CN106745804A - A kind of microorganism High-speed clarification agent for black and odorous water and preparation method thereof - Google Patents

A kind of microorganism High-speed clarification agent for black and odorous water and preparation method thereof Download PDF

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Publication number
CN106745804A
CN106745804A CN201611168091.5A CN201611168091A CN106745804A CN 106745804 A CN106745804 A CN 106745804A CN 201611168091 A CN201611168091 A CN 201611168091A CN 106745804 A CN106745804 A CN 106745804A
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weight portion
liquid
microorganism
water
black
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CN106745804B (en
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屈佳玉
马林
刘金泉
王凯
任彦强
邢成良
张利君
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Beijing Sound Environmental Engineering Co Ltd
Beijing Epure International Water Co Ltd
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Beijing Sound Environmental Engineering Co Ltd
Beijing Epure International Water Co Ltd
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    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/34Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor

Abstract

The invention discloses a kind of microorganism High-speed clarification agent administered for black and odorous water, belong to the biological treatment field of sewage.The High-speed clarification agent includes:The weight portion of A liquid 0.5~10, the weight portion of B liquid 1~3 and the weight portion of C liquid 0.1~1;Wherein, A liquid is polyaluminium aluminum water solution;B liquid is indigenous microorganism bacterium solution;C liquid is liquid microelement.The High-speed clarification agent is raw material using river course bed mud in itself, separates the strain of degraded black and odorous water, as B liquid.A liquid and C liquid are added, microorganism High-speed clarification agent is configured as.Black biological High-speed clarification agent is added in black and odorous water, can clarify in 4h black and odorous water, reach comfortable sense organ degree.

Description

A kind of microorganism High-speed clarification agent for black and odorous water and preparation method thereof
Technical field
The invention belongs to the microbiological treatment field of sewage, more particularly to a kind of micro- life administered for river black and odorous water Thing High-speed clarification agent and preparation method thereof.
Background technology
With《City black and odorous water operating instructions》Issue, the improvement of black and odorous water has certain standard.Typically may be used To point source, face source and endogenous to be administered using Physical, chemical method and bioanalysis.Bioanalysis is most passed through in all methods The method of Ji.The shortcoming of bioanalysis is slow processing speed.For disadvantage mentioned above, using microorganism High-speed clarification agent, can in 4h Make black and odorous water High-speed clarification, reach comfortable transparency.The patent of invention of the patent No. 201010610486.2, the patent is provided A kind of microorganism is to black-odor riverway treatment and the method for restoration of the ecosystem in situ.It is using matrix, water plant and complex microorganism Microbial inoculum is processed to be made the mode of biological floating island.Belong to the endogenous improvement of black and odorous water.Complex operation, it is relatively costly. The patent of invention of the patent No. 201510795860.3, the patent provides a kind of complex microorganism administered for river black and odorous water Preparation and preparation method, using and methods for using them.The patent needs high-temperature service to prepare willow block, and the temperature of pyrolysis needs height Up to 180~240 DEG C.The energy is not only wasted in manufacturing process, and has safety issue.Prepare time of microorganism formulation compared with It is long, and the onset time is also more long in being put into black and odorous water, it is impossible to it is rapidly achieved Expected Results.
The content of the invention
It is fast it is an object of the invention to provide a kind of microorganism for black and odorous water based on the problems of prior art Fast fining agent and preparation method thereof, simple to operate, cost is relatively low, enables black and odorous water High-speed clarification in input black and odorous water, soon Speed produces a desired effect.
The purpose of the present invention is achieved through the following technical solutions:
The embodiment of the present invention provides a kind of microorganism High-speed clarification agent for black and odorous water, and the High-speed clarification agent includes:A The weight portion of liquid 0.5~10, the weight portion of B liquid 1~3 and the weight portion of C liquid 0.1~1;
Wherein, A liquid is polyaluminium aluminum water solution;B liquid is indigenous microorganism bacterium solution;C liquid is liquid microelement.
