CN101343114A - Biological denitrification method for wastewater of industrial cultivation - Google Patents
Biological denitrification method for wastewater of industrial cultivation Download PDFInfo
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- CN101343114A CN101343114A CNA2008101392071A CN200810139207A CN101343114A CN 101343114 A CN101343114 A CN 101343114A CN A2008101392071 A CNA2008101392071 A CN A2008101392071A CN 200810139207 A CN200810139207 A CN 200810139207A CN 101343114 A CN101343114 A CN 101343114A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
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Abstract
The invention discloses a biological denitrogenation method of factory culture wastewater. The method is to build a submerged type upflow membrane bioreactor to transform ammonia nitrogen in factory culture wastewater through enriching acclimation heterotrophic nitrifiers and utilizing a porous carrier to adsorb and fix the separated heterotrophic nitrifiers, thereby efficiently and continuously decomposing and transforming ammonia nitrogen, nitrite nitrogen and organics having rather big poisoning effects to the factory culture wastewater, and reducing the total nitrogen content of culture water and the pollution to the surrounding environment of cultivation. The method for continuously restoring the factory culture wastewater and built in situ has the advantages of low investment, simple operation and high efficiency, and is suitable for the wastewater treatment of nitrifying units in factory extensive cultivation.
Description
Technical field:
The invention belongs to technical field of waste water processing, especially relate to the method for the biological denitrificaion of batch production high-density breeding waste water.
Background technology:
Culture fishery is avoided the overdeveloped important means of marine fishing resource as alleviating human demand pressure to food.The culture fishery of China enters the stage of growing continuously and fast, the intensification of breeding way height appears aquaculture self-pollution problem gradually and is outstanding day by day, cause breeding environment to worsen, disease is popular, has caused enormous economic loss for whole culture fishery and blindly use microbiotic for control disease.The resource that recirculated water high-density industrialized culture takies is few, and can produce commercial fishery products the whole year, environmentally friendly, is the direction of culture fishery development.The high-density industrialized culture mainly has higher requirement to cultivation water environment, must remove organism such as residual bait, ight soil fast, and the ammonia nitrogen that will produce fast, nitrite nitrogen remain on lower level simultaneously.
The method of breeding wastewater denitrogenation at present is a lot, wherein bio-denitrification technology have investment and running expense lower, and advantage such as non-secondary pollution is most economical effective means.Biological denitrificaion is mainly finished by three kinds of modes, and the one, utilize the nitrogenous compound in the photosynthesis assimilation breeding wastewater of green plants, realize culturing the circulation and the processing of water, its maximum shortcoming is that efficient is low, floor space is big.The 2nd, utilize the nitrifier of autotrophy and anaerobic denitrifying bacteria that ammonia nitrogen is changed into nitrite nitrogen, nitric nitrogen, be converted into nitrogen at last and finish the whole process of biological denitrificaion, this method is the main method that present high-density wastewater of industrial cultivation is handled, its disadvantage is that the autotrophic bacteria of finishing nitrification belongs to chemosynthetic bacteria, specific growth rate is lower, the aquaculture water acidifying, in not having the unitary treatment system of denitrification, often cause the accumulation of nitric nitrogen, the biology and the environment of culturing produced adverse influence, denitrification requires the anaerobic environment simultaneously, aquaculture water is the environment of oxygen enrichment, and difficulty is bigger in actual motion.The third method of biological denitrificaion is a heterotrophic nitrification, directly utilizes the assimilation of bacterium to transform, absorbs the nitrogenous substances in the water body, and technology is simple, the efficient height of conversion, but the separation screening difficulty of allotrophic nitrobacteria, and the biomass that nitrifying process produces is big.CN03118598.3 has narrated the method for separating allotrophic nitrobacteria in soil, also set forth sieve method of heterotrophic nitrifying bacteria in the Chinese patent application 200510030456.3 and 200610010263.6, the disadvantage of three kinds of methods is directly separation screening from physical environment, microbe species is many, and workload is bigger.The largest benefit of utilizing allotrophic nitrobacteria to carry out the breeding wastewater denitrogenation is the accumulation that does not have nitrite nitrogen and nitric nitrogen in treatment system, removes organism simultaneously, is the biological denitrificaion mode that application prospect is arranged.
