CN113292164B - Preparation method and application of phycomycete symbiont for degrading culture wastewater containing high-concentration antibiotics and having low C/N ratio - Google Patents
Preparation method and application of phycomycete symbiont for degrading culture wastewater containing high-concentration antibiotics and having low C/N ratio Download PDFInfo
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Abstract
A preparation method and application of an algae-bacteria symbiont for degrading culture wastewater containing high-concentration antibiotics and having a low C/N ratio. The invention belongs to the field of water treatment. The invention aims to solve the technical problems of long treatment time, high cost and poor treatment effect caused by the complex process of biologically treating the aquaculture wastewater by using the conventional activated sludge and the incapability of synchronously performing each treatment process. The method comprises the following steps: firstly, culturing chlorella to logarithmic phase, and centrifuging to obtain chlorella cell sediment; then performing specific domestication on the activated sludge, and centrifuging to obtain activated sludge sediment; and mixing the two solutions uniformly to obtain the phycomycete symbiont for degrading the culture wastewater containing high-concentration antibiotics and having low C/N ratio. The application comprises the following steps: the obtained phycomycete symbiont is used for treating culture waste water containing high concentration antibiotic and low C/N ratio. The algae-bacteria symbiotic system can achieve a good nitrogen and phosphorus removal effect in a short period, is simple and easy to operate, has low preparation cost and has the potential of large-scale popularization and application.
Description
Technical Field
The invention belongs to the field of water treatment, and particularly relates to a preparation method and application of an algae-bacteria symbiont for degrading culture wastewater containing high-concentration antibiotics and having a low C/N ratio.
Background
China is a big livestock and poultry breeding country and generates a large amount of livestock and poultry wastewater every year. The water contains a large amount of organic matters (COD), nitrogen, phosphorus, veterinary drug residues and the like, has complex components and high pollution load, is accompanied by foul smell, and is high-concentration organic wastewater. After the livestock and poultry breeding water is discharged into the surrounding water body, a large amount of oxygen in the water is consumed, so that aquatic organisms die and the water body smells; the high concentration of nitrogen and phosphorus contained in the wastewater can also cause eutrophication of the water body. Although a plurality of physical and chemical methods are available for treating sewage, the method is not enough in treating breeding wastewater, and a method for efficiently treating the breeding wastewater is lacked.
The microalgae is an autotroph with wide distribution and strong adaptability, and the microalgae and the metabolites thereof can be used in the aspects of biopharmaceuticals, natural food processing, sustainable energy production and the like, and have wide market prospects. However, there is a problem that the cultivation cost is high. The culture wastewater is treated by using the microalgae, so that the culture cost of the microalgae can be reduced, the aim of recycling the sewage can be fulfilled, and the wide attention of scientific researchers can be easily aroused. However, the technology for treating aquaculture wastewater by microalgae is still imperfect at present, and still faces the problems that the aquaculture wastewater has high ammonia nitrogen content, and the growth of microalgae is inhibited.
At present, the domestic water treatment mode is mainly based on biological treatment of activated sludge. However, with the substantial increase of nitrogen and phosphorus in the aquaculture water, the eutrophication of the water body is more and more serious, the technical process of treating the aquaculture wastewater based on the activated sludge is more complicated, specifically comprises the processes of nitrification, denitrification, aerobic phosphorus absorption, anaerobic phosphorus release and the like, and the reaction conditions are different, so that the treatment process cannot be carried out synchronously, the floor area and the treatment time of a sewage treatment plant are greatly increased, the cost is increased, and a good nitrogen and phosphorus removal effect cannot be achieved.
Disclosure of Invention
The invention aims to solve the technical problems of long treatment time, high cost and poor treatment effect caused by the fact that the existing activated sludge biological treatment process for aquaculture wastewater is complex and each treatment process cannot be synchronously carried out, and provides a preparation method and application of an algae bacterium symbiont for degrading aquaculture wastewater containing high-concentration antibiotics and having a low C/N ratio.
