CN103923868B - Mixed bacterial microbial preparation and the application in disposing of sewage thereof - Google Patents
Mixed bacterial microbial preparation and the application in disposing of sewage thereof Download PDFInfo
- Publication number
- CN103923868B CN103923868B CN201410181469.XA CN201410181469A CN103923868B CN 103923868 B CN103923868 B CN 103923868B CN 201410181469 A CN201410181469 A CN 201410181469A CN 103923868 B CN103923868 B CN 103923868B
- Authority
- CN
- China
- Prior art keywords
- water
- sewage
- mixed bacterial
- microbial preparation
- pseudomonas
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of mixed bacterial microbial preparation, the bacteria suspension be made up of series bacillus, Pseudomonas stutzeri, pseudomonas pseudoalcaligenes and Pseudomonas oleovorans, total viable count is 3.8*10
8more than CFU/ml.Described mixed bacterial microbial preparation may be used for disposing of sewage, artificial wetland treatment polluted river water and the ability of town domestic sewage in autumn can be strengthened, during embody rule, method is: be added in artificial wet land system in autumn by mixed bacterial microbial preparation of the present invention, and add-on is 1% of sewage volume; Treatment time is more than 7 days or 7 days.After testing, after adopting mixed bacterial microbial preparation of the present invention to dispose of sewage 7 days, the content of the polluted river water water outlet COD after process, total nitrogen, total phosphorus reaches " water environment quality standard " III class water quality standard, and the content's index of town domestic sewage water outlet COD, ammonia nitrogen and total phosphorus after process reaches " urban wastewater treatment firm pollutant emission standard " one-level B standard.
Description
Technical field
The present invention relates to a kind of mixed bacterial microbial preparation, and the application in disposing of sewage, especially strengthen autumn artificial wetland treatment polluted river water and town domestic sewage ability in application, belong to environmental technology field.
Background technology
Artificial swamp is a kind of by artificial construction and supervision and control, and the ground similar with swampland, it utilizes the physics in natural ecosystems, chemistry and biological triple synergies to realize dirty water purification.According to foreign data, the construction costs of general artificial swamp and working cost are only 1/10 ~ 1/2 of traditional second order activity sludge, and unit sewage power consumption and sewage disposal expense are starkly lower than traditional wastewater treatment plant.In artificial wet land system, the activity of microorganism is the principal element of wastewater through organic matter degradation, is primary biological colony and the undertaker of absorption and degradation of contaminant.What play a major role in the removal of nitrogen is Ammonifying bacteria, nitrite bacteria and denitrifying bacterium.The removal of phosphorus absorbing and the absorption of Wetland Substrate mainly through plant and part microorganism.
Summer Plants of Constructed Wetlands riotous growth, in water body, microbial activities is vigorous, can organism in better utilised sewage, and nitrogen phosphoric, has good cleaning action to sewage.But during autumn, wetland plant is ripe, and growth metabolism weakens, and weakens the sorption of nitrogen phosphoric.Simultaneously along with the reduction of temperature, in wet land system, the metabolism of microorganism weakens, therefore in the fall by increasing the quantity of plant rhizosphere superior microorganism, adopting microbial technique strengthened artificial wet land to strengthen Pollution abatement dynamics, is the optimal path improving removal efficiency of nitrogen and phosphorus, save pollution treatment cost.The quantity of direct increase rhizospheric microorganism has become the development trend improving wetland variation effect.
Summary of the invention
For above-mentioned prior art, in view of the wide application prospect of microbial augmentation artificial wetland process in sewage disposal, the invention provides a kind of mixed bacterial microbial preparation, and strengthen autumn artificial wetland treatment polluted river water and town domestic sewage ability in application.
The present invention is achieved by the following technical solutions:
A kind of mixed bacterial microbial preparation, the bacteria suspension be made up of series bacillus, Pseudomonas stutzeri, pseudomonas pseudoalcaligenes and Pseudomonas oleovorans, total viable count of four kinds of bacterium is 3.8 × 10
8more than CFU/ml.
Further, described series bacillus is selected from the bacterial classification that deposit number is CCTCCNO:M2011120, this bacterium classification called after: Paenibacillussp.XP1, preservation date is on April 10th, 2011, depositary institution is: China typical culture collection center, preservation address is: China. Wuhan. and Wuhan University, postcode 430072.Be documented in another Chinese patent of applicant, the patent No. 201110121394.2, publication number 102220265A.
