CN102531197A - Bioactive multilayer covering remediation method for contaminated sediment - Google Patents

Bioactive multilayer covering remediation method for contaminated sediment Download PDF

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CN102531197A
CN102531197A CN2011104504704A CN201110450470A CN102531197A CN 102531197 A CN102531197 A CN 102531197A CN 2011104504704 A CN2011104504704 A CN 2011104504704A CN 201110450470 A CN201110450470 A CN 201110450470A CN 102531197 A CN102531197 A CN 102531197A
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water
biozeolite
liquid
river sand
covers
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CN102531197B (en
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黄廷林
周真明
苏俊峰
王改
董玉华
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Xian University of Architecture and Technology
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Xian University of Architecture and Technology
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Abstract

The invention discloses a bioactive multilayer covering remediation method for contaminated sediments. Bioactive multilayer covering with a synergistic effect of river sand and natural zeolite for fixing high-efficiency nitrifying bacteria and denitrifying bacteria is adopted for reducing the amount of contaminants released towards a water body in the sediments and controlling the eutrophication of the water body. The method comprises the steps of: using the river sand and the biozeolite as covering materials, inoculating highly-effective strain bacteria solution obtained through screening into liquid culture medium, adding enriched nitrifying bacteria and denitrifying bacteria liquid into sterilized eutrophic raw water, pouring turbid raw water into a container containing a certain amount of zeolite after the raw water becomes turbid, forming a biofilm, controlling the concentration of dissolved oxygen (DO) to be 2-3mg/L through an aeration device to accelerate biofilm formation, and after three days of continuous aeration for biofilm formation, using continuous water to wash away bacteria which are adsorbed on the surfaces of fillers. The prepared biozeolite and the clean river sand evenly cover the sediments in a multilayer covering way of laying the biozeolite at a lower layer and the river sand at an upper layer. Therefore, a bioactive multilayer covering technique is used for realizing the in-situ remediation of the contaminated sediments.

Description

Pollution deposit microbic activity multilayer covers restorative procedure
Technical field
The invention belongs to water-treatment technology field, be specifically related to a kind of biological activity multilayer that adopts and cover the technology of repairing Marine sediments, be specially adapted to endogenous nitrogen, the serious sedimental reparation of river, lake and reservoir of phosphorus load.
Background technology
Body eutrophication is the difficult problem that current water pollution is administered, and has become one of global most important environmental problem.Settling is N in the water body, P " source " or " remittance ", and N, P concentration are the main limiting factor of body eutrophication.Under the situation that external source N, P are effectively controlled, the release of the endogenous N of control settling, P will become the effective measure of control body eutrophication.The method of cutting down the endogenous release of Marine sediments at present has methods such as nature decay, dredging and covering.The nature damped method is applicable to that source of pollution are effectively controlled, and deposition and diluting effect environment faster.The dredging method mainly have problems the settling resuspending to water body pollute, settling transportation and treating processes causes secondary pollution, expense is high and to the destruction of water body biology community structure etc.Cladding process is world today's research focus; Physics masking layer by traditional thick develops into current thin active tectum; The active cladding process existing problems of thin layer have: active covering material will be lost ability to function to pollutent absorption after saturated, and its long-term effectiveness has much room for improvement; Inhomogeneous covering makes the part settling not be capped in the practical application, causes the sediment pollution thing to be directly released into water body and the release of bottom-dwelling disturbance promotion sediment pollution thing.
Summary of the invention
There is defective or not enough to prior art; The objective of the invention is to; Provide a kind of pollution deposit microbic activity multilayer to cover restorative procedure, can effectively cut down the burst size of pollutents such as nitrogen in the Marine sediments, phosphorus and organism, strengthen the tectum long-term effectiveness to water body; Reduce running cost, the control body eutrophication.
In order to realize above-mentioned technical assignment, the present invention adopts following technical scheme to be achieved:
1) select using natural clinoptilolite and river sand as the Marine sediments covering material;
2) high-efficiency strain enrichment: screening is obtained nitrobacteria and denitrifying bacterium is inoculated in respectively in nitrobacteria liquid nutrient medium and the denitrifying bacterium liquid nutrient medium; 2-3d is cultivated in vibration under 25-30 ℃, 120rpm constant temperature, obtains enrichment nitrobacteria and denitrifying bacterium bacterium liquid respectively.