The preparation method of the phycomycete symbiont for degrading the culture wastewater containing high-concentration antibiotics and low C/N ratio comprises the following steps:
step 1: adding Chlorella pyrenoidosa into BG-11 culture medium, then pre-culturing in a photobioreactor, and culturing to logarithmic phase to obtain Chlorella liquid; wherein the parameters for the pre-culture in the photobioreactor are: the culture temperature is 29-32 ℃, the culture rotating speed is 200-300 rpm, and the culture illumination is 150 mu mol/m2/s～300μmol/m2The aeration rate is 1L/min-2L/min;
step 2: centrifuging the chlorella solution obtained in the step 1, removing supernatant, washing the centrifuged chlorella solution with distilled water to obtain chlorella cell sediment, centrifuging again, removing supernatant to obtain chlorella cell sediment;
and step 3: continuously aerating activated sludge by using an air pump, and then domesticating by using artificial simulated culture wastewater to obtain an activated sludge suspension of a specific microbial community;
and 4, step 4: centrifuging the activated sludge suspension of the specific microbial community obtained in the step 3 to remove supernatant, washing the centrifuged activated sludge precipitate with distilled water, centrifuging again to remove supernatant to obtain activated sludge precipitate;
and 5: and (4) uniformly mixing the chlorella cell sediment obtained in the step (2) and the activated sludge sediment obtained in the step (4) to obtain the phycomycete symbiont for degrading the culture wastewater containing high-concentration antibiotics and having low C/N ratio.
Further defined, the gas aerated in step 1 is CO2Mixed gas with air, CO in the mixed gas2Is 2 percent.
Further limiting, the dry weight of the chlorella in the chlorella liquid obtained in the step 1 in the step 2 is calculated by an OD linear relation equation, wherein the OD linear relation equation is as follows: the dry weight (g/L) of Chlorella in Chlorella solution is 0.2519 XOD680+0.0123，R2＝0.9972。
Further limiting, the artificial simulated aquaculture wastewater in the step 3 is aquaculture wastewater containing antibiotics and having a low C/N ratio, and the specific components are pH: 7.25. + -. 0.05, COD: 620 mg/L-650 mg/L, NO3 --N：40mg/L～45mg/L、NH4 +-N：420mg/L～450mg/L、TP：12mg/L～16mg/L。
Further defined, the microbial community in the activated sludge of the specific microbial community obtained in step 3 includes Proteobacteria, bacteroida, Cyanobacteria, bdellovibrotota, firmutes, Actinobacteriota, myxococca, acidobacteria, Verrucomicrobiota.
Further defined, the gas for continuous aeration in the step 3 is CO2Mixed gas with air, CO in the mixed gas2Is 2 percent.
Further, the mass ratio of the chlorella cell sediment obtained in the step 2 in the step 5 to the activated sludge sediment obtained in the step 4 is (1-10): 1.
The phycomycete symbiont for degrading culture wastewater containing high-concentration antibiotics and low C/N ratio is used for treating the culture wastewater containing high-concentration antibiotics and low C/N ratio.
Further defined, the method for treating the cultivation wastewater containing the high concentration antibiotic and the low C/N ratio with the algae bacterium symbiont degrading the cultivation wastewater containing the high concentration antibiotic and the low C/N ratio is as follows:
inoculating the phycomycete symbiont degrading the culture waste water containing high concentration antibiotic and low C/N ratio into the culture waste water to be treated, culturing at 29-32 deg.c and at 200-300 rpm under 150 μmol/m illumination2/s～300μmol/m2And/s and the aeration amount is 1-2L/min.
Further limiting, from the beginning of inoculation, adjusting the pH value of the water body to be neutral every 12 h.
Compared with the prior art, the invention has the advantages that:
1) chlorella pyrenoidosa belongs to Chlorophyta, is widely distributed in nature and is easy to culture;
2) the addition of the microalgae strengthens the removal capability of the activated sludge on nitrogen and phosphorus;
3) the algae-bacteria symbiotic system can achieve good nitrogen and phosphorus removal effect in a short period;
4) the operation is simple and easy, and the preparation cost is low;
5) the algae-bacteria symbiotic system can play a role in degrading high-concentration antibiotics in the culture wastewater, so that the algae-bacteria symbiotic system has great advantages and has the potential of large-scale popularization and application.
