CN106745804A - A kind of microorganism High-speed clarification agent for black and odorous water and preparation method thereof - Google Patents
A kind of microorganism High-speed clarification agent for black and odorous water and preparation method thereof Download PDFInfo
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- 244000005700 microbiome Species 0.000 title claims abstract description 90
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 85
- 238000002360 preparation method Methods 0.000 title claims description 23
- 239000003795 chemical substances by application Substances 0.000 title abstract description 7
- 238000005352 clarification Methods 0.000 title abstract 6
- 239000007788 liquid Substances 0.000 claims abstract description 91
- 239000000243 solution Substances 0.000 claims description 83
- 230000000813 microbial effect Effects 0.000 claims description 52
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 40
- 239000008395 clarifying agent Substances 0.000 claims description 40
- 239000001963 growth medium Substances 0.000 claims description 37
- 239000011780 sodium chloride Substances 0.000 claims description 20
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 claims description 18
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 16
- 238000000746 purification Methods 0.000 claims description 16
- 238000000926 separation method Methods 0.000 claims description 16
- 238000012216 screening Methods 0.000 claims description 15
- 239000011573 trace mineral Substances 0.000 claims description 14
- 235000013619 trace mineral Nutrition 0.000 claims description 14
- 239000001888 Peptone Substances 0.000 claims description 12
- 108010080698 Peptones Proteins 0.000 claims description 12
- 229940041514 candida albicans extract Drugs 0.000 claims description 12
- 235000019319 peptone Nutrition 0.000 claims description 12
- 239000012138 yeast extract Substances 0.000 claims description 12
- 239000007864 aqueous solution Substances 0.000 claims description 11
- 238000000034 method Methods 0.000 claims description 10
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 claims description 8
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 8
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 8
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 8
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 8
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 8
- SURQXAFEQWPFPV-UHFFFAOYSA-L iron(2+) sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Fe+2].[O-]S([O-])(=O)=O SURQXAFEQWPFPV-UHFFFAOYSA-L 0.000 claims description 8
- WRUGWIBCXHJTDG-UHFFFAOYSA-L magnesium sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Mg+2].[O-]S([O-])(=O)=O WRUGWIBCXHJTDG-UHFFFAOYSA-L 0.000 claims description 8
- 229940061634 magnesium sulfate heptahydrate Drugs 0.000 claims description 8
- 239000002609 medium Substances 0.000 claims description 8
- CNFDGXZLMLFIJV-UHFFFAOYSA-L manganese(II) chloride tetrahydrate Chemical compound O.O.O.O.[Cl-].[Cl-].[Mn+2] CNFDGXZLMLFIJV-UHFFFAOYSA-L 0.000 claims description 7
- RWVGQQGBQSJDQV-UHFFFAOYSA-M sodium;3-[[4-[(e)-[4-(4-ethoxyanilino)phenyl]-[4-[ethyl-[(3-sulfonatophenyl)methyl]azaniumylidene]-2-methylcyclohexa-2,5-dien-1-ylidene]methyl]-n-ethyl-3-methylanilino]methyl]benzenesulfonate Chemical compound [Na+].C1=CC(OCC)=CC=C1NC1=CC=C(C(=C2C(=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C)C=2C(=CC(=CC=2)N(CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C)C=C1 RWVGQQGBQSJDQV-UHFFFAOYSA-M 0.000 claims description 7
- RZLVQBNCHSJZPX-UHFFFAOYSA-L zinc sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Zn+2].[O-]S([O-])(=O)=O RZLVQBNCHSJZPX-UHFFFAOYSA-L 0.000 claims description 7
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 4
- 235000019797 dipotassium phosphate Nutrition 0.000 claims description 4
- 229910001629 magnesium chloride Inorganic materials 0.000 claims description 4
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 4
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 4
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 4
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims description 4
- 230000001580 bacterial effect Effects 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- 241000894006 Bacteria Species 0.000 abstract description 4
- 239000002994 raw material Substances 0.000 abstract description 2
- 239000010865 sewage Substances 0.000 abstract description 2
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 abstract 1
- 229910052782 aluminium Inorganic materials 0.000 abstract 1
- 210000000697 sensory organ Anatomy 0.000 abstract 1
- 238000010170 biological method Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000003631 expected effect Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 241000124033 Salix Species 0.000 description 1