The prescription of described nitrobacteria substratum is: sodium acetate 0.3gL -1, potassium hydrogenphosphate 0.02gL -1, magnesium chloride 0.05gL -1, ammonium chloride 0.06gL -1, liquid microelement 2mL; Wherein micro-liquid formula is: with EDTA 50g, ZnSO 42.2g, CaCl 25.5g, MnCl 24H 2O 5.06g, FeSO 47H 2O 5.0g, ammonium molybdate 1.1g, CuSO 45H 2O 1.57g, CoCl 26H 2O 1.61g joins in the 1000mL deionized water, uses 1molL again -1HCl and 1molL -1NaOH regulates pH=7;
The prescription of described denitrifying bacterium substratum is: sodium acetate 0.3gL -1, potassium hydrogenphosphate 0.02gL -1, magnesium chloride 0.05gL -1, SODIUMNITRATE 0.06gL -1, liquid microelement 2mL, wherein liquid microelement is formulated as: with EDTA 50g, ZnSO 42.2g, CaCl 25.5g, MnCl 24H 2O 5.06g, FeSO 47H 2O 5.0g, ammonium molybdate 1.1g, CuSO 45H 2O1.57g, CoCl 26H 2O 1.61g joins in the 1000mL deionized water, uses 1molL again -1HCl and 1molL -1NaOH regulates pH=7;
3) zeolite microorganism colonization: at first former water is sterilized in High pressure steam sterilizer, sterilising conditions is 120～126 ℃ of temperature, pressure 0.10～0.14MPa; Time 30min, nitrobacteria that then enrichment is obtained and the bacterium liquid of denitrifying bacterium are to carry out proportioning at 1: 1 to obtain mixed bacteria liquid according to volume ratio, are to add to former water at 1: 9 mixed bacteria liquid and former water according to volume ratio then; Constant temperature water bath vibration 2～3d under 25-30 ℃, 120rpm condition; After former water becomes muddiness, the former water of muddiness is poured in the device that fills the zeolite appearance, muddy former water liquid level exceeds zeolite 5cm and gets final product; Hanging film is controlled at the concentration of DO through aerating apparatus and quickens to hang the film process more than the 2-3mg/L; After continuous aeration is hung film 3d, adopt solid stream of water to wash out the bacterium of the absorption of filling surface, obtain biozeolite;
4) covering material adds: at last multilayer coverage mode covering material is evenly covered and carry out biological prosthetic on the settling at following, river sand according to biozeolite.
Other technologies characteristics of the present invention are: described using natural clinoptilolite particle diameter is 1～2mm, and covering intensity is 2～3kg/m 2, cladding thickness is 2～3mm, and the particle diameter of river sand is 0.15～0.20mm, and covering intensity is 10kg/m 2, cladding thickness is 1.1cm.
Pollution deposit microbic activity multilayer of the present invention covers restorative procedure, can effectively cut down the burst size of pollutents such as nitrogen in the Marine sediments, phosphorus and organism to water body, strengthens the tectum long-term effectiveness, reduces running cost, the control body eutrophication.The present invention compared with prior art has following technological merit: the burst size of pollutents such as nitrogen in the settling, phosphorus and organism to water body effectively cut down in (1), simultaneously Fe, Mn also had certain inhibition effect; (2) bio-regeneration of zeolite strengthens the tectum long-term effectiveness; (3) reduce biozeolite and cover intensity, reduce technical costs; (4) control body eutrophication.
Description of drawings
Fig. 1-Fig. 4 is that experimental verification biozeolite biozeolite of the present invention and river sand various combination mode cover reparation Marine sediments effect experimental result picture, and wherein Fig. 1 is TN concentration change figure in the different system overlying water, and Fig. 2 is NH in the different system overlying water 4 +-N concentration change figure, Fig. 3 are NO in the different system overlying water 2 --N concentration change figure, Fig. 4 are NO in the different system overlying water 3 --N concentration change figure.
Fig. 5-Fig. 8 covers reduction overlying water nitrogen load effect experiment effect figure for multilayer of the present invention covers with individual layer, and wherein Fig. 5 is the variation diagram of TN concentration in the different system overlying water, and Fig. 6 is NH in the different system overlying water 4 +The variation diagram of-N concentration; Fig. 7 is NO in the different system overlying water 2 -The variation diagram of-N concentration, Fig. 8 are NO in the different system overlying water 3 --N concentration change figure.
