CN102633369A - Method for repairing micro-polluted water body by utilizing bottom mud biological agent - Google Patents

Method for repairing micro-polluted water body by utilizing bottom mud biological agent Download PDF

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Publication number
CN102633369A
CN102633369A CN2012101110476A CN201210111047A CN102633369A CN 102633369 A CN102633369 A CN 102633369A CN 2012101110476 A CN2012101110476 A CN 2012101110476A CN 201210111047 A CN201210111047 A CN 201210111047A CN 102633369 A CN102633369 A CN 102633369A
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bed mud
water
administer
nutrient medium
micropollutant
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CN2012101110476A
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CN102633369B (en
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苏俊峰
黄廷林
王旭冕
卢金锁
魏巍
袁杰
智利
黄文斌
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Xian University of Architecture and Technology
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Xian University of Architecture and Technology
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Abstract

The invention discloses a method for repairing a micro-polluted water body by utilizing a bottom mud biological agent. The method comprises the following steps of: firstly enriching acclimatized heterotrophic nitrifiers by utilizing a manner of gradually reducing the concentration of nutrients to enable the heterotrophic nitrifiers to be dominant flora; secondly enriching acclimatized aerobic denitrifying bacteria by utilizing the manner of gradually reducing the concentration of the nutrients to enable the aerobic denitrifying bacteria to be the dominant flora; thirdly compounding acclimatized nitrified and denitrified bottom mud according to different water qualities to obtain the bottom mud biological agent; and finally injecting the prepared bottom mud biological agent into the micro-polluted water body, and carrying out in-situ repair on the micro-polluted water body by utilizing the bottom mud biological agent. According to the method for repairing the micro-polluted water body by utilizing the bottom mud biological agent, disclosed by the invention, the utilized biological agent bacteria source is from water body bottom mud to be treated, the adaptive capacity to environment is strong, and a treatment effect is good; carbon removal and denitrification can be synchronized, the water quality of the micro-polluted water body is modified, and water body prevents from generating eutrophication; simultaneously, the method disclosed by the invention has excellent biological safety and low ecological risk.

Description

A kind of method of utilizing the bed mud biotechnological formulation to repair micropollutant water
Technical field
The present invention relates to sewage disposal technology, be specifically related to a kind of method of utilizing the bed mud biotechnological formulation to repair micropollutant water.
Background technology
In recent years; China lake and reservoir eutrophication problem are serious day by day; Only the water source reservoir (lake) public reported in 2006 and 2007 just has tens because eutrophication problem has a strong impact on the example of Urban water supply, and the blue-green algae of taking place like water source Hu Ku such as Taihu Lake, Wuxi, Changchun Xinlichen Reservoir, the Tashan Mountain, Ganyu County, Jiangsu reservoir, Tongchuan, Shaanxi Liu Wan reservoir, Chaohu, Anhui, the Gangnan Reservoir He Huangbi village, Shijiazhuang reservoir, Feng huangshan Mountain, Zhuhai reservoirs breaks out and the body eutrophication problem.In 28 state control emphasis lakes (storehouse) that 2008 years were detected, meet 4 of II class water quality, account for 14.3%; 2 of III class account for 7.1%; 6 of IV class account for 21.4%; 5 of V class account for 17.9%; 11 of bad V class account for 39.3%.Main contamination index is total nitrogen and total phosphorus.In 26 lakes (storehouse) of monitoring nutritional status, eutrophic 1 of severe accounts for 3.8%; Eutrophic 5 of moderate accounts for 19.2%; Slight eutrophic 6, account for 23.0%.
A large amount of breedings of reservoir eutrophication and algae cause that organism, colourity etc. increase substantially in the water, and it is unusual to smell flavor; Algae and excretory organism thereof have increased the water treatment difficulty, have improved the growing amount of DBPs; Blue-green alga bloom has increased the burst size of the very strong algae toxin of toxicity simultaneously.In recent years; Begin from water body, to separate the efficient denitrification bacterium both at home and abroad and process bacteria agent; Through throwing bacterium ruling by law reason body eutrophication and having obtained certain achievement; Throwing the bacterium method is to add certain useful microbe in polluted-water, reduces or eliminates water pollution, promotes the recovery of water ecology function.In contrast to traditional treatment process, advantages such as processing costs is low, easy and simple to handle, obvious processing effect that it has.But in micro-polluted water, add bacteria agent and can produce ecological risk, and external microbial inoculum is difficult in the environment that the short period of time adapts to source water and causes treatment effect to descend.
Summary of the invention
To the defective or the deficiency of prior art, the object of the invention is to provide a kind of method of utilizing the bed mud biotechnological formulation to repair micropollutant water.With under the prerequisite of not bringing foreign bacteria, effectively solve pollutents such as micropollutant water COD, N, P and exceed standard, body eutrophication frequently takes place, and reduces the water body using value.
For realizing above-mentioned technical assignment, the present invention takes following technical solution:
A kind of method of utilizing the bed mud biotechnological formulation to repair micropollutant water is characterized in that method is undertaken by following step:
Step 1, nitrated bed mud preparation: get bed mud the micropollutant water from waiting to administer, getting bed mud is cultivated domestication 15 days under 25 ℃ in liquid nutrient medium, obtain nitrated bed mud, wherein the volume ratio of bed mud and liquid nutrient medium is: 1: (10~20);
Cultivate liquid nutrient medium of replacing in per three days in the domestication process; Employed liquid nutrient medium is different during each the replacing, and used liquid medium is followed successively by according to changing order: the water distribution nutrient solution and the sterilization of 1 volume that the water distribution nutrient solution of water distribution nutrient solution, 3 volumes and the sterilization of 1 volume wait to administer liquid nutrient medium that micropollutant water forms, 2 volumes waited to administer liquid nutrient medium, the water distribution nutrient solution of 1 volume and the sterilization of 1 volume that micropollutant water forms and waited to administer the liquid nutrient medium of micropollutant water composition, the micropollutant water to be administered of sterilizing;
Step 2: denitrification bed mud preparation: get bed mud the micropollutant water from waiting to administer, with cultivating domestication 15 days in the getting bed mud liquid medium within, obtain the denitrification bed mud, wherein the volume ratio of bed mud and liquid nutrient medium is: 1: (10~20);
Cultivate in the domestication process every day and change liquid nutrient medium one time, used liquid nutrient medium was in first day~the 3rd day: water distribution nutrient solution, the 4th day~the 6th day used liquid nutrient medium are: the water distribution nutrient solution of 3 volumes and the sterilization of 1 volume are waited to administer liquid nutrient medium, the 7th day~the 9th day used liquid nutrient medium that micropollutant water forms and are: the water distribution nutrient solution of 2 volumes and the sterilization of 1 volume wait to administer the micropollutant water composition liquid nutrient medium, the tenth day~used liquid nutrient medium was in the 12 day: the water distribution nutrient solution of 1 volume and the sterilization of 1 volume wait that liquid nutrient medium, the 13 day~the 15 day used liquid nutrient medium of administering the micropollutant water composition are: the micropollutant water to be administered of sterilizing;
The prescription of the water distribution nutrient solution described in step 1 and the step 2 is:
Sodium acetate: 0.3g,
NH 4Cl:0.05g,
KH 2PO 4:0.02g,
MgSO 4·7H 2O:0.01g,
CaCl 2:0.01g,
Trace element solution: 2ml,
Zero(ppm) water: 1000mL;
Said trace element solution is: in mass concentration, and 0.5g/L EDTA, 0.1g/L ZnSO 4, 0.05g/LMnCl 24H 2O, 0.1g/LFeSO 47H 2O, 0.1g/LCuSO 45H 2O, 0.05g/LCoCl 26H 2The aqueous solution of O;
Step 3, according to waiting that the water quality of administering micropollutant water prepares the bed mud biotechnological formulation:
When the ammonia nitrogen of waiting to administer micropollutant water accounts for the mass percent of total nitrogen>80%, the nitrated bed mud of 2 weight parts must be mixed bed mud with the denitrification bed mud of 1 weight part is mixed;
When the mass percent that accounts for total nitrogen when the ammonia nitrogen of waiting to administer micropollutant water is 50%~80%, the nitrated bed mud of 1 weight part must be mixed bed mud with the denitrification bed mud of 1 weight part is mixed;
When the mass percent that accounts for total nitrogen when the nitre nitrogen of waiting to administer micropollutant water is 50%~80%, the nitrated bed mud of 1 weight part must be mixed bed mud with the denitrification bed mud of 2 weight parts is mixed;
When the nitre nitrogen of waiting to administer micropollutant water accounts for the mass percent of total nitrogen>80%, with the denitrification bed mud as mixing bed mud;
Then the mixing bed mud of 1 volume and the sterilization of 10 volumes are waited to administer the mixed bed mud biotechnological formulation that gets of micropollutant water;
Step 4: the bed mud biotechnological formulation is added to waits to administer in the micropollutant water, treat and administer micropollutant water and carry out the biology in situ reparation, the volume of the bed mud biotechnological formulation that is added be wait to administer 1,000,000 of micropollutant water volume/.
Bed mud biotechnological formulation of the present invention is repaired micropollutant water techniques make use bed mud as the bacterium source, obtains biotechnological formulation through the bed mud domestication, repairs micropollutant water through the mode original position that biotechnological formulation is delivered directly.Biotechnological formulation bacterium source used in the method comes from the staying water bed mud, and strong to adaptive capacity to environment, treatment effect is good; Take this method carbon and nitrogen removal synchronously, improve micropollutant water water quality, control water body generation eutrophication; While method has good biological safety, and ecological risk is low.
Description of drawings
Fig. 1 is among the embodiment 1 during different biotechnological formulation dosage, the comparative analysis graphic representation of ammonia nitrogen removal effect;
Fig. 2 is among the embodiment 1 during different biotechnological formulation dosage, the comparative analysis graphic representation of total nitrogen removal effect;
Fig. 3 be among the embodiment 2 biotechnological formulation to the removal effect graphic representation of micropollutant water ammonia nitrogen;
Fig. 4 be among the embodiment 2 bacteria agent to the removal effect graphic representation of micropollutant water total nitrogen.
Below in conjunction with embodiment and accompanying drawing the present invention is done further explain.
Embodiment
Below be the specific embodiment that the contriver provides, need to prove that the embodiment that is provided further explains to of the present invention, protection scope of the present invention is not limited to the embodiment that provided.
Embodiment 1:
Micropollutant water to be administered among this embodiment is: Xi'an Datang lotus landscape is seen water body; Defer to technical scheme of the present invention; Characteristics according to micropollutant water water quality to be administered; The nitrated bed mud of 2 weight parts must be mixed bed mud with the denitrification bed mud of 1 weight part is mixed, and the volume of the bed mud biotechnological formulation that adds is to wait that there is regulation effect preferably in the 1000000/time of administering the micropollutant water volume, can find out from Fig. 1 and Fig. 2; The bed mud biotechnological formulation is relatively poor to the removal effect of ammonia nitrogen and total nitrogen during 1,/10,000,000 throwing amount; And the removal effect difference of the 1/10 ten thousand and 1/,100 ten thousand pair of ammonia nitrogen and total nitrogen is little, considers 1/,100 ten thousand throwing amount bed mud biotechnological formulation consumption still less, and the dosage of practical application adopts 1/1,000,000 throwing amounts.The 5th day ammonia-N removal rate of 1/,100 ten thousand throwing amount cultured continuously is near 90%; Ammonia nitrogen removal frank reached 93.23% in the 9th day; The clearance of total nitrogen reaches 47.79%; Reach 51.1% and contrast the 9th day ammonia nitrogen removal frank, the clearance of total nitrogen is merely 12.88%, and bacteria agent shows the effect of biological denitrificaion preferably.
Embodiment 2:
Micro-polluted source water to be administered among this embodiment is: Xi'an dan stone acupuncture needle valley reservoir, defer to technical scheme of the present invention, according to water quality characteristics only with the denitrification bed mud as biotechnological formulation; The volume of the bed mud biotechnological formulation that adds is to wait that there is regulation effect preferably in the 1000000/time of administering the micropollutant water volume; Can find out that from Fig. 3 and Fig. 4 through the METHOD FOR CONTINUOUS DETERMINATION of 15d, the bed mud biotechnological formulation can obviously be accelerated the nitrifying process of water body; Final ammonia nitrogen removal frank reaches 96.84%; Though the final ammonia nitrogen removal frank of contrast is also more than 90%, nitrifying process lags behind the clearance of bed mud biotechnological formulation, and the bed mud biotechnological formulation reaches 39.45% to the clearance of total nitrogen; Contrast only has 14.64% to the clearance of total nitrogen is the highest, and the bed mud biotechnological formulation shows Nitrogen removal effect preferably.

Claims (1)

1. method of utilizing the bed mud biotechnological formulation to repair micropollutant water is characterized in that method is undertaken by following step:
Step 1, nitrated bed mud preparation: get bed mud the micropollutant water from waiting to administer, getting bed mud is cultivated domestication 15 days under 25 ℃ in liquid nutrient medium, obtain nitrated bed mud, wherein the volume ratio of bed mud and liquid nutrient medium is: 1: (10~20);
Cultivate liquid nutrient medium of replacing in per three days in the domestication process; Employed liquid nutrient medium is different during each the replacing, and used liquid medium is followed successively by according to changing order: the water distribution nutrient solution and the sterilization of 1 volume that the water distribution nutrient solution of water distribution nutrient solution, 3 volumes and the sterilization of 1 volume wait to administer liquid nutrient medium that micropollutant water forms, 2 volumes waited to administer liquid nutrient medium, the water distribution nutrient solution of 1 volume and the sterilization of 1 volume that micropollutant water forms and waited to administer the liquid nutrient medium of micropollutant water composition, the micropollutant water to be administered of sterilizing;
Step 2: denitrification bed mud preparation: get bed mud the micropollutant water from waiting to administer, with cultivating domestication 15 days in the getting bed mud liquid medium within, obtain the denitrification bed mud, wherein the volume ratio of bed mud and liquid nutrient medium is: 1: (10~20);
Cultivate in the domestication process every day and change liquid nutrient medium one time, used liquid nutrient medium was in first day~the 3rd day: water distribution nutrient solution, the 4th day~the 6th day used liquid nutrient medium are: the water distribution nutrient solution of 3 volumes and the sterilization of 1 volume are waited to administer liquid nutrient medium, the 7th day~the 9th day used liquid nutrient medium that micropollutant water forms and are: the water distribution nutrient solution of 2 volumes and the sterilization of 1 volume wait to administer the micropollutant water composition liquid nutrient medium, the tenth day~used liquid nutrient medium was in the 12 day: the water distribution nutrient solution of 1 volume and the sterilization of 1 volume wait that liquid nutrient medium, the 13 day~the 15 day used liquid nutrient medium of administering the micropollutant water composition are: the micropollutant water to be administered of sterilizing;
The prescription of the water distribution nutrient solution described in step 1 and the step 2 is:
Sodium acetate: 0.3g,
NH 4Cl:0.05g,
KH 2PO 4:0.02g,
MgSO 4·7H 2O:0.01g,
CaCl 2:0.01g,
Trace element solution: 2ml,
Zero(ppm) water: 1000mL;
Said trace element solution is: in mass concentration, and 0.5g/L EDTA, 0.1g/L ZnSO 4, 0.05g/LMnCl 24H 2O, 0.1g/LFeSO 47H 2O, 0.1g/LCuSO 45H 2O, 0.05g/LCoCl 26H 2The aqueous solution of O;
Step 3, according to waiting that the water quality of administering micropollutant water prepares the bed mud biotechnological formulation:
When the ammonia nitrogen of waiting to administer micropollutant water accounts for the mass percent of total nitrogen>80%, the nitrated bed mud of 2 weight parts must be mixed bed mud with the denitrification bed mud of 1 weight part is mixed;
When the mass percent that accounts for total nitrogen when the ammonia nitrogen of waiting to administer micropollutant water is 50%~80%, the nitrated bed mud of 1 weight part must be mixed bed mud with the denitrification bed mud of 1 weight part is mixed;
When the mass percent that accounts for total nitrogen when the nitre nitrogen of waiting to administer micropollutant water is 50%~80%, the nitrated bed mud of 1 weight part must be mixed bed mud with the denitrification bed mud of 2 weight parts is mixed;
When the nitre nitrogen of waiting to administer micropollutant water accounts for the mass percent of total nitrogen>80%, with the denitrification bed mud as mixing bed mud;
Then the mixing bed mud of 1 volume and the sterilization of 10 volumes are waited to administer the mixed bed mud biotechnological formulation that gets of micropollutant water;
Step 4: the bed mud biotechnological formulation is added to waits to administer in the micropollutant water, treat and administer micropollutant water and carry out the biology in situ reparation, the volume of the bed mud biotechnological formulation that is added be wait to administer 1,000,000 of micropollutant water volume/.
CN 201210111047 2012-04-17 2012-04-17 Method for repairing micro-polluted water body by utilizing bottom mud biological agent Expired - Fee Related CN102633369B (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104862257A (en) * 2015-06-02 2015-08-26 湖北工业大学 Enrichment culture method of indigenous nitrogen transforming microorganism and application of same to treatment of water ammonia-nitrogen pollution
CN107058207A (en) * 2017-04-19 2017-08-18 大连理工大学 A kind of rejuvenation method of the heterotrophic nitrification aerobic denitrifying bacterium for bisphenols nitric wastewater of degrading
CN111576339A (en) * 2020-05-12 2020-08-25 中国城市建设研究院有限公司 Ecological revetment for strengthening treatment of black and odorous water body by microorganisms
CN113044962A (en) * 2021-03-11 2021-06-29 西安建筑科技大学 Ecological water restoration method based on biological aggregation membrane
CN114262134A (en) * 2021-12-22 2022-04-01 西安建筑科技大学 Method for realizing in-situ sludge reduction by using sludge biological agent

