CN102689990A - Method for controlling water quality in shrimp pool by using bacillus and bagasse - Google Patents
Method for controlling water quality in shrimp pool by using bacillus and bagasse Download PDFInfo
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- CN102689990A CN102689990A CN2012101736140A CN201210173614A CN102689990A CN 102689990 A CN102689990 A CN 102689990A CN 2012101736140 A CN2012101736140 A CN 2012101736140A CN 201210173614 A CN201210173614 A CN 201210173614A CN 102689990 A CN102689990 A CN 102689990A
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Abstract
A method for controlling water quality in a shrimp pool by using bacillus and bagasse is characterized by comprising the following steps: firstly, preparing bagasse, and loading the bagasse in bacillus culture solution after sterilization for fermentation; throwing the bagasse and the fermented solution uniformly into the shrimp pool; and after throwing the bagasse into the pool, periodically stirring the bagasse and adding more bacillus. The method provided by the invention can reduce water change of the shrimp pool during the whole culturing period, thereby reducing pollution caused by culturing, preventing the nutrients in the pool from draining and pathogens from entering into the pool, lowering the requirements on water usage, and saving the cost. Besides, after the adoption of the method, shrimps have better disease-resistant capability and grow fast, so that culturing output is larger and economical benefits are better.
Description
Technical field
The invention belongs to the sea farming field; Particularly relate to a kind of method of utilizing genus bacillus (Bacillus sp.) and bagasse to control water quality in the cement shrimp-cultivation pool; It is thrown in the bagasse of crossing through fermentation of bacillus in the aquaculture water, is used for controlling the water quality of cement shrimp-cultivation pool.
Background technology
In the prawn culturing process, the middle and later periods of particularly culturing, along with a large amount of accumulation of organism in the pond, cultivation water very easily worsens, and becomes to induce an illness, and causes the major cause of culturing failure.At present control water quality: 1. change water treatment, 2. use quality purifying device for water with two kinds of methods.But change the pollution that water can cause surrounding enviroment in a large number, also can cause in the pond water beneficiating ingredient to be lost simultaneously,, also can increase the possibility that harmful cause of disease gets into the pond in addition greatly like algae.The quality purifying device for water cost is higher, it is promoted the use of on a large scale be restricted.Lack at present a kind of beneficiating ingredient that can keep in the pond and do not lose, can reduce cause of disease again and get into the pond, the method for can also improve pond water quality, practicing thrift cost.
Summary of the invention
The object of the present invention is to provide a kind of method of utilizing genus bacillus (Bacillus sp.) and bagasse to control water quality in the cement shrimp-cultivation pool, it can satisfy the demand of prior art.
A kind of method of utilizing water quality in genus bacillus and the bagasse control cement shrimp-cultivation pool is characterized in that removing the bagasse that rots and go mouldy earlier, utilizes mesh screen to leach the bagasse of appropriate length again; The sterilization of this bagasse is placed on is equipped with in genus bacillus (Bacillus sp.) nutrient solution that had fermented, continue fermentation; And then the bagasse that will ferment and fermented liquid evenly drop in the water body in the cement shrimp-cultivation pool; After bagasse is put into cement shrimp-cultivation pool water body, to stir bagasse at set intervals and mend the throwing genus bacillus.
The present invention can reduce the quantity of exchanged water of the whole breeding process of cement shrimp-cultivation pool, has both reduced the pollution of breeding wastewater to environment, can keep in the pond beneficiating ingredient not lose again and reduce cause of disease getting into the pond, can also alleviate the pressure of breeding water, practices thrift cost.In addition, the prawn that utilizes present method to form has the good resistance against diseases and the speed of growth, makes the output of breed higher, obtains better economic benefit.
Embodiment
Embodiment of the present invention is a kind of when utilizing the method for water quality in genus bacillus (Bacillus sp.) and the bagasse control cement shrimp-cultivation pool, at first removes the bagasse that rots and go mouldy, and utilizes mesh screen to leach the bagasse of appropriate length again; The sterilization of this bagasse is placed on is equipped with in the genus bacillus nutrient solution that had fermented, continue fermentation; And then the bagasse that will ferment and fermented liquid evenly drop in the water body in the cement shrimp-cultivation pool, after bagasse is put into the water body of cement shrimp-cultivation pool, will stir bagasse at set intervals and mend throwing genus bacillus (Bacillus sp.).More than the process of present method operation has promptly been accomplished in operation.