The embodiment of the present invention provides a kind of preparation method of microorganism High-speed clarification agent for black and odorous water, for preparing High-speed clarification agent of the present invention, comprises the following steps:
The liquid microelement of the polyaluminium aluminum water solution of A liquid, the indigenous microorganism bacterium solution of B liquid and C liquid is prepared respectively;
A liquid, B liquid and C liquid are taken by the weight portion of A liquid 0.5~10, the weight portion of B liquid 1~3 and the weight portion of C liquid 0.1~1, is prepared When, during C liquid first added into B liquid, stir and evenly mix, A liquid is added after 30min, stand the micro- life being obtained after 1h for black and odorous water Thing High-speed clarification agent.
As seen from the above technical solution provided by the invention, it is provided in an embodiment of the present invention for the micro- of black and odorous water Biological High-speed clarification agent, by the polyaluminium aluminum water solution of the A liquid of reasonable volume, the indigenous microorganism bacterium solution of B liquid and C liquid Liquid microelement coordinates, and black and odorous water High-speed clarification is enable in input black and odorous water, can be rapidly achieved expected clarifying effect, and this is clear Clear agent preparation manipulation is simple, and cost is relatively low.
Specific embodiment
With reference to the content in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete Ground description, it is clear that described embodiment is only a part of embodiment of the invention, rather than whole embodiments.Based on this Inventive embodiment, the every other implementation that those of ordinary skill in the art are obtained under the premise of creative work is not made Example, belongs to protection scope of the present invention.
The embodiment of the present invention provides a kind of microorganism High-speed clarification agent for black and odorous water, for river black and odorous water High-speed clarification, the High-speed clarification agent includes:The weight portion of A liquid 0.5~10, the weight portion of B liquid 1~3 and the weight portion of C liquid 0.1~1;
Wherein, A liquid is polyaluminium aluminum water solution;B liquid is indigenous microorganism bacterium solution;C liquid is liquid microelement.The clarification When agent is prepared, during C liquid first added into B liquid, stir and evenly mix, A liquid is added after 30min, be obtained for black and odorous water after standing 1h Microorganism High-speed clarification agent.When using, the dosage of the fining agent is 0.01~0.1m3The microorganism High-speed clarification agent/m2River Road.
In above-mentioned fining agent, the polyaluminium aluminum water solution of A liquid by the aluminium polychloride of 1~10 weight portion and 500~ The water composition of 1000 weight portions.
In above-mentioned fining agent, the liquid microelement of C liquid by:The weight portion of white vitriol 0.2~0.4, tetrahydrate manganese chloride 0.1 ~1 weight portion, the weight portion of Sodium Molybdate Dihydrate 0.2~0.3 and the weight portion of water 1000 are constituted.
In above-mentioned fining agent, the indigenous microorganism bacterium solution of B liquid is obtained in the following ways:Choose the bottom in river course to be administered Mud, accesses in indigenous microorganism enriched medium, and the rotating speed of 120~180r/min carries out shaking table culture under the conditions of 28~38 DEG C 1~3 day, indigenous microorganism pregnant solution is obtained;
The indigenous microorganism pregnant solution by obtained in is accessed in indigenous microorganism isolation and purification culture base, and 28~38 The rotating speed of 120~180r/min carries out shaking table culture 3~10 days under the conditions of DEG C, and indigenous microorganism refined solution is obtained;
By obtained indigenous microorganism refined solution access indigenous microorganism separation screening culture medium in, in 28~38 DEG C of conditions The rotating speed of lower 120~180r/min carries out microbial inoculum as indigenous microorganism bacterium solution obtained in shaking table culture 1~3 day.
In above-mentioned fining agent, indigenous microorganism enriched medium includes:The weight portion of peptone 1~30, the weight of yeast extract 1~10 Amount part, the weight portion of sodium chloride 5~20 and the weight portion of water 1000;The pH value of the indigenous microorganism enriched medium is 6~8.
