CN114262134A - Method for realizing in-situ sludge reduction by using sludge biological agent - Google Patents
Method for realizing in-situ sludge reduction by using sludge biological agent Download PDFInfo
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Abstract
The invention discloses a method for realizing in-situ sludge reduction by utilizing a sludge biological agent, which comprises the steps of firstly, gradually reducing the addition amount of lysozyme powder, removing half of original sludge at each stage, and enriching and domesticating sludge by adding fresh sludge with the same amount; preparing the domesticated sludge into a sludge biological preparation; and putting the prepared sludge biological agent into the sludge to be treated, and carrying out in-situ remediation on the sludge by adopting the sludge biological agent. The biological agent bacteria source used in the method is from the sludge to be treated, and has strong adaptability to the environment and good treatment effect; the method for realizing sludge reduction by using the biological agent prepared from the domesticated sludge is a relatively efficient and environment-friendly sludge treatment method, has mild action conditions, has no special requirements on equipment, and is easy and efficient to operate.
Description
Technical Field
The invention relates to a sludge treatment technology, in particular to a method for realizing in-situ sludge reduction by using a sludge biological agent
Background
With the increase of economy and the rapid development of urbanization, the domestic water consumption and the industrial water consumption of people are increasing day by day, and the urban sewage treatment plant is developing at a high speed. By 2020, the number of urban sewage treatment plants in China reaches more than 5000, the sewage treatment capacity of cities and counties reaches 2.3 billion cubic meters per day, the urban sewage treatment rate reaches 95%, the county sewage treatment rate also reaches 95%, and the cities on the ground level and above realize the full collection and the full treatment of sewage. In the application of water treatment technology, the activated sludge method is most widely applied, more than 90% of municipal sewage and more than 50% of industrial wastewater are treated by the activated sludge method, the activated sludge is a flocculating constituent with strong adsorption and decomposition capacity, the purpose of treating the sewage and the wastewater is achieved by eating away pollutants and then separating mud from water, but a large amount of residual sludge is generated at the same time. According to statistics, the sludge yield in China is more than 6000 million tons (calculated by water content of 80%) in 2020, and 9000 million tons of sludge yield in China is predicted to break through in 2025. However, the sludge treatment starts late due to the long-term 'heavy water and light mud' and the limited financial capital, the development is slow and unsmooth, and the sludge treatment rate is low.
The components of the sludge are complex and comprise organic matters, pathogenic bacteria, heavy metals and other substances. If the sludge is not properly treated, the inside of the sludge is subjected to anaerobic fermentation to generate odor to pollute the surrounding air, meanwhile, due to the characteristic of high water content, pollutants in the sludge can be flushed into a water body by rainwater, and nitrogen and phosphorus rich in the sludge enter the water body to cause eutrophication and pollute underground water and surface water. Part of heavy metals can enter a food chain through fishes, shrimps and the like and return to a dining table, and are accumulated in a human body to cause human body pathological changes. At present, four main methods for disposing sludge are available: land utilization, landfill treatment, building material utilization and incineration disposal. The method is simple and easy to implement, has high treatment efficiency, needs a large field, and causes secondary damage to the land because toxic substances in the sludge can permeate into the soil. Therefore, the harm of the sludge is more concealed and direct and is difficult to be eliminated. The national institute also clearly puts forward the problem of harmless sludge utilization and resource promotion in the fourteen-five program, so that the sludge disposal problem is urgent.
The sludge reduction means that the yield of the sludge in the system is reduced under the condition that the effluent quality of the system is not influenced. Current research starts from two perspectives, with reductions from the distal and in situ, respectively. The tail end sludge reduction means that the volume and the quality of sludge are reduced by a technical method after a sewage treatment process; the in-situ sludge reduction is basically generated from the source of the system sludge generation. In contrast, the in-situ sludge reduction technology can substantially reduce the sludge amount and can solve the root of the sludge problem to the maximum extent. Currently, the mainstream techniques for the in-situ sludge reduction direction include: uncoupling technology, stealth growth technology, biological enhancement technology and the like. However, the methods have the defects of high process modification cost, high toxicity of part of the coupling agent, difficult degradation and the like, and cause harm to the environment.