- 230000031018 biological processes and functions Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000000197 pyrolysis Methods 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 230000001953 sensory effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Water Supply & Treatment (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Environmental & Geological Engineering (AREA)
- Hydrology & Water Resources (AREA)
- Biodiversity & Conservation Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The invention discloses a kind of microorganism High-speed clarification agent administered for black and odorous water, belong to the biological treatment field of sewage.The High-speed clarification agent includes：The weight portion of A liquid 0.5~10, the weight portion of B liquid 1~3 and the weight portion of C liquid 0.1~1；Wherein, A liquid is polyaluminium aluminum water solution；B liquid is indigenous microorganism bacterium solution；C liquid is liquid microelement.The High-speed clarification agent is raw material using river course bed mud in itself, separates the strain of degraded black and odorous water, as B liquid.A liquid and C liquid are added, microorganism High-speed clarification agent is configured as.Black biological High-speed clarification agent is added in black and odorous water, can clarify in 4h black and odorous water, reach comfortable sense organ degree.
Description
Technical Field
The invention belongs to the field of microbial treatment of sewage, and particularly relates to a microbial quick clarifying agent for treating a black and odorous water body of a river and a preparation method thereof.
Background
With the release of the urban black and odorous water body working guide, the treatment of the black and odorous water body has certain standards. Point sources, area sources and endogenous sources can be generally treated by physical, chemical and biological methods. Biological processes are the most economical of all processes. The disadvantage of biological methods is the slow processing speed. Aiming at the defects, the microbial quick clarifying agent is adopted, so that the black and odorous water body can be clarified quickly within 4 hours, and the comfortable transparency is achieved. The invention patent of patent No. 201010610486.2, which provides a method for treating black and odorous river channels and restoring in-situ ecology by microorganisms. The method is characterized in that a substrate, aquatic plants and a compound microbial agent are used for preparing a biological floating island for treatment. Belongs to the endogenous treatment of black and odorous water. The operation is complex and the cost is high. The invention patent of patent No. 201510795860.3 provides a composite microbial preparation for treating black and odorous water body of river, and a preparation method, application and application method thereof. The patent requires high-temperature equipment to prepare the willow block, and the pyrolysis temperature needs to be as high as 180-240 ℃. Not only wastes energy in the manufacturing process, but also has the problem of safety. The microbial preparation has long preparation time, and the microbial preparation has long action time when being put into black and odorous water, so that the expected effect cannot be quickly achieved.
Disclosure of Invention
Based on the problems in the prior art, the invention aims to provide the microbial quick clarifying agent for the black and odorous water body and the preparation method thereof, the operation is simple, the cost is low, and the microbial quick clarifying agent is thrown into the black and odorous water body to quickly clarify the black and odorous water body and quickly achieve the expected effect.
The purpose of the invention is realized by the following technical scheme:
the embodiment of the invention provides a microbial quick clarifying agent for black and odorous water, which comprises the following components in part by weight: 0.5-10 parts of liquid A, 1-3 parts of liquid B and 0.1-1 part of liquid C;
wherein, the solution A is polyaluminium chloride aqueous solution; the solution B is indigenous microbial liquid; the solution C is a trace element solution.
The embodiment of the invention provides a preparation method of a microbial quick clarifying agent for black and odorous water, which is used for preparing the quick clarifying agent and comprises the following steps:
respectively preparing a polyaluminum chloride aqueous solution of the solution A, an indigenous microorganism bacterial solution of the solution B and a trace element liquid of the solution C;
and taking the liquid A, the liquid B and the liquid C according to 0.5-10 parts by weight of the liquid A, 1-3 parts by weight of the liquid B and 0.1-1 part by weight of the liquid C, adding the liquid C into the liquid B during preparation, uniformly stirring, adding the liquid A after 30min, and standing for 1h to obtain the microbial quick clarifying agent for the black and odorous water body.
