CN105174471A - Reinforced black odorous river microecological reconstruction/balance and water quality improvement method - Google Patents

Reinforced black odorous river microecological reconstruction/balance and water quality improvement method Download PDF

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CN105174471A
CN105174471A CN201510588894.5A CN201510588894A CN105174471A CN 105174471 A CN105174471 A CN 105174471A CN 201510588894 A CN201510588894 A CN 201510588894A CN 105174471 A CN105174471 A CN 105174471A
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river
bacillus
water
micro organism
organism fungicide
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赵亚勋
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    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A20/00—Water conservation; Efficient water supply; Efficient water use
    • Y02A20/40—Protecting water resources
    • Y02A20/402—River restoration
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W10/00—Technologies for wastewater treatment
    • Y02W10/10—Biological treatment of water, waste water, or sewage

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention relates to a reinforced black odorous river microecological reconstruction/balance and water quality improvement method. When the method is used for treatment, composite microbes are utilized. The composite microbes contain a composite microbial inoculant A, a composite microbial inoculant B and a composite microbial inoculant C in a weight ratio of (6-25):(5-10):(15-35). The composite microbial inoculant A comprises Rhodopseudomonas spheroides; the composite microbial inoculant B comprises lactic-acid-producing Bacillus; and the composite microbial inoculant C comprises low-temperature organic mineralization bacterium. The Rhodopseudomonas spheroides, lactic-acid-producing Bacillus and low-temperature organic mineralization bacterium can adapt to the growth environment of the river course, and have specific treatment capacity for the river course.

Description

A kind of method strengthening the reconstruction of black-odor river Tiny ecosystem and balance and water correction
Technical field
The present invention relates to a kind of method of pollution administration, particularly relate to a kind of method strengthening the reconstruction of black-odor river Tiny ecosystem and balance and water correction.
Background technology
The activity intensive due to socioeconomic develop rapidly, the mankind and the irregular discharge of high density pollution thing, cause urban river water quality and substrate severe contamination, water body self-purification ability is poor, and water power is not enough, and ecological functions are degenerated completely.Urban river water body black foul sewage dye has become the global people's livelihood and problem of environmental pollution, one of most important restraining factors having become China's socio-economic development.Under the overall situation of the enforcement of national major scientific and technological project " water pollution control technology and harnessing project ", various places government is in policy, regulation, engineering, the aspects such as science and technology and education take series of measures, set up black-odor river to harness the river office, set up pollution treatment special fund, the scientific research strength that annual input is a large amount of and pollution treatment fund, but because pollution treatment theory falls behind, governing measure is single, practical experience lacks, cause lacking combining property and continuity between experiment research and development theory and concrete engineering enforcement, make the limitation of pollution treatment technology and fragmentary increasing, the technology of waste water control system of complete set could not be formed, therefore, the key technology of water pollution Control and treatment is resolved far away, this has also become the key subjects of domestic and international environment specialist's research.
The main following methods means that adopt process the pollution of river both at home and abroad at present.
1, cutting dirty method: be generally adopt to control waste water control method, is the object reaching waste water control by reducing the pollutent entering water body.But this method relates to the progressively enforcement of a series of macro policies such as water body river basin planning, and the cycle of producing effects is long, is difficult to take the fragmentary point source in rural area and pollution of area source into account.
2, desilting method: be usual a kind of means controlling bottom and pollute both at home and abroad, but its engineering running amount large, with high costs, subsequent disposal cannot be carried out, easily damage is caused to river levee, riverbed, easily cause secondary pollution;
3, the training of channel: the pollutent in bed mud in river can shift away by dredging engineering in the short period of time, but the ecosystem in destructible river course itself, exogenous organic pollutant cannot be decomposed, provide the condition of sheltering evil people and countenance evil practices, not reach the object of purifying water body;
4, water transfer moisturizing: water transfer, moisturizing make again running water circulate.Can by the mode of diluting and shift, the water quality in river be purified in the short period of time, but the condition required for the method is more special, need to utilize the method for special river level difference or water pump lifting to realize the exchange of water, the method just allows pollutent shift to other water bodys, cannot pollutent be removed, cure the symptoms, not the disease;
5, flocculation agent method: though flocculation, coagulation can eliminate the suspended substance of water body fast, remove black smelly less effective, and time of coagulation is not long, easy bounce-back, cannot organic pollutant be removed, very easily cause secondary pollution, the object of river water and environmental protection can not be realized;
6, stabilization pond method: have that simple structure, capital construction working cost are low, removal efficiency advantages of higher, but there is the problems such as floor space is large, sewage collecting is complicated, and bed mud alluvial is serious;
7, pasture and water and biological floating island is planted: its cleaning principle utilizes plant roots and stems to carry out nutrients uptake, but this method is the supplementary means in pollution treatment process, can not as Main Means, single employing can not fundamentally contaminated solution problem.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of method strengthening the reconstruction of black-odor river Tiny ecosystem and balance and water correction.
The present invention solves the problems of the technologies described above adopted technical scheme: a kind of method strengthening the reconstruction of black-odor river Tiny ecosystem and balance and water correction, comprises the steps:
(1) complex microorganism is prepared, be that 6 ~ 25:5 ~ 10:15 ~ 35 mix by weight by complex micro organism fungicide A, complex micro organism fungicide B and complex micro organism fungicide C, obtained complex microorganism, described complex micro organism fungicide A comprises Rhodopseudomonas spheroides, described complex micro organism fungicide B comprises bacillus JY-LZ, and described complex micro organism fungicide C comprises the organic mineralized bacterium of low temperature;
(2) Biological Pretreatment is carried out to target river course; Gushing planting aquatic plants with main each of bordering on of gushing, and throwing in described complex microorganism 30 ~ 320ppm/ days, described waterplant and complex microorganism form biological reinforced band;
(3) the biological internal recycle respiratory system gushed in river and cometabolism mechanism is built; Gush interior layout molecule bubble oxygen to transmit and surperficial oxygenation combination aeration system in target river, to promote space flourish and the morphology control of complex microorganism;
(4) advanced treatment of polluted bed mud and reduction, balance bed mud structure, sets up river and gushes bottom original position step circular treatment microorganism bed; Gush interior input bio-based, biological brick and high molecule microcapsule to target river, and drop into described complex microorganism 30 ~ 320ppm/ days, make the carbon-nitrogen ratio C/N of adjustment aim river water burst body be 4:1 ~ 8:1, N/P ratio N/P is 7:1 ~ 25:1, and biological carbon is 2.3 ~ 6.2PPM; To carry out bio-oxidation to polluted bed mud, row oxygenation process is poured into target river simultaneously;
(5) ecological management of water body and deep purifying, rebuilds river and gushes microecosystem and balance; Planting aquatic plants builds biological floating island, continues to throw in described complex microorganism 30 ~ 320ppm/ days, and make the carbon-nitrogen ratio C/N of adjustment aim river water burst body be 4:1 ~ 8:1, N/P ratio N/P is 7:1 ~ 25:1;
(6) water ecology reparation and multi-functional regulation and control, put hydrocoles in gushing to target river, and continue to throw in described complex microorganism 30 ~ 320ppm/ days, and make the carbon-nitrogen ratio C/N of adjustment aim river water burst body be 4:1 ~ 8:1, N/P ratio N/P is 7:1 ~ 25:1.
Wherein, described complex micro organism fungicide A comprises following bacterial classification: spherical Rhodopseudomonas: Rhodopseudomonas palustris: capsula Rhodopseudomonas: withered grass bud robe bacillus: bacillus cereus: Bacillus licheniformis: denitrifying bacterium: milk-acid bacteria: yeast: the weight ratio of colloid bacillus cereus is 2.0 ~ 8.0:1.5 ~ 5.8:1.0 ~ 5.0:2.0 ~ 6.0:1.2 ~ 4.6:1.0 ~ 4.0:2.0 ~ 6.5:1.0 ~ 3.5:0.5 ~ 3.0:0.3 ~ 3.2.
Wherein, described complex micro organism fungicide B comprises following bacterial classification: bacillus JY-LZ: spherical Rhodopseudomonas: bacillus coagulans: bacillus laterosporus: Bacillus licheniformis: milk-acid bacteria: yeast: actinomycetes: the weight ratio of azotobacter chroococcum is 1.6 ~ 5.6:0.5 ~ 2.5:1.3 ~ 6.5:1.0 ~ 4.0:1.0 ~ 3.0:0.5 ~ 1.2:0.5 ~ 2.5:0.2 ~ 1.5:0.4 ~ 2.8.
Wherein, described complex micro organism fungicide C comprises following bacterial classification: the organic mineralized bacterium of low temperature: Rhodopseudomonas palustris: withered grass bud robe bacillus: colloid bacillus cereus: bacillus laterosporus: bacillus pumilus: nitrobacteria: denitrifying bacterium: milk-acid bacteria: yeast: the weight ratio of azotobacter chroococcum is 2.0 ~ 10:1.0 ~ 5.0:1.5 ~ 7.5:1.2 ~ 6.4:1.0 ~ 3.5:1.0 ~ 3.0:0.6 ~ 4.0:1.5 ~ 5.8:0.5 ~ 4.5:0.5 ~ 2.5:0.4 ~ 3.5.
