CN112320928A - Method for treating pickling wastewater by using activated sludge-dunaliella salina mixture - Google Patents
Method for treating pickling wastewater by using activated sludge-dunaliella salina mixture Download PDFInfo
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- 239000002351 wastewater Substances 0.000 title claims abstract description 74
- 238000005554 pickling Methods 0.000 title claims abstract description 37
- 238000000034 method Methods 0.000 title claims abstract description 34
- 241001466077 Salina Species 0.000 title claims abstract description 8
- 239000000203 mixture Substances 0.000 title claims description 5
- 241000195633 Dunaliella salina Species 0.000 claims abstract description 52
- 239000010802 sludge Substances 0.000 claims abstract description 39
- 238000009938 salting Methods 0.000 claims abstract description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 19
- 239000001963 growth medium Substances 0.000 claims description 40
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 23
- 241000195493 Cryptophyta Species 0.000 claims description 19
- 150000003839 salts Chemical class 0.000 claims description 14
- 239000011780 sodium chloride Substances 0.000 claims description 12
- 238000006243 chemical reaction Methods 0.000 claims description 10
- 238000002156 mixing Methods 0.000 claims description 9
- 238000001914 filtration Methods 0.000 claims description 8
- 239000008213 purified water Substances 0.000 claims description 8
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 claims description 5
- 229910021578 Iron(III) chloride Inorganic materials 0.000 claims description 5
- 229910052564 epsomite Inorganic materials 0.000 claims description 5
- XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 claims description 5
- RBTARNINKXHZNM-UHFFFAOYSA-K iron trichloride Chemical compound Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 claims description 5
- 239000002245 particle Substances 0.000 claims description 5
- 238000000746 purification Methods 0.000 claims description 4
- 239000012267 brine Substances 0.000 claims description 3
- HPALAKNZSZLMCH-UHFFFAOYSA-M sodium;chloride;hydrate Chemical compound O.[Na+].[Cl-] HPALAKNZSZLMCH-UHFFFAOYSA-M 0.000 claims description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract description 34
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 abstract description 18
- 229910052698 phosphorus Inorganic materials 0.000 abstract description 18
- 239000011574 phosphorus Substances 0.000 abstract description 18
- 229910052757 nitrogen Inorganic materials 0.000 abstract description 17
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 abstract description 13
- 239000010865 sewage Substances 0.000 abstract description 12
- 230000000694 effects Effects 0.000 abstract description 9
- 230000015556 catabolic process Effects 0.000 abstract description 5
- 238000006731 degradation reaction Methods 0.000 abstract description 5
- 238000005286 illumination Methods 0.000 abstract description 5
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract description 3
- 230000007613 environmental effect Effects 0.000 abstract description 3
- 239000010842 industrial wastewater Substances 0.000 abstract description 3
- 229910052760 oxygen Inorganic materials 0.000 abstract description 3
- 239000001301 oxygen Substances 0.000 abstract description 3
- 230000002195 synergetic effect Effects 0.000 abstract description 3
- 238000004065 wastewater treatment Methods 0.000 abstract description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 abstract description 2
- 229910052799 carbon Inorganic materials 0.000 abstract description 2
- 238000004134 energy conservation Methods 0.000 abstract description 2
- 235000002639 sodium chloride Nutrition 0.000 description 20
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 9
- 244000005700 microbiome Species 0.000 description 8
- 238000012258 culturing Methods 0.000 description 7
- 238000004062 sedimentation Methods 0.000 description 5
- 238000011081 inoculation Methods 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 230000006978 adaptation Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
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- 241000195627 Chlamydomonadales Species 0.000 description 1
- 241000196319 Chlorophyceae Species 0.000 description 1
- 241000195628 Chlorophyta Species 0.000 description 1
- 101710088194 Dehydrogenase Proteins 0.000 description 1
- 241000195634 Dunaliella Species 0.000 description 1
- 241000206602 Eukaryota Species 0.000 description 1
- 241000243820 Polychaeta Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000001112 coagulating effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
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- 231100000719 pollutant Toxicity 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 238000005185 salting out Methods 0.000 description 1
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- 238000005406 washing Methods 0.000 description 1
- 238000003911 water pollution Methods 0.000 description 1
Images
Classifications
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/02—Aerobic processes
- C02F3/12—Activated sludge processes
- C02F3/1205—Particular type of activated sludge processes
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
- C02F3/325—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae as symbiotic combination of algae and bacteria