According to the technical scheme provided by the invention, the microbial quick clarifier for the black and odorous water body provided by the embodiment of the invention is put into the black and odorous water body to quickly clarify the black and odorous water body by matching the polyaluminum chloride aqueous solution of the solution A, the indigenous microbial liquid of the solution B and the trace element liquid of the solution C with reasonable dosage, so that the expected clarifying effect can be quickly achieved.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below with reference to the contents in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments of the present invention without making any creative effort, shall fall within the protection scope of the present invention.
The embodiment of the invention provides a microbial quick clarifying agent for black and odorous water bodies, which is used for quickly clarifying black and odorous water bodies in rivers and comprises the following components: 0.5-10 parts of liquid A, 1-3 parts of liquid B and 0.1-1 part of liquid C;
wherein, the solution A is polyaluminium chloride aqueous solution; the solution B is indigenous microbial liquid; the solution C is a trace element solution. When the clarifier is prepared, the solution C is added into the solution B, the mixture is stirred and uniformly mixed, the solution A is added after 30min, and the mixture is kept stand for 1h to prepare the microbial quick clarifier for the black and odorous water body. When in use, the addition amount of the clarifying agent is 0.01-0.1 m3The microorganism rapidly clarifies/m2A river channel.
In the clarifier, the polyaluminum chloride aqueous solution of the solution A consists of 1 to 10 parts by weight of polyaluminum chloride and 500 to 1000 parts by weight of water.
In the clarifying agent, the trace element liquid of the liquid C consists of: 0.2 to 0.4 part of zinc sulfate heptahydrate, 0.1 to 1 part of manganese chloride tetrahydrate, 0.2 to 0.3 part of sodium molybdate dihydrate and 1000 parts of water.
In the clarifier, the indigenous microbial liquid of the liquid B is prepared by the following method: selecting bottom mud of a river to be treated, inoculating the bottom mud into an indigenous microorganism enrichment culture medium, and performing shaking culture at the rotating speed of 120-180 r/min at the temperature of 28-38 ℃ for 1-3 days to prepare an indigenous microorganism enrichment solution;
inoculating the prepared indigenous microorganism enrichment solution into an indigenous microorganism separation and purification culture medium, and performing shaking table culture at the rotating speed of 120-180 r/min at the temperature of 28-38 ℃ for 3-10 days to prepare an indigenous microorganism purification solution;
and inoculating the prepared indigenous microorganism purified solution into an indigenous microorganism separation and screening culture medium, and performing shake culture at the rotating speed of 120-180 r/min at the temperature of 28-38 ℃ for 1-3 days to obtain a microbial solution, namely the indigenous microbial solution.
In the above clarifying agent, the indigenous microorganism-enriched medium comprises: 1-30 parts of peptone, 1-10 parts of yeast extract, 5-20 parts of sodium chloride and 1000 parts of water; the pH value of the indigenous microorganism enrichment medium is 6-8.
In the above clarifying agent, the culture medium for separation and purification of indigenous microorganisms comprises: 1-30 parts of peptone, 1-10 parts of yeast extract, 5-20 parts of sodium chloride, 0.5-5 parts of ammonium sulfate, 0.1-0.3 part of magnesium sulfate heptahydrate, 0.2-0.6 part of dipotassium phosphate, 0.05-0.4 part of ferrous sulfate heptahydrate, 0.1-4 parts of sodium chloride, 1-5 parts of calcium carbonate and 1000 parts of water, wherein the pH value of the culture medium for separating and purifying the indigenous microorganisms is 6-8.
In the clarifying agent, the culture medium for separating and screening indigenous microorganisms comprises: 1-30 parts of peptone, 1-10 parts of yeast extract, 5-20 parts of sodium chloride, 0.1-3 parts of ammonium sulfate, 0.1-0.3 part of magnesium sulfate heptahydrate, 0.1-0.5 part of dipotassium hydrogen phosphate, 0.01-0.5 part of potassium dihydrogen phosphate, 0.05-0.1 part of ferrous sulfate heptahydrate, 0.1-1 part of sodium chloride, 0.1-0.5 part of magnesium chloride, 1-3 parts of calcium carbonate and 1000 parts of water, wherein the pH value of the culture medium for separating and screening the indigenous microorganisms is 6-8.