In above-mentioned fining agent, indigenous microorganism isolation and purification culture base includes:The weight portion of peptone 1~30, yeast extract 1~ 10 weight portions, the weight portion of sodium chloride 5~20, the weight portion of ammonium sulfate 0.5~5, the weight portion of epsom salt 0.1~0.3, phosphoric acid hydrogen The weight portion of dipotassium 0.2~0.6, the weight portion of ferrous sulfate heptahydrate 0.05~0.4, the weight portion of sodium chloride 0.1~4, calcium carbonate 1~5 Weight portion and the weight portion of water 1000, the pH value of the indigenous microorganism isolation and purification culture base is 6~8.
In above-mentioned fining agent, indigenous microorganism separation screening culture medium includes:The weight portion of peptone 1~30, yeast extract 1~ 10 weight portions, the weight portion of sodium chloride 5~20, the weight portion of ammonium sulfate 0.1~3, the weight portion of epsom salt 0.1~0.3, phosphoric acid hydrogen The weight portion of dipotassium 0.1~0.5, the weight portion of potassium dihydrogen phosphate 0.01~0.5, the weight portion of ferrous sulfate heptahydrate 0.05~0.1, chlorination The weight portion of sodium 0.1~1, the weight portion of magnesium chloride 0.1~0.5, the weight portion of calcium carbonate 1~3 and the weight portion of water 1000, indigenous micro- life The pH value of thing separation screening culture medium is 6~8.
The embodiment of the present invention also provides a kind of preparation method of the microorganism High-speed clarification agent for black and odorous water, for making Standby above-mentioned High-speed clarification agent, comprises the following steps:
The liquid microelement of the polyaluminium aluminum water solution of A liquid, the indigenous microorganism bacterium solution of B liquid and C liquid is prepared respectively;
A liquid, B liquid and C liquid are taken by the weight portion of A liquid 0.5~10, the weight portion of B liquid 1~3 and the weight portion of C liquid 0.1~1, is prepared When, during C liquid first added into B liquid, stir and evenly mix, A liquid is added after 30min, stand the micro- life being obtained after 1h for black and odorous water Thing High-speed clarification agent.
In above-mentioned preparation method, the polyaluminium aluminum water solution of A liquid by the aluminium polychloride of 1~10 weight portion and 500~ The water composition of 1000 weight portions;
The liquid microelement of the C liquid by:The weight portion of white vitriol 0.2~0.4, the weight of tetrahydrate manganese chloride 0.1~1 Part, the weight portion of Sodium Molybdate Dihydrate 0.2~0.3 and the weight portion of water 1000 are constituted;
The indigenous microorganism bacterium solution of the B liquid is obtained in the following ways:The bed mud in river course to be administered is chosen, original inhabitants are accessed In microorganism enriched medium, the rotating speed of 120~180r/min carries out shaking table culture 1~3 day under the conditions of 28~38 DEG C, is obtained Indigenous microorganism pregnant solution;The indigenous microorganism pregnant solution by obtained in is accessed in indigenous microorganism isolation and purification culture base, And the rotating speed of 120~180r/min carries out shaking table culture 3~10 days under the conditions of 28~38 DEG C, indigenous microorganism purifying is obtained Liquid;By obtained indigenous microorganism refined solution access indigenous microorganism separation screening culture medium in, 120 under the conditions of 28~38 DEG C The rotating speed of~180r/min carries out microbial inoculum as indigenous microorganism bacterium solution obtained in shaking table culture 1~3 day;Wherein,
The indigenous microorganism enriched medium includes:The weight portion of peptone 1~30, the weight portion of yeast extract 1~10, chlorination The weight portion of sodium 5~20 and the weight portion of water 1000;The pH value of the indigenous microorganism enriched medium is 6~8;
The indigenous microorganism isolation and purification culture base includes:The weight portion of peptone 1~30, the weight portion of yeast extract 1~10, The weight portion of sodium chloride 5~20, the weight portion of ammonium sulfate 0.5~5, the weight portion of epsom salt 0.1~0.3, dipotassium hydrogen phosphate 0.2~ 0.6 weight portion, the weight portion of ferrous sulfate heptahydrate 0.05~0.4, the weight portion of sodium chloride 0.1~4, the weight portion of calcium carbonate 1~5 and water 1000 weight portions, the pH value of the indigenous microorganism isolation and purification culture base is 6~8;