Further, described Pseudomonas stutzeri is selected from the bacterial classification that deposit number is CCTCCNO:M2011431, this bacterium classification called after PseudomonasstutzeriXP2, preservation date is on November 27th, 2011, depositary institution is: China typical culture collection center, preservation address is: China. Wuhan. and Wuhan University, postcode 430072.Be documented in another Chinese patent application of applicant, number of patent application 201210201671.5, publication number 102899263A.
Further, described pseudomonas pseudoalcaligenes is selected from the bacterial classification that deposit number is CCTCCNO:M2012225, this bacterium classification called after PseudomonaspseudoalcaligenesCL-1, preservation date is on June 13rd, 2012, depositary institution is: China typical culture collection center, preservation address is: China. Wuhan. and Wuhan University, postcode 430072.Be documented in another Chinese patent application of applicant, number of patent application 201310032730.5, publication number 103114062A.
Further, described Pseudomonas oleovorans is selected from the bacterial classification that deposit number is CCTCCNO:M2012226, this bacterium classification called after PseudomonasoleovoransCL-3, preservation date is on June 13rd, 2012, depositary institution is: China typical culture collection center, preservation address is: China. Wuhan. and Wuhan University, postcode 430072.Be documented in another Chinese patent of applicant, the patent No. 201210269891.1, publication number 102776145A.
The preparation method of described mixed bacterial microbial preparation is as follows:
By the series bacillus transfering loop scraping 1 ring lawn that inclined-plane is preserved, be seeded in 200mL denitrification nutrient solution, 30 ~ 35 DEG C, 180 ~ 220r/min, shaking table concussion cultivation 24 ~ 36h, obtains the strain cultured solution of activation; Strain cultured solution is centrifugal 15 ~ 20min under 5000 ~ 8000r/min, goes supernatant liquor to obtain somatic cells, is diluted to the bacteria suspension of 1.5g wet thallus/L, obtains series bacillus bacteria suspension with sterilized water; Same method (step, parameter, substratum are all identical) prepares Pseudomonas stutzeri bacteria suspension, pseudomonas pseudoalcaligenes bacteria suspension and Pseudomonas oleovorans bacteria suspension; By four kinds of bacteria suspension equal volume amounts mixing, mixing, obtains mixed bacterial microbial preparation.
Consisting of of described denitrification nutrient solution: in every 1L denitrification nutrient solution, containing following component: CH
3cOONa, 2g; KH
2pO
4, 0.4g; MgSO
47H
2o, 0.6g; CaCl
22H
2o, 0.07g; KNO
3, 1g; Tris damping fluid 12mL; Trace element 2mL; Surplus is water; PH value is 7.0 ~ 7.2.During preparation, by each component mixing mixing, adjust ph to 7.0 ~ 7.2 (sodium hydroxide solution or hydrochloric acid regulate), 110 DEG C of autoclaving 20min, to obtain final product.
Preferably, described trace element is composed of the following components: EDTA50.0g, ZnSO
42.2g, CaCl
25.5g, MnCl
24H
2o5.06g, FeSO
47H
2o5.0g, (NH
4)
6mo
7o
24H
2o1.1g, CuSO
47H
2o1.57g, CoCl
26H
2o1.61g, distilled water 1000ml, during preparation, by each component mixing mixing, to obtain final product.
Described mixed bacterial microbial preparation may be used for disposing of sewage, artificial wetland treatment polluted river water and the ability of town domestic sewage in autumn can be strengthened, by filler, interaction between plant and microorganism, through processes such as absorption, ion-exchange, plant absorption and microbiological deteriorations, the removal efficiency of pollutent in polluted river water and town domestic sewage effectively can be improved.During embody rule, method is: be added to by mixed bacterial microbial preparation of the present invention in artificial wet land system in autumn (such as autumn Reed Constructed Wetland system), add-on is 1% of sewage volume; Treatment time (hydraulic detention time of sewage in drowned flow artificial wet land) is more than 7 days or 7 days.After testing, after adopting mixed bacterial microbial preparation of the present invention to dispose of sewage 7 days, the content of the polluted river water water outlet COD after process, total nitrogen, total phosphorus reaches " water environment quality standard " (GB3838-2002) III class water quality standard, and the content's index of town domestic sewage water outlet COD, ammonia nitrogen and total phosphorus after process reaches " urban wastewater treatment firm pollutant emission standard " (GB18918-2002) one-level B standard.
