CN106361955A

CN106361955A - Preparation method of Chinese herbal medicinal active fermentation broth for preventing bacterial diseases in fishes and shrimps

Info

Publication number: CN106361955A
Application number: CN201610745917.3A
Authority: CN
Inventors: 蒋常德; 胡艳晖
Original assignee: Foshan Yanhui Biotechnology Co Ltd
Current assignee: Foshan Yanhui Biotechnology Co Ltd
Priority date: 2016-08-29
Filing date: 2016-08-29
Publication date: 2017-02-01

Abstract

The invention provides a Chinese herbal medicinal active fermentation broth for preventing bacterial diseases in fishes and shrimps and a preparation method of the Chinese herbal medicinal active fermentation broth. The invention provides a method for preventing the bacterial diseases in the fishes and shrimps by taking probiotic-fermented Chinese herbal medicines as feed additives. The Chinese herbal medicines include radix astragali, radix codonopsis, herba opuntiae, rhizoma coptidis, radix scutellariae, radix isatidis, fructus gardeniae, herba andrographitis, leaves of eucalyptus robusta smith, rhizoma anemarrhenae, cortex moutan, radix platycodonis, raw hawthorn, radix aucklandiae and the like. The feed additives for preventing the bacterial diseases in the fishes and shrimps are prepared by selecting the Chinese herbal medicines, crushing the selected Chinese herbal medicines, soaking the crushed Chinese herbal medicines, conducting decoction and conducting fermentation; and in a fermenting process, adopted effective probiotics mainly include bacillus tequilensis, streptomyces violaceus, saccharomyces boulardii, lactobacillus bulgaricus, rhodopseudomonas spheroides and the like. The Chinese herbal medicinal active fermentation broth provided by the invention has functions of preventing the bacterial diseases in the fishes and shrimps as well as promoting growth and boosting immunity, and the Chinese herbal medicinal active fermentation broth has the advantages of being easily used, low in dosage of the Chinese herbal medicines, high in content of living bacteria, free from residues, free from drug resistance and the like.

Description

A kind of preparation method of the Chinese herbal medicinal active fermentation broth of prevention fish and shrimp bacterial diseases

Technical field

The present invention relates to a kind of Chinese herbal medicinal active fermentation broth of prevention fish and shrimp bacterial diseases and preparation method thereof, particularly relate to And a kind of using what beneficial organism microflora fermentation vegetable Chinese herbal medicine was made, there is prevention high-efficiency activated of fish and shrimp bacterial diseases effect Zymotic fluid and preparation method thereof.

Background technology

In recent decades, the feedstuff medicated premix based on antibiotic, hormone, preservative is to culture fisheries such as fish and shrimp Produce and bring very big benefit.However, with their extensive application, also occurring in that some drawbacks, such as Resistant strain Produce, residual in poultry, fish and shrimp aquatic products for the antibiotic and superinfection etc., these problems are subject to the generally pass of international community Note and worry, more and more country has been prevented or restricted from Multiple Classes of Antibiotics, hormone, preservative one after another as feed additive. The application of the medicated feed additives such as antibiotic, hormone, preservative is subject to serious challenging.Therefore, develop and both promote one kind Can effectively substitute antibiotics, hormone, preservative again can health care, epidemic prevention, somatotrophic novel green feed additive be effectively Become the task of top priority.The disease problem of fish and shrimp bacteriosises (as vibrio) is the bottle hindering raising fish and shrimp industry sustainable development all the time Neck restricts, the raising of the fast development in particular with raising fish and shrimp and intensive culture degree, and is fed using man-made feeds Conventional disease-resistant chemical combination medicine such as antibiotic, hormone, preservative have the shortcoming being relatively also easy to produce drug resistance and large side effects again.And In medical domain, Chinese medicine can be confirmed with virus with antibacterium, energy antibacterium is many with the Chinese herbal medicine of virus by clinical and experiment Belong to diaphoretic medicine and antipyretic.Exterior syndrome is equivalent to the clinical upper respiratory tract infection of modern medicine and the symptom at infectious disease initial stage；In Heat syndrome then mainly includes the various acute infectious disease of modern medicine, infectious febrile disease.Chinese herbal medicine can by diaphoresis, antipyretic, Sedation and analgesia, relieving cough and expelling phlegm are relievingd asthma, antiinflammatory, antiallergic, antibacterial, antiviral and promote body's immunity (diaphoretic medicine) and disease-resistant Pathogenic microorganism, antibacterium toxin, antipyretic and anti-inflammatory, promote body's immunity and anticoagulant, anti-dic, improve hemorheological property, guarantor The pharmacological actions such as liver function of gallbladder promoting (antipyretic) reach the purpose preventing and treating influenza disease.The patent of existing preventing and treating fish and shrimp bacterial diseases is substantially all It is by Chinese herbal medicine or to be directly added in feedstuff or carry out taking extraction, but it is low all to there is active ingredient utilization rate, addition height etc. Shortcoming.