The factor that influences the growth of autotrophy or allotrophic nitrobacteria and play a role mainly comprises quantity and the existing way of pH, basicity, temperature, DO, ammonia nitrogen, salinity, nitrobacteria.Microbe population under the suspension growth state is few, easily runs off, and therefore adopts immobilization technologies in the process of carrying out biological denitrificaion more, and optimizes the optimum process condition of allotrophic nitrobacteria biological denitrificaion.
The high-density industrialized culture is the trend of culture fishery development, and in the North America, West Europe, Japan, the high-density industrial circulating water has become comparatively sophisticated industry, is mainly used in to produce famous and precious fishery products.The high-density industrialized culture of China has certain development, but is subjected to the restriction of economic level and water quality control techniques, and the water treatment of the suitable China's actual conditions of exploitation and reuse technology are to promoting the key factor of batch production high-density breeding in China.
Summary of the invention:
Purpose of the present invention transforms the inorganic nitrogen compound in the wastewater of industrial cultivation, thereby realizes the circulation of breeding wastewater, and reduces the harm to environment.The present invention adopts method enrichment, the domestication ocean nitrobacteria of progressively improving ammonia nitrogen concentration, and the absorption that utilizes porous support to finish the ocean allotrophic nitrobacteria is fixed, set up upper reaches submerged membrane bioreactor sea farming waste water is carried out biological denitrificaion, has less investment, the characteristics that coastal breed factory is easy to carry out, not only reduce the nitrogenous inorganics in the aquaculture water, and COD is also had certain removal effect.Method of the present invention realizes by following steps:
1, biological denitrification method for wastewater of industrial cultivation is characterized in that it carries out as follows:
(1), enrichment domestication ocean allotrophic nitrobacteria: the enrichment domestication of ocean allotrophic nitrobacteria is carried out in the container of certain volume, in container, add certain volume sea farming waste water, the water body volume should account for more than 3/4 of container volume, and the dissolved oxygen of control water body is used Na more than 3 mg/L
2CO
3Solution is regulated pH between 7.5-8.5, regulate salinity about 1.020, the control water temperature is at 25 ± 2 ℃, the COD of regulation and control water body is in the scope of 5-50 mg/L, the ammonium chloride solution that every day, stream added 0.2-2g/L carries out the enrichment domestication, the loss of total nitrogen appears in the domestication through 20-30 days in the inorganic nitrogen mensuration process, just can isolate allotrophic nitrobacteria from the enrichment domestication liquid;
(2), the separation of ocean allotrophic nitrobacteria and enlarged culturing: the ocean allotrophic nitrobacteria separates the SOLID ORGANIC substratum that adopts the seawater preparation, the enrichment domestication liquid is coated on the SOLID ORGANIC substratum through serial dilution, cultivated 3 days for 23-27 ℃, the different bacterium colony of the colony characteristics of growing on the substratum is rule behind the purifying, carry out the nitrated aptitude tests of single strain, until being separated to the ocean allotrophic nitrobacteria, adopt the method for solid and liquid to enlarge the strong allotrophic nitrobacteria of nitrated ability, the number 10 of bacterium
8Individual/more than the mL;
(3), the absorption of ocean allotrophic nitrobacteria is fixed: the porous support that the ocean allotrophic nitrobacteria of enlarged culturing is added bacterium liquid and sterilization in sizeable triangular flask, temperature is controlled at 25 ± 2 ℃, rotating speed is between 80-150 rpm, absorption is 48h fixedly, the carrier that shaking table absorption is fixed is packed in the bio-reactor, utilize same bacterium liquid to continue to reflux and adsorb 24h, HRT is in the scope of 1-2h, adopt the upflowing water intake mode, water body floods whole filtrate volume, sets up upper reaches submerged membrane bioreactor;
(4), carry out the wastewater of industrial cultivation biological denitrificaion: after the ocean allotrophic nitrobacteria has been fixed in carrier absorption in the submerged membrane bioreactor of upper reaches, change ammonia nitrogen concentration (0.2-0.8mg/L), hydraulic detention time (15-150 minute), COD size (5-20mg/L), working time various factorss such as (12-48 hours), each change all determines the nitric efficiency of upper reaches submerged membrane bioreactor, when denitrification effect in 1 hour reaches 50% when above, setting up a factory breeding wastewater biological denitrification method is described.
