CN102689990B - Method for controlling water quality in shrimp pool by using bacillus and bagasse - Google Patents
Method for controlling water quality in shrimp pool by using bacillus and bagasse Download PDFInfo
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- CN102689990B CN102689990B CN 201210173614 CN201210173614A CN102689990B CN 102689990 B CN102689990 B CN 102689990B CN 201210173614 CN201210173614 CN 201210173614 CN 201210173614 A CN201210173614 A CN 201210173614A CN 102689990 B CN102689990 B CN 102689990B
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- bagasse
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Abstract
A method for controlling water quality in a shrimp pool by using bacillus and bagasse is characterized by comprising the following steps: firstly, preparing bagasse, and loading the bagasse in bacillus culture solution after sterilization for fermentation; throwing the bagasse and the fermented solution uniformly into the shrimp pool; and after throwing the bagasse into the pool, periodically stirring the bagasse and adding more bacillus. The method provided by the invention can reduce water change of the shrimp pool during the whole culturing period, thereby reducing pollution caused by culturing, preventing the nutrients in the pool from draining and pathogens from entering into the pool, lowering the requirements on water usage, and saving the cost. Besides, after the adoption of the method, shrimps have better disease-resistant capability and grow fast, so that culturing output is larger and economical benefits are better.
Description
Technical field
The invention belongs to the sea farming field, particularly relate to a kind of method of utilizing genus bacillus (Bacillus sp.) and bagasse to control water quality in the cement shrimp-cultivation pool, it is thrown in the bagasse of crossing through fermentation of bacillus in aquaculture water, is used for controlling the water quality of cement shrimp-cultivation pool.
Background technology
In the prawn culturing process, the middle and later periods that particularly cultivates, a large amount of accumulation along with organism in the pond, cultivation water very easily worsens, and becomes to induce an illness, and causes the major cause that cultivation is failed.At present control water quality with two kinds of methods: 1. change water treatment, 2. use quality purifying device for water.But change in a large number the pollution that water can cause surrounding enviroment, also can cause simultaneously beneficiating ingredient loss in pond water, as algae, also can greatly increase in addition the possibility that harmful cause of disease enters the pond.The quality purifying device for water cost is higher, it is promoted the use of on a large scale be restricted.Lack at present a kind of beneficiating ingredient that can keep in the pond and do not lose, can reduce again cause of disease and enter the pond, the method for can also improve pond water quality, saving cost.
Summary of the invention
The object of the present invention is to provide a kind of method of utilizing genus bacillus (Bacillus sp.) and bagasse to control water quality in the cement shrimp-cultivation pool, it can satisfy the demand of prior art.
A kind of method of utilizing genus bacillus and bagasse to control water quality in the cement shrimp-cultivation pool is characterized in that first removing the bagasse that rots and go mouldy, and the sieving of recycling screen cloth goes out the bagasse of appropriate length; This bagasse sterilization is placed on is equipped with in genus bacillus (Bacillus sp.) nutrient solution that had fermented, continue fermentation; And then the bagasse that will ferment and fermented liquid evenly drop in water body in the cement shrimp-cultivation pool; After bagasse is put into cement shrimp-cultivation pool water body, to stir at set intervals bagasse and mend and throw genus bacillus.
The present invention can reduce the quantity of exchanged water of the whole breeding process of cement shrimp-cultivation pool, has both reduced breeding wastewater to the pollution of environment, can keep again in the pond beneficiating ingredient not lose and reduce cause of disease and enter the pond, can also alleviate the pressure of breeding water, saves cost.In addition, utilize the prawn that present method is formed to have good resistance against diseases and the speed of growth, make the output of cultivation higher, obtain better economic benefit.
Embodiment
When implementing that the present invention is a kind of utilizes genus bacillus (Bacillus sp.) to control the method for water quality in the cement shrimp-cultivation pool with bagasse, at first remove the bagasse that rots and go mouldy, the sieving of recycling screen cloth goes out the bagasse of appropriate length; This bagasse sterilization is placed on is equipped with in the genus bacillus nutrient solution that had fermented, continue fermentation; And then the bagasse that will ferment and fermented liquid evenly drop in water body in the cement shrimp-cultivation pool, after bagasse is put into the water body of cement shrimp-cultivation pool, will stir at set intervals bagasse and mend throwing genus bacillus (Bacillus sp.).The process of present method operation has namely been completed in above operation.
