CN103923868A - Mixed flora microbial preparation and application thereof in sewage treatment - Google Patents
Mixed flora microbial preparation and application thereof in sewage treatment Download PDFInfo
- Publication number
- CN103923868A CN103923868A CN201410181469.XA CN201410181469A CN103923868A CN 103923868 A CN103923868 A CN 103923868A CN 201410181469 A CN201410181469 A CN 201410181469A CN 103923868 A CN103923868 A CN 103923868A
- Authority
- CN
- China
- Prior art keywords
- water
- microbial preparation
- sewage
- pseudomonas
- mixed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
The invention discloses a mixed flora microbial preparation which is a bacterium suspension consisting of paenibacillus, pseudomonas stutzeri, pseudomonas pseudoalcaligenes and pseudomonas oleovorans, wherein the total viable count is over 3.8*10<8> CFU/ml. The mixed flora microbial preparation can be used for treating sewage, and can enhance the capability of treating polluted river water in autumn artificial wetlands and municipal domestic sewage. When the mixed flora microbial preparation is specifically applied, the method comprises the step of adding the mixed flora microbial preparation into an autumn artificial wetland system, wherein the adding amount of the preparation is 1% of the volume of the sewage, and the treatment time is 7 days or more. Through detection, after the sewage is treated by adopting the mixed flora microbial preparation for 7 days, the COD (chemical oxygen demand) and content of total nitrogen and total phosphorus of the effluent of the treated polluted river water reach III-class water quality standards of Environmental Quality Standards for Surface Water, and the COD and content of total nitrogen and total phosphorus of the effluent of the treated municipal domestic sewage reach first-grade B standards of Discharge Standards of Pollutants for Municipal Wastewater Treatment Plant.
Description
Technical field
The present invention relates to a kind of mixed bacterial microbial preparation, and the application in disposing of sewage, especially the application in the ability of reinforcement artificial swamp in autumn processing polluted river water and town domestic sewage, belongs to environmental technology field.
Background technology
Artificial swamp is a kind of by artificial construction and supervision and control, and with the similar ground of swampland, it utilizes physics, chemistry and biological triple synergies in natural ecosystems to realize dirty water purification.According to foreign data, construction costs and the working cost of general artificial swamp are only 1/10～1/2 of traditional secondary activated sludge process, and unit sewage power consumption and sewage disposal expense are starkly lower than traditional wastewater treatment plant.In artificial wet land system, the activity of microorganism is the principal element of wastewater through organic matter degradation, is main biotic population and the undertaker of absorption and degradation of contaminant.What play a major role aspect the removal of nitrogen, be Ammonifying bacteria, nitrite bacteria and denitrifying bacterium.The removal of phosphorus is absorbing and the absorption of Wetland Substrate by plant and part microorganism mainly.
Summer Plants of Constructed Wetlands riotous growth, in water body microbial activities vigorous, the organism in can better utilised sewage, nitrogen phosphoric, has good cleaning action to sewage.Yet during autumn, wetland plant is ripe, and growth metabolism weakens, and the sorption of nitrogen phosphoric is weakened.While is along with the reduction of temperature, in wet land system, the metabolism of microorganism weakens, therefore in the fall by increasing the quantity of plant rhizosphere superior microorganism, adopting microbial technique strengthened artificial wet land to strengthen Pollution abatement dynamics, is the optimal path that improves removal efficiency of nitrogen and phosphorus, saves pollution treatment cost.The quantity that directly increases rhizospheric microorganism has become the development trend that improves wetland treatment effect.
Summary of the invention
For above-mentioned prior art, in view of the wide application prospect of microbial augmentation artificial wetland process in sewage disposal, the invention provides a kind of mixed bacterial microbial preparation, and the application in the ability of reinforcement artificial swamp in autumn processing polluted river water and town domestic sewage.
The present invention is achieved by the following technical solutions:
A mixed bacterial microbial preparation, the bacteria suspension being formed by series bacillus, Pseudomonas stutzeri, pseudomonas pseudoalcaligenes and Pseudomonas oleovorans, total viable count of four kinds of bacterium is 3.8 * 10
8more than CFU/ml.
Further, described series bacillus is selected from the bacterial classification that deposit number is CCTCC NO:M2011120, this bacterium classification called after: Paenibacillus sp.XP1, preservation date is on April 10th, 2011, depositary institution is: Chinese Typical Representative culture collection center, preservation address is: China. Wuhan. and Wuhan University, postcode 430072.Be documented in another Chinese patent of applicant the patent No. 201110121394.2, publication number 102220265A.