Directly utilize beneficial flora fermentation of Chinese herbal medicine, the cell wall of Chinese herbal medicine can be destroyed, greatly improve the effective of Chinese herbal medicine The utilization rate of composition, and produce multiple somatomedins and probiotic bacteria during the fermentation, the growth promoter of fish and shrimp can be had Effect improves meat, improves premunition, effect of enhancing immunity, and has easy to use, medium-height grass survival dose is few, and viable bacteria content is high And there is noresidue, have no drug resistance；Effect in conjunction with Chinese herbal medicine and probiotic bacteria.

Content of the invention

It is an object of the invention to provide having Chinese herbal medicinal active fermentation broth and its preparation side of prevention fish and shrimp bacterial diseases effect Method.

Another object of the present invention improves the utilization rate of the active ingredient of Chinese herbal medicine, reduces the consumption of Chinese herbal medicine.

Another object of the present invention provides Chinese herbal medicinal active fermentation broth of the present invention in the application of prevention fish and shrimp bacterial diseases.

It is on the basis of a large amount of investigation that the present invention is used for preventing the Chinese herbal medicinal active fermentation broth of fish and shrimp bacterial diseases, according to product The design function of product requires more than 220 kinds of Chinese herbal medicine are screened, according to the monarch of traditional Chinese medicine, seven emotions compatibility, ten Eight is anti-, nineteen medicaments of mutual restraint scheduling theory, screens one by one and the effective component of Chinese herbal medicine filtering out, bacteriostasis are carried out extracting, verify, Certain formula eventually.Filter out from 420 multiple beneficial microorganisms again 5 kinds can symbiotic co-existence, and medium-height grass can be greatly improved The utilization rate of the active ingredient of medicine, produce during the fermentation multiple somatomedins probiotic bacteria.

To achieve these goals, the present invention employs the following technical solutions:

A kind of Chinese herbal medicinal active fermentation broth of prevention fish and shrimp bacterial diseases, is made up of the raw material of following weight portion: Radix Astragali 3-10 part, party Ginseng 5-10 part, Radix et Caulis Opuntiae Dillenii 6-12 part, Rhizoma Coptidis 4-8 part, Radix Scutellariae 4-8 part, Radix Isatidis 5-10 part, Fructus Gardeniae 5-10 part, Herba Andrographis 5-10 Part, Folium Eucalypti Robustae 5-10 part, Rhizoma Anemarrhenae 5-10 part, Cortex Moutan 5-10 part, Radix Platycodoniss 5-10 part, Fructus Crataegi 12-30 part, Radix Aucklandiae 12-20 part, sugar Sweet 40-60 part, 850 parts of water, 10,000,000,000 cfu/ml Te Jila bacillus cereuss 5-15 parts, 100,000,000 cfu/ml Streptomyces violaceus 5-15 parts, 1000000000 cfu/ml cloth Laplace yeast 5-15 parts, 10,000,000,000 cfu/ml Lactobacillus bulgaricus 5-15 parts, 1,000,000,000 cfu/ml are spherical red Pseudomonass 5-15 part；By size-reduced for said herbal medicine, immersion, heating and thermal insulation, fermentation and be obtained prevention fish and shrimp bacterial diseases in Medical herbs active-fermented broth.

Wherein, the preparation method of the Chinese herbal medicinal active fermentation broth of prevention fish and shrimp bacterial diseases, comprises the steps:

(1), various Chinese herbal medicine commercially are individually ground into 100 mesh fine powders；

(2), sequentially add in fermentation tank: Radix Astragali 3-10 part, Radix Codonopsis 5-10 part, Radix et Caulis Opuntiae Dillenii 6-12 part, Rhizoma Coptidis 4-8 part, Radix Scutellariae 4- 8 parts, Radix Isatidis 5-10 part, Fructus Gardeniae 5-10 part, Herba Andrographis 5-10 part, Folium Eucalypti Robustae 5-10 part, Rhizoma Anemarrhenae 5-10 part, Cortex Moutan 5-10 part, Radix Platycodoniss 5-10 part, Fructus Crataegi 12-30 part, Radix Aucklandiae 12-20 part, molasses 40-60 part, 850 parts of water.After soaking 30 minutes, it is heated to 95 DEG C, it is incubated two hours, is cooled to 30 DEG C；

