CN102531197B - Bioactive multilayer covering remediation method for contaminated sediment - Google Patents
Bioactive multilayer covering remediation method for contaminated sediment Download PDFInfo
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- CN102531197B CN102531197B CN201110450470.4A CN201110450470A CN102531197B CN 102531197 B CN102531197 B CN 102531197B CN 201110450470 A CN201110450470 A CN 201110450470A CN 102531197 B CN102531197 B CN 102531197B
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Abstract
The invention discloses a bioactive multilayer covering remediation method for contaminated sediments. Bioactive multilayer covering with a synergistic effect of river sand and natural zeolite for fixing high-efficiency nitrifying bacteria and denitrifying bacteria is adopted for reducing the amount of contaminants released towards a water body in the sediments and controlling the eutrophication of the water body. The method comprises the steps of: using the river sand and the biozeolite as covering materials, inoculating highly-effective strain bacteria solution obtained through screening into liquid culture medium, adding enriched nitrifying bacteria and denitrifying bacteria liquid into sterilized eutrophic raw water, pouring turbid raw water into a container containing a certain amount of zeolite after the raw water becomes turbid, forming a biofilm, controlling the concentration of dissolved oxygen (DO) to be 2-3mg/L through an aeration device to accelerate biofilm formation, and after three days of continuous aeration for biofilm formation, using continuous water to wash away bacteria which are adsorbed on the surfaces of fillers. The prepared biozeolite and the clean river sand evenly cover the sediments in a multilayer covering way of laying the biozeolite at a lower layer and the river sand at an upper layer. Therefore, a bioactive multilayer covering technique is used for realizing the in-situ remediation of the contaminated sediments.
Description
Technical field
The invention belongs to water-treatment technology field, be specifically related to a kind of technology that adopts biological activity multilayer cover to repair Marine sediments, be specially adapted to the reparation of serious river, lake and reservoir sediments of endogenous nitrogen, phosphorus load.
Background technology
Body eutrophication is the difficult problem that current water pollution is administered, and has become one of global most important environmental problem.Settling is N in water body, P " source " or " remittance ", and N, P concentration are the main limiting factor of body eutrophication.In the situation that external source N, P are effectively controlled, the release of controlling the endogenous N of settling, P will become the effective measure of controlling body eutrophication.The method of cutting down at present Marine sediments internal loading has the methods such as Natural Attenuation, dredging and covering.Natural Attenuation method is applicable to source of pollution and is effectively controlled, and deposition and diluting effect environment faster.Dredging method mainly have problems settling suspend again to water body pollute, transporting sediments and treating processes causes secondary pollution, expense is high and to destruction of water body biology community structure etc.Cladding process is world today's study hotspot, physics masking layer by traditional thick develops into current thin active tectum, the active cladding process existing problems of thin layer have: after active covering material is saturated to pollutent absorption, will lose ability to function, its long-term effectiveness has much room for improvement; In practical application, inhomogeneous covering makes part settling capped, causes sediment pollution thing to be directly released into water body and the release of bottom-dwelling disturbance promotion sediment pollution thing.
Summary of the invention
For prior art, there is defect or deficiency, the object of the invention is to, a kind of bioactive multilayer covering remediation method for contaminated sediment is provided, can effectively cut down the pollutents such as nitrogen in Marine sediments, phosphorus and organism to the burst size of water body, strengthen tectum long-term effectiveness, reduce running cost, control body eutrophication.
In order to realize above-mentioned technical assignment, the present invention adopts following technical scheme to be achieved:
1) select using natural clinoptilolite and river sand as Marine sediments covering material;
2) high-efficiency strain enrichment: screening is obtained to nitrobacteria and denitrifying bacterium is inoculated in respectively in nitrobacteria liquid nutrient medium and denitrifying bacterium liquid nutrient medium, under 25-30 ℃, 120rpm constant temperature, 2-3d is cultivated in vibration, obtains respectively enrichment nitrobacteria and denitrifying bacterium bacterium liquid.