Embodiment:
Below in conjunction with embodiment the present invention is described further:
Embodiment 1:
(1), allotrophic nitrobacteria enrichment domestication: the enrichment domestication of allotrophic nitrobacteria is carried out in aquarium, and the aquarium volume is 60 L.Breeding wastewater 40 L that in cylinder, add long-term aeration.。The pH of solution 1M Na
2CO
3Solution is regulated, and is controlled at (environment pH value can constantly reduce, and illustrates that nitrobacteria is carrying out nitration reaction fast in the cylinder) between the 7.5-8.5, regulates salinity about 1.020 ± 0.01 with fresh water, raises to reduce the salinity that causes owing to moisture evaporation.Built-in temperature regulating heater control water temperature is at 25 ± 2 ℃; Miniature water circulating pump is by dissolved oxygen (the Dissolved Oxygen in the mobile increase water body of water, DO) more than 5 mg/L, the COD of regulation and control water body is between 20 ± 5 mg/L, the ammonium chloride solution that every day, stream added 0.5 g/L carries out the enrichment domestication, in case stablizing, rate of nitrification (ammonia nitrogen rate of oxidation and nitrous attitude rate of oxidation) just improves the ammonia nitrogen amount that stream adds, with the ammonia nitrogen concentration of solution from 0.5 mg/L, bring up to 1,2,4,6 mg/L successively, domestication through 45d has reached 8 mg/L at last.When the nitrite nitrogen oxidation capacity of system was stablized, the content of nitric nitrogen all can obviously reduce.The content of total inorganic nitrogen is along with the carrying out of domestication process in the system, and the gap of itself and total addition level is increasing, i.e. occur the loss of inorganic nitrogen in the domestication process, just can isolate allotrophic nitrobacteria from the enrichment domestication liquid.
(2) separation of ocean allotrophic nitrobacteria and enlarged culturing: at first prepare the substratum that ocean allotrophic nitrobacteria separation screening is used: (NH
4)
2SO
40.1g; MgSO
47H
2O 0.03g; NaH
2PO
40.25g; K
2HPO
40.75g; Na
2CO
30.5g; MnSO
44H
2O 0.01g; Glucose 0.1 gram, peptone 0.1 gram, Chen Haishui 100 0mL; PH 7.5-8.5, agar powder 16 grams, 121 ℃ of sterilization 20 min.The enrichment domestication liquid is coated on the SOLID ORGANIC substratum through serial dilution, cultivate 3d for 25 ± 2 ℃, the different bacterium colony of the colony characteristics of growing on the substratum is rule behind the purifying, carry out the nitrated aptitude tests of single strain, utilize the plate streaking method to obtain the bacterial strain to be selected of solid culture, getting 2 encircles in the liquid nutrient medium that is inoculated in the claim 3,25 ± 2 ℃, shaking table 160 rmp rotating speeds are cultivated 24-48 h down, measure the reduction of ammonia nitrogen, compared with the control, this bacterial strain of judgement that ammonia nitrogen reduces more than 20% has the heterotrophic nitrification ability, until being separated to the strong bacterial strain of ocean heterotrophic nitrification ability.The fluid enlargement culture of preparation ocean allotrophic nitrobacteria: (NH
4)
2SO
40.05g; MgSO
47H
2O 0.03g; NaH
2PO
40.25g; K
2HPO
40.75g; Na
2CO
30.5g; MnSO
44H
2O 0.01 g; Glucose 0.05 gram, peptone 0.05 gram, Chen Haishui 100 0mL; PH 7.5-8.5,121 ℃ of sterilization 20 mi n, 25 ± 2 ℃, shaking table 140-200 rmp rotating speed is cultivated 36 h down, and number of bacteria reaches 2*10
8Individual/mL.