Described autumn, the structure of artificial swamp was: comprise matrix, the high 600 ~ 650mm of matrix, diameter 450 ~ 500mm, water storage is about 20L.Is provided with rising pipe apart from 50mm place, matrix bottom in matrix, rising pipe is provided with valve for controlling and water outlet.In matrix, water level control pipe is installed, for the water level being subject to processing sewage controlled in wetland.Be provided with water inlet pipe above matrix, water inlet pipe is provided with water-in, for injecting tap water or be subject to processing sewage in wetland.Be provided with metalling, fine sand layer and pedosphere successively from down to up in matrix, wherein, metalling is filled by the rubble of particle diameter 10 ~ 20mm and is formed, and thickness is 250 ~ 300mm; Fine sand layer is filled by the fine sand of particle diameter 1 ~ 2mm and is formed, and thickness is 50-100mm; Pedosphere is filled by soil and is formed, and thickness is 100-150mm.Pedosphere is planted and is implanted with the abundant reed of rhizospheric microorganism.Reed seedling is implanted in artificial wet land system from March kind, watered with gram nutritive medium (in prior art existing conventional products) every 2 days, after one month, start to water tap water (flowing into wet land system by water-in), within every 2 days, change water once, when changing water, open valve, discharged by water outlet.When disposing of sewage, change and be subject to processing sewage and water.
Described matrix is the right cylinder of PVC material.
The present inventor is through research and practice repeatedly, find: will by series bacillus (Paenibacillussp.), Pseudomonas stutzeri (Pseudomonasstutzeri), the mixed bacterial microbial preparation that pseudomonas pseudoalcaligenes (Pseudomonaspseudoalcaligenes) and Pseudomonas oleovorans (Pseudomonasoleovorans) form is added in artificial wet land system, the quantity of rhizosphere denitrogenation flora can be increased, the effect of microorganism in strengthening wetland in autumn, can effectively improve the removal efficiency being subject to processing dirty water pollutant, polluted river water water outlet COD after process, total nitrogen, the content of total phosphorus can reach " water environment quality standard " (GB3838-2002) III class water quality standard, town domestic sewage water outlet COD after process, the content's index of ammonia nitrogen and total phosphorus can reach " urban wastewater treatment firm pollutant emission standard " (GB18918-2002) one-level B standard.
Accompanying drawing explanation
Fig. 1: the present invention is in order to process the small-scale artificial wetland setting drawing of polluted river water and town domestic sewage.
Wherein, 1, water-in; 2, sewage is subject to processing; 3, pedosphere; 4, fine sand layer; 5, gravel bed; 6, reed; 7, observation of stage pipe; 8, valve; 9, water outlet.
Fig. 2: the COD design sketch in mixed bacterial microbial preparation strengthening Reed Constructed Wetland process in autumn polluted river water.
Fig. 3: the total nitrogen design sketch in mixed bacterial microbial preparation strengthening Reed Constructed Wetland process in autumn polluted river water.
Fig. 4: the total phosphorus design sketch in mixed bacterial microbial preparation strengthening Reed Constructed Wetland process in autumn polluted river water.
Fig. 5: the COD design sketch of mixed bacterial microbial preparation strengthening Reed Constructed Wetland process in autumn town domestic sewage.
Fig. 6: the ammonia nitrogen design sketch in mixed bacterial microbial preparation strengthening Reed Constructed Wetland process in autumn town domestic sewage.
Fig. 7: the total phosphorus design sketch in mixed bacterial microbial preparation strengthening Reed Constructed Wetland process in autumn town domestic sewage.
Embodiment
Below in conjunction with embodiment, the present invention is further illustrated.Embodiment for illustration of the present invention, instead of limits the present invention.