The indigenous microorganism separation screening culture medium includes:The weight portion of peptone 1~30, the weight portion of yeast extract 1~10, The weight portion of sodium chloride 5~20, the weight portion of ammonium sulfate 0.1~3, the weight portion of epsom salt 0.1~0.3, dipotassium hydrogen phosphate 0.1~ 0.5 weight portion, the weight portion of potassium dihydrogen phosphate 0.01~0.5, the weight portion of ferrous sulfate heptahydrate 0.05~0.1, sodium chloride 0.1~1 Weight portion, the weight portion of magnesium chloride 0.1~0.5, the weight portion of calcium carbonate 1~3 and the weight portion of water 1000, the indigenous microorganism bolter The pH value for selecting culture medium is 6~8.
The above-mentioned dosage for preparing fining agent is 0.01~0.1m3The microorganism High-speed clarification agent/m2River course.
Fining agent of the invention is described in further detail below in conjunction with specific embodiment.
Embodiment one
The present embodiment provides a kind of microorganism High-speed clarification agent for black and odorous water, and the fining agent preparation method is as follows: According to the weight portion of A liquid 10, the weight portion of B liquid 3, the weight of the weight portion of C liquid 1 is than configuration black and odorous water High-speed clarification liquid.
Wherein, by 10 weight portion PAC (aluminium polychloride), the weight portion of water 1000 is obtained A liquid.
B liquid is made up of indigenous microorganism bacterium solution.Preparation method:The bed mud in river course to be administered is chosen, indigenous microorganism is accessed In enriched medium, the rotating speed of 180r/min carries out shaking table culture 3 days under the conditions of 38 DEG C, and indigenous microorganism pregnant solution is obtained. By indigenous microorganism pregnant solution access indigenous microorganism isolation and purification culture base in, and under the conditions of 35 DEG C 160r/min rotating speed Carry out shaking table culture 10 days, indigenous microorganism refined solution is obtained.Indigenous microorganism refined solution is accessed into indigenous microorganism bolter Select in culture medium, the rotating speed of 180r/min carries out shaking table culture 3 days under the conditions of 28 DEG C, indigenous microorganism bacterium solution is obtained.
C liquid is made up of liquid microelement.Preparation method:The weight portion of white vitriol 0.2, the weight portion of tetrahydrate manganese chloride 0.1, The weight portion of Sodium Molybdate Dihydrate 0.2, the weight portion of water 1000.
Microorganism High-speed clarification agent is added to Beijing river black and odorous water section, dosage is 0.07m3Microorganism High-speed clarification Agent/m2River course.Water quality index see the table below before adding and after adding 4h
Embodiment two
The present embodiment provides a kind of microorganism High-speed clarification agent for black and odorous water, and the fining agent preparation method is as follows: According to the weight portion of A liquid 5, the weight portion of B liquid 1, the weight of the weight portion of C liquid 0.5 is than configuration black and odorous water High-speed clarification liquid.
Wherein, by 10 weight portion PAC (aluminium polychloride), the weight portion of water 1000 is obtained A liquid.
B liquid is made up of indigenous microorganism bacterium solution.Preparation method:The bed mud in river course to be administered is chosen, indigenous microorganism is accessed In enriched medium, the rotating speed of 180r/min carries out shaking table culture 3 days under the conditions of 38 DEG C, and indigenous microorganism pregnant solution is obtained. By indigenous microorganism pregnant solution access indigenous microorganism isolation and purification culture base in, and under the conditions of 35 DEG C 160r/min rotating speed Carry out shaking table culture 10 days, indigenous microorganism refined solution is obtained.Indigenous microorganism refined solution is accessed into indigenous microorganism bolter Select in culture medium, the rotating speed of 180r/min carries out shaking table culture 3 days under the conditions of 28 DEG C, indigenous microorganism bacterium solution is obtained.