Drawings
FIG. 1 is a graph of the linear relationship of OD in step 2 of one embodiment;
FIG. 2 shows the ratio of algal bacteria symbiont to NH in the second embodiment4 +-degradation profile of N;
FIG. 3 is a graph of biodiversity at different antibiotic concentrations in example 2;
FIG. 4 shows NH in aquaculture wastewater by the pure activated sludge system of the algal bacteria symbiont of example 1 and control 3 in example 34 +-N removal effect graph;
FIG. 5 is a graph showing the effect of the pure activated sludge system of the algal bacteria symbiont of example 1 and the control group 3 in removing TP in the aquaculture wastewater in example 3;
FIG. 6 shows the effect of the pure activated sludge system of the algal bacteria symbiont of example 4 and the control group 4 on NH in aquaculture wastewater in example 54 +-N removal effect graph;
FIG. 7 is a graph showing the effect of the pure activated sludge system of the algal bacteria symbiont of example 4 and the control group 4 in removing TP in the aquaculture wastewater in example 5;
FIG. 8 is a graph showing the effect of the pure activated sludge system of the phycomycete symbiont of example 4 and the control group 4 in removing sulfamethoxazole from aquaculture wastewater in example 5;
FIG. 9 shows the effect of the pure activated sludge system of the algal bacteria symbiont of example 4 and the control group 5 on NH in aquaculture wastewater in example 64 +-N removal effect graph;
FIG. 10 is a graph showing the effect of the pure activated sludge system of the algal bacteria symbiont of example 4 and the control group 5 in example 6 on the removal of TP in the aquaculture wastewater.
Detailed Description
The first embodiment is as follows: the preparation method of the phycomycete symbiont for degrading the culture wastewater containing high-concentration antibiotics and low C/N ratio of the embodiment comprises the following steps:
step 1: chlorella pyrenoidosa is added to BG-11 medium and then pre-cultured in a photobioreactor to log phase, i.e., OD on day four680Obtaining chlorella liquid within the range of 6-7; wherein the parameters for the pre-culture in the photobioreactor are: the culture temperature is 30 ℃, the culture rotating speed is 200rpm, and the culture illumination is 200 mu mol/m2The aeration rate is 1.5L/min; the aerated gas is CO2And air, the mixed gas is CO2The mass fraction of (A) is 2%; wherein the BG-11 medium has the composition shown in Table 1;
TABLE 1 BG-11 Medium composition
Step 2: centrifuging the chlorella solution obtained in the step 1 at 8000rpm for 5min, discarding the supernatant, washing the centrifuged chlorella cell precipitate with distilled water, centrifuging at 8000rpm for 5min, discarding the supernatant to obtain chlorella cell precipitate; the dry weight of the chlorella in the chlorella liquid obtained in the step 1 is calculated by an OD linear relation equation (shown in figure 1), wherein the OD linear relation equation is as follows: dry weight of Chlorella in Chlorella solution(g/L)＝0.2519×OD680+0.0123，R2＝0.9972；
And step 3: continuously aerating activated sludge by using an air pump, and then domesticating by using artificial simulated culture wastewater to obtain an activated sludge suspension of a specific microbial community; the aerated gas is CO2And air, the mixed gas is CO2The mass fraction of (A) is 2%; the artificial simulated aquaculture wastewater is aquaculture wastewater containing antibiotics and having a low C/N ratio, and the specific components of the artificial simulated aquaculture wastewater are shown in Table 2;
TABLE 2 Artificial simulated wastewater composition
The microbial community in the activated sludge obtained at a concentration of 171mg/30mL of a particular microbial community includes, but is not limited to: phylum Proteobacteria (Proteobacteria), bacteroidetes (bacteroidata), Cyanobacteria (cyanoobacteria), bdellovibrionotta, Firmicutes (Firmicutes), actinomycetes (actinobacteria), myxococca, acidobacterium (acidobacteria), Verrucomicrobiota (Verrucomicrobiota);
and 4, step 4: centrifuging the activated sludge suspension of the specific microbial community obtained in the step 3 at 8000rpm for 5min, discarding the supernatant, washing the centrifuged activated sludge precipitate with distilled water, centrifuging at 8000rpm for 5min, discarding the supernatant, and obtaining the activated sludge precipitate;
and 5: and (3) respectively and uniformly mixing the chlorella cell sediment obtained in the step (2) and the activated sludge sediment obtained in the step (4) according to the mass ratio of the dry weight of the chlorella cell sediment to the dry weight of 1:1, 5:1 and 10:1 to obtain the phycomycete symbiont for degrading the culture wastewater containing high-concentration antibiotics and having low C/N ratio.
