WO2018070852A1 - Method for producing reduced-sodium salt containing active ingredient of dunaliella from salt pond water, and reduced-sodium salt produced thereby - Google Patents

Abstract

The present invention relates to: a method for producing from salt pond water, a reduced-sodium salt containing an active ingredient of Dunaliella; and a reduced-sodium salt produced thereby. According to the present invention, a reduced-sodium salt containing an active ingredient of Dunaliella, such as β-Carotene, and having a low sodium content can be produced at low cost. In addition, it is possible to efficiently produce high quality products having various contents of minerals and β-Carotene by adjusting the production process.

Description

Method for preparing low salt containing the useful component of Dunaliellae from salt water and low salt produced accordingly

The present invention relates to a method for preparing low salt containing a useful component of Dunaliella from salt water and to a low salt prepared according to the present invention, and more particularly to transplanting Dunaliella in high salt salt water. And it relates to a method for producing a low salt containing a useful component of Dunaliella, such as Dunaliella and beta-carotene by culturing and drying, and a low salt prepared accordingly.

Salt is indispensable to humans and consists of many minerals such as calcium, magnesium and potassium, as well as NaCl, the main ingredient. Although salt is collected from rock salt, a method of making it from abundant seawater has been used in Korea and other marine countries. Since sea salt is made from seawater in which all elements of the earth are dissolved, it depends on the decontamination method and contains various minerals. It is very important for human life to eat good salt with various minerals contained in salt.

On the other hand, in addition to salt and minerals contained in our salt, our bodies need many nutrients such as proteins, fats, carbohydrates and vitamins. Among them, antioxidants are currently attracting much attention regarding human health. Our body suffers from various diseases such as aging, cytoplasm degradation, and immunity deterioration due to the destructive action of cells and tissues in which persistent free radicals and peroxides occur constantly. Beta-carotene (β-Carotene), lutein (Lutein), chlorophyll (Chlorophyll), vitamins (Vitamin) and the like as a functional material that can prevent or alleviate these problems have been known to be effective.

In response to various demands of modern people, green tea salt, garlic salt, pepper salt, chitosan salt, potassium additive salt, iodine added salt, bamboo salt, etc. are provided in the process of making salt from seawater or by adding specific ingredients after decontamination. have. Salt made with addition salt or re-decontamination salt is used to improve the added value of salt through high-quality and to help humanity. Salt that contains carotene (β-Carotene), lutein (Lutein) efficiently is difficult to find.

On the other hand, the rapid consumption of food, such as western foods such as eating out, fast food and pizza, and food consumption of instant food, has increased due to the improvement of income level, nuclear familyization and the increase of single households. In particular, Koreans also consume high-sodium foods such as soup, stew, kimchi, soy sauce, and salted seafood.The daily sodium intake ranges from 6,000mg to 8,000mg, more than twice the WHO recommended amount (3,450mg). It is known to consume high concentrations of sodium, and excessive intake of sodium is a major cause of chronic diseases such as high blood pressure, heart disease, brain and cardiovascular disease, kidney disease, and stomach cancer.

Recently, attention has been paid to the reduction of sodium intake to prevent chronic diseases and to improve health, and as a result, researches on the development of low salt or salt enhancers are underway. For example, natural salts, minerals, seaweed, kelp, wolfberry, onion, shiitake mushroom, radish, garlic, etc., salt substitute natural seasoning, low salt with potassium chloride, calcium chloride, magnesium chloride instead of sodium There are chemical seasonings such as salt or aspartate and sodium sodium glutamate (MSG).

Accordingly, the present inventors have continued to develop a method for producing low salt while containing a large amount of useful ingredients of Dunaliella such as beta-carotene from high-salt salt water (Dunaliella) The present invention was completed by discovering that it is possible to produce low sodium salt containing useful ingredients of Dunaliella such as beta-carotene by transplanting and culturing) into salt water.

Therefore, the technical problem to be solved in the present invention is to provide a method for producing a low salt containing a useful component of Dunaliella using high salt salt water.