The specific embodiment of the present invention is as follows
1. bagasse chooses
With 10 eye mesh screen sieving bagasse, the bagasse length that obtains after sieving is 0.5-3cm, selects standby without the bagasse of mildew.Wherein short grained bagasse can be suspended in water body, is conducive to bacterial adhesion, utilization in water body.There is the bagasse of mildew to be attached with miscellaneous bacteria, might to the toxic effect of water body environment, forbids to use.
2. the cleaning of bagasse, sterilization
With high density sterilizing agent (as dioxide peroxide, every cube of water body adds 100g), bagasse was carried out soaking disinfection 12-24 hour, can remove the unwanted bacteria on bagasse.Bagasse after sterilization must clean up with clean sea water, in order to avoid affect ferment effect.
3. the fermentation of bagasse
Get the water in culturing pool, by volume percentage ratio 5%-10% injects genus bacillus (Bacillus sp.) bacterium liquid, aerated culture 18-24 hour.Add above-mentioned bagasse through Screening Treatment again in container, continue fermentation 24-48 hour.The concentration of the bacterium liquid genus bacillus (Bacillus sp.) of injecting reaches 10
9More than cfu/ml.
4. the input of bagasse
The bagasse that fermented is in the shrimp seedling of being rivals in a contest was together poured the culturing pool of having sterilized into together with fermented liquid in front 7 days.After 7 days, put again seedling after at the bottom of bagasse all sinks to the pond.The injected volume that calculates bagasse by the pond floorage is 0.8-1.2kg/m
2, should accomplish even distribution during input, avoid poly-heap phenomenon.Bagasse contains a large amount of Mierocrystalline celluloses, can provide a large amount of carbon sources for microbial growth, can for microbial growth provides attaching surface, be beneficial to microorganism growth again on the other hand.In addition, the loose bagasse in bottom also provides place hidden and that perch for cultured prawn.
5. daily administration
After bagasse is put into cement shrimp-cultivation pool water body, to stir 1 time every 3-5 days.Cultivation in earlier stage should be mended every 10-15 days and throw genus bacillus (Bacillus sp.) 1 time, made the density of genus bacillus in water body (Bacillus sp.) maintain 10
4More than cfu/ml.In the cultivation later stage, because nutritive substance in cement shrimp-cultivation pool water body is abundant, genus bacillus (Bacillus sp.) can amount reproduction, throws genus bacillus (Bacillus sp.) so can not mend.
Bagasse and genus bacillus (Bacillus sp.) are put into the shrimp seedling of being rivals in a contest after cement shrimp-cultivation pool water body, should select health, energetic prawn seedling to put seedling, press 120-150 tail/m
2Density throw in.
In the bed mud of above-mentioned genus bacillus (Bacillus sp.) from Zhoushan prawn culturing pond, Zhejiang Province, screening obtains through purifying.Above-mentioned genus bacillus (Bacillus sp.) bacterium liquid is to cultivate the Bacteria liquid of 24h in the LB nutrient solution under 30 ℃, dark condition, described LB nutrient solution formula is comprised of 1000ml distilled water, 30gNaCl, 10g peptone and 5g yeast extract, and pH is 7.4.Above-mentioned genus bacillus (Bacillus sp.) forms circular bacterium colony on the LB solid medium, smooth surface, and neat in edge, surface wettability, white, opaque, gramstaining is positive.Research finds that the genus bacillus that the present invention uses has extraordinary effect.Above-mentioned genus bacillus (Bacillus sp.) name of screening of selecting is called Shan-1, be deposited on May 7th, 2012 the China Committee for Culture Collection of Microorganisms's common micro-organisms center that is positioned at No. 3, the Chaoyang District Beijing North Star No. 1, West Road institute, deposit number is CGMCC NO.6084.
Claims (1)
1. a method of utilizing genus bacillus and bagasse to control water quality in the cement shrimp-cultivation pool, is characterized in that first removing the bagasse that rots and go mouldy, and the sieving of recycling screen cloth goes out the bagasse of appropriate length; With the sterilization of this bagasse be placed on be equipped with the genus bacillus of having fermented (
BacillusSp.) in nutrient solution, continue fermentation; And then the bagasse that will ferment and fermented liquid evenly drop in water body in the cement shrimp-cultivation pool; After bagasse is put into cement shrimp-cultivation pool water body, to stir at set intervals bagasse and mend to throw genus bacillus (
BacillusSp.); Described genus bacillus (
BacillusSp.) name is called Shan-1, and deposit number is CGMCC NO.6084.
