CN109928505A - A method of pickling waste water is handled using salt algae, Halophiles - Google Patents
A method of pickling waste water is handled using salt algae, Halophiles Download PDFInfo
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- CN109928505A CN109928505A CN201910198133.7A CN201910198133A CN109928505A CN 109928505 A CN109928505 A CN 109928505A CN 201910198133 A CN201910198133 A CN 201910198133A CN 109928505 A CN109928505 A CN 109928505A
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Abstract
The present invention relates to industrial waste water treatment more particularly to a kind of methods for utilizing salt algae, Halophiles to handle pickling waste water.The method of the invention includes the pretreatment of pickling waste water, the domestication of culture medium gradient, building helotism system and collects bacterium algae；The method of the invention can effectively remove nitrogen in pickling waste water, phosphorus and total nitrogen content, not restricted by salinity in waste water in degradation treatment high-salt wastewater, have preferable Nitrogen/Phosphorus Removal to pickling waste water；It is not limited and additional oxygen supply equipment by no anoxic or aerobic condition, also not against additional carbon；Only need to matters illumination and at a temperature of, that is, can reach good degradation effect；Have many advantages, such as that operating cost is low, it is environmentally protective.
Description
Technical field
The present invention relates to industrial waste water treatments more particularly to a kind of utilization salt algae, Halophiles processing to pickle and give up
The method of water.
Background technique
The waste water that marinated food generates in the process of production and processing mainly from elutriation, squeezing, marinated and ground flushing,
Common dehydration of salt technique can generate a large amount of high bittern water, also, suspended matter, total nitrogen, ammonia in pickling waste water in curing process
Nitrogen, total phosphorus concentration etc. severely exceed.
Currently, the microorganism used in standard biologic water treatment technology largely belongs to non-halophilic microorganism, with salt
The activity of the increase of the degree quasi-microorganism is gradually reduced.The dehydrogenase activity of microorganism can drastically reduce under high salinity, micro- life
The osmotic pressure of the intracellular water of object increases with the raising of salinity, is dehydrated so as to cause microbial cell and then causes cell former
Raw matter separation, it is dead to eventually lead to microbial cell rupture.
Other processing methods of high salinity industrial wastewater have evaporation, incineration method, electrochemical process, advanced oxidation processes, absorption
Method etc..It is compared with traditional bioanalysis, above method capital cost and operating cost are all costly and there are secondary pollutions
Hidden danger, the defect with " with high investment, high energy consumption, low return ".How to deal carefully with high-salt wastewater and complete resource utilization at
For a project of assaulting fortified position of current water pollution control.
Dunaliella salina (Dunaliella salina) is under the jurisdiction of Chlorophyta, Chlorophyceae, volvocales, crinosity algae section, Du Shi algae
Belong to, is to be currently known the strongest eucaryote of salt tolerance.Glycerol in Dunaliella salina cell is corresponding with the increase and decrease of extraneous salinity
Synthesis or conversion.Halophiles refers to a kind of bacterium that growth metabolism can be just normally carried out under high salt conditions, is distributed mainly on salt
In the hypersaline environments such as lake, salt alkali lake, sabkha and saltern, slight thermophilic salt can be divided into according to the difference of the most suitable salt concentration range of microorganism
Bacterium, Halophilic Bacterium and Natrinema altunense sp.
Currently, improving the salt tolerant of microorganism for the mode that high-salt wastewater generallys use salt tolerant domestication or adds Halophiles
Performance.In the prior art, CN201810287752, a kind of biology-materialization joint technology handle the side of pickles cleaning waste water with high salt
Method.This method is handled by the way of salt tolerant acclimated activated sludge in biological treatment stage, and needs to add coagulation, alkalinity
The techniques such as stripping, activated carbon adsorption improve the effect of denitrogenation dephosphorizing, and in addition this method has the salt content of pickling waste water certain
Limitation.CN200910095742, Halophiles cultural method and the side using the cultivated Halophiles processing pickling waste water of this method
Method.This method first tames out the Halophiles for adapting to pickling waste water, then adds marinated useless to anaerobism, aerobic stage combined degradation
Water, but Halophiles characteristic is fully relied on to the degradation of pickling waste water, and need Anoxic/Aerobic segmentation degradation, the degradation of such method
Effect is subject to certain restrictions.