The formula of described nitrobacteria substratum is: sodium acetate 0.3gL
-1, dipotassium hydrogen phosphate 0.02gL
-1, magnesium chloride 0.05gL
-1, ammonium chloride 0.06gL
-1, liquid microelement 2mL; Wherein micro-liquid formula is: by EDTA 50g, ZnSO
42.2g, CaCl
25.5g, MnCl
24H
2o 5.06g, FeSO
47H
2o 5.0g, ammonium molybdate 1.1g, CuSO
45H
2o 1.57g, CoCl
26H
2o 1.61g, joins in 1000mL deionized water, then uses 1molL
-1hCl and 1molL
-1naOH regulates pH=7;
The formula of described denitrifying bacterium substratum is: sodium acetate 0.3gL
-1, dipotassium hydrogen phosphate 0.02gL
-1, magnesium chloride 0.05gL
-1, SODIUMNITRATE 0.06gL
-1, liquid microelement 2mL, wherein liquid microelement is formulated as: by EDTA 50g, ZnSO
42.2g, CaCl
25.5g, MnCl
24H
2o 5.06g, FeSO
47H
2o 5.0g, ammonium molybdate 1.1g, CuSO
45H
2o1.57g, CoCl
26H
2o 1.61g, joins in 1000mL deionized water, then uses 1molL
-1hCl and 1molL
-1naOH regulates pH=7;
3) zeolite microorganism colonization: first former water is carried out in High pressure steam sterilizer to sterilizing, sterilising conditions is 120～126 ℃ of temperature, pressure 0.10～0.14MPa, time 30min, then the bacterium liquid of nitrobacteria enrichment being obtained and denitrifying bacterium is to carry out proportioning at 1: 1 to obtain mixed bacteria liquid according to volume ratio, then by mixed bacteria liquid and former water, according to volume ratio, be to add at 1: 9 to former water, at 25-30 ℃, 120rpm Water Under is bathed constant temperature oscillation 2～3d, former water becomes after muddiness, the former water of muddiness is poured in the device that fills zeolite appearance, muddy former water liquid level exceeds zeolite 5cm, biofilm is controlled at 2-3mg/L by aerating apparatus by the concentration of DO and accelerates above biofilm process, after continuous aeration biofilm 3d, adopt solid stream of water to wash out the bacterium of the absorption of filling surface, obtain biozeolite,
4) covering material adds: according to biozeolite under, river sand carries out covering material uniform fold biological restoration on settling at upper multilayer coverage mode.
Other technologies feature of the present invention is: described using natural clinoptilolite particle diameter is 1～2mm, and coverage strength is 2～3kg/m
2, cladding thickness is 2～3mm, and the particle diameter of river sand is 0.15～0.20mm, and coverage strength is 10kg/m
2, cladding thickness is 1.1cm.
Bioactive multilayer covering remediation method for contaminated sediment of the present invention, can effectively cut down the pollutents such as nitrogen in Marine sediments, phosphorus and organism to the burst size of water body, strengthens tectum long-term effectiveness, reduces running cost, controls body eutrophication.The present invention compared with prior art has following technological merit: (1) effectively cuts down the pollutents such as nitrogen in settling, phosphorus and organism to the burst size of water body, Fe, Mn is also had to certain inhibition simultaneously; (2) bio-regeneration of zeolite strengthens tectum long-term effectiveness; (3) reduce biozeolite coverage strength, reduce technical costs; (4) control body eutrophication.
Accompanying drawing explanation
Fig. 1-Fig. 4 is that experimental verification biozeolite biozeolite of the present invention and river sand various combination mode cover reparation Marine sediments effect experimental result picture, and wherein Fig. 1 is TN change in concentration figure in different system overlying water, and Fig. 2 is NH in different system overlying water
4 +-N change in concentration figure, Fig. 3 is NO in different system overlying water
2 --N change in concentration figure, Fig. 4 is NO in different system overlying water
3 --N change in concentration figure.
Fig. 5-Fig. 8 is that multilayer of the present invention covers and individual layer covering reduction overlying water nitrogen load effect experiment effect figure, and wherein Fig. 5 is the variation diagram of TN concentration in different system overlying water, and Fig. 6 is NH in different system overlying water
4 +the variation diagram of-N concentration; Fig. 7 is NO in different system overlying water
2 -the variation diagram of-N concentration, Fig. 8 is NO in different system overlying water
3 --N change in concentration figure.