Embodiment of the present invention is following
1. bagasse chooses
With 10 eye mesh screen sieving bagasse, the bagasse length that obtains after the sieving is 0.5-3cm, selects the bagasse of no mildew subsequent use.Wherein short grained bagasse can be suspended in the water body, helps bacterial adhesion, utilization in the water body.There is the bagasse of mildew to be attached with assorted bacterium, might the toxic effect of water body environment be forbidden to use.
2. the cleaning of bagasse, sterilization
With high density sterilizing agent (like dioxide peroxide, every cube of water body adds 100g) bagasse was carried out soaking disinfection 12-24 hour, can remove the unwanted bacteria on the bagasse.Bagasse after the sterilization must clean up with clean sea water, in order to avoid influence ferment effect.
3. the fermentation of bagasse
Get the water of culturing in the pond, by volume percentage ratio 5%-10% injects genus bacillus (Bacillus sp.) bacterium liquid, aerobic culture 18-24 hour.In container, add above-mentioned bagasse again, continue fermentation 24-48 hour through Screening Treatment.The concentration of the bacterium liquid genus bacillus (Bacillus sp.) of injecting reaches 10
9More than the cfu/ml.
4. the input of bagasse
The bagasse that fermented is together poured in the already sterilised culturing pool together with fermented liquid preceding 7 days of the shrimp seedling of being rivals in a contest.After 7 days, treat to put seedling again after bagasse all sinks at the bottom of the pond.The injected volume that calculates bagasse by the pond floorage is 0.8-1.2kg/m
2, should accomplish uniform distribution during input, avoid gathering the heap phenomenon.Bagasse contains a large amount of Mierocrystalline celluloses, can a large amount of carbon sources be provided for microbial growth, can be beneficial to microorganism growth for microbial growth provides attaching surface again on the other hand.In addition, the loose bagasse in bottom also provides place hidden and that perch for cultured prawn.
5. daily administration
After bagasse is put into cement shrimp-cultivation pool water body, whenever to stir 1 time at a distance from 3-5 days.Culture early stage, every should the benefit at a distance from 10-15 days thrown genus bacillus (Bacillus sp.) 1 time, makes the density of genus bacillus in the water body (Bacillus sp.) maintain 10
4More than the cfu/ml.Culture the later stage, because nutritive substance is abundant in the cement shrimp-cultivation pool water body, genus bacillus (Bacillus sp.) can breed in a large number, throws genus bacillus (Bacillus sp.) so can not mend.
Bagasse and genus bacillus (Bacillus sp.) are put into the shrimp seedling of being rivals in a contest behind the cement shrimp-cultivation pool water body, should select for use health, energetic prawn seedling to put seedling, press 120-150 tail/m
2Density throw in.
In the bed mud of above-mentioned genus bacillus (Bacillus sp.) from prawn culturing pond, Zhoushan, Zhejiang Province city, screening obtains through purifying.Above-mentioned genus bacillus (Bacillus sp.) bacterium liquid is the bacterium bacterium liquid of in the LB nutrient solution, cultivating 24h under 30 ℃, dark condition; Said LB nutrient solution prescription is made up of 1000ml zero(ppm) water, 30gNaCl, 10g peptone and 5g yeast extract, and pH is 7.4.Above-mentioned genus bacillus (Bacillus sp.) forms circular bacterium colony on the LB solid medium, smooth surface, and neat in edge, surface wettability, white, opaque, gramstaining is positive.Discover that the genus bacillus that the present invention uses has extraordinary effect.Above-mentioned genus bacillus (Bacillus sp.) name of screening of selecting for use is called Shan-1; Be deposited in the China Committee for Culture Collection of Microorganisms common micro-organisms center that is positioned at No. 3, No. 1 institute in Chaoyang District Beijing North Star West Road on May 7th, 2012, deposit number is CGMCC NO.6084.
Claims (4)
1. a method of utilizing water quality in genus bacillus and the bagasse control cement shrimp-cultivation pool is characterized in that removing the bagasse that rots and go mouldy earlier, utilizes mesh screen to leach the bagasse of appropriate length again; The sterilization of this bagasse is placed on is equipped with in genus bacillus (Bacillus sp.) nutrient solution that had fermented, continue fermentation; And then the bagasse that will ferment and fermented liquid evenly drop in the water body in the cement shrimp-cultivation pool; After bagasse is put into cement shrimp-cultivation pool water body, to stir bagasse at set intervals and mend throwing genus bacillus (Bacillus sp.).
