WO2012079140A1

WO2012079140A1 - Biostimulation and bioenhancing processes for treating domestic and industrial effluents

Info

Publication number: WO2012079140A1
Application number: PCT/BR2011/000474
Authority: WO
Inventors: Gustavo Casal De Rey
Original assignee: Gustavo Casal De Rey
Priority date: 2010-12-14
Filing date: 2011-12-14
Publication date: 2012-06-21
Also published as: BRPI1005456A2

Abstract

The present patent application relates to processes that include the use and application of specific micro-organisms in various types of effluents. The use of the process for treating various types of effluents, both domestic and industrial, with various nutrient sources and the necessary adaptation of a biological composition comprising micro-organisms (and nutrients for these micro-organisms), aim at improving the capacity to remove undesirable pollutants during effluent treatment processes.

Description

"BIO-STIMULATION AND BIO-INCREASING PROCESSES FOR TREATMENT OF DOMESTIC AND INDUSTRIAL WASTE"

FIELD OF INVENTION

The present patent application relates to processes comprising the use and application of microorganisms based on a nutritional and microbiological process, such microorganisms being specific for application in various types of effluents.

SUMMARY OF THE INVENTION

The application of the process in the treatment of various types of effluents, whether domestic or industrial, with various nutritional sources and the need to adapt a biological composition, which comprises microorganisms (and nutrients for these) vision to improve the removal capacity of non-polluting elements. desired in wastewater treatment processes.

The process aims at the treatment of domestic effluents and those from various industry segments, such as dairy, cellulose, textile, pulp and paper, oil and other segments, through biological complementation (microorganisms and nutrients), according to with the effluent specificity, for a more adequate and efficient yield of its processes.

BRIEF DESCRIPTION OF THE FIGURE

Figure 1 represents the Monod curve for the growth of microorganisms in pure cultures.

BACKGROUND OF THE INVENTION

The treatment of pollution, whether air, water or soil, aims at reducing the concentration levels of polluting elements in order to obtain a post-treatment result with acceptable levels for release into the environment, aiming to eliminate and minimize the negative environmental impact.

Pollution control consists of identifying their source (s), measuring pollutant elements, determining the most appropriate type of treatment, and checking the levels of pollutant elements after treatment.

Effluents from different types of industries may contain compounds of different natures and there are different ways to treat them, aiming to make the effluent in an acceptable state for discharge into the receiving body.

Effluent treatment processes can be categorized into primary (physical, chemical), secondary (biological, physical / chemical), tertiary (biological, physical / chemical), and each phase increases the levels of complexity and resources. for their implementation, as assessed by existing pollutants.

In the physicochemical process, there is inclusion of polymers, mostly coagulant and other flocculant, which may be anionic or cationic, depending on the source of pollution (industry segment). Prior evaluation of the effluent is necessary to properly adjust and obtain greater control of said process. One way to perform the test would be to use the "JAR-Test" to determine the optimal dosages of coagulants and other chemicals involved in the process.

Prior effluent assessment is required to properly adjust the control over the treatment. JAR-Test makes it possible to evaluate the products with the best efficiency. This procedure is repeated in the field, maintaining the product application scale (Ex: 1 liter in the evaluation for 1000 liters of the effluent system).

Already in the biological process (anaerobic or aerobic), despite advances in the field of sanitary engineering, there is still a need to control the process, due to field variations that can generate instability. The search for correction of this process can lead to significant loss of "time", consequently with loss of quality of the final effluents. This can be because the biological correction process is slower and the systems generally do not have storage tanks or safety systems (also called "safeguard") which results in the release of effluents with higher levels of pollutants in the environment. environment. This fact occurs while the biological reactor instability lasts, which is responsible for much of the pollutant removal.

Due to the great diversity of industrial activities, there is a wide variety of components that may be present in the effluents, so there is a need to adapt the biological system in order to strengthen and diversify the pre-existing microbiota in its biological reactors.

Regarding the operational and quality aspects of the treatment plants, the process involves the maintenance of operations in order to keep the biological population stable for sludge control. The sludge constitution comprises the total solids divided into: fixed solids, suspended solids and dissolved solids. Through this measure, we represent the organic matter, nutrients and pathogenic microorganisms or not present in the treatment system, in this case the biological reactor.