Embodiment 1 prepares mixed bacterial microbial preparation
Preparation method is as follows:
By the series bacillus transfering loop scraping 1 ring lawn that inclined-plane is preserved, be seeded in 200mL denitrification nutrient solution, 30 ~ 35 DEG C, 180r/min, 24h is cultivated in shaking table concussion, obtains the strain cultured solution of activation; Strain cultured solution is centrifugal 20min under 8000r/min, goes supernatant liquor to obtain somatic cells, is diluted to the bacteria suspension of 1.5g wet thallus/L, obtains series bacillus bacteria suspension with sterilized water; Same method prepares Pseudomonas stutzeri bacteria suspension, pseudomonas pseudoalcaligenes bacteria suspension and Pseudomonas oleovorans bacteria suspension; By four kinds of bacteria suspension equal volume amounts mixing, mixing, obtain mixed bacterial microbial preparation, after testing, in mixed bacterial microbial preparation, class bacillus viable count is 3.6 × 10
8cFU/ml, Pseudomonas stutzeri viable count is 4.2 × 10
8cFU/ml, pseudomonas pseudoalcaligenes viable count is 4.0 × 10
8cFU/ml, Pseudomonas oleovorans viable count is 3.4 × 10
10cFU/ml.
Described series bacillus, deposit number is CCTCCNO:M2011120, and Classification And Nomenclature is: Paenibacillussp.XP1, preservation date is on April 10th, 2011, depositary institution is: China typical culture collection center, and preservation address is: China. Wuhan. and Wuhan University, postcode 430072.
Described Pseudomonas stutzeri, deposit number is CCTCCNO:M2011431, Classification And Nomenclature is PseudomonasstutzeriXP2, preservation date is on November 27th, 2011, depositary institution is: China typical culture collection center, preservation address is: China. Wuhan. and Wuhan University, postcode 430072.
Described pseudomonas pseudoalcaligenes, deposit number is CCTCCNO:M2012225, Classification And Nomenclature is PseudomonaspseudoalcaligenesCL-1, preservation date is on June 13rd, 2012, depositary institution is: China typical culture collection center, preservation address is: China. Wuhan. and Wuhan University, postcode 430072.
Described Pseudomonas oleovorans, deposit number is CCTCCNO:M2012226, Classification And Nomenclature is PseudomonasoleovoransCL-3, preservation date is on June 13rd, 2012, depositary institution is: China typical culture collection center, preservation address is: China. Wuhan. and Wuhan University, postcode 430072.
Consisting of of described denitrification nutrient solution: in every 1L denitrification nutrient solution, containing following component: CH
3cOONa, 2g; KH
2pO
4, 0.4g; MgSO
47H
2o, 0.6g; CaCl
22H
2o, 0.07g; KNO
3, 1g; Tris damping fluid 12mL; Trace element 2mL; Surplus is water; PH value is 7.0.During preparation, by each component mixing mixing, adjust ph to 7.0 (sodium hydroxide solution or hydrochloric acid regulate), 110 DEG C of autoclaving 20min, to obtain final product.
Described trace element is composed of the following components: EDTA50.0g, ZnSO
42.2g, CaCl
25.5g, MnCl
24H
2o5.06g, FeSO
47H
2o5.0g, (NH
4)
6mo
7o
24H
2o1.1g, CuSO
47H
2o1.57g, CoCl
26H
2o1.61g, distilled water 1000ml, during preparation, by each component mixing mixing, to obtain final product.
The application of embodiment 2 mixed bacterial microbial preparation in the ability strengthening artificial wetland treatment polluted river water in autumn
As shown in Figure 1, the structure of small-scale artificial wetland is: comprise matrix (for PVC material right cylinder), high 600 ~ 650mm, diameter 450 ~ 500mm, water storage is about 20L.Is provided with rising pipe apart from 50mm place, matrix bottom in matrix, rising pipe is provided with valve 8 for controlling and water outlet 9.Water level control pipe 7 is installed, for the water level being subject to processing sewage 2 controlled in wetland in matrix.Be provided with water inlet pipe above matrix, water inlet pipe is provided with water-in 1, for injecting tap water or be subject to processing sewage in wetland.Be provided with metalling 5, fine sand layer 4 and pedosphere 3 from down to up successively in matrix, wherein, metalling 5 is filled by the rubble of particle diameter 10 ~ 20mm and is formed, and thickness is 250 ~ 300mm; Fine sand layer 4 is filled by the fine sand of particle diameter 1 ~ 2mm and is formed, and thickness is 50-100mm; Pedosphere 3 is filled by soil and is formed, and thickness is 100-150mm.Pedosphere 3 is planted and is implanted with the abundant reed 6 of rhizospheric microorganism.Reed seedling is implanted in artificial wet land system from March kind, watered with gram nutritive medium (in prior art existing conventional products) every 2 days, after one month, start to water tap water (flowing into wet land system by water-in 1), within every 2 days, change water once, when changing water, open valve 8, discharged by water outlet 9.The experiment starting period previous moon, change and be subject to processing sewage and water, within every 2 days, change water once, wait for that experiment starts.