(3), the absorption of ocean allotrophic nitrobacteria fixes: utilize the fluid enlargement culture base of ocean allotrophic nitrobacteria to carry out the enlarged culturing of bacterium, the number 2*10 of bacterium
8Individual/mL, in the 500mL triangular flask, add the sterilization big ball of PVA (d=4 mm) respectively, PVA bead (d=2 mm), the PVA sizes of balls of volume equivalent, each adds the ocean allotrophic nitrobacteria bacterium liquid liquid of 200 milliliters enlarged culturing, temperature is controlled at 25 ± 2 ℃, rotating speed is between 120 rpm, absorption is 48h fixedly, the carrier that shaking table absorption the is fixed bio-reactor of packing into is (high 45 centimetres, 4.5 centimetres of internal diameters) in, utilize same bacterium liquid to continue to reflux and adsorb 24h, HRT adopts the upflowing water intake mode in the scope of 1-2h, water body floods whole filtrate volume, sets up upper reaches submerged membrane bioreactor;
(4), the establishment of biological denitrification method for wastewater of industrial cultivation processing condition: after the ocean allotrophic nitrobacteria has been fixed in carrier absorption in the submerged membrane bioreactor of upper reaches, determine that this upper reaches submerged bio-reactor carries out the optimal condition of biological denitrificaion, the optimum ammonia nitrogen concentration of this reactor is 0.8 mg/L, the performance that PVA mixes goalpost obviously is better than big goalpost of PVA and the little goalpost of PVA, it is 5mg/L that PVA mixes the strongest COD value of the nitrated ability of goalpost, the nitrated ability of hydraulic detention time PVA mixing goalpost when 1h is best, and the nitrated ability of mixing goalpost reaches 26.86 gram ammonia nitrogen/m
3/ d, under the various conditions, the ammonia nitrogen removal frank of the large and small ball of PVA, mixing ball is respectively at 65-79% and 60-86% and 69-92%, and this explanation is setting up a factory breeding wastewater biological denitrification method.
Embodiment 2:
(1), allotrophic nitrobacteria enrichment domestication: the enrichment domestication of allotrophic nitrobacteria is carried out in aquarium, and the aquarium volume is 40 L.Breeding wastewater 30 L that in cylinder, add long-term aeration.。The pH of solution 1M Na
2CO
3Solution is regulated, and is controlled at (environment pH value can constantly reduce, and illustrates that nitrobacteria is carrying out nitration reaction fast in the cylinder) between the 7.5-8.5, regulates salinity about 1.020 ± 0.01 with fresh water, raises to reduce the salinity that causes owing to moisture evaporation.Built-in temperature regulating heater control water temperature is at 25 ± 2 ℃; Miniature water circulating pump is by dissolved oxygen (the Dissolved Oxygen in the mobile increase water body of water, DO) more than 5 mg/L, the COD of regulation and control water body is between 20 ± 5 mg/L, the ammonium chloride solution that per 12 hours stream adds 0.5 g/L carries out the enrichment domestication, in case stablizing, rate of nitrification (ammonia nitrogen rate of oxidation and nitrous attitude rate of oxidation) just improves the ammonia nitrogen amount that stream adds, with the ammonia nitrogen concentration of solution from 0.5 mg/L, bring up to 1,2,3,4,6,8,10 mg/L successively, domestication through 30d has reached 10 mg/L at last.When the nitrite nitrogen oxidation capacity of system was stablized, the content of nitric nitrogen all can obviously reduce.The content of total inorganic nitrogen is along with the carrying out of domestication process in the system, and the gap of itself and total addition level is increasing, i.e. occur the loss of inorganic nitrogen in the domestication process, just can isolate allotrophic nitrobacteria from the enrichment domestication liquid.
