CN105561099A - Traditional Chinese medicine composition for controlling litopenaeus-vannamei enterohepatic sporozoosis and control method thereof - Google Patents
Traditional Chinese medicine composition for controlling litopenaeus-vannamei enterohepatic sporozoosis and control method thereof Download PDFInfo
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Abstract
The invention belongs to the field of seafood breeding, and provides a traditional Chinese medicine composition. The traditional Chinese medicine composition aims at solving the technical problem that enterohepatic sporozoosis is difficult to prevent and cure in the litopenaeus-vannamei breeding process at present. The traditional Chinese medicine composition comprises, by weight, 10-20 parts of melia azedarach, 10-20 parts of cyrtomium rhizomes, 5-15 parts of sessile stemona roots, 20-30 parts of stone-like omphalia, 15-25 parts of seeds of feather cockscomb, 20-30 parts of gelsemium elegans benth and 15-25 parts of betel nuts in a combined mode. A feeding method includes the step that the raw medicinal materials are pulverized, mixed and then mixed into baits to be fed, wherein the weight of the medicine is 1%o-10%o of the weight of the baits. The traditional Chinese medicine composition is free of toxic and side effects on growth of litopenaeus vannamei, and after the traditional Chinese medicine composition is applied, the growth speed of litopenaeus vannamei is high, the yield is high, and economic benefits are remarkable; the traditional Chinese medicine composition is a Chinese herbal medicine low in cost, obvious in control effect and capable of being fed through carriers.
Description
Technical field
The invention belongs to culturing marine products field, specifically refer to a kind of Chinese medicine composition and the prevention and controls thereof of preventing and treating Penaeus vannamei liver sausage sporidiosis.
Background technology
Penaeus vannamei is one of three large shrimps that world today's cultured output is the highest.Penaeus vannamei originates in marine site, South America pacific rim, Institute of Oceanology of the Chinese Academy of Sciences professor Zhang Weiquan takes the lead in introducing this shrimp by the U.S., and nursery pass was breached in 1992, until promote cultivation throughout the country from lab scale to pilot scale, the coastal state such as current China Jiangsu, Guangdong, Guangxi, Fujian, Hainan, Zhejiang, Shandong, Hebei or autonomous region progressively form breeding scale.EHP propagated in the culture of Penaeus vannamei of China in recent years becomes accomplished fact, and becomes severely afflicated area.After Penaeus vannamei infects liver sausage sporozoon; most obvious characteristic is that the speed of growth can become slowly; prawn individual variation is large; cultured prawn appetite is not normally but grown up little; cultivate 60 days individualities and just reach 130/jin; cultivation producer can be used in all time and efforts and thisly with it basic childlike prawn, can not get effectively gathering in the crops and causing tremendous economic to lose, the benign development hindering culture of Penaeus vannamei industry of prawn EHP disease because of ill.The main method of current prevention and control liver sausage sporidiosis has: (1) reduces with the iodine or formalin that are applicable to concentration the risk that EHP is attached to ovum or nauplius in prawn seed-rearing process.(2) use at the bottom of the highly basic disinfecting substance process such as quicklime, the sodium hydroxide pool in cultivating pool cleaning, make pH reach 12.(3) removing the bacterium that at the bottom of the pool, mud capacity of decomposition is strong processes at the bottom of the pool organic, destroys the growing environment of EHP.Although have employed above prevention sterilization method, effect is also not obvious, and liver sausage sporidiosis (EHP) is not controlled at all.
Summary of the invention
Being difficult to prevent the technical problem of radical cure for solving liver sausage sporidiosis in current culture of Penaeus vannamei process, the invention provides a kind of Chinese medicine composition for this disease and and then providing a kind of prevention and controls of this disease.
For realizing above-mentioned technical purpose, the technical solution used in the present invention is: a kind of Chinese medicine composition preventing and treating Penaeus vannamei liver sausage sporidiosis, it is characterized in that, this Chinese medicine composition adopts the material medicine of following weight proportion to combine: Melia azedarach L. 10-20 part, Rhizoma Osmundae 10-20 part, Radix Stemonae 5-15 part, Omphalia 20-30 part, Semen Celosiae 15-25 part, Herba Gelsemii Elegantis 20-30 part, Semen Arecae 15-25 part.
