CN106745804A - A kind of microorganism High-speed clarification agent for black and odorous water and preparation method thereof - Google Patents
A kind of microorganism High-speed clarification agent for black and odorous water and preparation method thereof Download PDFInfo
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- CN106745804A CN106745804A CN201611168091.5A CN201611168091A CN106745804A CN 106745804 A CN106745804 A CN 106745804A CN 201611168091 A CN201611168091 A CN 201611168091A CN 106745804 A CN106745804 A CN 106745804A
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- water
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- 244000005700 microbiome Species 0.000 title claims abstract description 131
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 100
- 238000005352 clarification Methods 0.000 title claims abstract description 57
- 239000003795 chemical substances by application Substances 0.000 title claims abstract description 51
- 238000002360 preparation method Methods 0.000 title claims description 24
- 239000007788 liquid Substances 0.000 claims abstract description 123
- 241000894006 Bacteria Species 0.000 claims abstract description 22
- 229910052782 aluminium Inorganic materials 0.000 claims abstract description 19
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminum Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 claims abstract description 19
- FAPWRFPIFSIZLT-UHFFFAOYSA-M sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 32
- VTYYLEPIZMXCLO-UHFFFAOYSA-L calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 16
- 239000006025 fining agent Substances 0.000 claims description 16
- CSNNHWWHGAXBCP-UHFFFAOYSA-L mgso4 Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 16
- 239000011780 sodium chloride Substances 0.000 claims description 16
- 239000001963 growth media Substances 0.000 claims description 15
- 238000002955 isolation Methods 0.000 claims description 15
- 238000000746 purification Methods 0.000 claims description 15
- 239000002609 media Substances 0.000 claims description 14
- 229940041514 Candida albicans extract Drugs 0.000 claims description 12
- 239000001888 Peptone Substances 0.000 claims description 12
- 108010080698 Peptones Proteins 0.000 claims description 12
- 235000019319 peptone Nutrition 0.000 claims description 12
- 239000012138 yeast extract Substances 0.000 claims description 12
- 238000000926 separation method Methods 0.000 claims description 11
- BFNBIHQBYMNNAN-UHFFFAOYSA-N Ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 8
- 229960003563 Calcium Carbonate Drugs 0.000 claims description 8
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L MgCl2 Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 claims description 8
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 8
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 8
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 8
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 8
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 8
- NWONKYPBYAMBJT-UHFFFAOYSA-L Zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 7
- 239000004411 aluminium Substances 0.000 claims description 7
- SURQXAFEQWPFPV-UHFFFAOYSA-L iron(2+) sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Fe+2].[O-]S([O-])(=O)=O SURQXAFEQWPFPV-UHFFFAOYSA-L 0.000 claims description 7
- CNFDGXZLMLFIJV-UHFFFAOYSA-L manganese(II) chloride tetrahydrate Chemical compound O.O.O.O.[Cl-].[Cl-].[Mn+2] CNFDGXZLMLFIJV-UHFFFAOYSA-L 0.000 claims description 7
- 239000000203 mixture Substances 0.000 claims description 7
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 7
- 239000011686 zinc sulphate Substances 0.000 claims description 7
- 235000009529 zinc sulphate Nutrition 0.000 claims description 7
- ZPWVASYFFYYZEW-UHFFFAOYSA-L Dipotassium phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 6
- NBIIXXVUZAFLBC-UHFFFAOYSA-N phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims description 6
- FDEIWTXVNPKYDL-UHFFFAOYSA-N sodium molybdate dihydrate Chemical compound O.O.[Na+].[Na+].[O-][Mo]([O-])(=O)=O FDEIWTXVNPKYDL-UHFFFAOYSA-N 0.000 claims description 6
- 239000002068 microbial inoculum Substances 0.000 claims description 5
- 238000005660 chlorination reaction Methods 0.000 claims description 4
- 229910001629 magnesium chloride Inorganic materials 0.000 claims description 4
- KEAYESYHFKHZAL-UHFFFAOYSA-N sodium Chemical compound [Na] KEAYESYHFKHZAL-UHFFFAOYSA-N 0.000 claims description 4
- 239000011734 sodium Substances 0.000 claims description 4
- 229910052708 sodium Inorganic materials 0.000 claims description 4
- GNSKLFRGEWLPPA-UHFFFAOYSA-M Monopotassium phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 3
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims description 3
- UFHFLCQGNIYNRP-UHFFFAOYSA-N hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 claims description 3
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 3
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 3
- BAUYGSIQEAFULO-UHFFFAOYSA-L Iron(II) sulfate Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims 1