Wherein, screen, cultivate described Rhodopseudomonas spheroides, bacillus JY-LZ, sample needed for the organic mineralized bacterium of low temperature respectively from target canal reactor and/or bed mud.
Wherein, in step (2), the fraction of coverage of described waterplant is 1 ~ 5%;
Preferably, described waterplant is one or more in reed, Caulis Miscanthis floriduli, Scirpus tabernaemontani, aquatic Canna generalis Bailey, cyperus alternifolius;
Preferably, the planting density of described waterplant is 15 ~ 30 strains/m 2;
Preferably, in step (4), described bio-based is containing, for example the component of lower massfraction: medical stone 20% ~ 35%, high molecule microcapsule 35% ~ 75%;
Preferably, the particle diameter of described medical stone is 20 ~ 40mm;
Preferably, the particle diameter of described high molecule microcapsule is 500 ~ 3000 μm;
Preferably, described biological brick is containing, for example the component of lower massfraction: medical stone 10% ~ 35%, fine sand 10% ~ 25%, Water Quenching Slag 10% ~ 20%, pebbles 15% ~ 30%, zeolite 15% ~ 40%;
Preferably, the particle diameter of described pebbles is 30 ~ 60mm;
Preferably, the particle diameter of described medical stone, fine sand, Water Quenching Slag, zeolite is respectively 20 ~ 40mm;
Preferably, in step (5), the preparation process of described biological floating island is: mixed with biological floating island matrix by described complex microorganism, the weight ratio of described complex microorganism and biological floating island matrix is 1:10 ~ 20, be filled in a body, planting aquatic plants, obtain biological floating island;
Preferably, described biological floating island matrix is containing, for example the component of lower massfraction: medical stone 20% ~ 55%, fine sand 15% ~ 25%, Water Quenching Slag 15% ~ 30%, zeolite 25% ~ 65%;
Preferably, the particle diameter of described medical stone, fine sand, Water Quenching Slag, zeolite is respectively 20 ~ 40mm;
Preferably, described waterplant is one or more in reed, Caulis Miscanthis floriduli, Scirpus tabernaemontani, aquatic Canna generalis Bailey, cyperus alternifolius;
Preferably, the planting density of described waterplant is 15 ~ 20 strains/m 2;
Preferably, each biological floating island area is 8 ~ 15m 2;
Preferably, the spacing between adjacent two biological floating islands is 20 meters;
Preferably, in step (6), described hydrocoles comprises silver carp, variegated carp, spiral shell and zooplankton;
Preferably, described silver carp: variegated carp: spiral shell: the weight ratio of zooplankton is 3:1:0.5:0.2.
Wherein, in step (1), screen, cultivate the Rhodopseudomonas spheroides of described complex micro organism fungicide A, the bacillus JY-LZ of complex micro organism fungicide B, the sewage that gushes from the smelly Heihe of target respectively of the sample needed for the organic mineralized bacterium of low temperature of complex micro organism fungicide C and/or bed mud.
Wherein, in step (1), in described complex micro organism fungicide A, the cultivation of spherical Rhodopseudomonas and screening comprise the steps:
(1a) enrichment medium and isolation medium is prepared respectively;
(1b) enrichment culture; The black smelly bed mud 1 ~ 2g in river course getting collection is inoculated in enrichment medium, and liquid level injects sterilising liq paraffin, starvation, forms anaerobic environment, is statically placed in 28 ~ 30 DEG C of cultivations in illumination box, observes at any time; When there is blush grower in culture, getting 0.5 ~ 1.3ml enrichment culture thing from blush grower, being inoculated in another enrichment medium and cultivating; Repeat enrichment culture 2 ~ 3 times;
(1c) be separated through plate streaking successively, shake tube method separation, screen and after slant culture, obtain spherical Rhodopseudomonas;
Preferably, in step (1a), described enrichment medium is containing, for example lower following component: NH 4cl0.8g ~ 1.8g, K 2hPO 40.2 ~ 0.7g, MgSO 40.1 ~ 0.3g, NaCl1.5 ~ 2.5g, yeast extract paste 0.1 ~ 0.3g, NaHCO 31 ~ 5g, dehydrated alcohol 1.5 ~ 2.0ml, liquid microelement 1.0mL;
Preferably, described liquid microelement is containing, for example lower component: H 3bO 30.006 ~ 0.012mg, NaMoO 40.002 ~ 0.008mg, MnSO 40.0005 ~ 0.002mg, ZnSO 40.002 ~ 0.006mg, distilled water constant volume;
Preferably, in step (1a), described isolation medium is containing, for example lower composition: NH 4cl0.8g ~ 1.5g, K 2hPO 40.2 ~ 0.7g, MgSO 40.1 ~ 0.5g, NaCl1.5 ~ 3.0g, yeast extract paste 0.1 ~ 0.3g, NaHCO 31 ~ 3g, dehydrated alcohol 1.5 ~ 2.0ml, agar powder 1.5 ~ 2.0g, Na 2s9H 2o1 ~ 2g.
Compared with prior art, the invention has the advantages that: the microorganism that present method is applied when administering black-odor river comprises three groups of complex micro organism fungicides, these three kinds of mixture microbial inoculums contain three kinds of efficient specificity bacterial classifications---and the organic mineralized bacterium of Rhodopseudomonas spheroides, bacillus JY-LZ and low temperature can adapt to river sewage growing environment, and has specific processing power to river course; These three kinds efficient specificity bacterial classifications and conventional bacterial classification carry out compatibility; form functional effectively compound microbial; in conjunction with various high reactivity matrix and aquatic animal, plants, row comprehensively systematicness process is poured into river, to set up under forming the different stage of economic development China's different geographical around the stream pollution control that water quality function promotes and Ecology protection is target and process for comprehensively treating system.
Apply the administering method that complex microorganism of the present invention carries out gushing in smelly Heihe, step process is polluted by external source, endogenous pollution bed mud advanced treatment and reduction, water ecology is administered and deep purifying, the governance process that water ecology is repaired and regulated and controled, breach the multistage Tiny ecosystem of black smelly water course in situ rebuild and balance the gordian technique of repairing, river is made to gush the strengthening of network type microecosystem and the reconstruction balanced, diversity of organism enriches, water body receive dirt, anti-soil, self-purification capacity and Ecological regulation services increase substantially, more than 10 times can be resisted every year due to typhoon, drain off floodwater, drought resisting is dispatched, tide, the big area substantial pollution that the objective factors such as a large amount of sanitary sewages that heavy rain and burst Pollution impact are introduced cause, water body is impacted in each pollution self-cleaning can reach not black not smelly in 24 hours, return in 48 hours to pollute and impact front water quality standard.Whole water system keeps water quality good, salubrious throughout the year, transparency reaches more than 150cm, and thoroughly eliminate black smelly, bed mud cuts down more than 60cm, water quality reaches country " water environment quality standard " (GB3838-2002) V class standard, and part index number reaches IV class water quality standard.
To sum up, what the administering method that the present invention relates to was set forth is gush multifunctional bio breathing strengthening with river to be reduced to core with internal recycle polluted bed mud, eliminate endogenous pollution and restoration of the ecosystem to black-odor river original position network type multiple-stage treatment and rebuild the internal recycle gushing microecosystem and balance in river strengthen and the new harnessing water idea of ecological cooperative reparation from reducing dispersion pollution of area source, without the need to desilting, without complicated technology and big machinery, toxicological harmless, without waste gas, without dust, non-secondary pollution, there is the feature such as safety, environmental protection, ecology, comprehensive, feasible, efficient, less investment, instant effect.
Accompanying drawing explanation
Fig. 1 is the 1000 Χ light micrographs of the lactic acid producing bacillus successfully screened.
Embodiment
Below in conjunction with accompanying drawing embodiment, the present invention is described in further detail.
Embodiment 1: certain old town moat ecological management and restoration of the ecosystem
This river length of swell about 2600 meters, width average about 15 meters, the depth of water 1.5 ~ 3.0 meters, bottom sludge thickness reaches 0.9 ~ 1.5 meter, because periphery has directly entering of a large amount of sanitary sewage and a small amount of industrial sewage for a long time, could not be processed accordingly again, be caused river water burst body blackout, smelly in the course of time, transparency is 5 centimetres, belongs to serious pollution water body.
This river course whole improvement cycle is 14 months, and concrete preparation method is as follows:
(1) described complex microorganism is prepared; Coupling operating weight ratio between complex micro organism fungicide is: complex micro organism fungicide A: complex micro organism fungicide B: complex micro organism fungicide C=12kg:6.5kg:20kg.
The weight ratio of each bacterial classification of complex micro organism fungicide A is: spherical Rhodopseudomonas: Rhodopseudomonas palustris: capsula Rhodopseudomonas: withered grass bud robe bacillus: bacillus cereus: Bacillus licheniformis: denitrifying bacterium: milk-acid bacteria: yeast: colloid bacillus cereus=4.5kg:2.0kg:3.5kg:2.0kg:2.2kg:2.0kg:2.0kg:1.8kg:1.5kg:2. 1kg.
The weight ratio of each bacterial classification of complex microorganism B is: bacillus JY-LZ: spherical Rhodopseudomonas: bacillus coagulans: bacillus laterosporus: Bacillus licheniformis: milk-acid bacteria: yeast: actinomycetes: azotobacter chroococcum=4.0kg:1.4kg:2.0kg:1.5kg:1.2kg:0.5kg:1.0kg:1.2kg:2.6kg.
