CN109730308A - Tranquilizing the mind nourishing heart enhances memory effect Sialic acid complex polypeptide nutrition element and preparation method - Google Patents
Tranquilizing the mind nourishing heart enhances memory effect Sialic acid complex polypeptide nutrition element and preparation method Download PDFInfo
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Abstract
The invention discloses a kind of tranquilizing the mind nourishing heart enhancing memory effect Sialic acid complex polypeptide nutrition element, the raw materials including following parts by weight: 15 parts of Sialic acid, 15 parts of γ-aminobutyric acid, 10 parts of semen ziziphi spinosae, 15 parts of albumin polypeptide, 15 parts of phosphatidylserine, 15 parts of DHA powder, 15 parts of fermented ginseng powder, 60 parts of fermented whole eggs powder, 10 parts of Poria cocos, 10 parts of pueraria lobata, 10 parts of lotus seeds, 10 parts of cape jasmine, 10 parts of fingered citron, 10 parts of sanchi flower, 10 parts of semen coicis, 1 part of composite B vitamin, 1 part of xylo-oligosaccharide.The invention also discloses the preparation method and applications of tranquilizing the mind nourishing heart enhancing memory effect Sialic acid complex polypeptide nutrition element.Product of the present invention has stronger bioactivity, improves human body basic metabolism, can generate active influence to tranquilizing the mind nourishing heart, sleeping intelligence development, enhancing memory effect.
Description
Technical field
The present invention relates to nutrient functional foods and biological fermentation field.It is more particularly related to a kind of tranquilizing the mind
Nourishing heart enhances memory effect Sialic acid complex polypeptide nutrition element and preparation method.
Background technique
With advancing age, amount of exercise is also being reduced the elderly, and the old man to lack exercise, passive protective physical fitness is nothing like
Young man.For a middle-aged person because of huge operating pressure, sleep quality is poor.Lack movement and sleep, will lead to deficiency of heart-QI, the heart
Throb with fear, have a sleepless night, a variety of symptoms such as mind uneasiness.The cooking methods such as the traditional stir-fry in China, pan-fried, fried will lead to the nutrient in food
It is largely lost, is nourished by food and will lead to the burden of masticatory function and digestion function of stomach and intestine.Therefore, the middle-aged and the old is in reasonable meals
On the basis of food, some nutritional supplements are suitably supplemented, improve dietetic nutrition degree, and do not increase the negative of stomach and digestive system
Load improves human body basic metabolism, and various symptoms caused by improving because of age, pressure are necessary.
Summary of the invention
It is an object of the invention to solve at least the above problems, and provide the advantages of at least will be described later.
It is a still further object of the present invention to provide a kind of tranquilizing the mind nourishing heart enhancing memory effect Sialic acid complex polypeptide nutrition elements
And preparation method improves human body basic metabolism with stronger bioactivity, it can be to tranquilizing the mind nourishing heart, sleeping intelligence development, enhancing
Memory effect generates active influence.
In order to realize these purposes and other advantages according to the present invention, a kind of tranquilizing the mind nourishing heart enhancing memory effect is provided
Sialic acid complex polypeptide nutrition element, the raw material including following parts by weight: 15 parts of Sialic acid, 15 parts of γ-aminobutyric acid, semen ziziphi spinosae 10
Part, 15 parts of albumin polypeptide, 15 parts of phosphatidylserine, 15 parts of DHA powder, 15 parts of fermented ginseng powder, 60 parts of fermented whole eggs powder, Fu
10 parts of Siberian cocklebur, 10 parts of pueraria lobata, 10 parts of lotus seeds, 10 parts of cape jasmine, 10 parts of fingered citron, 10 parts of sanchi flower, 10 parts of semen coicis, compound B race dimension life
1 part, 1 part of xylo-oligosaccharide of element.
Preferably, the fermented whole eggs powder the preparation method comprises the following steps:
Step 1: by fresh birds, beasts and eggs raw material after tap water cleans, then through the cleaning of high concentration electrolytic Ozone Water, ejected wash water
Middle ozone concentration is 20mg/L, water flow 300L/h, dries after sterilization, 70% edible alcohol disinfection eggshell surface, 2 times of fowl are added
The edible rice vinegar of egg size amount, the concentration of edible rice vinegar are 20wt.%, flood and impregnate birds, beasts and eggs 3 days and all dissolve to eggshell, filtering
And acid solution is collected, egg film is eluted with deionized water, eggshell insoluble matter and pigment is removed, floods and be immersed in the acid of collection again
In solution；
Step 2: stirring egg body to discharge egg film and egg liquid, continues to hydrolyze under ultrasonic wave auxiliary 12 months to egg pulp
Uniformly without agglomeration, ultrasonic wave acting frequency is 300Hz, and the lipase of 1/500 times of egg weight is added in ultrasonic time 15min
And the protease of 1/300 times of egg weight, continue enzyme hydrolysis 3 hours, obtain hydrolysis egg pulp, wherein lipase derives from rice root
Mould, phosphatidase is phospholipase C, and protease derives from the acid protease of aspergillus niger and bacillus；
Rear homogeneous is stirred evenly Step 3: hydrolysis egg pulp is mixed with 4wt.% brown sugar, 10wt.% inulin prebiotics, is sterilized,
Cooling, inoculation yeast, lactic acid bacteria, bacterium solution inoculum concentration are respectively hydrolyze egg pulp volume 3%, ferment 8 days at 35 DEG C, obtain shell egg
Body fermentation liquid, wherein yeast is saccharomyces cerevisiae, and lactic acid bacteria is lactobacillus acidophilus and Fei Shi Propionibacterium；
Step 4: by shell egg body filtering fermentation liquor, volume mass is added under ultrasonic wave auxiliary than the cyclodextrin for 10%,
Ultrasonic wave acting frequency is 300Hz, ultrasonic time 15min, high speed dispersion, mixing homogeneous, embedding treatment, 0-5 DEG C of low temperature cold
But it stands, centrifugation removing cholesterol insoluble matter, separating liquid freeze-drying obtains fermented whole eggs powder.
Preferably, in step 1, edible rice vinegar is pre-processed by freeze-thaw specifically: edible rice vinegar is placed in -20
DEG C, 0 DEG C of the mixture of ice and water dissolution of 0.5 times of edible rice vinegar weight is added, stirring forms ice crystal, adds 0.5 times of edible rice
0 DEG C of mixture of ice and water of vinegar weight dissolves, and stirring forms ice crystal, is placed in -20 DEG C, freezes 1h, smashs to pieces, with the speed of 1 DEG C/min
Rate is heated to 0 DEG C, and 0 DEG C of mixture of ice and water is added, and the concentration of edible rice vinegar is made to reach 20wt.%, refrigerate 1h to get.