Sludge control is associated with the BOD / SSV ratio, which indicates the conversion of BOD (Biochemical Oxygen Demand - Reference Measurement of the Effluent Pollutant Load, to volatile suspended solids, ie organic substrates and microorganisms, increasing solids The treatment process transforms the effluent into solids, releasing water without soluble BOD, making its pollutant load much smaller and making the effluent after the solids removal makes the water treated.

With the high conversion rate in most systems (0.5 BOD / SSV), ie every 1 kg of BOD, there is transformation in 0.5 kg of SSV in the biological reactor (aerated system), which must be periodically removed, which involves considerable costs depending on the system, especially those that handle large BOD loads (city stations, large water consuming companies in their processes), having the most efficient aerobic systems, but are the ones that generate the most solids.

The composition of the sludge is almost entirely biodegradable, representing about 98% of the solids present, as it is basically composed of new microorganisms, pre-consumed substrates, dead microorganisms, pre-digested substrates, and unrefrigerated substrates. -consumed and minerals.

Currently for wastewater treatment biological replacement that involves the application of products that, among the microbiota present in them, include: Bacillus subtillis, Pseudomonas stutzeri, Bacillus sp, Escherichia hermanii, Escherichia lincheniformis, Bacillus amyloliquefaceas,

Paenibacillus polymyxa, Pseudomonas fluorescens, and Pseudomonas putida, and other miscellaneous microorganisms occurring in the process.

The treatment of effluents that have a high content of fatty oils, it is common the use of products that comprise microogranisms such as Agrobacterium radiobacter, Azospirillum lipoferum, Bacillus licheniformis; protozoa such as Aspidisca, Vorticella; rotifers like Philodina and Epiphanes.

For the control and reduction of sludge, microorganisms selected from: filamentous bacteria, Beggiatoa Alba, Bacillus licheniformis, Rhodospirillum rubrum, Aquaspirillum gracille, rotifers: Brachionus, Keratella and protozoa: Paramecium, Lionotus.

To assist the degradation of oils, greases and organic chemical compounds, microorganisms selected from the bacteria are used: Agrobacterium radiobacter, Azospirillum lipoferum, Bacillus licheniformis; protozoa: Aspidisca, Vorticella and rotifers: Philodina, Epiphanes.

Through this process and the induction of these microbiological families, which adapt significantly to a wide range of organic compounds as their energy source, makes more organic compounds measured in BOD to be consumed in order to improve biological digestion, and thus stabilizing the biological process and reducing the BOD / SSV ratio to very low levels, which makes the system more efficient in consuming organic loads and converting them to gases. Thus we have a more balanced effluent treatment system, and with less generation of "waste" / sludge.

Activated Sludge Microbiology

In the biological effluent treatment process, an artificial reproduction of the biodegradation mechanism that occurs in rivers is performed. One of the most commonly used processes is activated sludge which refers to the continuous aerobic fermentation process with recycling (return of sludge separated from treated effluent) from biomass which constitutes a permanent and acclimatized inoculum.

From the nutritional and energetic point of view, living beings are classified into two large groups, autotrophic beings, which have the ability to fix carbon dioxide through photosynthesis or chemosynthesis. Heterotrophic beings, on the other hand, require organic substrates to remove their energy needed for their development.

However this classification is very simple and insufficient to explain the variety of nutritional pathways that organisms use. Thus, based on two important parameters, the nature of the energy source and the main carbon source, it is possible to group the microorganisms into four very distinct categories: photoautotrophic, photoheterotrophic, chemoautotrophic, chemoheterotrophic.

The growth of microorganisms in pure cultures can be observed according to the growth curve model described by Monod, as shown in figure 1. A culture in a closed system has the growth of the described microorganisms according to the Monod curve model (FIGURE 1), which comprises the steps described below.

1 - Lag phase or acclimatization - there is no increase of microorganisms, and in this stage they prepare the enzymatic arsenal, necessary for the consumption of substrates.

2 - Acceleration phase - microbial growth begins

3- Log or exponential phase - given the proper living conditions (abundant substrates and low toxins), microorganisms grow at maximum speed, represented by an exponential function.

4 - Deceleration phase: the growth that was previously maximum, will now begin to decay, due to the decrease of substrates, and the accumulation of toxic excreta.

5 - Stationary phase - growth velocity becomes zero, due to substrate depletion and toxicity levels are already incompatible with microbial development.

6 - Decline or endogenous phase - there is a decrease in the number of microorganisms caused by death and lysis.