The embodiment of the invention also provides a preparation method of the microbial quick clarifying agent for the black and odorous water body, which is used for preparing the quick clarifying agent and comprises the following steps:
respectively preparing a polyaluminum chloride aqueous solution of the solution A, an indigenous microorganism bacterial solution of the solution B and a trace element liquid of the solution C;
and taking the liquid A, the liquid B and the liquid C according to 0.5-10 parts by weight of the liquid A, 1-3 parts by weight of the liquid B and 0.1-1 part by weight of the liquid C, adding the liquid C into the liquid B during preparation, uniformly stirring, adding the liquid A after 30min, and standing for 1h to obtain the microbial quick clarifying agent for the black and odorous water body.
In the preparation method, the polyaluminum chloride aqueous solution of the solution A consists of 1 to 10 parts by weight of polyaluminum chloride and 500 to 1000 parts by weight of water;
the trace element liquid of the liquid C consists of: 0.2-0.4 part of zinc sulfate heptahydrate, 0.1-1 part of manganese chloride tetrahydrate, 0.2-0.3 part of sodium molybdate dihydrate and 1000 parts of water;
the indigenous microbial liquid of the liquid B is prepared by adopting the following method: selecting bottom mud of a river to be treated, inoculating the bottom mud into an indigenous microorganism enrichment culture medium, and performing shaking culture at the rotating speed of 120-180 r/min at the temperature of 28-38 ℃ for 1-3 days to prepare an indigenous microorganism enrichment solution; inoculating the prepared indigenous microorganism enrichment solution into an indigenous microorganism separation and purification culture medium, and performing shaking table culture at the rotating speed of 120-180 r/min at the temperature of 28-38 ℃ for 3-10 days to prepare an indigenous microorganism purification solution; inoculating the prepared indigenous microorganism purified solution into an indigenous microorganism separation screening culture medium, and performing shake culture at the rotating speed of 120-180 r/min at the temperature of 28-38 ℃ for 1-3 days to obtain a microbial solution, namely an indigenous microbial solution; wherein,
the indigenous microorganism-enriched medium comprises: 1-30 parts of peptone, 1-10 parts of yeast extract, 5-20 parts of sodium chloride and 1000 parts of water; the pH value of the indigenous microorganism enrichment medium is 6-8;
the culture medium for separating and purifying the indigenous microorganisms comprises: 1-30 parts of peptone, 1-10 parts of yeast extract, 5-20 parts of sodium chloride, 0.5-5 parts of ammonium sulfate, 0.1-0.3 part of magnesium sulfate heptahydrate, 0.2-0.6 part of dipotassium phosphate, 0.05-0.4 part of ferrous sulfate heptahydrate, 0.1-4 parts of sodium chloride, 1-5 parts of calcium carbonate and 1000 parts of water, wherein the pH value of the culture medium for separating and purifying the indigenous microorganisms is 6-8;
the culture medium for separating and screening indigenous microorganisms comprises: 1-30 parts of peptone, 1-10 parts of yeast extract, 5-20 parts of sodium chloride, 0.1-3 parts of ammonium sulfate, 0.1-0.3 part of magnesium sulfate heptahydrate, 0.1-0.5 part of dipotassium hydrogen phosphate, 0.01-0.5 part of potassium dihydrogen phosphate, 0.05-0.1 part of ferrous sulfate heptahydrate, 0.1-1 part of sodium chloride, 0.1-0.5 part of magnesium chloride, 1-3 parts of calcium carbonate and 1000 parts of water, wherein the pH value of the culture medium for separating and screening the indigenous microorganisms is 6-8.
The addition amount of the prepared clarifying agent is 0.01-0.1 m3The microorganism rapidly clarifies/m2A river channel.