The preparation method of tranquilizing the mind nourishing heart enhancing memory effect Sialic acid complex polypeptide nutrition element, comprising:
Step a, the semen ziziphi spinosae of above-mentioned parts by weight, Poria cocos, pueraria lobata, lotus seeds, cape jasmine, fingered citron, sanchi flower, semen coicis are mixed
It closes, crushes, be added the 70wt.% ethyl alcohol of 5 times of total weights, refluxing extraction 2h, filtering obtains first dregs of a decoction and the first medical fluid, by the
The centrifuge separation of one medical fluid, obtains the first supernatant, and the 80wt.% ethyl alcohol of 4 times of total weights is added into first dregs of a decoction, and reflux mentions
It takes, filters, obtain second dregs of a decoction and the second medical fluid, the second medical fluid is centrifugated, the second supernatant is obtained, add into second dregs of a decoction
Enter the 90wt.% ethyl alcohol of 3 times of total weights, refluxing extraction, filtering obtains the third dregs of a decoction and third medical fluid, by the centrifugation point of third medical fluid
From obtaining third supernatant, merge the first supernatant, the second supernatant, third supernatant, being concentrated under reduced pressure into relative density is
1.5, it dry, pulverize, spray Purple Perilla Seed Oil, oversize is placed in -20 DEG C of environment and freezes 1h, screenings is placed in 0 DEG C by sieving
1h is refrigerated in environment, is mixed again, is sprayed Purple Perilla Seed Oil, be dry, pulverize, obtain Chinese medical extract powder；
Step b, by the Sialic acid of above-mentioned parts by weight, γ-aminobutyric acid, albumin polypeptide, phosphatidylserine, DHA
Powder, fermented ginseng powder, fermented whole eggs powder, composite B vitamin, xylo-oligosaccharide mixing, are added the Chinese medical extract of step a preparation
Powder dry, pulverize, sieving to get.
Tranquilizing the mind nourishing heart enhances memory effect Sialic acid complex polypeptide nutrition element in the product that preparation improves sleep, nourishing heart
Using.
Tranquilizing the mind nourishing heart enhances application of the memory effect Sialic acid complex polypeptide nutrition element in the product of preparation intelligence development.
Tranquilizing the mind nourishing heart enhances memory effect Sialic acid complex polypeptide nutrition element in the production of preparation relax bowel and defecation and adjusting constipation
Application in product.
The present invention is include at least the following beneficial effects:
Product of the invention is directed to middle-aged and the old group, improves dietetic nutrition degree, does not increase stomach and digestive system
Burden improves human body basic metabolism, various symptoms caused by improving because of age, pressure, in selected Chinese medicine preparation concentration gradient
Rise, the alcohol liquid that dosage gradient reduces carries out the multiple alcohol extractings of the dregs of a decoction, can further by cell wall and cellular membrane disruption or dissolution,
Effective ingredient is improved to separate from matrix, compounding adds each nutriment, wherein by sour water solution and dual enzyme hydrolysis and
The methods of fermentation and embedding not only increase the release of shell egg (eggshell, shell membrane, egg white and egg yolk liquid) nutrient, through biodegrade
With absorption rate is improved after conversion, and be stripped of the egg fishy smell of former egg liquid powdered egg and cross high cholesterol, product is rich in more
There is improvement sleep, nourishing heart to pacify for kind organic calcium, phosphatide type polypeptide oligopeptides and endogenous activity enzyme isoreactivity substance, optimization formula preparation
Mind, adjusts the multiple biological activities such as constipation, relax bowel and defecation at intelligence development, can be widely applied to nutrient functional foods, special meals spy cures food
Product, health food etc., can also further separating-purifying related nutritional functional materials, be applied to biomedicine field and food led
In terms of the non-medical usage in domain.
Further advantage, target and feature of the invention will be partially reflected by the following instructions, and part will also be by this
The research and practice of invention and be understood by the person skilled in the art.
Specific embodiment
Below with reference to example, the present invention is described in further detail, to enable those skilled in the art referring to specification text
Word can be implemented accordingly.
It should be appreciated that such as " having ", "comprising" and " comprising " term used herein do not allot one or more
The presence or addition of a other elements or combinations thereof.
It should be noted that test method described in following embodiments is unless otherwise specified conventional method, institute
Reagent and material are stated, unless otherwise specified, is commercially obtained.
<example 1>
Tranquilizing the mind nourishing heart enhances memory effect Sialic acid complex polypeptide nutrition element, the raw material including following parts by weight: Sialic acid
15 parts, 15 parts of γ-aminobutyric acid, 10 parts of semen ziziphi spinosae, 15 parts of albumin polypeptide, 15 parts of phosphatidylserine, 15 parts of DHA powder, people
15 parts of yeast powder of ginseng, 60 parts of fermented whole eggs powder, 10 parts of Poria cocos, 10 parts of pueraria lobata, 10 parts of lotus seeds, 10 parts of cape jasmine, 10 parts of fingered citron, Radix Notoginseng
Spend 10 parts, 10 parts of semen coicis, 1 part of composite B vitamin, 1 part of xylo-oligosaccharide.
Preparation method includes:
Step a, the semen ziziphi spinosae of above-mentioned parts by weight, Poria cocos, pueraria lobata, lotus seeds, cape jasmine, fingered citron, sanchi flower, semen coicis are mixed
It closes, crushes, be added the 70wt.% ethyl alcohol of 5 times of total weights, refluxing extraction 2h, filtering obtains first dregs of a decoction and the first medical fluid, by the
The centrifuge separation of one medical fluid, obtains the first supernatant, and the 80wt.% ethyl alcohol of 4 times of total weights is added into first dregs of a decoction, and reflux mentions
It takes, filters, obtain second dregs of a decoction and the second medical fluid, the second medical fluid is centrifugated, the second supernatant is obtained, add into second dregs of a decoction
Enter the 90wt.% ethyl alcohol of 3 times of total weights, refluxing extraction, filtering obtains the third dregs of a decoction and third medical fluid, by the centrifugation point of third medical fluid
From obtaining third supernatant, merge the first supernatant, the second supernatant, third supernatant, being concentrated under reduced pressure into relative density is
1.5, it dry, pulverize, spray Purple Perilla Seed Oil, oversize is placed in -20 DEG C of environment and freezes 1h, screenings is placed in 0 DEG C by sieving
1h is refrigerated in environment, is mixed again, is sprayed Purple Perilla Seed Oil, be dry, pulverize, obtain Chinese medical extract powder；
Step b, by the Sialic acid of above-mentioned parts by weight, γ-aminobutyric acid, albumin polypeptide, phosphatidylserine, DHA
Powder, fermented ginseng powder, fermented whole eggs powder (taking sale or made products on the market), composite B vitamin, xylo-oligosaccharide mixing,
Be added step a preparation Chinese medical extract powder, dry, pulverize, sieving to get.