Therefore, the method for realizing in-situ sludge reduction by using the sludge biological agent, which has the advantages of strong environmental adaptability, simple operation, environmental protection and obvious effect, is the technical problem to be solved at present.
Disclosure of Invention
In order to solve the above-mentioned defects in the prior art, the present invention aims to provide a method for realizing in-situ sludge reduction by using a sludge biological agent. The method has no pollution to the environment, greatly improves the sludge reduction effect, and has easy and efficient operation method.
The invention is realized by the following technical scheme.
The invention provides a method for realizing in-situ sludge reduction by using a sludge biological agent, which comprises the following steps:
taking fresh sludge of a sewage treatment plant, and mixing the fresh sludge with the mass ratio of (40000): (1-3): (10-40) adding lysozyme powder and a trace element solution I into the sludge, periodically replacing fresh sludge and the lysozyme powder, and sequentially reducing the amount of the added lysozyme powder until no lysozyme powder is added, and obtaining acclimated sludge when the removal rate of SS is measured to be more than 30%;
adding an MWD culture solution into the domesticated sludge according to the mass ratio of 3-5: 1, culturing at constant temperature, replacing the MWD culture solution with a liquid culture solution every certain period, when precipitated sludge appears at the bottom of the reactor, determining that the removal rate of SS in the liquid culture solution is more than 30%, and collecting the precipitated sludge to obtain a biological agent;
adding a sludge biological agent into sludge to be treated, and mechanically stirring; putting biological agents at intervals; regularly discharging mud; and finishing the sludge reduction process.
In the above provided technical solution, the trace element solution I includes:
according to mass concentration, 0.5-1.0g/L EDTA, 0.15-0.20g/L H3BO3、0.15-0.20g/L Na2MoO4·2H2O、0.1-0.3g/L ZnSO4、0.05-0.08g/L MnCl2·4H2O、0.1-0.3g/L FeCl3、0.05-0.10g/L CuSO4·5H2O、0.1-0.3g/L CoCl2·6H2O, pH 7.0 aqueous solution.
In the technical scheme provided above, the MWD culture fluid comprises the following raw materials by mass:
8-10g/L of sodium acetate, 3-5g/L of ammonium chloride, 5-7g/L of dipotassium phosphate, 2mL of trace element solution II, 1000mL of distilled water and a water solution with the pH value of 7.0;
the microelement solution II comprises: 0.2-0.4g/L CoCl2·6H2O，0.4-0.6g/L MnCl2·4H2O，0.1-0.3g/L ZnCl2，0.1-0.3g/L NiCl2·6H2O，0.10-0.20g/L CuSO4·5H2O，0.05-0.10g/L Na2SeO4·2H2O, pH 7.0 aqueous solution.
In the technical scheme provided above, the sludge acclimation in the step 1 includes the following steps:
11) adding lysozyme powder and a trace element solution I into fresh sludge, culturing and domesticating for 2-3d, removing supernatant when the SS removal rate is measured to be more than 30%, and culturing the obtained precipitate again; the culture conditions include: the reaction temperature is kept between 25 and 30 ℃, and the rotating speed is 150 and 180 rpm;
12) and (3) when the SS removal rate is measured to be more than 30% every certain period, removing half of the sludge, adding the same amount of fresh sludge, and sequentially reducing the amount of added lysozyme powder until no lysozyme powder is added, and when the SS removal rate is measured to be more than 30%, obtaining the domesticated sludge.
In the technical scheme provided above, in the step 1, the adding amount of the lysozyme powder is 25mg, 20mg, 15mg, 10mg, 5mg and 0mg in sequence at regular intervals.
