CN103896410A - Biofilm-based mariculture wastewater treatment system - Google Patents
Biofilm-based mariculture wastewater treatment system Download PDFInfo
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- CN103896410A CN103896410A CN201410161735.2A CN201410161735A CN103896410A CN 103896410 A CN103896410 A CN 103896410A CN 201410161735 A CN201410161735 A CN 201410161735A CN 103896410 A CN103896410 A CN 103896410A
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- bacillus
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Abstract
The invention discloses a biofilm-based mariculture wastewater treatment system which comprises multiple buckets, the multiple buckets are connected in series with each other, and a bracket is arranged in the bucket; the first bucket is provided with a water spraying device, water in the tail-end bucket flows back to a culture pond again; a biofilm is fixed on the bracket, and a culture method thereof comprises the steps of filling bagasse sieved by using a sieve in a mesh bag, and putting the mesh bag on the bracket; pumping culture pond water into a container until an attached base is submerged, adding activated bacillus (Bacillus sp.) powder and adding nutritive salts; adding activated bacillus powder and nutritive salts again 10-15 days later, wherein the name of the used bacillus powder is Shan-1; and maturing by culturing for 25-30 days. The system disclosed by the invention is simple and feasible, low in cost and long in period of use, and has an extremely strong practical application value in mariculture wastewater treatment. The biofilm adopted by the invention can survive for a long time on bagasse, and has a strong processing capacity to mariculture wastewater, especially has strong degradation capability to ammonia nitrogen and nitrate nitrogen in mariculture wastewater.
Description
Technical field
The invention belongs to marine culture wastewater process field, be specifically related to a kind of based on biomembranous culturing wastewater processing system, to process the waste water producing in sea farming.
Background technology
In sea farming, the environmental problem of discharge of wastewater more and more causes people's attention, and in breeding wastewater is processed, the superiority of biomembrance process wastewater processing technology, is just being subject to people and is more and more paying close attention to.People as carrier, inoculate different strain culturing microbial films with different materials, utilize the Degradation of microorganism to process breeding wastewater.Conventional carrier has at present: sand, rubble, gravel, plastic honeycomb, haydite, elastic filler, coke, slag, asbestos tile etc.In contrast to this, with bagasse as carrier have specific surface area large, relatively cheap, easily the characteristic such as obtain.In addition, along with the slow resolution of bagasse provides the nutritive substances such as carbon source for bacterium, combine with the bacterium with efficient performance, can reach desirable result of use.
Summary of the invention
The object of the present invention is to provide a kind of based on biomembranous culturing wastewater processing system, can utilize bagasse to inoculate genus bacillus (Bacillus sp.) as carrier and cultivate microbial film and process the method for breeding wastewater, it can meet marine culture wastewater and process that needed design cost is low, energy consumption is low, purification efficiency is high, do not produce the demands such as secondary pollution.
A kind of based on biomembranous culturing wastewater processing system, it is characterized in that comprising 3-5 water tank of mutual series connection, the multilayer bracket made from corrosion resistant material is housed in described water tank, the water outlet of the water inlet pipe of each water tank be arranged on bottom and the water outlet of water tank top make each Water in Water Tanks body all to lower and on mobile; First water tank arranges a liquid distribution with water pump, and the water in last water tank flows back to culturing pool again; On described support, be fixed with and cultivate in accordance with the following methods ripe microbial film;
Described biomembranous cultural method comprises: the preparation process of adherance and biomembranous culturing step,
Described adherance preparation process: filter out the bagasse that diameter is 250 μ m-600 μ m with screen cloth, sterilization packs the mesh bag of being made by 60 mesh sieve thin,tough silk into after cleaning, and each mesh bag bagasse thickness is 1.5-2.5cm, then mesh bag is placed on the support in container;
Described microbial film culturing step: to the seawater immersion adherance that pumps into culturing pool in said vesse, regulate aeration quantity to make dissolved oxygen in container water body maintain 5-6mg/L by gas stone; According to water body volume, add genus bacillus (Bacillus sp.) bacterium powder after activated make container endospore bacillus quantity reach 106-107cfu/mL and add NH4Cl and molasses as nutritive salt; After 10-15 days, add one time of nutrition salt and with concentration genus bacillus (Bacillus sp.); Described genus bacillus bacterium powder strain name used is BZ-5;
Be 22 ‰-35 ‰ at sea water salinity, under temperature 28-33 ℃, PH7.5-8.5 condition, altogether experience 25-30 days, or in the time adhering to bacterium on every gram of bagasse and be not less than 1010cfu, described mesh bag becomes cultivates ripe microbial film.