The clarifying agent of the present invention will be described in further detail with reference to specific examples.
Example one
The embodiment provides a microbial quick clarifying agent for black and odorous water, which is prepared by the following steps: preparing the quick clear liquid of the black and odorous water body according to the weight ratio of 10 parts of the liquid A, 3 parts of the liquid B and 1 part of the liquid C.
Wherein, the A liquid is prepared by 10 weight portions of PAC (polyaluminium chloride) and 1000 weight portions of water.
The solution B consists of indigenous microbial bacteria solution. The preparation method comprises the following steps: selecting bottom mud of a river to be treated, inoculating the bottom mud into an indigenous microorganism enrichment culture medium, and carrying out shaking culture at the rotating speed of 180r/min at the temperature of 38 ℃ for 3 days to prepare an indigenous microorganism enrichment solution. Inoculating the indigenous microorganism enrichment solution into an indigenous microorganism separation and purification culture medium, and performing shake culture at 35 ℃ at a rotating speed of 160r/min for 10 days to obtain an indigenous microorganism purification solution. Inoculating the native microorganism purified solution into a native microorganism separation and screening culture medium, and performing shake culture at 28 deg.C and 180r/min for 3 days to obtain native microorganism solution.
The solution C is composed of trace element solution. The preparation method comprises the following steps: 0.2 part of zinc sulfate heptahydrate, 0.1 part of manganese chloride tetrahydrate, 0.2 part of sodium molybdate dihydrate and 1000 parts of water.
Adding a microorganism quick clarifying agent to the black and odorous water body section of a certain river in Beijing, wherein the adding amount is 0.07m3Microbial quick clarifying agent/m2A river channel. The water quality indexes before and after 4h of addition are shown in the following table
Example two
The embodiment provides a microbial quick clarifying agent for black and odorous water, which is prepared by the following steps: preparing the black and odorous water body rapid clarifying solution according to the weight ratio of 5 parts of the liquid A, 1 part of the liquid B and 0.5 part of the liquid C.
Wherein, the A liquid is prepared by 10 weight portions of PAC (polyaluminium chloride) and 1000 weight portions of water.
The solution B consists of indigenous microbial bacteria solution. The preparation method comprises the following steps: selecting bottom mud of a river to be treated, inoculating the bottom mud into an indigenous microorganism enrichment culture medium, and carrying out shaking culture at the rotating speed of 180r/min at the temperature of 38 ℃ for 3 days to prepare an indigenous microorganism enrichment solution. Inoculating the indigenous microorganism enrichment solution into an indigenous microorganism separation and purification culture medium, and performing shake culture at 35 ℃ at a rotating speed of 160r/min for 10 days to obtain an indigenous microorganism purification solution. Inoculating the native microorganism purified solution into a native microorganism separation and screening culture medium, and performing shake culture at 28 deg.C and 180r/min for 3 days to obtain native microorganism solution.
The solution C is composed of trace element solution. The preparation method comprises the following steps: 0.2 part of zinc sulfate heptahydrate, 0.1 part of manganese chloride tetrahydrate, 0.2 part of sodium molybdate dihydrate and 1000 parts of water.
Adding a microorganism quick clarifying agent to the black and odorous water body section of a certain river in Beijing, wherein the adding amount is 0.04m3Microbial quick clarifying agent/m2A river channel. The water quality indexes before and after 4h of addition are shown in the following table
Adding a microorganism quick clarifying agent into the black and odorous water body section of a river of Jilin Changchun, wherein the water quality indexes before and after the addition are shown in the following table
EXAMPLE III
The embodiment provides a microbial quick clarifying agent for black and odorous water, which is prepared by the following steps: preparing the quick clear liquid of the black and odorous water body according to the weight ratio of 7 parts of the liquid A, 1 part of the liquid B and 0.5 part of the liquid C.
Wherein, the A liquid is prepared by 10 weight portions of PAC (polyaluminium chloride) and 1000 weight portions of water.