The preparation method of fermented ginseng powder: taking fresh ginseng, and 20-30 DEG C of water temperature cleaning is slurried using beater, obtains people
Ginseng slurry；Into ginseng pulp plus water, amount of water are 40 times of volumes of ginseng pulp, cross colloid mill, obtain ginseng liquid；Ginseng liquid is heated to 40
DEG C, zymophyte is added, fermentation bacterium amount, which is behaved, joins 0.05 times of quality of liquid, and zymophyte is Bulgarian Lactobacillus and streptococcus thermophilus
1:1 mass ratio, ferment 10h, decomposes polysaccharose substance, and ginsenoside is precipitated, obtains fermented ginseng stoste；It is freeze-dried to obtain ginseng hair
Ferment powder.
<example 2>
Tranquilizing the mind nourishing heart enhances memory effect Sialic acid complex polypeptide nutrition element, the raw material including following parts by weight: Sialic acid
15 parts, 15 parts of γ-aminobutyric acid, 10 parts of semen ziziphi spinosae, 15 parts of albumin polypeptide, 15 parts of phosphatidylserine, 15 parts of DHA powder, people
15 parts of yeast powder of ginseng, 60 parts of fermented whole eggs powder, 10 parts of Poria cocos, 10 parts of pueraria lobata, 10 parts of lotus seeds, 10 parts of cape jasmine, 10 parts of fingered citron, Radix Notoginseng
Spend 10 parts, 10 parts of semen coicis, 1 part of composite B vitamin, 1 part of xylo-oligosaccharide.
Preparation method includes:
Step a, the semen ziziphi spinosae of above-mentioned parts by weight, Poria cocos, pueraria lobata, lotus seeds, cape jasmine, fingered citron, sanchi flower, semen coicis are mixed
It closes, crushes, be added the 70wt.% ethyl alcohol of 5 times of total weights, refluxing extraction 2h, filtering obtains first dregs of a decoction and the first medical fluid, by the
The centrifuge separation of one medical fluid, obtains the first supernatant, and the 80wt.% ethyl alcohol of 4 times of total weights is added into first dregs of a decoction, and reflux mentions
It takes, filters, obtain second dregs of a decoction and the second medical fluid, the second medical fluid is centrifugated, the second supernatant is obtained, add into second dregs of a decoction
Enter the 90wt.% ethyl alcohol of 3 times of total weights, refluxing extraction, filtering obtains the third dregs of a decoction and third medical fluid, by the centrifugation point of third medical fluid
From obtaining third supernatant, merge the first supernatant, the second supernatant, third supernatant, being concentrated under reduced pressure into relative density is
1.5, it dry, pulverize, spray Purple Perilla Seed Oil, oversize is placed in -20 DEG C of environment and freezes 1h, screenings is placed in 0 DEG C by sieving
1h is refrigerated in environment, is mixed again, is sprayed Purple Perilla Seed Oil, be dry, pulverize, obtain Chinese medical extract powder；
Step b, by the Sialic acid of above-mentioned parts by weight, γ-aminobutyric acid, albumin polypeptide, phosphatidylserine, DHA
Powder, 15 parts of fermented ginseng powder, fermented whole eggs powder, composite B vitamin, xylo-oligosaccharide mixing, the Chinese medicine that step a preparation is added mention
Take object powder, dry, pulverize, sieving to get.
Fermented whole eggs powder the preparation method comprises the following steps:
Step 1: by fresh birds, beasts and eggs raw material after tap water cleans, then through the cleaning of high concentration electrolytic Ozone Water, ejected wash water
Middle ozone concentration is 20mg/L, water flow 300L/h, dries after sterilization, 70% edible alcohol disinfection eggshell surface, 2 times of fowl are added
The edible rice vinegar of egg size amount, the concentration of edible rice vinegar are 20wt.%, flood and impregnate birds, beasts and eggs 3 days and all dissolve to eggshell, filtering
And acid solution is collected, egg film is eluted with deionized water, eggshell insoluble matter and pigment is removed, floods and be immersed in the acid of collection again
In solution, wherein edible rice vinegar is pre-processed by freeze-thaw specifically: edible rice vinegar is placed in -20 DEG C, 0.5 times of food is added
It is dissolved with 0 DEG C of mixture of ice and water of rice vinegar weight, stirring forms ice crystal, adds 0 DEG C of 0.5 times of edible rice vinegar weight
Mixture of ice and water dissolution, stirring form ice crystal, are placed in -20 DEG C, freeze 1h, smash to pieces, be heated to 0 DEG C with the rate of 1 DEG C/min,
0 DEG C of mixture of ice and water is added, the concentration of edible rice vinegar is made to reach 20wt.%, refrigerate 1h to get；
Step 2: stirring egg body to discharge egg film and egg liquid, continues to hydrolyze under ultrasonic wave auxiliary 12 months to egg pulp
Uniformly without agglomeration, ultrasonic wave acting frequency is 300Hz, and the lipase of 1/500 times of egg weight is added in ultrasonic time 15min
And the protease of 1/300 times of egg weight, continue enzyme hydrolysis 3 hours, obtain hydrolysis egg pulp, wherein lipase derives from rice root
Mould, phosphatidase is phospholipase C, and protease derives from the acid protease of aspergillus niger and bacillus；
Rear homogeneous is stirred evenly Step 3: hydrolysis egg pulp is mixed with 4wt.% brown sugar, 10wt.% inulin prebiotics, is sterilized,
Cooling, inoculation yeast, lactic acid bacteria, bacterium solution inoculum concentration are respectively hydrolyze egg pulp volume 3%, ferment 8 days at 35 DEG C, obtain shell egg
Body fermentation liquid, wherein yeast is saccharomyces cerevisiae, and lactic acid bacteria is lactobacillus acidophilus and Fei Shi Propionibacterium；
Step 4: by shell egg body filtering fermentation liquor, volume mass is added under ultrasonic wave auxiliary than the cyclodextrin for 10%,
Ultrasonic wave acting frequency is 300Hz, ultrasonic time 15min, high speed dispersion, mixing homogeneous, embedding treatment, 0-5 DEG C of low temperature cold
But it stands, centrifugation removing cholesterol insoluble matter, separating liquid freeze-drying obtains fermented whole eggs powder.