Further, the weight proportion of material medicine is: Melia azedarach L. 15 parts, Rhizoma Osmundae 15 parts, the Radix Stemonae 10 parts, Omphalia 25 parts, Semen Celosiae 20 parts, Herba Gelsemii Elegantis 25 parts, Semen Arecae 20 parts.
The instructions of taking of above-mentioned Chinese medicine composition is: mix in bait after mixing of being pulverized by above-mentioned raw materials medicine and throw something and feed, medicine accounts for 1 ‰-10 ‰ of bait weight.
A kind of prevention and controls utilizing above-mentioned Chinese medicine composition to carry out Penaeus vannamei liver sausage sporidiosis, the method comprises Prevention and Curation two aspect, described prevention refers to and when not falling ill, above-mentioned Chinese medicine composition medicamental pulverata is incorporated at Penaeus vannamei the bait throwing in feeding carrying out prawn in bait, dose accounts for the 1-3 ‰ of bait weight, each continuous use is thrown something and fed 7 days, monthly throws something and feeds for twice; Described treatment refers to that Penaeus vannamei is after infecting liver sausage sporidiosis, above-mentioned Chinese medicine composition medicamental pulverata is incorporated into the bait throwing in feeding carrying out prawn in bait, and dose accounts for the 5-10 ‰ of bait weight, and throw something and feed 15-20 days continuously, once a day.
Experiment proves, this Chinese medicine composition has no side effect to Penaeus vannamei growth, and use rear prawn fast growth, output is high, remarkable in economical benefits, is that a kind of cost is low, prevention effect obvious, by the oral Chinese herbal medicine of carrier.
Detailed description of the invention
Below in conjunction with embodiment, Chinese medicine composition provided by the present invention is described further.
Embodiment 1
Prevent and treat a Chinese medicine composition for Penaeus vannamei liver sausage sporidiosis, this Chinese medicine composition adopts the material medicine of following weight proportion to combine: Melia azedarach L. 15g, Rhizoma Osmundae 15g, Radix Stemonae 10g, Omphalia 25g, Semen Celosiae 20g, Herba Gelsemii Elegantis 25g, Semen Arecae 20g.
The screening formula of said medicine is through the test of many times of applicant, screening, summary and completing, and specific experiment process is as follows:
1, the selection of raw material Chinese herbal medicine and processing technique
(1) selection of this Chinese medicine composition Raw Chinese medicine selects one or more can kill the medicine of pathogen composition with combination according to tcm theory, crude drug selects really on the impact of cultivation object fanout free region, and has hemopoietic or promote the Chinese herbal medicine of body fluid circulatory effect and hepatic cholagogic, Fuzheng Paidu, health invigorating, raising cultivation object autoimmune function.When for this disease of liver sausage sporidiosis, applicant determines compatibility 5 groups of (A, B, C, D, E) pharmaceutical formulations:
A group: Herba Artemisiae Annuae end 10g, Omphalia 25g, Fructus Quisqualis 20g, hundred father-in-law 15g, Rhizoma Osmundae 15g, Radix Et Rhizoma Rhei 10g, Fructus Bruceae 10g, Semen Arecae 20g.
B group: Omphalia 15g, Radix Aucklandiae 15g, Melia azedarach L. 15g, Radix Stemonae 10g, Semen Arecae 2g.
C group: Rhizoma Curcumae 15g, Rhizoma Picrorhizae 20g, crane wind 10g, Semen Arecae 20g, Resina Ferulae 15g, Fructus Quisqualis 20g.
D group: Melia azedarach L. 15 parts, Rhizoma Osmundae 15 parts, the Radix Stemonae 10 parts, Omphalia 25 parts, Semen Celosiae 20 parts, Herba Gelsemii Elegantis 25 parts, Semen Arecae 20 parts.