- TVXXNOYZHKPKGW-UHFFFAOYSA-N Sodium molybdate Chemical compound [Na+].[Na+].[O-][Mo]([O-])(=O)=O TVXXNOYZHKPKGW-UHFFFAOYSA-N 0.000 claims 1
- 239000011790 ferrous sulphate Substances 0.000 claims 1
- 235000003891 ferrous sulphate Nutrition 0.000 claims 1
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims 1
- 239000011591 potassium Substances 0.000 claims 1
- 229910052700 potassium Inorganic materials 0.000 claims 1
- 239000011684 sodium molybdate Substances 0.000 claims 1
- 235000015393 sodium molybdate Nutrition 0.000 claims 1
- 239000002994 raw material Substances 0.000 abstract description 2
- 210000000697 sensory organs Anatomy 0.000 abstract description 2
- 239000010865 sewage Substances 0.000 abstract description 2
- 206010042602 Supraventricular extrasystoles Diseases 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 229910052739 hydrogen Inorganic materials 0.000 description 2
- 239000001257 hydrogen Substances 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 241000124033 Salix Species 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 230000002906 microbiologic Effects 0.000 description 1
- 238000011022 operating instruction Methods 0.000 description 1
- 238000000197 pyrolysis Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
Abstract
The invention discloses a kind of microorganism High-speed clarification agent administered for black and odorous water, belong to the biological treatment field of sewage.The High-speed clarification agent includes：The weight portion of A liquid 0.5~10, the weight portion of B liquid 1~3 and the weight portion of C liquid 0.1~1；Wherein, A liquid is polyaluminium aluminum water solution；B liquid is indigenous microorganism bacterium solution；C liquid is liquid microelement.The High-speed clarification agent is raw material using river course bed mud in itself, separates the strain of degraded black and odorous water, as B liquid.A liquid and C liquid are added, microorganism High-speed clarification agent is configured as.Black biological High-speed clarification agent is added in black and odorous water, can clarify in 4h black and odorous water, reach comfortable sense organ degree.
Description
Technical field
The invention belongs to the microbiological treatment field of sewage, more particularly to a kind of micro- life administered for river black and odorous water
Thing High-speed clarification agent and preparation method thereof.
Background technology
With《City black and odorous water operating instructions》Issue, the improvement of black and odorous water has certain standard.Typically may be used
To point source, face source and endogenous to be administered using Physical, chemical method and bioanalysis.Bioanalysis is most passed through in all methods
The method of Ji.The shortcoming of bioanalysis is slow processing speed.For disadvantage mentioned above, using microorganism High-speed clarification agent, can in 4h
Make black and odorous water High-speed clarification, reach comfortable transparency.The patent of invention of the patent No. 201010610486.2, the patent is provided
A kind of microorganism is to black-odor riverway treatment and the method for restoration of the ecosystem in situ.It is using matrix, water plant and complex microorganism
Microbial inoculum is processed to be made the mode of biological floating island.Belong to the endogenous improvement of black and odorous water.Complex operation, it is relatively costly.
The patent of invention of the patent No. 201510795860.3, the patent provides a kind of complex microorganism administered for river black and odorous water
Preparation and preparation method, using and methods for using them.The patent needs high-temperature service to prepare willow block, and the temperature of pyrolysis needs height
Up to 180~240 DEG C.The energy is not only wasted in manufacturing process, and has safety issue.Prepare time of microorganism formulation compared with
It is long, and the onset time is also more long in being put into black and odorous water, it is impossible to it is rapidly achieved Expected Results.
The content of the invention
It is fast it is an object of the invention to provide a kind of microorganism for black and odorous water based on the problems of prior art
Fast fining agent and preparation method thereof, simple to operate, cost is relatively low, enables black and odorous water High-speed clarification in input black and odorous water, soon
Speed produces a desired effect.
The purpose of the present invention is achieved through the following technical solutions：
The embodiment of the present invention provides a kind of microorganism High-speed clarification agent for black and odorous water, and the High-speed clarification agent includes：A
The weight portion of liquid 0.5~10, the weight portion of B liquid 1~3 and the weight portion of C liquid 0.1~1；
Wherein, A liquid is polyaluminium aluminum water solution；B liquid is indigenous microorganism bacterium solution；C liquid is liquid microelement.