In the above provided technical solution, in step 2, the MWD culture fluid is replaced once every certain period, and the replacing of the MWD culture fluid is sequentially performed according to the different volumes of the MWD culture fluid:
a liquid culture medium consisting of MWD culture solution, MWD culture solution with a volume of 3 and sterilized sludge to be treated with a volume of 1;
a liquid culture medium consisting of 2 volumes of MWD culture solution and 1 volume of sterilized sludge to be treated;
1 volume of MWD culture solution and 1 volume of sterilized sludge to be treated.
In the technical scheme provided above, in step 3, 2 operation modes are selected according to the requirements of different sewage plants:
scheme 1: adding the biological agent of the sludge and 1-5% of the volume of the sludge to be treated, mechanically stirring at the rotating speed of 10-50rpm, and adding the biological agent again at intervals; regularly discharging mud;
scheme 2: adding the biological agent of the sludge and 5-10% of the volume of the sludge to be treated, mechanically stirring at the rotating speed of 10-50rpm, and adding the biological agent again at intervals; and (5) periodically discharging the sludge.
In the technical scheme provided above, in scheme 1, the retention time of the biological agent in the reactor is 12-24 h;
and (3) regularly discharging the sludge, and adding the biological agent into the sludge to be treated again after all the sludge is discharged, wherein the interval between two sludge discharges is 12-24 h.
In the technical scheme provided above, in scheme 2, after 5-10% of the biological agent is added for the first time, 1-5% of the biological agent is supplemented every 24-72 hours;
and (3) regularly discharging the sludge, wherein the interval between two times of sludge discharging is 12-24h, and the total sludge amount discharged each time is 20-40%.
Compared with the prior sludge reduction technology, the method has the following beneficial effects:
(1) the sludge biological preparation sludge reduction technology of the invention utilizes original sludge as a bacteria source, obtains a biological preparation through sludge domestication, and reduces sludge through a biological preparation direct-throwing mode. The biological agent is put into the sludge to be treated for 24 hours of continuous and stable operation, the SS removal rate is not lower than 32.12 percent, and the VSS removal rate is not lower than 34.13 percent.
(2) The biological agent bacteria source used in the invention is from the sludge to be treated, and has strong adaptability to the environment and good treatment effect; compared with other treatment methods, the method for reducing the sludge by using the biological agent prepared from the domesticated sludge is efficient and environment-friendly, the action condition is mild, no special requirements are required on equipment, and the operation method is easy and efficient.
(3) According to the invention, less culture solution is added in the sludge enrichment and domestication process, the sludge is enriched by replacing fresh sludge, and the sludge reduction cost is low.
(4) According to the invention, two biological agent adding modes are provided according to the properties of the sludge and the actual requirements of a sewage treatment plant, and the operability is high.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this application, illustrate embodiment(s) of the invention and together with the description serve to explain the principles of the invention:
FIG. 1 is a graph showing the comparative analysis of MLSS and MLVSS removal rates of different biological agents in example 1;
FIG. 2 is a graph showing the change in MLSS removal rate under different dosing amounts of the biological agent in example 1;
FIG. 3 is a graph showing the change in MLVSS removal rate under different bio-agent dosage conditions in example 1;
FIG. 4 is a graph showing the change in MLSS removal rate with the addition of the biological agent in example 2;
FIG. 5 is a graph showing the change in MLVSS removal rate when the biological agent was added in example 2.
Detailed Description
The present invention will now be described in detail with reference to the drawings and specific embodiments, wherein the exemplary embodiments and descriptions of the present invention are provided to explain the present invention without limiting the invention thereto.