The B liquid is an indigenous microorganism bacterium liquid, and the preparation method comprises the following steps: selecting bottom mud of a river to be treated, inoculating the bottom mud into an indigenous microorganism enrichment culture medium, and carrying out shaking culture at the rotating speed of 180r/min at the temperature of 38 ℃ for 3 days to prepare an indigenous microorganism enrichment solution. Inoculating the indigenous microorganism enrichment solution into an indigenous microorganism separation and purification culture medium, and performing shake culture at 35 ℃ at a rotating speed of 160r/min for 10 days to obtain an indigenous microorganism purification solution. Inoculating the native microorganism purified solution into a native microorganism separation and screening culture medium, and performing shake culture at 28 deg.C and 180r/min for 3 days to obtain native microorganism solution.
The C liquid is a trace element liquid, and the preparation method comprises the following steps: 0.2 part of zinc sulfate heptahydrate, 0.1 part of manganese chloride tetrahydrate, 0.2 part of sodium molybdate dihydrate and 1000 parts of water.
Adding a microorganism quick clarifying agent to the black and odorous water body section of a certain river in Beijing, wherein the adding amount is 0.04m3Microbial quick clarifying agent/m2A river channel. The water quality indexes before and after 4h of addition are shown in the following table
Adding microorganism fast clarifying agent into black and odorous water body of a certain river section in the north of river, wherein water quality indexes before and after adding are shown in the following table
According to the embodiments, the clarifying agent disclosed by the invention has the advantages that the sediment of the river is used as a raw material, the strain for degrading the black and odorous water body is separated and screened out to be used as the liquid B, the liquid A and the liquid C are added to prepare the microbial quick clarifying agent, the black and odorous water body can be clarified within 4 hours by adding the black biological quick clarifying agent into the black and odorous water body, the comfortable sensory degree is achieved, and the quick clarifying agent has the advantages of simplicity in preparation and operation and good clarifying effect.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention are included in the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
Claims (10)
1. A microbial quick clarifying agent for black and odorous water bodies, which is characterized by comprising the following components in percentage by weight: 0.5-10 parts of liquid A, 1-3 parts of liquid B and 0.1-1 part of liquid C;
wherein, the solution A is polyaluminium chloride aqueous solution; the solution B is indigenous microbial liquid; the solution C is a trace element solution.
2. The microbial quick clarifier for black and odorous water body according to claim 1, wherein the polyaluminium chloride aqueous solution of the solution A comprises 1-10 parts by weight of polyaluminium chloride and 500-1000 parts by weight of water.
3. The microbial quick clarifier for black and odorous water body according to claim 1, wherein the trace element liquid of the liquid C is composed of: 0.2 to 0.4 part of zinc sulfate heptahydrate, 0.1 to 1 part of manganese chloride tetrahydrate, 0.2 to 0.3 part of sodium molybdate dihydrate and 1000 parts of water.
4. The microbial quick clarifier for black and odorous water body according to any one of claims 1 to 3, wherein the indigenous microbial liquid of the liquid B is prepared by the following method: selecting bottom mud of a river to be treated, inoculating the bottom mud into an indigenous microorganism enrichment culture medium, and performing shaking culture at the rotating speed of 120-180 r/min at the temperature of 28-38 ℃ for 1-3 days to prepare an indigenous microorganism enrichment solution;
inoculating the prepared indigenous microorganism enrichment solution into an indigenous microorganism separation and purification culture medium, and performing shaking table culture at the rotating speed of 120-180 r/min at the temperature of 28-38 ℃ for 3-10 days to prepare an indigenous microorganism purification solution;
and inoculating the prepared indigenous microorganism purified solution into an indigenous microorganism separation and screening culture medium, and performing shake culture at the rotating speed of 120-180 r/min at the temperature of 28-38 ℃ for 1-3 days to obtain a microbial solution, namely the indigenous microbial solution.
5. The microbial fast clarifying agent for black and odorous water body according to claim 3, wherein the indigenous microbe-enriched medium includes: 1-30 parts of peptone, 1-10 parts of yeast extract, 5-20 parts of sodium chloride and 1000 parts of water; the pH value of the indigenous microorganism enrichment medium is 6-8.