The embodiment of the present invention provides a kind of preparation method of microorganism High-speed clarification agent for black and odorous water, for preparing
High-speed clarification agent of the present invention, comprises the following steps：
The liquid microelement of the polyaluminium aluminum water solution of A liquid, the indigenous microorganism bacterium solution of B liquid and C liquid is prepared respectively；
A liquid, B liquid and C liquid are taken by the weight portion of A liquid 0.5~10, the weight portion of B liquid 1~3 and the weight portion of C liquid 0.1~1, is prepared
When, during C liquid first added into B liquid, stir and evenly mix, A liquid is added after 30min, stand the micro- life being obtained after 1h for black and odorous water
Thing High-speed clarification agent.
As seen from the above technical solution provided by the invention, it is provided in an embodiment of the present invention for the micro- of black and odorous water
Biological High-speed clarification agent, by the polyaluminium aluminum water solution of the A liquid of reasonable volume, the indigenous microorganism bacterium solution of B liquid and C liquid
Liquid microelement coordinates, and black and odorous water High-speed clarification is enable in input black and odorous water, can be rapidly achieved expected clarifying effect, and this is clear
Clear agent preparation manipulation is simple, and cost is relatively low.
Specific embodiment
With reference to the content in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Ground description, it is clear that described embodiment is only a part of embodiment of the invention, rather than whole embodiments.Based on this
Inventive embodiment, the every other implementation that those of ordinary skill in the art are obtained under the premise of creative work is not made
Example, belongs to protection scope of the present invention.
The embodiment of the present invention provides a kind of microorganism High-speed clarification agent for black and odorous water, for river black and odorous water
High-speed clarification, the High-speed clarification agent includes：The weight portion of A liquid 0.5~10, the weight portion of B liquid 1~3 and the weight portion of C liquid 0.1~1；
Wherein, A liquid is polyaluminium aluminum water solution；B liquid is indigenous microorganism bacterium solution；C liquid is liquid microelement.The clarification
When agent is prepared, during C liquid first added into B liquid, stir and evenly mix, A liquid is added after 30min, be obtained for black and odorous water after standing 1h
Microorganism High-speed clarification agent.When using, the dosage of the fining agent is 0.01~0.1m3The microorganism High-speed clarification agent/m2River
Road.
In above-mentioned fining agent, the polyaluminium aluminum water solution of A liquid by the aluminium polychloride of 1~10 weight portion and 500~
The water composition of 1000 weight portions.
In above-mentioned fining agent, the liquid microelement of C liquid by：The weight portion of white vitriol 0.2~0.4, tetrahydrate manganese chloride 0.1
~1 weight portion, the weight portion of Sodium Molybdate Dihydrate 0.2~0.3 and the weight portion of water 1000 are constituted.
In above-mentioned fining agent, the indigenous microorganism bacterium solution of B liquid is obtained in the following ways：Choose the bottom in river course to be administered
Mud, accesses in indigenous microorganism enriched medium, and the rotating speed of 120~180r/min carries out shaking table culture under the conditions of 28~38 DEG C
1~3 day, indigenous microorganism pregnant solution is obtained；
The indigenous microorganism pregnant solution by obtained in is accessed in indigenous microorganism isolation and purification culture base, and 28~38
The rotating speed of 120~180r/min carries out shaking table culture 3~10 days under the conditions of DEG C, and indigenous microorganism refined solution is obtained；
By obtained indigenous microorganism refined solution access indigenous microorganism separation screening culture medium in, in 28~38 DEG C of conditions
The rotating speed of lower 120~180r/min carries out microbial inoculum as indigenous microorganism bacterium solution obtained in shaking table culture 1~3 day.
In above-mentioned fining agent, indigenous microorganism enriched medium includes：The weight portion of peptone 1~30, the weight of yeast extract 1~10
Amount part, the weight portion of sodium chloride 5~20 and the weight portion of water 1000；The pH value of the indigenous microorganism enriched medium is 6~8.
In above-mentioned fining agent, indigenous microorganism isolation and purification culture base includes：The weight portion of peptone 1~30, yeast extract 1~
10 weight portions, the weight portion of sodium chloride 5~20, the weight portion of ammonium sulfate 0.5~5, the weight portion of epsom salt 0.1~0.3, phosphoric acid hydrogen
The weight portion of dipotassium 0.2~0.6, the weight portion of ferrous sulfate heptahydrate 0.05~0.4, the weight portion of sodium chloride 0.1~4, calcium carbonate 1~5
Weight portion and the weight portion of water 1000, the pH value of the indigenous microorganism isolation and purification culture base is 6~8.