The embodiment of the invention provides a method for realizing in-situ sludge reduction by using a sludge biological agent, which comprises the following steps:
taking fresh sludge from a secondary sedimentation tank of a sewage treatment plant into a beaker, wherein the fresh sludge is obtained by mixing the raw materials according to the mass ratio of 40000: (1-3): (10-40) adding lysozyme powder and the trace element solution I into the mixture, uniformly mixing, placing the mixture into a 1000ml conical flask, culturing for 2-3 days at a constant temperature, keeping the reaction temperature at 25-30 ℃, and rotating at the speed of 150-; when the SS removal rate is measured to be more than 30%, removing the supernatant, and culturing the obtained precipitate again; then removing half of the sludge at regular intervals, adding an equal amount of fresh sludge to enable lysozyme producing bacteria to become dominant flora, and sequentially reducing the amount of lysozyme powder, wherein in one embodiment, the amount of the lysozyme powder is sequentially 25mg, 20mg, 15mg, 10mg, 5mg and 0mg until no lysozyme powder is added, and obtaining the acclimated sludge when the SS removal rate is measured to be more than 30%.
Wherein, the microelement solution I comprises:
according to mass concentration, 0.5-1.0g/L EDTA, 0.15-0.20g/L H3BO3、0.15-0.20g/L Na2MoO4·2H2O、0.1-0.3g/L ZnSO4、0.05-0.08g/L MnCl2·4H2O、0.1-0.3g/L FeCl3、0.05-0.10g/L CuSO4·5H2O、0.1-0.3g/L CoCl2·6H2O, pH 7.0 aqueous solution.
collecting the domesticated and enriched sludge, adding an MWD culture solution into the sludge according to the mass ratio of 3-5: 1, culturing for 2-3 days at a constant temperature, pouring off a supernatant, culturing the obtained precipitate again, replacing the MWD culture solution with a liquid culture solution at regular intervals, and sequentially changing the volume of the MWD culture solution according to the difference:
a liquid culture medium consisting of MWD culture solution, MWD culture solution with a volume of 3 and sterilized sludge to be treated with a volume of 1;
a liquid culture medium consisting of 2 volumes of MWD culture solution and 1 volume of sterilized sludge to be treated;
1 volume of MWD culture solution and 1 volume of sterilized sludge to be treated.
When precipitated sludge is formed at the bottom of the reactor, measuring that the removal rate of SS in the liquid culture solution is more than 30%, and collecting the precipitated sludge to obtain the biological agent.
The MWD culture solution comprises the following raw materials in percentage by mass:
8-10g/L of sodium acetate, 3-5g/L of ammonium chloride, 5-7g/L of dipotassium phosphate, 2mL of trace element solution II, 1000mL of distilled water and a water solution with the pH value of 7.0;
the microelement solution II comprises: 0.2-0.4g/L CoCl2·6H2O，0.4-0.6g/L MnCl2·4H2O，0.1-0.3g/L ZnCl2，0.1-0.3g/L NiCl2·6H2O，0.10-0.20g/L CuSO4·5H2O，0.05-0.10g/L Na2SeO4·2H2O, pH 7.0 aqueous solution.
according to the requirements of different sewage plants, the following 2 operation modes can be selected.
Scheme 1: adding a sludge biological agent into sludge to be treated, and carrying out in-situ sludge treatment on the sludge to be treated, wherein the volume of the added sludge biological agent is 1-5% of the volume of the sludge to be treated, and the retention time of the biological agent in a reactor is 12-24 h; the whole process adopts a mechanical stirring mode with the rotating speed of 10-50 rpm; adding biological agents again at intervals; regularly discharging sludge, and putting biological agents into the sludge to be treated again after the sludge is completely discharged, wherein the interval between two sludge discharges is 12-24 hours; and finishing the sludge reduction process.
Scheme 2: adding a sludge biological agent into sludge to be treated, carrying out in-situ sludge treatment on the sludge to be treated, wherein the volume of the sludge biological agent added for the first time is 5-10% of the volume of the sludge to be treated, supplementing 1-5% of the biological agent every 24-72 hours later, and adopting a mechanical stirring mode with the rotating speed of 10-50rpm in the whole process; sludge is discharged regularly, the interval between two sludge discharges is 12-24h, and 20-40% of the total sludge is discharged each time; and finishing the sludge reduction process.