Summary of the invention
In order to overcome the above-mentioned deficiency handled in high-salt wastewater method in the prior art, the present invention provides a kind of using salt
The method that algae, Halophiles handle pickling waste water, the method are not restricted by waste water salinity, are obtained while degrading waste water
High value by-product realizes the purpose of resource utilization pickling waste water.
In order to solve the above technical problems, the technical solution of the present invention is as follows: a kind of handle pickling waste water using salt algae, Halophiles
Method, comprising the following steps:
(1) pretreatment of pickling waste water: filtering clarification after pickling waste water is adjusted pH, spare；
(2) culture medium gradient is tamed: configuration bacterium algae culture medium, by pretreatment in (1) the step of bacterium algae culture medium and various concentration
Pickling waste water mixing afterwards, obtains the domestication culture medium of various concentration, and inoculation Halophiles, salt algae carry out ladder into domestication culture medium
Degree domestication culture, Halophiles, salt algae after being tamed；
(3) it constructs helotism system: being inoculated into after the Halophiles after domestication in step (2), salt algae are mixed in a certain ratio
In step (1) treated pickling waste water, Suitable sites are set, handle pickling waste water；
(4) it collects bacterium algae: bacterium algae is collected by centrifugation after bacterium algae grows to stationary phase, completes the processing of pickling waste water.
Preferably, present invention adjusting pH as described in step (1) is to adjust pH to 6-8.
Preferably, bacterium algae culture medium described in step (2) of the present invention contains following component: 1-10g/L
C6H12O6, 100-250 g/L NaCl, 0.1-1 g/L KNO3, 0.1-1 g/L NaHCO3, 0.01-0.1 g/L CaCl2,
0.1-1 g/L MgSO4·7H2O, 0.1-1 g/L KCl, 0.01-0.1 g/L KH2PO4, 0.001-0.01 g/L
Na2EDTA·2H2O and 0.001-0.01 g/L FeCl3·6H2O, pH 6-8.
Preferably, the domestication of gradient described in step (2) of the present invention culture is lower dense to be first seeded to Halophiles, salt algae
In the domestication culture medium of degree, in the domestication culture medium that is forwarded to higher concentration again after Halophiles, dunaiella salina growth are good, successively follow
Ring is until Halophiles, salt algae normal growth in pickling waste water.
Preferably, the mixing of certain proportion described in step (3) of the present invention refers to Halophiles, salt algae with initial cell density 1:
The ratio of 0.1-10cell/mL mixes.
Preferably, inoculation described in step (3) refers to adds according to the volume ratio of step (1) treated pickling waste water 5-15 %
Add Halophiles, salt algae mixing total amount.
Preferably, Suitable sites described in step (3) are as follows: 20-30 DEG C of temperature, intensity of illumination 4000-6000 Lux, Quan Guang
According to.
Preferably, centrifugation described in step (4) are as follows: 4000-6000 r/min is centrifuged 10-20 min.
Compared with prior art, the beneficial effects of the present invention are:
Method provided by the present invention using salt algae, Halophiles processing pickling waste water can be removed effectively in pickling waste water
Nitrogen, phosphorus and total nitrogen content, due to salt algae and the unique physiological property of Halophiles, in degradation treatment high-salt wastewater not by waste water
The restriction of salinity has preferable Nitrogen/Phosphorus Removal to pickling waste water.Moreover, do not limited by no anoxic or aerobic condition and
Additional oxygen supply equipment, also not against additional carbon；Only need to matters illumination and at a temperature of, that is, can reach good degradation effect；
Have many advantages, such as that operating cost is low, it is environmentally protective.
The present invention, which degrades while high-salt wastewater purifies water, can harvest high yield bacterium algae, complete eco-energy self-loopa, thermophilic
Salt bacterium, salt algae can be used as bio-feritlizer and additive, rich in multiple beneficials such as protein, total rouge, beta carotene, total reducing sugars
Substance, the substances such as salt, nitrogen, carbon, phosphorus in resource utilization waste water.
Detailed description of the invention
Fig. 1 is the Technology Roadmap that pickling waste water nitrogen phosphorus is removed using salt algae-Halophiles.