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F9/00—Multistage treatment of water, waste water or sewage
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/001—Processes for the treatment of water whereby the filtration technique is of importance
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/66—Treatment of water, waste water, or sewage by neutralisation; pH adjustment
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/32—Nature of the water, waste water, sewage or sludge to be treated from the food or foodstuff industry, e.g. brewery waste waters
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Abstract
The invention relates to the technical field of industrial wastewater treatment, in particular to a method for treating pickling wastewater by using activated sludge-dunaliella salina. The invention utilizes the synergistic effect of the dunaliella salina and the activated sludge to effectively remove ammonia nitrogen, total phosphorus and COD in the sewage, and solves the problems that the COD in the water and the COD caused by the increase of the BOD are difficult to effectively remove when the salting waste water is treated by the single dunaliella salina, the effect of the single activated sludge on removing the total nitrogen and the total phosphorus in the salting waste water is not obvious, and the like. The method has obvious removal effect on ammonia nitrogen, total nitrogen and total phosphorus, COD and the like, does not need to additionally add oxygen supply equipment or add a carbon source, has low treatment cost, does not have the risk of secondary pollution, can recycle the dunaliella salina, has high economic value, and realizes resource utilization. The method can be carried out only under proper temperature and illumination conditions, and has the advantages of obvious degradation effect, simple operation, low operating cost, energy conservation, environmental protection and the like.
Description
Technical Field
The invention relates to the technical field of industrial wastewater treatment, in particular to a method for treating pickling wastewater by using activated sludge-dunaliella salina.
Background
With the rapid development of the pickled food industry, the food pickling process brings economic value and also generates high-salinity industrial wastewater, the wastewater mainly comes from pickling, elutriation and ground washing, and the common salt dehydration process in the pickling process generates a large amount of high-salinity wastewater. The wastewater contains a large amount of water-soluble organic matters and soluble inorganic salts, nitrogen and phosphorus organic matters in the wastewater are nutrient elements required by the growth of microorganisms, but the microorganisms are inhibited or even poisoned by overhigh concentration. How to efficiently treat high-salinity wastewater and complete resource utilization becomes a difficult problem to be solved urgently in current water pollution treatment.
At present, most of microorganisms applied in the conventional biological water treatment process adopted for treating high-salinity wastewater in China belong to non-halophilic microorganisms, the microorganisms can cause the activity of dehydrogenase to be reduced under the action of salting out, and under the condition of high salinity, the cells can lose water along with the increase of salinity, so that cell plasmolysis and microorganism disintegration are caused. The osmotic pressure caused by high salinity increases the inhibitory effect on microorganisms, making it difficult to perform the conventional activated sludge process normally.
The activated sludge process is one of the most advocated technologies in the industry at present and is the most common biological treatment technology. The activated sludge method is mostly used for treating domestic sewage with low load and low salinity, such as municipal sewage, and aims at solving the problems of unstable treatment effect, high energy consumption and the like of high-salinity wastewater, although a small amount of research is carried out on the treatment of high-salinity wastewater. Dunaliella salina (Dunaliella salina) belongs to Chlorophyta, Chlorophyceae, Volvocales, Polychaeta, and Dunaliella, and is the most salt-tolerant eukaryote known at present. The glycerol in the dunaliella salina cells is correspondingly synthesized or transformed along with the increase and decrease of the concentration of external salt.
At present, salt tolerance of microorganisms is improved by adopting a mode of salt tolerance domestication or adding halophilic bacteria aiming at high-salinity wastewater. The degradation of the pickling wastewater completely depends on the self characteristics of the dunaliella salina or halophilic bacteria, the total nitrogen and total phosphorus in the wastewater are mainly degraded, the COD is not obviously removed, and the degradation effect of the method on the wastewater is limited by certain external environmental conditions. CN108117225A, a method for treating high-salt-content wastewater, which needs to add salt-tolerant bacteria agents into the existing membrane bioreactor, and adopts the processes of coagulating sedimentation, biochemical treatment and the like by using a lime-soda softening method to realize the high-efficiency removal of pollutants such as COD (chemical oxygen demand), total nitrogen and the like.
