CN109880745A - A method of using pickling waste water, shining bittern water subsection filter salt algae - Google Patents
A method of using pickling waste water, shining bittern water subsection filter salt algae Download PDFInfo
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- CN109880745A CN109880745A CN201910197758.1A CN201910197758A CN109880745A CN 109880745 A CN109880745 A CN 109880745A CN 201910197758 A CN201910197758 A CN 201910197758A CN 109880745 A CN109880745 A CN 109880745A
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- salt algae
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Abstract
The present invention relates to salt algae cultural technique fields, and in particular to a method of using pickling waste water, shine bittern water subsection filter salt algae.The present invention makes full use of the nutrients culture salt algaes such as pickling waste water, the sodium chloride in solarization bittern water and nitrogen phosphorus according to dunaiella salina growth characteristic；High yield salt algae is first obtained using enrichment culture mode, then promote the accumulation of by-product in salt frond in high salinity Fiber differentiation in brine of evaporating brine, the yield of the multi-functional by-product such as total rouge, beta carotene in salt frond is also improved while increasing Dunaliella salina cell density by the method for subsection filter.The method of the invention cultivation salt algae may advantageously facilitate the accumulation of nutritional ingredient in salt frond, have the advantages that salt algae yield is big, production cost is low, high value by-product is more, the high yield high value salt algae obtained while saving water resource and salt.
Description
Technical field
The present invention relates to salt algae cultural technique fields, and in particular to a kind of to utilize pickling waste water, solarization bittern water subsection filter
The method of salt algae.
Background technique
Dunaliella salina, that is, salt algae (Dunaliella salina) is very strong to the adaptability of osmotic stress, can be close
Fresh water in the environment of saturated brine until survive.Salt algae adapts to extraneous salinity by adjusting the glycerol concentration of cell interior
Variation, maintains the osmotic pressure of intraor extracellular, avoids dehydration dead.
It is existing research shows that can be accumulated in salt frond under extreme conditions in high salinity, strong illumination, nutrient missing etc.
A large amount of beta carotene or grease.The beta carotene of salt algae production is a kind of natural antioxidant, have delay senescence,
Prevent the characteristics such as disease, and saturated fatty acid and monounsaturated fatty acids content is higher substantially meets biodiesel in salt frond
Production standard.Since salt algae has resistance strong, the advantages that salt tolerance is high, and nutritional ingredient is high, in medicine, food, makeup
The industries such as product and feed processing are widely used.
Pickling waste water refers to marinated food generated waste water in the process of production and processing, although such discharge of wastewater water
It is smaller, but it contains a large amount of water soluble organic substance and soluble inorganic salt, high nitrogen phosphorus pickling waste water processing cost with high salt compared with
Greatly, if direct emission will appear soil hardening, salinization phenomenon, the water body and ecological environment to periphery are by serious destruction.It passes
The technique of evaporating brine of system is that seawater is first introduced evaporation tank, and crystallizing pond is poured into after solar evaporation moisture, continues solarization, stepped evaporation
Concentration, it is last until sodium chloride content can crystallize into salt close to saturation in brine.
There is toxigenic capacity height in current salt algae cultural method, salt algae yield and salt algae by-product yield can not mention simultaneously
High defect, it is therefore desirable to using the growth characteristics of salt algae as point of penetration, develop new technology to make full use of existing resource culture salt
Algae obtains the salt algae of high yield high value.
Summary of the invention
For overcome the deficiencies in the prior art, the present invention provides one kind and is not adding other nutrition such as sodium chloride and nitrogen phosphorus
In the case where element, using the method for pickling waste water and solarization bittern water subsection filter salt algae, is improving salt algae yield, promoting salt algae
While internal byproducts build-up, the purpose of energy-saving and emission-reduction is realized.
In order to solve the above technical problems, the technical scheme adopted by the invention is that:
A method of using pickling waste water, shining bittern water subsection filter salt algae, comprising the following steps:
(1) it configures adaptability culture solution: filtering, be uniformly mixed with less salt brine, high temperature after pickling waste water is adjusted pH to alkalescent
Salt algae adaptability culture solution is configured to after sterilizing；
(2) salt algae enrichment culture: after salt algae is seeded to the adaptability culture solution configured in step (1), culture salt algae is raw to logarithm
Algal gel is collected by centrifugation after long-term；
(3) it salt algae Fiber differentiation and collects: the algal gel collected in step (2) is seeded in high bittern water Fiber differentiation to stabilization
It is collected by centrifugation after phase.
Further, adjusting pH as described in step (1) pH value into alkalescent is 7-9.
Further, less salt brine as described in step (1) is the primary brine for being just emitted into evaporation tank, and salinity is
35-45 g/L。
Further, the salinity of adaptability culture solution as described in step (1) is 40-100 g/L.
Further, pickling waste water and less salt brine mixed volume ratio are 1:0.1-1 in step (1).