To be the different coverage strength river sands of the present invention cover and cut down the overlying water nitrogen design sketch of loading with biozeolite multilayer Fig. 9-Figure 12, the variation diagram of TN concentration in Fig. 9 different system overlying water wherein, and Figure 10 is NH in different system overlying water
4 +the variation diagram of-N concentration; Figure 11 is NO in different system overlying water
2 -the variation diagram of-N concentration, Figure 12 is NO in different system overlying water
3 --N change in concentration figure.
Below in conjunction with accompanying drawing, particular content of the present invention is described in further detail.
Embodiment
The present invention relates to the high-efficiency strain of screening for screening and obtain nitrobacteria SY1 (pseudomonas SY1) and denitrifying bacterium HY1 (pseudomonas HY1) from Heihe Reservoir bed mud, this two bacterial classification is preserved in Chinese Typical Representative culture collection center on August 24th, 2009, be called for short CCTCC, and register on the books, this biological inoculum was preserved 30 years in 24 days Augusts in 2009.Nitrobacteria pseudomonas SY1 (Pseudomonas sp.SY1) wherein, preserving number is: CCTCCNO:M 209181; Denitrifying bacterium pseudomonas HY1 (Pseudomonas sp.HY1) preserving number is: CCTCCNO:M 209180.
Biological activity multilayer of the present invention covers the Marine sediments technology of repairing, its adopts covering material is the i.e. fixing natural zeolite of High-efficient Nitrobacteria and denitrifying bacterium (being called biozeolite) of river sand and biological activity covering material, coverage mode be biozeolite under, river sand is upper.
Bioactive multilayer covering remediation method for contaminated sediment of the present invention, the method comprises the following steps:
1) select using natural clinoptilolite and river sand as Marine sediments covering material, wherein select using natural clinoptilolite as microbial fixed carrier.Select using natural clinoptilolite, its advantage has specific surface area large, porous, easy to be filmed, can create the anaerobism of suitable microorganism growth, facultative and aerobic varying environment, ammonium ion is had to good exchange capacity, and ammonium adsorptive power can bio-regeneration, and physical and chemical performance is stable, nontoxicity, hydraulics is abundant, cheap, and the place of production is abundant.The using natural clinoptilolite particle diameter that the present invention selects is 1～2mm, and coverage strength is 2～3kg/m
2, cladding thickness is 2～3mm.
Select with river sand, its advantage physical and chemical performance is stable, nontoxicity, and masking performance is strong, cheap, and the source of goods is wide.The particle diameter of the river sand that the present invention selects is 0.15～0.20mm, and coverage strength is 10kg/m
2, cladding thickness is 1.1cm.
2) high-efficiency strain enrichment: screening is obtained to nitrobacteria and denitrifying bacterium is inoculated in respectively in nitrobacteria liquid nutrient medium and denitrifying bacterium liquid nutrient medium, under 25-30 ℃, 120rpm constant temperature, 2-3d is cultivated in vibration, obtains respectively enrichment nitrobacteria and denitrifying bacterium bacterium liquid.
Wherein screening obtains nitrobacteria denitrifying bacterium and is from Heihe Reservoir bed mud screening to obtain nitrobacteria be pseudomonas SY1 (Pseudomonas sp.SY1), deposit number CCTCCNO:M 209181 and denitrifying bacterium are pseudomonas HY1 (Pseudomonas sp.HY1), deposit number CCTCCNO:M 209180 is inoculated in respectively in nitrobacteria liquid nutrient medium and denitrifying bacterium liquid nutrient medium, 25-30 ℃, 120rpm constant temperature oscillation cultivation 2-3d;
Wherein nitrobacteria SY1 and denitrifying bacterium HY1 bacterial strain all belong to Rhodopseudomonas, and it has following feature separately:
(a) colony morphology characteristic and morphological features are referring to table 2.