2.
Control as claimed in claim 1 the method for water quality in the cement shrimp-cultivation pool, the length that it is characterized in that described bagasse is 0.5-3cm, and the injected volume of bagasse is calculated as 0.8-1.2kg/m by the pond floorage
2
3. control as claimed in claim 1 the method for water quality in the cement shrimp-cultivation pool, it is characterized in that described stirring bagasse and mend to throw genus bacillus (
BacillusSp.) be to stir 1 time every 3-5 days, cultivation mended every 10-15 days early stage throw genus bacillus (
BacillusSp.) 1 time.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN 201210173614 CN102689990B (en) | 2012-05-30 | 2012-05-30 | Method for controlling water quality in shrimp pool by using bacillus and bagasse |
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| Application Number | Priority Date | Filing Date | Title |
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| CN 201210173614 CN102689990B (en) | 2012-05-30 | 2012-05-30 | Method for controlling water quality in shrimp pool by using bacillus and bagasse |
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| Publication Number | Publication Date |
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| CN102689990A CN102689990A (en) | 2012-09-26 |
| CN102689990B true CN102689990B (en) | 2013-05-08 |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103911361B (en) * | 2014-04-21 | 2016-05-18 | 中国海洋大学 | Biomembrane cultural method and application thereof taking bagasse as carrier |
| CN103896410B (en) * | 2014-04-21 | 2016-05-25 | 中国海洋大学 | A kind of based on biomembranous culturing wastewater processing system |
| CN104585123B (en) * | 2015-01-07 | 2017-01-25 | 中国海洋大学 | Method for controlling quality of industrial aquatic water of prawns and suspending floccules generating device |
| CN112662582B (en) * | 2020-12-28 | 2022-10-11 | 海南大学 | Marine bacterium HNB 15 for producing cellulase, microbial preparation thereof and degradation method of natural cellulose |
| CN112725226B (en) * | 2020-12-28 | 2022-10-11 | 海南大学 | Screening of cellulase-producing bacteria C18 and natural cellulose degradation method of microbial preparation thereof |
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| CN1384187A (en) * | 2001-04-27 | 2002-12-11 | 华东师范大学 | Batch culture process of variant strain of Bacillus subtilis |
| CN1850660A (en) * | 2006-05-17 | 2006-10-25 | 天津生机集团有限公司 | Improved composition of base of aquatic cultivation pool |
| JP2006314858A (en) * | 2005-05-10 | 2006-11-24 | Petroleum Energy Center | Method of purifying soil or water contaminated with heavy oil |
| CN101643280A (en) * | 2009-09-02 | 2010-02-10 | 张培举 | Microbial water-purifying agent and preparation method thereof |
| CN102219310A (en) * | 2011-04-11 | 2011-10-19 | 武汉中傲天乐生物科技有限公司 | Environment-friendly composite microbial preparation, preparation thereof, and application thereof to aquaculture |
| CN102304486A (en) * | 2011-08-31 | 2012-01-04 | 华东师范大学 | Composite bacillus spp. microecological preparation and preparation method thereof |
-
2012
- 2012-05-30 CN CN 201210173614 patent/CN102689990B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1384187A (en) * | 2001-04-27 | 2002-12-11 | 华东师范大学 | Batch culture process of variant strain of Bacillus subtilis |
| JP2006314858A (en) * | 2005-05-10 | 2006-11-24 | Petroleum Energy Center | Method of purifying soil or water contaminated with heavy oil |
| CN1850660A (en) * | 2006-05-17 | 2006-10-25 | 天津生机集团有限公司 | Improved composition of base of aquatic cultivation pool |
| CN101643280A (en) * | 2009-09-02 | 2010-02-10 | 张培举 | Microbial water-purifying agent and preparation method thereof |
| CN102219310A (en) * | 2011-04-11 | 2011-10-19 | 武汉中傲天乐生物科技有限公司 | Environment-friendly composite microbial preparation, preparation thereof, and application thereof to aquaculture |
| CN102304486A (en) * | 2011-08-31 | 2012-01-04 | 华东师范大学 | Composite bacillus spp. microecological preparation and preparation method thereof |
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