In above-mentioned fining agent, indigenous microorganism separation screening culture medium includes：The weight portion of peptone 1~30, yeast extract 1~
10 weight portions, the weight portion of sodium chloride 5~20, the weight portion of ammonium sulfate 0.1~3, the weight portion of epsom salt 0.1~0.3, phosphoric acid hydrogen
The weight portion of dipotassium 0.1~0.5, the weight portion of potassium dihydrogen phosphate 0.01~0.5, the weight portion of ferrous sulfate heptahydrate 0.05~0.1, chlorination
The weight portion of sodium 0.1~1, the weight portion of magnesium chloride 0.1~0.5, the weight portion of calcium carbonate 1~3 and the weight portion of water 1000, indigenous micro- life
The pH value of thing separation screening culture medium is 6~8.
The embodiment of the present invention also provides a kind of preparation method of the microorganism High-speed clarification agent for black and odorous water, for making
Standby above-mentioned High-speed clarification agent, comprises the following steps：
The liquid microelement of the polyaluminium aluminum water solution of A liquid, the indigenous microorganism bacterium solution of B liquid and C liquid is prepared respectively；
A liquid, B liquid and C liquid are taken by the weight portion of A liquid 0.5~10, the weight portion of B liquid 1~3 and the weight portion of C liquid 0.1~1, is prepared
When, during C liquid first added into B liquid, stir and evenly mix, A liquid is added after 30min, stand the micro- life being obtained after 1h for black and odorous water
Thing High-speed clarification agent.
In above-mentioned preparation method, the polyaluminium aluminum water solution of A liquid by the aluminium polychloride of 1~10 weight portion and 500~
The water composition of 1000 weight portions；
The liquid microelement of the C liquid by：The weight portion of white vitriol 0.2~0.4, the weight of tetrahydrate manganese chloride 0.1~1
Part, the weight portion of Sodium Molybdate Dihydrate 0.2~0.3 and the weight portion of water 1000 are constituted；
The indigenous microorganism bacterium solution of the B liquid is obtained in the following ways：The bed mud in river course to be administered is chosen, original inhabitants are accessed
In microorganism enriched medium, the rotating speed of 120~180r/min carries out shaking table culture 1~3 day under the conditions of 28~38 DEG C, is obtained
Indigenous microorganism pregnant solution；The indigenous microorganism pregnant solution by obtained in is accessed in indigenous microorganism isolation and purification culture base,
And the rotating speed of 120~180r/min carries out shaking table culture 3~10 days under the conditions of 28~38 DEG C, indigenous microorganism purifying is obtained
Liquid；By obtained indigenous microorganism refined solution access indigenous microorganism separation screening culture medium in, 120 under the conditions of 28~38 DEG C
The rotating speed of~180r/min carries out microbial inoculum as indigenous microorganism bacterium solution obtained in shaking table culture 1~3 day；Wherein,
The indigenous microorganism enriched medium includes：The weight portion of peptone 1~30, the weight portion of yeast extract 1~10, chlorination
The weight portion of sodium 5~20 and the weight portion of water 1000；The pH value of the indigenous microorganism enriched medium is 6~8；
The indigenous microorganism isolation and purification culture base includes：The weight portion of peptone 1~30, the weight portion of yeast extract 1~10,
The weight portion of sodium chloride 5~20, the weight portion of ammonium sulfate 0.5~5, the weight portion of epsom salt 0.1~0.3, dipotassium hydrogen phosphate 0.2~
0.6 weight portion, the weight portion of ferrous sulfate heptahydrate 0.05~0.4, the weight portion of sodium chloride 0.1~4, the weight portion of calcium carbonate 1~5 and water
1000 weight portions, the pH value of the indigenous microorganism isolation and purification culture base is 6~8；
The indigenous microorganism separation screening culture medium includes：The weight portion of peptone 1~30, the weight portion of yeast extract 1~10,
The weight portion of sodium chloride 5~20, the weight portion of ammonium sulfate 0.1~3, the weight portion of epsom salt 0.1~0.3, dipotassium hydrogen phosphate 0.1~
0.5 weight portion, the weight portion of potassium dihydrogen phosphate 0.01~0.5, the weight portion of ferrous sulfate heptahydrate 0.05~0.1, sodium chloride 0.1~1
Weight portion, the weight portion of magnesium chloride 0.1~0.5, the weight portion of calcium carbonate 1~3 and the weight portion of water 1000, the indigenous microorganism bolter
The pH value for selecting culture medium is 6~8.