(3), the coolant (2) produced in step adds 10,000,000,000 cfu/ml Te Jila bacillus cereuss 5-15 parts, 100,000,000 cfu/ml purples Streptomycete 5-15 part, 1,000,000,000 cfu/ml cloth Laplace yeast 5-15 parts；30 DEG C -33 DEG C, open stirring 120-160r/min, per minute Logical filtrated air amount and fermentating liquid volume are than for 1:1-1.5:1；After fermentation 36 hours, then add 10,000,000,000 cfu/ in fermentation tank Ml Lactobacillus bulgaricus 5-15 part, 1,000,000,000 cfu/ml Rhodopseudomonas spheroidess 5-15 parts, 30-35 DEG C of static gas wave refrigerator 120-150 Hour, detect that its ph is less than 4.0, viable bacteria content reaches 1,000,000,000 cfu/ml, you can terminate fermentation；

(4), release bacterium solution, packed products.

Wherein, the using method of prevention fish and shrimp bacterial diseases is to be added in daily feedstuff to stir, and adding proportion controls In 0.1%-0.2%；Or direct full pond water-sprinkling, addition is 1-2l/ mu.

Wherein, step (3) in Te Jila bacillus cereuss, Streptomyces violaceus, cloth Laplace yeast, bulgarian milk bar Bacterium, the production of Rhodopseudomonas spheroidess can adopt prior art, present invention offer following methods:

1., the preparation of Te Jila bacillus cereuss liquid strain

Take out culture presevation pipe, draw flat board with lb solid medium and recovered, cultivate 48 hours for 30 DEG C.Picking list under flat board In individual 50 milliliters of lb culture medium of colony inoculation, 30 DEG C of concussion and cultivates 24 hours in incubator.Seed adopts 5% inoculum concentration, connects Plant to the big triangular flask of 5l equipped with the Te Jila bacillus cereuss liquid bacterium culture medium of 2l, 30 DEG C of concussion and cultivate 20-28 hours, Detect its bacterial concentration, spore content is more than 10,000,000,000 cfu/ml, you can as the inoculation of Te Jila bacillus cereuss liquid strain.Its Described in lb solid medium: peptone 5g, Carnis Bovis seu Bubali cream 3g, sodium chloride 5g, agar 20 g, water 1000ml, ph7.2.Wherein institute State Te Jila bacillus cereuss liquid bacterium culture medium: glucose 5g/l, Semen Maydis powder 25 g/l, bean cake powder 30 g/l, magnesium sulfate 0.2 g/l, manganese sulfate 0.5 g/l, potassium dihydrogen phosphate 0.2 g/l, Calcium Carbonate 5 g/l, ph7.2.The condition of each medium sterilization For: 0.10-0.15mpa, 121 DEG C sterilize 30 minutes.

2., the preparation of Streptomyces violaceus strain

Take out culture presevation pipe, draw flat board with pda solid medium and recovered, cultivate 48 hours for 30 DEG C.Picking under flat board Single bacterium colony is rule to the Fructus Solani melongenae bottle equipped with pda solid medium, cultivates 3-5 days for 30 DEG C in incubator, treats that strain produces After substantial amounts of spore, scraped with physiological saline solution, adjustment Streptomyces violaceus spore concentration is 100,000,000 cfu/ml, as purple chain Mold species.Wherein said pda solid medium: Rhizoma Solani tuber osi 200g, sucrose 20g, agar 20 g, water 1000ml.

3., the preparation of cloth Laplace yeast liquid body strain

Take out culture presevation pipe, draw flat board with pda solid medium and recovered, cultivate 48 hours for 30 DEG C.Picking under flat board Single bacterium colony is inoculated in 50 milliliters of pda culture medium, 30 DEG C of concussion and cultivates 24 hours in incubator.Seed adopts 5% inoculation Amount, is seeded in the big triangular flask of 5l equipped with the sub- culture medium of cloth Laplace barmses, and each liquid amount is 2l, 30 DEG C of concussion and cultivates 20-28 hour, detects its bacterial concentration, and bacterial content is more than 1,000,000,000 cfu/ml, you can connect as cloth Laplace yeast liquid body strain Kind.Wherein said pda solid medium: Rhizoma Solani tuber osi 200g, sucrose 20g, agar 20 g, water 1000ml.