The second embodiment is as follows: the phycomycete symbiont for degrading culture wastewater containing high-concentration antibiotics and low C/N ratio is used for treating the culture wastewater containing high-concentration antibiotics and low C/N ratio, and the specific treatment method comprises the following steps:
inoculating the algal bacteria symbiont of the first embodiment to the aquaculture wastewater to be treatedWherein the culture temperature is 29 to 32 ℃, the culture rotation speed is 200 to 300rpm, and the culture illumination is 150 mu mol/m2/s～300μmol/m2The treatment is carried out under the condition that the aeration quantity is 1L/min-2L/min, and the pH value of the water body is adjusted to be neutral every 12 hours from the beginning of inoculation; wherein the composition of the aquaculture wastewater to be treated is shown in Table 2.
Detection of NH by UV absorption4 +N, obtaining the algal bacteria symbiont pair NH under different proportions as shown in figure 24 +N degradation profile, as can be seen from fig. 2, by day 2 of culture, compared to 10:1 and 1:1, the algae-bacteria ratio is 5: symbiotic system of 1 to NH4 +the-N degradation is already evident.
Example 1: the preparation method of the phycomycete symbiont for degrading the culture wastewater containing high-concentration antibiotics and low C/N ratio in the embodiment comprises the following steps:
step 1: chlorella pyrenoidosa is added to BG-11 medium and then pre-cultured in a photobioreactor to log phase, i.e., OD on day four680The range is 6.627, and chlorella liquid is obtained; wherein the parameters for the pre-culture in the photobioreactor are: the culture temperature is 30 ℃, the culture rotating speed is 200rpm, and the culture illumination is 200 mu mol/m2The aeration rate is 1.5L/min; the aerated gas is CO2And air, the mixed gas is CO2The mass fraction of (A) is 2%; wherein the BG-11 medium has the composition shown in Table 1;
step 2: centrifuging the chlorella solution obtained in the step 1 at 8000rpm for 5min, discarding the supernatant, washing the centrifuged chlorella cell precipitate with distilled water, centrifuging at 8000rpm for 5min, discarding the supernatant to obtain chlorella cell precipitate; the dry weight of the chlorella in the chlorella liquid obtained in the step 1 is calculated by an OD linear relation equation (shown in figure 1), wherein the OD linear relation equation is as follows: the dry weight (g/L) of Chlorella in Chlorella solution is 0.2519 XOD680+0.0123，R20.9972; the dry weight of the chlorella in the chlorella solution is calculated to be 1.6816 g/L;
and 3, step 3: sewage treatment plant (Harbin city Taiping sewage)Treating plant) continuously aerating activated sludge by using an air pump, then domesticating by using artificial simulated culture wastewater, drying at 105 ℃, and weighing to obtain an activated sludge suspension of a specific microbial community with the concentration of 171mg/30 mL; the aerated gas is CO2And air, the mixed gas is CO2The mass fraction of (A) is 2%; the artificial simulated aquaculture wastewater is aquaculture wastewater containing antibiotics and having a low C/N ratio, and the specific components of the artificial simulated aquaculture wastewater are shown in Table 2;
the microbial communities in the activated sludge obtained for a particular microbial community include, but are not limited to: phylum Proteobacteria (Proteobacteria), bacteroidetes (bacteroidata), Cyanobacteria (cyanoobacteria), bdellovibrionotta, Firmicutes (Firmicutes), actinomycetes (actinobacteria), myxococca, acidobacterium (acidobacteria), Verrucomicrobiota (Verrucomicrobiota);
and 4, step 4: centrifuging the activated sludge suspension of the specific microbial community obtained in the step 3 at 8000rpm for 5min, discarding the supernatant, washing the centrifuged activated sludge precipitate with distilled water, centrifuging at 8000rpm for 5min, discarding the supernatant, and obtaining the activated sludge precipitate;
and 5: and (3) uniformly mixing the chlorella cell sediment obtained in the step (2) and the activated sludge sediment obtained in the step (4) according to the mass ratio of the dry weight of the chlorella cell sediment to the dry weight of the activated sludge sediment to be 5:1 to obtain the phycomycete symbiont for degrading the culture wastewater containing high-concentration antibiotics and having low C/N ratio.