Specific embodiment
With reference to the accompanying drawing 1 and specific embodiment the present invention is further described, but protection scope of the present invention is not
It is limited to this.Unless otherwise specified, reagent involved in the embodiment of the present invention is commercial product, can pass through commercial channel
Purchase obtains.
Embodiment 1:
Clarification is filtered after adjusting the pH to 8.0 of pickling waste water, suspended particulate substance is removed, improves the turbidity of water body；It gives up after pretreatment
The salinity of water is 106 g/L, and total phosphorus concentration is 76.15 mg/L, and total nitrogen concentration is 638.24 mg/L, ammonia nitrogen concentration 153.47
mg/L；
Bacterium algae culture medium is configured, following component: 1 g C is contained in terms of 1 L6H12O6, 100 g NaCl, 0.1 g KNO3, 0.1 g
NaHCO3, 0.01 g CaCl2, 0.1 g MgSO4·7H2O, 0.1 g KCl, 0.01 g KH2PO4, 0.001 g Na2EDTA·
2H2O and 0.001 g FeCl3·6H2O, bacterium algae medium pH are 8；By the pre- of bacterium algae culture medium and concentration of volume percent 30%
Treated, and pickling waste water is mixed to get domestication culture medium A, after the pretreatment of bacterium algae culture medium and concentration of volume percent 80%
Pickling waste water be mixed to get domestication culture medium B, by Halophiles, salt algae be seeded in domestication culture medium A in cultivate well-grown after
It is transferred in domestication culture medium B and continues to cultivate, be repeated in until being forwarded to the pretreated pickling waste water containing 100%
Taming can Halophiles, salt algae with normal growth, after being tamed in culture medium.
By after domestication Halophiles, salt algae in initial cell density 1:10cell/mL ratio mix, be added to pretreatment
In pickling waste water afterwards, the total inoculum concentration for mixing bacterium algae is 5% (v/v).Then setting Suitable sites are as follows: 20 DEG C of temperature, illumination is strong
Spend 4000 Lux, full exposure；After Halophiles, dunaiella salina growth to stationary phase in pickling waste water, 4000 r/min are centrifuged 10 min
Collect Halophiles, salt algae.After measured, the total tp removal rate of pickling waste water is 93% in the present embodiment, nitrogen removal rate 74%, ammonia nitrogen
Removal rate is 91%.The total candy output of bacterium algae is 0.73 g/L, and protein output is 0.87 g/L.
Embodiment 2:
Clarification is filtered after adjusting the pH to 7.0 of pickling waste water, suspended particulate substance is removed, improves the turbidity of water body；It gives up after pretreatment
The salinity of water is 182 g/L, and total phosphorus concentration is 79.97 mg/L, total nitrogen concentration 647.79mg/L, and ammonia nitrogen concentration is
186.98mg/L。
Bacterium algae culture medium is configured, following component: 5 g C is contained in terms of 1 L6H12O6, 180 g NaCl, 0.5 g KNO3,
0.5 g NaHCO3, 0.05 g CaCl2, 0.5 g MgSO4·7H2O, 0.5 g KCl, 0.05 g KH2PO4, 0.005 g
Na2EDTA·2H2O and 0.005 g FeCl3·6H2O, bacterium algae medium pH are 7；Bacterium algae culture medium and percent by volume is dense
The pretreated pickling waste water of degree 40% is mixed to get domestication culture medium C, by bacterium algae culture medium and concentration of volume percent 60%
Pretreated pickling waste water be mixed to get domestication culture medium D, by Halophiles, salt algae be seeded in domestication culture medium C in cultivate
Be transferred to after well-grown domestication culture medium D in continue to cultivate, be repeated in until be forwarded to containing 100% it is pretreated
It can Halophiles, salt algae with normal growth, after being tamed in the domestication culture medium of pickling waste water.
By after domestication Halophiles, salt algae in initial cell density 1:1cell/mL ratio mix, be added to pretreatment
In pickling waste water afterwards, the total inoculum concentration for mixing bacterium algae is 10% (v/v).Then setting Suitable sites are as follows: 25 DEG C of temperature, illumination
4000 Lux of intensity, full exposure；After Halophiles, dunaiella salina growth to stationary phase in pickling waste water, 6000 r/min centrifugation 20
Min collects Halophiles, salt algae.After measured, the total tp removal rate of pickling waste water is 94% in the present embodiment, nitrogen removal rate 76%,
Ammonia nitrogen removal frank is 92%.The total candy output of bacterium algae is 0.86 g/L, and protein output is 0.91 g/L.
