CN109105237A - Remove and measure the experimental method that wetland plant rhizosphere microorganism group influences phosphorus in plant absorption water - Google Patents
Remove and measure the experimental method that wetland plant rhizosphere microorganism group influences phosphorus in plant absorption water Download PDFInfo
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- CN109105237A CN109105237A CN201810692391.6A CN201810692391A CN109105237A CN 109105237 A CN109105237 A CN 109105237A CN 201810692391 A CN201810692391 A CN 201810692391A CN 109105237 A CN109105237 A CN 109105237A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
- A01G31/02—Special apparatus therefor
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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Abstract
The invention discloses a kind of removal and wetland plant rhizosphere microorganism group is measured on the experimental method of phosphorus influence in plant absorption water, it is related to field of biotechnology, the removal and measure wetland plant rhizosphere microorganism group in plant absorption water phosphorus influence experimental method include 1. removal wetland plant rhizosphere microorganisms, 2. plant culture solution is prepared, 3. the plant for removing rhizosphere microorganism cultivates three steps of comparative experiments, by comparing phosphorus concentration in several groups of culture solutions, obtaining the difference between the two is influence of the wetland plant rhizosphere microorganism group to phosphorus in plant absorption water, objective can reliably study various rhizosphere microorganism groups influences phosphorus in plant absorption water, excellent technological guidance is provided to improve plant to phosphorus absorption.
Description
Technical field
The present invention relates to water environment restoration of the ecosystem field, in particular to a kind of removal simultaneously measures wetland plant rhizosphere microorganism
The experimental method that group influences phosphorus in plant absorption water.
Background technique
Phosphorus is one of element necessary to plant growth, and leads to the limitative nutrient of water eutrophication.It is wet
There are complicated substance conversion and cyclic process in the ground ecosystem, the effects of to the retention of pollutant, absorption, absorption, degradation
Pollutant can be efficiently controlled and enter the water bodys such as river, lake.Many efforts at environmental protection are dedicated to using wetland high-efficiency going dephosphorization
Pollution, especially in terms of controlling agricultural non-point source pollution.Plant in wetland plays an important role in terms of absorbing and going dephosphorization.
However, the phosphorus of most of form cannot directly be absorbed by plant in environment, only become available phosphorus by decomposition and inversion, it could quilt
Plant absorption, wetland plant rhizosphere microorganism play an important role in the process.However existing research and no-trump wetland rhizosphere
Its influence that phosphorus is absorbed to the conversion of phosphorus in water and to wetland plant as a whole, is studied by microbiologic population.Lead to not
Various microorganisms to phosphorus in plant absorption water and promote wetland to phosphorus in objective reliably research wetland rhizosphere microorganism group
The influence of the removal of pollution.
The present invention is that research and utilization wetland rhizosphere microorganism group raising plant dephosphorization efficiency proposes new experimental method, to mention
High wetland plant phosphor-removing effect prevents and treats water eutrophication for wetland, restoration of the ecosystem provides new method and new approaches.
Summary of the invention
Technical problem to be solved by the invention is to provide a kind of removal and measure wetland plant rhizosphere microorganism group pair
The experimental method that phosphorus influences in plant absorption water objective can reliably study various wetland rhizosphere microorganisms group and inhale to plant
Receiving phosphorus in water influences, and provides excellent technological guidance to improve wetland plant to phosphorus absorption.
To achieve the above object, the present invention provides technical solution below:
The removal and measure wetland plant rhizosphere microorganism group in plant absorption water phosphorus influence experimental method include
Following steps:
(1) wetland plant rhizosphere microorganism is removed；
(2) plant culture solution is prepared；
(3) plant for removing rhizosphere microorganism cultivates comparative experiments.