Fig. 9-Figure 12 covers intensity river sand and biozeolite multilayer and covers and cut down the overlying water nitrogen design sketch of loading for the present invention is different, the variation diagram of TN concentration in Fig. 9 different system overlying water wherein, and Figure 10 is NH in the different system overlying water 4 +The variation diagram of-N concentration; Figure 11 is NO in the different system overlying water 2 -The variation diagram of-N concentration, Figure 12 are NO in the different system overlying water 3 --N concentration change figure.
Below in conjunction with accompanying drawing particular content of the present invention is done further explain.
Embodiment
The high-efficiency strain that the present invention relates to screen obtains nitrobacteria SY1 (pseudomonas SY1) and denitrifying bacterium HY1 (pseudomonas HY1) for screening from the reservoir bed mud of Heihe; This two bacterial classification is preserved in Chinese typical culture collection center on August 24th, 2009; Be called for short CCTCC; And register on the books, this biological inoculum was preserved 30 years on August 24th, 2009.Nitrobacteria pseudomonas SY1 (Pseudomonas sp.SY1) wherein, preserving number is: CCTCCNO:M 209181; Denitrifying bacterium pseudomonas HY1 (Pseudomonas sp.HY1) preserving number is: CCTCCNO:M 209180.
Biological activity multilayer of the present invention covers the Marine sediments technology of repairing; Its adopts covering material is the i.e. fixing natural zeolite of high efficiency nitrification bacterium and denitrifying bacterium (being called biozeolite) of river sand and biological activity covering material; Coverage mode is that biozeolite is descending, and river sand is last.
Pollution deposit microbic activity multilayer of the present invention covers restorative procedure, and this method comprises the following steps:
1) selects using natural clinoptilolite and river sand as the Marine sediments covering material, wherein select for use using natural clinoptilolite as microbial fixed carrier.Select using natural clinoptilolite for use, its advantage has specific surface area big, porous; Easy to be filmed can be created the anaerobism of suitable microorganism growth, facultative and aerobic varying environment, and ammonium ion is had good exchange capacity; But ammonium adsorptive power bio-regeneration, physical and chemical performance is stable, nontoxicity; Hydraulics is abundant, and is cheap, and the place of production is abundant.The using natural clinoptilolite particle diameter that the present invention selects for use is 1～2mm, and covering intensity is 2～3kg/m 2, cladding thickness is 2～3mm.
Select for use and use river sand, its advantage physical and chemical performance is stable, nontoxicity, and it is strong, cheap to shelter performance, and the source of goods is wide.The particle diameter of the river sand that the present invention selects for use is 0.15～0.20mm, and covering intensity is 10kg/m 2, cladding thickness is 1.1cm.
2) high-efficiency strain enrichment: screening is obtained nitrobacteria and denitrifying bacterium is inoculated in respectively in nitrobacteria liquid nutrient medium and the denitrifying bacterium liquid nutrient medium; 2-3d is cultivated in vibration under 25-30 ℃, 120rpm constant temperature, obtains enrichment nitrobacteria and denitrifying bacterium bacterium liquid respectively.
Wherein screening obtains the nitrobacteria denitrifying bacterium and is from the reservoir bed mud of Heihe screening to obtain nitrobacteria be pseudomonas SY1 (Pseudomonas sp.SY1); Deposit number CCTCCNO:M 209181 is pseudomonas HY1 (Pseudomonas sp.HY1) with denitrifying bacterium; Deposit number CCTCCNO:M 209180 is inoculated in respectively in nitrobacteria liquid nutrient medium and the denitrifying bacterium liquid nutrient medium, 25-30 ℃, 120rpm constant-temperature shaking cultivation 2-3d;
Wherein nitrobacteria SY1 and denitrifying bacterium HY1 bacterial strain all belong to Rhodopseudomonas, and it has following characteristic separately:
(a) colony morphology characteristic and morphological features are referring to table 2.
Figure BDA0000122574740000041
Table 2
(b) major physiological, biochemical character, referring to table 3:
Figure BDA0000122574740000042
Table 3
(annotate: √ expresses support for, and+expression is grown or reacted positive)
(c) NH 4 +-N, NO 3 ---the N clearance
The ammonia nitrogen starting point concentration is 1.26mg/L, is 0.0112mg/L through ammonia nitrogen ultimate density after the cultivation of 48h, is 99.11% to ammonia nitrogen removal frank.