Example 2: the cultivation wastewater shown in Table 2 was treated with the algal bacteria symbiont obtained in example 1, which degrades cultivation wastewater containing high concentration antibiotics and having a low C/N ratio, and 10mg/L sulfamethoxazole was added to the cultivation wastewater, specifically, the treatment method was as follows:
the algal fungus symbiont of example 1 was inoculated at a dry weight of 500mg/L into 10mg/L sulfamethoxazole culture wastewater at a culture temperature of 30 ℃ and a culture rotation speed of 200rpm under a culture illumination of 200. mu. mol/m2The aeration rate is 1.5L/min, and the aerated gas is CO2And air, the mixed gas is CO2The mass fraction of the water is 2 percent, and the pH value of the water body is adjusted to be neutral every 12 hours from the beginning of inoculation.
The activated sludge culture system of the sewage treatment plant (Taiping sewage treatment plant in Harbin city) in the step 3 is used as a control group 1, and the culture wastewater without the sulfamethoxazole is used as a control group 2, and the activated sludge culture system and the culture wastewater are cultured under the same culture conditions as the example 2.
The biodiversity detection adopts a MiSeq-based high-throughput sequencing technology to obtain a biodiversity curve chart shown in figure 3 under different antibiotic concentrations, and as can be seen from figure 3, when the sulfamethoxazole concentration is 10mg/L, the biodiversity increase in the phycobiont system of example 1 is very obvious compared with that in a control group 2 without sulfamethoxazole added after 5 days of culture; the increase in biodiversity in the phycobiont system of example 1 was also evident compared to activated sludge control group 1.
Example 3: the cultivation wastewater shown in Table 2 was treated with the algal bacteria symbiont degrading cultivation wastewater containing high concentration antibiotics and having a low C/N ratio obtained in example 1, and the specific treatment method was as follows:
the algal fungus symbiont of example 1 was inoculated at a dry weight of 500mg/L into the culture wastewater shown in Table 2, and cultured at a culture temperature of 30 ℃ and a culture rotation speed of 200rpm under a culture illumination of 200. mu. mol/m2The treatment is carried out under the conditions that the aeration rate is 1.5L/min and the aerated gas is CO2And air, the mixed gas is CO2The mass fraction of the water is 2 percent, and the pH value of the water body is adjusted to be neutral every 12 hours from the beginning of inoculation.
The activated sludge culture system of the sewage treatment plant (Taiping sewage treatment plant in Harbin city) in the step 3 is used as a control group 3, and the activated sludge culture system is cultured under the same culture conditions as those in the example 3.
NH in the water body treated in example 1 and the control group 3 by the Nassler reagent method4 +Detection of-N to obtain NH in the aquaculture wastewater by the pure activated sludge system of the algal bacteria symbiont of example 1 and the control group 3 as shown in FIG. 44 +FIG. 4 shows NH pair of the symbiotic system of phycomycetes in example 1 after 5 days of cultivation compared with the activated sludge of control group 34 +The removal effect of-N is very obvious.
The molybdenum-antimony spectrophotometry method is adopted to detect TP in the water bodies treated in the embodiment 1 and the control group 3, a curve diagram of the removing effect of the phycomycete symbiont in the embodiment 1 and the pure activated sludge system in the control group 3 on TP in the aquaculture wastewater shown in fig. 5 is obtained, and as can be seen from fig. 5, after the culture for 1d, compared with the activated sludge in the control group 3, the removing effect of the phycomycete symbiont system in the embodiment 1 on TP is very obvious. At the time of 3d, the TP content in the phycobiont system of example 1 was reduced to 0.
Example 4: this example differs from example 1 in that: OD of culture to logarithmic phase, i.e., fourth day, in step 1680The range is 6.0732, and the dry weight of chlorella in chlorella liquid is calculated to be 1.5421g/L in step 2. The other steps and parameters were the same as in example 1.