Each support 3-5 layer, every layer of one deck aperture of all tiling is the grid screen cloth of 15mm-30mm; The microbial film of described cultivation maturation is arranged on this screen cloth,
Each support volume is the 40%-50% of water tank volume.
The water tank cumulative volume for the treatment of system is breeding wastewater day quantity discharged 1-2 times.
Described nutrient concentration is respectively by NH4Cl20mg/L, molasses 0.3g/L.
Described genus bacillus bacterium powder is to make through cryogenic freezing after fermentation, and after activation, cell concentration is about 1010-1011cfu/g.
Above-mentioned genus bacillus (Bacillus sp.) name of selecting screening is called BZ-5, be deposited on March 14th, 2014 the China Committee for Culture Collection of Microorganisms's common micro-organisms center that is positioned at No. 3, No. 1, Chaoyang District Beijing North Star West Road institute, deposit number is CGMCC NO.8919.
Simple possible of the present invention, cost is low, the usage period is long, in marine culture wastewater is processed, has very strong actual application value.Microbial film of the present invention can be survived for a long time on bagasse, and breeding wastewater is had to very strong processing power, particularly the ammonia nitrogen in breeding wastewater and nitrite nitrogen is had to very strong degradation capability.
Accompanying drawing explanation
Fig. 1 is overall structure schematic diagram of the present invention.
Fig. 2 is the structural representation of support of the present invention.
Wherein, 1, water tank, 2, liquid distribution, 3, support, 4, water pipe, 5, grid screen cloth, 6, mesh bag, 7, water tank.
Embodiment
The microbial film that native system adopts is cultivated maturation in accordance with the following methods take bagasse as carrier: the preparation process and the biomembranous culturing step that comprise bagasse adherance:
Described bagasse adherance preparation process: filter out the bagasse that diameter is 250 μ m-600 μ m with screen cloth, sterilization packs the mesh bag of being made by 60 mesh sieve thin,tough silk into after cleaning, in each mesh bag, bagasse thickness is 1.5-2.5cm, then mesh bag is placed on the support in container;
Described microbial film culturing step: to the seawater immersion adherance that pumps into culturing pool in said vesse, regulate aeration quantity to make dissolved oxygen in system maintain 5-6mg/L by gas stone; According to water body volume, genus bacillus (Bacillus sp.) the bacterium powder adding after activated makes container endospore bacillus quantity reach 10
6-10
7cfu/mL also adds NH
4cl and molasses are as nutritive salt; Described nutrient concentration is respectively NH
4cl20mg/L, molasses 0.3g/L, add one time of nutrition salt and with concentration genus bacillus (Bacillus sp.) after 10-15 days; Described genus bacillus bacterium powder strain name used is BZ-5;
Be 22 ‰-35 ‰ at sea water salinity, under temperature 28-33 ℃, PH7.5-8.5 condition, cultivate and after 25-30 days, on every gram of bagasse, adhere to bacterium and can reach 10
10cfu, has adhered to ripe microbial film on described bagasse adherance.Described genus bacillus bacterium powder is to make through cryogenic freezing after fermentation, and after activation, cell concentration is 10
10-10
11cfu/g.