The weight ratio of each bacterial classification of complex microorganism C is: the organic mineralized bacterium of low temperature: Rhodopseudomonas palustris: withered grass bud robe bacillus: colloid bacillus cereus: bacillus laterosporus: bacillus pumilus: nitrobacteria: denitrifying bacterium: milk-acid bacteria: yeast: azotobacter chroococcum=6.5kg:2.3kg:1.5kg:5.5kg:1.2kg:1.4kg:1.0kg:2.2kg:1.6kg:0. 9kg:3.1kg.
(2) pre-treatment is carried out to target river course: namely disperse the multiple-stage treatment that external source is polluted, reduce exogenous pollution (lasting 1 month)
Different Biological Pretreatment facility 3 places is designed according to the size of each sewage draining exit and blowdown flow rate, Biological Pretreatment device inside is with biological activity zeolite, high molecule microcapsule embedding, biological medical stone, pebbles and bio-strip for the process of main combined function effectively compound microbial combination technique, and the exogenous pollution reducing high density pollution thing to greatest extent impacts.Undercurrent then main gushes that place arranges to embed bottom anerobe, surperficial aerobic bio-contact oxidation forms biological step treatment zone in conjunction with waterplant bordering on, greatly can reduce the concentration that external source is polluted, alleviate the organic contamination impact load that periphery sewage gushes target, be convenient to follow-up intensive treatment.Gush the biological reinforced band utilizing waterplant and complex microorganism to be formed carry out combination technique improvement, waterplant fraction of coverage 2% with main each of bordering on of gushing, within every 10 days, once usage quantity is 200PPM/ days to complex microorganism.
1, biological reinforced band selection plant is: reed, Caulis Miscanthis floriduli, Scirpus tabernaemontani, aquatic Canna generalis Bailey, cyperus alternifolius, planting density is 20 strains/m 2.
2, sewage draining exit pretreatment unit biologic packing material composition:
1. high molecule microcapsule particle diameter is 1000um; 2. medical stone particle diameter is 50mm; 3. pebbles particle diameter is 50mm; 4. bio-strip: crosslinked with electropositive special soft fiber biologic packing material, Fibre diameter is 25um.
(3) the biological internal recycle respiratory system gushed in river and cometabolism mechanism (lasting 2 months) is built
, water power wretched insufficiency weak according to the characteristic distributions of river polluted thing, water body self-purification ability and the serious phenomenon of ecological degradation are in conjunction with situations such as channel length, water surface form, water (flow) direction, flow velocity and the depth of waters, leading portion respectively in river course, stage casing and back segment arrange that 3 bottom molecule bubble oxygen transmit low-purity oxygen plants, and full river is gushed and added water wheel type surface oxygenating device according to S type method of arranging at interval of 150 meters and carry out the process of combination aeration oxygen replenishing simultaneously.By the regulation and control of systems analysis microbial respiratory, river is gushed to impact and oxygen internal recycle the considering indigenous microorganism cometabolism mechanism of middle microorganism species structure and function, cloth gas disturbance is adopted to promote black smelly bed mud biomineralization and strengthening formula water cycle bio-contact oxidation, greatly can strengthen the water power multiphase flow of upper and lower water body, improve waterpower exchange capacity and hydraulic detention time, increase material conversion and cycle rate, the best original position oxygenation effect of river course entirety can be reached fast, the space flourish of promotion functions effectively compound microbial and morphology control, be conducive to reconstruction and the balance of the multi-functional dominant flora of whole water system.
(4) advanced treatment of polluted bed mud and reduction, balance bed mud structure, sets up river and gushes bottom original position step circular treatment microorganism bed.(elimination endogenous pollution) (lasting 5 months)
Organic contamination bed mud is the important component part of the natural ecosystem of rivers system, the coenosis of bed mud, chemical constitution, chemical form, particularly redox state and biodegradability directly affect the water quality of self-purification capacity and the overlying water gushed in river, are also to cause river to gush black smelly maximum, the most basic source of pollution.It is large that selection has specific surface area, adsorption and sedimentation performance is good, biological group kind is enriched, breeding biofilm is fast, difficult drop-off, the bio-based of the advantage such as low temperature resistant, biological brick, high molecule microcapsule, coordinate functional effectively compound microbial embedding techniques to polluted bed mud bio-oxidation, improve the biodegradability that river is gushed, bed mud organic carbon concentration, the biological respiration rate of regulation and control bed mud and the organic mineralization rate of bed mud carry out the successively minimizing of organic bed mud, within continuous 120 days, evenly to splash in pre-treatment section complex microorganism, microbial inoculum amount is used to be 220PPM/ days, biological carbon (ethanol: sodium acetate=1.5:3) 5PPM, regulate water body C/N than being 7:1, N/P compares 12:1, start porous nano oxygenation facility and surperficial oxygenating device simultaneously.The microbial inoculum usage quantity of later every day and inoculating proportion are according to bed mud changing conditions, discharge and water body (CODcr, NH 3-N, TN etc.) index appropriately adjusts, it is utilize functional effectively compound microbial to carry out enhancing degradation that strengthening Tiny ecosystem is rebuild with balancing technique, the microbiological chain link of each disappearance in polluted river channel Ecology is repaired, build river and gush bottom step cyclic hardening process microorganism bed, useful autochthons is impelled to multiply in a large number, recover diversity of organism, promote diversification and abundantization of the metabolism of bottom mud microbe and polluted bed mud layer bottom-dwelling population, thus the organic pollutant reduced fast in mud, significantly cut down polluted bed mud.This stage river gushes that basically eliminate is black smelly, and bed mud cuts down about 40 centimeters, and after sediment pollution is progressively cut down, bed mud thickly becomes loose shape by original, and color becomes natural yellow by ink black, and organic pollutants in water body declines more than 60%.
1. the component of bio-based weight fraction: medical stone 30%, high molecule microcapsule 70%. medical stone particle diameter are 30mm, and high molecule microcapsule particle diameter is 1000um; 2. the component of biological brick weight fraction: medical stone 45%, Water Quenching Slag 10%, zeolite 45%; The particle diameter of component is 30mm.
(5) ecological management of water body and deep purifying, namely river is gushed microecosystem and is rebuild and balance (4 months)
Gush in river on the basis that organic contamination bed mud is significantly cut down and microorganism bed is set up, in conjunction with waterplant and biological floating island according to discharge and sewage (CODcr, NH 3-N, TN etc.) index suitably adjusts microbial inoculum proportioning and dosage, continues every day to add complex microorganism, uses microbial inoculum amount to be 180PPM, regulates water body C/N than for 5:1, N/P are than being 20:1.Interbiotic synergy and three nitrogen cycles are utilized to carry out nitrated to organic pollutant, denitrification, short-cut denitrification, fixed nitrogen, separate sulphur, poly-phosphorus, the biochemical reactions such as phosphorus decomposing, significantly reduce high concentration organic contaminant, three-dimensional lifting water quality index, optimize biocenological number ratio, on the basis ensureing species diversity, further adjustment beneficial microorganism population, improve and stabilizing water quality, thus form perfect food chain circulation and healthy microecosystem, strengthen the Ecological regulation services of water body, self-purification capacity and material cycle, rebuild the microecosystem and balance that gush in river, the ecosystem function of the Nature finally realize increasing water quality index and water body is utilized to become clear, become the target of living, it is black smelly that thoroughly elimination is gushed in this stage river, water body becomes canescence from black and finally transfers light green to, bed mud is cut to about 60 centimeters, organic pollutants in water body declines more than 85%.
1. the making method of biological floating island is: mixed with matrix by above-mentioned complex microorganism (weight ratio is 1:15), be filled in a body according to water (flow) direction sectional type, and in the implantation hole in online face, bed body top planting aquatic plants, Scirpus tabernaemontani, aquatic Canna generalis Bailey, cyperus alternifolius, planting density is 15 strains/m 2.Each biological floating island area is 10m 2, the spacing between adjacent two chinampa unit is 20 meters.
2. the component of biological floating island matrix weight mark: medical stone 40%, fine sand 15%, Water Quenching Slag 15%, zeolite 30%; The particle diameter of four kinds of components is 40mm.
3. the first inoculating complex microorganism of submerged plant, then use fork planting method design arrangement, fraction of coverage be generally that area is gushed in target river 6%.Select based on the submerged plant of watermifoil, eel grass.
4. emergent: based on efficient soil pick-up, gushes apart from river within the scope of the 1m of two sides and plants, beat bamboo timber to reach planting conditions when sloping bank does not meet planting conditions, and water is crossed in dark scope and adopted artificial floating island plantation emergent.Selection plant is: blend proportion is the reed of 0.5:3:1:0.5:1: Caulis Miscanthis floriduli: Scirpus tabernaemontani: aquatic Canna generalis Bailey: cyperus alternifolius, and planting density is 15 strains/m2.
