en) * | 2019-11-21 | 2020-02-18 | 河北建设集团安装工程有限公司 | Microbial agent for improving total phosphorus index of polluted water body and preparation method and application thereof |
| CN111100830A (en) * | 2020-03-09 | 2020-05-05 | 闽江学院 | Composite microecological preparation for aquaculture water purification and application thereof |
| CN111924979A (en) * | 2020-07-27 | 2020-11-13 | 天津开发区坤禾生物技术有限公司 | Method for preventing and treating anorexia of fishes |
| CN115094005A (en) * | 2022-07-06 | 2022-09-23 | 四川大学 | Bacillus subtilis, biological material and application in hexavalent chromium pollution treatment |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104593303A (en) * | 2015-01-21 | 2015-05-06 | 威海金中元生物肥有限公司 | Liquid composite microbial agent and production method thereof |
| CN105858909A (en) * | 2016-04-05 | 2016-08-17 | 浙江卫绿环境科技有限公司 | Microbial herbal composite preparation for aquiculture and preparation method thereof |
| CN106630187A (en) * | 2016-12-12 | 2017-05-10 | 东莞市红树林环保科技有限公司 | Lake and landscape water ecological restoration method |
-
2018
- 2018-04-13 CN CN201810333692.XA patent/CN108641976A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104593303A (en) * | 2015-01-21 | 2015-05-06 | 威海金中元生物肥有限公司 | Liquid composite microbial agent and production method thereof |
| CN105858909A (en) * | 2016-04-05 | 2016-08-17 | 浙江卫绿环境科技有限公司 | Microbial herbal composite preparation for aquiculture and preparation method thereof |
| CN106630187A (en) * | 2016-12-12 | 2017-05-10 | 东莞市红树林环保科技有限公司 | Lake and landscape water ecological restoration method |
Non-Patent Citations (1)
| Title |
|---|
| W LIU,ET AL: "Isolation,identification and characterization of Bacillus amyloliquefaciens BZ-6,a bacterial isolate ofr enhancing oil recovery from ioly sludge", 《CHEMOSPHERE》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109231505A (en) * | 2018-11-20 | 2019-01-18 | 安徽省农业科学院水产研究所 | A kind of preparation method of stick flower fish and water quality improving agent |
| CN110804565A (en) * | 2019-11-21 | 2020-02-18 | 河北建设集团安装工程有限公司 | Microbial agent for improving total phosphorus index of polluted water body and preparation method and application thereof |
| CN111100830A (en) * | 2020-03-09 | 2020-05-05 | 闽江学院 | Composite microecological preparation for aquaculture water purification and application thereof |
| CN111924979A (en) * | 2020-07-27 | 2020-11-13 | 天津开发区坤禾生物技术有限公司 | Method for preventing and treating anorexia of fishes |
| CN115094005A (en) * | 2022-07-06 | 2022-09-23 | 四川大学 | Bacillus subtilis, biological material and application in hexavalent chromium pollution treatment |
| CN115094005B (en) * | 2022-07-06 | 2023-03-31 | 四川大学 | Bacillus subtilis, biological material and application in hexavalent chromium pollution treatment |
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