(2) separation of ocean allotrophic nitrobacteria and enlarged culturing: at first prepare the substratum that ocean allotrophic nitrobacteria separation screening is used: (NH
4)
2SO
40.1 g; MgSO
47H
2O 0.03 g; NaH
2PO
40.25g; K
2HPO
40.75g; Na
2CO
30.5g; MnSO
44H
2O 0.01g; Glucose 0.1 gram, peptone 0.1 gram, Chen Haishui 100 0mL; PH 7.5-8.5, agar powder 16 grams, 121 ℃ of sterilization 20 min.The enrichment domestication liquid is coated on the SOLID ORGANIC substratum through serial dilution, cultivate 3d for 25 ± 2 ℃, the different bacterium colony of the colony characteristics of growing on the substratum is rule behind the purifying, carry out the nitrated aptitude tests of single strain, utilize the plate streaking method to obtain the bacterial strain to be selected of solid culture, getting 2 encircles in the liquid nutrient medium that is inoculated in the claim 3,25 ± 2 ℃, shaking table 130 rmp rotating speeds are cultivated 24-48 h down, measure the reduction of ammonia nitrogen, compared with the control, this bacterial strain of judgement that ammonia nitrogen reduces more than 20% has the heterotrophic nitrification ability, until being separated to the strong bacterial strain of ocean heterotrophic nitrification ability.The fluid enlargement culture of preparation ocean allotrophic nitrobacteria: (NH
4)
2SO
40.05g; MgSO
47H
2O 0.03g; NaH
2PO
40.25g; K
2HPO
40.75g; Na
2CO
30.5g; MnSO
44H
2O 0.01 g; Glucose 0.05 gram, peptone 0.05 gram, Chen Haishui 100 0mL; PH 7.5-8.5,121 ℃ of sterilization 20 min, 25 ± 2 ℃, shaking table 140-200 rmp rotating speed is cultivated 36 h down, and number of bacteria reaches 2*10
8Individual/mL.
(3), the absorption of ocean allotrophic nitrobacteria fixes: utilize the fluid enlargement culture base of ocean allotrophic nitrobacteria to carry out the enlarged culturing of bacterium, the number 2*10 of bacterium
8Individual/mL, in the 500mL triangular flask, add columnar activated carbon (h=2 mm, 1 mm), 200 milliliters of the ocean nitrobacterias of adding enlarged culturing, temperature is controlled at 26 ± 2 ℃, rotating speed is between 100 rpm, absorption is 48h fixedly, the carrier that shaking table absorption is fixed is packed in the bio-reactor (high 45 centimetres, 4.5 centimetres of internal diameters), utilizes the same bacterium liquid absorption 24h that continues to reflux, HRT is in the scope of 1-2h, adopt the upflowing water intake mode, water body floods whole filtrate volume, sets up upper reaches submerged membrane bioreactor;
(4), the establishment of biological denitrification method for wastewater of industrial cultivation processing condition: after the ocean allotrophic nitrobacteria has been fixed in carrier absorption in the submerged membrane bioreactor of upper reaches, determine that this upper reaches submerged bio-reactor carries out the optimal condition of biological denitrificaion, the optimum ammonia nitrogen concentration of this reactor is 0.8 mg/L, the COD value that the nitrated ability of activated carbon column is the strongest is 15 mg/L, hydraulic detention time nitrated ability of activated carbon column when 1h is best, and the nitrated ability of activated carbon column reaches 27.48 gram ammonia nitrogen/m
3/ d, ammonia-N removal rate is at 63-89% under the various conditions, and this explanation is setting up a factory breeding wastewater biological denitrification method.