The invention is further illustrated by the following specific examples.
Example 1:
the example provides a method for reducing sludge, and the sludge to be treated in the example is from a secondary sedimentation tank of Shanxi drum Water Co Ltd in Tong district of Xian city. According to the technical scheme of the invention, the biological treatment of the sludge to be treated comprises the following steps:
In the first stage, fresh sludge is taken and put into a beaker, and the mass ratio of the fresh sludge to the fresh sludge is 40000: 1: 40 adding 25mg lysozyme powder and 1ml trace element solution I, mixing uniformly, placing in a 1000ml conical flask, sealing the conical flask mouth with a sealing film, culturing and acclimatizing for 2d at 30 deg.C and 180rpm, when the SS removal rate is 34.83%, entering a second stage, removing half of the original sludge, adding fresh sludge with the same volume, adding 20mg lysozyme powder and 2ml trace elements, culturing and acclimatizing at the same temperature and rotation speed, after 2d, when the SS removal rate is 30.23%, entering a third stage, similarly removing 1/2 sludge, adding fresh sludge with the same volume, adding 15mg lysozyme powder and 2ml trace elements, culturing and acclimatizing, after 2d, when the SS removal rate is 31.33%, entering a fourth stage, removing half of original sludge, adding fresh sludge with the same volume, adding 10mg of lysozyme powder and 2ml of trace elements, culturing and acclimating at 30 ℃ and 180rpm, after culturing and acclimating for 2d, when the removal rate of SS is measured to be 33.52%, then entering a fifth stage, similarly removing 1/2 of sludge, adding fresh sludge with the same volume, adding 5mg of lysozyme powder and 2ml of trace elements, culturing and acclimating, after acclimating for 2d, when the removal rate of SS is measured to be 33.25%, entering a sixth stage, removing half of original sludge, adding fresh sludge with the same volume, adding only 2ml of trace elements for culturing and acclimating without adding lysozyme powder, and after acclimating for 2d, when the removal rate of SS is measured to be 32.37%, acclimating is completed.
The trace element solution I is: by mass concentration, 0.5g/L EDTA, 0.20g/L H3BO3、0.15g/L Na2MoO4·2H2O、0.3g/L ZnSO4、0.08g/L MnCl2·4H2O、0.1g/L FeCl3、0.10g/L CuSO4·5H2O、0.3g/L CoCl2·6H2O, pH 7.0 aqueous solution.
collecting the domesticated and enriched sludge, adding MWD culture solution into the sludge according to the mass ratio of 3:1, and putting the sludge into a constant-temperature incubator for culture. During the whole experiment, the temperature of the reactor is kept at 30 ℃ under aerobic conditions, and the rotating speed is 160 rpm. Culturing for 2 days, pouring out the supernatant, culturing the obtained precipitate again, replacing the MWD culture solution with the liquid culture solution at regular intervals, measuring the SS removal rate in the liquid culture solution to be more than 30% when precipitated sludge is formed at the bottom of the reactor, and collecting the precipitated sludge to obtain the biological microbial inoculum.
Replacing the liquid culture solution once every certain period, wherein the replacement sequence is as follows: the method comprises the following steps of (1) preparing MWD culture solution, 3 volumes of MWD culture solution, 1 volume of liquid culture medium consisting of sterilized sludge to be treated, 2 volumes of MWD culture solution, 1 volume of liquid culture medium consisting of sterilized sludge to be treated, 1 volume of MWD culture solution, 1 volume of liquid culture medium consisting of sterilized sludge to be treated, and sterilized sludge to be treated;
the MWD culture solution comprises the following raw materials in percentage by mass:
8g/L of sodium acetate, 5g/L of ammonium chloride, 5g/L of dipotassium phosphate, 2mL of trace element solution II, 1000mL of distilled water and an aqueous solution with the pH value of 7.0.