Embodiment 3:
Clarification is filtered after adjusting the pH to 6.0 of pickling waste water, suspended particulate substance is removed, improves the turbidity of water body；It gives up after pretreatment
The salinity of water is 243 g/L, and total phosphorus concentration is 83.15 mg/L, and total nitrogen concentration is 657.98 mg/L, ammonia nitrogen concentration 196.53
mg/L。
Bacterium algae culture medium is configured, following component: 10 g C is contained in terms of 1 L6H12O6, 250 g NaCl, 1 g KNO3, 1 g
NaHCO3, 0.1 g CaCl2, 1 g MgSO4·7H2O, 1 g KCl, 0.1 g KH2PO4, 0.01 g Na2EDTA·2H2O and
0.01 g FeCl3·6H2O, bacterium algae medium pH are 6；By the pretreated of bacterium algae culture medium and concentration of volume percent 30%
Pickling waste water is mixed to get domestication culture medium A, and pretreated pickle of bacterium algae culture medium and concentration of volume percent 60% is given up
Water is mixed to get domestication culture medium D, and bacterium algae culture medium is mixed with the pretreated pickling waste water of concentration of volume percent 80%
Domestication culture medium B is obtained, Halophiles, salt algae is seeded in domestication culture medium A after cultivating well-grown and is transferred to domestication culture
Continue to cultivate in base D, be transferred to after well-grown domestication culture medium B in continue to cultivate, be repeated in until be forwarded to containing
It can Halophiles, salt algae with normal growth, after being tamed in the domestication culture medium of 100% pretreated pickling waste water.
By after domestication Halophiles, salt algae in initial cell density 1:0.1 cell/mL ratio mix, be added to pre- place
In pickling waste water after reason, the total inoculum concentration for mixing bacterium algae is 15% (v/v).Then setting Suitable sites are as follows: 30 DEG C of temperature, light
According to 6000 Lux of intensity, full exposure；After Halophiles, dunaiella salina growth to stationary phase in pickling waste water, 6000 r/min centrifugation 20
Min collects Halophiles, salt algae.After measured, the total tp removal rate of pickling waste water is 92% in the present embodiment, nitrogen removal rate 73%,
Ammonia nitrogen removal frank is 90%.The total candy output of bacterium algae is 0.93 g/L, and protein output is 0.98 g/L.
The above is only the preferred embodiment of the present invention, it is noted that above-mentioned preferred embodiment is not construed as pair
Limitation of the invention, protection scope of the present invention should be defined by the scope defined by the claims..For the art
For those of ordinary skill, without departing from the spirit and scope of the present invention, several improvements and modifications can also be made, these change
It also should be regarded as protection scope of the present invention into retouching.
Claims (8)
1. a kind of utilize salt algae, the method for Halophiles processing pickling waste water, which comprises the following steps:
(1) pretreatment of pickling waste water: filtering clarification after pickling waste water is adjusted pH, spare；
(2) culture medium gradient is tamed: configuration bacterium algae culture medium, by pretreatment in (1) the step of bacterium algae culture medium and various concentration
Pickling waste water mixing afterwards, obtains the domestication culture medium of various concentration, and inoculation Halophiles, salt algae carry out ladder into domestication culture medium
Degree domestication culture, Halophiles, salt algae after being tamed；
(3) it constructs helotism system: being inoculated into after the Halophiles after domestication in step (2), salt algae are mixed in a certain ratio
In step (1) treated pickling waste water, Suitable sites are set, handle pickling waste water；
(4) it collects bacterium algae: bacterium algae is collected by centrifugation after bacterium algae grows to stationary phase, completes the processing of pickling waste water.
2. according to claim 1 utilize salt algae, the method for Halophiles processing pickling waste water, which is characterized in that step (1)
Described in adjusting pH be adjust pH to 6-8.