Further, described Pseudomonas stutzeri is selected from the bacterial classification that deposit number is CCTCC NO:M2011431, this bacterium classification called after Pseudomonas stutzeri XP2, preservation date is on November 27th, 2011, depositary institution is: Chinese Typical Representative culture collection center, preservation address is: China. Wuhan. and Wuhan University, postcode 430072.Be documented in another Chinese patent application of applicant number of patent application 201210201671.5, publication number 102899263A.
Further, described pseudomonas pseudoalcaligenes is selected from the bacterial classification that deposit number is CCTCC NO:M2012225, this bacterium classification called after Pseudomonas pseudoalcaligenes CL-1, preservation date is on June 13rd, 2012, depositary institution is: Chinese Typical Representative culture collection center, preservation address is: China. Wuhan. and Wuhan University, postcode 430072.Be documented in another Chinese patent application of applicant number of patent application 201310032730.5, publication number 103114062A.
Further, described Pseudomonas oleovorans is selected from the bacterial classification that deposit number is CCTCC NO:M2012226, this bacterium classification called after Pseudomonas oleovorans CL-3, preservation date is on June 13rd, 2012, depositary institution is: Chinese Typical Representative culture collection center, preservation address is: China. Wuhan. and Wuhan University, postcode 430072.Be documented in another Chinese patent of applicant the patent No. 201210269891.1, publication number 102776145A.
The preparation method of described mixed bacterial microbial preparation is as follows:
Transfering loop scraping 1 ring lawn for the series bacillus that inclined-plane is preserved, is seeded in 200mL denitrification nutrient solution, and 30～35 ℃, 180～220r/min, 24～36h is cultivated in shaking table concussion, obtains the strain cultured solution of activation; Strain cultured solution is centrifugal 15～20min under 5000～8000r/min, goes supernatant liquor to obtain somatic cells, is diluted to the bacteria suspension of 1.5g wet thallus/L with sterilized water, obtains series bacillus bacteria suspension; Same method (step, parameter, substratum are all identical) prepares Pseudomonas stutzeri bacteria suspension, pseudomonas pseudoalcaligenes bacteria suspension and Pseudomonas oleovorans bacteria suspension; Four kinds of bacteria suspension equal volume amounts are mixed, mix, obtain mixed bacterial microbial preparation.
Consisting of of described denitrification nutrient solution: in every 1L denitrification nutrient solution, contain following component: CH
3cOONa, 2g; KH
2pO
4, 0.4g; MgSO
47H
2o, 0.6g; CaCl
22H
2o, 0.07g; KNO
3, 1g; Tris damping fluid 12mL; Trace element 2mL; Surplus is water; PH value is 7.0～7.2.During preparation, each component is mixed, regulate pH value to 7.0～7.2 (sodium hydroxide solution or hydrochloric acid regulate), 110 ℃ of autoclaving 20min, obtain.
Preferably, described trace element is composed of the following components: EDTA50.0g, ZnSO
42.2g, CaCl
25.5g, MnCl
24H
2o5.06g, FeSO
47H
2o5.0g, (NH
4)
6mo
7o
24H
2o1.1g, CuSO
47H
2o1.57g, CoCl
26H
2o1.61g, distilled water 1000ml, during preparation, mixes each component, obtains.
Described mixed bacterial microbial preparation can be for the treatment of sewage, can strengthen autumn artificial swamp and process the ability of polluted river water and town domestic sewage, by the interaction between filler, plant and microorganism, through processes such as absorption, ion-exchange, plant absorption and microbiological deteriorations, can effectively improve the removal efficiency of pollutent in polluted river water and town domestic sewage.During concrete application, method is: mixed bacterial microbial preparation of the present invention is added in artificial wet land system in autumn (such as Reed Constructed Wetland system in autumn), and add-on is 1% of sewage volume; Treatment time (hydraulic detention time of sewage in drowned flow artificial wet land) is more than 7 days or 7 days.After testing, adopt mixed bacterial microbial preparation of the present invention to dispose of sewage after 7 days, polluted river water water outlet COD after processing, total nitrogen, the content of total phosphorus reaches < < water environment quality standard > > (GB3838-2002) III class water quality standard, town domestic sewage water outlet COD after processing, the content's index of ammonia nitrogen and total phosphorus reaches < < urban wastewater treatment firm pollutant emission standard > > (GB18918-2002) one-level B standard.