The preparation method of fermented ginseng powder: taking fresh ginseng, and 20-30 DEG C of water temperature cleaning is slurried using beater, obtains people
Ginseng slurry；Into ginseng pulp plus water, amount of water are 40 times of volumes of ginseng pulp, cross colloid mill, obtain ginseng liquid；Ginseng liquid is heated to 40
DEG C, zymophyte is added, fermentation bacterium amount, which is behaved, joins 0.05 times of quality of liquid, and zymophyte is Bulgarian Lactobacillus and streptococcus thermophilus
1:1 mass ratio, ferment 10h, decomposes polysaccharose substance, and ginsenoside is precipitated, obtains fermented ginseng stoste；It is freeze-dried to obtain ginseng hair
Ferment powder.
<the pretreatment efficacy assessments of Chinese medicine material are tested>
By Escherichia coli (Escherichia coli), salmonella typhimurium (Salmonella typhimurium),
Salmonella choleraesuls (Salmonella choleraesuis), Salmonella gallinarum (Salmonella gullinarum), gold
Staphylococcus aureus (Staphylococcus aureus) is inoculated on agar slant culture-medium, and 37 DEG C of cultures make its work for 24 hours
Change, is inoculated in the sterile fresh meat soup of 2mL, 37 DEG C of culture 18h are configured to 10 through turbidimetry for Determination viable bacteria6The bacterium solution of CFU/mL
It is spare.The water constant volume for taking the product of example 1, comparative example 1, comparative example 2 to be measured with 1 times carries out external drug to the above strains tested
Sensitization test measures inhibition zone size.
<comparative example 1>
Tranquilizing the mind nourishing heart enhances memory effect Sialic acid complex polypeptide nutrition element, the raw material including following parts by weight: Sialic acid
15 parts, 15 parts of γ-aminobutyric acid, 10 parts of semen ziziphi spinosae, 15 parts of albumin polypeptide, 15 parts of phosphatidylserine, 15 parts of DHA powder, people
15 parts of yeast powder of ginseng, 60 parts of fermented whole eggs powder, 10 parts of Poria cocos, 10 parts of pueraria lobata, 10 parts of lotus seeds, 10 parts of cape jasmine, 10 parts of fingered citron, Radix Notoginseng
Spend 10 parts, 10 parts of semen coicis, 1 part of composite B vitamin, 1 part of xylo-oligosaccharide.
Preparation method includes:
Step a, the semen ziziphi spinosae of above-mentioned parts by weight, Poria cocos, pueraria lobata, lotus seeds, cape jasmine, fingered citron, sanchi flower, semen coicis are mixed
Conjunction crushes, and 80% alcohol solution dipping 1h is added, and flow back 1h, power, and 80% ethanol solution reflux 1h is added, and merging filtrate steams
It evaporates and is recycled to ethyl alcohol and is evaporated, dry, pulverize, obtain Chinese medical extract powder；
Step b, by the Sialic acid of above-mentioned parts by weight, γ-aminobutyric acid, albumin polypeptide, phosphatidylserine, DHA
Powder, fermented ginseng powder, fermented whole eggs powder (taking sale or made products on the market), composite B vitamin, xylo-oligosaccharide mixing,
Be added step a preparation Chinese medical extract powder, dry, pulverize, sieving to get.
The preparation method of fermented ginseng powder: taking fresh ginseng, and 20-30 DEG C of water temperature cleaning is slurried using beater, obtains people
Ginseng slurry；Into ginseng pulp plus water, amount of water are 40 times of volumes of ginseng pulp, cross colloid mill, obtain ginseng liquid；Ginseng liquid is heated to 40
DEG C, zymophyte is added, fermentation bacterium amount, which is behaved, joins 0.05 times of quality of liquid, and zymophyte is Bulgarian Lactobacillus and streptococcus thermophilus
1:1 mass ratio, ferment 10h, decomposes polysaccharose substance, and ginsenoside is precipitated, obtains fermented ginseng stoste；It is freeze-dried to obtain ginseng hair
Ferment powder.
<comparative example 2>
Tranquilizing the mind nourishing heart enhances memory effect Sialic acid complex polypeptide nutrition element, the raw material including following parts by weight: Sialic acid
15 parts, 15 parts of γ-aminobutyric acid, 10 parts of semen ziziphi spinosae, 15 parts of albumin polypeptide, 15 parts of phosphatidylserine, 15 parts of DHA powder, people
15 parts of yeast powder of ginseng, 60 parts of fermented whole eggs powder, 10 parts of Poria cocos, 10 parts of pueraria lobata, 10 parts of lotus seeds, 10 parts of cape jasmine, 10 parts of fingered citron, Radix Notoginseng
Spend 10 parts, 10 parts of semen coicis, 1 part of composite B vitamin, 1 part of xylo-oligosaccharide.
Preparation method includes:
Step a, the semen ziziphi spinosae of above-mentioned parts by weight, Poria cocos, pueraria lobata, lotus seeds, cape jasmine, fingered citron, sanchi flower, semen coicis are mixed
It closes, crushes, add distilled water immersion, cooling with moderate heat microwave treatment 5min, repetition microwave treatment is primary, and centrifugation takes supernatant,
Water-bath concentration, dry, pulverize, obtain Chinese medical extract powder；
Step b, by the Sialic acid of above-mentioned parts by weight, γ-aminobutyric acid, albumin polypeptide, phosphatidylserine, DHA
Powder, fermented ginseng powder, fermented whole eggs powder (taking sale or made products on the market), composite B vitamin, xylo-oligosaccharide mixing,
Be added step a preparation Chinese medical extract powder, dry, pulverize, sieving to get.
The preparation method of fermented ginseng powder: taking fresh ginseng, and 20-30 DEG C of water temperature cleaning is slurried using beater, obtains people
Ginseng slurry；Into ginseng pulp plus water, amount of water are 40 times of volumes of ginseng pulp, cross colloid mill, obtain ginseng liquid；Ginseng liquid is heated to 40
DEG C, zymophyte is added, fermentation bacterium amount, which is behaved, joins 0.05 times of quality of liquid, and zymophyte is Bulgarian Lactobacillus and streptococcus thermophilus
1:1 mass ratio, ferment 10h, decomposes polysaccharose substance, and ginsenoside is precipitated, obtains fermented ginseng stoste；It is freeze-dried to obtain ginseng hair
Ferment powder.