Experiment: autumn (9 ~ November), carries out the experiment of mixed bacterial microbial augmentation process polluted river water, when ground temperature is at 12 ~ 20 DEG C to above-mentioned small-sized reed wetland.After experiment starts, mixed bacterial microbial preparation embodiment 1 prepared adds in artificial swamp in the ratio of 1% (percent by volume), in operational process, average every day from small-sized wetland bottom water outlet pipe part take water sample, sewage stops 7 days in small-scale artificial wetland.Meanwhile, not add mixed bacterial microbial preparation for control group.
Concrete treatment effect is shown in Fig. 2, Fig. 3, Fig. 4.Mixed bacterial strengthening reed wetland is compared with control group, to the removal effect obvious (see Fig. 2) of COD, when adding bacterium experimental group the 7th day, CODcr is 16.2mg/L (≤20mg/L), clearance reaches 75.7%, III class water quality standard (CODcr≤20mg/L) that water quality reaches " water environment quality standard " (GB3838-2002).Control group CODcr is still below standard the 7th day time.Fig. 3 shows, and in experimentation, reed adds TN concentration in bacterium strengthening wetland and presents significantly reduced trend, has more excellent removal effect compared with control group.When the 7th day, TN concentration is 0.6mg/L (≤1.0mg/L), and clearance reaches 96.65%, reaches " water environment quality standard " (GB3838-2002) III class water quality standard (TN≤1.0mg/L).And the clearance of control group is only 38.5%.Adding of mixed bacterial, significant effect is served to the denitrogenation of reed wetland strengthening in autumn town domestic sewage.Fig. 4 shows, and add bacterium group compared with control group, TP concentration significantly reduces.When the 7th day, the TN concentration adding bacterium group is 0.15mg/L (<0.2mg/L), clearance reaches 90.4%, reach " water environment quality standard " (GB3838-2002) III class water quality standard (TP≤0.2mg/L), adding of mixed bacterial enhances the removal of reed wetland to TP in polluted river water.
The application of embodiment 3 mixed bacterial microbial preparation in the ability strengthening artificial wetland treatment town domestic sewage in autumn
Autumn (9 ~ November) carries out the experiment of mixed bacterial microbial augmentation process town domestic sewage, when ground temperature is at 12 ~ 20 DEG C to small-sized reed wetland (in embodiment 2).Experiment start after, by the mixed bacterial microbial preparation of activation in 1% ratio add in artificial swamp, in operational process, average every day from each small-sized wetland bottom water outlet pipe part take water sample, sewage stops 7 days in small-scale artificial wetland.Meanwhile, not add mixed bacterial microbial preparation for control group.
Concrete treatment effect is shown in Fig. 5, Fig. 6, Fig. 7.Mixed bacterial strengthening reed wetland is compared with control group, to the removal effect obvious (see Fig. 5) of COD, when adding bacterium experimental group the 4th day, CODcr is 55mg/L (≤60mg/L), clearance reaches 67.6%, one-level B standard (CODcr≤60mg/L) that water quality reaches " urban wastewater treatment firm pollutant emission standard " (GB18918-2002).When the 7th day, adding bacterium group clearance is 85.3%, control group clearance 63.5%.Control group CODcr is up to standard the 7th day time.Fig. 6 shows, and in experimentation, reed adds NH in bacterium strengthening wetland
3-N concentration presents the trend reduced gradually, has more excellent removal effect compared with control group.NH when the 7th day
3-N concentration is down to 6.2mg/L (≤8mg/L), and clearance reaches 87.4%, water quality reaches " urban wastewater treatment firm pollutant emission standard " (GB18918-2002) one-level B standard (NH
3-N≤8mg/L).And control group clearance is 52.64%, below standard.Fig. 7 shows, add bacterium strengthening experimental group compared with control group, TP concentration significantly reduces, when the 7th day, TN concentration is 0.4mg/L (<0.5mg/L), clearance reaches 88.9%, and one-level B standard that water quality reaches " urban wastewater treatment firm pollutant emission standard " (GB18918-2002) requires (TP≤0.5mg/L).And the TP clearance of control group is 65%, below standard.