The microelement solution II comprises: in terms of mass concentration, 0.3g/L CoCl2·6H2O，0.5g/L MnCl2·4H2O，0.1g/L ZnCl2，0.1g/L NiCl2·6H2O，0.20g/L CuSO4·5H2O，0.05g/L Na2SeO4·2H2O, pH 7.0 aqueous solution.
As can be seen from fig. 1, 2 and 3, when the addition amount of the biological agent in the control group is 0%, the removal rate of SS is 7.23% and the removal rate of VSS is 7.89% under the metabolic action of the original microorganisms in the sludge, and the removal rates of SS and VSS after the addition of the biological agent in the sludge are both more than 28%. The results show that the addition of the biological agent effectively reduces the sludge. And it can be found that the quantity of lysozyme producing bacteria is rapidly increased and the removal rate of SS and VSS is rapidly improved within the time range of 3-12h due to the addition of the biological microbial inoculum, the increase of the removal rate of SS and VSS is slowed down, the removal rate of SS and VSS is gradually stabilized after 12h, and finally the removal rate of SS is up to 32.92 percent and 25.69 percent higher than that of a control group, and the removal rate of VSS is up to 34.13 percent and 26.24 percent higher than that of the control group after 24h of continuous stable operation. Therefore, the addition of the sludge biological agent can obviously improve the reduction rate of the sludge.
Example 2:
the example provides a method for reducing sludge, and the sludge to be treated in the example is from a secondary sedimentation tank of a sewage treatment plant of the university of the Western's engineering. According to the technical scheme of the invention, the biological treatment of the sludge to be treated comprises the following steps:
In the first stage, fresh sludge is taken and put into a beaker, and the mass ratio of the fresh sludge to the fresh sludge is 40000: 3: 10 adding 25mg lysozyme powder and 0.5ml trace elements, mixing uniformly, placing in a 1000ml conical flask, sealing the conical flask mouth with a sealing film, culturing and acclimatizing in a shaker at 25 ℃, 150rpm for 3d, removing half of original sludge when the removal rate of SS is 35.68%, adding fresh sludge with the same volume, adding 20mg lysozyme powder and 2ml trace elements, culturing and acclimatizing at the same temperature and rotating speed, culturing and acclimatizing for 3d, removing 1/2 sludge when the removal rate of SS is 31.49%, adding fresh sludge with the same volume, adding 15mg lysozyme powder and 2ml trace elements, culturing and acclimatizing, after 3d, when the removal rate of SS is 32.15%, entering a fourth stage, removing half of original sludge, adding fresh sludge with the same volume, adding 10mg of lysozyme powder and 2ml of trace elements, culturing and acclimating at 30 ℃ and 180rpm, after culturing and acclimating for 3d, when the removal rate of SS is measured to be 32.89%, then entering a fifth stage, similarly removing 1/2 sludge, adding fresh sludge with the same volume, adding 5mg of lysozyme powder and 2ml of trace elements, culturing and acclimating, after acclimating for 3d, when the removal rate of SS is measured to be 33.67%, entering a sixth stage, removing half of original sludge, adding fresh sludge with the same volume, adding only 2ml of trace elements for culturing and acclimating, and after acclimating for 3d, when the removal rate of SS is measured to be 33.46%, acclimating is completed.
The trace element solution is as follows: by mass concentration, 1.0g/L EDTA, 0.15g/L H3BO3、0.20g/L Na2MoO4·2H2O、0.1g/L ZnSO4、0.05g/L MnCl2·4H2O、0.1g/L FeCl3、0.05g/L CuSO4·5H2O、0.1g/L CoCl2·6H2O, pH 7.0 aqueous solution.
collecting the domesticated and enriched sludge, adding MWD culture solution into the sludge according to the mass ratio of 5:1, and putting the sludge into a constant-temperature incubator for culture. During the whole experiment, the temperature of the reactor is kept at 30 ℃ under aerobic conditions, and the rotating speed is 180 rpm. Culturing for 3 days, pouring out supernatant, culturing the obtained precipitate again, replacing MWD culture solution with liquid culture solution at regular intervals, measuring SS removal rate in the liquid culture solution to be more than 30% when precipitated sludge is formed at the bottom of the reactor, and collecting the precipitated sludge to obtain the biological preparation.