The nitrate salt starting point concentration is 2.3mg/L, is 0.041mg/L through nitrate salt ultimate density after the cultivation of 48h, is 98.52% to the nitrate removal rate.Referring to table 4:
But bacterial strain Starting point concentration (mg/L) Ultimate density (mg/L) Clearance (%)
SY1 1.26 0.0112 99.11
HY1 2.3 0.041 98.52
Table 4
Wherein the prescription of nitrobacteria pseudomonas SY1 substratum is: sodium acetate 0.3gL -1, potassium hydrogenphosphate 0.02gL -1, magnesium chloride 0.05gL -1, ammonium chloride 0.06gL -1, liquid microelement 2mL; Wherein micro-liquid formula is: EDTA 50g, ZnSO 42.2g, CaCl 25.5g, MnCl 24H 2O 5.06g, FeSO 47H 2O 5.0g, ammonium molybdate 1.1g, CuSO 45H 2O1.57g, CoCl 26H 2O 1.61g joins in the 1000mL deionized water, uses 1molL again -1HCl and 1molL -1NaOH regulates pH=7;
The prescription of denitrifying bacterium pseudomonas HY1 substratum is: sodium acetate 0.3gL -1, potassium hydrogenphosphate 0.02gL -1, magnesium chloride 0.05gL -1, SODIUMNITRATE 0.06gL -1, liquid microelement 2mL, wherein liquid microelement is formulated as: EDTA 50g, ZnSO 42.2g, CaCl 25.5g, MnCl 24H 2O 5.06g, FeSO 47H 2O 5.0g, ammonium molybdate 1.1g, CuSO 45H 2O1.57g, CoCl 26H 2O 1.61g joins in the 1000mL deionized water, uses 1molL again -1HCl and 1molL -1NaOH regulates pH=7.
3) zeolite microorganism colonization: at first former water is sterilized in High pressure steam sterilizer; Sterilising conditions is 120～126 ℃ of temperature; Pressure 0.10～0.14MPa, time 30min, nitrobacteria that then enrichment is obtained and the bacterium liquid of denitrifying bacterium are to carry out proportioning at 1: 1 to obtain mixed bacteria liquid according to volume ratio; Be to add to former water at 1: 9 mixed bacteria liquid and former water according to volume ratio then, constant temperature water bath vibration 2～3d under 25-30 ℃, 120rpm condition.After former water becomes muddiness; The former water of muddiness is poured in the device that fills the zeolite appearance; Muddy former water liquid level exceeds zeolite 5cm and get final product, hangs film and through aerating apparatus the concentration of DO is controlled at and quickens to hang the film process more than the 2-3mg/L, behind the continuous aeration extension film 3d; Adopt solid stream of water to wash out the bacterium of the absorption of filling surface, obtain biozeolite.
4) covering material adds: according to biozeolite down, river sand is at last multilayer coverage mode, and covering material is evenly covered on the settling, realizes that Marine sediments are carried out the biological activity multilayer to be covered and repair.
Experimental verification:
Abide by above-mentioned steps of the present invention and technical essential, the present invention is a research object with ancient former water in canal in the Yangzhou of eutrophication and surface deposit, has carried out indoor little, the middle experimental study in laboratory respectively.
One, the best multilayer coverage mode of biozeolite and river sand is confirmed
(1) testing program
Test is at DN200,, carry out in the 10L wide-mouth vial; The overlying water depth of water is 15cm (volume is 4.17L), and thickness of deposits is 10cm (quality is about 4912g).Biozeolite and river sand array mode have: combination one, biozeolite are last, and river sand is following; Combination two, biozeolite is following, and river sand is last; According to the river sand particle diameter river sand is divided into rough sand (0.2～2mm) and fine sand (0.15～0.2mm).
Test is provided with 6 vials altogether, is respectively blank, biozeolite and covers that (covering intensity is 5kgm -2, thickness is 5mm), rough sand combination one covers that (it is 23kgm that rough sand covers intensity -2, thickness is 2cm; It is 3kgm that biozeolite covers intensity -2, thickness is 3mm), rough sand combination two covers (parameter is unified on the same group), fine sand combination one and covers that (it is 18.6kgm that fine sand covers intensity -2, thickness is 2cm; It is 3kgm that biozeolite covers intensity -2, thickness is 3mm) and fine sand combination two coverings (parameter is unified on the same group).