The above-mentioned dosage for preparing fining agent is 0.01~0.1m3The microorganism High-speed clarification agent/m2River course.
Fining agent of the invention is described in further detail below in conjunction with specific embodiment.
Embodiment one
The present embodiment provides a kind of microorganism High-speed clarification agent for black and odorous water, and the fining agent preparation method is as follows：
According to the weight portion of A liquid 10, the weight portion of B liquid 3, the weight of the weight portion of C liquid 1 is than configuration black and odorous water High-speed clarification liquid.
Wherein, by 10 weight portion PAC (aluminium polychloride), the weight portion of water 1000 is obtained A liquid.
B liquid is made up of indigenous microorganism bacterium solution.Preparation method：The bed mud in river course to be administered is chosen, indigenous microorganism is accessed
In enriched medium, the rotating speed of 180r/min carries out shaking table culture 3 days under the conditions of 38 DEG C, and indigenous microorganism pregnant solution is obtained.
By indigenous microorganism pregnant solution access indigenous microorganism isolation and purification culture base in, and under the conditions of 35 DEG C 160r/min rotating speed
Carry out shaking table culture 10 days, indigenous microorganism refined solution is obtained.Indigenous microorganism refined solution is accessed into indigenous microorganism bolter
Select in culture medium, the rotating speed of 180r/min carries out shaking table culture 3 days under the conditions of 28 DEG C, indigenous microorganism bacterium solution is obtained.
C liquid is made up of liquid microelement.Preparation method：The weight portion of white vitriol 0.2, the weight portion of tetrahydrate manganese chloride 0.1,
The weight portion of Sodium Molybdate Dihydrate 0.2, the weight portion of water 1000.
Microorganism High-speed clarification agent is added to Beijing river black and odorous water section, dosage is 0.07m3Microorganism High-speed clarification
Agent/m2River course.Water quality index see the table below before adding and after adding 4h
Embodiment two
The present embodiment provides a kind of microorganism High-speed clarification agent for black and odorous water, and the fining agent preparation method is as follows：
According to the weight portion of A liquid 5, the weight portion of B liquid 1, the weight of the weight portion of C liquid 0.5 is than configuration black and odorous water High-speed clarification liquid.
Wherein, by 10 weight portion PAC (aluminium polychloride), the weight portion of water 1000 is obtained A liquid.
B liquid is made up of indigenous microorganism bacterium solution.Preparation method：The bed mud in river course to be administered is chosen, indigenous microorganism is accessed
In enriched medium, the rotating speed of 180r/min carries out shaking table culture 3 days under the conditions of 38 DEG C, and indigenous microorganism pregnant solution is obtained.
By indigenous microorganism pregnant solution access indigenous microorganism isolation and purification culture base in, and under the conditions of 35 DEG C 160r/min rotating speed
Carry out shaking table culture 10 days, indigenous microorganism refined solution is obtained.Indigenous microorganism refined solution is accessed into indigenous microorganism bolter
Select in culture medium, the rotating speed of 180r/min carries out shaking table culture 3 days under the conditions of 28 DEG C, indigenous microorganism bacterium solution is obtained.
C liquid is made up of liquid microelement.Preparation method：The weight portion of white vitriol 0.2, the weight portion of tetrahydrate manganese chloride 0.1,
The weight portion of Sodium Molybdate Dihydrate 0.2, the weight portion of water 1000.
Microorganism High-speed clarification agent is added to Beijing river black and odorous water section, dosage is 0.04m3Microorganism High-speed clarification
Agent/m2River course.Water quality index see the table below before adding and after adding 4h
Microorganism High-speed clarification agent is added to Jilin Changchun river black and odorous water section, before adding and rear water quality index is added and is seen
Following table
Embodiment three
The present embodiment provides a kind of microorganism High-speed clarification agent for black and odorous water, and the fining agent preparation method is as follows：
According to the weight portion of A liquid 7, the weight portion of B liquid 1, the weight of the weight portion of C liquid 0.5 is than configuration black and odorous water High-speed clarification liquid.