Table 2
(b) major physiological, biochemical character, referring to table 3:
Table 3
(note: √ expresses support for ,+represent growth or react positive)
(c) NH
4 +-N, NO
3 ---N clearance
Ammonia nitrogen starting point concentration is 1.26mg/L, and after the cultivation of 48h, ammonia nitrogen ultimate density is 0.0112mg/L, to ammonia nitrogen removal frank, is 99.11%.
Nitrate starting point concentration is 2.3mg/L, and after the cultivation of 48h, nitrate ultimate density is 0.041mg/L, to nitrate removal rate, is 98.52%.Referring to table 4:
| Can bacterial strain | Starting point concentration (mg/L) | Ultimate density (mg/L) | Clearance (%) |
| SY1 | 1.26 | 0.0112 | 99.11 |
| HY1 | 2.3 | 0.041 | 98.52 |
Table 4
Wherein the formula of nitrobacteria pseudomonas SY1 substratum is: sodium acetate 0.3gL
-1, dipotassium hydrogen phosphate 0.02gL
-1, magnesium chloride 0.05gL
-1, ammonium chloride 0.06gL
-1, liquid microelement 2mL; Wherein micro-liquid formula is: EDTA 50g, ZnSO
42.2g, CaCl
25.5g, MnCl
24H
2o 5.06g, FeSO
47H
2o 5.0g, ammonium molybdate 1.1g, CuSO
45H
2o1.57g, CoCl
26H
2o 1.61g, joins in 1000mL deionized water, then uses 1molL
-1hCl and 1molL
-1naOH regulates pH=7;
The formula of denitrifying bacterium pseudomonas HY1 substratum is: sodium acetate 0.3gL
-1, dipotassium hydrogen phosphate 0.02gL
-1, magnesium chloride 0.05gL
-1, SODIUMNITRATE 0.06gL
-1, liquid microelement 2mL, wherein liquid microelement is formulated as: EDTA 50g, ZnSO
42.2g, CaCl
25.5g, MnCl
24H
2o 5.06g, FeSO
47H
2o 5.0g, ammonium molybdate 1.1g, CuSO
45H
2o1.57g, CoCl
26H
2o 1.61g, joins in 1000mL deionized water, then uses 1molL
-1hCl and 1molL
-1naOH regulates pH=7.
3) zeolite microorganism colonization: first former water is carried out in High pressure steam sterilizer to sterilizing, sterilising conditions is 120～126 ℃ of temperature, pressure 0.10～0.14MPa, time 30min, then the bacterium liquid of nitrobacteria enrichment being obtained and denitrifying bacterium is to carry out proportioning at 1: 1 to obtain mixed bacteria liquid according to volume ratio, then by mixed bacteria liquid and former water, according to volume ratio, be to add at 1: 9 to former water, at 25-30 ℃, 120rpm Water Under bath constant temperature oscillation 2～3d.Former water becomes after muddiness, the former water of muddiness is poured in the device that fills zeolite appearance, muddy former water liquid level exceeds zeolite 5cm, biofilm is controlled at 2-3mg/L by aerating apparatus by the concentration of DO and accelerates above biofilm process, after continuous aeration biofilm 3d, adopt solid stream of water to wash out the bacterium of the absorption of filling surface, obtain biozeolite.
4) covering material adds: according to biozeolite under, river sand is at upper multilayer coverage mode, covering material uniform fold, on settling, is realized and Marine sediments are carried out to biological activity multilayer is covered and repair.
Experimental verification:
In accordance with above-mentioned steps of the present invention and technical essential, it is research object that the former water of Grand Canal in Yangzhou and the surface deposit of eutrophication take in the present invention, has carried out respectively little, middle experimental study in laboratory.
One, the multilayer coverage mode of biozeolite and river sand the best is determined
(1) testing program
Test is at DN200,, carry out in 10L wide-mouth vial; The overlying water depth of water is 15cm (volume is 4.17L), and thickness of deposits is 10cm (quality is about 4912g).Biozeolite and river sand array mode have: combine one, biozeolite is upper, river sand under; Combination two, biozeolite under, river sand is upper; According to river sand particle diameter, river sand is divided into rough sand (0.2～2mm) and fine sand (0.15～0.2mm).