Preferably, the removal wetland plant rhizosphere microorganism includes the following steps:
(1) natural bioremediation plant is acquired, is first tentatively rinsed well whole plant with tap water before experiment, soil is not stayed in root
Earth；
(2) it is connect until there is no brown soil trace on cotton balls with the cotton balls wiping plant roots and stem of distillation water-soaked
Plant root is immersed in 75% alcohol 10~15 minutes (with Ethanol Exposure total time be no more than 20 minutes, so as not to wound
Evil plant influences follow-up cultivation experiment), in aseptic operating platform, the plant impregnated is taken out from alcohol, rapidly with sterile
Water rinses out residual alcohol, in gnotobasis, picks 75% alcohol with cotton balls and continues the root of careful wiping plant comprehensively
Must be 3 times, after nuzzling up with aseptic water washing or residual alcohol is wiped out to get the plant being removed to rhizosphere microorganism at once；
(3) whether it is removed clean for verifying rhizosphere microorganism, takes 250mL conical flask, distilled water 100mL is added, in 121
It sterilizes 20 minutes in high-pressure sterilizing pot at DEG C, is cooled to room temperature, the conical flask equipped with sterile water after being sterilized, then will carry out
The plant of removal rhizosphere microorganism after aforesaid operations, takes one plant of plant aseptically, with the scissors or pocket knife to sterilize
Its root 2g is cut, is put into the sterilizing conical flask equipped with 100mL sterile water, is sealed, is placed on sterilizing tampon or sealed membrane
Taken out after being shaken 30 minutes on isothermal vibration device, take wherein 1mL shake extracting solution be aseptically uniformly coated on and made
On the beef extract-peptone solid medium to sterilize got ready, it is then placed in constant incubator at 30 DEG C and cultivates 48 hours
After observe, if without bacterium colony grow, illustrate that rhizosphere microorganism has removed completely.
Preferably, the plant culture solution preparation includes the following steps:
(1) plant culture solution main component: phosphorus drug purity, which reaches, to be analyzed more than pure, with tricalcium phosphate (Ca3(PO4)2) or
Potassium dihydrogen phosphate (KH2PO4) or lecithin, the concentration in terms of phosphorus, phosphorus content is configured in the culture solution of 0~5mg/L；Plant culture
Liquid raw water, acquisition water quality reaches or Group III standard is natively better than " water environment quality standard " GB3838-2002)
Table water；Urea and glucose；
(2) first phosphorus-containing compound medicament, such as potassium dihydrogen phosphate are let cool in drier after 110 DEG C of dry 2h, is used in combination
Assay balance weighs 0.0877g (corresponding phosphorus quality 20mg), then weighs 0.214g urea, and 2.0g glucose adds distilled water to dissolve,
Constant volume is made into the phosphorus stock solution of 20mg/L into 1000mL volumetric flask again；
(3) " phosphorus stock solution (20mg/L) " is cultivated with the phosphorus plant that " plant culture solution raw water " is diluted to 0~5mg/L
Liquid.
Preferably, the plant culture comparative experiments of the removal rhizosphere microorganism includes the following steps:
(1) natural bioremediation plant is acquired, is first tentatively rinsed well whole plant with tap water before experiment, soil is not stayed in root
Earth chooses wherein well-grown, and calamus number plant similar in growing way slightly cleans, and trims dead twigs and withered leaves, surveys the measurement of experiment front and back
Plant diameter and height: measuring the diameter of calamus, measurement height with vernier caliper, and each plant is measured 3 times and is averaged, tests
Front and back measures total phosphorus content in plant；
(2) a part of plant is cooked into removal rhizosphere microorganism processing, another part does not remove rhizosphere microorganism, it is real to do comparison
It tests, takes 1000mL conical flask, be added above-mentioned " phosphorus plant culture solution ", the calamus handled well is inserted, to eliminating rhizosphere
The plant of microorganism blocks taper bottleneck (group 1) with the absorbent cotton package plant to sterilize, does not remove the plant of rhizosphere microorganism
It is put into conical flask, operation is same as above, but does not have to block up (group 2), is cultivated 10~15 days at clean, ventilation, light transmission, is measured culture solution
Middle phosphorus concentration, each phosphorus concentration do 3 in parallel, and phosphorus concentration in 2 culture solutions of comparative group 1 and group, obtaining the difference between the two is wetland
Influence of the plant rhizosphere microbe group to phosphorus in plant absorption water.