Disclosure of Invention
In order to overcome the defects and problems in the prior art, the invention provides a method for treating pickling wastewater by using activated sludge-dunaliella salina. The method utilizes the synergistic effect of the dunaliella salina and the activated sludge, and overcomes the technical problems that COD is difficult to remove when the dunaliella salina is used for independently treating the pickling wastewater, and the treatment effect of the activated sludge on total phosphorus, total nitrogen and the like of the sewage is poor.
The technical scheme adopted by the invention is as follows:
the invention provides a method for treating pickling wastewater by using activated sludge-dunaliella salina mixture, which comprises the following steps:
(1) removing suspended particles after adjusting the pH of the pickling wastewater;
(2) putting the activated sludge into salting wastewater with different salt concentrations for gradient acclimation to obtain aerobic salt-tolerant activated sludge;
(3) putting the salt algae into salt algae culture media with different salt concentrations for gradient domestication to obtain salt-tolerant salt algae;
(4) mixing the aerobic salt-tolerant activated sludge obtained in the step (2) and the salt-tolerant dunaliella salina obtained in the step (3) into mud dunaliella salina, adding the mud dunaliella salina into the pickling wastewater to be treated for purification reaction, and filtering after the dunaliella salina grows to a stable period to obtain purified water.
The pH value in the step (1) is 6-8.
The domestication temperature in the step (2) is 20-30 ℃, and the salinity of the aerobic salt-tolerant activated sludge is 1-3%.
The dunaliella salina culture medium in the step (3) contains the following components in 1L: 1g C6H12O6，10.5g NaCl，0.1g KNO3，0.1g NaHCO3，0.01g CaCl2，0.1g MgSO4·7H2O，0.1g KCl，0.01g KH2PO4，0.001g Na2EDTA·2H2O and 0.001g FeCl3·6H2O。
And (4) the pH value of the brine alga culture medium in the step (3) is 6-8.
The volume ratio of the activated sludge to the dunaliella salina in the mud algae in the step (4) is 1-10: 1.
the inoculation amount of mud algae in the pickling wastewater to be treated in the step (4) is 25-35%.
The purification reaction in the step (4) is a reaction under natural light, the temperature is 20-30 ℃, and the time is 12-16 d.
Preferably, the salinity is gradually increased by 0.5% in the gradient acclimation.
Compared with the prior art, the invention has the beneficial effects that:
the invention utilizes the synergistic effect of the dunaliella salina and the activated sludge to effectively remove ammonia nitrogen, total phosphorus and COD in the sewage, and solves the problems that the COD in the water and the COD caused by the increase of the BOD are difficult to effectively remove when the salting waste water is treated by the single dunaliella salina, the effect of the single activated sludge on removing the total nitrogen and the total phosphorus in the salting waste water is not obvious, and the like. Due to the salt resistance of the dunaliella salina and the salt-tolerant activated sludge, the degradation treatment of the high-salinity wastewater is not limited by the salt concentration in the wastewater, the ammonia nitrogen, the total nitrogen and the total phosphorus, the COD and the like are obviously removed, oxygen supply equipment does not need to be additionally added, a carbon source does not need to be added, the treatment cost is low, the risk of secondary pollution does not exist, the dunaliella salina can be recycled, the economic value is high, and the resource utilization is realized. The method can be carried out only under proper temperature and illumination conditions, and has the advantages of obvious degradation effect, simple operation, low operating cost, energy conservation, environmental protection and the like.
Drawings
FIG. 1 is a flow chart of the pickling wastewater treatment by activated sludge-dunaliella salina mixture.
Detailed Description
Unless otherwise specified, the reagents involved in the examples of the present invention are all commercially available products, and all of them are commercially available.
In the following specific examples, the pickling wastewater used was simulated by manually distributing water and the activated sludge used was obtained from a secondary sedimentation tank of a sewage plant.