Wherein, A liquid is obtained by 10 weight portion PAC (aluminium polychloride) and the weight portion of water 1000.
B liquid is indigenous microorganism bacterium solution, and its preparation method is：The bed mud in river course to be administered is chosen, indigenous microorganism is accessed
In enriched medium, the rotating speed of 180r/min carries out shaking table culture 3 days under the conditions of 38 DEG C, and indigenous microorganism pregnant solution is obtained.
By indigenous microorganism pregnant solution access indigenous microorganism isolation and purification culture base in, and under the conditions of 35 DEG C 160r/min rotating speed
Carry out shaking table culture 10 days, indigenous microorganism refined solution is obtained.Indigenous microorganism refined solution is accessed into indigenous microorganism bolter
Select in culture medium, the rotating speed of 180r/min carries out shaking table culture 3 days under the conditions of 28 DEG C, indigenous microorganism bacterium solution is obtained.
C liquid is liquid microelement, and its preparation method is：The weight portion of white vitriol 0.2, the weight portion of tetrahydrate manganese chloride 0.1,
The weight portion of Sodium Molybdate Dihydrate 0.2, the weight portion of water 1000.
Microorganism High-speed clarification agent is added to Beijing river black and odorous water section, dosage is 0.04m3Microorganism High-speed clarification
Agent/m2River course.Water quality index see the table below before adding and after adding 4h
Microorganism High-speed clarification agent is added to Hebei section black and odorous water, before adding and rear water quality index is added and be see the table below
By the various embodiments described above as can be seen that fining agent of the invention is raw material using river course bed mud in itself, point
From the strain of degraded black and odorous water is filtered out as B liquid, A liquid and C liquid are added, microorganism High-speed clarification agent is configured as, black
Black biological High-speed clarification agent is added in smelly water body, can clarify in 4h black and odorous water, reach comfortable sense organ degree, this is quick clear
Clear black and odorous water, with preparation and simple to operate, the good advantage of clarifying effect.
The above, the only present invention preferably specific embodiment, but protection scope of the present invention is not limited thereto,
Any one skilled in the art in the technical scope of present disclosure, the change or replacement that can be readily occurred in,
Should all be included within the scope of the present invention.Therefore, protection scope of the present invention should be with the protection model of claims
Enclose and be defined.
Claims (10)
1. a kind of microorganism High-speed clarification agent for black and odorous water, it is characterised in that the High-speed clarification agent includes：A liquid 0.5~
10 weight portions, the weight portion of B liquid 1~3 and the weight portion of C liquid 0.1~1；
Wherein, A liquid is polyaluminium aluminum water solution；B liquid is indigenous microorganism bacterium solution；C liquid is liquid microelement.
2. a kind of microorganism High-speed clarification agent for black and odorous water according to claim 1, it is characterised in that the A
The polyaluminium aluminum water solution of liquid is made up of the aluminium polychloride of 1~10 weight portion with the water of 500~1000 weight portions.
3. a kind of microorganism High-speed clarification agent for black and odorous water as claimed in claim 1, it is characterised in that the C liquid
Liquid microelement by：The weight portion of white vitriol 0.2~0.4, the weight portion of tetrahydrate manganese chloride 0.1~1, Sodium Molybdate Dihydrate 0.2
~0.3 weight portion and the weight portion of water 1000 are constituted.
4. a kind of microorganism High-speed clarification agent for black and odorous water according to any one of claims 1 to 3, its feature exists
In the indigenous microorganism bacterium solution of the B liquid is obtained in the following ways：The bed mud in river course to be administered is chosen, indigenous micro- life is accessed
In thing enriched medium, the rotating speed of 120~180r/min carries out shaking table culture 1~3 day under the conditions of 28~38 DEG C, and original inhabitants are obtained
Enrichment by Microorganisms liquid collecting；
The indigenous microorganism pregnant solution by obtained in is accessed in indigenous microorganism isolation and purification culture base, and in 28~38 DEG C of bars
The rotating speed of 120~180r/min carries out shaking table culture 3~10 days under part, and indigenous microorganism refined solution is obtained；
By obtained indigenous microorganism refined solution access indigenous microorganism separation screening culture medium in, under the conditions of 28~38 DEG C
The rotating speed of 120~180r/min carries out microbial inoculum as indigenous microorganism bacterium solution obtained in shaking table culture 1~3 day.