Test is provided with 6 vials altogether, is respectively blank, biozeolite and covers that (coverage strength is 5kgm
-2, thickness is 5mm), rough sand combination one covers that (rough sand coverage strength is 23kgm
-2, thickness is 2cm; Biozeolite coverage strength is 3kgm
-2, thickness is 3mm), rough sand combination two covers (parameter is unified on the same group), fine sand combination one and covers that (fine sand coverage strength is 18.6kgm
-2, thickness is 2cm; Biozeolite coverage strength is 3kgm
-2, thickness is 3mm) and fine sand combination two coverings (parameter is unified on the same group).
Relatively biozeolite and river sand various combination mode cover and repair Marine sediments effect, determine best array mode, propose biological activity multilayer soverlay technique concrete grammar.
(2) test results and analysis
1. cut down overlying water nitrogen load effect
In process of the test, in overlying water, water temperature is that 16～20 ℃, pH7～8, DO are 2.5～6mgL
-1(under natural condition), test lasts 121d; TN, NH in each system overlying water
4 +-N, NO
2 --N, NO
3 -the variation of-N concentration respectively as shown in Figure 1, Figure 2, Figure 3, Figure 4.
As shown in Figure 1, test lasts 121d, compares with blank, and rough sand combination one, rough sand combination two, fine sand combination one and fine sand combination two cover the reduction rate of TN in overlying water is respectively to 17.59%, 33.91%, 30.93% and 52.32%; Combination two covers TN reduction rate has been improved to 16.32%～21.39% than combination one; Fine sand combination covers TN reduction rate has been improved to 13.34%～18.41% than rough sand; It is suitable to TN reduction rate with biozeolite covering that fine sand combination two covers, and illustrates under the equal reduction rate condition to TN, and fine sand combination two covers and can greatly reduce biozeolite overlay capacity, has reduced technical costs.Variance analysis shows, each covering system is cut down with blank and is had notable difference (P < 0.05) TN in overlying water, biozeolite with combination two, two couples of TN of rough sand combination two and fine sand combination subdue all has notable difference (P < 0.05), biozeolite with combination one, rough sand combination one with fine sand combination one, combine one and subdue equal no significant difference (P > 0.05) with two couples of TN of combination.
As shown in Figure 2,24d before test, NH in blank system settling
4 +-N sustained release, on average discharging flux is 102.25mgm
-2d
-1, NH in each covering system
4 +the average burst size of-N is negative value, and (except rough sand combination two coverings, its value is 0.27mgm
-2d
-1), illustrate that the covering of biological activity multilayer has suppressed NH in settling completely
4 +-N discharges.
As seen from Figure 3, compare NO in each covering system with blank
2 --N has shortened nearly 60d integration time, illustrates that the nitrification of biozeolite is obvious, in advance by NO in water body
2 --N is converted into NO
3 --N.
As seen from Figure 4, test lasts 121d, compares with blank, and rough sand combination one, rough sand combination two, fine sand combination one and fine sand combination two cover NO in overlying water
3 -the reduction rate of-N is respectively 21.35%, 73.98%, 24.59% and 73.30%; Combination two covers NO
3 --N reduction rate has improved 48.71%～52.63% than combination one; Fine sand combination covers with rough sand combination and covers NO
3 --N reduction rate is suitable.
In sum, best multilayer coverage mode be biozeolite at lower thin river sand upper.
2. biological activity multilayer covers and repairs polluted by nitrogen settling Analysis on Mechanism
As seen from Figure 1, in blank, TN concentration starts to rise gradually, and 34d reaches peak value, is reduced to gradually subsequently certain certain value and tends towards stability, and illustrates that Grand Canal in Yangzhou water body has certain self-purification capacity.
Biozeolite, rough sand combination one and fine sand combine the front 10d of TN concentration in a covering system and decline rapidly, tend towards stability subsequently, until after 24d, TN concentration ascendant trend, after 34d, in biozeolite covering system, TN concentration has rising and tends towards stability, but thick, fine sand combines TN fast rise in a covering system, after 60d, tends towards stability; Rough sand combination two and fine sand combine in two covering systems TN concentration to start 3d and slightly declines, and tend towards stability subsequently, but fine sand combines two covering systems after 30d, and TN concentration has slowly downtrending gradually, then after trend stability.