Be using the beneficial effect of above technical scheme: the removal simultaneously measures wetland plant rhizosphere microorganism group to plant
Absorbing the experimental method that phosphorus influences in water includes 1. removal wetland plant rhizosphere microorganisms, the preparation of 2. plant culture solutions, 3. removals
The plant of rhizosphere microorganism cultivates three steps of comparative experiments, by comparing total phosphorus concentration in several groups of culture solutions, obtains the two
Difference is influence of the wetland plant rhizosphere microorganism group to phosphorus in plant absorption water, objective can reliably study various wetlands
Rhizosphere microorganism group influences phosphorus in plant absorption water, provides excellent technological guidance to improve wetland plant to phosphorus absorption.
Plant dephosphorization efficiency is improved for research and utilization wetland rhizosphere microorganism group, new experimental method is provided, removed with improving wetland plant
Phosphorus effect prevents and treats water eutrophication for wetland, restoration of the ecosystem provides new method and new approaches.
Detailed description of the invention
A specific embodiment of the invention is described in further detail with reference to the accompanying drawing.
Fig. 1 is the plant culture schematic diagram for removing rhizosphere microorganism.
Specific embodiment
The invention will now be described in detail with reference to the accompanying drawings it is a kind of removal and measure wetland plant rhizosphere microorganism group to plant
Absorb the preferred embodiment for the experimental method that phosphorus influences in water.
Fig. 1, which shows a kind of removal of the present invention and measures wetland plant rhizosphere microorganism group, influences phosphorus in plant absorption water
Experimental method specific embodiment:
The removal and measure wetland plant rhizosphere microorganism group in plant absorption water phosphorus influence experimental method include
Following steps:
1, the removal of wetland plant rhizosphere microorganism
1.1 experimental plant.It acquires natural bioremediation plant (such as calamus), first with tap water tentatively by whole plant before experiment
It rinses well, soil is not stayed in root.
1.2 removal rhizosphere microorganism groups
Then plant roots and stem are wiped with the cotton balls of distillation water-soaked, until there is no brown soil trace on cotton balls.
Then plant root is immersed in 75% alcohol 10~15 minutes (with Ethanol Exposure total time be no more than 20 minutes, in order to avoid
Plant is injured, follow-up cultivation experiment is influenced).In aseptic operating platform, the plant impregnated is taken out from alcohol, uses nothing rapidly
Bacterium water rinses out residual alcohol.In gnotobasis, 75% alcohol is picked with cotton balls and continuation carefully wipes plant comprehensively
Root 3 times, with aseptic water washing or residual alcohol is wiped out at once after nuzzling up.Obtain the plant that rhizosphere microorganism is removed.
Whether 1.3 verifying rhizosphere microorganisms are removed clean
Whether it is removed clean for verifying rhizosphere microorganism, takes 250mL conical flask, distilled water 100mL is added, in 121 DEG C
It sterilizes 20 minutes in lower high-pressure sterilizing pot, is cooled to room temperature, the conical flask equipped with sterile water after being sterilized.On carrying out again
The plant of removal rhizosphere microorganism after stating operation, takes one plant of plant aseptically, is cut with the scissors or pocket knife that sterilized
Lower its root 2g, is put into the sterilizing conical flask equipped with 100mL sterile water.With sterilizing tampon or sealed membrane sealing, it is placed on perseverance
It is taken out after being shaken 30 minutes on warm oscillator.Take wherein 1mL shake extracting solution be aseptically uniformly coated on and prepared
On the good beef extract-peptone solid medium to sterilize, it is then placed in constant incubator after being cultivated 48 hours at 30 DEG C
Observation illustrates that rhizosphere microorganism has removed completely if grown without bacterium colony.
2, plant culture solution is prepared
The influence of phosphorus is absorbed to study rhizosphere microorganism to wetland plant.Prepare wetland plant culture solution.
2.1 plant culture solution main components
1) phosphorus.Drug purity, which reaches, to be analyzed more than pure.With tricalcium phosphate (Ca3(PO4)2) or potassium dihydrogen phosphate (KH2PO4) or
Lecithin, the concentration in terms of phosphorus.Phosphorus content is configured in the culture solution of 0~5mg/L.
2) plant culture solution raw water.Acquisition water quality reaches or better than " water environment quality standard " (GB3838-
2002) natural surface water (such as lake water, river water, river) of Group III standard.
3) urea and glucose.
2.2, plant culture solution preparation steps:
It first prepares a), then is diluted a) with above-mentioned raw water 2) with certain proportion, is made into b).