3. according to claim 1 utilize salt algae, the method for Halophiles processing pickling waste water, which is characterized in that step (2)
Described in bacterium algae culture medium contain following component: 1-10g/L C6H12O6, 100-250 g/L NaCl, 0.1-1 g/L KNO3,
0.1-1 g/L NaHCO3, 0.01-0.1 g/L CaCl2, 0.1-1 g/L MgSO4·7H2O, 0.1-1 g/L KCl, 0.01-
0.1 g/L KH2PO4, 0.001-0.01 g/L Na2EDTA·2H2O and 0.001-0.01 g/L FeCl3·6H2O, pH 6-
8。
4. according to claim 1 utilize salt algae, the method for Halophiles processing pickling waste water, which is characterized in that step (2)
Described in gradient domestication culture for first Halophiles, salt algae are seeded in the domestication culture medium of low concentration, to Halophiles, salt
It is forwarded in the domestication culture medium of higher concentration, circuits sequentially until Halophiles, salt algae are in pickling waste water again after algae well-grown
Middle normal growth.
5. according to claim 1 utilize salt algae, the method for Halophiles processing pickling waste water, which is characterized in that step (3)
The certain proportion mixing refers to that Halophiles, salt algae are mixed with the ratio of initial cell density 1:0.1-10 cell/mL.
6. according to claim 1 utilize salt algae, the method for Halophiles processing pickling waste water, which is characterized in that step (3)
The inoculation, which refers to, adds Halophiles, salt algae combined amount according to the volume ratio of step (1) treated pickling waste water 5-15%.
7. according to claim 1 utilize salt algae, the method for Halophiles processing pickling waste water, which is characterized in that step (3)
The Suitable sites are as follows: 20-30 DEG C of temperature, intensity of illumination 4000-6000 Lux, full exposure.
8. according to claim 1 utilize salt algae, the method for Halophiles processing pickling waste water, which is characterized in that step (4)
The centrifugation are as follows: 4000-6000 r/min is centrifuged 10-20 min.
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| CN109880745A (en) * | 2019-03-15 | 2019-06-14 | 江苏大学 | A method of using pickling waste water, shining bittern water subsection filter salt algae |
| CN111747534A (en) * | 2020-07-27 | 2020-10-09 | 南昌航空大学 | Method for treating landfill leachate |
| CN112320928A (en) * | 2020-09-17 | 2021-02-05 | 江苏大学 | Method for treating pickling wastewater by using activated sludge-dunaliella salina mixture |
| CN112322499A (en) * | 2020-10-29 | 2021-02-05 | 深圳市鑫承诺环保产业股份有限公司 | Autotrophic heterotrophic alternative domestication cultured dunaliella salina and application thereof in treatment of marine ship domestic sewage |
| CN113151247A (en) * | 2021-03-04 | 2021-07-23 | 江苏大学 | Dunaliella salina-halophilic bacteria immobilized microsphere and preparation method and application thereof |
| CN113493810A (en) * | 2021-07-08 | 2021-10-12 | 深圳大学 | Super selenium-rich dunaliella salina product and preparation method and application thereof |
| CN114317276A (en) * | 2022-01-18 | 2022-04-12 | 山东海之宝海洋科技有限公司 | Dunaliella salina strain for treating salt-containing wastewater, microbial community comprising same and application thereof |
| CN114394677A (en) * | 2022-01-18 | 2022-04-26 | 山东海之宝海洋科技有限公司 | Method for treating salt-containing wastewater |
| CN118373525A (en) * | 2024-06-21 | 2024-07-23 | 山东润德生物科技有限公司 | Method for treating waste water from glucosamine production by biological method |
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| CN113151247B (en) * | 2021-03-04 | 2024-08-13 | 江苏大学 | Immobilized microsphere of brine alga-halophila, preparation method and application thereof |
| CN113493810A (en) * | 2021-07-08 | 2021-10-12 | 深圳大学 | Super selenium-rich dunaliella salina product and preparation method and application thereof |
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| CN114394677A (en) * | 2022-01-18 | 2022-04-26 | 山东海之宝海洋科技有限公司 | Method for treating salt-containing wastewater |
| CN114317276B (en) * | 2022-01-18 | 2024-07-23 | 山东海之宝海洋科技有限公司 | Dunaliella salina strain for treating saline wastewater, microbial community comprising same and application thereof |
| CN118373525A (en) * | 2024-06-21 | 2024-07-23 | 山东润德生物科技有限公司 | Method for treating waste water from glucosamine production by biological method |
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