Structure and the use-pattern of native system are as follows:
1. the foundation of support and treatment system: first treatment system as shown in Figure 1, is made up of several circular thickening water tanks 1, and each water tank 1 is as a processing unit, and cumulative volume is about breeding wastewater day quantity discharged 1-2 doubly; First processing unit is equipped with a liquid distribution 2, and the water that liquid distribution 2 drips is input to the water tank bottom of this processing unit after collecting via water tank 7, and in last processing unit, water can flow back to culturing pool 3 again; Each processing unit is placed a support 3, and water pipe 4 connects, the water outlet of the water inlet pipe of each processing unit be arranged on bottom and water outlet top make in each bucket water body all to lower and on mobile;
Described support 2 as shown in Figure 2, is made as pvc pipe of corrosion resistant mechanically resistant material, each support 3-5 layer, and every layer of grid screen cloth 5 that tiling one deck aperture is 15mm-30mm, each support 2 volumes account for the 40%-50% of processing unit.
2. adherance preparation: filter out the bagasse that length is 250 μ m-600 μ m with screen cloth, sterilization packs the mesh bag 6 of being made by 60 mesh sieve thin,tough silk into after cleaning, and each mesh bag 6 fills bagasse 1.0-1.2Kg, then mesh bag 6 is fixed on support screen cloth 5; Every layer of screen cloth 5 one bag of bagasse that tiles, water body vertical current is crossed bagasse, can increase greatly the contact area of microbial film and water body, increases degradation efficiency; The 60 large small-bore of order mesh bags can wrap up bagasse and have good mistake water-based energy, enter water body along with the slow degradation of bagasse has the tiny bagasse of part, this can not only promote biomembranous update also can increase carbon-nitrogen ratio in water body and promote microbial growth;
3. in the manner described above microbial film is cultivated, cultivated maturation through 25-30 days microbial films, ripe rear bagasse adheres to bacterium and can reach 10
10cfu/g left and right, energy fast degradation breeding wastewater;
4. wastewater treatment: waste water is inputted to first processing unit via liquid distribution, again flow back to culturing pool from last processing unit; In the time that the initial ammonia nitrogen concentration of processing is 5.98mg/L breeding wastewater, after 72h, ammonia nitrogen concentration drops to 0.22mg/L, and clearance, up to 96%, can be adjusted hydraulic detention times to obtain maximum efficiency according to the different pollution levels of breeding wastewater.
In the bed mud of above-mentioned genus bacillus (Bacillus sp.) from prawn culturing pond, Zhoushan, Zhejiang Province, through purifying, screening obtains.Above-mentioned genus bacillus (Bacillus sp.) is formed as circular bacterium colony on LB solid medium, smooth surface, and neat in edge, surface wettability, white, opaque, gramstaining is positive.
Above-mentioned genus bacillus (Bacillus sp.) bacterium powder is made through fermentation cryogenic freezing, cell concentration approximately 10
10-10
11cfu/g.Above-mentioned genus bacillus (Bacillus sp.) name of selecting screening is called BZ-5, be deposited on March 14th, 2014 the China Committee for Culture Collection of Microorganisms's common micro-organisms center that is positioned at No. 3, No. 1, Chaoyang District Beijing North Star West Road institute, deposit number is CGMCC NO.8919.