(6) water ecology reparation and multi-functional regulation and control (build aquatic animal, plants group, improve water quality) (2 months)
After the microecosystem function of water body and Equilibrium fitting, according to discharge and COD of sewage cr, NH 3the index such as-N, TN suitably adjusts complex microorganism proportioning and dosage, adds complex microorganism every 7 days, uses microbial inoculum amount to be 50PPM, and regulating water body C/N than being 5:1, N/P ratio is 10:1.Functional effectively compound microbial engineering is utilized to repair and original position multi-stage ecological purification technology, in conjunction with hydrocoles control measures, put hydrocoles in a suitable place to breed, extend ecological chain, have additional nutrients the output pathway of salt, improve material cycle transformation efficiency, the bacterium phase of balance and stability aquatic ecosystem, aqueous phase and algae phase, ensure biocenological number ratio and diversity of organism, the regulatory function of comprehensive restored ecosystem, strengthen gush in river anti-soil, receive dirt and self-purification capacity, promote water-quality guideline, improve Water quality and overall sense organ.
Type of aquatic is: silver carp, variegated carp, spiral shell and zooplankton.Per kilogram hydrocoles control 100m 2water area, per kilogram is according to silver carp: variegated carp: spiral shell: zooplankton=3:1:0.5:0.2.
Regulation effect is gushed in table 1 ~ table 3 in the present embodiment river.
Table 1: changing conditions unit: mg/L before and after the improvement of every contamination index is gushed in river
Table 2: organic sludge specific index and height measurement results are gushed in river
Table 2-1
Table 2-2
Table 3: the test result unit of water body self-purification ability: mg/L after administering
The present embodiment is administered front and back water-quality guideline and is entrusted environment protection and monitoring station carry out water quality monitoring (WQM) and provide this data.From table 1 ~ table 3, by the improvement of this technology, the microecosystem that river is gushed is rebuild with balance, Ecological regulation services and the restoration of the ecosystem ability of water body are strengthened greatly, its organic pollutant all shows obvious clearance, successful, river water quality all reaches country " water environment quality standard " (GB3838-2002) V class standard, and part index number reaches IV class water quality standard.Wherein bed mud respiratory capacity rises to 248.17mgC.m from original 108.64 -2.d -1, Sediment bacteria number is multiplied to 15.35*109Cells.g by original 0.87*107 -1, bed mud thickness on average have dropped 60cm, and the average removal rate of COD, BOD5, ammonia nitrogen, total phosphorus is 87.9%, 80.0%, 94.8%, 90.3% respectively, and dissolved oxygen has risen to 5.2mg/L from 0.4.
Embodiment 2: certain the natural ecosystem of rivers is administered and restoration of the ecosystem
River length of swell about 4200 meters, width average about 12 meters, depth of water 1.5-2.0 rice, river is gushed the black smelly silt depth in bottom and is reached 1.0 ~ 1.5 meters.Due to directly entering of the sewage such as Residential areas, retail shop, food and drink along the river, this gush become in order to one integrate blowdown, the sewage river course of function of controlling flood and draining flooded fields, cause water body stench, transparency is almost nil, the a large amount of scum silica frost of floating on water, animal oil and rubbish, the shinny blackout of water body color, ecological functions and balance are destroyed completely, and river is gushed and lost self-purification capacity.
This river course whole improvement cycle is 14 months, and concrete governance process is as follows:
(1) complex microorganism is prepared; Complex micro organism fungicide A: complex micro organism fungicide B: complex micro organism fungicide C=14kg:8kg:26kg.
The weight ratio of each bacterial classification of complex micro organism fungicide A is: spherical Rhodopseudomonas: Rhodopseudomonas palustris: capsula Rhodopseudomonas: withered grass bud robe bacillus: bacillus cereus: Bacillus licheniformis: denitrifying bacterium: milk-acid bacteria: yeast: colloid bacillus cereus=5.8kg:2.0kg:2.6kg:3.0kg:2.8kg:2.6kg:2.5kg:2kg:1.5kg:1.6k g.
The weight ratio of each bacterial classification of complex micro organism fungicide B is: bacillus JY-LZ: spherical Rhodopseudomonas: bacillus coagulans: bacillus laterosporus: Bacillus licheniformis: milk-acid bacteria: yeast: actinomycetes: azotobacter chroococcum=4.2kg:1.5kg:2.5kg:1.4kg:1.6kg:0.8kg:1.2kg:1.0kg:1.8kg.
The weight ratio of each bacterial classification of complex micro organism fungicide C is: the organic mineralized bacterium of low temperature: Rhodopseudomonas palustris: withered grass bud robe bacillus: colloid bacillus cereus: bacillus laterosporus: bacillus pumilus: nitrobacteria: denitrifying bacterium: milk-acid bacteria: yeast: azotobacter chroococcum=8.5kg:1.8kg:3.5kg:4.5kg:1.9kg:1.6kg:2.5kg:2.6kg:1.4kg:1. 0kg:2.4kg.
(2) carry out Biological Pretreatment to target river course, dispersion external source pollutes multiple-stage treatment.(reduction exogenous pollution) (1 month)
The distinctive points of this step and embodiment 1 is: on the one hand, designs different Biological Pretreatment facility 6 places according to the size of each sewage draining exit and blowdown flow rate; On the other hand, within every 7 days, once usage quantity is 250PPM/ days to complex microorganism.The other guide of this step is with reference to embodiment 1.
(3) the biological internal recycle respiratory system gushed in river and cometabolism mechanism (2 months) is built
This step is with reference to embodiment 1.
(4) advanced treatment of polluted bed mud and reduction, balance bed mud structure, sets up river and gushes bottom original position step circular treatment microorganism bed.(elimination endogenous pollution) (5 months)
This step difference from Example 1 is: first, and the usage quantity of complex microorganism is 300PPM/ days, simultaneously biological carbon (ethanol: sodium acetate=1.5:3) 3PPM, and regulating water body C/N than being 7:1, N/P ratio is 12:1; Secondly, the component of bio-based weight fraction: medical stone 40%, high molecule microcapsule 60%. medical stone particle diameter are 30mm, and high molecule microcapsule particle diameter is 1000um; Then, the component of biological brick weight fraction: medical stone 30%, fine sand 10%, Water Quenching Slag 10%, pebbles 10%, zeolite 40%; Pebbles particle diameter is 40mm, and the particle diameter of other component is 30mm.
The other guide of this step is with reference to embodiment 1.
(5) water ecology is administered and deep purifying.(river is gushed microecosystem and rebuild and balance) (4 months)
This step difference from Example 1 is: first, and the injected volume of complex microorganism is 280PPM/ days; In addition, this stage river is gushed and is thoroughly eliminated black smelly, and water body becomes canescence from black and finally transfers light green to, and bed mud is cut to about 60 centimeters, and organic pollutants in water body declines more than 90%.Secondly, the component of biological floating island matrix weight mark: medical stone 50%, fine sand 10%, Water Quenching Slag 10%, zeolite 30%; The particle diameter of four kinds of components is 40mm.Finally, emergent: reed: Caulis Miscanthis floriduli: Scirpus tabernaemontani: aquatic Canna generalis Bailey: the blend proportion of cyperus alternifolius is 1:2:0.5:0.5:2;
Other of this step are with reference to embodiment 1.
(6) water ecology reparation and multi-functional regulation and control.(build aquatic animal, plants group, improve water quality) (2 months)
This step difference from Example 1 is: the injected volume of complex microorganism is 80PPM/ days.Other are as embodiment 1.
The mass effect in the present embodiment river course is in table 4 ~ table 6.
Table 4: changing conditions unit: mg/L before and after the improvement of every contamination index is gushed in river
Table 5: organic sludge specific index and height measurement results are gushed in river:
Table 5-1:
Table 5-2:
Table 6: the test result of water body self-purification ability after administering: unit: mg/L
Before and after this engineering control, water-quality guideline entrusts environment protection and monitoring station carry out water quality monitoring (WQM) and provide this data.As can be known from the above table, by the improvement of this technology, many and receive dirty situation without in considerable change situation at pollution of river thing, dirty situation of wherein receiving refers to that river course is to newly entering the oxidation rate of pollutent, dynamics and self-purification capacity, water pollutant all shows obvious clearance, successful, river water quality all totally reaches country " water environment quality standard " (GB3838-2002) V class standard, and part index number reaches IV class water quality standard.Wherein bed mud respiratory capacity rises to 268.15mgC.m from original 95.33 -2.d -1, Sediment bacteria number is multiplied to 21.41*109Cells.g by original 0.96*107 -1, bed mud thickness on average have dropped 57cm, and the average removal rate of COD, BOD5, ammonia nitrogen, total phosphorus is 89.4%, 85.1%, 95.5%, 91.7% respectively, and dissolved oxygen has risen to 5.5mg/L from 0.1.
Embodiment 3: certain urban waterway ecological management and restoration of the ecosystem
Total length 2.4km is gushed in this river, river surface maximum width 30m, minimum width 10m, average depth 2.8m, gushes the underground river Zhi Yongchang be connected be about 4km with this.Add up according to Field Research, this gushes bank and comprises sanitary sewage, restaurant wastewater, animal cultivation waste water, the in line sewage of trade effluent septic tank etc., about have every day the sewage of 1.5 ten thousand tons to be gushed by sewage draining exit and tens of little undercurrents and ramuscule to enter main river and gush (draining every day (fine day) scale according to sluice mouth calculates), rainy day, the sewage amount of entering reached more than 15 ~ 200,000 tons (calculating according to riverbank scale data), all sewage is pooled to after this master gushes, and enters the Zhujiang River through sluice.Because long-term accumulation causes water burst face, river to be covered with suspended substance less than process in time again, bed mud blackout is smelly, organic sludge thickness about 1.7 ~ 2 meters, water body produces strong foul smell, dissolved oxygen is zero, and transparency is zero, and the eubiosis is destroyed completely, river is gushed and is lost self-purification capacity, has a strong impact on Zhujiang River water quality and nearby residents life.