The wherein said sub- culture medium of cloth Laplace barmses: glucose 20 g/l, yeast extract 5 g/l, peptone 5 g/ L, ammonium sulfate 5 g/l, magnesium sulfate 0.5 g/l.The condition of each medium sterilization is: 0.10-0.15mpa, and 121 DEG C sterilize 30 points Clock.

4., the preparation of Lactobacillus bulgaricus liquid spawn

Take out Lactobacillus bulgaricus culture presevation pipe, draw flat board with mrs solid medium and recovered, 37 DEG C of Anaerobic culturel 48 Hour.Under flat board, picking single bacterium colony is inoculated in 50 milliliters of mrs culture medium, 37 DEG C of Anaerobic culturel 24 hours in incubator. Seed adopts 5% inoculum concentration, is seeded to equipped with the big triangular flask of 10l of Lactobacillus bulgaricus seed culture medium, liquid amount is 8l, 37 DEG C of Anaerobic culturel 20-28 hours, detect its bacterial concentration, bacterial content is more than 10,000,000,000 cfu/ml, you can as Bao Jiali Sub- lactobacilluss liquid spawn inoculation.Wherein said mrs culture medium: peptone 10g/l, yeast extract 5 g/l, Carnis Bovis seu Bubali cream 10 G/l, glucose 20 g/l, Sodium Acetate Trihydrate 5 g/l, dibasic ammonium citrate 2 g/l, tween 80 1 ml/l, magnesium sulfate 0.6 g/l, sulfur Sour manganese 0.05 g/l, dipotassium hydrogen phosphate 2 g/l, agar 20 g/l, Calcium Carbonate 20 g/l.Wherein said Lactobacillus bulgaricus Seed culture medium: glucose 4%, peptone 1 %, Carnis Bovis seu Bubali cream 0.75%, sodium chloride 0.1%, manganese sulfate 0.001%, magnesium sulfate 0.02%, Sodium Acetate Trihydrate 0.05%, Calcium Carbonate 5%, ph6.0.The condition of each medium sterilization is: 0.10-0.15mpa, 121 DEG C of sterilizings 30 minutes.

5., the preparation of Rhodopseudomonas spheroidess liquid spawn

Take out Rhodopseudomonas spheroidess kind preservation pipe, draw flat board with Rhodopseudomonas spheroidess solid medium and recovered, in light Cultivate 72 hours according to 35 DEG C in incubator.Intensity of illumination: 1000lex-6000lex.Picking single bacterium colony inoculation 50 under flat board In milliliter Rhodopseudomonas spheroidess fluid medium, 35 DEG C of Anaerobic culturel 120 hours in illumination box.Seed adopts 10% Inoculum concentration, be seeded to the big triangular flask of 5l of the Rhodopseudomonas spheroidess fluid medium equipped with 4.5l, in illumination box 35 DEG C of Anaerobic culturel 72-96 hours, detect its bacterial concentration, bacterial content is more than 3,000,000,000 cfu/ml, you can as spherical red false single Born of the same parents' bacteria liquid strain seed is inoculated.Wherein said Rhodopseudomonas spheroidess solid medium: potassium dihydrogen phosphate 1g, calcium chloride 0.1g, sodium carbonate 3g, Sodium Acetate Trihydrate 1g, magnesium chloride 0.5g, ammonium chloride 1g, sodium chloride 1g, agar 20g, ph7.5, water 1000ml.Its Described in Rhodopseudomonas spheroidess fluid medium: potassium dihydrogen phosphate 1g, calcium chloride 0.1g, sodium bicarbonate 2g, Sodium Acetate Trihydrate 4g, chlorine Change magnesium 0.5g, ammonium chloride 1g, sodium chloride 1g, yeast extract 0.5g, ph7.5, water 1000ml.The condition of each medium sterilization is: 0.10-0.15mpa, 121 DEG C sterilize 30 minutes.