Described autumn, the structure of artificial swamp was: comprise matrix, and the high 600～650mm of matrix, diameter 450～500mm, water storage is about 20L.In matrix, apart from 50mm place, matrix bottom, rising pipe is installed, rising pipe is provided with valve and the water outlet for controlling.Water level control pipe is installed, for controlling the water level that is subject to processing sewage in wetland in matrix.Matrix top is provided with water inlet pipe, and water inlet pipe is provided with water-in, for injecting tap water to wetland or being subject to processing sewage.In matrix, be provided with successively from down to up metalling, fine sand layer and pedosphere, wherein, metalling is filled and is formed by the rubble of particle diameter 10～20mm, and thickness is 250～300mm; Fine sand layer is filled and is formed by the fine sand of particle diameter 1～2mm, and thickness is 50-100mm; Pedosphere is filled and is formed by soil, and thickness is 100-150mm.On pedosphere, plant and be implanted with the abundant reed of rhizospheric microorganism.Reed seedling is implanted in artificial wet land system from March kind, every 2 days, with gram nutritive medium (existing conventional products in prior art), water, after one month, start to water tap water (flowing into wet land system by water-in), within every 2 days, change water once, while changing water, open valve, by water outlet, discharged.While disposing of sewage, change and be subject to processing sewage and water.
Described matrix is the right cylinder of PVC material.
The present inventor is through research and practice repeatedly, find: will be by series bacillus (Paenibacillus sp.), Pseudomonas stutzeri (Pseudomonas stutzeri), the mixed bacterial microbial preparation that pseudomonas pseudoalcaligenes (Pseudomonas pseudoalcaligenes) and Pseudomonas oleovorans (Pseudomonas oleovorans) form is added in artificial wet land system, can increase the quantity of rhizosphere denitrogenation flora, the effect of microorganism in strengthening wetland in autumn, can effectively improve the removal efficiency that is subject to processing dirty water pollutant, polluted river water water outlet COD after processing, total nitrogen, the content of total phosphorus can reach < < water environment quality standard > > (GB3838-2002) III class water quality standard, town domestic sewage water outlet COD after processing, the content's index of ammonia nitrogen and total phosphorus can reach < < urban wastewater treatment firm pollutant emission standard > > (GB18918-2002) one-level B standard.
Accompanying drawing explanation
Fig. 1: the present invention is in order to process the small-scale artificial wetland setting drawing of polluted river water and town domestic sewage.
Wherein, 1, water-in; 2, be subject to processing sewage; 3, pedosphere; 4, fine sand layer; 5, gravel bed; 6, reed; 7, observation of stage pipe; 8, valve; 9, water outlet.
Fig. 2: mixed bacterial microbial preparation strengthening Reed Constructed Wetland in autumn is processed the COD design sketch in polluted river water.
Fig. 3: mixed bacterial microbial preparation strengthening Reed Constructed Wetland in autumn is processed the total nitrogen design sketch in polluted river water.
Fig. 4: mixed bacterial microbial preparation strengthening Reed Constructed Wetland in autumn is processed the total phosphorus design sketch in polluted river water.
Fig. 5: mixed bacterial microbial preparation strengthening Reed Constructed Wetland in autumn is processed the COD design sketch of town domestic sewage.
Fig. 6: mixed bacterial microbial preparation strengthening Reed Constructed Wetland in autumn is processed the ammonia nitrogen design sketch in town domestic sewage.
Fig. 7: mixed bacterial microbial preparation strengthening Reed Constructed Wetland in autumn is processed the total phosphorus design sketch in town domestic sewage.
Embodiment
Below in conjunction with embodiment, the present invention is further illustrated.Embodiment is used for illustrating the present invention, rather than limits the present invention.
Embodiment 1 prepares mixed bacterial microbial preparation
Preparation method is as follows:
Transfering loop scraping 1 ring lawn for the series bacillus that inclined-plane is preserved, is seeded in 200mL denitrification nutrient solution, and 30～35 ℃, 180r/min, 24h is cultivated in shaking table concussion, obtains the strain cultured solution of activation; Strain cultured solution is centrifugal 20min under 8000r/min, goes supernatant liquor to obtain somatic cells, is diluted to the bacteria suspension of 1.5g wet thallus/L with sterilized water, obtains series bacillus bacteria suspension; Same method prepares Pseudomonas stutzeri bacteria suspension, pseudomonas pseudoalcaligenes bacteria suspension and Pseudomonas oleovorans bacteria suspension; Four kinds of bacteria suspension equal volume amounts are mixed, mix, obtain mixed bacterial microbial preparation, after testing, in mixed bacterial microbial preparation, class bacillus viable count is 3.6 * 10
8cFU/ml, Pseudomonas stutzeri viable count is 4.2 * 10
8cFU/ml, pseudomonas pseudoalcaligenes viable count is 4.0 * 10
8cFU/ml, Pseudomonas oleovorans viable count is 3.4 * 10
10cFU/ml.