Further, the inoculum concentration of inoculation described in step (2) is 10-20% (v/v).
Further, step (2) the enrichment culture condition is full exposure, and intensity of illumination is 3000-5000 Lux.
Further, step (2), centrifugation described in (3) are 3000-6000 r/min centrifugation 10-30 min.
Further, high bittern water described in step (3) is the old brine being emitted into crystallizing pond after repeatedly evaporating,
Its salinity is 150-250 g/L.
Further, Fiber differentiation described in step (3) be initial pH 5-7, intensity of illumination 5000-10000 Lux,
12 hL:12 hD of periodicity of illumination.
Compared with prior art, the beneficial effects of the present invention are:
Method provided by the present invention according to dunaiella salina growth characteristic, make full use of pickling waste water, shine the sodium chloride in bittern water with
And the nutrients Fiber differentiation salt algae such as nitrogen phosphorus；High yield salt algae is first obtained using enrichment culture mode, is then shone in high salinity
Fiber differentiation promotes the accumulation of by-product in salt frond in bittern water, and the method by subsection filter is increasing Dunaliella salina cell density
While also improve the yield of the multi-functional by-product such as total rouge, beta carotene in salt frond.The present invention by pickling waste water again
Secondary utilization was not only preserved the ecological environment, but also high yield high value can be obtained without adding other nutriments in salt algae breeding process
Salt algae, so that solarization bittern water and pickling waste water are fully used, with salt algae yield is big, production cost is low, high value by-product
Advantage more than object, the high yield high value salt algae obtained while saving water resource and salt.
Detailed description of the invention
Fig. 1 is the Technology Roadmap using pickling waste water, solarization bittern water subsection filter salt algae.
Specific embodiment
With reference to the accompanying drawing and specific embodiment the present invention is further described, but protection scope of the present invention and unlimited
In this.Unless otherwise specified, reagent involved in the embodiment of the present invention is commercial product, can be purchased by commercial channel
Buy acquisition.
Embodiment 1:
(1) it prepares adaptability culture solution: the pH value of the pickling waste water of 55 g/L of salinity being adjusted to 7.0, by supernatant mistake after standing
It is uniformly mixed with the less salt brine that salinity is 43 g/L in 1:0.1 (v/v) ratio after filter, adaptability culture is configured to after high-temperature sterilization
Liquid；The salinity for measuring adaptability culture solution is 53 g/L, and total phosphorus concentration is 39.23 mg/L, and total nitrogen concentration is 425.13 mg/L,
Ammonia nitrogen concentration is 91.64 mg/L；
(2) salt algae: being seeded to the adaptability culture solution configured in step (1) by salt algae enrichment culture, and inoculum concentration is 10% (v/v), is fitted
The initial concentration of Dunaliella salina cell is 1.8 × 10 in raw culture solution5 Cell/mL, under the conditions of 5000 Lux of intensity of illumination, full exposure
Culture 12 days, dunaiella salina growth to logarithmic phase, 6000 r/min are centrifuged 15 min and collect algal gel；
(3) it salt algae Fiber differentiation and collects: the algal gel collected in step (2) is seeded to salinity is 150 g/L, initial pH is 5
High bittern water in, Dunaliella salina cell is dense after cultivating 4 days under the conditions of 10000 Lux of intensity of illumination, 12 hL:12 hD periodicity of illumination
Degree reaches 1.42 × 107 Cell/mL, 4000 r/min are centrifuged 15 min and collect salt algae.After measured, the total rouge yield of salt algae is
1.173 g/L, beta carotene yield are 12.73 mg/L.
Embodiment 2:
(1) it prepares adaptability culture solution: the pH value of the pickling waste water of 113 g/L of salinity being adjusted to 8.0, uses supernatant after standing
It is uniformly mixed with the less salt brine that salinity is 36 g/L in 1:0.5 (v/v) ratio after qualitative filter paper filtering, is configured after high-temperature sterilization
At adaptability culture solution；The salinity for measuring adaptability culture solution is 87 g/L, and total phosphorus concentration is 44.79 mg/L, and total nitrogen concentration is
358.83 mg/L, ammonia nitrogen concentration are 88.98 mg/L；
(2) salt algae enrichment culture: salt algae is seeded in the adaptability culture solution configured in step (1), and inoculum concentration is 15% (v/v),
The initial concentration of Dunaliella salina cell is 2.9 × 10 in adaptability culture solution5 Cell/mL, in 5000 Lux of intensity of illumination, full exposure condition
Lower culture 12 days, dunaiella salina growth to logarithmic phase, 6000 r/min are centrifuged 30 min and collect algal gel；
(3) it salt algae Fiber differentiation and collects: the algal gel collected in step (2) is seeded to salinity is 200 g/L, initial pH is 6
High bittern water in, Dunaliella salina cell concentration after being cultivated 4 days under the conditions of 8000 Lux, 12hL:12hD periodicity of illumination of intensity of illumination
Reach 1. 85 × 107 Cell/mL, 6000 r/min are centrifuged 30 min and collect salt algae.After measured, the total rouge yield of salt algae is 1.694
G/L, beta carotene yield are 18.49 mg/L.