The mixed bacterial population does not remain in synchronized growth while one part is in the exponential phase, another may be in the stationary phase and / or other phases. Due to such particularities in bacterial growth, process control is required to maintain maximum possible in steady and declining phases, ie endogenous phase.

The endogenous phase of treatment is important, since there is a decrease in biomass due to auto-oxidation (endogenous metabolism) and also because in this phase bacterial flocculation occurs. Flocculation of the sludge is an important step in the process because it allows the formed bacterial mass to be separated from the treated effluent and returned to the system, making the sludge activated by this concentration (Vazoller, 1989).

Microorganisms in activated sludge treatment process

In activated sludge systems, the organisms present are not necessarily the same as those of natural freshwater environments. This is because the process has specific characteristics, such as turbulence, due to aeration, in the process of oxygen induction in water, carried out by aerators (equipment used to transfer oxygen and which allows accelerating cellular metabolism), typical of the process, such as turbidity. due to suspended material in the reactor.

Only microfauna are found in these environments, as turbulence does not allow the development of larger organisms. The turbidity of the medium causes the absence of light, preventing the development of algae. Among the microbiota are usually found various types of bacteria, including the filaments that form biomass, protozoa, micro metazoans, and eventually fungi and yeast. Bacteria are saprobic organisms, primary consumers that degrade the organic load of the effluent, promoting its stabilization. organic charge had been converted to total solids.

The filamentous bacteria, present in both flakes and free, have equal degradation capacity of organic matter, but should be controlled to avoid problems in sludge decantation, preventing it from settling, as they can separate the sludge from the treated effluent and from it. way decreases the quality of the final effluent.

The presence of micro-fauna is an important indicator of the effluent treatment process functioning, being used as a biological indicator. The identification of bacteria is a generally slower and more expensive process compared to protozoa, which makes their use as indicators difficult. In treatment plants there is a preference for simpler and more efficient methods such as solids concentration measurement, especially volatile suspended solids.

The qualitative and quantitative microbiota analysis is performed to control the activated sludge degradation processes, however there is still a lack of diagnostic tools and problems of sampling, counting and data utilization due to the complexity of the interactions.

Bacterial populations (primary decomposers) fix a complex substrate, usually variable in quality and quantity. From these decomposing organisms lives a fauna of primary consumers, also subject to predation among themselves (protozoa). Interactions, both competition and predation, are very diverse.

Bacterial flakes should be limited as regards refer to the volume and concentration, where they are controlled by the series of solids, to a point where there are no problems in sludge decantation and filamentous sludge swelling due to the large presence of filamentous bacteria. Improper sludge decantation may have other origins such as an increase in a type of bacteria such as Zooglea ramigera or filamentous fungi, which also diminishes the quality of the final effluent.

The appearance of the sludge under the microscope can generally be described as follows: Bacteria aggregate into biological flakes, which also congregate filamentous bacteria. On the surface of these flakes the sessile, pedunculated ciliary protozoa or peritricias are fixed. There are protozoa that live in close connection with the flakes, feeding on them and always remaining around them, but not physically attached to them (ciliated hypotrichies). Finally, there are the free-floating ciliates, which move in the spaces between the flakes, the flagellates and the amoebas, the latter two being preferably on the surface of the flake, as in the space between them, depending on the species. Micro metazoans (rotators and small worms) also generally move in the space between the flakes.

Precise determination of all species present is difficult to perform in a control work. Thus, simplified counts of micro-fauna arranged in classes or average residence time of the sludge in the reactor are used. Saprobicity characteristics, water quality level reflected by the species constituting the present community, according to the biodegradable organic matter expressed in BOD (Biochemical Oxygen Demand), are also used. which represents the analytical result of the amount of oxygen required to biologically oxidize a certain amount of organic matter over five days incubated at 20 ° C.

In aerated sludge treatments, such as the activated sludge process, the medium in the aeration tank, depending on saprobicity, may vary from oligosabrobics (excellent scrubbing conditions, with DB05 (average torso 2.5 mg / l ), polisaprobic (lower clearance conditions, with DB05 average around 50 mg / l) The intermediate effluent quality level conditions that may be present are: β-mesosaprobic (BOD average of 5 mg / l) and α-mesosaprobics (BOD average around 10 mg / l) Conditions and α-mesosaprobics are the most frequent in aerated dump treatments.