Example 5: the cultivation wastewater shown in Table 2 was treated with the algal bacteria symbiont obtained in example 4, which degrades cultivation wastewater containing high concentration antibiotics and having a low C/N ratio, and 10mg/L sulfamethoxazole was added to the cultivation wastewater, specifically, the treatment method was as follows:
the algal fungus symbiont of example 4 was inoculated at a dry weight of 500mg/L into sulfamethoxazole culture wastewater at a concentration of 10mg/L, and the culture temperature was 30 ℃ and the culture rotation speed was 200rpm, and the culture illumination was 200. mu. mol/m2The aeration rate is 1.5L/min, and the aerated gas is CO2Mixed gas of CO and air2The mass fraction of the water is 2 percent, and the pH value of the water body is adjusted to be neutral every 12 hours from the beginning of inoculation.
The activated sludge culture system of the sewage treatment plant (Taiping sewage treatment plant in Harbin city) in the step 3 is used as a control group 4, and the activated sludge culture system is cultured under the same culture conditions as those in the example 5.
NH in the water body treated by the example 4 and the control group 4 by adopting an ultraviolet absorption method4 +Detection of-N to obtain NH in the aquaculture wastewater by the pure activated sludge system of the algal bacteria symbiont of example 4 and the control group 4 as shown in FIG. 64 +FIG. 6 shows the effect of-N removal, and after 5d of cultivation, the activated sludge was compared with that of control 4Algal symbiotic System vs. NH of example 44 +The removal effect of-N is very obvious.
The molybdenum-antimony spectrophotometry method is adopted to detect TP in the water body treated in the embodiment 4 and the control group 4, a graph of the removing effect of the phycomycete symbiont in the embodiment 4 and the pure activated sludge system in the control group 4 on TP in the aquaculture wastewater shown in fig. 7 is obtained, and as can be seen from fig. 7, after 1d of culture, compared with the activated sludge in the control group 4, the removing effect of the phycomycete symbiont system in the embodiment 4 on TP is very obvious. At time 2d, the TP level in the symbiotic system of the phycomycetes in example 4 was reduced to 0.
And (3) detecting sulfamethoxazole in the water body treated by the embodiment 4 and the control group 4 by adopting an HPLC method, wherein the detection conditions are as follows: agilent HPLC machine with mobile phase of 55% acetonitrile and 45% formic acid, C18 column, flow rate of 1mL/min, sulfamethoxazole peaking at 1.2 min.
As shown in FIG. 8, a graph of the removal effect of sulfamethoxazole in the aquaculture wastewater by the pure activated sludge system of the phycomycete symbiont of example 4 and the control group 4 is obtained, and as can be seen from FIG. 8, the removal effect of sulfamethoxazole by the phycomycete symbiont system of example 4 is very obvious compared with the activated sludge of the control group 4 after 5d of culture.
Example 6: the cultivation wastewater shown in Table 2 was treated with the algal bacteria symbiont obtained in example 4, which degrades cultivation wastewater containing high concentration antibiotics and having a low C/N ratio, and 1mg/L sulfamethoxazole was added to the cultivation wastewater, specifically, the treatment method was as follows:
the algal fungus symbiont of example 4 was inoculated at a dry weight of 500mg/L into sulfamethoxazole culture wastewater at a concentration of 1mg/L, and cultured at a culture temperature of 30 ℃ and a culture rotation speed of 200rpm under a culture illumination of 200. mu. mol/m2The treatment is carried out under the conditions that the aeration rate is 1.5L/min and the aerated gas is CO2And air, the mixed gas is CO2The mass fraction of the water is 2 percent, and the pH value of the water body is adjusted to be neutral every 12 hours from the beginning of inoculation.
The activated sludge culture system of the sewage treatment plant (Taiping sewage treatment plant in Harbin city) in the step 3 was used as a control group 5, and cultured under the same culture conditions as those of the example 7.
NH in the water body treated by the example 4 and the control group 5 by adopting an ultraviolet absorption method4 +Detection of-N to obtain NH in the aquaculture wastewater by the pure activated sludge system of the algal bacteria symbiont of example 4 and the control group 5 as shown in FIG. 94 +FIG. 9 shows NH pair of the symbiotic system of phycomycetes in example 4 after 5d cultivation compared with the activated sludge of control group 54 +The removal effect of-N is very obvious.