Cloth Laplace yeast (saccharomyces cerevisiae boulardii) is contained in the fermentation liquid of the present invention, It is a kind of non-toxic yeast of thermophilic.Hennequin etc. utilizes dna fragment to prove the ring of cloth Laplace yeast resistant against high temperatures high humidity Border.Cloth Laplace yeast is detached Yeasts from Fructus Litchi fruit, is confirmed to be a separation kind of beer yeast, it has Thermostability, can grow at a temperature of 37 DEG C.By many experiments it is understood that yeast not only under aerobic and anaerobic state all Can existence and multiply, also there is very strong inulinase-producing activity, can produce abundant b vitamin, Glutathione, ergosterol, The nutrient substance such as aminoacid and UGF.And pass through to study recently discovery cloth Laplace yeast and can also improve intestinal beneficial The activity of microorganism, suppression growth of pathogenic bacteria, to bacteriotoxic neutralization and Degradation to pathogen toxin.It is reported that it is stupid Solidity shuttle acid bacterium (c.difficile) colonizes in intestinal, produces two protein exotoxin, toxin a and toxin b, may result in liquid Body secretion, small intestinal inflammation, Traumatic Colon simultaneously can stimulate the release of inflammatory cytokine.And cloth Laplace yeast can secrete a hatching egg White enzyme.This protease degradable toxin a and colon hair cell marginal membrane (bbm) receptor being combined with toxin a, go back degradable poison Plain b, thus reducing the combination to colon for toxin a and b, suppresses the effect to colon epidermis cell for two toxin, and then to many diseases Originality enteritis has preventive and therapeutic action.Cloth Laplace yeast also can to bacteriotoxin produce neutralization, vibrio cholera ( V.cholerae) producible cholera toxin (cholera toxin, ct).Ct may result in serious diarrhoea.Cloth Laplace yeast The protein that size is 120 ku can be produced to excretion, this protein can be combined with cholera toxin, suppression cholera toxin is to adenosine The activation of cyclase of acid.The diarrhoea that treatment is caused by vibrio cholera thus can be reached, and then it is bacteriotoxic to reach neutralization Effect.

Streptomyces violaceus is contained, Streptomyces violaceus can produce abundant during fermentation of Chinese herbal medicine in the fermentation liquid of the present invention Cellulase, xylanase, the non-starch polysaccharide enzyme such as glucanase and amylase, protease, destroy the thin of Chinese herbal medicine The content of the active ingredient of Chinese herbal medicine can be improved several times, and anthracene nucleus pathogenic bacteria is killed in generation during the fermentation by cell wall Mycetin complex, also has the activity of stronger antiviral, organically combines effect of probiotic bacteria and Chinese herbal medicine, can greatly promote Enter the growth promoter of fish and shrimp, meat can be effectively improved, improve premunition, effect of enhancing immunity.

In fermentation liquid in the present invention contain Te Jila bacillus cereuss, Te Jila bacillus cereuss can produce enrich amylase and Protease, is that the ability that a kind of antibacterial with broad-spectrum antibacterial activity produces secondary metabolite is stronger, the antibiotic of its secretion, Antibacterial protein or polypeptides matter etc. play preferable Biological control effect.The process of Te Jila fermentation of bacillus Chinese herbal medicine In, with Chinese herbal medicine as culture medium, some new Chinese herbal medicine functional group active factorses and somatomedin can be synthesized, after testing, Its Antagonistic protein and lipopeptide antibiotic, polypeptides matter substantially improves more than 20% than general culture medium, strengthens it and controls Disease, the effect of diseases prevention；

Contain Rhodopseudomonas spheroidess in the present invention, be a kind of ancient thalline with very high nutritive value, individual minimum, but battalion Form point very abundant, it contains substantial amounts of protein and aminoacid, is a kind of high-quality protein source, and its thalline contains protein 63.8%th, fat 8.2%, soluble saccharide 20.8%, crude fibre 2.8%, ash 4.4%；Also contain multiple biological activities Point, carotene carotene content is 0.56mg/g, and the b vitamin 88mg/g(such as Ubidecarenone mg/g, vb1, vb2, vb6, vb12 is wherein Vb12 content is 200 times of yeast) and 16 kinds of essential amino acids, Folic Acid, biotin, ubiquinones etc., especially ubiquinone contains Amount is respectively 13 times, 94 times of the leaf of spinach, 82 times of Western corn of yeast.Separately have been reported that, Rhodopseudomonas spheroidess are rich in recklessly Radix Raphani element, at present, can extract 30 Carotenoids from its thalline, can as the good source of natural pigment, and have aobvious The antioxidation writing.Add appropriate Rhodopseudomonas spheroidess and effectively can convert harmful substance in breeding process, make The dissolved oxygen amount of culturing pool increases, and ph is suitable, and ammonia nitrogen, nitrate nitrogen reduce, and are beneficial to algae and the increase of microfauna quantity.In It is that fish, shellfish, shrimp etc. ingest normally, growth is rapid, and disease greatly reduces.Rhodopseudomonas spheroidess are in breeding water body Beneficial microbe, because they are to the pollution purification of the environment and protection of water quality, to fish, shrimp, the required nutrition of shellfish and important The supplement of biological active substanceies, thus make fish, shrimp, shellfish growth normal, resistance strengthens, minimizing of recuperating.Moreover, many Result of study shows, Rhodopseudomonas spheroidess except recuperating caused by effectively preventing and treating because of malnutrition, the such as nyctalopia of fish, also Certain prevention effect can be played to antibacterial and fungus-caused disease.