6. The microbial fast clarifying agent for black and odorous water body according to claim 3, wherein the culture medium for separation and purification of indigenous microbes comprises: 1-30 parts of peptone, 1-10 parts of yeast extract, 5-20 parts of sodium chloride, 0.5-5 parts of ammonium sulfate, 0.1-0.3 part of magnesium sulfate heptahydrate, 0.2-0.6 part of dipotassium phosphate, 0.05-0.4 part of ferrous sulfate heptahydrate, 0.1-4 parts of sodium chloride, 1-5 parts of calcium carbonate and 1000 parts of water, wherein the pH value of the culture medium for separating and purifying the indigenous microorganisms is 6-8.
7. The microbial fast clarifying agent for black and odorous water body according to claim 3, wherein the culture medium for separating and screening indigenous microbes comprises: 1-30 parts of peptone, 1-10 parts of yeast extract, 5-20 parts of sodium chloride, 0.1-3 parts of ammonium sulfate, 0.1-0.3 part of magnesium sulfate heptahydrate, 0.1-0.5 part of dipotassium hydrogen phosphate, 0.01-0.5 part of potassium dihydrogen phosphate, 0.05-0.1 part of ferrous sulfate heptahydrate, 0.1-1 part of sodium chloride, 0.1-0.5 part of magnesium chloride, 1-3 parts of calcium carbonate and 1000 parts of water, wherein the pH value of the culture medium for separating and screening the indigenous microorganisms is 6-8.
8. A method for preparing a microbial quick clarifier for black and odorous water, which is used for preparing the quick clarifier of any one of claims 1 to 7, and comprises the following steps:
respectively preparing a polyaluminum chloride aqueous solution of the solution A, an indigenous microorganism bacterial solution of the solution B and a trace element liquid of the solution C;
and taking the liquid A, the liquid B and the liquid C according to 0.5-10 parts by weight of the liquid A, 1-3 parts by weight of the liquid B and 0.1-1 part by weight of the liquid C, adding the liquid C into the liquid B during preparation, uniformly stirring, adding the liquid A after 30min, and standing for 1h to obtain the microbial quick clarifying agent for the black and odorous water body.
9. The preparation method of the microbial quick clarifier for black and odorous water body according to claim 8, wherein the polyaluminium chloride aqueous solution of solution A is composed of 1-10 parts by weight of polyaluminium chloride and 500-1000 parts by weight of water;
the trace element liquid of the liquid C consists of: 0.2-0.4 part of zinc sulfate heptahydrate, 0.1-1 part of manganese chloride tetrahydrate, 0.2-0.3 part of sodium molybdate dihydrate and 1000 parts of water;
the indigenous microbial liquid of the liquid B is prepared by adopting the following method: selecting bottom mud of a river to be treated, inoculating the bottom mud into an indigenous microorganism enrichment culture medium, and performing shaking culture at the rotating speed of 120-180 r/min at the temperature of 28-38 ℃ for 1-3 days to prepare an indigenous microorganism enrichment solution; inoculating the prepared indigenous microorganism enrichment solution into an indigenous microorganism separation and purification culture medium, and performing shaking table culture at the rotating speed of 120-180 r/min at the temperature of 28-38 ℃ for 3-10 days to prepare an indigenous microorganism purification solution; inoculating the prepared indigenous microorganism purified solution into an indigenous microorganism separation screening culture medium, and performing shake culture at the rotating speed of 120-180 r/min at the temperature of 28-38 ℃ for 1-3 days to obtain a microbial solution, namely an indigenous microbial solution; wherein,
the indigenous microorganism-enriched medium comprises: 1-30 parts of peptone, 1-10 parts of yeast extract, 5-20 parts of sodium chloride and 1000 parts of water; the pH value of the indigenous microorganism enrichment medium is 6-8;
the culture medium for separating and purifying the indigenous microorganisms comprises: 1-30 parts of peptone, 1-10 parts of yeast extract, 5-20 parts of sodium chloride, 0.5-5 parts of ammonium sulfate, 0.1-0.3 part of magnesium sulfate heptahydrate, 0.2-0.6 part of dipotassium phosphate, 0.05-0.4 part of ferrous sulfate heptahydrate, 0.1-4 parts of sodium chloride, 1-5 parts of calcium carbonate and 1000 parts of water, wherein the pH value of the culture medium for separating and purifying the indigenous microorganisms is 6-8;
the culture medium for separating and screening indigenous microorganisms comprises: 1-30 parts of peptone, 1-10 parts of yeast extract, 5-20 parts of sodium chloride, 0.1-3 parts of ammonium sulfate, 0.1-0.3 part of magnesium sulfate heptahydrate, 0.1-0.5 part of dipotassium hydrogen phosphate, 0.01-0.5 part of potassium dihydrogen phosphate, 0.05-0.1 part of ferrous sulfate heptahydrate, 0.1-1 part of sodium chloride, 0.1-0.5 part of magnesium chloride, 1-3 parts of calcium carbonate and 1000 parts of water, wherein the pH value of the culture medium for separating and screening the indigenous microorganisms is 6-8.