In addition, another technical problem to be solved by the present invention is to provide a low chlorine containing Dunaliella and the useful components thereof prepared according to the above method.

In order to solve the above technical problem, the present invention provides a method for producing a low salt salt containing Dunaliella and its useful components from salt water using Dunaliella (Dunaliella).

In addition, the present invention provides a low chlorine containing Dunaliella and the useful components thereof prepared according to the above method, in order to solve the above other technical problem.

Preferably, the present invention provides a method for producing low salt salt containing Dunaliella and its useful components, comprising the following steps:

(S1) pretreatment by sterilizing or filtering the salt water;

(S2) When the salinity (Salinity) of the pretreated salt water in step (S1) is 10 to 30 degrees (%), after adjusting the temperature of the salt water to 25 to 35 ℃ and then the Dunaliella salt water Implanting in the culture; And

(S3) drying the culture obtained in step (S2) to obtain a low salt containing Dunaliella and its useful components.

Preferably, the useful ingredient of the Dunaliella is beta-carotene (β-Carotene), alpha-carotene (α-carotene), lutein (lutein), zeaxanthin (zeaxanthin), cryptoxanthin (cryptoxanthin), chlorophyll, Omega-3 fatty acid, and the like, preferably beta-carotene (β-Carotene).

Preferably, low chlorine containing Dunaliella and the useful components thereof prepared according to the present invention is low chlorine having a Na content of 30% or less.

The salt water used in the present invention may have a salinity of 10 to 30 degrees (%), preferably 15 to 25 degrees (%), more preferably 17 to 25 degrees (%).

According to the present invention, salts having low sodium content and containing valuable substances such as beta-carotene (β-Carotene) at low cost by implanting and culturing Dunaliellae in high-salt salt water It is possible to produce, and by controlling the production process it is possible to efficiently produce a variety of high-quality products, such as minerals and beta carotene (β-Carotene).

1 and 2 are photographs showing the Dunaliella shape lyophilized by incubation at high salinity.

The present invention provides a method for producing low salt containing Dunaliella and its useful components from brine using Dunaliella.

In addition, the present invention provides a low chlorine containing Dunaliella and the useful components thereof prepared according to the above method.

Preferably, the present invention provides a method for producing low salt salt containing Dunaliella and its useful components, comprising the following steps:

(S1) pretreatment by sterilizing or filtering the salt water;

(S2) When the salinity (Salinity) of the pretreated salt water in step (S1) is 10 to 30 degrees (%), after adjusting the temperature of the salt water to 25 to 35 ℃ and then the Dunaliella salt water Implanting in the culture; And

(S3) drying the culture obtained in step (S2) to obtain a low salt containing Dunaliella and its useful components.

Preferably, the useful ingredient of the Dunaliella is beta-carotene (β-Carotene), alpha-carotene (α-carotene), lutein (lutein), zeaxanthin (zeaxanthin), cryptoxanthin (cryptoxanthin), chlorophyll, Omega-3 fatty acid, and the like, preferably beta-carotene (β-Carotene).

Preferably, low chlorine containing Dunaliella and the useful components thereof prepared according to the present invention is low chlorine having a Na content of 30% or less.

The salt water used in the present invention may have a salinity of 10 to 30 degrees (%), preferably 15 to 25 degrees (%), more preferably 17 to 25 degrees (%).

In the present invention, by culturing by directly transplanting Dunaliella (Dunaliella), which is viable at high salinity, directly to saline water, a low salt salt containing Dunaliella and useful components having a Na content of 30% or less can be obtained.