Substantial amounts of Lactobacillus bulgaricus are contained, Lactobacillus bulgaricus can produce multiple antibiotics in the present invention, these Antibiotics have antagonism to Salmonella, shigella, staphylococcuses, fusiform bacteria, pseudomonass etc., can using fermentation liquid Whole ph is less than 4, can play the effect of acidulant, increases palatability, improves intestine microenvironment, promotes poultry intestinal beneficial bacterium Propagation, stoped by the multiple antibiotics that fermentation produces or suppression harmful bacteria breeding, it is disorderly to correct intestinal flora, adjusts intestinal Colony balance；Suppression putrefaction bacteria, prevents the generation of harmful substance in intestinal.Promote animal productiong and improve production performance；Change Kind meat, improves product grade thus obtaining higher economic benefit.

The excellent effect of the present invention is: 1., technical scheme is by the research of Tiny ecosystem for many years and cultivation warp Test repetition test, meet the theory of Chinese medicine；It is to be sieved from more than 220 kinds of Chinese herbal medicine from invention Chinese herbal medicine formula Choosing, according to the monarch of traditional Chinese medicine, seven emotions compatibility, eighteen incompatible medicamentss, nineteen medicaments of mutual restraint scheduling theory, screening to filtering out one by one Effective component of Chinese herbal medicine, bacteriostasis carry out extracting, verify, finally determine formula, and being proved by probiotics fermention can be effectively pre- Anti- fish and shrimp bacterial diseases and its complication, and have somatotrophic effect well.

2., the Chinese herbal medicine in the Chinese herbal medicinal active fermentation broth of the present invention is in Te Jila bacillus cereuss, Streptomyces violaceus, Bradley Family name's yeast, Lactobacillus bulgaricus, under the Fermentation of Rhodopseudomonas spheroidess, destroy the cell wall of Chinese herbal medicine, can be by The content of the active ingredient of Chinese herbal medicine improves 2-4 times, strengthens the effect of prevention fish and shrimp bacterial diseases and its complication, and was fermenting Produce multiple antibiotic substance in journey and somatomedin such as kills the mycetin complex of anthracene nucleus pathogenic bacteria, organically combine Probiotic bacteria and effect of Chinese herbal medicine, can be greatly facilitated the growth promoter of fish and shrimp, can be effectively improved meat, improve premunition, increase Effect of strong immunity simultaneously has easy to use, and medium-height grass survival dose is few, and viable bacteria content is high and has noresidue, have no drug resistance Advantage.

3., the various probiotic bacterias in the present invention are to filter out 5 kinds from 420 multiple beneficial microorganisms can greatly improve The utilization rate of the active ingredient of medical herbs, and during the fermentation can symbiotic co-existence, promote mutually.It during the fermentation can be by The final ph of Chinese herbal medicinal active fermentation broth is less than 4, can play the effect of acidulant, increases palatability.Improve intestine microenvironment, Promote the propagation of animal and bird intestines probioticss, stop or suppression harmful bacteria breeding, correct intestinal flora disorderly.

, the fermentation liquid of the present invention there is obvious water transfer function, can harmful substance such as ammonia nitrogen in fast water purification body, nitrous Hydrochlorate, the mass degradation such as hydrogen sulfide, fast quick-recovery water colour.

Specific embodiment

Some that the following is the present invention implement examples, to be invented actually used are not limited to embodiment.

Embodiment 1, for preventing the preparation of the probiotic bacteria Chinese herbal medicinal active fermentation broth of fish and shrimp bacterial diseases