Table 1
Test result shows that the fungistatic effect of example 1 is promoted relative to comparative example 1, comparative example 2, this is because with
The alcohol liquid that concentration gradient rises, dosage gradient reduces carries out the multiple alcohol extracting of the dregs of a decoction, can be further by cell wall and cell membrane
Broken or dissolution improves effective ingredient and separates from matrix.
<the pretreatment efficacy assessments of edible rice vinegar are tested>
1, influence of the pretreatment of edible rice vinegar to the extraction of egg film keratin
Bird egg is dried, the edible rice vinegar of 2 times of birds, beasts and eggs weight is added, the concentration of edible rice vinegar is 20wt.%, is flooded
And impregnate birds, beasts and eggs 3 days and all dissolved to eggshell, acid solution is filtered and collected, egg film is eluted with water, removes eggshell insoluble matter and color
Element removes yolk and egg white, leaves egg film, cleans, dry, and weighing obtains egg film initial mass, floods again and be immersed in collection
Acid solution in.
It is just completely dissolved with egg film, is terminal without the apparent fragment of naked eyes, calculates egg film dissolution time, solution is existed
It is concentrated into 100mL down at room temperature, adjusting pH separates keratin precipitation to isoelectric point, stands 30min, centrifuge separation will be insoluble
Object mentions 1 time again, and lower layer's keratin is transferred to diameter 25mm, molecular cut off 8000-14 000D, regenerated cellulose material and is dialysed
Bag dialysis 3d, every 4h change 1 water.After the completion of dialysis, by purified keratin in -45 DEG C of vacuum pressure 15Pa, temperature conditions
Lower vacuum freeze drying, obtains keratin, and weighing obtains keratin quality, at the beginning of calculating keratin yield=keratin quality/egg film
Prothyl amount × 100%.
<comparative example 3>
Edible rice vinegar is edible rice vinegar at room temperature.
<comparative example 4>
Edible rice vinegar be by the pretreated edible rice vinegar of freeze-thaw, specifically: edible rice vinegar is placed in -20 DEG C, is added
Enter 0 DEG C of the mixture of ice and water dissolution of 0.5 times of edible rice vinegar weight, stirring forms ice crystal, adds 0.5 times of edible rice vinegar weight
0 DEG C of the mixture of ice and water dissolution of amount, stirring are formed ice crystal, are placed in -20 DEG C, freezed 1h, smash to pieces, added with the rate of 1 DEG C/min
0 DEG C of mixture of ice and water is added to 0 DEG C in heat, and the concentration of edible rice vinegar is made to reach 20wt.%, refrigerate 1h to get.
Table 2
Test result shows the egg film dissolution time of comparative example 4 considerably less than comparative example 3, and keratin yield is significantly high
Egg film can be swollen with faster speed, be broken this is because edible rice vinegar is to pre-process by freeze-thaw in comparative example 3
The hydrogen bond and salt type bond on bad egg's film surface layer discharge the keratin being embedded in egg film.
2, the influence that the amino acid of albumen is extracted in the pretreatment of edible rice vinegar
Bird egg is dried, the edible rice vinegar of 2 times of birds, beasts and eggs weight is added, the concentration of edible rice vinegar is 20wt.%, is flooded
And impregnate birds, beasts and eggs 3 days and all dissolved to eggshell, acid solution is filtered and collected, egg film is eluted with water, removes eggshell insoluble matter and color
Element, in the acid solution for flooding and being immersed in again collection；
Egg body is stirred to discharge egg film and egg liquid, continues hydrolysis 12 months to egg pulp uniformly without knot under ultrasonic wave auxiliary
Block, ultrasonic wave acting frequency are 300Hz, ultrasonic time 15min, and the protease of 1/300 times of egg weight is added, continues enzyme water
Solution 3 hours, obtains hydrolysis egg pulp, wherein lipase derives from Rhizopus oryzae, and phosphatidase is phospholipase C, and protease derives from black song
Mould and bacillus acid protease；
Hydrolysis egg pulp is mixed with 4wt.% brown sugar, 10wt.% inulin prebiotics and stirs evenly rear homogeneous, is sterilized, it is cooling, it connects
Primary yeast, lactic acid bacteria, bacterium solution inoculum concentration are respectively hydrolyze egg pulp volume 3%, are fermented 8 days at 35 DEG C, and the fermentation of shell egg body is obtained
Liquid, wherein yeast is saccharomyces cerevisiae, and lactic acid bacteria is lactobacillus acidophilus and Fei Shi Propionibacterium, by shell egg body filtering fermentation liquor,
Matter.Amino acid content mg/100mL is measured using GB/T 5009.124-2003 method.
<comparative example 5>
Edible rice vinegar is edible rice vinegar at room temperature.
<comparative example 6>
Edible rice vinegar be by the pretreated edible rice vinegar of freeze-thaw, specifically: edible rice vinegar is placed in -20 DEG C, is added
Enter 0 DEG C of the mixture of ice and water dissolution of 0.5 times of edible rice vinegar weight, stirring forms ice crystal, adds 0.5 times of edible rice vinegar weight
0 DEG C of the mixture of ice and water dissolution of amount, stirring are formed ice crystal, are placed in -20 DEG C, freezed 1h, smash to pieces, added with the rate of 1 DEG C/min
0 DEG C of mixture of ice and water is added to 0 DEG C in heat, and the concentration of edible rice vinegar is made to reach 20wt.%, refrigerate 1h to get.
Table 3
Test result shows that comparative example 6 detects 18 kinds of amino acid, and comparative example 5 detects 14 kinds of amino acid, this is because
Edible rice rice vinegar is pre-processed by freeze-thaw, being capable of keratin release in egg film, enhancing bioactivity and biological utilisation
Degree.
<test of complex polypeptide nutrition element efficacy assessments>
1, nourishing heart is tested
Select male mouse of kunming 50 of 18-22g, raising after a week, be randomly divided into blank control group, low dose group,
Middle dose group, high dose group, preferably middle dose group, every group 10.