Dunaliella, a microalgae used in the present invention, is a microalgae that survives in a high salt environment. Dunaliella can now actively survive in African salt lakes, Israel's Dead Sea, and natural salt farms where the enrichment process is somewhat advanced. Therefore, high salt and high alkali environments are the optimum conditions for Dunaliella growth. Useful ingredients of Dunaliella include various nutrients in microalgae, for example, beta-carotene, alpha-carotene, lutein, zeak, as shown in Table 1 below. It may be zeaxanthin, cryptoxanthin, chlorophyll, omega-3 fatty acid, and the like, and preferably beta-carotene, but is not limited thereto.

division	main ingredient	Functional	Habitat environment (salin%)
Dunaliella (Green Algae)	Β-carotene, alpha-carotene, α-carotene, lutein, zeaxanthin, cryptoxanthin, chlorophyll, omega-3 fatty acid, etc.	Eye protection, anti-aging, immunity enhancement, UV protection of skin, antioxidant effect	3.3-31.0

Referring to step by step of the manufacturing method of low chlorine containing Dunaliella and its useful components of the present invention.

In the method for preparing low salt containing the useful ingredient of Dunaliella of the present invention, step (S1) is a step of sterilizing or filtering preliminary salt water, and sterilization may be performed by UV treatment, ozone treatment, or heat treatment. The present invention is not limited thereto, and the filtration may be performed using sand filtration, rapid filtration membrane, micro filter (MF), nano filter (NF), or ultra filter (UF), but is not limited thereto.

The sterilization or filtration process is to remove unnecessary microalgae that cannot survive harmful or high salinity in salt water, and to exclude the possibility of unnecessary consumption of nutrients contained in the salt water, such as sterilization or filtration. By performing the pretreatment process, only the denaliella to be cultured by removing microalgae other than the vinailianella that can survive at high salinity can be cultured in a clean state.

According to a specific embodiment of the present invention, by further performing the concentration step after the pretreatment step, it is possible to increase the content of nutrients required for the growth of Dunaliella to set up an environment that can promote the growth of Dunaliella. .

The concentration process uses a membrane separation method or a phase change method, and the membrane separation method may include reverse osmosis (RO), nanofiltration membrane (NF), electrodialysis (ED), and the like, and the phase change method may include a multi-stage flash type evaporation method ( MSF), multi-effect evaporation (MED), vapor compression evaporation (VCD), mechanical vapor recompression evaporation (MVR), vacuum cascade evaporation (VMEC), gas hydrate (GHF), An indirect (heat pump) type refrigeration method is mentioned, for example. This concentration process can be carried out once to three times.

According to one specific embodiment of the present invention, the pre-treated brine water in step (S1) is passed through a reverse osmosis membrane to prepare the first concentrated water and permeated water, and the first concentrated water is subjected to the secondary through the vacuum multi-evaporation concentration system. By concentration, high salinity and high nutrition secondary concentrated water can be prepared for Dunaliella to grow. The permeated water generated at this time can be commercialized separately for the purpose. The secondary concentration using the vacuum multi-stage evaporator is preferably concentrated for 8 to 12 hours at 500 ~ 700 mmHg vacuum and 50 ~ 70 ℃ temperature.

In the method for preparing low salt containing the useful ingredient of Dunaliella of the present invention, step (S2) is the temperature of the salt water when the salinity (Salinity) of the salt water pretreated in step (S1) is 10 to 30 degrees (%). It is adjusted to 25 to 35 ℃ and then dunaliella (Dunaliella) is implanted in saline water to incubate.

In this case, the salinity of the salt water may be 10 to 30 degrees (%), preferably 15 to 25 degrees (%), more preferably 17 to 25 degrees (%), the Dunaliella used in the present invention It is a bird that can survive in high salinity.

Nutritional medium may be additionally added to promote the culture of Dunaliella, and the nutritional medium that may be added at this time may be f / 2 medium, SOT medium, J / l medium, PES medium, Zarrouk medium. , Conwy medium, Schreiber medium and the like, but is not limited thereto. The nutrient media are media commonly used in the art.

According to one specific embodiment of the present invention, the culture process of Dunaliella (1) salt salt by forming an algae culture apparatus that can control the light (natural light or artificial light), air (CO ₂ ), temperature or salt concentration Adding water to the incubator and inoculating (grafting) Dunaliella; (2) absorbing nutrients in the process of culturing Dunaliella, and applying stress by changing the salt and temperature to increase the content of useful substances such as beta-carotene in Dunaliella cells.