Claims (6)
1, biological denitrification method for wastewater of industrial cultivation is characterized in that it carries out as follows:
(1), enrichment domestication ocean allotrophic nitrobacteria: the enrichment domestication of ocean allotrophic nitrobacteria is carried out in the container of certain volume, in container, add certain volume sea farming waste water, the water body volume should account for more than 3/4 of container volume, and the dissolved oxygen of control water body is used Na more than 3mg/L
2CO
3Solution is regulated pH between 7.5-8.5, regulate salinity about 1.020, the control water temperature is at 25 ± 2 ℃, the COD of regulation and control water body is in the scope of 5-50mg/L, the ammonium chloride solution that every day, stream added 0.2-2g/L carries out the enrichment domestication, the loss of total nitrogen appears in the domestication through 20-30 days in the inorganic nitrogen mensuration process, just can isolate allotrophic nitrobacteria from the enrichment domestication liquid;
(2), the separation of ocean allotrophic nitrobacteria and enlarged culturing: the ocean allotrophic nitrobacteria separates the SOLID ORGANIC substratum that adopts the seawater preparation, the enrichment domestication liquid is coated on the SOLID ORGANIC substratum through serial dilution, cultivated 3 days for 23-27 ℃, the different bacterium colony of the colony characteristics of growing on the substratum is rule behind the purifying, carry out the nitrated aptitude tests of single strain, until being separated to the ocean allotrophic nitrobacteria, adopt the method for solid and liquid to enlarge the strong allotrophic nitrobacteria of nitrated ability, the number 10 of bacterium
8Individual/more than the mL;
(3), the absorption of ocean allotrophic nitrobacteria is fixed: the porous support that the ocean allotrophic nitrobacteria of enlarged culturing is added bacterium liquid and sterilization in sizeable triangular flask, temperature is controlled at 25 ± 2 ℃, rotating speed is between 80-150rpm, absorption is 48h fixedly, the carrier that shaking table absorption is fixed is packed in the bio-reactor, utilize same bacterium liquid to continue to reflux and adsorb 24h, HRT is in the scope of 1-2h, adopt the upflowing water intake mode, water body floods whole filtrate volume, sets up upper reaches submerged membrane bioreactor;
(4), carry out the wastewater of industrial cultivation biological denitrificaion: after the ocean allotrophic nitrobacteria has been fixed in carrier absorption in the submerged membrane bioreactor of upper reaches, change ammonia nitrogen concentration (0.2-0.8mg/L), hydraulic detention time (15-150 minute), COD size (5-20mg/L), working time various factorss such as (12-48 hours), each change all determines the nitric efficiency of upper reaches submerged membrane bioreactor, when denitrification effect in 1 hour reaches 50% when above, setting up a factory breeding wastewater biological denitrification method is described.
2, according to the described biological denitrification method for wastewater of industrial cultivation of claim 1, the enrichment acclimation method that it is characterized in that said ocean allotrophic nitrobacteria is: in the container of certain volume, add the breeding wastewater more than 3/4, the recirculated water aeration guarantees dissolved oxygen more than 3mg/L, with the Na of 1M
2CO
3Solution is regulated pH between 7.5-8.5, regulate salinity about 1.020 with fresh water, the control water temperature is at 25 ± 2 ℃, and the COD of regulation and control water body is in the scope of 5-50mg/L, and the ammonium chloride solution that every day, stream added 0.5g/L carries out the enrichment domestication, ammonia nitrogen concentration is adjusted to lower concentration, but be not less than 0.5mg/L, when nitrite nitrogen concentration begins to descend, progressively improve the addition of ammonia nitrogen, when the enrichment domestication finished, the ammonia nitrogen that add every day was no more than 10mg/L.Through the above domestication of 20d, the loss of total nitrogen appears in the inorganic nitrogen mensuration process, just can from the enrichment domestication liquid, isolate allotrophic nitrobacteria.
3, according to the described biological denitrification method for wastewater of industrial cultivation of claim 1, it is characterized in that adopting selective medium separating marine allotrophic nitrobacteria, the preparation method of allotrophic nitrobacteria selective medium is as follows: (NH
4)
2SO
40.1g; MgSO
47H
2O 0.03g; NaH
2PO
40.25g; K
2HPO
40.75g; Na
2CO
30.5g; MnSO
44H
2O 0.01g; Glucose 0.1 gram, peptone 0.1 gram, Chen Haishui 1000mL; PH 7.5-8.5, agar powder 16 grams, 121 ℃ of sterilization 20min.
4, according to claim 1 or 3 described biological denitrification method for wastewater of industrial cultivation, it is characterized in that adopting liquid fermenting to measure the method for its heterotrophic nitrification ability, utilize the plate streaking method to obtain the bacterial strain to be selected of solid culture, getting 2 rings is inoculated in the described liquid nutrient medium, 25 ± 2 ℃, shaking table 140-200rmp rotating speed is cultivated 24-48h down, measures the reduction of ammonia nitrogen, compared with the control, ammonia nitrogen minimizing this bacterial strain of judgement more than 20% has the heterotrophic nitrification ability.