A) phosphorus stock solution (20mg/L) is prepared.First by phosphorus-containing compound medicament, if potassium dihydrogen phosphate is after 110 DEG C of dry 2h
It is let cool in drier, and weighs 0.0877g (corresponding phosphorus quality 20mg) with assay balance, then weigh 0.214g urea, 2.0g
Glucose adds distilled water to dissolve, then constant volume is made into the phosphorus stock solution of 20mg/L into 1000mL volumetric flask.
B) phosphorus plant culture solution." a) phosphorus stock solution (20mg/L) " is diluted to 0 with " 2) plant culture solution raw water "~
The phosphorus plant culture solution of 5mg/L.
Such as:
900mL " 2) plant culture solution raw water " plus 100mL " a) phosphorus stock solution (20mg/L) " is taken, shakes up, is made into 2mg/L
" b) phosphorus plant culture solution "；
950mL " 2) plant culture solution raw water " plus 50mL " a) phosphorus stock solution (20mg/L) " is taken, shakes up, is made into 1mg/L
" b) phosphorus plant culture solution ".
3, the plant for removing rhizosphere microorganism cultivates comparative experiments
3.1 choose well-grown in 1.1, and calamus number plant similar in growing way slightly cleans, trims dead twigs and withered leaves.It surveys real
It tests front and back measurement plant diameter and height: measuring the diameter of calamus, measurement height with vernier caliper, each plant is measured 3 times and taken
Average value.Total phosphorus content in experiment front and back measurement plant.
Plant a part of in 3.1 is cooked removal rhizosphere microorganism processing (group 1) by 3.2, and another part does not remove the micro- life of rhizosphere
Object (group 2), does comparative experiments.
1000mL conical flask is taken, is added above-mentioned " b) phosphorus plant culture solution ", the calamus handled well is inserted.To removal
The plant of rhizosphere microorganism blocks taper bottleneck with the absorbent cotton package plant to sterilize, as shown in Figure 1, (group 1).It does not go
Except the plant of rhizosphere microorganism is put into conical flask, operation is same as above, but does not have to block up (group 2).It is cultivated at clean, ventilation, light transmission
10~15 days, measure phosphorus concentration in culture solution.Each phosphorus concentration does 3 in parallel.Phosphorus concentration in 2 culture solutions of comparative group 1 and group, obtains
It is influence of the wetland plant rhizosphere microorganism group to phosphorus in plant absorption water to the difference between the two.
The above are merely the preferred embodiment of the present invention, it is noted that for those of ordinary skill in the art,
Without departing from the concept of the premise of the invention, various modifications and improvements can be made, these belong to guarantor of the invention
Protect range.
Claims (4)
1. a kind of removal simultaneously measures the experimental method that wetland plant rhizosphere microorganism group influences phosphorus in plant absorption water, special
Sign is: it is described removal and measure wetland plant rhizosphere microorganism group in plant absorption water phosphorus influence experimental method include
Following steps:
(1) wetland plant rhizosphere microorganism is removed；
(2) plant culture solution is prepared；
(3) plant for removing rhizosphere microorganism cultivates comparative experiments.
2. removal according to claim 1 simultaneously measures wetland plant rhizosphere microorganism group and influences on phosphorus in plant absorption water
Experimental method, it is characterised in that: the removal wetland plant rhizosphere microorganism includes the following steps:
(1) natural bioremediation plant is acquired, is first tentatively rinsed well whole plant with tap water before experiment, soil is not stayed in root；
(2) then will until there is no brown soil trace on cotton balls with the cotton balls wiping plant roots and stem of distillation water-soaked
Plant root, which is immersed in 75% alcohol 10~15 minutes, (to be no more than 20 minutes with Ethanol Exposure total time, plants in order to avoid injuring
Object influences follow-up cultivation experiment), in aseptic operating platform, the plant impregnated is taken out from alcohol, is rushed with sterile water rapidly
Residual alcohol is washed off, in gnotobasis, 75% alcohol is picked with cotton balls and continues the root 3 of careful wiping plant comprehensively
Time, after nuzzling up with aseptic water washing or residual alcohol is wiped out to get the plant being removed to rhizosphere microorganism at once；
(3) whether it is removed clean for verifying rhizosphere microorganism, takes 250mL conical flask, distilled water 100mL is added, at 121 DEG C
It sterilizes 20 minutes in high-pressure sterilizing pot, is cooled to room temperature, the conical flask equipped with sterile water after being sterilized, then will carry out above-mentioned
The plant of removal rhizosphere microorganism after operation, takes one plant of plant aseptically, is cut with the scissors or pocket knife that sterilized
Its root 2g is put into the sterilizing conical flask equipped with 100mL sterile water, is sealed with sterilizing tampon or sealed membrane, is placed on constant temperature
Taken out after being shaken 30 minutes on oscillator, take wherein 1mL shake extracting solution be aseptically uniformly coated on and prepared
The beef extract-peptone solid medium to sterilize on, be then placed in constant incubator after being cultivated 48 hours at 30 DEG C and see
It examines, if grown without bacterium colony, illustrates that rhizosphere microorganism has removed completely.