Described series bacillus, deposit number is CCTCC NO:M2011120, Classification And Nomenclature is: Paenibacillussp.XP1, preservation date is on April 10th, 2011, depositary institution is: Chinese Typical Representative culture collection center, preservation address is: China. Wuhan. and Wuhan University, postcode 430072.
Described Pseudomonas stutzeri, deposit number is CCTCC NO:M2011431, Classification And Nomenclature is Pseudomonas stutzeriXP2, preservation date is on November 27th, 2011, depositary institution is: Chinese Typical Representative culture collection center, preservation address is: China. Wuhan. and Wuhan University, postcode 430072.
Described pseudomonas pseudoalcaligenes, deposit number is CCTCC NO:M2012225, Classification And Nomenclature is Pseudomonas pseudoalcaligenes CL-1, preservation date is on June 13rd, 2012, depositary institution is: Chinese Typical Representative culture collection center, preservation address is: China. Wuhan. and Wuhan University, postcode 430072.
Described Pseudomonas oleovorans, deposit number is CCTCC NO:M2012226, Classification And Nomenclature is Pseudomonas oleovorans CL-3, preservation date is on June 13rd, 2012, depositary institution is: Chinese Typical Representative culture collection center, preservation address is: China. Wuhan. and Wuhan University, postcode 430072.
Consisting of of described denitrification nutrient solution: in every 1L denitrification nutrient solution, contain following component: CH
3cOONa, 2g; KH
2pO
4, 0.4g; MgSO
47H
2o, 0.6g; CaCl
22H
2o, 0.07g; KNO
3, 1g; Tris damping fluid 12mL; Trace element 2mL; Surplus is water; PH value is 7.0.During preparation, each component is mixed, regulate pH value to 7.0 (sodium hydroxide solution or hydrochloric acid regulate), 110 ℃ of autoclaving 20min, obtain.
Described trace element is composed of the following components: EDTA50.0g, ZnSO
42.2g, CaCl
25.5g, MnCl
24H
2o5.06g, FeSO
47H
2o5.0g, (NH
4)
6mo
7o
24H
2o1.1g, CuSO
47H
2o1.57g, CoCl
26H
2o1.61g, distilled water 1000ml, during preparation, mixes each component, obtains.
The application of embodiment 2 mixed bacterial microbial preparations in the ability of strengthening artificial swamp processing in autumn polluted river water
As shown in Figure 1, the structure of small-scale artificial wetland is: comprise matrix (for PVC material right cylinder), and high 600～650mm, diameter 450～500mm, water storage is about 20L.In matrix, apart from 50mm place, matrix bottom, rising pipe is installed, rising pipe is provided with valve 8 and the water outlet 9 for controlling.Water level control pipe 7 is installed, for controlling the water level that is subject to processing sewage 2 in wetland in matrix.Matrix top is provided with water inlet pipe, and water inlet pipe is provided with water-in 1, for injecting tap water to wetland or being subject to processing sewage.In matrix, be provided with successively from down to up metalling 5, fine sand layer 4 and pedosphere 3, wherein, metalling 5 is filled and is formed by the rubble of particle diameter 10～20mm, and thickness is 250～300mm; Fine sand layer 4 is filled and is formed by the fine sand of particle diameter 1～2mm, and thickness is 50-100mm; Pedosphere 3 is filled and is formed by soil, and thickness is 100-150mm.On pedosphere 3, plant and be implanted with the abundant reed of rhizospheric microorganism 6.Reed seedling is implanted in artificial wet land system from March kind, every 2 days, with gram nutritive medium (existing conventional products in prior art), water, after one month, start to water tap water (flowing into wet land system by water-in 1), within every 2 days, change water once, while changing water, open valve 8, by water outlet 9, discharged.The experiment starting period is previous month, changes to be subject to processing sewage and to water, and within every 2 days, changes water once, waits for that experiment starts.
Experiment: in autumn (9～November), above-mentioned small-sized reed wetland is carried out to mixed bacterial microbial augmentation and process polluted river water experiment, when ground temperature is at 12～20 ℃.After experiment starts, the mixed bacterial microbial preparation of embodiment 1 preparation is added in artificial swamp in the ratio of 1% (percent by volume), in operational process, average every day from small-sized wetland bottom rising pipe take water sample, sewage stops 7 days in small-scale artificial wetland.Meanwhile, take and do not add mixed bacterial microbial preparation as control group.