According to one specific embodiment of the invention, the culture of Dunaliella (Dunaliella) in the salt water to an initial concentration of 20 x 10 ⁴ to 60 x 10 ⁴ cells / mL, preferably 50 x 10 ⁴ cells / mL After inoculation, it is preferable to incubate at 20 to 30 ° C. for 10 to 20 days in continuous light (natural light or artificial light) for 20 to 26 hours at 80 μmol Photon / m 2 s.

The implantation of Dunaliella can be cultured by implanting the temperature-controlled salt water into the algae culture and transplantation apparatus while diluting Dunaliella while diluting it.

Specifically, the temperature is adjusted to 1 ton of brine water cultured in the algae cultivation and implantation device is added to 1 ton. Therefore, two tons of salt water is present in the algae culturing and transplanting apparatus, and algae already cultured in the salt water are diluted. Since the diluted salt water is sent only one ton to the algae culture apparatus, which is the next process, the algae cultivation and transplantation device is left with the original one ton of salt water diluted with algae, and the other ton is transferred to the algae culture apparatus. do. In this process, new salt water was also added to the algae in the brine of the algae culture and transplantation apparatus, and then cultured on the basis of the nutrients contained in the algae, and the transferred cultured salt water algae were simultaneously cultured. After the incubation is completed, the culture and transplantation apparatus is supplied with freshly prepared secondary salt water, and the cultured Dunaliella is repeatedly transplanted by dilution.

Salt water containing Dunaliellae cultured in the culture apparatus is introduced into the algae stress applying process. Algal culture device refers to a device that can control the light, air (CO ₂ ) and temperature, etc. to incubate Dunaliella. When the cultivation of the salt water in the algae culture device is completed, it can be put into the algae stress supplying device. The content can be increased. After each incubation is completed, newly prepared salt water is added to the salt water of the algae culture and transplantation apparatus, and the salt water in the algae culture apparatus can be dried immediately or transferred to the algae stress applying apparatus and dried. Will continue to repeat.

The use of the temperature control medium in the thermostat in the whole process takes advantage of the low temperature of salt water. In addition, the temperature control medium used in the stressing device utilizes the high temperature energy of the salt water separated from the vacuum multi-stage evaporator.

In the method for preparing low salt containing the useful component of Dunaliella of the present invention, step (S3) is a step of obtaining low salt containing the useful component of Dunaliella by drying the salt water obtained in step (S2). In this case, the drying may be performed by spray drying, freeze drying, reduced pressure evaporation drying, flat bed method or sun salt method, but is not limited thereto. Preferably, the drying may be performed by spray drying.

In addition, the quality of the salt containing Dunaliella and its useful ingredient prepared by the method of the present invention is determined by the content of nutrients such as beta-carotene (β-Carotene) and the mineral content and balance of potassium, calcium, magnesium, etc. Depends on whether

According to the present invention, by implanting and culturing Dunaliera in salt water rich in minerals and nutrients to produce salts, it is possible to produce salts containing low sodium while containing useful substances such as beta-carotene (β-Carotene) at low cost. In addition, by controlling the production process it is possible to efficiently produce a variety of high-quality products, such as minerals and beta carotene (β-Carotene).

According to one specific embodiment of the present invention, the low chlorine prepared according to the present invention may increase the content of beta carotene in the low chlorine over time storage. For example, based on the implantation time of Dunaliella, beta-carotene content in low-salt salt was measured 6 to 14 weeks after transplantation, and it was confirmed that the content of beta-carotene increased with time.

Salt containing the useful component of Dunaliella prepared according to the method of the present invention may be used as it is, or may be prepared by using a functional low chlorine by adding a useful component.

In addition, by using the low salt salt containing the Dunaliella and the useful components of the present invention it can be produced a functional entertaining food containing a large amount of nutrients and maintains a salty taste but low sodium content.