This river course whole improvement cycle is 14 months, and concrete governance process is as follows:
(1) described complex microorganism is prepared: complex micro organism fungicide A: complex micro organism fungicide B: merit complex micro organism fungicide C=20kg:10kg:30kg.
The weight ratio of each bacterial classification of complex micro organism fungicide A is: spherical Rhodopseudomonas: Rhodopseudomonas palustris: capsula Rhodopseudomonas: withered grass bud robe bacillus: bacillus cereus: Bacillus licheniformis: denitrifying bacterium: milk-acid bacteria: yeast: colloid bacillus cereus=6.4kg:2.5kg:4.0kg:3.5kg:2.4kg:2.6kg:2.5kg:2.2kg:2.5kg:3. 0kg.
The weight ratio of each bacterial classification of complex micro organism fungicide B is: bacillus JY-LZ: spherical Rhodopseudomonas: bacillus coagulans: bacillus laterosporus: Bacillus licheniformis: milk-acid bacteria: yeast: actinomycetes: azotobacter chroococcum=4.8kg:1.5kg:3.8kg:3.4kg:1.6kg:0.8kg:1.2kg:1.0kg:2.2kg.
The weight ratio of each bacterial classification of complex micro organism fungicide C is: the organic mineralized bacterium of low temperature: Rhodopseudomonas palustris: withered grass bud robe bacillus: colloid bacillus cereus: bacillus laterosporus: bacillus pumilus: nitrobacteria: denitrifying bacterium: milk-acid bacteria: yeast: azotobacter chroococcum=6.5kg:3.6kg:4.8kg:6.0kg:2.3kg:1.6kg:3.2kg:3.6kg:2.2kg:2. 0kg:1.9kg.
(2) carry out Biological Pretreatment to target river course, dispersion external source pollutes multiple-stage treatment.(reduction exogenous pollution) (1 month)
This step difference from Example 1 is: on the one hand, designs different Biological Pretreatment facility 10 places according to the size of each sewage draining exit and blowdown flow rate; On the other hand, within every 3 days, once usage quantity is 280PPM/ days to functional effectively compound microbial inoculant.The other guide of this step and operation are with reference to embodiment 1.
(3) the biological internal recycle respiratory system gushed in river and cometabolism mechanism (2 months) is built
This step is with reference to embodiment 1.
(4) advanced treatment of polluted bed mud and reduction, balance bed mud structure, sets up river and gushes bottom original position step circular treatment microorganism bed.(elimination endogenous pollution) (5 months)
This step difference from Example 1 is: first, complex microorganism of evenly splashing in pre-treatment section for continuous 120 days, and injected volume is 320PPM/ days, biological carbon (ethanol: sodium acetate=1.5:3) 3.8PPM, regulates water body C/N than for 6:1, N/P are than being 10:1; Secondly, bed mud cuts down about 45 centimeters, and after sediment pollution is progressively cut down, bed mud thickly becomes loose shape by original, and color becomes natural yellow by ink black, and organic pollutants in water body declines more than 65%.Then, the component of bio-based weight fraction: medical stone 40%, high molecule microcapsule 60%. medical stone particle diameter are 30mm, and high molecule microcapsule particle diameter is 1000um; Then, the component of biological brick weight fraction: medical stone 30%, fine sand 10%, Water Quenching Slag 10%, pebbles 10%, zeolite 40%; Pebbles particle diameter is 40mm, and the particle diameter of other component is 30mm.
All the other operations of this step and content are with reference to embodiment 1.
(5) water ecology is administered and deep purifying.(river is gushed microecosystem and rebuild and balance) (4 months)
This step is with the difference of embodiment 1: first, the injected volume of complex microorganism is 300PPM/ days, and regulating water body C/N than being 5:1, N/P ratio is 18:1; Secondly, bed mud is cut to about 63 centimeters, and organic pollutants in water body declines more than 86%.Then, the component of biological floating island matrix weight mark: medical stone 55%, fine sand 5%, Water Quenching Slag 10%, zeolite 30%; The particle diameter of four kinds of components is 40mm.Then, the fraction of coverage of submerged plant is that 4% of area is gushed in target river; Finally, the reed of emergent to be blend proportion be 1:1:0.5:0.5:2: Caulis Miscanthis floriduli: Scirpus tabernaemontani: aquatic Canna generalis Bailey: cyperus alternifolius.
All the other contents of this step and operation are with reference to embodiment 1.
(6) water ecology reparation and multi-functional regulation and control.(build aquatic animal, plants group, improve water quality) (2 months)
This step difference from Example 1 is: on the one hand, and complex microorganism added every 5 days, and dosage is 100PPM/ days, and regulating water body C/N than being 5:1, N/P ratio is 11:1.On the other hand, per kilogram is according to silver carp: variegated carp: spiral shell: zooplankton=2.5:1:0.5:0.3.
The regulation effect in the present embodiment river course is in table 7 ~ table 9.
Table 7: changing conditions unit: mg/L before and after the improvement of every contamination index is gushed in river
Time index Before improvement Administer after 3 months Administer after 6 months Administer after 10 months Operative norm
PH value 4.6 6.3 6.7 7.0
DO 0.0 2.5 4.1 5.9 GB7489-87
NH3-N 47.7 26.5 17.4 2.4 GB7479-87
COD 415 217 135 39 GB11914-89
BOD5 60 42 25 9.6 GB7488-87
TP 5.52 3.12 2.11 0.48 GB11893-89
Table 8: organic sludge specific index and height measurement results are gushed in river:
Table 8-1:
Table 8-2:
Section or sampling point Reference surface Before engineering construction (m) After engineering construction (m) Reduce height (cm)
1 Vertical river levee fixed face 1.63 2.22 59
2 Vertical river levee fixed face 2.15 2.80 65
3 Vertical river levee fixed face 1.95 2.56 61
Table 9: the test result unit of water body self-purification ability: mg/L after administering
Period index Before big area Sewage shock In Sewage shock Impact latter 24 hours Impact latter 48 hours
Water body sense organ Water body light green, odorless Water body black, has strong stink Water body deep green, basic odorless Water body light green, odorless
COD 40 98.5 59.68 40.5
BOD 5 9.5 67.8 32.9 10.4
NH 3-N 2.17 9.44 4.72 2.10
TP 0.43 4.03 1.26 0.44
Before and after the present embodiment river regulation, water-quality guideline entrusts environment protection and monitoring station carry out water quality monitoring (WQM) and provide this data.As can be known from the above table, by the improvement of this technology, the microecosystem that river is gushed is rebuild with balance, Water quality General Promotion, had extremely strong dirt and the self-purification capacity received, at the pollution of river thing when many and lasting blowdown, water pollutant all shows significant clearance, successful, river water quality totally reaches country " water environment quality standard " (GB3838-2002) V class standard. and wherein bed mud respiratory capacity rises to 317.45mgC.m from original 82.08 -2.d -1, Sediment bacteria number is multiplied to 26.02*109Cells.g by original 0.48*107 -1, bed mud thickness on average have dropped 61cm, and the average removal rate of COD, BOD5, ammonia nitrogen, total phosphorus is 90.6%, 84.0%, 95.0%, 91.3% respectively, and dissolved oxygen has risen to 5.9mg/L from 0.1.
When the organic mineralized bacterium of low temperature that the bacillus JY-LZ that the Rhodopseudomonas spheroides that above-described embodiment complex micro organism fungicide A contains, complex micro organism fungicide B contain, complex micro organism fungicide C contain is cultivated, sewage and/or the mud of black-odor river taken from respectively by required sample.Specifically describe the concrete culturing process of the organic mineralized bacterium of Rhodopseudomonas spheroides, bacillus JY-LZ and low temperature below.
(1) Rhodopseudomonas spheroides (Rhodopseudornonaspalustris)
1. materials and methods
1.1 bacterial classification sources
The sample gathered is target canal reactor and organic bed mud, altogether acquires 6 local samples.
1.2 substratum:
1. the composition of enrichment medium is: NH 4cl1.5g, K 2hPO 40.3g, MgSO 40.2g, NaCl2.1g, yeast extract paste 0.25g, NaHCO 32.5g, dehydrated alcohol 2.0ml, liquid microelement 1.0mL, add distilled water 900ml stir after in 121 DEG C of sterilizings 20 minutes, then add sterilized somatomedin 1.2ml, pH to 6.8 ~ 7.0;
Wherein, liquid microelement is containing, for example lower component: H 3bO 30.009mg, NaMoO 40.005mg, MnSO 40.001mg, ZnSO 40.004mg, distilled water constant volume.