5. a kind of microorganism High-speed clarification agent for black and odorous water as claimed in claim 3, the indigenous microorganism enrichment
Culture medium includes：The weight portion of peptone 1~30, the weight portion of yeast extract 1~10, the weight portion of sodium chloride 5~20 and the weight of water 1000
Part；The pH value of the indigenous microorganism enriched medium is 6~8.
6. a kind of microorganism High-speed clarification agent for black and odorous water as claimed in claim 3, it is characterised in that the original inhabitants
Microorganism isolation and purification culture base includes：The weight portion of peptone 1~30, the weight portion of yeast extract 1~10, the weight of sodium chloride 5~20
Part, the weight portion of ammonium sulfate 0.5~5, the weight portion of epsom salt 0.1~0.3, the weight portion of dipotassium hydrogen phosphate 0.2~0.6, seven water
The weight portion of ferrous sulfate 0.05~0.4, the weight portion of sodium chloride 0.1~4, the weight portion of calcium carbonate 1~5 and the weight portion of water 1000, should
The pH value of indigenous microorganism isolation and purification culture base is 6~8.
7. a kind of microorganism High-speed clarification agent for black and odorous water as claimed in claim 3, it is characterised in that the original inhabitants
Microorganism separation screening culture medium includes：The weight portion of peptone 1~30, the weight portion of yeast extract 1~10, the weight of sodium chloride 5~20
Part, the weight portion of ammonium sulfate 0.1~3, the weight portion of epsom salt 0.1~0.3, the weight portion of dipotassium hydrogen phosphate 0.1~0.5, phosphoric acid
The weight portion of potassium dihydrogen 0.01~0.5, the weight portion of ferrous sulfate heptahydrate 0.05~0.1, the weight portion of sodium chloride 0.1~1, magnesium chloride
0.1~0.5 weight portion, the weight portion of calcium carbonate 1~3 and the weight portion of water 1000, the pH of the indigenous microorganism separation screening culture medium
Be worth is 6~8.
8. the preparation method of a kind of microorganism High-speed clarification agent for black and odorous water, it is characterised in that will for preparing right
The High-speed clarification agent described in 1 to 7 any one is asked, is comprised the following steps：
The liquid microelement of the polyaluminium aluminum water solution of A liquid, the indigenous microorganism bacterium solution of B liquid and C liquid is prepared respectively；
A liquid, B liquid and C liquid are taken by the weight portion of A liquid 0.5~10, the weight portion of B liquid 1~3 and the weight portion of C liquid 0.1~1, during preparation, will
C liquid is first added in B liquid, is stirred and evenly mixed, and A liquid is added after 30min, is obtained after standing 1h quick for the microorganism of black and odorous water
Fining agent.
9. a kind of preparation method of microorganism High-speed clarification agent for black and odorous water according to claim 8, its feature
It is that the polyaluminium aluminum water solution of the A liquid is by the aluminium polychloride of 1~10 weight portion and the water of 500~1000 weight portions
Composition；
The liquid microelement of the C liquid by：The weight portion of white vitriol 0.2~0.4, the weight portion of tetrahydrate manganese chloride 0.1~1, two
The weight portion of water sodium molybdate 0.2~0.3 and the weight portion of water 1000 are constituted；
The indigenous microorganism bacterium solution of the B liquid is obtained in the following ways：The bed mud in river course to be administered is chosen, indigenous micro- life is accessed
In thing enriched medium, the rotating speed of 120~180r/min carries out shaking table culture 1~3 day under the conditions of 28~38 DEG C, and original inhabitants are obtained
Enrichment by Microorganisms liquid collecting；The indigenous microorganism pregnant solution by obtained in is accessed in indigenous microorganism isolation and purification culture base, and
The rotating speed of 120~180r/min carries out shaking table culture 3~10 days under the conditions of 28~38 DEG C, and indigenous microorganism refined solution is obtained；Will
Obtained indigenous microorganism refined solution is accessed in indigenous microorganism separation screening culture medium, 120 under the conditions of 28~38 DEG C~
The rotating speed of 180r/min carries out microbial inoculum as indigenous microorganism bacterium solution obtained in shaking table culture 1~3 day；Wherein,