Hereinafter, examples and the like will be described in detail to help understand the present invention. However, embodiments according to the present invention can be modified in many different forms, the scope of the invention should not be construed as limited to the following examples. Embodiments of the present invention are provided to more fully describe the present invention to those skilled in the art.

One. Salt water  Pretreatment and clean Concentrated water  Manufacture process

In the process of producing low salt salt containing useful substances such as beta carotene, it is important to prepare a high salt, high nutrition concentrated water that can grow Dunaliella at low cost. In addition, this process is a process of disinfection and sterilization by high temperature to remove microalgae other than Dunaliella in advance, so that the concentrated water sent to the culture and transplantation apparatus needs to be kept clean.

Some of the algae are beneficial but harmful, and there is a possibility of unnecessary consumption of the nutrients contained in the concentrated water. Therefore, some parts of the algae are removed in the pretreatment process and sterilized and sterilized by the high temperature of the concentration process. Removed.

The removal method was sterilization by high temperature in the pretreatment process using micro filter and UF filter and the concentration process using vacuum multi-stage evaporation method. Vacuum multistage evaporation was performed for more than 10 hours at a temperature of 60 ℃ or more proceeded to the concentration process. The evaporation concentration time can vary depending on the amount to be concentrated. After the pretreatment and incubation with concentrated water produced by sterilization at high temperature in vacuum multi-stage evaporation, no other algae growth was found.

As a result of this pretreatment and concentration process, plankton and algae are killed to increase cleanliness and nutrients are concentrated seawater. The salt water thus produced is a great environment for growing Dunaliella. As a result, the purified salt water can make mineral salts containing useful substances such as beta-carotene made from a single microalgae according to the salt manufacturer's choice without mixing other microalgae in Dunaliella culture.

2. Vacuum multi-steam evaporator  by Salt water  Produce

When the sterilized or filtered salt water is first passed through the reverse osmosis membrane (RO) in the pretreatment process, the first concentrated water and the permeated water (demineralized water) come out, and the permeated water can be commercialized according to its purpose separately. Primary concentrated water is used for the preparation, which has a specific gravity of 1.04 and a pH of about 8.5. The primary concentrated water is prepared by using a vacuum multi-stage evaporation concentrator (VMEC).

In order to save energy and reduce energy by low temperature concentration, the vacuum multi-evaporation concentrator (VMEC) was used.

Water usually boils at 100 ℃, but salt water contains various minerals, so its boiling point is higher than 100 ℃. The boiling point becomes higher because the density increases as the concentration progresses. Therefore, the amount of energy required for evaporation is further increased and various side effects may occur such as the possibility of the denaturation of minerals and nutrients contained in the salt water is increased.

In order to prevent this and increase energy efficiency, the vacuum cascade evaporator (VMEC) is used to vacuum the inside of the evaporator, thereby reducing the boiling point, thereby preventing these problems. The vacuum multi-stage evaporation concentrator (VMEC) is energy efficient and easy to control because the energy input amount can be determined according to the characteristics of the seawater at each stage. In addition, the concentration in the vacuum has the advantage of reducing mineral destruction and scale formation due to high temperature evaporation.

In other words, if the vacuum inside the vacuum multi-evaporation condenser evaporator is about 600 mmHg, the boiling point is lowered below 60 ° C. By lowering the boiling point, the energy input could be reduced by the difference, and the physicochemical change and scale of the mineral due to the high temperature could be reduced due to evaporation due to the low temperature. In addition, as a result of concentrating and maintaining the seawater for 10 hours or more using a vacuum multi-evaporation concentrator (VMEC), the growth of other microalgae was not found.

 The concentration method of the primary concentrated water using the vacuum multi-steam evaporator (VMEC) is described in more detail as follows. When the first concentrated water is concentrated in a first stage evaporator of a vacuum multistage evaporator (VMEC), calcium carbonate begins to crystallize as the concentration proceeds. Calcium carbonate does not melt well once it is crystallized, so it is not easy to dissolve and use again. In addition, there is a problem that does not melt well when added to food when included in salt. Therefore, it is good to separate and extract calcium carbonate and use it for other purposes than salt. In addition, since it is the cause of the scale attached to the inside of the equipment, it is helpful to separate the extraction to enhance the equipment operating efficiency.