Replacing the liquid culture solution once every certain period, wherein the replacement sequence is as follows: the method comprises the following steps of (1) preparing MWD culture solution, 3 volumes of MWD culture solution, 1 volume of liquid culture medium consisting of sterilized sludge to be treated, 2 volumes of MWD culture solution, 1 volume of liquid culture medium consisting of sterilized sludge to be treated, 1 volume of MWD culture solution, 1 volume of liquid culture medium consisting of sterilized sludge to be treated, and sterilized sludge to be treated;
the MWD culture solution comprises the following raw materials in percentage by mass:
10g/L of sodium acetate, 3g/L of ammonium chloride, 7g/L of dipotassium phosphate, 2mL of trace element solution II, 1000mL of distilled water and an aqueous solution with the pH value of 7.0.
The microelement solution II comprises: in terms of mass concentration, 0.4g/L CoCl2·6H2O，0.6g/L MnCl2·4H2O，0.3g/L ZnCl2，0.3g/L NiCl2·6H2O，0.10g/L CuSO4·5H2O，0.10g/L Na2SeO4·2H2O, water with pH 7.0And (3) solution.
As can be seen from the graphs in FIGS. 4 and 5, the sludge reduction rate of the sludge biological agent can be obviously improved through continuous measurement for 24 hours, the quantity of lysozyme-producing bacteria is rapidly increased due to the addition of the biological microbial agent between 0 and 3 hours, the SS removal rate is rapidly increased to 23.13 percent, the VSS removal rate is increased to 25.25 percent, the increase potential of the SS removal rate and the VSS removal rate is slowed down within the time range of 3 to 12 hours, after 12 hours, the SS removal rate and the VSS removal rate are gradually stabilized, and after continuous stable operation for 24 hours, the SS removal rate is finally 32.12 percent, is higher than that of a control group 25.27 percent, the VSS removal rate is 34.53 percent, and is higher than that of the control group 26.79 percent. Therefore, the addition of the sludge biological agent can obviously improve the reduction rate of the sludge.
The present invention is not limited to the above-mentioned embodiments, and based on the technical solutions disclosed in the present invention, those skilled in the art can make some substitutions and modifications to some technical features without creative efforts according to the disclosed technical contents, and these substitutions and modifications are all within the protection scope of the present invention.
Claims (10)
1. A method for realizing in-situ sludge reduction by using a sludge biological agent is characterized by comprising the following steps:
step 1, sludge domestication:
taking fresh sludge of a sewage treatment plant, and mixing the fresh sludge with the mass ratio of 40000: (1-3): (10-40) adding lysozyme powder and a trace element solution I into the sludge, periodically replacing fresh sludge and the lysozyme powder, and sequentially reducing the amount of the added lysozyme powder until no lysozyme powder is added, and obtaining acclimated sludge when the removal rate of SS is measured to be more than 30%;
step 2, preparing a biological agent:
adding an MWD culture solution into the domesticated sludge according to the mass ratio of 3-5: 1, culturing at constant temperature, replacing the MWD culture solution with a liquid culture solution every certain period, when precipitated sludge appears at the bottom of the reactor, determining that the removal rate of SS in the liquid culture solution is more than 30%, and collecting the precipitated sludge to obtain a biological agent;
step 3, the operation mode is as follows:
adding a sludge biological agent into sludge to be treated, and mechanically stirring; putting biological agents at intervals; regularly discharging mud; and finishing the sludge reduction process.