It is prepared by the isometric distilled water of blank control group stomach-filling, high dose group, middle dose group, low dose group stomach-filling example 1
Complex polypeptide nutrition element, dosage is respectively 300mg/ (kgd), 150mg/ (kgd), 20mg/ (kgd), and each group is daily
Stomach-filling 1 time, continuous processing 4 weeks.It is preferred that complex polypeptide nutrition element prepared by middle dose group stomach-filling example 2, dosage 150mg/
(kgd), according to each group dosage, with 0.1mL10g-114d is administered in volume, continuous gavage.Fasting can't help before last dose
Mouse is put into the mouse autonomic activities analyzer for having been turned on power supply after 50min is administered, adapts to 5min, then measure simultaneously by water 12h
Record influence of the mouse autonomic activities number to mouse autonomic activities in 5min.
Table 4
Test result shows that high dose group, middle dose group, low dose group, preferably middle dose group are small with respect to blank control group
Mouse autonomic activities number significantly reduces, this is because the application can substantially reduce mouse autonomic activities number, has calm nourishing heart
Effect.
2, improve sleep laboratory
Male mouse of kunming 50 for selecting 18-22g, raising after a week, are randomly divided into blank control group, ethyl alcohol extracts
Group, Microwave Extraction group, complex polypeptide nutrition element group, preferably complex polypeptide nutrition element group, every group 10.
The distilled water of blank control group stomach-filling 150mg/ (kgd), product prepared by ethyl alcohol extraction group stomach-filling comparative example 1,
Dosage is 150mg/ (kgd), and product prepared by Microwave Extraction group stomach-filling comparative example 2, dosage is 150mg/ (kgd), compound
Complex polypeptide nutrition element prepared by polypeptide nutrition element group stomach-filling example 1, dosage is respectively 150mg/ (kgd), preferably compound more
Peptide nutrient group stomach-filling example 2 prepare preferred complex polypeptide nutrition element, dosage be 150mg/ (kgd), daily stomach-filling 1 time,
Continuous gavage 30d.
2.1 barbital sodium sub-threshold dose hypnosis experiments and Sleep Latency Test
After last stomach-filling 30min, barbital sodium is injected intraperitoneally by sub-threshold dose 300mg/ (kgBW), injection volume is
0.1mL/ (10gBW), influence of the observation tested material to the mouse sleep rate of barbital sodium under injection threshold, last stomach-filling 30min
Afterwards, barbital sodium is injected intraperitoneally by 300mg/ (kgBW), injection volume is 0.1mL/ (10gBW), and observation tested material is to a bar ratio
Appropriate sodium is on the preclinical influence of mice sleep.The results are shown in Table 5:
Table 5
Test result analysis: complex polypeptide nutrition element group, preferably complex polypeptide nutrition element group are compared with blank control group, second
Alcohol extracting group and Microwave Extraction group compare, and can significantly improve the sleep state of test mice, increase injection sub-threshold dose barbital
The sleep rate of sodium mouse is significant to shorten injection sub-threshold dose barbital sodium mice sleep incubation period.
The test of 2.2 yellow Jackets inducing mouse sleeping times
After last stomach-filling 30min, 300mg/ (kgd) yellow Jackets are injected intraperitoneally, injection volume is 0.1mL/ (10g
BW), using righting reflex loss as index, sleep quantity and sleeping time are recorded, the results are shown in Table 6.
Table 6
Test result analysis: complex polypeptide nutrition element group, preferably complex polypeptide nutrition element group, ethyl alcohol extraction group, microwave mention
Take group compared with blank control group, sleeping time significantly extends, and rate elongation is up to 106.4%；Complex polypeptide nutrition element group, preferably
Compared with ethyl alcohol extraction group and Microwave Extraction group, mouse sleep time significantly extends complex polypeptide nutrition element group.
2.3 personnel observation
40 people with sleep disturbance are chosen, are randomly divided into blank control group, complex polypeptide nutrition element group, every group 20
People.
Blank control group does not take the drug of any treatment insomnia, and complex polypeptide nutrition element group takes the production of the preparation of example 1
Product, dosage 5.5g/d take 1 time daily, continuously take 60d.
In 90d, Pittsburgh Sleep Quality Index evaluation table is filled in, the results are shown in Table 7.
Table 7
Remarks: grading system: 0-5 points, sleep quality is fine；6-10 points, sleep quality can manage it；11-15 points, sleep quality
Generally；16-21 points, sleep quality is very poor.
Analysis of experimental results: compared with blank control group, sleep quality significantly improves complex polypeptide nutrition element group.
3, intelligence development is tested
3.1 keep away dark test
Male mouse of kunming 50 for selecting 18-22g, raising after a week, are randomly divided into blank control group, ethyl alcohol extracts
Group, Microwave Extraction group, complex polypeptide nutrition element group, preferably complex polypeptide nutrition element group, every group 10.
The distilled water of blank control group stomach-filling 150mg/ (kgd), product prepared by ethyl alcohol extraction group stomach-filling comparative example 1,
Dosage is 150mg/ (kgd), and product prepared by Microwave Extraction group stomach-filling comparative example 2, dosage is 150mg/ (kgd), compound
Complex polypeptide nutrition element prepared by polypeptide nutrition element group stomach-filling example 1, dosage is respectively 150mg/ (kgd), preferably compound more
Peptide nutrient group stomach-filling example 2 prepare preferred complex polypeptide nutrition element, dosage be 150mg/ (kgd), daily stomach-filling 1 time,
Continuous gavage 30d, 31d start to train, and continue during training with identical dosage stomach-filling, 1 time a day, by animal face when test
Portion is put into bright room backwards to hole, while starting timer, and animal enters darkroom across hole and shocked by electricity, and timer stops automatically
Only, mouse is taken out, every animal is recorded and enters the time required to darkroom keeps away electric shock from bright room, this i.e. incubation period.It weighs for 24 hours or after 48h
It is tested, records every animal 10min and enter darkroom number, i.e. errors number, the results are shown in Table 8.
Table 8
Test result analysis: complex polypeptide nutrition element group, preferably complex polypeptide nutrition element group, ethyl alcohol extraction group, microwave mention
Take group compared with blank control group, into darkroom number, i.e. mouse wrong times significantly reduce；
Complex polypeptide nutrition element group, preferably complex polypeptide nutrition element group are small compared with ethyl alcohol extraction group and Microwave Extraction group
Mouse errors number substantially reduces.
3.2 water maze test
Male mouse of kunming 50 for selecting 18-22g, raising after a week, are randomly divided into blank control group, ethyl alcohol extracts
Group, Microwave Extraction group, complex polypeptide nutrition element group, preferably complex polypeptide nutrition element group, every group 10.