3. removal according to claim 1 simultaneously measures wetland plant rhizosphere microorganism group and influences on phosphorus in plant absorption water
Experimental method, it is characterised in that: plant culture solution preparation includes the following steps:
(1) plant culture solution main component: phosphorus drug purity, which reaches, to be analyzed more than pure, with tricalcium phosphate (Ca3(PO4)2) or phosphoric acid
Potassium dihydrogen (KH2PO4) or lecithin, the concentration in terms of phosphorus, phosphorus content is configured in the culture solution of 0~5mg/L；Plant culture solution is former
Expect water, acquisition water quality reaches or better than " water environment quality standard " GB3838-2002) natural surface water of Group III standard；
Urea and glucose；
(2) first phosphorus-containing compound medicament, such as potassium dihydrogen phosphate are let cool in drier after 110 DEG C of dry 2h, and with analysis
Balance weighs 0.0877g (corresponding phosphorus quality 20mg), then weighs 0.214g urea, and 2.0g glucose adds distilled water to dissolve, then fixed
Hold in 1000mL volumetric flask, is made into the phosphorus stock solution of 20mg/L；
(3) " phosphorus stock solution (20mg/L) " is diluted to the phosphorus plant culture solution of 0~5mg/L with " plant culture solution raw water ".
4. removal according to claim 1 simultaneously measures wetland plant rhizosphere microorganism group and influences on phosphorus in plant absorption water
Experimental method, it is characterised in that: it is described removal rhizosphere microorganism plant culture comparative experiments include the following steps:
(1) natural bioremediation plant is acquired, is first tentatively rinsed well whole plant with tap water before experiment, soil is not stayed in root,
Wherein well-grown is chosen, calamus number plant similar in growing way slightly cleans, and trims dead twigs and withered leaves, surveys the measurement of experiment front and back and plants
Object diameter and height: the diameter of calamus is measured with vernier caliper, measurement height, each plant is measured 3 times and is averaged, before experiment
Total phosphorus content in plant is measured afterwards；
(2) a part of plant being cooked into removal rhizosphere microorganism processing, another part does not remove rhizosphere microorganism, does comparative experiments,
It takes 1000mL conical flask, is added above-mentioned " phosphorus plant culture solution ", the calamus handled well is inserted, to eliminating the micro- life of rhizosphere
The plant of object blocks taper bottleneck (group 1) with the absorbent cotton package plant to sterilize, and the plant for not removing rhizosphere microorganism is put into
Conical flask, operation are same as above, but do not have to block up (group 2), are cultivated 10~15 days at clean, ventilation, light transmission, are measured phosphorus in culture solution
Concentration, each phosphorus concentration do 3 in parallel, and phosphorus concentration in 2 culture solutions of comparative group 1 and group, obtaining the difference between the two is wetland plant
Influence of the rhizosphere microorganism group to phosphorus in plant absorption water.