Relatively biozeolite and river sand various combination mode cover and repair the Marine sediments effect, confirm best array mode, propose biological activity multilayer soverlay technique concrete grammar.
(2) test-results and analysis
1. cut down overlying water nitrogen load effect
In the process of the test in the overlying water water temperature be that 16～20 ℃, pH7～8, DO are 2.5～6mgL -1(under the natural condition), test lasts 121d; TN, NH in each system's overlying water 4 +-N, NO 2 --N, NO 3 -The variation of-N concentration is respectively like Fig. 1, Fig. 2, Fig. 3, shown in Figure 4.
Can know that by Fig. 1 test lasts 121d, compare that the reduction rate that rough sand combination one, rough sand combination two, fine sand combination one and fine sand combination two cover TN in the overlying water is respectively 17.59%, 33.91%, 30.93% and 52.32% with blank; Combination two covers TN reduction rate has been improved 16.32%～21.39% than combination one; The fine sand combination covers TN reduction rate has been improved 13.34%～18.41% than rough sand; Fine sand combination two covers and covers quite to TN reduction rate with biozeolite, explain that under to TN reduction rate on an equal basis condition, fine sand combination two coverings can significantly reduce the biozeolite overlay capacity, have reduced technical costs.ANOVA showed significant; Each covering system is cut down with blank TN in the overlying water has notable difference (P＜0.05); Biozeolite is subdued with combination two, rough sand combination two and two couples of TN of fine sand combination all has notable difference (P＜0.05), and biozeolite is subdued equal no significant difference (P＞0.05) with combination one, rough sand combination one and fine sand combination one, two couples of TN of combination one and combination.
Can know 24d before the test, NH in the blank system settling by Fig. 2 4 +-N continues to discharge, and on average discharging flux is 102.25mgm -2D -1, NH in each covering system 4 +The average burst size of-N is negative value, and (except rough sand combination two coverings, its value is 0.27mgm -2D -1), explain that the covering of biological activity multilayer has suppressed NH in the settling fully 4 +-N discharges.
Visible by Fig. 3, compare NO in each covering system with blank 2 --N has shortened nearly 60d integration time, explains that the nitrification of biozeolite is obvious, in advance with NO in the water body 2 --N is converted into NO 3 --N.
Visible by Fig. 4, test lasts 121d, compares with blank, and rough sand combination one, rough sand combination two, fine sand combination one and fine sand combination two cover NO in the overlying water 3 -The reduction rate of-N is respectively 21.35%, 73.98%, 24.59% and 73.30%; Combination two covers NO 3 --N reduction rate has improved 48.71%～52.63% than combination one; The fine sand combination covers with the rough sand combination and covers NO 3 --N reduction rate is suitable.
In sum, best multilayer coverage mode is that biozeolite is descending thin river sand last.
2. the biological activity multilayer covers and repairs polluted by nitrogen settling Analysis on Mechanism
Visible by Fig. 1, TN concentration begins to rise gradually in the blank, and 34d reaches peak value, is reduced to certain certain value subsequently gradually and tends towards stability, and explains that ancient canal, Yangzhou water body has certain self-purification capacity.
Combination one of biozeolite, rough sand and fine sand make up in the covering system that 10d descends rapidly before the TN concentration; Tend towards stability subsequently, behind 24d, TN concentration ascendant trend; Behind 34d; TN concentration has slow rising and tends towards stability in the biozeolite covering system, but thick, fine sand makes up TN fast rise in the covering system, tends towards stability behind the 60d; Rough sand combination two and fine sand make up in two covering systems TN concentration to begin 3d and slightly descends, and tend towards stability subsequently, but fine sand makes up two covering systems behind 30d, and TN concentration has slowly downtrending gradually, and back trend is stable again.
Analyze its reason, river sand makes up in the covering system, and initial stage lower floor's river sand covers temporary transient physics and shelters the release of settling nitrogen pollutant; The upper strata biozeolite adsorbs ammonia nitrogen in the overlying water fast; Make that TN concentration descends fast in the water body, but behind the 34d, high concentration N pollutes and has penetrated river sand tectum (this also is the maximum hidden danger of independent husky soverlay technique); Biozeolite less than sand bed polluted by nitrogen releasability, makes TN concentration fast rise in the water body to the adsorptive power of nitrogen.