E group: Herba Gelsemii Elegantis 25g, Borneolum Syntheticum 15g, Semen Celosiae 20g, Melia azedarach L. 15g, Herba Artemisiae Annuae end 10g.
(2) after above-mentioned 5 groups of drug regimens are determined, clayed into power by each material medicine, mixing, by component dress, label is stand-by.
2, the determination of test site and test tank
Heng Bao Aquaculture Corporation of Rongcheng City is cooperation unit.Have selected 6 areas is respectively 25m
2leg Shrimp Litopenaeus vannamei Culture Raceway-type Ponds, point row is that 5 (1#, 2#, 3#, 4#, 5#) medication test tanks and 1 contrast pond (6#) are as Experimental Comparison pond, wherein 5 medication test tanks use by the Chinese medicine composition correspondence of 5 kinds of different compatibilities respectively and carry out medicine drug sensitive test, 6# contrasts a culturing pool in the Stochastic choice factory of pond, requires that this culturing pool is thrown to support from Penaeus vannamei under the prerequisite not using medicine and occurs without liver sausage sporozoon (EHP) and other diseases to prawn results stage breeding pond.
Medication test tank pond number is numbered corresponding use with medicine group kind and is referred to following table.
| Medicine group kind | A | B | C | D | E | Without drug use |
| Chi Hao | 1# | 2# | 3# | 4# | 5# | 6# |
3, the determination of test period
Penaeus vannamei medicine contrast test slightly starts to about 2.5cm point of pond at shrimp Seedling mark, every cube of water body breeding density 400 tail, starts contrast test to end of day in August 10 then on May 18th, 2014.
4, application method and result
4.1 medicines are to the prophylactic tria of prawn disease
On May 21st, 2014 is the day of dispenser first, 5 each formulation rates in medication pond are 1 ‰ of bait weight, connect and feed 7 days, stop dispenser 3 weeks, and then continuously dispenser 7 days, monthly dispenser twice, other daily management is identical with 6# pond production management, to harvesting on August 10, wherein 6# culturing pool occurs without EHP and other condition of illness.Through contrast, 5 medication test tanks to gather in the crops the quality of shrimp and weight and 6# culturing pool basically identical, average prawn survival rate is 75%, average size is 90/kg, and every cube of water body goes out shrimp 3kg, occurs without EHP and other condition of illness, it is even that institute goes out commercial sized prawn size, and build is sturdy.Absolutely prove 5 groups of Chinese medicine compositions carry out mixing bait throw something and feed after to Penaeus vannamei fanout free region, have no side effect.
4.2 medicines are to the therapeutic test of prawn disease
When on June 10th, 2014, shrimp Seedling grew to body long 6-7cm, No. 2 mill portion do not use the pond of Chinese herbal and crude drugs preparations to start to occur poor growth phenomenon, shrimp Seedling is eaten bait and is not but grown, and after finding this kind of state, detects discovery infected liver sausage sporozoon (EHP) through PCR.The infection sporozoan several pond of liver sausage focuses in 5 dispenser ponds through washing and starts medication therapeutic test by once, and administrated method mixes bait by 5 ‰ of the abundance of food to throw something and feed.Prawn in the culturing pool of D group medicine group kind is adopted to come to life after 3 days through observing medication, after 20 days D group medicine group kind culturing pool in prawn sampling well-grown, PCR checks that liver and gall sporozoon disappears substantially, to forming end, best by the prawn growing way of D group medicine group of formula treatment, output is the highest, and output reaches 6.3kg/m
3, comparatively other group output exceeds 20%, and do not occur that liver sausage sporozoon infects again to results, the Chinese medicine composition this being filtered out to D group medicine group of formula is practical in Prevention and Curation liver sausage sporozoon.
Embodiment 2
A kind of Chinese medicine composition preventing and treating Penaeus vannamei liver sausage sporidiosis, it is characterized in that, this Chinese medicine composition adopts the material medicine of following weight proportion to combine: Melia azedarach L. 10g, Rhizoma Osmundae 20g, Radix Stemonae 5g, Omphalia 30g, Semen Celosiae 15g, Herba Gelsemii Elegantis 30g, Semen Arecae 15g.