Integrated embodiment 2,3, shows, the device applied in the present invention is simple to operate, without the need to aeration in operational process, takes full advantage of the good action of microorganism, can reach emission standard, can effectively save process operation cost.Carry out biological reinforced by adding mixed bacterial to artificial swamp in autumn, during hydraulic detention time 7 days, COD, N, P content's index being subject to processing sewage reaches " water environment quality standard " (GB3838-2002) III class water quality standard or " urban wastewater treatment firm pollutant emission standard " (GB18918-2002) one-level B standard.
Claims (7)
1. a mixed bacterial microbial preparation, is characterized in that: the bacteria suspension be made up of series bacillus, Pseudomonas stutzeri, pseudomonas pseudoalcaligenes and Pseudomonas oleovorans, and wherein, viable count is 3.8 × 10
8more than CFU/ml, described Pseudomonas stutzeri is selected from the bacterial classification that deposit number is CCTCCNO:M2011431, this bacterium classification called after PseudomonasstutzeriXP2, preservation date is on November 27th, 2011, depositary institution is: China typical culture collection center, preservation address is: China. Wuhan. and Wuhan University, postcode 430072;
Described series bacillus is selected from the bacterial classification that deposit number is CCTCCNO:M2011120, this bacterium classification called after: Paenibacillussp.XP1, preservation date is on April 10th, 2011, depositary institution is: China typical culture collection center, preservation address is: China. Wuhan. and Wuhan University, postcode 430072;
Described pseudomonas pseudoalcaligenes is selected from the bacterial classification that deposit number is CCTCCNO:M2012225, this bacterium classification called after PseudomonaspseudoalcaligenesCL-1, preservation date is on June 13rd, 2012, depositary institution is: China typical culture collection center, preservation address is: China. Wuhan. and Wuhan University, postcode 430072;
Described Pseudomonas oleovorans is selected from the bacterial classification that deposit number is CCTCCNO:M2012226, this bacterium classification called after PseudomonasoleovoransCL-3, preservation date is on June 13rd, 2012, depositary institution is: China typical culture collection center, preservation address is: China. Wuhan. and Wuhan University, postcode 430072.
2. the preparation method of mixed bacterial microbial preparation according to claim 1, it is characterized in that: the series bacillus transfering loop scraping 1 ring lawn that inclined-plane is preserved, be seeded in 200mL denitrification nutrient solution, 30 ~ 35 DEG C, 180 ~ 220r/min, shaking table concussion cultivation 24 ~ 36h, obtains the strain cultured solution of activation; Strain cultured solution is centrifugal 15 ~ 20min under 5000 ~ 8000r/min, goes supernatant liquor to obtain somatic cells, is diluted to the bacteria suspension of 1.5g wet thallus/L, obtains series bacillus bacteria suspension with sterilized water; Same method prepares Pseudomonas stutzeri bacteria suspension, pseudomonas pseudoalcaligenes bacteria suspension and Pseudomonas oleovorans bacteria suspension; By four kinds of bacteria suspension equal volume amounts mixing, mixing, obtains mixed bacterial microbial preparation;
Consisting of of described denitrification nutrient solution: in every 1L denitrification nutrient solution, containing following component: CH
3cOONa, 2g; KH
2pO
4, 0.4g; MgSO
47H
2o, 0.6g; CaCl
22H
2o, 0.07g; KNO
3, 1g; Tris damping fluid 12mL; Trace element 2mL; Surplus is water; PH value is 7.0 ~ 7.2; During preparation, by each component mixing mixing, adjust ph to 7.0 ~ 7.2,110 DEG C of autoclaving 20min, to obtain final product.
3. the application of mixed bacterial microbial preparation according to claim 1 in disposing of sewage.