And river sand makes up in two covering systems; Initial stage upper strata river sand tectum absorption overlying water small amount of nitrogen pollutent; Making that TN slightly reduces again in the overlying water tends towards stability; Lower floor's biozeolite tectum adsorbs settling liberating nitrogen pollutent fast; Husky tectum physical barriers has strengthened polluted by nitrogen concentration, and (test lasts 121d, and rough sand combination one, rough sand combination two, fine sand combination and fine sand combination two system mesolite ammonia nitrogen absorption amount are respectively 1.24,2.99,1.16 and 3.14mgg to have improved biozeolite adsorption of nitrogen pollutent ability -1); And upper strata river sand layer prolongs the duration of contact of pollutent and biozeolite, has strengthened physics, chemistry, the biological action of biozeolite, reduces husky tectum polluted by nitrogen concentration; When N concentration is higher than husky tectum in the overlying water; Overlying water N pollutent moves to sand bed, removed by biozeolite physical and chemical adsorption and biological denitrification gradually, thereby TN concentration has downtrending gradually in the overlying water.
It is thus clear that the biological activity multilayer covers the mechanism of action of repairing Marine sediments: repair Marine sediments through lower floor's biozeolite and upper strata river sand synergy; The river sand layer mainly acts on: (1) physics is sheltered the settling that is not covered by biozeolite, delays pollutent and is discharged into overlying water, has also weakened the bottom-dwelling perturbation action simultaneously; (2) physics is sheltered and settling is discharged pollutent is put together, and has improved Pollutant levels, thereby has strengthened the adsorptive power of zeolite to pollutent; (3) duration of contact of prolongation pollutent and biozeolite, physics, chemistry, the biological action of biozeolite have been strengthened.The effect of biozeolite layer: (1) zeolite quick exchange settling discharges ammonia nitrogen; (2) the solvability orthophosphoric acid salt that discharges of the stationary cation settlings such as calcium magnesium that come out of zeolite exchange; (3) partial nitrobacteria or ammonia oxidizing bacteria are converted into nitrite nitrogen or nitre nitrogen process promotion zeolite desorb ammonia nitrogen with ammonia nitrogen, make and Zeolite-bio regeneration improve its long-term effectiveness; (4) organism is denitrogenated and cut down to partial biological denitrification; (5) zeolite is to certain adsorption effect that has of organism, Fe, Mn.
Two, the biological activity multilayer covers and repairs the research of polluted by nitrogen settling effect
(1) testing program
Testing apparatus is that DN200, height are the synthetic glass post of 1.0m, and thickness of deposits is 30cm, and overlying water is 50cm deeply.
Test has 7 synthetic glass posts, is numbered 1～7#, is respectively: 1#, blank; 2#, biozeolite covers, and covering intensity is 2kgm -2, BZ2; 3#, biozeolite covers, and covering intensity is 3kgm -2, BZ3; 4#, biozeolite cover (abbreviating combination as covers) in last multilayer at following fine sand, it is 2kgm that zeolite covers intensity -2, it is 1kgm that fine sand covers intensity -2, BZ2+FS1; 5#, combination covers, and it is 2kgm that zeolite covers intensity -2, it is 3kgm that fine sand covers intensity -2, BZ2+FS3; 6#, combination covers, and it is 2kgm that zeolite covers intensity -2, it is 5kgm that fine sand covers intensity -2, BZ2+FS5; 7#, combination covers, and it is 2kgm that zeolite covers intensity -2, it is 10kgm that fine sand covers intensity -2, BZ2+FS10.
Study biological reactive multilayer and cover reparation polluted by nitrogen settling technical (effect analysis) and economy (technical costs).
(2) test-results and analysis
1. multilayer covers with individual layer and covers reduction overlying water nitrogen load effect
In the process of the test in the overlying water water temperature be that 22～27 ℃, pH7～8, DO are 1～4mgL -1(under the natural condition), test lasts 54d; TN, NH in BZ2, BZ3 and BZ2+FS10 covering system and the blank system overlying water 4 +-N, NO 2 --N, NO 3 -The variation of-N concentration is respectively like Fig. 5, Fig. 6, Fig. 7, shown in Figure 8.