When the salt water is sterilized or filtered and concentrated in this manner, plankton and algae are killed to make the salt water highly clean. The salt water thus produced is a great environment for culturing Dunaliella. As a result, the purified concentrated brine can produce salts containing useful substances such as beta-carotene (β-Carotene) made by a single microalgae according to the salt manufacturer's choice without mixing other microalgae in the culture of Dunaliella. Can be.

Alternatively, according to one specific embodiment of the present invention, in the present invention, it is possible to use water collected in concentrated water or salt evaporation basin in a region producing sun salt in addition to salt water.

3. Salt water  Temperature control and Dunaliella  Culture process

Temperature is very important in the growing condition of Dunaliella. Dunaliella grows in a high temperature environment, so it needs to create a high temperature environment. Because these conditions are difficult to formulate in everyday environments, despite the high utility of these microalgae, cultivation has been carried out only under certain circumstances in certain regions. The concentration process to make salt requires a lot of energy. And in the meantime, a lot of energy has actually been abandoned. In the present invention, economical production of salts containing useful substances such as beta carotene is made possible by utilizing these discarded low or high temperature energy.

Salt water, which has become a high salinity and high alkali through pretreatment and concentration, has a high temperature close to 60 ℃, and microalgae cannot grow in such a high temperature environment. Therefore, the concentrated salt water should be made at a temperature of about 30 ° C. to cultivate Dunaliella.

In addition, culturing Dunaliella requires considerable time. It may take at least one day to cultivate the required amount of Dunaliella, and several days to cultivate dense Denaliella. In this case, it is necessary to maintain a constant temperature around 30 ℃ required for the growth of microalgae. Therefore, efforts to continuously maintain the temperature are required and enormous energy is required. The energy required is efficient using waste heat from the evaporator. As the evaporator condenses the brine water, a huge amount of water vapor is emitted, which can be recovered in the form of hot condensate. This energy can be sent to the algae cultivation device on the one hand to control the temperature used for stressing while keeping the temperature of the culture device constant while lowering the high temperature of the condensate.

This makes it possible to maintain a constant temperature of the culture apparatus without additional energy input. Therefore, it is possible to cultivate microalgae at an economical cost as possible systemically possible conditions in the tropics, and as a result it is possible to manufacture salt containing useful substances such as beta carotene (β-Carotene) at low cost.

The implantation of Dunaliella is performed even when the salinity of saline water is 10-30 degrees (%), preferably 15-25 degrees (%), more preferably 17-25 degrees (%). Dunaliella) can be cultured by implanting in brine. At this time, the transplantation may be inoculated to an initial concentration of Dunaliella 20 x 10 ⁴ to 60 x 10 ⁴ cells / mL, and then cultured at 20 to 30 ℃ for 10 to 20 days under natural light or fluorescent lighting.

< Example  1> Dunaliella  Used Low salt  Produce

(1) Salt water was sterilized using UV, ozone treatment or heat, or filtered through a microfilter (MF) or ultrafilter (UF).

(2) When the salinity (Salinity) of the pretreated salt water is 17 degrees (%), the temperature of the salt water is adjusted to 25 to 35 ℃ and then Dunaliella (Dunaliella) in the salt water initial concentration 20 x 10 ⁴ After inoculating to cells / mL, low chlorine was prepared by incubating at 25 ° C. for 15 days in natural light at 80 μmol Photon / m ² s for 15 hours under continuous light.

1 and 2 are photographs showing the Dunaliella shape lyophilized by incubation at high salinity.

< Example  2> Low salt  Measurement of Beta Carotene Content by Storage Time

Beta carotene (β-Carotene) content was measured while storing the low salt prepared in Example 1, and is shown in Table 2 below.

After transplant	Beta-carotene content in Dunaliella salt (Carotenoid level (ug / g FW)
6 Weeks	3
Week 7	5
8 Weeks	9
9 Weeks	18
Week 11	23
14 Weeks	59

As shown in Table 2, it was confirmed that the content of beta carotene in the Dunalella salt prepared according to the present invention increases with the storage time.

< Example  3> Low salt  Na content measurement

The Na content in the low salt prepared in Example 1 was measured and shown in Table 3 below.

	Na content (US EPA 3052A, 6010C (ICP): 2007)
Dunaliella Salt	18 to 24

As shown in Table 3, the salt prepared according to the present invention can be confirmed that the Na content is a low chlorine as the highest 24%.

< Example  4> Low salt  Sensory evaluation

The sensory test results of the salt of the present invention Dunaliella and its useful components are shown in Table 4. Thirty housewives in their 4s to 50s had low salt and microalgae containing the salt of the present invention by Dunalella and its useful ingredients by the 5-point rating method. Salty and overall palatability were tested. The results are shown in Table 4 below. Sensory evaluation results were expressed as a measurement value (1: bad, 2: a little bad, 3: normal, 4: a little good, 5: good).

Kinds	color	incense	flavor	Change of sign after explanation
Control group 1	3	3	3	2
Control 2	4	3	5	4
Dunaliella low salt	4	4	5	5

As shown in Table 4, the low salt salt prepared according to the present invention was confirmed that the difference in salty taste is similar to the existing salt, although the Na content is not significant.

According to the present invention, by directly culturing Dunaliella in high-salt salt water and incubating salt to produce salt, it contains a useful substance of Dunaliella such as beta-carotene (β-Carotene) and has a low sodium content. It is possible to produce low salt, and by controlling the production process, it is possible to efficiently produce high-quality products with various contents of minerals and beta-carotene (β-Carotene).

Claims (10)

1. Method for producing low salt containing Dunaliella and its useful components from salt water using Dunaliella (Dunaliella).
2. The method of claim 1,

   Method for producing a low salt salt containing Dunaliella and its useful components, characterized in that it comprises the following steps:

   (S1) pretreatment by sterilizing or filtering the salt water;

   (S2) When the salinity (Salinity) of the pre-treated salt water in step (S1) is 10 to 30 (%) degrees to adjust the temperature of the salt water to 25 to 35 ℃ and then Dunaliella (Dunaliella) to the salt water Transplanting and culturing; And

   (S3) drying the culture obtained in step (S2) to obtain a low salt containing Dunaliella and its useful components.
3. The method of claim 2,

   Sterilization in the step (S1) is characterized in that carried out by UV treatment, ozone treatment or heat treatment method.
4. The method of claim 2,

   Filtration in the step (S1) is characterized in that carried out using a sand filtration, rapid filtration membrane, micro filter (MF), nano filter (NF) or ultra filter (UF).
5. The method of claim 2,

   Further comprising the step of concentrating after the pretreatment of step (S1).
6. The method of claim 2,

   In the step (S2) Dunaliella (Dunaliella) inoculated in salt water to an initial concentration of 20 x 10 ⁴ to 60 x 10 ⁴ cells / mL and then incubated at 20 to 35 ℃ for 10 to 20 days under natural or artificial light Characterized in that the method.
7. The method of claim 2,

   The drying of the step (S3) is characterized in that it is carried out using spray drying, freeze drying, reduced pressure evaporation drying, flat bed method or sun salt method.
8. The method of claim 1,

   Useful ingredients of the Dunaliella are beta carotene (β-Carotene), alpha carotene (α-carotene), lutein (lutein), zeaxanthin, cryptoxanthin, cryptoxanthin, chlorophyll and omega 3 fatty acids (omega-3 fatty acid) at least one selected from the group consisting of.
9. A low chlorine containing Dunaliella and a useful component thereof prepared according to the method according to any one of claims 1 to 8.
10. The method of claim 9,

    Low chlorine, characterized in that the Na content in the low chlorine is less than 30%.

Images