Concrete treatment effect is shown in Fig. 2, Fig. 3, Fig. 4.Mixed bacterial strengthening reed wetland is compared with control group, to the removal effect of COD obvious (seeing Fig. 2), add bacterium experimental group in the time of the 7th day, CODcr is 16.2mg/L (≤20mg/L), clearance reaches 75.7%, and water quality reaches < < water environment quality standard > > (GB3838-2002) III class water quality standard (CODcr≤20mg/L).Control group CODcr is still below standard in the time of the 7th day.Fig. 3 demonstration, in experimentation, reed adds TN concentration in bacterium strengthening wetland and presents significantly reduced trend, compares have more excellent removal effect with control group.In the time of the 7th day, TN concentration is 0.6mg/L (≤1.0mg/L), clearance reaches 96.65%, reaches < < water environment quality standard > > (GB3838-2002) III class water quality standard (TN≤1.0mg/L).And the clearance of control group is only 38.5%.Adding of mixed bacterial, to reed wetland strengthening in autumn town domestic sewage, significant effect has been played in denitrogenation.Fig. 4 demonstration, adds bacterium group and compares with control group, and TP concentration significantly reduces.In the time of the 7th day, the TN concentration that adds bacterium group is 0.15mg/L (<0.2mg/L), clearance reaches 90.4%, reach < < water environment quality standard > > (GB3838-2002) III class water quality standard (TP≤0.2mg/L), the removal of reed wetland to TP in polluted river water strengthened in adding of mixed bacterial.
The application of embodiment 3 mixed bacterial microbial preparations in the ability of strengthening artificial swamp processing in autumn town domestic sewage
Autumn (9～November) small-sized reed wetland (in embodiment 2) is carried out to mixed bacterial microbial augmentation and process town domestic sewage experiment, when ground temperature is at 12～20 ℃.After experiment starts, the mixed bacterial microbial preparation of activation is added in artificial swamp in 1% ratio, in operational process, average every day from each small-sized wetland bottom rising pipe take water sample, sewage stops 7 days in small-scale artificial wetland.Meanwhile, take and do not add mixed bacterial microbial preparation as control group.
Concrete treatment effect is shown in Fig. 5, Fig. 6, Fig. 7.Mixed bacterial strengthening reed wetland is compared with control group, to the removal effect of COD obvious (seeing Fig. 5), add bacterium experimental group in the time of the 4th day, CODcr is 55mg/L (≤60mg/L), clearance reaches 67.6%, and water quality reaches < < urban wastewater treatment firm pollutant emission standard > > (GB18918-2002) one-level B standard (CODcr≤60mg/L).In the time of the 7th day, adding bacterium group clearance is 85.3%, control group clearance 63.5%.Control group CODcr is up to standard in the time of the 7th day.Fig. 6 demonstration, in experimentation, reed adds NH in bacterium strengthening wetland
3-N concentration presents the trend reducing gradually, compares have more excellent removal effect with control group.NH in the time of the 7th day
3-N concentration is down to 6.2mg/L (≤8mg/L), clearance reaches 87.4%, and water quality reaches < < urban wastewater treatment firm pollutant emission standard > > (GB18918-2002) one-level B standard (NH
3-N≤8mg/L).And control group clearance is 52.64%, below standard.Fig. 7 shows, adding bacterium strengthening experimental group compares with control group, TP concentration significantly reduces, in the time of the 7th day, TN concentration is 0.4mg/L (<0.5mg/L), clearance reaches 88.9%, and water quality reaches < < urban wastewater treatment firm pollutant emission standard > > (GB18918-2002) one-level B standard-required (TP≤0.5mg/L).And the TP clearance of control group is 65%, below standard.
Comprehensive embodiment 2,3, shows, the device of applying in the present invention is simple to operate, in operational process, without aeration, takes full advantage of the good action of microorganism, can reach emission standard, can effectively save process operation cost.By add mixed bacterial to autumn artificial swamp carry out biological reinforced, hydraulic detention time is in the time of 7 days, and COD, the N, the P content's index that are subject to processing sewage reach < < water environment quality standard > > (GB3838-2002) III class water quality standard or < < urban wastewater treatment firm pollutant emission standard > > (GB18918-2002) one-level B standard.
Claims (8)
1. a mixed bacterial microbial preparation, is characterized in that: the bacteria suspension being comprised of series bacillus, Pseudomonas stutzeri, pseudomonas pseudoalcaligenes and Pseudomonas oleovorans, wherein, viable count is 3.8 * 10
8more than CFU/ml.