Embodiment 3:
(1) it prepares adaptability culture solution: the pH value of the pickling waste water of 158 g/L of salinity being adjusted to 9.0, by supernatant mistake after standing
It is uniformly mixed with the less salt brine that salinity is 39 g/L in 1:1 (v/v) ratio after filter, adaptability culture is configured to after high-temperature sterilization
Liquid；The salinity for measuring adaptability culture solution is 98 g/L, and total phosphorus concentration is 39.94 mg/L, and total nitrogen concentration is 323.17 mg/L, ammonia
Nitrogen concentration is 93.65 mg/L；
(2) salt algae: being seeded to the adaptability culture solution configured in step (1) by salt algae enrichment culture, and inoculum concentration is 20% (v/v), is fitted
The initial concentration of Dunaliella salina cell is 3.8 × 10 in raw culture solution5 Cell/mL, under the conditions of 3000 Lux of intensity of illumination, full exposure
Culture 12 days, dunaiella salina growth to logarithmic phase；3000 r/min are centrifuged 10 min and collect algal gel；
(3) it salt algae Fiber differentiation and collects: the algal gel collected in step (2) is seeded to salinity is 250 g/L, initial pH is 7
High bittern water in, Dunaliella salina cell is dense after cultivating 4 days under the conditions of 5000 Lux of intensity of illumination, 12 hL:12 hD periodicity of illumination
Degree reaches 9. 03 × 106 Cell/mL, 6000 r/min are centrifuged 15 min and collect salt algae.After measured, the total rouge yield of salt algae is
0.652 g/L, beta carotene yield are 7.59 mg/L.
The above is only the preferred embodiment of the present invention, it is noted that above-mentioned preferred embodiment is not construed as pair
Limitation of the invention, protection scope of the present invention should be defined by the scope defined by the claims..For the art
For those of ordinary skill, without departing from the spirit and scope of the present invention, several improvements and modifications can also be made, these change
It also should be regarded as protection scope of the present invention into retouching.
Claims (10)
1. a kind of utilize pickling waste water, the method for solarization bittern water subsection filter salt algae, which comprises the following steps:
(1) it configures adaptability culture solution: filtering, be uniformly mixed with less salt brine, high temperature after pickling waste water is adjusted pH to alkalescent
Salt algae adaptability culture solution is configured to after sterilizing；
(2) salt algae enrichment culture: after salt algae is seeded to the adaptability culture solution configured in step (1), culture salt algae is raw to logarithm
Algal gel is collected by centrifugation after long-term；
(3) it salt algae Fiber differentiation and collects: the algal gel collected in step (2) is seeded in high bittern water Fiber differentiation to stabilization
It is collected by centrifugation after phase.
2. according to claim 1 utilize pickling waste water, the method for solarization bittern water subsection filter salt algae, which is characterized in that
Adjusting pH as described in step (1) pH value into alkalescent is 7-9.
3. according to claim 1 utilize pickling waste water, the method for solarization bittern water subsection filter salt algae, which is characterized in that
Less salt brine as described in step (1) is the primary brine for being just emitted into evaporation tank, and salinity is 35-45 g/L.
4. according to claim 1 utilize pickling waste water, the method for solarization bittern water subsection filter salt algae, which is characterized in that
The salinity of adaptability culture solution as described in step (1) is 40-100 g/L.
5. according to claim 1 utilize pickling waste water, the method for solarization bittern water subsection filter salt algae, which is characterized in that
Pickling waste water and less salt brine mixed volume ratio are 1:0.1-1 in step (1).
6. according to claim 1 utilize pickling waste water, the method for solarization bittern water subsection filter salt algae, which is characterized in that
The inoculum concentration of inoculation described in step (2) is 10-20% (v/v).
7. according to claim 1 utilize pickling waste water, the method for solarization bittern water subsection filter salt algae, which is characterized in that
Step (2) the enrichment culture condition is full exposure, and intensity of illumination is 3000-5000 Lux.
8. according to claim 1 utilize pickling waste water, the method for solarization bittern water subsection filter salt algae, which is characterized in that
Centrifugation described in step (2), step (3) is 3000-6000 r/min centrifugation 10-30 min.
9. according to claim 1 utilize pickling waste water, the method for solarization bittern water subsection filter salt algae, which is characterized in that
High bittern water described in step (3) is the old brine being emitted into crystallizing pond after repeatedly evaporating, salinity 150-250
g/L。
10. according to claim 1 utilize pickling waste water, the method for solarization bittern water subsection filter salt algae, which is characterized in that
Fiber differentiation described in step (3) is initial pH 5-7, intensity of illumination 5000-10000 Lux, 12 hL:12 of periodicity of illumination
hD。
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