Claims (6)
1. one kind based on biomembranous culturing wastewater processing system, it is characterized in that comprising 3-5 water tank of mutual series connection, the multilayer bracket made from corrosion resistant material is housed in described water tank, the water outlet of the water inlet pipe of each water tank be arranged on bottom and the water outlet of water tank top make each Water in Water Tanks body all to lower and on mobile; First water tank arranges a liquid distribution with water pump, and the water in last water tank flows back to culturing pool again; On described support, be fixed with and cultivate in accordance with the following methods ripe microbial film;
Described biomembranous cultural method comprises: the preparation process of adherance and biomembranous culturing step,
Described adherance preparation process: filter out the bagasse that diameter is 250 μ m-600 μ m with screen cloth, sterilization packs the mesh bag of being made by 60 mesh sieve thin,tough silk into after cleaning, and each mesh bag bagasse thickness is 1.5-2.5cm, then mesh bag is placed on the support in container;
Described microbial film culturing step: to the seawater immersion adherance that pumps into culturing pool in said vesse, regulate aeration quantity to make dissolved oxygen in system maintain 5-6mg/L by gas stone; According to water body volume, genus bacillus (Bacillus sp.) the bacterium powder adding after activated makes container endospore bacillus quantity reach 10
6-10
7cfu/mL also adds NH
4cl and molasses are as nutritive salt; After 10-15 days, add one time of nutrition salt and with concentration genus bacillus (Bacillus sp.); Described genus bacillus bacterium powder strain name used is BZ-5; Be 22 ‰-35 ‰ at sea water salinity, under temperature 28-33 ℃, PH7.5-8.5 condition, while altogether experiencing 25-30 days, described mesh bag becomes cultivates ripe microbial film.
2. culturing wastewater processing system as claimed in claim 1, is characterized in that each support 3-5 layer, and every layer of one deck aperture of all tiling is the grid screen cloth of 15mm-30mm; The microbial film of described cultivation maturation is arranged on this screen cloth.
3. culturing wastewater processing system as claimed in claim 1, is characterized in that each support volume is the 40%-50% of water tank volume.
4. culturing wastewater processing system as claimed in claim 1, the water tank cumulative volume that it is characterized in that treatment system is breeding wastewater day quantity discharged 1-2 times.
5. culturing wastewater processing system as claimed in claim 1, is characterized in that described nutrient concentration is respectively by NH
4cl20mg/L, molasses 0.3g/L.
6. culturing wastewater processing system as claimed in claim 1, it is characterized in that described genus bacillus bacterium powder is to make through cryogenic freezing after fermentation, and after activation, cell concentration is about 10
10-10
11cfu/g.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106125580A (en) * | 2016-07-25 | 2016-11-16 | 黑龙江省畜牧研究所 | Cultivating wastewater purification device and processing method |
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| CN102674561A (en) * | 2012-05-30 | 2012-09-19 | 中国海洋大学 | Preparation method for immobilized spherules for mariculture waste water treatment |
| CN102689990A (en) * | 2012-05-30 | 2012-09-26 | 中国海洋大学 | Method for controlling water quality in shrimp pool by using bacillus and bagasse |
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2014
- 2014-04-21 CN CN201410161735.2A patent/CN103896410B/en active Active
Patent Citations (6)
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| CN1245151A (en) * | 1999-08-06 | 2000-02-23 | 中国科学院广州地球化学研究所 | Microbe treatment system for improving water quality of aquaculture pond |
| JP2006122771A (en) * | 2004-10-27 | 2006-05-18 | Chuden Kankyo Technos Co Ltd | Fluid treatment method and fluid treatment system |
| CN101343114A (en) * | 2008-08-16 | 2009-01-14 | 王光玉 | Biological denitrification method for wastewater of industrial cultivation |
| CN102225805A (en) * | 2011-04-07 | 2011-10-26 | 江苏省农业科学院 | Biomembrane reactor for improving water quality of water sources, and its preparation method and application |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106125580A (en) * | 2016-07-25 | 2016-11-16 | 黑龙江省畜牧研究所 | Cultivating wastewater purification device and processing method |
| CN106125580B (en) * | 2016-07-25 | 2018-10-12 | 黑龙江省畜牧研究所 | Cultivating wastewater purification device |
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| CN103896410B (en) | 2016-05-25 |
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Inventor after: Dan Hongwei Inventor after: Wang Xianfeng Inventor after: Ma Shen Inventor after: Wei Yifeng Inventor after: Ye Jianyong Inventor before: Ma Shen Inventor before: Wang Xianfeng Inventor before: Dan Hongwei Inventor before: Wei Yifeng Inventor before: Ye Jianyong |
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