5, according to the described biological denitrification method for wastewater of industrial cultivation of claim 1, it is characterized in that the fluid enlargement culture of ocean allotrophic nitrobacteria: the fluid enlargement culture of preparation ocean allotrophic nitrobacteria: (NH
4)
2SO
40.05g; MgSO
47H
2O 0.03g; NaH
2PO
40.25g; K
2HPO
40.75g; Na
2CO
30.5g; MnSO
44H
2O 0.01g; Glucose 0.05 gram, peptone 0.05 gram, Chen Haishui 1000mL; PH 7.5-8.5,121 ℃ of sterilization 20min, 25 ± 2 ℃, shaking table 140-200rmp rotating speed is cultivated 24-48h down.
6, according to the described biological denitrification method for wastewater of industrial cultivation of claim 1, it is characterized in that the selection of carrier, the specific surface area of carrier will be at 600m
2More than/the g, proportion guarantees natural subsidence in seawater between 1.022-1.030, and it is mobile to suspend under water impact simultaneously, so that slough the aged microbial film.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102259975A (en) * | 2011-05-27 | 2011-11-30 | 甘肃金桥给水排水设计与工程(集团)有限公司 | Bio-augmentation technique for treating high ammonia nitrogen waste water under low temperature condition |
| CN103896410A (en) * | 2014-04-21 | 2014-07-02 | 中国海洋大学 | Biofilm-based mariculture wastewater treatment system |
| CN109090007A (en) * | 2018-08-02 | 2018-12-28 | 魏雪珠 | A kind of light, the full ecology filtration system of seawater race fishpond shrimp ponds production method |
| CN113651432A (en) * | 2021-09-01 | 2021-11-16 | 江苏五湖生态环境科技有限公司 | Microbial biofilm carrier for removing ammonia nitrogen in water body and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4720344A (en) * | 1985-04-18 | 1988-01-19 | Ganczarczyk Jerzy J | Nitrification process in waste water treatment |
| CN1597566A (en) * | 2004-07-26 | 2005-03-23 | 北京交通大学 | Sewage treatment method using sequential batch type reactor |
| CN101200332A (en) * | 2006-12-12 | 2008-06-18 | 上海水产大学 | Method for removing nitrate nitrogen from aquaculture water |
-
2008
- 2008-08-16 CN CN200810139207A patent/CN101343114B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4720344A (en) * | 1985-04-18 | 1988-01-19 | Ganczarczyk Jerzy J | Nitrification process in waste water treatment |
| CN1597566A (en) * | 2004-07-26 | 2005-03-23 | 北京交通大学 | Sewage treatment method using sequential batch type reactor |
| CN101200332A (en) * | 2006-12-12 | 2008-06-18 | 上海水产大学 | Method for removing nitrate nitrogen from aquaculture water |
Non-Patent Citations (2)
| Title |
|---|
| 王光玉等: "海洋硝化菌群的富集驯化和筛选的初步研究", 《海洋水产研究》 * |
| 苏俊峰等: "异氧硝化细菌处理氨氮废水及微生物群落结构分析", 《北京工业大学学报》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102259975A (en) * | 2011-05-27 | 2011-11-30 | 甘肃金桥给水排水设计与工程(集团)有限公司 | Bio-augmentation technique for treating high ammonia nitrogen waste water under low temperature condition |
| CN103896410A (en) * | 2014-04-21 | 2014-07-02 | 中国海洋大学 | Biofilm-based mariculture wastewater treatment system |
| CN103896410B (en) * | 2014-04-21 | 2016-05-25 | 中国海洋大学 | A kind of based on biomembranous culturing wastewater processing system |
| CN109090007A (en) * | 2018-08-02 | 2018-12-28 | 魏雪珠 | A kind of light, the full ecology filtration system of seawater race fishpond shrimp ponds production method |
| CN113651432A (en) * | 2021-09-01 | 2021-11-16 | 江苏五湖生态环境科技有限公司 | Microbial biofilm carrier for removing ammonia nitrogen in water body and preparation method thereof |
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| CN101343114B (en) | 2012-09-05 |
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