2. mixed bacterial microbial preparation according to claim 1, is characterized in that:
Described series bacillus is selected from the bacterial classification that deposit number is CCTCC NO:M2011120, this bacterium classification called after: Paenibacillus sp.XP1, preservation date is on April 10th, 2011, depositary institution is: Chinese Typical Representative culture collection center, preservation address is: China. Wuhan. and Wuhan University, postcode 430072;
Described Pseudomonas stutzeri is selected from the bacterial classification that deposit number is CCTCC NO:M2011431, this bacterium classification called after Pseudomonas stutzeri XP2, preservation date is on November 27th, 2011, depositary institution is: Chinese Typical Representative culture collection center, preservation address is: China. Wuhan. and Wuhan University, postcode 430072;
Described pseudomonas pseudoalcaligenes is selected from the bacterial classification that deposit number is CCTCC NO:M2012225, this bacterium classification called after Pseudomonas pseudoalcaligenes CL-1, preservation date is on June 13rd, 2012, depositary institution is: Chinese Typical Representative culture collection center, preservation address is: China. Wuhan. and Wuhan University, postcode 430072;
Described Pseudomonas oleovorans is selected from the bacterial classification that deposit number is CCTCC NO:M2012226, this bacterium classification called after Pseudomonas oleovorans CL-3, preservation date is on June 13rd, 2012, depositary institution is: Chinese Typical Representative culture collection center, preservation address is: China. Wuhan. and Wuhan University, postcode 430072.
3. the preparation method of the mixed bacterial microbial preparation described in claim 1 or 2, it is characterized in that: transfering loop scraping 1 ring lawn for the series bacillus that inclined-plane is preserved, be seeded in 200mL denitrification nutrient solution, 30～35 ℃, 180～220r/min, 24～36h is cultivated in shaking table concussion, obtains the strain cultured solution of activation; Strain cultured solution is centrifugal 15～20min under 5000～8000r/min, goes supernatant liquor to obtain somatic cells, is diluted to the bacteria suspension of 1.5g wet thallus/L with sterilized water, obtains series bacillus bacteria suspension; Same method prepares Pseudomonas stutzeri bacteria suspension, pseudomonas pseudoalcaligenes bacteria suspension and Pseudomonas oleovorans bacteria suspension; Four kinds of bacteria suspension equal volume amounts are mixed, mix, obtain mixed bacterial microbial preparation;
Consisting of of described denitrification nutrient solution: in every 1L denitrification nutrient solution, contain following component: CH
3cOONa, 2g; KH
2pO
4, 0.4g; MgSO
47H
2o, 0.6g; CaCl
22H
2o, 0.07g; KNO
3, 1g; Tris damping fluid 12mL; Trace element 2mL; Surplus is water; PH value is 7.0～7.2.During preparation, each component is mixed, regulate pH value to 7.0～7.2,110 ℃ of autoclaving 20min, obtain.
4. the application of the mixed bacterial microbial preparation described in claim 1 or 2 in disposing of sewage.
5. the application of the mixed bacterial microbial preparation described in claim 1 or 2 in the ability of reinforcement artificial swamp in autumn processing polluted river water and town domestic sewage.
6. according to the application described in claim 4 or 5, it is characterized in that: while specifically applying, method is: mixed bacterial microbial preparation is added in artificial wet land system in autumn, and add-on is 1% of sewage volume; Treatment time is more than 7 days or 7 days.
7. application according to claim 6, is characterized in that: described autumn, the structure of artificial swamp was: comprise matrix, the high 600～650mm of matrix, diameter 450～500mm; In matrix, apart from 50mm place, matrix bottom, rising pipe is installed, rising pipe is provided with valve and the water outlet for controlling; Water level control pipe is installed in matrix; Matrix top is provided with water inlet pipe, and water inlet pipe is provided with water-in; In matrix, be provided with successively from down to up metalling, fine sand layer and pedosphere, wherein, metalling is filled and is formed by the rubble of particle diameter 10～20mm, and thickness is 250～300mm; Fine sand layer is filled and is formed by the fine sand of particle diameter 1～2mm, and thickness is 50-100mm; Pedosphere is filled and is formed by soil, and thickness is 100-150mm; On pedosphere, plant and be implanted with reed.