Example 1
(1) Pretreatment of pickling wastewater: adjusting the pH value of the pickling wastewater to 8.0 by NaOH in an adjusting tank, and filtering to remove suspended particles so as to improve the turbidity of a water body; the salinity of the pretreated pickling wastewater is 10.2g/L, the total phosphorus concentration is 25.4 mg/L, the total nitrogen concentration is 277.5mg/L, the ammonia nitrogen concentration is 82.73mg/L, and the COD is 2515 mg/L;
(2) domesticating activated sludge: inoculating activated sludge in a secondary sedimentation tank of a certain sewage plant, and acclimating the activated sludge into salt-tolerant activated sludge with the salinity of about 1% (based on NaCl) by a gradual acclimation method; the gradual domestication process is carried out in an SBR reactor, the salting waste water amount pretreated in the adding step (1) is controlled, the salinity of the inlet water is increased by 0.5 percent (based on NaCl) each time, the gradual domestication is carried out until the salting waste water is added according to the proportion of 100 percent, and the aerobic salt-tolerant activated sludge with the salinity of about 1 percent is finally domesticated;
(3) domesticating the dunaliella salina: the dunaliella salina culture medium comprises the following components in 1L: 1g C6H12O6，10.5g NaCl，0.1g KNO3，0.1g NaHCO3，0.01g CaCl2，0.1g MgSO4·7H2O，0.1g KCl，0.01g KH2PO4，0.001g Na2EDTA·2H2O and 0.001g FeCl3·6H2O; the pH value of the dunaliella salina culture medium is 8. Mixing a dunaliella salina culture medium with the pretreated pickling wastewater with the volume percentage concentration of 30% to obtain a domestication culture medium A, and mixing the dunaliella salina culture medium with the pretreated pickling wastewater with the volume percentage concentration of 80% to obtain a domestication culture medium B; inoculating the dunaliella salina into an acclimation culture medium A, culturing well, transferring to an acclimation culture medium B for continuous culture, and repeatedly culturing in the culture medium A and the culture medium B until the dunaliella salina is transferred into the acclimation culture medium containing 100% of pretreated pickling wastewater and can normally grow to obtain the acclimated dunaliella salina;
(4) constructing an activated sludge-dunaliella symbiotic system: mixing the aerobic salt-tolerant activated sludge obtained in the step (2) and the domesticated dunaliella salina obtained in the step (3) in a volume ratio of 10:1 to obtain mud algae (the activated sludge is calculated by SV30, the dunaliella salina is 2.1-107 cells/ml), and adding the mud algae into the to-be-treated salting wastewater of a biological reaction tank, wherein the total inoculation amount of the mud algae is 25% (v/v). Setting the reaction condition as 20 ℃, stirring and reacting for 12d under the illumination of natural light, and after the dunaliella salina grows to a stable period, precipitating and filtering the salting waste water to obtain purified water. The detection proves that the total phosphorus removal rate of the curing wastewater in the embodiment is 93.1%, the total nitrogen removal rate is 83.2%, the ammonia nitrogen removal rate is 91.1%, and the COD removal rate is 90.1%. The indexes of COD, ammonia nitrogen, total phosphorus, total nitrogen and the like in the purified water reach the second-level standard of the comprehensive sewage discharge standard.
Example 2
(1) Pretreatment of pickling wastewater: adjusting the pH value of the pickling wastewater to 7.0 by using NaOH, and filtering to remove suspended particles so as to improve the turbidity of the water body; the salinity of the pretreated pickling wastewater is 25.8g/L, the total phosphorus concentration is 27.9mg/L, the total nitrogen concentration is 281.5mg/L, the ammonia nitrogen concentration is 87.52mg/L, and the COD is 2542 mg/L;
(2) inoculating activated sludge in a secondary sedimentation tank of a certain sewage plant, and acclimating the activated sludge into salt-tolerant activated sludge with the salinity of about 2.5% (calculated by NaCl) by a gradual acclimation method; and (2) performing gradual domestication in an SBR reactor, and gradually domesticating by controlling the amount of the salting wastewater pretreated in the adding step (1) and increasing the salinity of the inlet water by 0.5 percent (based on NaCl) each time until the salting wastewater is added according to a proportion of 100 percent, and finally domesticating the aerobic salt-tolerant activated sludge with the salinity of about 2.5 percent.