Microbial species react to medium selection factors (trophic and physicochemical) individually according to their own characteristics. The fact that micro-fauna undergoes the simultaneous action of all process parameters and subsists in non-ideal conditions makes it a very sensitive indicator. The micro-fauna is therefore an indicator of the set of activated sludge operating parameters, since its nature varies with the level of purification, the dissolved oxygen concentration, the presence of toxic substances etc, inside the tank. aeration (Vazoller, 1989)

MICROBIOTA

Among the variety of microorganisms below is a description of those commonly found in effluents and sludge.

Bacteria

In addition to the Zoogloea ramiera, considered by many As the sole responsible for flocculation, there are those belonging to the genera achromobacterium, chromobacterium (flavobacterium) and pseudomonas, especially gram-negative rods with proteolytic action.

Zoogloea has the ability to form gelatinous masses, identified under the microscope due to the formation of dentitic structures. Among the filamentous bacteria, Sphaerotilus natans is the most common in activated sludge, characterized by sheath and false branching. They are thin filaments and, in general, the cellular septa are not visible. There are other bacteria that may be present in the process such as Thiotrix, Beggiatoa and Nocardia among others.

Overgrowth of filamentous bacteria makes sludge decantation difficult, causing a condition called filamentous sludge swelling. Therefore, constant control of filament concentration is required, otherwise it may lead to loss of solids by the effluent as it does not sediment and is "dragged" along with the final effluent, losing its quality.

Fungi

Fungi are not very common in activated sludge, and when present, are usually deuteromycetes (imperfect fungi) of the most commonly encountered species of the genus Geotrichum. When they overdevelop, they can also cause the sludge to swell. They may predominate in processes where there is a sharp drop in pH.

The frequently encountered microbiota can be grouped according to the table below: Table 01: Grouping of organisms of different genera that are most frequent.

Source: Vazoller, 1989

In the table below, information regarding the microbial analysis of active sludge that operates with domestic sewage.

Table 2: Typical microbial analysis for an activated sludge operating with domestic sewage

Group Order of magnitude org / mL

Free Ciliates IO ³ to IO ⁴

Pedunculate Ciliates 10 ³ to 10 ⁴

Rhizopods IO ² to 10 ⁴

Flagged IO ² to IO ³

Rotifers IO ² to IO ³

Nematodes ¹ to 10 IO ²

Annelida ¹ to 10 IO ² The products currently used present satisfactory results with good applicability in various treatment processes and systems. Despite the good efficiency, the high cost of the large scale process makes the process costly to obtain promising results and financial gains, reducing the operating costs of the wastewater treatment system, especially its waste generation, sludge. , given that with the increase and biological diversification of the process, reducing the operational variations and mainly the environmental liability of the pollution control system, consuming the existing substrates, the cellular decay, making the sludge much more active, without "interference". , improving quality and mainly reducing the BOD / SSV (Biochemical Oxygen Demand / Volatile Soluble Solids) ratio, to lower values and tending the stability of the sludge concentration in the biological reactors, which makes them more operationally reliable, with lower costs and creating the possibility of maximum performance of the technical design of the pollution control system tion - treatment plant and liquid effluent.

PI 0516578 claims a bacterial composition comprising the bacteria Nitrosomas eutropha and AJitrojacter winogradskyi for the purpose of removing ammonia and nitrite.

PI 0704520 relates to the removal of phenolic compounds from wastewater from the pulp and paper industry, the rubber, glues, adhesives, impregnating resins, electrical components, plastics and steel industry by the use of the fungus Pleurotus ssp.

Document PI0802681 claims a mixture which It comprises nutrients such as nitrogen and phosphorus, with the purpose of treating effluents from the pulp and paper industry and biological treatments. The subject matter has restrictions as it applies only to effluents from the mentioned industries. The mixture is related to the biostimulation process, the present patent application claims a method and method for treating various types of effluents comprising biostimulation and biostimulation.

PI 0519018 claims an effluent treatment process comprising the use of pathogenic microorganisms or not. The process comprises pH control between 6 and 10 and introduction of electrodes for direct current application. The process is distinct from the present patent application comprises the biostimulation and biostimulation processes.

PI 0802601 refers to a process of detoxification and effluent treatment with water recycling and utilization of organic and inorganic fillers. The process is intended for the treatment of vinasse, effluent from the ethanol producing industry that consists of the combined application of chemical, physical-chemical, photochemical and biotechnological methods. The process involves oxidation via ozonation, application of adsorbents. Such processes are distinct from the present patent application.