The indigenous microorganism enriched medium includes：The weight portion of peptone 1~30, the weight portion of yeast extract 1~10, sodium chloride 5
~20 weight portions and the weight portion of water 1000；The pH value of the indigenous microorganism enriched medium is 6~8；
The indigenous microorganism isolation and purification culture base includes：The weight portion of peptone 1~30, the weight portion of yeast extract 1~10, chlorination
The weight portion of sodium 5~20, the weight portion of ammonium sulfate 0.5~5, the weight portion of epsom salt 0.1~0.3, dipotassium hydrogen phosphate 0.2~0.6
Weight portion, the weight portion of ferrous sulfate heptahydrate 0.05~0.4, the weight portion of sodium chloride 0.1~4, the weight portion of calcium carbonate 1~5 and water
1000 weight portions, the pH value of the indigenous microorganism isolation and purification culture base is 6~8；
The indigenous microorganism separation screening culture medium includes：The weight portion of peptone 1~30, the weight portion of yeast extract 1~10, chlorination
The weight portion of sodium 5~20, the weight portion of ammonium sulfate 0.1~3, the weight portion of epsom salt 0.1~0.3, dipotassium hydrogen phosphate 0.1~0.5
Weight portion, the weight portion of potassium dihydrogen phosphate 0.01~0.5, the weight portion of ferrous sulfate heptahydrate 0.05~0.1, the weight of sodium chloride 0.1~1
Part, the weight portion of magnesium chloride 0.1~0.5, the weight portion of calcium carbonate 1~3 and the weight portion of water 1000, indigenous microorganism separation screening training
The pH value for supporting base is 6~8.
10. the preparation method of a kind of microorganism High-speed clarification agent for black and odorous water according to claim 8 or claim 9, its
It is characterised by, the dosage of the microorganism High-speed clarification agent for black and odorous water is 0.01~0.1m3The microorganism is quick
Fining agent/m2River course.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108101231A (en) * | 2017-12-27 | 2018-06-01 | 桑德生态科技有限公司 | A kind of black and odorous water administers agent and method |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102050523A (en) * | 2010-12-29 | 2011-05-11 | 赵亚勋 | Method for treatment and in-situ ecological restoration of black and stink riverway by using microorganism |
| CN102583881A (en) * | 2012-02-13 | 2012-07-18 | 广东中大环保科技投资有限公司 | Method for treating water quality of black-odor river channel |
| CN103911332A (en) * | 2014-04-04 | 2014-07-09 | 范敏 | Method for culturing nitrogen and phosphorus removal composite bacteria of black and odorous water body |
| CN103922489A (en) * | 2014-05-09 | 2014-07-16 | 四川清和科技有限公司 | Microorganism composite flocculant coagulation bacterium application method |
| CN105174471A (en) * | 2015-09-16 | 2015-12-23 | 赵亚勋 | Reinforced black odorous river microecological reconstruction/balance and water quality improvement method |
| CN105417727A (en) * | 2015-12-21 | 2016-03-23 | 南京领先环保技术股份有限公司 | Method for deeply treating micro-polluted source water through in-situ enrichment, immobilization and acclimatization of indigenous microorganisms |
-
2016
- 2016-12-16 CN CN201611168091.5A patent/CN106745804B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102050523A (en) * | 2010-12-29 | 2011-05-11 | 赵亚勋 | Method for treatment and in-situ ecological restoration of black and stink riverway by using microorganism |
| CN102583881A (en) * | 2012-02-13 | 2012-07-18 | 广东中大环保科技投资有限公司 | Method for treating water quality of black-odor river channel |
| CN103911332A (en) * | 2014-04-04 | 2014-07-09 | 范敏 | Method for culturing nitrogen and phosphorus removal composite bacteria of black and odorous water body |
| CN103922489A (en) * | 2014-05-09 | 2014-07-16 | 四川清和科技有限公司 | Microorganism composite flocculant coagulation bacterium application method |
| CN105174471A (en) * | 2015-09-16 | 2015-12-23 | 赵亚勋 | Reinforced black odorous river microecological reconstruction/balance and water quality improvement method |
| CN105417727A (en) * | 2015-12-21 | 2016-03-23 | 南京领先环保技术股份有限公司 | Method for deeply treating micro-polluted source water through in-situ enrichment, immobilization and acclimatization of indigenous microorganisms |
Non-Patent Citations (1)
| Title |
|---|
| 王伟东等主编: "《生物制药工艺学》", 31 August 2015 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108101231A (en) * | 2017-12-27 | 2018-06-01 | 桑德生态科技有限公司 | A kind of black and odorous water administers agent and method |
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