2. the composition of isolation medium is: NH 4cl1.0g, K 2hPO 40.5g, MgSO 40.35g, NaCl1.8g, yeast extract paste 0.25g, NaHCO 31.5g, dehydrated alcohol 2.0ml, agar powder 1.5g, Na 2s9H 2o1.5g, distilled water 900ml, in 121 DEG C of sterilizings 20 minutes after stirring, then add sterilized vitamin H 0.002mg, adjusts pH to 6.8 ~ 7.0;
3. enrichment culture: the black smelly bed mud 1 ~ 2g in river course getting collection is inoculated in enrichment medium, liquid level injects sterilising liq paraffin (height is 2cm about), starvation, forms anaerobic environment, is statically placed in 28 ~ 30 DEG C of cultivations in illumination box, observes at any time; When there is blush grower in culture, getting 1ml enrichment culture thing from blush grower, being inoculated in another enrichment medium and cultivating; Repeat enrichment culture 2 ~ 3 times;
4. plate streaking is separated: get enrichment culture thing and carry out plate streaking separation, be inoculated in isolation medium, substratum covers sterilising liq paraffin, starvation, cultivate 6 ~ 10 days for 28 ~ 30 DEG C in illumination box, observe microscopy, the redness list bacterium colony that picking motion is active, repeat plate streaking to be separated 3 ~ 4 times, to obtain pure bacterial strain;
5. shake tube method to be separated: select the pure bacterial strain that obtains of line partition method, do percutaneous puncture-inoculation, substratum is isolation medium, the wax sealing of mouth of pipe jam-pack, intensity of illumination be 500 ~ 20001ux, temperature is 25 ~ 32 DEG C, cultivate 4 ~ 7 days under anaerobic condition;
6. screen: bacterial strain is obtained to separation and tentatively selects, choose the comparatively faster bacterial strain of the speed of growth.
7. slant culture: first adopt slant medium to transfer the bacterial strain separated, dark aerobic 28 ~ 30 DEG C are trained first class inoculum, again first class inoculum is inoculated in first class inoculum substratum, second class inoculum is trained by 100ml culturing bottle illumination anaerobism 28 ~ 30 DEG C, second class inoculum is inoculated in second class inoculum substratum, three-class strain is trained by 500ml culturing bottle illumination anaerobism 28 ~ 30 DEG C, again three-class strain and sterilized water are mixed in the ratio of 1:10, be inoculated in three-class strain substratum, be sub-packed in the transparent drum of 5L, be placed in outdoor, cultivate under natural light condition, in cultivating process, controlled light intensity is 500 ~ 20001ux, temperature is 25 ~ 32 DEG C, cultivate and become level Four bacterial classification in 3 ~ 4 days, bacterium number is about 6.5*109 ~ 12.6*109,
8. 1, slant culture based component is: NH 4cl0.8g ~ 1.8g (preferred 1.0g), K 2hPO 40.2 ~ 0.7g (preferred 0.35g), MgSO 40.1 ~ 0.3g (preferred 0.16g), NaCl1.5 ~ 2.5g (preferred 1.26g), somatomedin 0.2 ~ 3.0mL (preferred 1.5mL), para-amino benzoic acid 0.05 ~ 0.15mg (preferred 0.08mg), yeast extract paste 0.1 ~ 0.3g (preferred 0.3g) and distilled water 900ml, in 121 DEG C of sterilizings 20 minutes after stirring, then add sterilized 100mL30g/LNaHCO 3with 2.0ml dehydrated alcohol, regulate pH to 6.8 ~ 7.0(preferably 6.8);
2, the medium component of first class inoculum and second class inoculum is: NH 4cl0.8g ~ 1.8g (preferred 1.25g), K 2hPO 40.2 ~ 0.7g (preferred 0.5g), MgSO 40.1 ~ 0.3g (preferred 0.24g), NaCl1.5 ~ 2.5g (preferred 2.06g), para-amino benzoic acid 0.1 ~ 0.3mg (preferred 0.1mg), biological chelated iron 0.4 ~ 1.5mg (preferred 1mg), yeast extract paste 0.1 ~ 0.3g (preferred 0.3g), NaHCO 34 ~ 5g (preferred 4.0g) and distilled water 900ml, in 121 DEG C of sterilizings 20 minutes after stirring, then adds sterilized 100mL50g/LNaHCO 3with dehydrated alcohol 1.5-2.0ml (preferred 2.0ml), regulate pH to 6.8 ~ 7.0(preferably 6.8);
3, the medium component of three-class strain and level Four bacterial classification is: sodium acetate (three hydrations) 3 ~ 7.0g (preferred 6.0g), ethanol 2.5 ~ 8.5mL (preferred 5.5mL), ammonium chloride 1.3 ~ 3.5g (preferred 1.3g), sodium bicarbonate 3.2 ~ 4.9g (preferred 3.85g), sodium hydroxide 1.0 ~ 2.5 (preferred 1.8g), magnesium chloride 0.3 ~ 0.6g (preferred 0.46g), potassium primary phosphate 3 ~ 8.3g (preferred 4.65g), sodium-chlor 0.2 ~ 0.6g (preferred 0.3g) and calcium chloride 0.02 ~ 0.10g (preferred 0.08g) (separately sterilizing), Sodium Propionate 0.2 ~ 1.6g (preferred 0.8g), sodium sulfate 0.02 ~ 0.05g (preferred 0.03g), yeast extract paste 0.15 ~ 0.9g (preferred 0.5g), biological chelated iron 1 ~ 5.5mg (preferred 4.2mg), para-amino benzoic acid 1 ~ 6g (preferred 4.5g), peptone 0.2 ~ 0.8g (preferred 0.45g), 1000mL water, somatomedin 0.2 ~ 5.0mL (preferred 3.0mL), L-glutamic acid 0.05 ~ 0.32g (preferred 0.065g), compound VB0.6 ~ 2.5 (preferably 1.6), liquid microelement 1mL, its trace element contained and concentration are Fe 2+0.02 ~ 0.06Mg/L (preferred 0.042Mg/L), Cu 2+0.01 ~ 0.05Mg/L (preferred 0.02Mg/L), H 3bO 30.04 ~ 0.08Mg/L (preferred 0.043Mg/L), Mn 2+0.01 ~ 0.05Mg/L (preferred 0.028Mg/L), Zn 2+0.05 ~ 0.1Mg/L (preferred 0.085Mg/L) and Co 2+0.2 ~ 0.8Mg/L (preferred 0.06Mg/L), the pH value of substratum is 6.8 ~ 7.5,
9. screen: with reference to the cell shape of demand microorganism itself, culture color, filter out that activity is relatively high, bacterial strain more targetedly further, and carry out test for identification.
10. strain identification: carry out test for identification according to " uncle Jie Shi Bacteria Identification handbook " the 8th edition through enlarged culturing, pass through morphologic observation, the cell shape of reference Rhodopseudomonas palustris bacterial classification and spherical Rhodopseudomonas bacterial classification and culture color, carry out utilization of carbon source experiment.336 strain photosynthetic bacteriums are isolated in this test altogether, and wherein have 29 strains after slant strains is transferred to 100ml drip cylinder liquid nutrient medium two days to three days, nutrient solution starts to redden, and all the other then need more than three days.According to the growth curve of this 29 strain bacterial strain in further enlarged culturing process, and cell shape, bacterial classification source and culture color, filter out 12 strain bacterial classifications, by 12 strain bacterial classifications of enlarged culturing, bacteria suspension is made with stroke-physiological saline solution washing thalline, be seeded in the isolation medium of following interpolation different carbon source, cultivate 48h, observe the metabolic characteristic of bacterial classification, activity is filtered out further relatively high from the bacterial classification of enlarged culturing, bacterial classification more targetedly, this test finally filters out 2 strain bacterial classifications, bacterial classification HD-1 and bacterial classification HL-20, this two strains bacterial classification all can not utilize elementary sulfur as photosynthetic electron donor, the different oxygen of luminous energy, facultative aerobic, Rhodopseudomonas palustris bacterial classification and spherical Rhodopseudomonas bacterial classification is tentatively defined as through identifying, identification mark is as shown in table 10:
Table 10:
In table 10, "+", for utilizing, "-" is for utilizing.
According to the physiological and biochemical property of above-mentioned two kinds of bacterial strains, tentatively determine that above-mentioned bacterial strains HD-1 is swamp Rhodopseudomonas (Rhodopseudoruonczssphaeroides), bacterial strain HL-20 is spherical Rhodopseudomonas (Rhodopseudornonaspalustris), is classified as photosynthetic bacterium and belongs to (Photosyntheticbacteria).
(2) the organic mineralized bacterium of low temperature (organicBacillaceae)
Special psychrophile, the bacterization in contaminated environment and growth, then contribute to improving the biodegradability to difficult chemical substance.Because these microorganisms have high catalytic activity enzyme and their special specificitys under lower optimal temperature, psychrophile is made to become the ideal tools of biological treating.
Select sampling position according to the growth characteristics of psychrophile, the sample of collection is bed mud, and target river is gushed and altogether acquired 5 local samples.The cultivation of the organic mineralized bacterium of the present invention's low temperature used and discriminating, with reference to prior art.
Such as, the formula of isolation medium is: extractum carnis 5g, peptone 10g, sodium-chlor 5g, pH7.2 ~ 7.4, distilled water 1000ml, and solid medium adds the agar of 1.5% ~ 2%.
The formula producing gemma substratum is: broth culture: 100ml distilled water, Semen Maydis powder 5g, yeast extract paste 0.5 ~ 1g, pH7.2 ~ 7.4, the agar of solid medium 1.5% ~ 2%.