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| CN201810692391.6A CN109105237A (en) | 2018-06-26 | 2018-06-26 | Remove and measure the experimental method that wetland plant rhizosphere microorganism group influences phosphorus in plant absorption water |
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|---|---|---|---|
| CN201810692391.6A CN109105237A (en) | 2018-06-26 | 2018-06-26 | Remove and measure the experimental method that wetland plant rhizosphere microorganism group influences phosphorus in plant absorption water |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102010065A (en) * | 2010-10-08 | 2011-04-13 | 天津水工业工程设备有限公司 | Comprehensive treatment method of landscape water eutrophication and algae overgrowth |
| CN102154142A (en) * | 2010-10-20 | 2011-08-17 | 湖南大学 | Microorganism for promoting black nightshade to remove trace cadmium pollution in water and method for removing cadmium pollution |
| CN102757132A (en) * | 2012-07-31 | 2012-10-31 | 山东大学 | Method for treating rural domestic sewage by utilizing denitrifying phosphate-accumulating organisms and invigorated artificial wetland |
| CN103172177A (en) * | 2013-03-27 | 2013-06-26 | 杭州绿风园林建设集团有限公司 | Method for improving repairing capacity of eutrophic body of water of plants by microorganisms |
| CN103923868A (en) * | 2014-04-30 | 2014-07-16 | 山东大学 | Mixed flora microbial preparation and application thereof in sewage treatment |
| CN104974954A (en) * | 2015-06-19 | 2015-10-14 | 贵州省草业研究所 | Application of grass phosphorus-dissolving plant growth promoting rhizobacterium |
| CN106517531A (en) * | 2016-12-30 | 2017-03-22 | 徐州工程学院 | Method for improving water purifying effect of aquatic plant |
-
2018
- 2018-06-26 CN CN201810692391.6A patent/CN109105237A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102010065A (en) * | 2010-10-08 | 2011-04-13 | 天津水工业工程设备有限公司 | Comprehensive treatment method of landscape water eutrophication and algae overgrowth |
| CN102154142A (en) * | 2010-10-20 | 2011-08-17 | 湖南大学 | Microorganism for promoting black nightshade to remove trace cadmium pollution in water and method for removing cadmium pollution |
| CN102757132A (en) * | 2012-07-31 | 2012-10-31 | 山东大学 | Method for treating rural domestic sewage by utilizing denitrifying phosphate-accumulating organisms and invigorated artificial wetland |
| CN103172177A (en) * | 2013-03-27 | 2013-06-26 | 杭州绿风园林建设集团有限公司 | Method for improving repairing capacity of eutrophic body of water of plants by microorganisms |
| CN103923868A (en) * | 2014-04-30 | 2014-07-16 | 山东大学 | Mixed flora microbial preparation and application thereof in sewage treatment |
| CN104974954A (en) * | 2015-06-19 | 2015-10-14 | 贵州省草业研究所 | Application of grass phosphorus-dissolving plant growth promoting rhizobacterium |
| CN106517531A (en) * | 2016-12-30 | 2017-03-22 | 徐州工程学院 | Method for improving water purifying effect of aquatic plant |
Non-Patent Citations (11)
| Title |
|---|
| BARBER等: "Influence of micro-organisms on the distribution in roots of phosphate labelled with phosphorus-32", 《NATURE》 * |
| CAO等: "Phosphorus Solubilizing and Releasing Bacteria Screening from the Rhizosphere in a Natural Wetland", 《WATER》 * |
| 周世玲等: "菖蒲对污水中氮及磷的净化效应 ", 《北方园艺》 * |
| 周晓坤等: "水菖蒲内生菌分离与抗菌活性菌株筛选 ", 《食品科学》 * |
| 周等: "4种不同生活型湿地植物对富营养化水体的净化效果 ", 《应用生态学报》 * |
| 夏宏生等: "人工湿地除磷技术 ", 《四川环境》 * |
| 庞海东等: "耐重金属的植物促生芽孢杆菌筛选及其强化香蒲去除Cd的作用", 《农业环境科学学报》 * |
| 朱培淼等: "高效解磷细菌的筛选及其对玉米苗期生长的促进作用 ", 《应用生态学报》 * |
| 李旭东等: "《废水处理技术及工程应用》", 30 June 2003, 机械工业出版社 * |
| 毕银丽等: "接种菌根对根际微生物群落和磷营养的影响 ", 《能源环境保护》 * |
| 韦巧等: "不同磷水平下印度梨形孢对生菜生长及磷素吸收利用的影响 ", 《河南农业科学》 * |
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