8. application according to claim 7, is characterized in that: reed seedling is implanted in artificial wet land system from March kind, every 2 days, with gram nutritive medium, waters, and after one month, starts to water and pours water, and within every 2 days, changes water once; While disposing of sewage, change and be subject to processing sewage and water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410181469.XA CN103923868B (en) | 2014-04-30 | 2014-04-30 | Mixed bacterial microbial preparation and the application in disposing of sewage thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410181469.XA CN103923868B (en) | 2014-04-30 | 2014-04-30 | Mixed bacterial microbial preparation and the application in disposing of sewage thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103923868A true CN103923868A (en) | 2014-07-16 |
| CN103923868B CN103923868B (en) | 2016-04-06 |
Family
ID=51142267
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410181469.XA Active CN103923868B (en) | 2014-04-30 | 2014-04-30 | Mixed bacterial microbial preparation and the application in disposing of sewage thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103923868B (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105347481A (en) * | 2015-11-26 | 2016-02-24 | 山东大学 | Method for purifying low-carbon-nitrogen-ratio domestic sewage by biological fermentation coupling artificial wetland system |
| CN105347514A (en) * | 2015-12-10 | 2016-02-24 | 郭嘉川 | Sewage treatment accelerant and preparation method thereof |
| CN105481102A (en) * | 2015-12-30 | 2016-04-13 | 中林山水（北京）生态科技股份有限公司 | Upgrading modification method for improving water quality of sewage effluent through microorganism purification technology |
| CN106676021A (en) * | 2015-11-06 | 2017-05-17 | 丹阳市尚德生物科技有限公司 | Microbial preparation for river course water quality restoration, and preparation method thereof |
| CN109105237A (en) * | 2018-06-26 | 2019-01-01 | 南京工程学院 | Remove and measure the experimental method that wetland plant rhizosphere microorganism group influences phosphorus in plant absorption water |
| WO2019041792A1 (en) * | 2017-08-29 | 2019-03-07 | 暨南大学 | Method and system for enhancing artificial wetland for removal of microcystis and toxins in water |
| CN109576189A (en) * | 2018-12-29 | 2019-04-05 | 合肥市东方美捷分子材料技术有限公司 | A kind of black and odorous water composite bacteria agent and its preparation method and application |
| CN110241050A (en) * | 2019-07-04 | 2019-09-17 | 江苏宜裕环保科技有限公司 | A kind of compound Autotrophic nitrification bacterium, the preparation method of its solid fungicide and the application in the sewage treatment containing high salt and high ammonia nitrogen |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1566642A (en) * | 2003-06-26 | 2005-01-19 | 乐金电子(天津)电器有限公司 | Frame of hermetic compressor and its manufacturing method |
| CN101941762A (en) * | 2010-09-08 | 2011-01-12 | 福建微水环保技术有限公司 | Method for treating wastewater by utilizing composite microbial preparation |
| KR20120027979A (en) * | 2010-09-14 | 2012-03-22 | 바이오밴코리아(주) | The microbial seeds which having excellent wastewater treatment ability and producing method for the sames and the wastewater treatment method |
| WO2012079140A1 (en) * | 2010-12-14 | 2012-06-21 | Gustavo Casal De Rey | Biostimulation and bioenhancing processes for treating domestic and industrial effluents |
| CN102583775A (en) * | 2012-02-14 | 2012-07-18 | 福建微水环保技术有限公司 | Composite microorganism preparation and application thereof in waste water treatment |
-
2014
- 2014-04-30 CN CN201410181469.XA patent/CN103923868B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1566642A (en) * | 2003-06-26 | 2005-01-19 | 乐金电子(天津)电器有限公司 | Frame of hermetic compressor and its manufacturing method |
| CN101941762A (en) * | 2010-09-08 | 2011-01-12 | 福建微水环保技术有限公司 | Method for treating wastewater by utilizing composite microbial preparation |
| KR20120027979A (en) * | 2010-09-14 | 2012-03-22 | 바이오밴코리아(주) | The microbial seeds which having excellent wastewater treatment ability and producing method for the sames and the wastewater treatment method |
| WO2012079140A1 (en) * | 2010-12-14 | 2012-06-21 | Gustavo Casal De Rey | Biostimulation and bioenhancing processes for treating domestic and industrial effluents |
| CN102583775A (en) * | 2012-02-14 | 2012-07-18 | 福建微水环保技术有限公司 | Composite microorganism preparation and application thereof in waste water treatment |
Non-Patent Citations (2)
| Title |
|---|
| 吉芳英等: "污水处理中微生物反硝化脱氮过程及代谢规律", 《四川理工学院学报(自然科学版)》, vol. 26, no. 4, 31 August 2013 (2013-08-31) * |
| 王国惠等: "生活污水高效降解混合菌群的研究", 《给水排水》, vol. 31, no. 5, 31 December 2005 (2005-12-31) * |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106676021A (en) * | 2015-11-06 | 2017-05-17 | 丹阳市尚德生物科技有限公司 | Microbial preparation for river course water quality restoration, and preparation method thereof |
| CN105347481A (en) * | 2015-11-26 | 2016-02-24 | 山东大学 | Method for purifying low-carbon-nitrogen-ratio domestic sewage by biological fermentation coupling artificial wetland system |