4. mixed bacterial microbial preparation according to claim 1 strengthen autumn artificial wetland treatment polluted river water and town domestic sewage ability in application.
5. the application according to claim 3 or 4, is characterized in that: during embody rule, and method is: be added in artificial wet land system in autumn by mixed bacterial microbial preparation, and add-on is 1% of sewage volume; Treatment time is more than 7 days or 7 days.
6. application according to claim 5, is characterized in that: described autumn, the structure of artificial swamp was: comprise matrix, the high 600 ~ 650mm of matrix, diameter 450 ~ 500mm; Is provided with rising pipe apart from 50mm place, matrix bottom in matrix, rising pipe is provided with valve for controlling and water outlet; In matrix, water level control pipe is installed; Be provided with water inlet pipe above matrix, water inlet pipe is provided with water-in; Be provided with metalling, fine sand layer and pedosphere successively from down to up in matrix, wherein, metalling is filled by the rubble of particle diameter 10 ~ 20mm and is formed, and thickness is 250 ~ 300mm; Fine sand layer is filled by the fine sand of particle diameter 1 ~ 2mm and is formed, and thickness is 50-100mm; Pedosphere is filled by soil and is formed, and thickness is 100-150mm; Pedosphere is planted and is implanted with reed.
7. application according to claim 6, is characterized in that: reed seedling is implanted in artificial wet land system from March kind, waters, after one month, starts to water and pour water, within every 2 days, change water once every 2 days with gram nutritive medium; When disposing of sewage, change and be subject to processing sewage and water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410181469.XA CN103923868B (en) | 2014-04-30 | 2014-04-30 | Mixed bacterial microbial preparation and the application in disposing of sewage thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410181469.XA CN103923868B (en) | 2014-04-30 | 2014-04-30 | Mixed bacterial microbial preparation and the application in disposing of sewage thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103923868A CN103923868A (en) | 2014-07-16 |
| CN103923868B true CN103923868B (en) | 2016-04-06 |
Family
ID=51142267
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410181469.XA Active CN103923868B (en) | 2014-04-30 | 2014-04-30 | Mixed bacterial microbial preparation and the application in disposing of sewage thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103923868B (en) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106676021A (en) * | 2015-11-06 | 2017-05-17 | 丹阳市尚德生物科技有限公司 | Microbial preparation for river course water quality restoration, and preparation method thereof |
| CN105347481B (en) * | 2015-11-26 | 2017-11-03 | 山东大学 | The method that biofermentation coupling artificial wet land system purifies low ratio of carbon to ammonium sanitary sewage |
| CN105347514A (en) * | 2015-12-10 | 2016-02-24 | 郭嘉川 | Sewage treatment accelerant and preparation method thereof |
| CN105481102B (en) * | 2015-12-30 | 2018-03-02 | 中林山水（北京）生态科技股份有限公司 | A kind of remodeling method of promoting to a higher rank with microbial cleaning skill upgrading sewage effluents water quality |
| CN107555608B (en) * | 2017-08-29 | 2023-11-17 | 暨南大学 | Method and system for removing microcystis and toxins in water body by strengthening constructed wetland |
| CN109105237A (en) * | 2018-06-26 | 2019-01-01 | 南京工程学院 | Remove and measure the experimental method that wetland plant rhizosphere microorganism group influences phosphorus in plant absorption water |
| CN109576189B (en) * | 2018-12-29 | 2022-04-19 | 合肥市东方美捷分子材料技术有限公司 | Composite microbial inoculum for black and odorous water body and preparation method and application thereof |
| CN110241050B (en) * | 2019-07-04 | 2021-05-28 | 江苏宜裕环保科技有限公司 | Compound autotrophic nitrifying bacteria, preparation method of solid microbial inoculum thereof and application of compound autotrophic nitrifying bacteria in sewage treatment containing high salinity and high ammonia nitrogen |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1566642A (en) * | 2003-06-26 | 2005-01-19 | 乐金电子(天津)电器有限公司 | Frame of hermetic compressor and its manufacturing method |
| CN101941762A (en) * | 2010-09-08 | 2011-01-12 | 福建微水环保技术有限公司 | Method for treating wastewater by utilizing composite microbial preparation |