The present invention relates to a process of biostimulation and biostimulation and biological composition, for application in the treatment of effluents from different industrial and / or domestic processes, and which enable the consumption of organic nutrients to be optimized resulting in a reduction of costs and better maintenance of environmental pollution control systems at industrial and domestic wastewater treatment plants.

The process of biostimulation consists of the adaptation of the environment by the addition of nutrients to stimulate bacteria or other microorganisms capable of biodegradation of the organic compounds (pollution / BOD) in the effluents.

The bio-augmentation process consists of the "multiplication" and addition of microorganisms, in a certain "environment" (biological reactor), aiming to promote the increase in the degradation process of the present compounds.

The biostimulation process potentiated by the bioaccumulation process, but in order to be effective, it is necessary that the components be those essential for the present microorganisms. Added to this is the need to promote a synergistic effect among the microorganisms of the environment without competition and elimination of communities that may impair the process of treatment of the environment of interest.

The diversification of the microorganisms present in the effluent to be treated, coupled with the addition of nutrients, enables the increase in the consumption of organic compounds in order to improve the effluent quality, besides saving time and cost. It should be considered that in certain situations, the present microorganisms are not able to degrade the added matter, whatever the origin, so under these conditions the process becomes more important in order to acclimate the relevant biological cultures. DETAILED DESCRIPTION OF THIS INVENTION

The process is based on natural concepts, through a wide-ranging nutritional medium, to meet a large and varied species of microorganisms, with different microbiological nutritional needs, using chemical-organic compound for use in the technique of biostimulation. and after the desired product, the technique of bioaccumulation of the desired naturally occurring microorganisms is applied, and registered under CONAMA 314 (Bioaccumulation Program an advanced technology for the treatment of dairy effluents. Technical material referred), and in the Ministry of agriculture.

The present technology relates to a wastewater treatment process which has a low cost for the applied volume of the product in the treatment system, comprising the steps described below.

Phase 1 corresponds to the preparation of the biochemical compound (compound to be used in the biostimulation phase) which consists of a composition comprising various nutrients such as simple broth (culture medium dating from 1880).

The process consists of the application of the compound for the treatment of effluents. The manner in which the composition is made available provides significant operating gains due to changes in the volume of the product to be applied beyond the economic ones. Comparatively, there are gains in the application of 1000 liters of the biostimulated and biostimulated product of the present invention as compared to lOKg of products on the market. The advantages of having a large volume refer to the best conditions to encourage the largest volume of effluent. possible due to the possibility of dosing in smaller and constant fractions (possibility of one dosage per second, depending on the dosing system) and easier handling by the operator, unlike application with solid composition.

After mixing, the two-phase compound is added to amplify the biological activity to improve yield and thereby promote the removal of the desired polluting compounds.

EXAMPLES

The examples described in this patent application are intended to indicate some of the possible variations of embodiment of the developed technology and are not restricted to such.

For the execution of the effluent treatment process from the most varied production processes, there are some steps that must be performed and such specifications provide technical advances in relation to the state of the art.

EXAMPLE 1

In wastewater treatment, the first step is the preparation of a biostimulated composition that represents Phase 1 - Biostimulation.

The preparation of the biostimulated compound comprises: folic acid (100 grams), sodium bicarbonate (100 ml 0.5% solution, ammonium chloride (100 grams), granulated calcium chloride (100 ml 0.05% solution) ), magnesium chloride hexahydrate (50 grams), potassium chloride (50 grams), sodium chloride (200 grams), meat extract (250 grams), yeast extract (100 grams), casein peptone extract (100 grams) grams), bacteriological peptone (100 grams), monobasic potassium phosphate (50 grams), vitamin complex (25 mL) and chlorine-free filtered water (50 liters).

In the previously described compound are added the microorganisms that can be selected from the phylum protozoa, from the classes: sarcodyne, mastigophora, ciliata, rotifera, nematodes and phyllo annular.

The microorganisms are selected according to the application of interest which comprises field application in pollution control, wastewater treatment (in wastewater treatment plants), soil treatment and other applications (CONAMA 314).

For the treatment of effluent of domestic origin the most suitable is to use the biostimulated compound that preferably comprises the microorganisms selected from the filamentous bacteria, Beggiatoa Alba, Bacillus sp, Bacillus licheniformis, Bacillus amyloliquefaceas, Rhodospirillum rubrum, Aquaspirillum gracille. , rotifers: Brachionus, Keratella and protozoa: Paramecium, Lionotus,, Pseudomonas stutzeri,, Escherichia hermanii, Escherichia lincheniformis Paenibacillus polymyxa, Pseudomonas fluorescens, and Pseudomonas putida,, Aquaspirillum gracille, Rotifera: Lioness

After preparation of the biostimulation compound, it is stored for a period between 36 and 72h, preferably 48h, prior to application in the effluent.