A kind of Chinese herbal medicinal active fermentation broth of prevention fish and shrimp bacterial diseases, is made up of the raw material of following weight portion: 8 parts of the Radix Astragali, Radix Codonopsis 8 Part, 10 parts of Radix et Caulis Opuntiae Dillenii, 6 parts of Rhizoma Coptidis, 6 parts of Radix Scutellariae, 8 parts of Radix Isatidis, 8 parts of Fructus Gardeniae, 8 parts of Herba Andrographis, 8 parts of Folium Eucalypti Robustae, 8 parts of the Rhizoma Anemarrhenae, 8 parts of Cortex Moutan, 6 parts of Radix Platycodoniss, 20 parts of Fructus Crataegi, 16 parts of the Radix Aucklandiae, 50 parts of molasses, 850 parts of water, 10,000,000,000 cfu/ml Te Jila spore bars 10 parts of bacterium, 100,000,000 10 parts of cfu/ml Streptomyces violaceus, 1,000,000,000 10 parts of cfu/ml cloth Laplace yeast, 10,000,000,000 cfu/ml Bulgaria 10 parts of lactobacilluss, 1,000,000,000 10 parts of cfu/ml Rhodopseudomonas spheroidess；By size-reduced for said herbal medicine, immersion, heating and thermal insulation, fermentation And the Chinese herbal medicinal active fermentation broth of prevention fish and shrimp bacterial diseases is obtained.

(2), sequentially add in fermentation tank: 8 parts of the Radix Astragali, 8 parts of Radix Codonopsis, 10 parts of Radix et Caulis Opuntiae Dillenii, 6 parts of Rhizoma Coptidis, 6 parts of Radix Scutellariae, Radix Isatidis 8 Part, 8 parts of Fructus Gardeniae, 8 parts of Herba Andrographis, 8 parts of Folium Eucalypti Robustae, 8 parts of the Rhizoma Anemarrhenae, 8 parts of Cortex Moutan, 6 parts of Radix Platycodoniss, 20 parts of Fructus Crataegi, 16 parts of the Radix Aucklandiae, sugar 50 parts of honey, 850 parts of water.After soaking 30 minutes, it is heated to 95 DEG C, be incubated two hours, be cooled to 30 DEG C；

(3), the coolant (2) produced in step adds 10 parts of 10,000,000,000 cfu/ml Te Jila bacillus cereuss, 100,000,000 cfu/ml purple chains 10 parts of mycete, 1,000,000,000 10 parts of cfu/ml cloth Laplace yeast；30 DEG C -33 DEG C, open stirring 120-160r/min, per minute logical aseptic Air capacity and fermentating liquid volume are than for 1:1-1.5:1；After fermentation 36 hours, then 10,000,000,000 cfu/ml guarantors are added to add in fermentation tank 10 parts of Leah lactobacilluss, 1,000,000,000 10 parts of cfu/ml Rhodopseudomonas spheroidess, 30-35 DEG C of static gas wave refrigerator 120-150 hour, detects it Ph is less than 4.0, and viable bacteria content reaches 1,000,000,000 cfu/ml, you can terminate fermentation；

(4), release bacterium solution, packed products.

Wherein, the using method of prevention fish and shrimp bacterial diseases is to be added in daily feedstuff to stir, and adding proportion controls In 0.1-0.2%；Or direct full pond water-sprinkling, addition is 1-2l/ mu；Also can spice use with full pond water-sprinkling simultaneously.

The result of the test of fish and shrimp bacterial diseases prevented and treated by fermentation liquid below with the present invention.

The controlling experiment result of the fermentation liquid prawn vibrio infection of embodiment 2 present invention

1st, test site: Zhanjiang City Leizhou City Ji Jia town；Breed variety: Penaeus vannamei；Area: ten mu；Culture style: soil The pool is raised together with；Put natural law in a suitable place to breed: 15 days；

2nd, problem description: prawn is in and turns the liver phase, extremely serious steathily, in shrimp body, hemolysis vibrion is more than 10⁶Cfu/g, ph8.0, dissolved oxygen 6.6mg/l, ammonia nitrogen 0.3mg/l, nitrite 0mg/l, hydrogen sulfide 0mg/l；

3rd, case study: should be prawn arc microbial prawn early stage to steal dead syndrome；

4th, solution: Chinese herbal medicinal active fermentation broth is added in daily feedstuff and stirs, adding proportion controls 0.2%； Simultaneously by active-fermented broth directly full pond water-sprinkling, addition is 2l/ mu.

5th, result: after active-fermented broth of the present invention uses, after 24 hours, extremely significantly reduce steathily, the detection of prawn body vibrio contains Amount has reduced an order of magnitude and has reached 2 × 10⁵cfu/g；After 48 hours, shrimp body colour improves, only a small amount of dead steathily；3rd day Afterwards, shrimp body colour is recovered, and no steals dead, prawn body vibrio detection level has reduced to 30cfu/g.