Visible by Fig. 5, test lasts 54d, compares with blank, and BZ2, BZ3 and BZ2+FS10 covering system are 7.78%, 59.63% and 67.59% to overlying water TN concentration body reduction rate; Identical biozeolite covers under the strength condition, and biozeolite covers than independent biozeolite covering with the river sand multilayer overlying water TN concentration reduction rate has been improved 60%; BZ2+FS10 multilayer covering system has improved 8% to overlying water TN concentration reduction rate than BZ3, explains that the multilayer soverlay technique can reduce the dosage of zeolite, has practiced thrift technical costs, is a kind of cost-effective polluted by nitrogen settling recovery technique.
Visible by Fig. 6, test lasts 14d, and blank system settling continues to discharge ammonia nitrogen, compares with blank, and BZ2, BZ3 and BZ2+FS10 covering system are to overlying water NH 4 +-N concentration body reduction rate is 58.99%, 57.47% and 52.62%, and BZ2, BZ3 and BZ2+FS10 cut down effect to the overlying water ammonia nitrogen and differ not obvious.
Visible by Fig. 7, nitrite nitrogen integration time is about 20d in each system.
Visible by Fig. 8, test lasts 54d, compares with blank, and BZ2, BZ3 and BZ2+FS10 covering system are to NO in the overlying water 3 --N concentration reduction rate is 41.54%, 83.38% and 79.53%; NO in 2 pairs of overlying waters of BZ2+FS10 3 --N concentration reduction rate has improved 38% than BZ2, and the upper strata river sand covers the biological denitrification denitrogenation of enhanced biological zeolite.
2. different intensity river sand and the biozeolite multilayer covering reduction overlying water nitrogen load effects of covering
In the process of the test in the overlying water water temperature be that 22～27 ℃, pH7～8, DO are 1～4mgL -1(under the natural condition), test lasts 54d; TN, NH in BZ2+FS1, BZ2+FS3, BZ2+FS5 and BZ2+FS10 covering system and the blank system overlying water 4 +-N, NO 2 --N, NO 3 -The variation of-N concentration is respectively like Fig. 9, Figure 10, Figure 11, shown in Figure 12.
Visible by Fig. 9, test lasts 54d, compares with blank, and BZ2+FS1, BZ2+FS3, BZ2+FS5 and BZ2+FS10 cover the TN concentration reduction rate in the overlying water is respectively 39.44%, 52.41%, 33.89% and 67.59%; Along with the increase that river sand covers intensity, multilayer covers has increase trend to TN reduction effect in the overlying water, but dependency is not obvious, and BZ2+FS10 multilayer coverage effect is best.
Visible by Figure 10, compare with blank, BZ2+FS1, BZ2+FS3, BZ2+FS5 and BZ2+FS10 cover has good reduction effect to the overlying water ammonia nitrogen, differs not obvious but cut down effect.
Visible by Figure 11, about the integration time of the nitrate nitrogen of each system about 20d.
Visible by Figure 12, test lasts 54d, compares with blank, and BZ2+FS1, BZ2+FS3, BZ2+FS5 and BZ2+FS10 cover the NO in the overlying water 3 --N concentration reduction rate is respectively 68.25%, 77.75%, 55.19% and 79.53%; Along with the increase that river sand covers intensity, multilayer covers NO in the overlying water 3 --N cuts down effect has increase trend, but dependency is not obvious, and BZ2+FS10 multilayer coverage effect is best.