C liquid is made up of liquid microelement.Preparation method:The weight portion of white vitriol 0.2, the weight portion of tetrahydrate manganese chloride 0.1, The weight portion of Sodium Molybdate Dihydrate 0.2, the weight portion of water 1000.
Microorganism High-speed clarification agent is added to Beijing river black and odorous water section, dosage is 0.04m3Microorganism High-speed clarification Agent/m2River course.Water quality index see the table below before adding and after adding 4h
Microorganism High-speed clarification agent is added to Jilin Changchun river black and odorous water section, before adding and rear water quality index is added and is seen Following table
Embodiment three
The present embodiment provides a kind of microorganism High-speed clarification agent for black and odorous water, and the fining agent preparation method is as follows: According to the weight portion of A liquid 7, the weight portion of B liquid 1, the weight of the weight portion of C liquid 0.5 is than configuration black and odorous water High-speed clarification liquid.
Wherein, A liquid is obtained by 10 weight portion PAC (aluminium polychloride) and the weight portion of water 1000.
B liquid is indigenous microorganism bacterium solution, and its preparation method is:The bed mud in river course to be administered is chosen, indigenous microorganism is accessed In enriched medium, the rotating speed of 180r/min carries out shaking table culture 3 days under the conditions of 38 DEG C, and indigenous microorganism pregnant solution is obtained. By indigenous microorganism pregnant solution access indigenous microorganism isolation and purification culture base in, and under the conditions of 35 DEG C 160r/min rotating speed Carry out shaking table culture 10 days, indigenous microorganism refined solution is obtained.Indigenous microorganism refined solution is accessed into indigenous microorganism bolter Select in culture medium, the rotating speed of 180r/min carries out shaking table culture 3 days under the conditions of 28 DEG C, indigenous microorganism bacterium solution is obtained.
C liquid is liquid microelement, and its preparation method is:The weight portion of white vitriol 0.2, the weight portion of tetrahydrate manganese chloride 0.1, The weight portion of Sodium Molybdate Dihydrate 0.2, the weight portion of water 1000.
Microorganism High-speed clarification agent is added to Beijing river black and odorous water section, dosage is 0.04m3Microorganism High-speed clarification Agent/m2River course.Water quality index see the table below before adding and after adding 4h
Microorganism High-speed clarification agent is added to Hebei section black and odorous water, before adding and rear water quality index is added and be see the table below
By the various embodiments described above as can be seen that fining agent of the invention is raw material using river course bed mud in itself, point From the strain of degraded black and odorous water is filtered out as B liquid, A liquid and C liquid are added, microorganism High-speed clarification agent is configured as, black Black biological High-speed clarification agent is added in smelly water body, can clarify in 4h black and odorous water, reach comfortable sense organ degree, this is quick clear Clear black and odorous water, with preparation and simple to operate, the good advantage of clarifying effect.
The above, the only present invention preferably specific embodiment, but protection scope of the present invention is not limited thereto, Any one skilled in the art in the technical scope of present disclosure, the change or replacement that can be readily occurred in, Should all be included within the scope of the present invention.Therefore, protection scope of the present invention should be with the protection model of claims Enclose and be defined.

Claims (10)

1. a kind of microorganism High-speed clarification agent for black and odorous water, it is characterised in that the High-speed clarification agent includes:A liquid 0.5~ 10 weight portions, the weight portion of B liquid 1~3 and the weight portion of C liquid 0.1~1;
Wherein, A liquid is polyaluminium aluminum water solution;B liquid is indigenous microorganism bacterium solution;C liquid is liquid microelement.