Embodiment 3

Prevention effect in Pelteobagrus fulvidraco quick-fried head disease for the fermentation liquid of the present invention

1st, test site: Hunan Province Longhui County raiser, Pelteobagrus fulvidraco cultured area reaches 300 mu, and body weight is in 1-3 two.

2nd, problem description: having fish quick-fried head disease is " Herba Duchesneae Indicae " disease, and dead fish amount increases substantially, using other parasite killing, resists Raw element medicine effect is inconspicuous, and water colour is more muddy, ammonia nitrogen 0.7mg/l, nitrite 0.2mg/l, hydrogen sulfide 0mg/l.

3rd, case study: how this disease is by the parasitogenic bacillary intercurrent disease of the parasites such as Trichodina.It is " Ai Dehuashi Bacterium " chronic infection causes.Its route of infection is via intranasal application infection olfactory receptor cell, enters back into brain, through infection of meninges skull.Should Disease is many to be occurred with other diseases, is most commonly that septicemia easily occurs after occurring splitting head disease.Because the infection of " Herba Duchesneae Indicae " disease It is in fish brain to brain, so once falling ill, external sterilizing, effects of antibiotics for oral administration are not very good.

4th, solution: Chinese herbal medicinal active fermentation broth is added in daily feedstuff and stirs, adding proportion controls 0.2%；Simultaneously by active-fermented broth directly full pond water-sprinkling, addition is 2l/ mu.

5th, result: after active-fermented broth of the present invention uses, after 24 hours, dead fish oneself lose and increase, water colour also has and substantially changes See, after 48 hours, dead fish significantly reduces；After 3rd day, fish body color recovers, no dead fish, and water colour is yellowish green, ammonia nitrogen 0.1mg/l, Nitrite 0mg/l, hydrogen sulfide 0mg/l, effect highly significant.

Claims

1. a kind of Chinese herbal medicinal active fermentation broth of prevention fish and shrimp bacterial diseases is it is characterised in that be made up of the raw material of following weight portion: Radix Astragali 3-10 part, Radix Codonopsis 5-10 part, Radix et Caulis Opuntiae Dillenii 6-12 part, Rhizoma Coptidis 4-8 part, Radix Scutellariae 4-8 part, Radix Isatidis 5-10 part, Fructus Gardeniae 5-10 Part, Herba Andrographis 5-10 part, Folium Eucalypti Robustae 5-10 part, Rhizoma Anemarrhenae 5-10 part, Cortex Moutan 5-10 part, Radix Platycodoniss 5-10 part, Fructus Crataegi 12-30 part, Radix Aucklandiae 12-20 part, molasses 40-60 part, 850 parts of water, 10,000,000,000 cfu/ml Te Jila bacillus cereuss 5-15 parts, 100,000,000 cfu/ml purples Streptomycete 5-15 part, 1,000,000,000 cfu/ml cloth Laplace yeast 5-15 parts, 10,000,000,000 cfu/ml Lactobacillus bulgaricus 5-15 parts, 10 Hundred million cfu/ml Rhodopseudomonas spheroidess 5-15 parts；By size-reduced for said herbal medicine, immersion, heating and thermal insulation, fermentation and be obtained prevention The Chinese herbal medicinal active fermentation broth of fish and shrimp bacterial diseases.

2. the preparation method of the Chinese herbal medicinal active fermentation broth of prevention fish and shrimp bacterial diseases according to claim 1, its feature exists In comprising the steps:

(2), sequentially add in fermentation tank: Radix Astragali 3-10 part, Radix Codonopsis 5-10 part, Radix et Caulis Opuntiae Dillenii 6-12 part, Rhizoma Coptidis 4-8 part, Radix Scutellariae 4- 8 parts, Radix Isatidis 5-10 part, Fructus Gardeniae 5-10 part, Herba Andrographis 5-10 part, Folium Eucalypti Robustae 5-10 part, Rhizoma Anemarrhenae 5-10 part, Cortex Moutan 5-10 part, Radix Platycodoniss 5-10 part, Fructus Crataegi 12-30 part, Radix Aucklandiae 12-20 part, molasses 40-60 part, 850 parts of water, after soaking 30 minutes, it is heated to 95 DEG C, it is incubated two hours, is cooled to 30 DEG C；

(4), release bacterium solution, packed products.

3. the Chinese herbal medicinal active fermentation broth of prevention fish and shrimp bacterial diseases according to claim 1 is it is characterised in that prevent fish and shrimp The using method of bacterial diseases is to be added in daily feedstuff to stir, and adding proportion controls in 0.1%-0.2%；Or direct full pond Water-sprinkling, addition is 1-2l/ mu.