10. The method for preparing the microbial quick clarifier for black and odorous water body according to claim 8 or 9, wherein the addition amount of the microbial quick clarifier for black and odorous water body is 0.01-0.1 m3The microorganism rapidly clarifies/m2A river channel.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108101231A (en) * | 2017-12-27 | 2018-06-01 | 桑德生态科技有限公司 | A kind of black and odorous water administers agent and method |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102050523A (en) * | 2010-12-29 | 2011-05-11 | 赵亚勋 | Method for treatment and in-situ ecological restoration of black and stink riverway by using microorganism |
| CN102583881A (en) * | 2012-02-13 | 2012-07-18 | 广东中大环保科技投资有限公司 | Method for treating water quality of black-odor river channel |
| CN103911332A (en) * | 2014-04-04 | 2014-07-09 | 范敏 | Method for culturing nitrogen and phosphorus removal composite bacteria of black and odorous water body |
| CN103922489A (en) * | 2014-05-09 | 2014-07-16 | 四川清和科技有限公司 | Microorganism composite flocculant coagulation bacterium application method |
| CN105174471A (en) * | 2015-09-16 | 2015-12-23 | 赵亚勋 | Reinforced black odorous river microecological reconstruction/balance and water quality improvement method |
| CN105417727A (en) * | 2015-12-21 | 2016-03-23 | 南京领先环保技术股份有限公司 | Method for deeply treating micro-polluted source water through in-situ enrichment, immobilization and acclimatization of indigenous microorganisms |
-
2016
- 2016-12-16 CN CN201611168091.5A patent/CN106745804B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102050523A (en) * | 2010-12-29 | 2011-05-11 | 赵亚勋 | Method for treatment and in-situ ecological restoration of black and stink riverway by using microorganism |
| CN102583881A (en) * | 2012-02-13 | 2012-07-18 | 广东中大环保科技投资有限公司 | Method for treating water quality of black-odor river channel |
| CN103911332A (en) * | 2014-04-04 | 2014-07-09 | 范敏 | Method for culturing nitrogen and phosphorus removal composite bacteria of black and odorous water body |
| CN103922489A (en) * | 2014-05-09 | 2014-07-16 | 四川清和科技有限公司 | Microorganism composite flocculant coagulation bacterium application method |
| CN105174471A (en) * | 2015-09-16 | 2015-12-23 | 赵亚勋 | Reinforced black odorous river microecological reconstruction/balance and water quality improvement method |
| CN105417727A (en) * | 2015-12-21 | 2016-03-23 | 南京领先环保技术股份有限公司 | Method for deeply treating micro-polluted source water through in-situ enrichment, immobilization and acclimatization of indigenous microorganisms |
Non-Patent Citations (1)
| Title |
|---|
| 王伟东等主编: "《生物制药工艺学》", 31 August 2015 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108101231A (en) * | 2017-12-27 | 2018-06-01 | 桑德生态科技有限公司 | A kind of black and odorous water administers agent and method |
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