2. a kind of microorganism High-speed clarification agent for black and odorous water according to claim 1, it is characterised in that the A The polyaluminium aluminum water solution of liquid is made up of the aluminium polychloride of 1~10 weight portion with the water of 500~1000 weight portions.
3. a kind of microorganism High-speed clarification agent for black and odorous water as claimed in claim 1, it is characterised in that the C liquid Liquid microelement by:The weight portion of white vitriol 0.2~0.4, the weight portion of tetrahydrate manganese chloride 0.1~1, Sodium Molybdate Dihydrate 0.2 ~0.3 weight portion and the weight portion of water 1000 are constituted.
4. a kind of microorganism High-speed clarification agent for black and odorous water according to any one of claims 1 to 3, its feature exists In the indigenous microorganism bacterium solution of the B liquid is obtained in the following ways:The bed mud in river course to be administered is chosen, indigenous micro- life is accessed In thing enriched medium, the rotating speed of 120~180r/min carries out shaking table culture 1~3 day under the conditions of 28~38 DEG C, and original inhabitants are obtained Enrichment by Microorganisms liquid collecting;
The indigenous microorganism pregnant solution by obtained in is accessed in indigenous microorganism isolation and purification culture base, and in 28~38 DEG C of bars The rotating speed of 120~180r/min carries out shaking table culture 3~10 days under part, and indigenous microorganism refined solution is obtained;
By obtained indigenous microorganism refined solution access indigenous microorganism separation screening culture medium in, under the conditions of 28~38 DEG C The rotating speed of 120~180r/min carries out microbial inoculum as indigenous microorganism bacterium solution obtained in shaking table culture 1~3 day.
5. a kind of microorganism High-speed clarification agent for black and odorous water as claimed in claim 3, the indigenous microorganism enrichment Culture medium includes:The weight portion of peptone 1~30, the weight portion of yeast extract 1~10, the weight portion of sodium chloride 5~20 and the weight of water 1000 Part;The pH value of the indigenous microorganism enriched medium is 6~8.
6. a kind of microorganism High-speed clarification agent for black and odorous water as claimed in claim 3, it is characterised in that the original inhabitants Microorganism isolation and purification culture base includes:The weight portion of peptone 1~30, the weight portion of yeast extract 1~10, the weight of sodium chloride 5~20 Part, the weight portion of ammonium sulfate 0.5~5, the weight portion of epsom salt 0.1~0.3, the weight portion of dipotassium hydrogen phosphate 0.2~0.6, seven water The weight portion of ferrous sulfate 0.05~0.4, the weight portion of sodium chloride 0.1~4, the weight portion of calcium carbonate 1~5 and the weight portion of water 1000, should The pH value of indigenous microorganism isolation and purification culture base is 6~8.
7. a kind of microorganism High-speed clarification agent for black and odorous water as claimed in claim 3, it is characterised in that the original inhabitants Microorganism separation screening culture medium includes:The weight portion of peptone 1~30, the weight portion of yeast extract 1~10, the weight of sodium chloride 5~20 Part, the weight portion of ammonium sulfate 0.1~3, the weight portion of epsom salt 0.1~0.3, the weight portion of dipotassium hydrogen phosphate 0.1~0.5, phosphoric acid The weight portion of potassium dihydrogen 0.01~0.5, the weight portion of ferrous sulfate heptahydrate 0.05~0.1, the weight portion of sodium chloride 0.1~1, magnesium chloride 0.1~0.5 weight portion, the weight portion of calcium carbonate 1~3 and the weight portion of water 1000, the pH of the indigenous microorganism separation screening culture medium Be worth is 6~8.