The preparation process of sample diluting liquid is: accurately take testing sample 10g, puts into and 90ml sterilized water is housed and the 250ml triangular flask being placed with little granulated glass sphere, puts 20min that shaking table vibrates, microorganism cells is disperseed, and leaves standstill 20 ~ 30s, 10-1 diluent; Use the aseptic rifle head of 1ml again, draw 10-1 diluent 1ml, move into and be equipped with in the test tube of 9ml sterilized water, pressure-vaccum 3 times, allows bacterium liquid mix, 10-2 diluent; Change an aseptic rifle head again and draw 10-2 diluent 1ml, move into and be equipped with in the test tube of 9ml sterilized water, pressure-vaccum 3 times, also allows bacterium liquid mix, 10-3 diluent; The like, serial dilution, makes a series of extent of dilution such as 10-4,10-5,10-6,10-7,10-8.
The screening of the plating cultivation of the organic mineralized bacterium of low temperature, the purifying of picking list bacterium colony, bacterial classification and preservation, bacterial strain and qualification, with reference to prior art.
(3) lactic acid producing bacillus (B.lacticacidbacteria)
1. materials and methods
1.1 samples: pick up from 3 parts, black-odor river bed mud sample.
1.2 substratum:
(1) modified MRS culture medium: α-methylglucoside (8 ~ 15g) is 10.0g preferably, yeast extract (4 ~ 8g) is 5.0g preferably, Shi peptone (6 ~ 13g) is 10.0g preferably, meat medicinal extract (3 ~ 8g) is 5.0g preferably, sodium acetate (4 ~ 7g) is 5.0g preferably, ammonium citrate (1 ~ 4g) is 2.0g preferably, Sodium phosphate dibasic (1 ~ 3g) is 2.0g preferably, potassium sorbate (0.6 ~ 1.3g) is 1.0g preferably, magnesium sulfate (0.05 ~ 0.2g) is 0.1g preferably, tetrabromo-mcresolsulfonphthalein (0.02 ~ 0.028g) is 0.0224g preferably, manganous sulfate (0.02 ~ 0.06g) is 0.05g distilled water 1000mL preferably, pH5.5.
(2) YPD substratum: glucose (15 ~ 28g) is 20g preferably, yeast extract (6 ~ 15g) is 10g preferably, and peptone (16 ~ 30g) is 20g preferably, distilled water 1000mL, pH nature.
(3) sour screening culture medium is produced: glucose (15 ~ 26g) is 20g preferably, yeast extract (8 ~ 13g) is 10g preferably, and peptone (18 ~ 25g) is 20g preferably, and light calcium carbonate (4 ~ 6.5g) is 5g preferably, distilled water 1000mL, pH nature.
The isolation and screening of 1.3 lactic acid producing bacilluss
Take 5g mud sample and sterilely put into 100mL triangular flask, add 50mL modified MRS culture medium wherein.Adjust pH to 5.5 with 1mol/L acetic acid, soil supension leaves standstill 5min after intense oscillations, is then gone to by supernatant in an aseptic 100mL triangular flask, with 1mol/L acetic acid or NaOH readjustment pH to 5.5.Substratum is placed in 37 DEG C to cultivate 7 days, then get 2mL sample at 80 DEG C of process 20min, the sample getting 0.1mL process is applied to modified MRS agar plate, cultivates 72h, chooses the bacterium colony of growth for 37 DEG C, and line purifying is cultivated, and is placed in 4 DEG C and saves backup.
Dibbling is in the sour screening culture medium of product respectively separation to be obtained bacterial strain, and 37 DEG C of cultivation 24h, screen the bacterial strain that transparent circle is larger.
The qualification of 1.4 lactic acid producing bacilluss
1.4.1 Observation On The Morphology and bio-chemical characteristics
Bacillus C11 is inoculated in after LB substratum 37 DEG C cultivates 24h and observes colonial morphology, optical microphotograph Microscopic observation gramstaining thalli morphology.With reference to uncle Jie Shi handbook the 8th edition, bio-chemical characteristics is carried out to bacterial strain C11, as sugar-fermenting, Starch Hydrolysis, indole test, methyl red test, VP reaction etc.
1.4.216SrDNA the pcr amplification of gene segment and sequential analysis
According to the method Isolation and purification bacteria total DNA in " Molecular Cloning: A Laboratory guide " (third edition).
Design two PCR primer to be respectively
P0：5’-GAGTTTGATCMTGGCTCAG-3’
PC3：5’-CTAHAGGGTATCTAATCCT-3’。
PCR program setting is: 95 DEG C of 5min, 94 DEG C of 1min, 50 DEG C of 1min, 72 DEG C of 1min, 30 rear 72 DEG C of extension 10min of circulation.
The recovery purifying of PCR primer, carries out the mensuration of DNA sequence dna.16SrDNA sequence in measured sequence and Genbank is carried out com-parison and analysis.
The research of 1.5 lactic acid producing bacillus biological characteristicses
1.5.1 the mensuration of growth curve and pH curve
Experimental strain is with 1% inoculum size access 200mLYPD liquid nutrient medium, 37 DEG C of 200rpm shaking culture 24h, and measuring OD600nm and the pH value of nutrient solution every 2h, is that X-coordinate makes growth curve and pH curve with time.
1.5.2 the mensuration of salt resistance ability
In YPD liquid nutrient medium, add NaCl, make its massfraction be respectively 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, be sub-packed in PA bottle, every bottle of 5mL.The bacterial strain activated is inoculated in above-mentioned substratum with 1% inoculum size, 37 DEG C of constant temperature culture 24h, observes growth conditions.
1.5.3 the mensuration of bile tolerance ability
In YPD liquid nutrient medium, add Iocholic acid salt, make its massfraction be respectively 0.15%, 0.2%, 0.25%, 0.3%, 0.4%.The same 1.5.2 of following steps.
1.5.4 the mensuration of resistance
The microbiotic of experiment adopts commercially available drug sensitive test paper.With aseptic glass rod, bacterium liquid is evenly coated on LB flat board, absorbs the various antibacterial scraps of paper, be close to media surface respectively, be placed in 37 DEG C of incubators and cultivate 24h, measure the diameter of inhibition zone.Diameter 15 ~ 20mm is Gao Min, and diameter 10 ~ 15mm is quick in being, below diameter 10mm is muting sensitive, is insensitive without bacterial restrain.
2. results and analysis
The isolation and screening of 2.1 lactic acid producing bacilluss
From pedotheque, separating, selecting obtains bacillus 25 strain, and number consecutively is C1-C25.Through producing sour screening experiment, successfully screen the bacterial strain C11 that lactic acid producing performance is high, Fig. 1 is the light micrograph of C11.
The qualification of 2.2 lactic acid producing bacilluss
2.2.1 morphological feature
Bacterial strain C11 is well-grown on YPD substratum, and 48h forms the circular colonies of diameter 2 ~ 3mm size, white and glossy, surface wettability, smooth, neat in edge.
Oil sem observation, bacterial strain C11 thalli morphology is shaft-like, single, paired or catenation, and brood cell holds life, as shown in Figure 1.
2.2.2 physiological and biochemical property, specific features value is in table 15.
Table 11-1:
Project Gramstaining Mobility 37 oC grows Anaerobic growth Oxydase Catalase
C11 ＋ ＋ ＋ ＋ ＋ ＋
Table 11-2:
Project Glucose oxidase ferments Methyl red V-P measures Starch Hydrolysis Indoles produces Gelatine liquefication
C11 Fermentation ＋ ＋ ＋ － －
Description about Bacillus on above-mentioned qualification result and " uncle Jie Shi Bacteria Identification handbook " contrasted, C11 belongs to Bacillus (Bacillusspp.), subspecies of bacillus coagulans (B.coagulans).
2.2.316SrDNA the pcr amplification of gene and sequential analysis
With P0 and PC3 for primer, bacterial strain C11 STb gene is that template carries out pcr amplification.Optimize the extension rate of buffer system, magnesium ion concentration and template DNA, the specific band of about 700bp can be amplified.
The band of the 700bp of cleavage specificity from sepharose, test kit reclaims purified pcr product, carries out the mensuration of DNA sequence dna.Measured sequence is carried out similarity analysis with the 16SrDNA sequence in Genbank compare.The homology of the 16SrDNA sequence of bacterial strain C11 and Bacilluscoagulans reaches 99%, in the sequence of total length 746bp, only has the difference of 1bp.
The result of comprehensive Physiology and biochemistry qualification and 16SrRNA order-checking, can think that bacterial strain C11 lactic acid producing performance is high and ability of disposing of sewage in severe environment is the strongest, the synergy between the maximum performance functional microbial of energy.
Above content is only preferred embodiment of the present invention, and for those of ordinary skill in the art, according to thought of the present invention, all will change in specific embodiments and applications, this description should not be construed as limitation of the present invention.