The distilled water of blank control group stomach-filling 150mg/ (kgd), product prepared by ethyl alcohol extraction group stomach-filling comparative example 1,
Dosage is 150mg/ (kgd), and product prepared by Microwave Extraction group stomach-filling comparative example 2, dosage is 150mg/ (kgd), compound
Complex polypeptide nutrition element prepared by polypeptide nutrition element group stomach-filling example 1, dosage is respectively 150mg/ (kgd), preferably compound more
Peptide nutrient group stomach-filling example 2 prepare preferred complex polypeptide nutrition element, dosage be 150mg/ (kgd), daily stomach-filling 1 time,
Continuous gavage 30d, 31d start to train, and continue with identical dosage stomach-filling, 1 time a day during training.The labyrinth swimming lane depth of water
15cm, 25 DEG C of water temperature, the time is limited to 120s, and the animal for not arriving terminal in 120s is denoted as 120s.When test, animal is put
In swimming lane starting point, while start key is pressed, record animal is reached home time and the number of animals reached home, as a result such as table 9
It is shown.
Table 9
Test result analysis:
Complex polypeptide nutrition element group, preferably complex polypeptide nutrition element group, ethyl alcohol extraction group, Microwave Extraction group and blank control
Group compares, and mouse goes out the labyrinth time and significantly reduces；
Complex polypeptide nutrition element group, preferably complex polypeptide nutrition element group are small compared with ethyl alcohol extraction group and Microwave Extraction group
Mouse goes out the labyrinth time and substantially reduces.
4, relax bowel and defecation adjusts constipation test
Male mouse of kunming 60 for selecting 18-22g, raising after a week, divide blank control group, model group, ethyl alcohol at random
Extraction group, Microwave Extraction group, complex polypeptide nutrition element group, preferably complex polypeptide nutrition element group, every group 10.
Blank control group, model group feed normal diet, and ethyl alcohol extraction group feeds the general of the product prepared containing comparative example 1
Logical feed, dosage are 150mg/ (kgd), the normal diet for the product that the feeding of Microwave Extraction group is prepared containing comparative example 2, dosage
For 150mg/ (kgd), complex polypeptide nutrition element group feeds the normal diet of the complex polypeptide nutrition element prepared containing example 1,
Dosage is 150mg/ (kgd), and preferably complex polypeptide nutrition element group feeds the preferred complex polypeptide nutrition element prepared containing example 2
Normal diet, dosage be 150mg/ (kgd), continuously raise 15d, observe mouse small intestine push test and defecation test.
The test of 4.1 small intestine push
After 15d is fed, fasting 16h, each group takes 5 mouse, and model group, Microwave Extraction group, is answered at ethyl alcohol extraction group
Close polypeptide nutrition element group, preferably complex polypeptide nutrition element group stomach-filling 5mg/ (kgd) compound ground phenol promise vinegar solution 20mL/ (kg
D), blank control group gives equal amount of distilled water, ethyl alcohol extraction group, Microwave Extraction group, complex polypeptide nutrition element group, excellent after 30min
Complex polypeptide nutrition element group is selected once to give the prepared Chinese ink 10mL/kg containing corresponding product, the mixing of 1:1 in mass ratio, blank pair respectively
Equivalent prepared Chinese ink is given according to group and model group.Cervical dislocation puts to death animal after 25min, opens abdominal cavity immediately and separates mesenterium, clip
Upper end is not added traction and pulls into straight line, measure from the intestinal tube of pylorus, lower end to ileocecus.Length of intestine is " small intestine overall length
Degree " is " prepared Chinese ink propulsion length " from pylorus to prepared Chinese ink forward position.As a result as indicated by 10, ink progradation is calculated according to the following formula.
Ink progradation (P)=prepared Chinese ink promotes length/intestinal tube total length × 100%
Table 10
Test result analysis: as shown in Table 10, compared with blank control, the prepared Chinese ink advance distance and prepared Chinese ink of model group are promoted
Rate is substantially less than blank control group, illustrates modeling success；
Compared with blank control, complex polypeptide nutrition element group, preferably complex polypeptide nutrition element group mouse prepared Chinese ink promote
Rate is above blank control group, compared with ethyl alcohol extraction group, Microwave Extraction group, complex polypeptide nutrition element group, preferably complex polypeptide
The ink progradation of the mouse of nutrient group is above blank control group, illustrates that hair has the function of promoting intestinal motility.
The test of 4.2 defecations
After 15d is fed, fasting 16h, each group takes 5 mouse, and model group, Microwave Extraction group, is answered at ethyl alcohol extraction group
Polypeptide nutrition element group, preferably complex polypeptide nutrition element group stomach-filling 10mg/ (kgd) compound ground phenol promise vinegar solution 20mL/kg are closed, it is empty
White control group gives equal amount of distilled water, ethyl alcohol extraction group, Microwave Extraction group, complex polypeptide nutrition element group, preferably multiple after 30min
It closes polypeptide nutrition element group and once gives the prepared Chinese ink 10mL/kg containing corresponding product, the mixing of 1:1 in mass ratio, blank control group respectively
Equivalent prepared Chinese ink is given with model group, starts simultaneously at timing.Every mouse is individually raised, and supplies feed and drinking-water, observation note
It records every animal and arranges the interior row's melena grain number of melena required time, 6h and weight for the first time.As a result as shown in table 11.
Table 11
Test result analysis: as shown in Table 11, compared with blank control group, it is significant that model group mouse arranges the first grain melena time
It is longer than blank control group, fecal grains and the prosperous substantially less than blank control group of excrement weight, illustrates Constipation Model success.
Complex polypeptide nutrition element group, preferably complex polypeptide nutrition element group, ethyl alcohol extraction group, Microwave Extraction group and model comparison
Group is compared, and complex polypeptide nutrition element group, preferably complex polypeptide nutrition element group are compared with ethyl alcohol extraction group, Microwave Extraction group, first grain
Row's melena time is obviously shortened, and fecal grains and weight dramatically increase, and illustrate have the function of decorporation just.
Number of devices and treatment scale described herein are for simplifying explanation of the invention.To application of the invention,
Modifications and variations will be readily apparent to persons skilled in the art.
Although the embodiments of the present invention have been disclosed as above, but its is not only in the description and the implementation listed
With it can be fully applied to various fields suitable for the present invention, for those skilled in the art, can be easily
Realize other modification, therefore without departing from the general concept defined in the claims and the equivalent scope, the present invention is simultaneously unlimited
In specific details and example shown and described herein.