8. the preparation method of a kind of microorganism High-speed clarification agent for black and odorous water, it is characterised in that will for preparing right The High-speed clarification agent described in 1 to 7 any one is asked, is comprised the following steps:
The liquid microelement of the polyaluminium aluminum water solution of A liquid, the indigenous microorganism bacterium solution of B liquid and C liquid is prepared respectively;
A liquid, B liquid and C liquid are taken by the weight portion of A liquid 0.5~10, the weight portion of B liquid 1~3 and the weight portion of C liquid 0.1~1, during preparation, will C liquid is first added in B liquid, is stirred and evenly mixed, and A liquid is added after 30min, is obtained after standing 1h quick for the microorganism of black and odorous water Fining agent.
9. a kind of preparation method of microorganism High-speed clarification agent for black and odorous water according to claim 8, its feature It is that the polyaluminium aluminum water solution of the A liquid is by the aluminium polychloride of 1~10 weight portion and the water of 500~1000 weight portions Composition;
The liquid microelement of the C liquid by:The weight portion of white vitriol 0.2~0.4, the weight portion of tetrahydrate manganese chloride 0.1~1, two The weight portion of water sodium molybdate 0.2~0.3 and the weight portion of water 1000 are constituted;
The indigenous microorganism bacterium solution of the B liquid is obtained in the following ways:The bed mud in river course to be administered is chosen, indigenous micro- life is accessed In thing enriched medium, the rotating speed of 120~180r/min carries out shaking table culture 1~3 day under the conditions of 28~38 DEG C, and original inhabitants are obtained Enrichment by Microorganisms liquid collecting;The indigenous microorganism pregnant solution by obtained in is accessed in indigenous microorganism isolation and purification culture base, and The rotating speed of 120~180r/min carries out shaking table culture 3~10 days under the conditions of 28~38 DEG C, and indigenous microorganism refined solution is obtained;Will Obtained indigenous microorganism refined solution is accessed in indigenous microorganism separation screening culture medium, 120 under the conditions of 28~38 DEG C~ The rotating speed of 180r/min carries out microbial inoculum as indigenous microorganism bacterium solution obtained in shaking table culture 1~3 day;Wherein,
The indigenous microorganism enriched medium includes:The weight portion of peptone 1~30, the weight portion of yeast extract 1~10, sodium chloride 5 ~20 weight portions and the weight portion of water 1000;The pH value of the indigenous microorganism enriched medium is 6~8;
The indigenous microorganism isolation and purification culture base includes:The weight portion of peptone 1~30, the weight portion of yeast extract 1~10, chlorination The weight portion of sodium 5~20, the weight portion of ammonium sulfate 0.5~5, the weight portion of epsom salt 0.1~0.3, dipotassium hydrogen phosphate 0.2~0.6 Weight portion, the weight portion of ferrous sulfate heptahydrate 0.05~0.4, the weight portion of sodium chloride 0.1~4, the weight portion of calcium carbonate 1~5 and water 1000 weight portions, the pH value of the indigenous microorganism isolation and purification culture base is 6~8;
The indigenous microorganism separation screening culture medium includes:The weight portion of peptone 1~30, the weight portion of yeast extract 1~10, chlorination The weight portion of sodium 5~20, the weight portion of ammonium sulfate 0.1~3, the weight portion of epsom salt 0.1~0.3, dipotassium hydrogen phosphate 0.1~0.5 Weight portion, the weight portion of potassium dihydrogen phosphate 0.01~0.5, the weight portion of ferrous sulfate heptahydrate 0.05~0.1, the weight of sodium chloride 0.1~1 Part, the weight portion of magnesium chloride 0.1~0.5, the weight portion of calcium carbonate 1~3 and the weight portion of water 1000, indigenous microorganism separation screening training The pH value for supporting base is 6~8.
10. the preparation method of a kind of microorganism High-speed clarification agent for black and odorous water according to claim 8 or claim 9, its It is characterised by, the dosage of the microorganism High-speed clarification agent for black and odorous water is 0.01~0.1m3The microorganism is quick Fining agent/m2River course.
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CN108101231A (en) * 2017-12-27 2018-06-01 桑德生态科技有限公司 A kind of black and odorous water administers agent and method

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