Analyze its reason, river sand combines in a covering system, initial stage lower floor's river sand covers temporary transient physics and shelters the release of settling nitrogen pollutant, ammonia nitrogen in the biozeolite quick adsorption overlying water of upper strata, make TN concentration fast-descending in water body, but after 34d, high concentration N pollutes and has penetrated river sand tectum (this is also the hidden danger of independent husky soverlay technique maximum), biozeolite is less than sand bed polluted by nitrogen releasability to the adsorptive power of nitrogen, makes TN concentration fast rise in water body.
And river sand combines in two covering systems, the a small amount of nitrogen pollutant of initial stage upper strata river sand tectum absorption overlying water, make TN in overlying water slightly reduce again and tend towards stability, lower floor's biozeolite tectum quick adsorption settling discharges nitrogen pollutant, husky tectum physical barriers has strengthened polluted by nitrogen concentration, (test lasts 121d, and rough sand combination one, rough sand combination two, fine sand combination and fine sand combination two system mesolite ammonia nitrogen absorption amount are respectively 1.24,2.99,1.16 and 3.14mgg to have improved biozeolite adsorption of nitrogen pollutent ability
-1), and upper strata river sand layer extends the duration of contact of pollutent and biozeolite, physics, chemistry, the biological action of biozeolite have been strengthened, reduce husky tectum polluted by nitrogen concentration, when in overlying water, N concentration is higher than husky tectum, overlying water N pollutent moves to sand bed, removed gradually, thereby in overlying water, TN concentration has downtrending gradually by biozeolite physical and chemical adsorption and biological denitrification.
The mechanism of action that visible biological activity multilayer covers reparation Marine sediments is: by lower floor's biozeolite and upper strata river sand synergy, repair Marine sediments; River sand layer Main Function: (1) physics is sheltered the settling not covered by biozeolite, delays pollutent and is discharged into overlying water, has also weakened bottom-dwelling perturbation action simultaneously; (2) physics is sheltered and settling is discharged to pollutent is put together, and has improved Pollutant levels, thereby has strengthened the adsorptive power of zeolite to pollutent; (3) extend the duration of contact of pollutent and biozeolite, strengthened physics, chemistry, the biological action of biozeolite.Biozeolite layer effect: (1) zeolite exchanges fast settling and discharges ammonia nitrogen; (2) the solvability orthophosphoric acid salt that the positively charged ion immobilized sediment such as zeolite exchange calcium magnesium out discharges; (3) local nitrobacteria or ammonia oxidizing bacteria are that nitrite nitrogen or nitre nitrogen process promote zeolite desorb ammonia nitrogen by mineralized nitrogen, make Zeolite-bio regeneration, improve its long-term effectiveness; (4) organism is denitrogenated and cut down to local biological denitrification; (5) the have certain adsorption effect of zeolite to organism, Fe, Mn.
Two, biological activity multilayer covers and repairs the research of polluted by nitrogen settling effect
(1) testing program
Testing apparatus is that DN200, height are the synthetic glass post of 1.0m, and thickness of deposits is 30cm, and overlying water is 50cm deeply.
Test has 7 synthetic glass posts, is numbered 1～7#, is respectively: 1#, blank; 2#, biozeolite covers, and coverage strength is 2kgm
-2, BZ2; 3#, biozeolite covers, and coverage strength is 3kgm
-2, BZ3; 4#, biozeolite covers (referred to as combination, covering) in upper multilayer at lower fine sand, and zeolite coverage strength is 2kgm
-2, fine sand coverage strength is 1kgm
-2, BZ2+FS1; 5#, combination covers, and zeolite coverage strength is 2kgm
-2, fine sand coverage strength is 3kgm
-2, BZ2+FS3; 6#, combination covers, and zeolite coverage strength is 2kgm
-2, fine sand coverage strength is 5kgm
-2, BZ2+FS5; 7#, combination covers, and zeolite coverage strength is 2kgm
-2, fine sand coverage strength is 10kgm
-2, BZ2+FS10.