2. control the method for water quality in the cement shrimp-cultivation pool according to claim 1, the length that it is characterized in that said bagasse is 0.5-3cm, and the injected volume of bagasse is calculated as 0.8-1.2kg/m by the pond floorage
2
3. control the method for water quality in the cement shrimp-cultivation pool according to claim 1, it is characterized in that described genus bacillus (Bacillus sp.) name is called Shan-1, deposit number is CGMCC NO.6084.
4. control the method for water quality in the cement shrimp-cultivation pool according to claim 1; It is characterized in that described stirring bagasse and mend throwing genus bacillus (Bacillus sp.) for whenever stirring 1 time at a distance from 3-5 days, early stage was whenever mended throwing genus bacillus (Bacillus sp.) 1 time at a distance from 10-15 days in breed.
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| Application Number | Priority Date | Filing Date | Title |
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| CN 201210173614 CN102689990B (en) | 2012-05-30 | 2012-05-30 | Method for controlling water quality in shrimp pool by using bacillus and bagasse |
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| Application Number | Priority Date | Filing Date | Title |
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| CN 201210173614 CN102689990B (en) | 2012-05-30 | 2012-05-30 | Method for controlling water quality in shrimp pool by using bacillus and bagasse |
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| CN102689990A true CN102689990A (en) | 2012-09-26 |
| CN102689990B CN102689990B (en) | 2013-05-08 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103896410A (en) * | 2014-04-21 | 2014-07-02 | 中国海洋大学 | Biofilm-based mariculture wastewater treatment system |
| CN103911361A (en) * | 2014-04-21 | 2014-07-09 | 中国海洋大学 | Biofilm cultivation method taking bagasse as carrier and application thereof |
| CN104585123A (en) * | 2015-01-07 | 2015-05-06 | 中国海洋大学 | Suspending floccules generating device used for controlling quality of industrial aquatic water of prawns |
| CN112662582A (en) * | 2020-12-28 | 2021-04-16 | 海南大学 | Marine bacterium HNB 15 for producing cellulase, microbial preparation thereof and degradation method of natural cellulose |
| CN112725226A (en) * | 2020-12-28 | 2021-04-30 | 海南大学 | Screening of cellulase-producing bacterium C18 and natural cellulose degradation method of microbial preparation thereof |
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| CN102219310A (en) * | 2011-04-11 | 2011-10-19 | 武汉中傲天乐生物科技有限公司 | Environment-friendly composite microbial preparation, preparation thereof, and application thereof to aquaculture |
| CN102304486A (en) * | 2011-08-31 | 2012-01-04 | 华东师范大学 | Composite bacillus spp. microecological preparation and preparation method thereof |
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Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1384187A (en) * | 2001-04-27 | 2002-12-11 | 华东师范大学 | Batch culture process of variant strain of Bacillus subtilis |
| JP2006314858A (en) * | 2005-05-10 | 2006-11-24 | Petroleum Energy Center | Method of purifying soil or water contaminated with heavy oil |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103896410A (en) * | 2014-04-21 | 2014-07-02 | 中国海洋大学 | Biofilm-based mariculture wastewater treatment system |
| CN103911361A (en) * | 2014-04-21 | 2014-07-09 | 中国海洋大学 | Biofilm cultivation method taking bagasse as carrier and application thereof |
| CN103911361B (en) * | 2014-04-21 | 2016-05-18 | 中国海洋大学 | Biomembrane cultural method and application thereof taking bagasse as carrier |
| CN103896410B (en) * | 2014-04-21 | 2016-05-25 | 中国海洋大学 | A kind of based on biomembranous culturing wastewater processing system |
| CN104585123A (en) * | 2015-01-07 | 2015-05-06 | 中国海洋大学 | Suspending floccules generating device used for controlling quality of industrial aquatic water of prawns |
| CN112662582A (en) * | 2020-12-28 | 2021-04-16 | 海南大学 | Marine bacterium HNB 15 for producing cellulase, microbial preparation thereof and degradation method of natural cellulose |
| CN112725226A (en) * | 2020-12-28 | 2021-04-30 | 海南大学 | Screening of cellulase-producing bacterium C18 and natural cellulose degradation method of microbial preparation thereof |
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| CN102689990B (en) | 2013-05-08 |
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