Prevention and Curation pharmaceutical quantities increases test.
When the Chinese medicine composition proportioning of getting the present embodiment is prevented, pharmaceutical quantities increases test, experimental technique with in embodiment 1 4.1 the method for medicine to the prophylactic tria of prawn disease consistent, 5 each formulation rates in medication pond increase to 2 ‰ of bait weight, connect and feed 7 days, stop dispenser 3 weeks, and then continuous dispenser 7 days, monthly dispenser twice, to the stage of gathering in the crops, occur without EHP and other condition of illness in 5 dispenser ponds.Illustrate thus, the growth that formulation rate is increased to 2 ‰ pairs of prawns has no adverse effects, and prevents liver sausage sporidiosis effect pretty good simultaneously.
When the prawn sick body of liver sausage sporidiosis has been infected in treatment, formulation rate is increased to 7.5 ‰ of bait weight, throw something and feed continuously 15 days, PCR checks that liver and gall sporozoon disappears completely, prawn grows fine, and to the results stage occurring without liver sausage sporidiosis, can prove thus, during treatment, formulation rate can be increased to 7.5 ‰ of bait weight.
Embodiment 3
A kind of Chinese medicine composition preventing and treating Penaeus vannamei liver sausage sporidiosis, it is characterized in that, this Chinese medicine composition adopts the material medicine of following weight proportion to combine: Melia azedarach L. 20g, Rhizoma Osmundae 10g, Radix Stemonae 15g, Omphalia 20g, Semen Celosiae 25g, Herba Gelsemii Elegantis 20g, Semen Arecae 25g.
Prevention and Curation pharmaceutical quantities increases test.
When the Chinese medicine composition proportioning of getting the present embodiment is prevented, pharmaceutical quantities increases test, experimental technique with in embodiment 1 4.1 the method for medicine to the prophylactic tria of prawn disease consistent, 5 each formulation rates in medication pond increase to 3 ‰ of bait weight, connect and feed 7 days, stop dispenser 3 weeks, and then continuous dispenser 7 days, monthly dispenser twice, to the stage of gathering in the crops, occur without EHP and other condition of illness in 5 dispenser ponds.Illustrate thus, the growth that formulation rate is increased to 3 ‰ pairs of prawns has no adverse effects, and prevents liver sausage sporidiosis effect pretty good simultaneously.
When the prawn sick body of liver sausage sporidiosis has been infected in treatment, formulation rate is increased to 10 ‰ of bait weight, throw something and feed continuously 20 days, PCR checks that liver and gall sporozoon disappears completely, prawn grows fine, and to the results stage occurring without liver sausage sporidiosis, can prove thus, during treatment, formulation rate can be increased to 10 ‰ of bait weight.
Claims (3)
1. prevent and treat the Chinese medicine composition of Penaeus vannamei liver sausage sporidiosis for one kind, it is characterized in that, this Chinese medicine composition adopts the material medicine of following weight proportion to combine: Melia azedarach L. 10-20 part, Rhizoma Osmundae 10-20 part, Radix Stemonae 5-15 part, Omphalia 20-30 part, Semen Celosiae 15-25 part, Herba Gelsemii Elegantis 20-30 part, Semen Arecae 15-25 part.
2. prevent and treat the Chinese medicine composition of Penaeus vannamei liver sausage sporidiosis for one kind, it is characterized in that, this Chinese medicine composition adopts the material medicine of following weight proportion to combine: Melia azedarach L. 15 parts, Rhizoma Osmundae 15 parts, the Radix Stemonae 10 parts, Omphalia 25 parts, Semen Celosiae 20 parts, Herba Gelsemii Elegantis 25 parts, Semen Arecae 20 parts.