EXAMPLE 2 Phase 2 (Bioaccumulation) consists of using the biostimulated compound produced in Phase 1 (Biostimulation - contains essential nutrients, salts, vitamins and the presence of microorganisms). The bio-augmentation process utilizes the activated compound in volumes of 6 liters and in these are added components comprising: 2000 to 3000 grams, preferably 2500 grams of brown sugar or similar low industrial process, 1500 to 2500 grams, preferably 2000 grams of tripolyphosphate of food grade sodium, 500 to 1500 grams, preferably 1000 grams of urea, 500 to 1500 liters, and preferably 1000 liters of chlorine free water.

The compost is then homogenized and then applied to the effluent treatment, with a large scale and low cost "biological sanitation" function.

The compound should preferably be at an alkaline pH (varying in range from acid to alkaline, but close to its neutrality) and its application is by the use of a doser at the points indicated in the treatment systems, and may be in the systems primary, secondary and tertiary in wastewater treatment systems according to their specificity and process type. Whether in accumulation tanks, grease boxes, equalizers, biological reactors and biological filters, whether aerobic or anaerobic.

The volume is gradually added 24 hours a day, directly in the biological reactors, of the effluent treatment system. Its volumes are related to the type of pollutant and volume of effluent to be treated, as well as the technology adopted for the treatment system, in which we have the application of this process in wastewater treatment systems, covering almost all sources of liquid effluents.

Claims

1. "BIO-STIMULATION AND BIO-INCREASING PROCESSES FOR TREATMENT OF DOMESTIC AND INDUSTRIAL WASTE", characterized by the process of effluent treatment in water treatment plants include the following steps:

(a) preparation of a biostimulated composition; b) Addition of microorganisms in the biostimulated composition of step (a) for activation; and

c) Application of activated biostimulated compound in the effluent to initiate the bioaccumulation process.

Biostimulated composition according to Claim 1, characterized in that it comprises: 50 to 150 g, preferably 100 g of folic acid; 50 to 150 mL, preferably 100 mL sodium bicarbonate (0.5% concentration); 50 to 150g, preferably 100g of ammonium chloride; 50 to 150mL, preferably 100mL of granulated calcium chloride (concentration 0.05%); 25 to 75g, preferably 50g of magnesium chloride hexahydrate; 25 to 75g, preferably 50g of potassium chloride; 150 to 250g, preferably 200g of sodium chloride; 200 to 300g, preferably 250g of meat extract; 50 to 150g, preferably 100g of yeast extract; 50 to 150g, preferably 100g casein peptone extract; 50 to 150g, preferably 100g of bacteriological peptone; 25 to 75g, preferably 50g of monobasic potassium phosphate; 12.5mL to 37.5mL, preferably 25mL of vitamin complex, and 50L of chlorine-free filtered water.

Composition according to Claim 1, characterized in that the microorganisms are selected from: Beggiatoa Alba, Bacillus sp, Bacillus licheniformis, Bacillus subtillis, Bacillus amyloliquefaceas, Rhodospirillum rubrum, Aquaspirillum gracille, Brachionus, Keratella, Paramecium, Lionotus, Pseudomonas stutzeri.

Process according to Claim 1, characterized in that the biostimulated composition of step (a) is stored for a period of between 36h and 72h, preferably 48h, prior to application to the effluent to be treated.

Process according to Claim 1, characterized in that the activation (bioaccumulation) step of the biostimulated compound comprises: 2000 to 3000 grams, preferably 2500 grams, of brown sugar; 1500 to 2500 grams, preferably 2000 grams of food grade sodium tripolyphosphate; 500 to 1500 grams, preferably 1000 grams of urea; 500 to 1500 liters, preferably 1000 liters of chlorine free water.

Process according to Claim 1, characterized in that the pH of the biostimulated composition is between 7.5 and 9, preferably 8.

Process according to Claim 1, characterized in that the source of the effluent is domestic or industrial.

Process according to Claim 7, characterized in that the industrial effluent comes from processes for the production of: paper and pulp, the production of paints, inks, dairy products, oil and other segments.

Process according to claim 8, characterized by the industrial effluent coming from production processes of the most diverse productive segments.