(3) Domesticating the dunaliella salina: the dunaliella salina culture medium comprises the following components in 1L: 5g C6H12O6，25g NaCl，0.5g KNO3，0.5g NaHCO3，0.05g CaCl2，0.5g MgSO4·7H2O，0.5g KCl，0.05g KH2PO4，0.005g Na2EDTA· 2H2O and 0.005g FeCl3· 6H2O, the pH value of the dunaliella salina culture medium is 7; mixing a dunaliella salina culture medium with pretreated pickling wastewater with volume percentage concentration of 40% to obtain a domesticated culture medium C, mixing the dunaliella salina culture medium with pretreated pickling wastewater with volume percentage concentration of 60% to obtain a domesticated culture medium D, inoculating the dunaliella salina into the domesticated culture medium C, culturing well, transferring to the domesticated culture medium D for continuous culture, and repeating in the culture medium C and the culture medium D in sequence until the dunaliella salina is transferred into the domesticated culture medium containing 100% pretreated pickling wastewater and can normally growObtaining domesticated dunaliella salina;
(4) constructing an activated sludge-dunaliella symbiotic system: mixing the aerobic salt-tolerant activated sludge obtained in the step (2) and the domesticated dunaliella salina obtained in the step (3) in a volume ratio of 2:1 to obtain mud algae, and adding the mud algae into the pickling wastewater to be treated, wherein the total inoculation amount of the mud algae is 30% (v/v). Setting the reaction condition as 25 ℃, stirring and reacting for 14d under the illumination of natural light, and filtering the salting wastewater to obtain purified water after the dunaliella salina grows to a stable period. Through determination, the total phosphorus removal rate, the total nitrogen removal rate and the ammonia nitrogen removal rate of the curing wastewater in the embodiment are respectively 96.5%, 86.3%, 95.3% and 92.1%. The indexes of COD, ammonia nitrogen, total phosphorus, total nitrogen and the like in the purified water reach the second-level standard of the comprehensive sewage discharge standard.
Example 3
(1) Pretreating pickling wastewater: adjusting the pH value of the pickling wastewater to 6.0 by using NaOH, and filtering to remove suspended particles so as to improve the turbidity of the water body; the salinity of the pretreated pickling wastewater is 33.4g/L, the total phosphorus concentration is 29.2mg/L, the total nitrogen concentration is 293.4mg/L, the ammonia nitrogen concentration is 89.6mg/L, and the COD is 2577 mg/L;
(2) domesticating activated sludge: inoculating activated sludge in a secondary sedimentation tank of a certain sewage plant, and acclimating the activated sludge into salt-tolerant activated sludge with the salinity of about 3% (based on NaCl) by a gradual acclimation method; the gradual domestication process is carried out in an SBR reactor, the salting waste water amount pretreated in the adding step (1) is controlled, the salinity of the inlet water is increased by 0.5 percent (based on NaCl) each time, the gradual domestication is carried out until the salting waste water is added according to the proportion of 100 percent, and the aerobic salt-tolerant activated sludge with the salinity of about 3 percent is finally domesticated;
(3) domesticating the dunaliella salina: the dunaliella salina culture medium comprises the following components in 1L: 10g C6H12O6，30g NaCl，1g KNO3，1g NaHCO3，0.1g CaCl2，1g MgSO4·7H2O，1g KCl，0.1g KH2PO4，0.01g Na2EDTA· 2H2O and 0.01g FeCl3· 6H2O, the pH value of the dunaliella salina culture medium is 6. Inoculating Dunaliella salina into acclimatization culture medium A, culturing, transferring to acclimatization culture medium after good growthContinuously culturing in the culture medium D, transferring to a domestication culture medium B for culturing after the culture medium D grows well, and sequentially and repeatedly culturing in the culture medium A, the culture medium D and the culture medium B until the culture medium is transferred to the domestication culture medium containing 100% of pretreated salting wastewater and can normally grow to obtain domesticated dunaliella salina;
(4) constructing an activated sludge-dunaliella symbiotic system: mixing the aerobic salt-tolerant activated sludge obtained in the step (2) and the domesticated dunaliella salina obtained in the step (3) in a volume ratio of 1:1 to obtain mud algae, and adding the mud algae into the pickling wastewater to be treated, wherein the total inoculation amount of the mud algae is 35% (v/v). Setting the corresponding growth conditions, the temperature is 30 ℃, the illumination is natural light, the reaction time is 16d, and the reaction is carried out under the condition of stirring. And when the dunaliella salina grows to a stable period, filtering the salting waste water to obtain purified water. Through determination, the total phosphorus removal rate, the total nitrogen removal rate, the ammonia nitrogen removal rate and the COD removal rate of the curing wastewater in the embodiment are respectively 95.2%, 84.9%, 93.6% and 91.2%. The indexes of COD, ammonia nitrogen, total phosphorus, total nitrogen and the like in the purified water reach the second-level standard of the comprehensive sewage discharge standard.