| WO2012079140A1 (en) * | 2010-12-14 | 2012-06-21 | Gustavo Casal De Rey | Biostimulation and bioenhancing processes for treating domestic and industrial effluents |
| CN102583775A (en) * | 2012-02-14 | 2012-07-18 | 福建微水环保技术有限公司 | Composite microorganism preparation and application thereof in waste water treatment |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101238262B1 (en) * | 2010-09-14 | 2013-03-06 | 김준홍 | The microbial seeds which having excellent wastewater treatment ability and producing method for the sames and the wastewater treatment method |
-
2014
- 2014-04-30 CN CN201410181469.XA patent/CN103923868B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1566642A (en) * | 2003-06-26 | 2005-01-19 | 乐金电子(天津)电器有限公司 | Frame of hermetic compressor and its manufacturing method |
| CN101941762A (en) * | 2010-09-08 | 2011-01-12 | 福建微水环保技术有限公司 | Method for treating wastewater by utilizing composite microbial preparation |
| WO2012079140A1 (en) * | 2010-12-14 | 2012-06-21 | Gustavo Casal De Rey | Biostimulation and bioenhancing processes for treating domestic and industrial effluents |
| CN102583775A (en) * | 2012-02-14 | 2012-07-18 | 福建微水环保技术有限公司 | Composite microorganism preparation and application thereof in waste water treatment |
Non-Patent Citations (2)
| Title |
|---|
| 污水处理中微生物反硝化脱氮过程及代谢规律;吉芳英等;《四川理工学院学报(自然科学版)》;20130831;第26卷(第4期);第10-14页 * |
| 生活污水高效降解混合菌群的研究;王国惠等;《给水排水》;20051231;第31卷(第5期);第44-46页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103923868A (en) | 2014-07-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103923868B (en) | Mixed bacterial microbial preparation and the application in disposing of sewage thereof | |
| CN106754514B (en) | A kind of sustainable dredging removes the microbial bacterial agent preparation method of black and odorous water | |
| CN106186343B (en) | A kind of comprehensive processing method to urban river | |
| Lu et al. | Study on method of domestic wastewater treatment through new-type multi-layer artificial wetland | |
| CN103820427B (en) | The preparation method of microorganism purification of water quality microbial inoculum | |
| CN100378272C (en) | Stereo in-situ ecological urban water system repairing process | |
| CN105110476B (en) | A kind of three-dimensional for the smelly river water quality strengthened purification of urban black is biological floating bed | |
| CN104692536A (en) | Aquatic plant fixing device for repairing water and bottom mud | |
| CN108911158B (en) | A kind of rural domestic sewage treatment system based on small watershed form | |
| CN110240286B (en) | Black and odorous water body treatment method based on biochar immobilized composite microorganism treatment and aeration reoxygenation system thereof | |
| CN101224940A (en) | River sediment in-situ remediation method | |
| CN102276110B (en) | Method for remedying micro-polluted water body and subsurface flow artificial wetland system | |
| CN102757132B (en) | Method for treating rural domestic sewage by utilizing denitrifying phosphate-accumulating organisms and invigorated artificial wetland | |
| CN105174471A (en) | Reinforced black odorous river microecological reconstruction/balance and water quality improvement method | |
| CN102464404B (en) | Method and system for remediation and treatment of polluted river water | |
| CN105585223B (en) | A kind of freshwater aquiculture waste water advanced treatment recovery system and method | |
| CN104232546A (en) | Construction method and application of immobilized biological bacterium agent for micro-polluted water source | |
| CN106007191A (en) | Black odorous river course ecological treatment system and treatment method thereof | |
| CN108070543A (en) | It is a kind of to be suitable for preparations and application of the low C/N than the denitrogenation bacteria preparation of wastewater treatment | |
| CN102776140B (en) | Cold-tolerant pseudomonas strain Den-05, and screening method and application thereof | |
| CN103130377A (en) | Ecological control and bioremediation coupling technology of water area pollution sources | |
| Ni et al. | Demonstration research project of a new three-stage bio-oxidation pond for purifying black smelly water bodies | |
| CN202729889U (en) | Sewage treatment artificial wetland system | |
| CN109095728A (en) | A kind of rainfall runoff purification device and purification method | |
| CN210103566U (en) | Riverway water quality normal position lift system |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |