CN102633369B - Method for repairing micro-polluted water body by utilizing bottom mud biological agent - Google Patents
Method for repairing micro-polluted water body by utilizing bottom mud biological agent Download PDFInfo
- Publication number
- CN102633369B CN102633369B CN 201210111047 CN201210111047A CN102633369B CN 102633369 B CN102633369 B CN 102633369B CN 201210111047 CN201210111047 CN 201210111047 CN 201210111047 A CN201210111047 A CN 201210111047A CN 102633369 B CN102633369 B CN 102633369B
- Authority
- CN
- China
- Prior art keywords
- bed mud
- water
- nutrient medium
- administered
- liquid nutrient
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a method for repairing a micro-polluted water body by utilizing a bottom mud biological agent. The method comprises the following steps of: firstly enriching acclimatized heterotrophic nitrifiers by utilizing a manner of gradually reducing the concentration of nutrients to enable the heterotrophic nitrifiers to be dominant flora; secondly enriching acclimatized aerobic denitrifying bacteria by utilizing the manner of gradually reducing the concentration of the nutrients to enable the aerobic denitrifying bacteria to be the dominant flora; thirdly compounding acclimatized nitrified and denitrified bottom mud according to different water qualities to obtain the bottom mud biological agent; and finally injecting the prepared bottom mud biological agent into the micro-polluted water body, and carrying out in-situ repair on the micro-polluted water body by utilizing the bottom mud biological agent. According to the method for repairing the micro-polluted water body by utilizing the bottom mud biological agent, disclosed by the invention, the utilized biological agent bacteria source is from water body bottom mud to be treated, the adaptive capacity to environment is strong, and a treatment effect is good; carbon removal and denitrification can be synchronized, the water quality of the micro-polluted water body is modified, and water body prevents from generating eutrophication; simultaneously, the method disclosed by the invention has excellent biological safety and low ecological risk.
Description
Technical field
The present invention relates to sewage disposal technology, be specifically related to a kind of method of utilizing the bed mud biotechnological formulation to repair micropollutant water.
Background technology
In recent years, China lake and Reservoir Eutrophication problem are day by day serious, only in 2006 and 2007 the water source reservoir (lake) of open report because the example that eutrophication problem has a strong impact on Urban water supply just has tens, the Blue-green Algae Fast Growth and the body eutrophication problem that occur as water source Hu Ku such as Taihu Lake, Wuxi, Changchun Xinlichen Reservoir, Ganyu County, Jiangsu Tashan Reservoir, Tongchuan, Shaanxi Province willow gulf reservoir, Chaohu, Anhui Province, China, the Gangnan Reservoir He Huangbi village, Shijiazhuang reservoir, Feng huangshan Mountain, Zhuhai reservoirs.In 28 state control emphasis lakes (storehouse) that 2008 years were detected, meet 4 of II class water quality, account for 14.3%; 2 of III class account for 7.1%; 6 of IV class account for 21.4%; 5 of V class account for 17.9%; 11 of bad V class account for 39.3%.Main contamination index is total nitrogen and total phosphorus.In 26 lakes (storehouse) of monitoring nutritional status, eutrophic 1 of severe accounts for 3.8%; Eutrophic 5 of moderate accounts for 19.2%; Slight eutrophic 6, account for 23.0%.
The amount reproduction of Reservoir Eutrophication and algae causes that in water, organism, colourity etc. increase substantially, and smells flavor abnormal; The organism of algae and secretion thereof has increased the water treatment difficulty, has improved the growing amount of disinfection byproduct (DBP); Blue-green alga bloom has increased the burst size of the very strong algae toxin of toxicity simultaneously.In recent years, begin to separate the efficient denitrification bacterium both at home and abroad and make bacteria agent from water body, by throwing bacterium ruling by law reason body eutrophication and having obtained certain achievement, throwing the bacterium method is to add certain beneficial microorganism in polluted-water, reduce or eliminate water pollution, promote the recovery of water ecology function.In contrast to traditional treatment process, the advantages such as processing costs is low, easy and simple to handle, obvious processing effect that it has.But add bacteria agent in the micro-polluted water and can produce ecological risk, and the environment that external microbial inoculum is difficult to adapt in the short period of time source water causes treatment effect to descend.
Summary of the invention
For defective or the deficiency of prior art, the object of the invention is to provide a kind of method of utilizing the bed mud biotechnological formulation to repair micropollutant water.With under the prerequisite of not bringing foreign bacteria, effectively solve the pollutents such as micropollutant water COD, N, P and exceed standard, body eutrophication frequently occurs, and reduces the water body using value.
For realizing above-mentioned technical assignment, the present invention takes following technical solution:
A kind of method of utilizing the bed mud biotechnological formulation to repair micropollutant water is characterized in that, method is carried out in the steps below:
Cultivate liquid nutrient medium of replacing in every three days in the domestication process, the liquid nutrient medium that uses during each the replacing is different, and liquid medium used is followed successively by according to changing order: liquid nutrient medium, the micropollutant water to be administered of sterilizing that the micropollutant water to be administered of sterilizing of the liquid nutrient medium that the micropollutant water to be administered of sterilizing of the liquid nutrient medium that the micropollutant water to be administered of sterilizing of the water distribution nutrient solution of water distribution nutrient solution, 3 volumes and 1 volume forms, the water distribution nutrient solution of 2 volumes and 1 volume forms, the water distribution nutrient solution of 1 volume and 1 volume forms;
Step 2: denitrification bed mud preparation: get bed mud from micropollutant water to be administered, with cultivating domestication 15 days in getting bed mud liquid medium within, obtain the denitrification bed mud, wherein the volume ratio of bed mud and liquid nutrient medium is: 1: (10～20);
cultivate in the domestication process and to change one time liquid nutrient medium every day, first day～3rd a day liquid nutrient medium used is: the water distribution nutrient solution, liquid nutrient medium used was in the 4th day～the 6th day: the liquid nutrient medium that the micropollutant water to be administered of sterilizing of the water distribution nutrient solution of 3 volumes and 1 volume forms, liquid nutrient medium used was in the 7th day～the 9th day: the liquid nutrient medium that the micropollutant water to be administered of sterilizing of the water distribution nutrient solution of 2 volumes and 1 volume forms, the tenth day～liquid nutrient medium used was in the 12 day: the liquid nutrient medium that the micropollutant water to be administered of sterilizing of the water distribution nutrient solution of 1 volume and 1 volume forms, liquid nutrient medium used was in the 13 day～the 15 day: the micropollutant water to be administered of sterilizing,
The formula of the water distribution nutrient solution described in step 1 and step 2 is:
Sodium acetate: 0.3g,
NH
4Cl：0.05g，
KH
2PO
4：0.02g，
MgSO
4·7H
2O：0.01g，
CaCl
2：0.01g，
Trace element solution: 2ml,
Distilled water: 1000mL;
Described trace element solution is: in mass concentration, and 0.5g/L EDTA, 0.1g/L ZnSO
4, 0.05g/LMnCl
24H
2O, 0.1g/LFeSO
47H
2O, 0.1g/LCuSO
45H
2O, 0.05g/LCoCl
26H
2The aqueous solution of O;
When the mass percent that accounts for total nitrogen wait the ammonia nitrogen of administering micropollutant water＞80%, the nitrated bed mud of 2 weight parts is mixed making the mixing bed mud with the denitrification bed mud of 1 weight part;
When the mass percent that accounts for total nitrogen wait the ammonia nitrogen of administering micropollutant water is 50%～80%, the nitrated bed mud of 1 weight part is mixed making the mixing bed mud with the denitrification bed mud of 1 weight part;
When the mass percent that accounts for total nitrogen wait the nitre nitrogen of administering micropollutant water is 50%～80%, the nitrated bed mud of 1 weight part is mixed making the mixing bed mud with the denitrification bed mud of 2 weight parts;
When the mass percent that accounts for total nitrogen wait the nitre nitrogen of administering micropollutant water＞80%, with the denitrification bed mud as mixing bed mud;
Then the mixing bed mud of 1 volume is mixed with sterilizing of 10 volumes micropollutant water to be administered and make the bed mud biotechnological formulation;
Step 4: the bed mud biotechnological formulation is added in micropollutant water to be administered, treats and administer micropollutant water and carry out the biology in situ reparation, the volume of the bed mud biotechnological formulation that adds be 1,000,000 of micropollutant water volume to be administered/.
Bed mud biotechnological formulation of the present invention is repaired the micro-polluted water body technique and is utilized bed mud as the bacterium source, tames by bed mud and obtains biotechnological formulation, repairs micropollutant water by the mode original position that biotechnological formulation is delivered directly.In method, biotechnological formulation bacterium used source comes from the staying water bed mud, and strong to adaptive capacity to environment, treatment effect is good; Take synchronously carbon and nitrogen removal of the method, improve micropollutant water water quality, control water body generation eutrophication; While method has good biological safety, and ecological risk is low.
Description of drawings
Fig. 1 is in embodiment 1 during different biotechnological formulation dosage, the comparative analysis graphic representation of ammonia nitrogen removal effect;
Fig. 2 is in embodiment 1 during different biotechnological formulation dosage, the comparative analysis graphic representation of total nitrogen removal effect;
Fig. 3 is the removal effect graphic representation of biotechnological formulation to the micropollutant water ammonia nitrogen in embodiment 2;
Fig. 4 is the removal effect graphic representation of bacteria agent to the micropollutant water total nitrogen in embodiment 2.
Below in conjunction with embodiment and accompanying drawing, the present invention is described in further detail.
Embodiment
Be below the specific embodiment that the contriver provides, need to prove, the embodiment that provides further explains to of the present invention, the embodiment that protection scope of the present invention is not limited to provide.
Embodiment 1:
micropollutant water to be administered in this embodiment is: Xi'an Datang lotus landscape is seen water body, defer to technical scheme of the present invention, characteristics according to micropollutant water water quality to be administered, the nitrated bed mud of 2 weight parts is mixed with the denitrification bed mud of 1 weight part make the mixing bed mud, the volume of the bed mud biotechnological formulation that adds is wait the 1000000/time of administering the micropollutant water volume, regulation effect preferably to be arranged, can find out from Fig. 1 and Fig. 2, during 1,/10,000,000 throwing amount, the bed mud biotechnological formulation is relatively poor to the removal effect of ammonia nitrogen and total nitrogen, and the removal effect difference of the 1/10 ten thousand and 1/,100 ten thousand pair of ammonia nitrogen and total nitrogen is little, consider 1/,100 ten thousand throwing amount bed mud biotechnological formulation consumption still less, the dosage of practical application adopts 1/,100 ten thousand throwing amount.The clearance of the 5th day ammonia nitrogen of 1/,100 ten thousand throwing amount cultured continuously is near 90%, ammonia nitrogen removal frank reached 93.23% in the 9th day, the clearance of total nitrogen reaches 47.79%, reach 51.1% and contrast the 9th day ammonia nitrogen removal frank, the clearance of total nitrogen is only 12.88%, and bacteria agent shows the effect of biological denitrificaion preferably.
Embodiment 2:
micro-polluted source water to be administered in this embodiment is: the Xi'an dan is performed acupunctures with stone needles the valley reservoir, defer to technical scheme of the present invention, according to water quality characteristics only with the denitrification bed mud as biotechnological formulation, the volume of the bed mud biotechnological formulation that adds is wait the 1000000/time of administering the micropollutant water volume, regulation effect preferably to be arranged, can find out from Fig. 3 and Fig. 4, METHOD FOR CONTINUOUS DETERMINATION through 15d, the bed mud biotechnological formulation can obviously be accelerated the nitrifying process of water body, final ammonia nitrogen removal frank reaches 96.84%, although the final ammonia nitrogen removal frank of contrast is also more than 90%, but nitrifying process lags behind the clearance of bed mud biotechnological formulation, the bed mud biotechnological formulation reaches 39.45% to the clearance of total nitrogen, contrast only has 14.64% to the clearance of total nitrogen is the highest, the bed mud biotechnological formulation shows Nitrogen removal effect preferably.
Claims (1)
1. a method of utilizing the bed mud biotechnological formulation to repair micropollutant water, is characterized in that, method is carried out in the steps below:
Step 1, nitrated bed mud preparation: get bed mud from micropollutant water to be administered, getting bed mud is cultivated domestication 15 days under 25 ℃ in liquid nutrient medium, obtain nitrated bed mud, wherein the volume ratio of bed mud and liquid nutrient medium is: 1: (10～20);
Cultivate liquid nutrient medium of replacing in every three days in the domestication process, the liquid nutrient medium that uses during each the replacing is different, and liquid nutrient medium used is followed successively by according to changing order: liquid nutrient medium, the micropollutant water to be administered of sterilizing that the micropollutant water to be administered of sterilizing of the liquid nutrient medium that the micropollutant water to be administered of sterilizing of the liquid nutrient medium that the micropollutant water to be administered of sterilizing of the water distribution nutrient solution of water distribution nutrient solution, 3 volumes and 1 volume forms, the water distribution nutrient solution of 2 volumes and 1 volume forms, the water distribution nutrient solution of 1 volume and 1 volume forms;
Step 2: denitrification bed mud preparation: get bed mud from micropollutant water to be administered, with cultivating domestication 15 days in getting bed mud liquid medium within, obtain the denitrification bed mud, wherein the volume ratio of bed mud and liquid nutrient medium is: 1: (10～20);
cultivate in the domestication process and to change one time liquid nutrient medium every day, first day～3rd a day liquid nutrient medium used is: the water distribution nutrient solution, liquid nutrient medium used was in the 4th day～the 6th day: the liquid nutrient medium that the micropollutant water to be administered of sterilizing of the water distribution nutrient solution of 3 volumes and 1 volume forms, liquid nutrient medium used was in the 7th day～the 9th day: the liquid nutrient medium that the micropollutant water to be administered of sterilizing of the water distribution nutrient solution of 2 volumes and 1 volume forms, the tenth day～liquid nutrient medium used was in the 12 day: the liquid nutrient medium that the micropollutant water to be administered of sterilizing of the water distribution nutrient solution of 1 volume and 1 volume forms, liquid nutrient medium used was in the 13 day～the 15 day: the micropollutant water to be administered of sterilizing,
The formula of the water distribution nutrient solution described in step 1 and step 2 is:
Sodium acetate: 0.3g,
NH
4Cl：0.05g，
KH
2PO
4：0.02g，
MgSO
4·7H
2O：0.01g，
CaCl
2：0.01g，
Trace element solution: 2ml,
Distilled water: 1000mL;
Described trace element solution is: in mass concentration, and 0.5g/L EDTA, 0.1g/L ZnSO
4, 0.05g/LMnCl
24H
2O, 0.1g/LFeSO
47H
2O, 0.1g/LCuSO
45H
2O, 0.05g/LCoCl
26H
2The aqueous solution of O;
Step 3 prepares the bed mud biotechnological formulation according to the water quality of micropollutant water to be administered:
When the mass percent that accounts for total nitrogen wait the ammonia nitrogen of administering micropollutant water＞80%, the nitrated bed mud of 2 weight parts is mixed making the mixing bed mud with the denitrification bed mud of 1 weight part;
When the mass percent that accounts for total nitrogen wait the ammonia nitrogen of administering micropollutant water is 50%～80%, the nitrated bed mud of 1 weight part is mixed making the mixing bed mud with the denitrification bed mud of 1 weight part;
When the mass percent that accounts for total nitrogen wait the nitre nitrogen of administering micropollutant water is 50%～80%, the nitrated bed mud of 1 weight part is mixed making the mixing bed mud with the denitrification bed mud of 2 weight parts;
When the mass percent that accounts for total nitrogen wait the nitre nitrogen of administering micropollutant water＞80%, with the denitrification bed mud as mixing bed mud;
Then the mixing bed mud of 1 volume is mixed with sterilizing of 10 volumes micropollutant water to be administered and make the bed mud biotechnological formulation;
Step 4: the bed mud biotechnological formulation is added in micropollutant water to be administered, treats and administer micropollutant water and carry out the biology in situ reparation, the volume of the bed mud biotechnological formulation that adds be 1,000,000 of micropollutant water volume to be administered/.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210111047 CN102633369B (en) | 2012-04-17 | 2012-04-17 | Method for repairing micro-polluted water body by utilizing bottom mud biological agent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210111047 CN102633369B (en) | 2012-04-17 | 2012-04-17 | Method for repairing micro-polluted water body by utilizing bottom mud biological agent |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102633369A CN102633369A (en) | 2012-08-15 |
| CN102633369B true CN102633369B (en) | 2013-05-22 |
Family
ID=46617971
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201210111047 Expired - Fee Related CN102633369B (en) | 2012-04-17 | 2012-04-17 | Method for repairing micro-polluted water body by utilizing bottom mud biological agent |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102633369B (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104862257B (en) * | 2015-06-02 | 2018-01-12 | 湖北工业大学 | A kind of method of indigenous nitrogen microbial enrichment culture and its application in ammonia nitrogen pollution in water body improvement |
| CN107058207A (en) * | 2017-04-19 | 2017-08-18 | 大连理工大学 | A kind of rejuvenation method of the heterotrophic nitrification aerobic denitrifying bacterium for bisphenols nitric wastewater of degrading |
| CN111576339B (en) * | 2020-05-12 | 2021-12-21 | 中国城市建设研究院有限公司 | Ecological revetment for strengthening treatment of black and odorous water body by microorganisms |
| CN113044962B (en) * | 2021-03-11 | 2024-01-16 | 西安建筑科技大学 | Ecological water restoration method based on biological aggregation membrane |
| CN114262134B (en) * | 2021-12-22 | 2023-01-17 | 西安建筑科技大学 | Method for realizing in-situ sludge reduction by using sludge biological agent |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101050043A (en) * | 2007-05-11 | 2007-10-10 | 胡法 | Ecological management method for water pollution |
| CN101555088A (en) * | 2009-05-15 | 2009-10-14 | 李华林 | Method for treating black-odor rivers and lakes with microbiological preparations |
| CN101691547A (en) * | 2009-09-29 | 2010-04-07 | 西安建筑科技大学 | Method for restoring micro-scale polluted reservoir water by using situ bio-contact oxidation |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020162794A1 (en) * | 2001-02-27 | 2002-11-07 | Tock Richard William | Anaerobic digestion |
-
2012
- 2012-04-17 CN CN 201210111047 patent/CN102633369B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101050043A (en) * | 2007-05-11 | 2007-10-10 | 胡法 | Ecological management method for water pollution |
| CN101555088A (en) * | 2009-05-15 | 2009-10-14 | 李华林 | Method for treating black-odor rivers and lakes with microbiological preparations |
| CN101691547A (en) * | 2009-09-29 | 2010-04-07 | 西安建筑科技大学 | Method for restoring micro-scale polluted reservoir water by using situ bio-contact oxidation |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102633369A (en) | 2012-08-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102633369B (en) | Method for repairing micro-polluted water body by utilizing bottom mud biological agent | |
| CN101367581B (en) | Composite microorganism formulation for controlling excessive multiplication of blue-green algae and preparation method thereof | |
| CN106277366B (en) | A kind of pig-breeding wastewater treatment microbial microbial inoculum and preparation method and application | |
| CN101434907A (en) | Microbial preparation for processing refuse leachate and preparation | |
| CN102701459B (en) | Plant root layered membrane-forming enhanced water body repairing method for biological denitrification | |
| CN105254004B (en) | A kind of cultural method for being resistant to sulfide anaerobic ammonium oxidation granular sludge culture | |
| CN105621611A (en) | Method for quickly starting short-cut nitrification and denitrification of ammonia-containing wastewater | |
| CN103373764B (en) | Method for quickly starting short-cut nitrification and denitrification of ammonia-containing wastewater | |
| CN103708610A (en) | Activated sludge cultivation method for coking wastewater treatment | |
| CN106635923A (en) | Method for preparing high-density salt-tolerant denitrifying bacterium agent applicable to wastewater treatment | |
| CN104692526B (en) | A kind of method that utilization goethitum improves wastewater treatment process denitrification denitrogenation speed | |
| CN103396947A (en) | Micro nutrient additive for microbial water treatment | |
| CN101948177B (en) | Method for enriching denitrifying phosphorus removal bacteria in continuous flow reactor | |
| CN103275908B (en) | Low-temperature denitrification pseudomonas fluorescens | |
| CN107699513A (en) | A kind of special degradation bacteria of black and odorous water and its application | |
| CN107364955A (en) | A kind of colonization method for being used to handle the filler of sanitary sewage | |
| CN109370954A (en) | It is a kind of for the composite bacteria agent of sewage treatment, preparation method and application method | |
| CN104496031B (en) | A kind of sewage disposal is held concurrently water plant planting matrix fill preparation method and application | |
| CN103740617A (en) | Composite microbial agent, preparation method thereof and salty sewage treatment method | |
| CN105152325B (en) | A kind of cultural method for being resistant to terramycin anaerobic ammonium oxidation granular sludge culture | |
| CN104386829B (en) | A kind of microbial nutrition synergistic agent being applicable to treatment of dyeing wastewater | |
| CN105524860A (en) | Culture method of fungicide for water purification | |
| CN104843870A (en) | Magnetic carrier immobilized microorganism live bacteria preparation and preparation method and application thereof | |
| CN103373760B (en) | Quick start method for treating ammonia-containing wastewater by A/O process | |
| CN109055252A (en) | A kind of heterotrophic nitrification-aerobic denitrification complex microorganism preparations and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130522 Termination date: 20140417 |