The molybdenum-antimony spectrophotometry method is adopted to detect the TP in the water body treated in the embodiment 4 and the control group 5, and a curve diagram of the removing effect of the phycomycete symbiont in the embodiment 4 and the pure activated sludge system in the control group 5 on the TP in the aquaculture wastewater shown in fig. 10 is obtained, and as can be seen from fig. 10, after the culture for 1d, compared with the activated sludge in the control group 5, the removing effect of the phycomycete symbiont system in the embodiment 4 on the TP is very obvious. At time 2d, the TP content in the phycobiont system of example 4 was substantially reduced to 0.
Claims (2)
1. The application of the phycomycete symbiont for degrading culture waste water containing high concentration antibiotic and low C/N ratio is characterized in that the phycomycete symbiont is used for treating the culture waste water containing high concentration antibiotic and low C/N ratio by the following method:
inoculating the phycomycete symbiont degrading the culture waste water containing high concentration antibiotic and low C/N ratio into the culture waste water to be treated, culturing at 29-32 deg.c and at 200-300 rpm under 150 μmol/m illumination2/s～300μmol/m2The treatment is carried out under the condition that the aeration quantity is 1L/min-2L/min, and the pH value of the water body is adjusted to be neutral every 12 hours from the beginning of inoculation; the antibiotic is sulfamethoxazole;
the preparation method of the phycomycete symbiont for degrading the culture wastewater containing high-concentration antibiotics and low C/N ratio comprises the following steps:
step 1: adding Chlorella pyrenoidosa into BG-11 culture medium, then pre-culturing in a photobioreactor, and culturing to logarithmic phase to obtain Chlorella liquid; in which the photobiological reaction is carried outThe parameters for pre-culture in the apparatus were: the culture temperature is 29-32 ℃, the culture rotating speed is 200-300 rpm, and the culture illumination is 150 mu mol/m2/s～300μmol/m2The aeration rate is 1L/min-2L/min; the aerated gas is CO2Mixed gas with air, CO in the mixed gas2The mass fraction of (a) is 2%;
step 2: centrifuging the chlorella solution obtained in the step 1, removing supernatant, washing the centrifuged chlorella solution with distilled water to obtain chlorella cell sediment, centrifuging again, removing supernatant to obtain chlorella cell sediment;
and step 3: continuously aerating activated sludge by using an air pump, and then domesticating by using artificial simulated culture wastewater to obtain an activated sludge suspension of a specific microbial community; the artificial simulated aquaculture wastewater is aquaculture wastewater containing antibiotics and having a low C/N ratio, and comprises the following specific components: 7.25. + -. 0.05, COD: 620 mg/L-650 mg/L, NO3 --N：40mg/L～45mg/L、NH4 +-N: 420 mg/L-450 mg/L, TP: 12mg/L to 16 mg/L; the microbial community in the activated sludge of the obtained specific microbial community comprises Proteobacteria, bacteroidata, Cyanobacteria, bdellovibroota, Firmicutes, Actinobacteriota, myxococca, acidobacteriacea, Verrucomicrobiota; the aerated gas is CO2Mixed gas with air, CO in mixed gas2The mass fraction of (A) is 2%;
and 4, step 4: centrifuging the activated sludge suspension of the specific microbial community obtained in the step 3 to remove supernatant, washing the centrifuged activated sludge precipitate with distilled water, centrifuging again to remove supernatant to obtain activated sludge precipitate;
and 5: and (3) uniformly mixing the chlorella cell precipitate obtained in the step (2) and the activated sludge precipitate obtained in the step (4) to obtain the chlorella symbiont for degrading the culture wastewater containing high-concentration antibiotics and having a low C/N ratio, wherein the mass ratio of the chlorella cell precipitate to the activated sludge precipitate in terms of dry weight of each chlorella cell precipitate is (1-10): 1.
2. The method according to claim 1 for degrading algal bacteria symbiont containing high concentration antibiotic and low C/N ratio cultivation waste waterThe method is characterized in that the dry weight of the chlorella in the chlorella liquid obtained in the step 1 in the step 2 is calculated by an OD linear relation equation which is as follows: the dry weight (g/L) of Chlorella in Chlorella solution is 0.2519 XOD680+0.0123，R2＝0.9972。
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