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US20020162794A1 (en) * 2001-02-27 2002-11-07 Tock Richard William Anaerobic digestion
CN101050043A (en) * 2007-05-11 2007-10-10 胡法 Ecological management method for water pollution
CN101555088A (en) * 2009-05-15 2009-10-14 李华林 Method for treating black-odor rivers and lakes with microbiological preparations
CN101691547A (en) * 2009-09-29 2010-04-07 西安建筑科技大学 Method for restoring micro-scale polluted reservoir water by using situ bio-contact oxidation

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020162794A1 (en) * 2001-02-27 2002-11-07 Tock Richard William Anaerobic digestion
CN101050043A (en) * 2007-05-11 2007-10-10 胡法 Ecological management method for water pollution
CN101555088A (en) * 2009-05-15 2009-10-14 李华林 Method for treating black-odor rivers and lakes with microbiological preparations
CN101691547A (en) * 2009-09-29 2010-04-07 西安建筑科技大学 Method for restoring micro-scale polluted reservoir water by using situ bio-contact oxidation

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104862257A (en) * 2015-06-02 2015-08-26 湖北工业大学 Enrichment culture method of indigenous nitrogen transforming microorganism and application of same to treatment of water ammonia-nitrogen pollution
CN104862257B (en) * 2015-06-02 2018-01-12 湖北工业大学 A kind of method of indigenous nitrogen microbial enrichment culture and its application in ammonia nitrogen pollution in water body improvement
CN107058207A (en) * 2017-04-19 2017-08-18 大连理工大学 A kind of rejuvenation method of the heterotrophic nitrification aerobic denitrifying bacterium for bisphenols nitric wastewater of degrading
CN111576339A (en) * 2020-05-12 2020-08-25 中国城市建设研究院有限公司 Ecological revetment for strengthening treatment of black and odorous water body by microorganisms
CN113044962A (en) * 2021-03-11 2021-06-29 西安建筑科技大学 Ecological water restoration method based on biological aggregation membrane
CN113044962B (en) * 2021-03-11 2024-01-16 西安建筑科技大学 Ecological water restoration method based on biological aggregation membrane
CN114262134A (en) * 2021-12-22 2022-04-01 西安建筑科技大学 Method for realizing in-situ sludge reduction by using sludge biological agent

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