3. the prevention and controls utilizing the Chinese medicine composition of the control Penaeus vannamei liver sausage sporidiosis described in claim 1 or 2 to carry out Penaeus vannamei liver sausage sporidiosis, the method comprises Prevention and Curation two aspect, described prevention refers to and when not falling ill, above-mentioned Chinese medicine composition medicamental pulverata is incorporated at Penaeus vannamei the bait throwing in feeding carrying out prawn in bait, dose accounts for the 1-3 ‰ of bait weight, each continuous use is thrown something and fed 7 days, monthly throws something and feeds for twice; Described treatment refers to that Penaeus vannamei is after infecting liver sausage sporidiosis, above-mentioned Chinese medicine composition medicamental pulverata is incorporated into the bait throwing in feeding carrying out prawn in bait, and dose accounts for the 5-10 ‰ of bait weight, and throw something and feed 15-20 days continuously, once a day.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106266381A (en) * | 2016-08-12 | 2017-01-04 | 中国水产科学研究院东海水产研究所 | A kind of compound preparation for killing prawn shrimp liver sausage born of the same parents worm and using method thereof |
| CN106994172A (en) * | 2017-06-12 | 2017-08-01 | 海（厦门）生物科技有限公司 | A kind of Chinese medicine composition of prophylactic treatment gut of shrimp microsporidian, pulvis and preparation method thereof |
| CN107582919A (en) * | 2017-10-23 | 2018-01-16 | 天津瑞贝特科技发展有限公司 | Treat Chinese medicine composition of shrimp enteron aisle sporidiosis and preparation method thereof |
| CN109568464A (en) * | 2019-01-17 | 2019-04-05 | 中国水产科学研究院黄海水产研究所 | It is a kind of prevent and treat shrimp crab sporidiosis composition and its application |
| CN112790128A (en) * | 2020-12-31 | 2021-05-14 | 亚太海洋生物科技(厦门)有限公司 | Method for preventing Vertically spreading of liver and intestinal sporozoon of Litopenaeus vannamei |
| CN112831549A (en) * | 2021-02-23 | 2021-05-25 | 广州力必拓生物科技有限公司 | Method for preventing and controlling liver and intestinal sporozoon diseases of penaeus vannamei boone |
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2016
- 2016-02-18 CN CN201610088935.9A patent/CN105561099A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106266381A (en) * | 2016-08-12 | 2017-01-04 | 中国水产科学研究院东海水产研究所 | A kind of compound preparation for killing prawn shrimp liver sausage born of the same parents worm and using method thereof |
| CN106994172A (en) * | 2017-06-12 | 2017-08-01 | 海（厦门）生物科技有限公司 | A kind of Chinese medicine composition of prophylactic treatment gut of shrimp microsporidian, pulvis and preparation method thereof |
| CN106994172B (en) * | 2017-06-12 | 2020-09-01 | 海一（厦门）生物科技有限公司 | Traditional Chinese medicine composition and powder for preventing and treating prawn intestinal microsporidian and preparation method thereof |
| CN107582919A (en) * | 2017-10-23 | 2018-01-16 | 天津瑞贝特科技发展有限公司 | Treat Chinese medicine composition of shrimp enteron aisle sporidiosis and preparation method thereof |
| CN109568464A (en) * | 2019-01-17 | 2019-04-05 | 中国水产科学研究院黄海水产研究所 | It is a kind of prevent and treat shrimp crab sporidiosis composition and its application |
| CN109568464B (en) * | 2019-01-17 | 2023-12-19 | 中国水产科学研究院黄海水产研究所 | Composition for preventing and treating shrimp and crab sporozoosis and application thereof |
| CN112790128A (en) * | 2020-12-31 | 2021-05-14 | 亚太海洋生物科技(厦门)有限公司 | Method for preventing Vertically spreading of liver and intestinal sporozoon of Litopenaeus vannamei |
| CN112790128B (en) * | 2020-12-31 | 2022-06-10 | 亚太海洋生物科技(厦门)有限公司 | Method for preventing Vertically spreading of liver and intestinal sporozoon of Litopenaeus vannamei |
| CN112831549A (en) * | 2021-02-23 | 2021-05-25 | 广州力必拓生物科技有限公司 | Method for preventing and controlling liver and intestinal sporozoon diseases of penaeus vannamei boone |
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| WD01 | Invention patent application deemed withdrawn after publication |