Claims (8)

1. strengthen a method for the reconstruction of black-odor river Tiny ecosystem and balance and water correction, it is characterized in that, comprise the steps:
(1) complex microorganism is prepared, be that 6 ~ 25:5 ~ 10:15 ~ 35 mix by weight by complex micro organism fungicide A, complex micro organism fungicide B and complex micro organism fungicide C, obtained complex microorganism, described complex micro organism fungicide A comprises Rhodopseudomonas spheroides, described complex micro organism fungicide B comprises bacillus JY-LZ, and described complex micro organism fungicide C comprises the organic mineralized bacterium of low temperature;
(2) Biological Pretreatment is carried out to target river course; Gushing planting aquatic plants with main each of bordering on of gushing, and throwing in described complex microorganism 30 ~ 320ppm/ days, described waterplant and complex microorganism form biological reinforced band;
(3) the biological internal recycle respiratory system gushed in river and cometabolism mechanism is built; Gush interior layout molecule bubble oxygen to transmit and surperficial oxygenation combination aeration system in target river, to promote space flourish and the morphology control of complex microorganism;
(4) advanced treatment of polluted bed mud and reduction, balance bed mud structure, sets up river and gushes bottom original position step circular treatment microorganism bed; Gush interior input bio-based, biological brick and high molecule microcapsule to target river, and drop into described complex microorganism 30 ~ 320ppm/ days, make the carbon-nitrogen ratio C/N of adjustment aim river water burst body be 4:1 ~ 8:1, N/P ratio N/P is 7:1 ~ 25:1, and biological carbon is 2.3 ~ 6.2PPM; To carry out bio-oxidation to polluted bed mud, row oxygenation process is poured into target river simultaneously;
(5) ecological management of water body and deep purifying, rebuilds river and gushes microecosystem and balance; Planting aquatic plants builds biological floating island, continues to throw in described complex microorganism 30 ~ 320ppm/ days, and make the carbon-nitrogen ratio C/N of adjustment aim river water burst body be 4:1 ~ 8:1, N/P ratio N/P is 7:1 ~ 25:1;
(6) water ecology reparation and multi-functional regulation and control, put hydrocoles in gushing to target river, and continue to throw in described complex microorganism 30 ~ 320ppm/ days, and make the carbon-nitrogen ratio C/N of adjustment aim river water burst body be 4:1 ~ 8:1, N/P ratio N/P is 7:1 ~ 25:1.
2. method according to claim 1, is characterized in that: described complex micro organism fungicide A comprises following bacterial classification: spherical Rhodopseudomonas: Rhodopseudomonas palustris: capsula Rhodopseudomonas: withered grass bud robe bacillus: bacillus cereus: Bacillus licheniformis: denitrifying bacterium: milk-acid bacteria: yeast: the weight ratio of colloid bacillus cereus is 2.0 ~ 8.0:1.5 ~ 5.8:1.0 ~ 5.0:2.0 ~ 6.0:1.2 ~ 4.6:1.0 ~ 4.0:2.0 ~ 6.5:1.0 ~ 3.5:0.5 ~ 3.0:0.3 ~ 3.2.
3. method according to claim 1, is characterized in that: described complex micro organism fungicide B comprises following bacterial classification: bacillus JY-LZ: spherical Rhodopseudomonas: bacillus coagulans: bacillus laterosporus: Bacillus licheniformis: milk-acid bacteria: yeast: actinomycetes: the weight ratio of azotobacter chroococcum is 1.6 ~ 5.6:0.5 ~ 2.5:1.3 ~ 6.5:1.0 ~ 4.0:1.0 ~ 3.0:0.5 ~ 1.2:0.5 ~ 2.5:0.2 ~ 1.5:0.4 ~ 2.8.
4. method according to claim 1, is characterized in that: described complex micro organism fungicide C comprises following bacterial classification: the organic mineralized bacterium of low temperature: Rhodopseudomonas palustris: withered grass bud robe bacillus: colloid bacillus cereus: bacillus laterosporus: bacillus pumilus: nitrobacteria: denitrifying bacterium: milk-acid bacteria: yeast: the weight ratio of azotobacter chroococcum is 2.0 ~ 10:1.0 ~ 5.0:1.5 ~ 7.5:1.2 ~ 6.4:1.0 ~ 3.5:1.0 ~ 3.0:0.6 ~ 4.0:1.5 ~ 5.8:0.5 ~ 4.5:0.5 ~ 2.5:0.4 ~ 3.5.
5. method according to claim 1, is characterized in that: screen, cultivate described Rhodopseudomonas spheroides, bacillus JY-LZ, sample needed for the organic mineralized bacterium of low temperature respectively from target canal reactor and/or bed mud.
6. method according to claim 1, is characterized in that: in step (2), and the fraction of coverage of described waterplant is 1 ~ 5%;
Preferably, described waterplant is one or more in reed, Caulis Miscanthis floriduli, Scirpus tabernaemontani, aquatic Canna generalis Bailey, cyperus alternifolius;
Preferably, the planting density of described waterplant is 15 ~ 30 strains/m 2;
Preferably, in step (4), described bio-based is containing, for example the component of lower massfraction: medical stone 20% ~ 35%, high molecule microcapsule 35% ~ 75%;
Preferably, the particle diameter of described medical stone is 20 ~ 40mm;
Preferably, the particle diameter of described high molecule microcapsule is 500 ~ 3000 μm;
Preferably, described biological brick is containing, for example the component of lower massfraction: medical stone 10% ~ 35%, fine sand 10% ~ 25%, Water Quenching Slag 10% ~ 20%, pebbles 15% ~ 30%, zeolite 15% ~ 40%;
Preferably, the particle diameter of described pebbles is 30 ~ 60mm;
Preferably, the particle diameter of described medical stone, fine sand, Water Quenching Slag, zeolite is respectively 20 ~ 40mm;
Preferably, in step (5), the preparation process of described biological floating island is: mixed with biological floating island matrix by described complex microorganism, the weight ratio of described complex microorganism and biological floating island matrix is 1:10 ~ 20, be filled in a body, planting aquatic plants, obtain biological floating island;
Preferably, described biological floating island matrix is containing, for example the component of lower massfraction: medical stone 20% ~ 55%, fine sand 15% ~ 25%, Water Quenching Slag 15% ~ 30%, zeolite 25% ~ 65%;
Preferably, the particle diameter of described medical stone, fine sand, Water Quenching Slag, zeolite is respectively 20 ~ 40mm;
Preferably, described waterplant is one or more in reed, Caulis Miscanthis floriduli, Scirpus tabernaemontani, aquatic Canna generalis Bailey, cyperus alternifolius;
Preferably, the planting density of described waterplant is 15 ~ 20 strains/m 2;
Preferably, each biological floating island area is 8 ~ 15m 2;
Preferably, the spacing between adjacent two biological floating islands is 20 meters;
Preferably, in step (6), described hydrocoles comprises silver carp, variegated carp, spiral shell and zooplankton;
Preferably, described silver carp: variegated carp: spiral shell: the weight ratio of zooplankton is 3:1:0.5:0.2.
7. method according to claim 1, it is characterized in that: in step (1), screen, cultivate the Rhodopseudomonas spheroides of described complex micro organism fungicide A, the bacillus JY-LZ of complex micro organism fungicide B, the sewage that gushes from the smelly Heihe of target respectively of the sample needed for the organic mineralized bacterium of low temperature of complex micro organism fungicide C and/or bed mud.
8. method according to claim 1, is characterized in that, in step (1), in described complex micro organism fungicide A, the cultivation of spherical Rhodopseudomonas and screening comprise the steps:
(1a) enrichment medium and isolation medium is prepared respectively;
(1b) enrichment culture; The black smelly bed mud 1 ~ 2g in river course getting collection is inoculated in enrichment medium, and liquid level injects sterilising liq paraffin, starvation, forms anaerobic environment, is statically placed in 28 ~ 30 DEG C of cultivations in illumination box, observes at any time; When there is blush grower in culture, getting 0.5 ~ 1.3ml enrichment culture thing from blush grower, being inoculated in another enrichment medium and cultivating; Repeat enrichment culture 2 ~ 3 times;
(1c) be separated through plate streaking successively, shake tube method separation, screen and after slant culture, obtain spherical Rhodopseudomonas;
Preferably, in step (1a), described enrichment medium is containing, for example lower following component: NH 4cl0.8g ~ 1.8g, K 2hPO 40.2 ~ 0.7g, MgSO 40.1 ~ 0.3g, NaCl1.5 ~ 2.5g, yeast extract paste 0.1 ~ 0.3g, NaHCO 31 ~ 5g, dehydrated alcohol 1.5 ~ 2.0ml, liquid microelement 1.0mL;
Preferably, described liquid microelement is containing, for example lower component: H 3bO 30.006 ~ 0.012mg, NaMoO 40.002 ~ 0.008mg, MnSO 40.0005 ~ 0.002mg, ZnSO 40.002 ~ 0.006mg, distilled water constant volume;
Preferably, in step (1a), described isolation medium is containing, for example lower composition: NH 4cl0.8g ~ 1.5g, K 2hPO 40.2 ~ 0.7g, MgSO 40.1 ~ 0.5g, NaCl1.5 ~ 3.0g, yeast extract paste 0.1 ~ 0.3g, NaHCO 31 ~ 3g, dehydrated alcohol 1.5 ~ 2.0ml, agar powder 1.5 ~ 2.0g, Na 2s9H 2o1 ~ 2g.
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CN109293131A (en) * 2018-09-18 2019-02-01 广州微问环保科技有限公司 A kind of method of original position self-purification black and odorous water
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CN109607988A (en) * 2019-02-22 2019-04-12 合肥市东方美捷分子材料技术有限公司 A kind of ecology pit black and odorous water processing method
CN109678257A (en) * 2019-03-01 2019-04-26 北京高能时代环境技术股份有限公司 A method of being suitable for the functional flora construction method and application flora improvement black and odorous water of black and odorous water improvement
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