Claims (7)
1. tranquilizing the mind nourishing heart enhances memory effect Sialic acid complex polypeptide nutrition element, which is characterized in that the original including following parts by weight
Material: 15 parts of Sialic acid, 15 parts of γ-aminobutyric acid, 10 parts of semen ziziphi spinosae, 15 parts of albumin polypeptide, 15 parts of phosphatidylserine, DHA
15 parts of powder, 15 parts of fermented ginseng powder, 60 parts of fermented whole eggs powder, 10 parts of Poria cocos, 10 parts of pueraria lobata, 10 parts of lotus seeds, 10 parts of cape jasmine, fingered citron
10 parts, 10 parts of sanchi flower, 10 parts of semen coicis, 1 part of composite B vitamin, 1 part of xylo-oligosaccharide.
2. tranquilizing the mind nourishing heart enhancing memory effect Sialic acid complex polypeptide nutrition element as described in claim 1, which is characterized in that institute
State fermented whole eggs powder the preparation method comprises the following steps:
Step 1: by fresh birds, beasts and eggs raw material after tap water cleans, then clean through high concentration electrolytic Ozone Water, it is smelly in ejected wash water
Oxygen concentration is 20mg/L, water flow 300L/h, dries after sterilization, 70% edible alcohol disinfection eggshell surface, 2 times of birds, beasts and eggs weights are added
The edible rice vinegar of amount, the concentration of edible rice vinegar are 20wt.%, flood and impregnate birds, beasts and eggs 3 days and all dissolve to eggshell, filter and receive
Collect acid solution, elutes egg film with deionized water, remove eggshell insoluble matter and pigment, flood and be immersed in the acid solution of collection again
In；
Step 2: stirring egg body to discharge egg film and egg liquid, ultrasonic wave auxiliary under continue hydrolysis 12 months it is uniform to egg pulp
Without agglomeration, ultrasonic wave acting frequency is 300Hz, ultrasonic time 15min, and the lipase and 1/ of 1/500 times of egg weight is added
The protease of 300 times of egg weights continues enzyme hydrolysis 3 hours, obtains hydrolysis egg pulp, wherein lipase derives from Rhizopus oryzae, phosphorus
Lipase is phospholipase C, and protease derives from the acid protease of aspergillus niger and bacillus；
Rear homogeneous is stirred evenly Step 3: hydrolysis egg pulp is mixed with 4wt.% brown sugar, 10wt.% inulin prebiotics, is sterilized, it is cold
But, inoculation yeast, lactic acid bacteria, bacterium solution inoculum concentration are respectively hydrolyze egg pulp volume 3%, ferment 8 days at 35 DEG C, obtain shell egg body
Fermentation liquid, wherein yeast is saccharomyces cerevisiae, and lactic acid bacteria is lactobacillus acidophilus and Fei Shi Propionibacterium；
Step 4: volume mass is added than the cyclodextrin for 10%, ultrasound in shell egg body filtering fermentation liquor under ultrasonic wave auxiliary
Wave acting frequency is 300Hz, ultrasonic time 15min, and high speed dispersion mixes homogeneous, and embedding treatment, 0-5 DEG C of sub-cooled be quiet
It sets, centrifugation removing cholesterol insoluble matter, separating liquid freeze-drying obtains fermented whole eggs powder.
3. tranquilizing the mind nourishing heart enhancing memory effect Sialic acid complex polypeptide nutrition element as claimed in claim 2, which is characterized in that step
In rapid one, edible rice vinegar is pre-processed by freeze-thaw specifically: edible rice vinegar is placed in -20 DEG C, 0.5 times of edible rice is added
0 DEG C of mixture of ice and water of vinegar weight dissolves, and stirring forms ice crystal, adds 0 DEG C of ice water of 0.5 times of edible rice vinegar weight
Mixture dissolution, stirring form ice crystal, are placed in -20 DEG C, freeze 1h, smash to pieces, are heated to 0 DEG C with the rate of 1 DEG C/min, are added 0
DEG C mixture of ice and water, so that the concentration of edible rice vinegar is reached 20wt.%, refrigerate 1h to get.
4. the preparation of tranquilizing the mind nourishing heart enhancing memory effect Sialic acid complex polypeptide nutrition element as described in any one of claims 1-3
Method characterized by comprising
Step a, the semen ziziphi spinosae of above-mentioned parts by weight, Poria cocos, pueraria lobata, lotus seeds, cape jasmine, fingered citron, sanchi flower, semen coicis are mixed,
It crushes, the 70wt.% ethyl alcohol of 5 times of total weights, refluxing extraction 2h is added, filtering obtains first dregs of a decoction and the first medical fluid, by the first medicine
Liquid centrifuge separation, obtains the first supernatant, and the 80wt.% ethyl alcohol of 4 times of total weights, refluxing extraction, mistake are added into first dregs of a decoction
Filter, obtains second dregs of a decoction and the second medical fluid, and the second medical fluid is centrifugated, the second supernatant is obtained, and 3 times are added into second dregs of a decoction
The 90wt.% ethyl alcohol of total weight, refluxing extraction, filtering obtain the third dregs of a decoction and third medical fluid, third medical fluid are centrifugated, is obtained
To third supernatant, merge the first supernatant, the second supernatant, third supernatant, being concentrated under reduced pressure into relative density is 1.5, is done
It is dry, it crushes, sprays Purple Perilla Seed Oil, oversize is placed in -20 DEG C of environment and freezes 1h, screenings is placed in 0 DEG C of environment by sieving
1h is refrigerated, is mixed again, Purple Perilla Seed Oil is sprayed, dry, pulverize, obtain Chinese medical extract powder；
Step b, by the Sialic acid of above-mentioned parts by weight, γ-aminobutyric acid, albumin polypeptide, phosphatidylserine, DHA powder, people
Join yeast powder, fermented whole eggs powder, composite B vitamin, xylo-oligosaccharide mixing, the Chinese medical extract powder of step a preparation be added,
Dry, pulverize, sieving to get.
5. prepared by tranquilizing the mind nourishing heart enhancing memory effect Sialic acid complex polypeptide nutrition element as described in any one of claims 1-3
Improve the application in the product of sleep, nourishing heart.
6. prepared by tranquilizing the mind nourishing heart enhancing memory effect Sialic acid complex polypeptide nutrition element as described in any one of claims 1-3
Application in the product of intelligence development.
7. prepared by tranquilizing the mind nourishing heart enhancing memory effect Sialic acid complex polypeptide nutrition element as described in any one of claims 1-3
Application in the product of relax bowel and defecation and adjusting constipation.
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Application publication date: 20190510 |
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