Study biological reactive multilayer and cover reparation polluted by nitrogen settling technical (effect analysis) and economy (technical costs).
(2) test results and analysis
1. multilayer covers with individual layer and covers and cut down overlying water nitrogen load effect
In process of the test, in overlying water, water temperature is that 22～27 ℃, pH7～8, DO are 1～4mgL
-1(under natural condition), test lasts 54d; TN, NH in BZ2, BZ3 and BZ2+FS10 covering system and blank system overlying water
4 +-N, NO
2 --N, NO
3 -the variation of-N concentration is respectively as shown in Fig. 5, Fig. 6, Fig. 7, Fig. 8.
As seen from Figure 5, test lasts 54d, compares with blank, and BZ2, BZ3 and BZ2+FS10 covering system are 7.78%, 59.63% and 67.59% to overlying water TN concentration body reduction rate; Under identical biozeolite coverage strength condition, biozeolite covers than independent biozeolite and covers overlying water TN concentration reduction rate has been improved to 60% with river sand multilayer; BZ2+FS10 multilayer covering system has improved 8% to overlying water TN concentration reduction rate than BZ3, illustrates that multilayer soverlay technique can reduce the dosage of zeolite, has saved technical costs, is a kind of cost-effective polluted by nitrogen sediment remediation technology.
As seen from Figure 6, test lasts 14d, and blank system settling sustained release ammonia nitrogen, compares with blank, and BZ2, BZ3 and BZ2+FS10 covering system are to overlying water NH
4 +-N concentration body reduction rate be 58.99%, 57.47% and 52.62%, BZ2, BZ3 and BZ2+FS10 overlying water ammonia nitrogen cut down to effect differ not obvious.
As seen from Figure 7, in each system, nitrite nitrogen is 20d left and right integration time.
As seen from Figure 8, test lasts 54d, compares with blank, and BZ2, BZ3 and BZ2+FS10 covering system are to NO in overlying water
3 --N concentration reduction rate is 41.54%, 83.38% and 79.53%; NO in 2 pairs of overlying waters of BZ2+FS10
3 --N concentration reduction rate has improved 38% than BZ2, and upper strata river sand covers the biological denitrification denitrogenation of enhanced biological zeolite.
2. different coverage strength river sands and biozeolite multilayer cover and cut down overlying water nitrogen load effect
In process of the test, in overlying water, water temperature is that 22～27 ℃, pH7～8, DO are 1～4mgL
-1(under natural condition), test lasts 54d; TN, NH in BZ2+FS1, BZ2+FS3, BZ2+FS5 and BZ2+FS10 covering system and blank system overlying water
4 +-N, NO
2 --N, NO
3 -the variation of-N concentration is respectively as shown in Fig. 9, Figure 10, Figure 11, Figure 12.
As seen from Figure 9, test lasts 54d, compares with blank, and BZ2+FS1, BZ2+FS3, BZ2+FS5 and BZ2+FS10 cover the TN concentration reduction rate in overlying water is respectively to 39.44%, 52.41%, 33.89% and 67.59%; Along with the increase of river sand coverage strength, multilayer covers has increase trend to TN reduction effect in overlying water, but dependency is not obvious, and BZ2+FS10 multilayer coverage effect is best.
As seen from Figure 10, compare with blank, BZ2+FS1, BZ2+FS3, BZ2+FS5 and BZ2+FS10 cover has good reduction effect to overlying water ammonia nitrogen, but cut down effect, differs not obvious.
As seen from Figure 11, the about 20d integration time of the nitrate nitrogen of each system left and right.
As seen from Figure 12, test lasts 54d, compares with blank, and BZ2+FS1, BZ2+FS3, BZ2+FS5 and BZ2+FS10 cover the NO in overlying water
3 --N concentration reduction rate is respectively 68.25%, 77.75%, 55.19% and 79.53%; Along with the increase of river sand coverage strength, multilayer covers NO in overlying water
3 --N cuts down effect increase trend, but dependency is not obvious, and BZ2+FS10 multilayer coverage effect is best.