2. The method according to claim 1, wherein the trace element solution I comprises:
according to mass concentration, 0.5-1.0g/L EDTA, 0.15-0.20g/L H3BO3、0.15-0.20g/LNa2MoO4·2H2O、0.1-0.3g/L ZnSO4、0.05-0.08g/L MnCl2·4H2O、0.1-0.3g/L FeCl3、0.05-0.10g/L CuSO4·5H2O、0.1-0.3g/L CoCl2·6H2O, pH 7.0 aqueous solution.
3. The method of claim 1, wherein the MWD culture fluid comprises the following raw materials in mass ratio:
8-10g/L of sodium acetate, 3-5g/L of ammonium chloride, 5-7g/L of dipotassium phosphate, 2mL of trace element solution II, 1000mL of distilled water and a water solution with the pH value of 7.0;
the microelement solution II comprises: 0.2-0.4g/L CoCl2·6H2O，0.4-0.6g/L MnCl2·4H2O，0.1-0.3g/L ZnCl2，0.1-0.3g/L NiCl2·6H2O，0.10-0.20g/L CuSO4·5H2O，0.05-0.10g/LNa2SeO4·2H2O, pH 7.0 aqueous solution.
4. The method according to claim 1, wherein the sludge acclimatization in the step 1 comprises the following steps:
11) adding lysozyme powder and a trace element solution I into fresh sludge, culturing and domesticating for 2-3d, removing supernatant when the SS removal rate is measured to be more than 30%, and culturing the obtained precipitate again; the culture conditions include: the reaction temperature is kept between 25 and 30 ℃, and the rotating speed is 150 and 180 rpm;
12) and (3) when the SS removal rate is measured to be more than 30% every certain period, removing half of the sludge, adding the same amount of fresh sludge, and sequentially reducing the amount of added lysozyme powder until no lysozyme powder is added, and when the SS removal rate is measured to be more than 30%, obtaining the domesticated sludge.
5. The method according to claim 4, wherein in step 1, the lysozyme powder is added in an amount of 25mg, 20mg, 15mg, 10mg, 5mg and 0mg in sequence at regular intervals.
6. The method of claim 1, wherein in step 2, the MWD culture solution is replaced at regular intervals, and the MWD culture solution replacement volume is different in the following order:
a liquid culture medium consisting of MWD culture solution, MWD culture solution with a volume of 3 and sterilized sludge to be treated with a volume of 1;
a liquid culture medium consisting of 2 volumes of MWD culture solution and 1 volume of sterilized sludge to be treated;
1 volume of MWD culture solution and 1 volume of sterilized sludge to be treated.
7. The method of claim 1, wherein in the step 3, 2 operation modes are selected according to the requirements of different sewage plants:
scheme 1: adding the biological agent of the sludge and 1-5% of the volume of the sludge to be treated, mechanically stirring at the rotating speed of 10-50rpm, and adding the biological agent again at intervals; regularly discharging mud;
scheme 2: adding the biological agent of the sludge and 5-10% of the volume of the sludge to be treated, mechanically stirring at the rotating speed of 10-50rpm, and adding the biological agent again at intervals; and (5) periodically discharging the sludge.
8. The method of claim 7, wherein in scheme 1, the residence time of the biological agent in the reactor is 12-24 h;
and (3) regularly discharging the sludge, and adding the biological agent into the sludge to be treated again after all the sludge is discharged, wherein the interval between two sludge discharges is 12-24 h.
9. The method according to claim 7, wherein in case 2, 1-5% of the biological agent is supplemented every 24-72 hours after 5-10% of the biological agent is added for the first time;
and (3) regularly discharging the sludge, wherein the interval between two times of sludge discharging is 12-24h, and the total sludge amount discharged each time is 20-40%.
10. The method as claimed in claim 7, wherein the continuous stable operation of 24 hours of adding the biological agent into the sludge to be treated has SS removal rate not lower than 32.12% and VSS removal rate not lower than 34.13%.
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