| CN105347481B (en) * | 2015-11-26 | 2017-11-03 | 山东大学 | The method that biofermentation coupling artificial wet land system purifies low ratio of carbon to ammonium sanitary sewage |
| CN105347514A (en) * | 2015-12-10 | 2016-02-24 | 郭嘉川 | Sewage treatment accelerant and preparation method thereof |
| CN105481102A (en) * | 2015-12-30 | 2016-04-13 | 中林山水（北京）生态科技股份有限公司 | Upgrading modification method for improving water quality of sewage effluent through microorganism purification technology |
| CN105481102B (en) * | 2015-12-30 | 2018-03-02 | 中林山水（北京）生态科技股份有限公司 | A kind of remodeling method of promoting to a higher rank with microbial cleaning skill upgrading sewage effluents water quality |
| WO2019041792A1 (en) * | 2017-08-29 | 2019-03-07 | 暨南大学 | Method and system for enhancing artificial wetland for removal of microcystis and toxins in water |
| CN109105237A (en) * | 2018-06-26 | 2019-01-01 | 南京工程学院 | Remove and measure the experimental method that wetland plant rhizosphere microorganism group influences phosphorus in plant absorption water |
| CN109576189A (en) * | 2018-12-29 | 2019-04-05 | 合肥市东方美捷分子材料技术有限公司 | A kind of black and odorous water composite bacteria agent and its preparation method and application |
| CN110241050A (en) * | 2019-07-04 | 2019-09-17 | 江苏宜裕环保科技有限公司 | A kind of compound Autotrophic nitrification bacterium, the preparation method of its solid fungicide and the application in the sewage treatment containing high salt and high ammonia nitrogen |
| CN110241050B (en) * | 2019-07-04 | 2021-05-28 | 江苏宜裕环保科技有限公司 | Compound autotrophic nitrifying bacteria, preparation method of solid microbial inoculum thereof and application of compound autotrophic nitrifying bacteria in sewage treatment containing high salinity and high ammonia nitrogen |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103923868B (en) | 2016-04-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103923868B (en) | Mixed bacterial microbial preparation and the application in disposing of sewage thereof | |
| Lu et al. | Study on method of domestic wastewater treatment through new-type multi-layer artificial wetland | |
| Abdel-Raouf et al. | Microalgae and wastewater treatment | |
| Shao et al. | Bioaugmentation in lab scale constructed wetland microcosms for treating polluted river water and domestic wastewater in northern China | |
| CN101224940A (en) | River sediment in-situ remediation method | |
| CN102464404B (en) | Method and system for remediation and treatment of polluted river water | |
| CN103922536B (en) | New method for comprehensive treatment of black and odorous river | |
| CN110240286B (en) | Black and odorous water body treatment method based on biochar immobilized composite microorganism treatment and aeration reoxygenation system thereof | |
| CN105502867A (en) | Method for treating black and odorous water body bottom mud through microelectrolysis material | |
| CN104232546A (en) | Construction method and application of immobilized biological bacterium agent for micro-polluted water source | |
| CN108070543A (en) | It is a kind of to be suitable for preparations and application of the low C/N than the denitrogenation bacteria preparation of wastewater treatment | |
| CN106115904A (en) | A kind of device and method utilizing microorganism in situ to excite quickly process black and odorous water | |
| CN102897980A (en) | Combined town sewage treatment constructed wetland system and town sewage treatment method | |
| CN102776140B (en) | Cold-tolerant pseudomonas strain Den-05, and screening method and application thereof | |
| Zhao et al. | Ecological floating bed for decontamination of eutrophic water bodies: Using alum sludge ceramsite | |
| Ni et al. | Demonstration research project of a new three-stage bio-oxidation pond for purifying black smelly water bodies | |
| CN204737846U (en) | Handle agriculture non -point source pollution's perpendicular constructed wetland system of upwelling | |
| CN111777268A (en) | Ecological purification method of landscape water body based on modified plant tannin | |
| CN104973738B (en) | Method for removing pollutants from chromium-containing sewage | |
| Upadhyay et al. | Sustainable phytoremediation strategies for river water rejuvenation | |
| CN202968310U (en) | Combined type artificial wetland system for municipal wastewater treatment | |
| Peng et al. | Biofilter-constructed wetland-trophic pond system: A new strategy for effective sewage treatment and agricultural irrigation in rural area | |
| Mulligan et al. | Innovative biological treatment processes for wastewater in Canada | |
| Biswas et al. | Nature-inspired ecotechnological approaches toward recycling and recovery of resources from wastewater | |
| CN108821449A (en) | A kind of method and system suitable for domestic sewage in rural areas advanced treating |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |