CN101691547A - Method for restoring micro-scale polluted reservoir water by using situ bio-contact oxidation - Google Patents

Abstract

The invention discloses a method for restoring micro-scale polluted reservoir water by using situ bio-contact oxidation. The method comprises the following steps: adopting fiber and sponge combined ball-type packings which are independently developed as carriers to perform screening, placing high effective strain which is obtained through screening in culture solution 'HS enrichment medium' to perform enrichment culture, then inoculating the strain which is through the enrichment culture in 'HSK culture medium' to perform expending cultivation and prepare biological microbial inoculum, placing packings in the microbial inoculum to perform biofilm formation and obtain biological immobilized packings; connecting the prepared biological immobilized packings with a string to fix the packings around the laboratory patented device water-lifting aerator of the invention and adopting water-lifting aeration-situ bio-contact oxidation to perform bioremediation to the micro-scale polluted reservoir water. The method of the invention can be used to effectively reduce the concentrations of COD, N, P and other pollutants in reservoir water and especially effectively lower the total nitrogen content of the micro-scale polluted reservoir water so as to fundamentally resolve the pollution problem of reservoir water, inhibit the growth of algae and control water eutrophication.

Description

Adopt the method for situ bio-contact oxidation restoring micro-scale polluted reservoir water

Technical field

The invention belongs to water-treatment technology field, be specifically related to a kind of method that adopts the situ bio-contact oxidation restoring micro-scale polluted reservoir water, be specially adapted to take place the micro-polluted water storehouse and the lake water of eutrophication.

Background technology

The pollution of source water is the problem that generally faces in world today's scope, China's 7 big water systems and 110 emphasis sections, landlocked river, III, IV, V class water quality account for 68%, China's nearly 1/2 river length in existing river is polluted, 1/10 river length is subjected to severe contamination, National urban 90% waters is polluted, and the master stream, great river accounts for 13%, and tributary 55% is contaminated.Contaminated water source is serious day by day, and organic content such as nitrogen, phosphorus exceed standard and the water quality eutrophication that causes thus is the subject matter that present China water pollutes.Strengthening water conservation and improving water technology is to improve drinking water quality, guarantees two effective measure of safety water supply.

Micro-polluted source water is meant and is subjected to Organic pollutants that the index of some projects surpasses hygienic standard.Pollutant kind contained in this class water is more, character is complicated, but concentration ratio is lower.The environmental problem that lake, reservoir eutrophication and blue-green alga bloom outburst cause arouses extensive attention at home and abroad, adopting the bio-contact oxidation technology is to handle the important method of micro-polluted source water, but the bio-contact oxidation technology of present source water both domestic and external all belongs to the heterotopic biological recovery technique basically, certainly will build the processing structures building to increase in the water factory like this, often to be subjected to the restriction of factors such as place, improve cost of water treatment, and can not fundamentally solve the serious day by day problem of waterhead area pollution of waterhead.

Summary of the invention

Defective or deficiency at the prior art existence, the object of the invention is, a kind of method that adopts the situ bio-contact oxidation restoring micro-scale polluted reservoir water is provided, pollute serious day by day to solve the waterhead area source water, pollutents such as COD, N, P exceed standard, body eutrophication frequently takes place, the problem of serious harm drinking water safety and HUMAN HEALTH.This method can effectively reduce the concentration of pollutents such as COD, N, the P of source water, solves the source water pollution problems from the source.Reduce running cost simultaneously, suppress algal grown, the control body eutrophication.

In order to realize above-mentioned task, method of the present invention is to be achieved by the following technical programs:

A kind of microorganism that is used for restoring micro-scale polluted reservoir water, it is characterized in that, described microorganism is nitrobacteria SY1 (Pseudomonas sp.SY1), deposit number is: CCTCCNO:M 209180 and denitrifying bacterium HY1 (Pseudomonas sp.HY1), deposit number is: CCTCCNO:M 209181.

A kind of method that adopts the situ bio-contact oxidation restoring micro-scale polluted reservoir water is characterized in that, comprises the following steps:

1) select biologic packing material as microorganism carrier;

2) preparation of bacteria agent HS: will from the reservoir bed mud of Heihe, screen and obtain nitrobacteria SY1 (Pseudomonas sp.SY1, deposit number is: CCTCCNO:M 209180) and denitrifying bacterium HY1 (Pseudomonas sp.HY1, deposit number is: CCTCCNO:M 209181) be inoculated on the HS enrichment medium, after 2-3d is cultivated in the constant temperature vibration, bacterial classification is transplanted enlarged culturing in the HSK substratum, and 2d is cultivated in the constant temperature vibration;

The prescription of described HS enrichment medium is: NaAc 0.5g, NaNO ₃0.1g, NH ₄Cl 0.1g, MgSO ₄0.02g, CaCl ₂0.02g, K ₂HPO ₄0.05g distilled water is settled to 1L, pH transfers to 7-7.5;

The prescription of described HSK substratum is: NaAc 0.1g, NaNO ₃0.02g, NH ₄Cl 0.02g, K ₂HPO ₄0.01g, sterilization source water 0.3-0.4L, distilled water is settled to 1L, and pH transfers to 7-7.5;

3) microorganism colonization: place the microbial inoculum of usefulness " HSK substratum " enlarged culturing to carry out bio-film colonization the biologic packing material of selecting, bio-film colonization is controlled at 2-3mg/L by aerating apparatus with the concentration of DO, and every day, the nutrient solution of 30-40% was changed to pending source water, reduce nutrient concentrations gradually, make microorganism SY1 in the biologic packing material and HY1 adapt to the water quality condition of micro-polluted source water, continuous aeration biofilm 4-5d, treat that biological filler forms that biofilm finishes behind the yellow microbial film, adopt solid stream of water to wash 4～5 times continuously, wash out the bacterium of the absorption of filling surface, make the biological fixnig filler;

4) situ bio-contact oxidation restoring micro-scale polluted reservoir water: the biological fixnig filler for preparing is connected and fixed on around the aerator that pumps up water with rope, adopts and pump up water aeration-situ bio-contact oxidation to carrying out biological restoration in the micro-polluted source water.

The technology of situ bio-contact oxidation restoring micro-scale polluted reservoir water of the present invention has compared with prior art that to handle the water yield big, and the treatment time is short, stable effluent quality, and running cost is low, has avoided increasing building the processing structures.Can effectively reduce the concentration of the pollutent such as COD, N, P of source water, particularly can effectively remove the total nitrogen content in the micro-polluted source water.Solve the source water pollution problems from the source, suppress algal grown, the control body eutrophication.

Description of drawings

Fig. 1 is the COD of test experience of the present invention _MNThe variation diagram of concentration;

Fig. 2 is the NH of test experience of the present invention ₄ ⁺The variation diagram of-N concentration;

Fig. 3 is the NO of test experience of the present invention ₃ ^-The variation diagram of-N concentration;

Fig. 4 is the NO of test experience of the present invention ₂ ^-The variation diagram of-N concentration;

Fig. 5 is the variation diagram of the TN concentration of test experience of the present invention;

Fig. 6 is the turbidity variation diagram of test experience of the present invention;

Fig. 7 is the part microorganism photo on the test experience filler of the present invention, and the magnification of each figure is 40 * 10 times.Wherein figure (a) is a wheel animalcule, and figure (b) is a campanularian, and figure (c) is a nematode.

The present invention is described in further detail below in conjunction with drawings and Examples.

The high-efficiency strain that the present invention relates to screen obtains nitrobacteria SY1 and denitrifying bacterium HY1 for screening from the reservoir bed mud of Heihe, these two bacterial classifications are preserved in Chinese typical culture collection center on August 24th, 2009, be called for short CCTCC, and register on the books, this biological inoculum was preserved 30 years on August 24th, 2009.Wherein nitrobacteria SY1 (Pseudomonas sp.SY1) preserving number is: CCTCCNO:M 209180; Denitrifying bacterium HY1 (Pseudomonas sp.HY1) preserving number is: CCTCCNO:M 209181.

Embodiment

Situ bio-contact oxidation restoring micro-scale polluted reservoir water technology of the present invention, its mentality of designing is that (patent No. is: ZL200410073541.3) combine with the aerator that pumps up water with applicant's independent research with the bio-contact oxidation technology, not only satisfied the needed aerating system design requirements of situ bio-contact oxidation restoring micro-scale polluted reservoir water technology, and can be implemented in N, the P content that can effectively reduce when mixing upper and lower water layer in the water, the control body eutrophication, avoided increasing and built the processing structures, reduced the purpose of running cost.

What use in experimental study and domestic large-scale actual engineering at present mainly is heterotopic biological catalytic oxidation technology, substantially all can reach more than 70% for the ammonia nitrogen removal in the micro-polluted source water, organic matter removal can reach 20-30%, but handling, dystopy certainly will increase the cost that produces owing to construction water factory structures, and lower to the removal efficient of total nitrogen.

How can adopt comprehensive method to improve raw water quality in source water, to stop the generation of problems such as eutrophication and endogenous pollution release, thereby replace or reduce former water post-processed program, the emphasis of research be a water quality original position reparation improvement technology at present.The original position reparation need not to carry polluted-water, the characteristics that this technology has instant effect, reduced investment, has no side effect.Aeration and the bio-contact oxidation combination technique of pumping up water given full play to function separately, plays a series of effects such as oxygenation, mixing, oxygenolysis, the release of inhibition bed mud and algal grown, thereby reaches the effect that improves water quality, control body eutrophication.

Following above-mentioned principle of work the present invention takes following technical scheme to be achieved:

A kind of method that adopts the situ bio-contact oxidation restoring micro-scale polluted reservoir water comprises the following steps:

1) select biologic packing material as microorganism carrier.

The present invention selects the biologic packing material of this area routine, for example Yixing Yulong Environmental Protection Co., Ltd. (address: high Left foreign investment industry park, Yixing, Jiangsu) a kind of novel microorganism membrane carrier of Sheng Chaning, this biologic packing material specific surface area is big, easy to be filmed, difficult drop-off, good hydrophilic property.Proportion after the biofilm approaches 1, can obtain fluidization completely under aeration condition, and free unobstructed rotation increases bump and cutting to underwater bubble in water, improves the utilization ratio of oxygen.The concrete physical and chemical index of biologic packing material is as shown in table 1:

The physicals of table 1 biologic packing material

2) preparation of bacteria agent HS: will from the reservoir bed mud of Heihe, screen high-efficiency strain nitrobacteria SY1 (the Pseudomonas sp.SY1 that obtains, deposit number is: CCTCCNO:M 209180) and denitrifying bacterium HY1 (Pseudomonas sp.HY1, deposit number is: CCTCCNO:M 209181) be inoculated in the HS enrichment medium, 2-3d is cultivated in the constant temperature vibration under 25-30 ℃, 120rpm condition, see obvious growth is arranged, be the absorbance of bacteria suspension, generally when OD600＞0.5, stop to cultivate at the 600nm place.Be inoculated in enlarged culturing in the HSK substratum then, 2d is cultivated in the constant temperature vibration under 25-30 ℃, 120rpm condition.

Wherein nitrobacteria SY1 and denitrifying bacterium HY1 bacterial strain all belong to Rhodopseudomonas, and it has following feature separately:

(a) colony morphology characteristic and morphological features are referring to table 2.

Strain number	Colony characteristics	Colony diameter (MM) when cultivating 4D	The thalline shape
				????HY1	Oyster white, circular edge is neat	????3	Tyrothricin
????SY1	Middle yellow fraction, transparent on every side, circle, neat in edge	????2.5×1.	Tyrothricin

Table 2

(b) major physiological, biochemical character, referring to table 3:

	The starch hydrolysis	Produce ammonia and show positive	The sugar alcohol fermentation and acid	Aerogenesis not	Milk reindeer moss shows alkalescence	Rennin solidifies	The methyl red positive
								SY1	??√	????√	+	√	√	√	??√
HY1	??√	????√	+	√	√	√	??√

Table 3

(annotate: √ expresses support for, and+expression is grown or reacted positive)

(c) NH ₄ ⁺-N, NO ₃ ^--N clearance

The ammonia nitrogen starting point concentration is 1.26mg/L, is 0.0112mg/L through ammonia nitrogen ultimate density after the cultivation of 48h, is 99.11% to ammonia nitrogen removal frank.

The nitrate starting point concentration is 2.3mg/L, is 0.041mg/L through nitrate ultimate density after the cultivation of 48h, is 98.52% to the nitrate removal rate.Referring to table 4:

But bacterial strain	Starting point concentration (mg/L)	Ultimate density (mg/L)	Clearance (%)
				SY1	1.26	0.0112	99.11
HY1	2.3	0.041	98.52

Table 4

The prescription of described HS enrichment medium is: NaAc 0.5g, NaNO ₃0.1g, NH ₄Cl 0.1g, MgSO ₄0.02, CaCl ₂0.02g, K ₂HPO ₄0.05g distilled water is settled to 1L, pH transfers to 7-7.5.

The prescription of described HSK substratum is: NaAc 0.1g, NaNO ₃0.02g, NH ₄Cl 0.02g, K ₂HPO ₄0.01g, sterilization source water 0.3-0.4L, distilled water is settled to 1L, and pH transfers to 7-7.5.

3) microorganism colonization: place the microbial inoculum of usefulness " HSK substratum " enlarged culturing to carry out bio-film colonization the filler of preparation, biofilm is controlled at 2-3mg/L to quicken the biofilm process by aerating apparatus with the concentration of DO, every day, just HSK substratum total amount 30-40% nutrient solution was changed to pending source water, reduce nutrient concentrations gradually, make microorganism adapt to the water quality condition of micro-polluted source water, continuous aeration biofilm 4-5d, biofilm finished after biologic packing material formed yellow microbial film, adopt solid stream of water to wash 4～5 times continuously, wash out the bacterium of the absorption of filling surface, make the biological fixnig filler.

4) situ bio-contact oxidation restoring micro-scale polluted reservoir water: the biological fixnig filler for preparing is connected with rope, be fixed in this laboratory Patent equipment and pump up water around the aerator, adopt and pump up water aeration-situ bio-contact oxidation carrying out biological restoration in the micro-polluted source water.

Experimental verification:

Abide by above-mentioned steps of the present invention and technical essential, the present invention as experimental water, carries out denitrogenation processing to it with the Xi'an source water, and the situation of specifically testing is as follows:

1, raw water quality

Its water-quality guideline of water source, Xi'an meets the micro-polluted water scope, and the concrete parameter of the every index of water quality sees Table 5:

Table 5 raw water quality situation

2, experimental installation

Testing apparatus is 2 cylindrical synthetic glass containers, diameter diameter 300mm, and high 800mm, useful volume is 50L.Wherein 1 is blank, and any biologic packing material promptly is not housed; Another is equipped with the high-performance bio filler.Container top has thief hole, venting hole and solarization air cap, and pressurized air enters from top through the aeration scatterer, regulates aeration rate with air flowmeter, and the DO value of system is controlled between 2.5～3.0mg/L, and temperature is 27.5 ℃,, pH value is 7.58.The blank device need not aeration.

3, biologic packing material

The Φ 25 Yue Loong floating stuffings of selecting for use Yixing Yulong Environmental Protection Co., Ltd. to produce, filling ratio is 2%.

4, experimental implementation, and result

Follow technological step of the present invention, adopt national standard method to measure CODMn, NH4+-N, NO3--N, NO2--N and TN.Adopt PHS-3C type precision acidity meter to measure pH value, adopt HQ-30d type dissolved oxygen meter to measure DO, adopt the WGZ-1S turbidimeter to measure turbidity.Through 43 days operation, its test-results showed as follows:

4.1COD the variation of removal effect

Referring to Fig. 1, after adapting to after a while, the clearance of CODMn between 8.7%-25.3%, the minimum 4.75mg/L that reduces to of concentration.This mainly be since situ bio-contact oxidation by microorganism self life Metabolic activity---oxidation, reduction, process such as synthetic, the biofloculation of microorganism, absorption, oxidation and comprehensive action such as the nitrated organic pollution materials [4] in dewatering of making a return journey.Removing organic mushroom mainly is heterotrophic bacteria, and they make biodegradable organism generation oxidizing reaction, thereby make the carbon in the organism be oxidized to carbonic acid gas, and organic ammonia nitrogen is oxidized to the nitrogen of ammonia attitude.Most of algae in the former in addition water can utilize sunlight to carry out photosynthesis by day, consumes part organism in the water body, plays the effect of purifying water body.Protozoon that adheres on the filler and miniature metazoan are food with algae detritus, mushroom and organic debris mainly, and the removal of organic substance is also had certain contribution.Common membrane structure and the function that constitutes on the filler such as mushroom, algae, microfauna.

4.2NH ₄ ⁺The variation of-N removal effect

Referring to Fig. 2, under an amount of aeration and poor nutritional condition, ammonia nitrogen still has more stable removal effect.The ammonia nitrogen concentration that packed column reactor was housed on the 1st day is higher than the blank value, and this is because the loose bacterium in floating stuffing top layer enters due to the water body.It may be because the adsorption of filling surface that the 3rd day ammonia nitrogen concentration value reduces, in the time of the 5th day, ammonia nitrogen concentration rises, mainly be in the former water a large amount of microorganisms to organic quick decomposition, causing a large amount of organonitrogens to be converted into ammonia nitrogen rapidly enters in the former water and causes, and ammonia nitrogen mainly relies on the effect of nitrobacteria to the conversion of nitric nitrogen, because the nitrobacteria in the former water is strict autotrophic type microorganism, its adaptation and growth metabolism speed to environment is all slow, this fashion of nitrification is not brought into play, and it is higher to show as initial period ammonia nitrogen value.By the 15th day, the ammonia nitrogen concentration value of each reactor dropped to minimum, and the place to go rate of ammonia nitrogen is reached about 75%.The concentration of ammonia nitrogen remains on about 0.1mg/L substantially after the 17th day, illustrates that the nutritive substance in the water body exhausts.To about the 37th day, the ammonia nitrogen concentration value increases suddenly, analyze its reason and may be since filler top aged biofilm detachment in the former water due to.

4.3NO ₃ ^-The variation of-N removal effect

As shown in Figure 3, because the denitrification denitrogenation effect of high-effective microorganism makes the Kaldnes floating stuffing have good Degradation to the nitrate nitrogen in the former water.The maximum material removal rate of nitre nitrogen occurs in the 31st day, reaches about 80%.Tested the 1st day, the nitrate nitrogen concentration of packed column reactor is higher than the blank value, and this is because the loose bacterium in floating stuffing top layer enters due to the water body.It may be because the adsorption of filling surface that the 3rd day nitrate nitrogen concentration reduces, increase along with the time, because the effect of nitrobacteria, make the ammonia nitrogen in the former water be converted into nitrate nitrogen, therefore occurred the increase of nitrate nitrogen value in about the 15th day, and this moment, ammonia-N removal rate also reached maximum value.In after this 12 days, it is stable that the nitre nitrogen concentration keeps, biological degradation is not obvious, this is because microbial growth all has certain rules and cycle, and under the situation that poor nutritional status or nutrition are restricted, microorganism has a long-term process that adapts to, in this change procedure, noticeable change all will take place in biological form and physiology, and reach stable hunger---survival condition, and cell is big or small basicly stable at this moment.Through the cell of hungry existence, because energy deficiency, the efficient of metabolic mechanism is improved, and therefore the utilising efficiency to matrix improves, and can reach better denitrification effect.The high-performance bio microbial inoculum that this test is adopted is poor nutrition denitrifying bacterium, and the generation cycle is long, therefore to the better adaptability of environment, in biological denitrification process nitrate is converted into harmless nitrogen, a spot of nitrite nitrogen and oxynitride and is removed.After 31 days, the nitre nitrogen concentration slightly raises, and reason may cause to former water owing to filler top aged biofilm detachment; After 39 days, the nitre nitrogen concentration tends towards stability substantially, biomembranous come off and growth reaches dynamic balance state.

4.4NO ₂ ^-The variation of-N removal effect

In preceding 27 days of system's operation, the nitrite nitrogen change in concentration is little referring to Fig. 4, and this is owing to ammonia nitrogen concentration in the former water is very low, NH under the effect of nitrococcus ₄ ⁺-N is oxidized to NO ₂ ^--N, and then utilize oxygen in the water with NO by nitrifier ₂ ^--N is oxidized to NO rapidly ₃ ^--N.Nitrite nitrogen concentration increased suddenly in the 29th day, and this moment, the nitre nitrogen removal efficiency was higher as seen from Figure 3, and denitrification is remarkable, so this part nitrite nitrogen is produced in denitrification process by nitre nitrogen.The 31st day nitrite nitrogen concentration value approached zero, after this raises rapidly, reached maximum value by 41 days, this be since this moment former water ammonia nitrogen increase suddenly, and nitrobacteria growth speed is slower than nitrococcus, this moment can't be with nitrococcus by NH ₄ ⁺That part of NO that-N is oxidized to ₂ ^--N is oxidized to NO in time ₃ ^-Therefore-N causes NO ₂ ^-The accumulation of-N.

4.5TN the variation of removal effect

Fig. 5 under the effect of poor nutrition denitrifying bacteria, strengthens the situ bio-contact oxidation method total nitrogen is had significant Degradation as can be seen.Oligotrophic microorganisms in the high-performance bio microbial inoculum has bigger advantage in nutraceutical competition, lower concentration matrix is had higher relatively avidity, high adsorption capacity, and speed is fast, and absorptive capacity is big.The biodegradable organism of lower concentration, ammonia nitrogen, nitre nitrogen, nitrite nitrogen etc. are at first very fast adsorbed by the microbial film on the filler in the water, aerobic and facultative oligotrophic bacteria on the rapid then tunicle absorbs, by the nitrogenous source pollutent in comprehensive actions such as biological degradation, the nitrification and denitrification removal water.The concentration curve of comparison diagram 5 and Fig. 3 as can be seen, total nitrogen is more close with nitre nitrogen concentration variation tendency, this is because organonitrogen, ammonia nitrogen and nitrite nitrogen concentration in the former water are very low, and the nitre nitrogen concentration is higher relatively, so the degradation effect of nitre nitrogen has a significant impact nitrogen removal rate.The 1st day, it was because the loose bacterium in floating stuffing top layer enters due to the water body that packed column reactor total nitrogen value is higher than the blank value.Because high-efficiency strain is to the adaptation of poor nutrient environment, in preceding 27 days of reactor operation, total nitrogen concentration changes little, and the clearance of total nitrogen is 77.6% during by the 31st day, and total nitrogen concentration is about 0.5mg/L, and nitrogen removal rate keeps stable after 41 days.

4.6 the variation of turbidity removal effect

As seen from Figure 6, there is removal effect preferably in the situ bio-contact oxidation system to raw water turbidity, and at system's run duration, raw water turbidity is reduced to 1.80NTU by 18.50NTU, and clearance reaches 90.3%.

The removal of raw water turbidity mainly is biomembranous adsorption on the filler, microorganism throwing out, biological degradation and direct precipitating action.The organism of part formation turbidity is adsorbed by the microbial film on the filler in the former water, by the life Metabolic activity of microorganism, organic matter degradation is synthesized the organic cellular material of self, and raw water turbidity is reduced.And the colloidalmaterial in the former water, suspended particle etc. are because biomembranous adsorption, biofilm surface at filler forms the biofloculation body, produce intergranular phase mutual coagulation, form bigger particle, make that tiny suspended particle number reduces greatly in the water, bigger flocculated particle is desorption under the air water souring, sinks to the bottom.Because suspended substance is adsorbed at biofilm surface, along with time increase film surface can form tight zone gradually, hinder the mass transfer of microbial film and water pollutant and DO, reduce the total effective area of filler, thereby influenced the denitrification effect of system.Therefore more when former water suspension, turbidity is necessary filler to be washed when big in good time.

5, microbial film feature and function analysis

The Yue Loong suspending carrier of newly purchasing is a whitish translucent material; Preceding 2 weeks of reactor operation are because high-efficiency strain has the adaptive process of a long period to poor nutrient environment, so the microbial film variation in thickness is not obvious, and filling surface is faint yellow, finds to have a large amount of bacteriums during microscopy, and begins to exist with zoogleic form; Increase along with the time, microorganism conforms gradually and constantly increases and breed, therefore move about the 3rd week at reactor, microbial film thickness obviously increases, the filler outward appearance changes into light brown gradually, and protozoon and a spot of metazoan have appearred, dominant is the campanularian of anchorage, the ciliate of swimming property etc.; To the 4th week, the filler appearance color changes brown into, the microbial film that get filling surface this moment carries out microscopy, find that the biophase on the filler is very abundant, mainly contain campanularian, tired branch worm, wheel animalcule, heliozooid, ciliate, nematode etc., the structure of community composition tends towards stability, and illustrates that the situ bio-contact oxidation system operation situation is good.

Observed as can be known by biophase, the biology on the microbial film is developed to senior gradually by rudimentary.In system's operational process, poor nutrition aerobic denitrifying bacteria HY1 group conforms gradually, is able to growth and breeding and keeps certain quantity on biologic packing material.Bacterium, zoogloea, protozoon and metazoan have been formed a metastable structure of community, and are bringing into play effect separately.Activated bacterial plays the metabolic subjective role of life, zoogloea has very strong adsorptive power and capacity of decomposition, for protozoon and miniature metazoan provide good living environment and have adhered to the place, protozoons such as campanularian are made a living to engulf bacterium, impel bacterium constantly to breed, miniature metazoan such as nematode serves as the active thing with the food carrion mainly, plays softening microbial film, impels the effect of biofilm detachment, thereby make microbial film keep good activity and purification function.Figure 7 shows that the part microorganism photo on the filler, the magnification of each figure is 40 * 10 times.Wherein figure (a) is a wheel animalcule, and figure (b) is a campanularian, and figure (c) is a nematode.

Therefore, in system's operational process, the change procedure of translucent → faint yellow → light brown → brown that the microbial film outward appearance on the filler presents white, a series of variations also take place in the biology on the filler, the dominant animal kind indicates biomembranous maturation if any campanularian, tired branch worm, wheel animalcule, heliozooid, ciliate, nematode etc.

In sum, the method for situ bio-contact oxidation restoring micro-scale polluted reservoir water of the present invention is the effective way of improving micro-polluted source water water quality, to the COD in the water_Mn, ammonia nitrogen, nitre nitrogen, total nitrogen and turbidity all have preferably treatment effect, maximum material removal rate is respectively 25.3%, 75%, 80%, 77.6% and 90.3%. Ammonia nitrogen during system stable operation and total nitrogen concentration all can reach the quality standard requirement of surface water environment III class water body. Compare with the other biological preliminary treatment as a kind of in-situ treatment, need not new construction of structures, can effectively remove pollutant in water again, have vast potential for future development.

Claims (2)

1. microorganism that is used for restoring micro-scale polluted reservoir water, it is characterized in that, described microorganism is nitrobacteria SY1 (Pseudomonas sp.SY1), deposit number is: CCTCCNO:M 209180 and denitrifying bacterium HY1 (Pseudomonas sp.HY1), deposit number is: CCTCCNO:M 209181.

2. a method that adopts the situ bio-contact oxidation restoring micro-scale polluted reservoir water is characterized in that, this method comprises the following steps:

1) select biologic packing material as microorganism carrier;

2) preparation of bacteria agent HS:

Described nitrobacteria SY1 (the Pseudomonas sp.SY1 of claim 1 with the screening acquisition, deposit number is: CCTCCNO:M 209180) and denitrifying bacterium HY1 (Pseudomonas sp.HY1, deposit number is: CCTCCNO:M 209181) be inoculated in the HS enrichment medium, after 2-3d is cultivated in the constant temperature vibration, bacterial classification is transplanted enlarged culturing in the HSK substratum, and 2d is cultivated in the constant temperature vibration;

The prescription of described HS enrichment medium is: NaAc 0.5g, NaNO ₃0.1g, NH ₄Cl 0.1g, MgSO ₄0.02g, CaCl ₂0.02g, K ₂HPO ₄0.05g, distilled water 1L, pH transfers to 7-7.5;

The prescription of described HSK substratum is: NaAc 0.1g, NaNO ₃0.02g, NH ₄Cl 0.02g, K ₂HPO ₄0.01g, sterilization source water 0.3-0.4L, distilled water is settled to 1L, and pH transfers to 7-7.5;

3) microorganism colonization: place the microbial inoculum of usefulness " HSK substratum " enlarged culturing to carry out bio-film colonization the biologic packing material of selecting, bio-film colonization is controlled at 2-3mg/L by aerating apparatus with the concentration of DO, and every day, the nutrient solution with 30-40% was changed to pending source water, reduce nutrient concentrations gradually, make microorganism nitrobacteria SY1 in the biologic packing material and denitrifying bacterium HY1 adapt to the water quality condition of micro-polluted source water, continuous aeration biofilm 4-5d, treat that biological filler forms that biofilm finishes behind the yellow microbial film, adopt solid stream of water to wash 4～5 times continuously, wash out the bacterium of the absorption of filling surface, make the biological fixnig filler;

4) situ bio-contact oxidation restoring micro-scale polluted reservoir water: the biological fixnig filler for preparing is connected and fixed on around the aerator that pumps up water with rope, adopts and pump up water aeration-situ bio-contact oxidation to carrying out biological restoration in the micro-polluted source water.

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