The above is only a preferred embodiment of the present invention, and it should be noted that the above preferred embodiment should not be considered as limiting the present invention, and the protection scope of the present invention should be subject to the scope defined by the claims. It will be apparent to those skilled in the art that various modifications and adaptations can be made without departing from the spirit and scope of the invention, and these modifications and adaptations should be considered within the scope of the invention.
Claims (8)
1. A method for treating pickling wastewater by using activated sludge-dunaliella salina mixture is characterized by comprising the following steps:
(1) removing suspended particles after adjusting the pH of the pickling wastewater;
(2) putting the activated sludge into salting wastewater with different salt concentrations for gradient acclimation to obtain aerobic salt-tolerant activated sludge;
(3) putting the salt algae into salt algae culture media with different salt concentrations for gradient domestication to obtain salt-tolerant salt algae;
(4) mixing the aerobic salt-tolerant activated sludge obtained in the step (2) and the salt-tolerant dunaliella salina obtained in the step (3) into mud dunaliella salina, adding the mud dunaliella salina into the pickling wastewater to be treated for purification reaction, and filtering after the dunaliella salina grows to a stable period to obtain purified water.
2. The method of treating salting waste water according to claim 1, wherein the pH in the step (1) is 6 to 8.
3. The method for treating salting wastewater according to claim 1, wherein the gradient acclimation temperature in the step (2) is 20-30 ℃, and the salinity of the aerobic salt-tolerant activated sludge is 1% -3%.
4. The method of treating pickling wastewater as set forth in claim 1, wherein the culture medium for brine alga in the step (3) comprises the following components in 1L: 1gC6H12O6，10.5g NaCl，0.1g KNO3，0.1g NaHCO3，0.01g CaCl2，0.1g MgSO4·7H2O，0.1g KCl，0.01g KH2PO4，0.001g Na2EDTA·2H2O and 0.001g FeCl3·6H2O。
5. The method for treating salting waste water according to claim 1, wherein the pH of the brine alga culture medium in the step (3) is 6 to 8.
6. The method for treating salting wastewater according to claim 1, wherein the volume ratio of activated sludge to dunaliella salina in the mud algae in the step (4) is 1-10: 1.
7. the method for treating salting waste water according to claim 1, wherein the amount of the mud algae inoculated into the salting waste water to be treated in the step (4) is 25 to 35%.
8. The method for treating salting waste water according to claim 1, wherein the purification reaction in the step (4) is a reaction under natural light at a temperature of 20 to 30 ℃ for 12 to 16 days.
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| CN109880745A (en) * | 2019-03-15 | 2019-06-14 | 江苏大学 | A method of using pickling waste water, shining bittern water subsection filter salt algae |
| CN113292164A (en) * | 2021-04-30 | 2021-08-24 | 哈尔滨工业大学 | Preparation method and application of phycomycete symbiont for degrading culture wastewater containing high-concentration antibiotics and having low C/N ratio |
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| CN104609542A (en) * | 2013-11-05 | 2015-05-13 | 中蓝连海设计研究院 | Method for culturing salt-tolerant aerobic activated sludge |
| CN109928505A (en) * | 2019-03-15 | 2019-06-25 | 江苏大学 | A method of pickling waste water is handled using salt algae, Halophiles |
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| CN102849857A (en) * | 2012-04-10 | 2013-01-02 | 南京大学 | Method for treating high-salinity high-concentration ammonia-nitrogen wastewater |
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| CN109880745A (en) * | 2019-03-15 | 2019-06-14 | 江苏大学 | A method of using pickling waste water, shining bittern water subsection filter salt algae |
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