Claims (2)
1. a bioactive multilayer covering remediation method for contaminated sediment, is characterized in that: the method comprises the following steps:
1) select using natural clinoptilolite and river sand as Marine sediments covering material;
2) high-efficiency strain enrichment: screening is obtained to nitrobacteria and denitrifying bacterium is inoculated in respectively in nitrobacteria liquid nutrient medium and denitrifying bacterium liquid nutrient medium, under 25-30 ℃, 120rpm constant temperature, 2-3d is cultivated in vibration, obtains respectively enrichment nitrobacteria and denitrifying bacterium bacterium liquid; Wherein nitrobacteria is pseudomonas SY1(Pseudomonas sp.SY1), deposit number CCTCCNO:M209181; Denitrifying bacterium is pseudomonas HY1(Pseudomonas sp.HY1), deposit number CCTCCNO:M209180;
The formula of described nitrobacteria substratum is: sodium acetate 0.3gL
-1, dipotassium hydrogen phosphate 0.02gL
-1, magnesium chloride 0.05gL
-1, ammonium chloride 0.06gL
-1, liquid microelement 2mL; Wherein micro-liquid formula is: by EDTA50g, and ZnSO
42.2g, CaCl
25.5g, MnCl
24H
2o5.06g, FeSO
47H
2o5.0g, ammonium molybdate 1.1g, CuSO
45H
2o1.57g, CoCl
26H
2o1.61g, joins above-mentioned nitrobacteria substratum in 1000mL deionized water, then uses 1molL
-1hCl and 1molL
-1naOH regulates pH=7; The formula of described denitrifying bacterium substratum is: sodium acetate 0.3gL
-1, dipotassium hydrogen phosphate 0.02gL
-1, magnesium chloride 0.05gL
-1, SODIUMNITRATE 0.06gL
-1, liquid microelement 2mL, wherein micro-liquid formula is: by EDTA50g, ZnSO
42.2g, CaCl
25.5g, MnCl
24H
2o5.06g, FeSO
47H
2o5.0g, ammonium molybdate 1.1g, CuSO
45H
2o1.57g, CoCl
26H
2o1.61g, joins above-mentioned denitrifying bacterium substratum in 1000mL deionized water, then uses 1molL
-1hCl and 1molL
-1naOH regulates pH=7;
3) zeolite microorganism colonization: first former water is carried out in High pressure steam sterilizer to sterilizing, wherein sterilising conditions is 120～126 ℃ of temperature, pressure 0.10～0.14MPa, time 30min, then the bacterium liquid of nitrobacteria enrichment being obtained and denitrifying bacterium is that 1:1 carries out proportioning and obtains mixed bacteria liquid according to volume ratio, then by mixed bacteria liquid and former water, according to volume ratio, be that 1:9 adds to former water, at 25-30 ℃, 120rpm Water Under is bathed constant temperature oscillation 2～3d, former water becomes after muddiness, the former water of muddiness is poured into and filled in zeolite container, muddy former water liquid level exceeds zeolite 5cm, biofilm is controlled at 2-3mg/L by aerating apparatus by the concentration of DO and accelerates above biofilm process, after continuous aeration biofilm 3d, adopt solid stream of water to wash out the bacterium of the absorption of filling surface, obtain biozeolite,
4) covering material adds: according to biozeolite under, river sand carries out covering material uniform fold biological restoration on settling at upper multilayer coverage mode.
2. bioactive multilayer covering remediation method for contaminated sediment as claimed in claim 1, is characterized in that: described
Using natural clinoptilolite particle diameter is 1～2mm, and coverage strength is 2～3kg/m
2, cladding thickness is 2～3mm, and the particle diameter of river sand is 0.15～0.20mm, and coverage strength is 10kg/m
2, cladding thickness is 1.1cm.
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| CN104129894B (en) * | 2014-07-02 | 2016-03-30 | 西安建筑科技大学 | Cover the method that monolayer-modified biofilm zeolite in-situ continues to repair serious pollution bed mud |
| CN104150724B (en) * | 2014-07-02 | 2016-01-20 | 西安建筑科技大学 | A kind of method extending the activated zeolite tectum life-span |
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