Claims (3)

1. a pollution deposit microbic activity multilayer covers restorative procedure, and it is characterized in that: this method may further comprise the steps:
1) select using natural clinoptilolite and river sand as the Marine sediments covering material;
2) high-efficiency strain enrichment: screening is obtained nitrobacteria and denitrifying bacterium is inoculated in respectively in nitrobacteria liquid nutrient medium and the denitrifying bacterium liquid nutrient medium; 2-3d is cultivated in vibration under 25-30 ℃, 120rpm constant temperature, obtains enrichment nitrobacteria and denitrifying bacterium bacterium liquid respectively;
The prescription of described nitrobacteria substratum is: sodium acetate 0.3gL -1, potassium hydrogenphosphate 0.02gL -1, magnesium chloride 0.05gL -1, ammonium chloride 0.06gL -1, liquid microelement 2mL; Wherein micro-liquid formula is: with EDTA 50g, ZnSO 42.2g, CaCl 25.5g, MnCl 24H 2O 5.06g, FeSO 47H 2O 5.0g, ammonium molybdate 1.1g, CuSO 45H 2O 1.57g, CoCl 26H 2O 1.61g joins in the 1000mL deionized water, uses 1molL again -1HCl and 1molL -1NaOH regulates pH=7;
The prescription of described denitrifying bacterium substratum is: sodium acetate 0.3gL -1, potassium hydrogenphosphate 0.02gL -1, magnesium chloride 0.05gL -1, SODIUMNITRATE 0.06gL -1, liquid microelement 2mL, wherein liquid microelement is formulated as: with EDTA 50g, ZnSO 42.2g, CaCl 25.5g, MnCl 24H 2O 5.06g, FeSO 47H 2O 5.0g, ammonium molybdate 1.1g, CuSO 45H 2O1.57g, CoCl 26H 2O 1.61g joins in the 1000mL deionized water, uses 1molL again -1HCl and 1molL -1NaOH regulates pH=7;
3) zeolite microorganism colonization: at first former water is sterilized in High pressure steam sterilizer, wherein sterilising conditions is 120～126 ℃ of temperature, pressure 0.10～0.14MPa; Time 30min, nitrobacteria that then enrichment is obtained and the bacterium liquid of denitrifying bacterium are to carry out proportioning at 1: 1 to obtain mixed bacteria liquid according to volume ratio, are to add to former water at 1: 9 mixed bacteria liquid and former water according to volume ratio then; Constant temperature water bath vibration 2～3d under 25-30 ℃, 120rpm condition; After former water becomes muddiness, the former water of muddiness is poured in the device that fills the zeolite appearance, muddy former water liquid level exceeds zeolite 5cm and gets final product; Hanging film is controlled at the concentration of DO through aerating apparatus and quickens to hang the film process more than the 2-3mg/L; After continuous aeration is hung film 3d, adopt solid stream of water to wash out the bacterium of the absorption of filling surface, obtain biozeolite;
4) covering material adds: at last multilayer coverage mode covering material is evenly covered and carry out biological prosthetic on the settling at following, river sand according to biozeolite.
2. pollution deposit microbic activity multilayer as claimed in claim 1 covers restorative procedure, and it is characterized in that: described using natural clinoptilolite particle diameter is 1～2mm, and covering intensity is 2～3kg/m 2, cladding thickness is 2～3mm, and the particle diameter of river sand is 0.15～0.20mm, and covering intensity is 10kg/m 2, cladding thickness is 1.1cm.
3. pollution deposit microbic activity multilayer as claimed in claim 1 covers restorative procedure, and it is characterized in that: described nitrobacteria is pseudomonas SY1 (Pseudomonas sp.SY1), deposit number CCTCCNO:M 209181; Described denitrifying bacterium is pseudomonas HY1 (Pseudomonas sp.HY1), deposit number CCTCCNO:M 209180.
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CN103288217A (en) * 2013-05-28 2013-09-11 长沙理工大学 Method for controlling sediment siltation after dredging
CN104129894A (en) * 2014-07-02 2014-11-05 西安建筑科技大学 Method for in-situ continuous restoration of serious contamination sediment by covering single layer modified membrane-forming zeolite
CN104150724A (en) * 2014-07-02 2014-11-19 西安建筑科技大学 Method for prolonging service life of active zeolite covering layer
CN104150724B (en) * 2014-07-02 2016-01-20 西安建筑科技大学 A kind of method extending the activated zeolite tectum life-span
CN104129894B (en) * 2014-07-02 2016-03-30 西安建筑科技大学 Cover the method that monolayer-modified biofilm zeolite in-situ continues to repair serious pollution bed mud
CN107500929A (en) * 2017-10-10 2017-12-22 南京仙草堂生物科技有限公司 A kind of biological organic fertilizer for suppressing soil disease and preparation method thereof and application process
CN110217898A (en) * 2019-07-11 2019-09-10 西安建筑科技大学 A kind of filler of rural domestic sewage treatment and the bacteria agent of biofilm
CN110217898B (en) * 2019-07-11 2022-03-04 西安建筑科技大学 Biological agent for rural domestic sewage treatment, biofilm formation filler, preparation method of filler and biofilm formation method

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