CN108545829A - A kind of method of aquiculture sewerage original position aerobic nitrogen removal treatment and purification - Google Patents
A kind of method of aquiculture sewerage original position aerobic nitrogen removal treatment and purification Download PDFInfo
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- CN108545829A CN108545829A CN201810378221.0A CN201810378221A CN108545829A CN 108545829 A CN108545829 A CN 108545829A CN 201810378221 A CN201810378221 A CN 201810378221A CN 108545829 A CN108545829 A CN 108545829A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/30—Aerobic and anaerobic processes
- C02F3/301—Aerobic and anaerobic treatment in the same reactor
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/30—Aerobic and anaerobic processes
- C02F3/302—Nitrification and denitrification treatment
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
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Abstract
The present invention relates to sewage in-situ advanced treating purification techniques field, especially a kind of method of aquiculture sewerage original position aerobic nitrogen removal treatment and purification.Take in aquaculture pond excrement residual bait after processing with composite bacteria, antibiotic, the fatty acid ester compounded object of higher alcohols, microbial flocculant mixing fermentation culture, culture gained sediment is the microbial flocculation sediment with Heterotrophic nitrification aerobic denitrification function；Organic carbon source is added in culture gained zymotic fluid, aquiculture animal feed surface is sprayed at after mixing, obtains Heterotrophic nitrification aerobic denitrifying bacteria spraying feed；It adds after gained microbial flocculation sediment is mixed with spraying feed and is eaten for cultivation pool inner water production cultivated animals in pending aquaculture pond；Water body in aquaculture pond is adjusted, composite bacteria is added into breeding water body, and then form the heterotrophic nitrification aerobic denitrifying functional activity cenobium flco for capableing of purifying water body in water body；Using each substance of above-mentioned gained, the aerobic nitrogen removal advanced treating in situ of aquaculture pond water body, organics removal, ammonia nitrogen, nitrite nitrogen and nitrate nitrogen are realized.
Description
Technical field
The present invention relates to sewage in-situ advanced treating purification techniques field, especially a kind of aquiculture sewerage is aerobic in situ
The method of denitrogenation processing purification.
Background technology
Industrial aquaculture is the important way of Modern Fishery cultivation.Water environment pollution caused by large-scale industrialized cultivation with
The development of China's culture fishery is seriously perplexed and hampered to the problems such as aquatic products disease takes place frequently.A large amount of discharges pair of breeding wastewater
The influence of seawater fishery water area water-quality is very big, meanwhile, the offshore pollution of getting worse causes great threat to sea-farming
And harm so that industrial aquaculture water source processing cost increases.As a kind of technology-intensive industrial aquaculture pattern, cultivation water
Purified in situ treatment technology has the advantages such as ecological environment destruction is small, Tail water reuse is few, can not only protect Offshore Ecology environment, but also
The aquatic products of high yield and high quality can be obtained.Therefore, it is aquaculture field to carry out purified in situ processing to aquaculture system
One of prior development direction.Circulating water cultivation is that the inevitable choice of China's continental rise sea-farming sustainable development still at present may be used
Less for the technology of aquaculture system water process circulating and recovering, processing cost is higher, and general to nitrogen removal effect, greatly
Ground limits the popularization and application of circulating water cultivation technology.Therefore, cheap, the easy to operate aquaculture water of development cost is in situ
Advanced treating purification techniques has important research significance and actual application value.
In recent years, biological flocculation technology has certain application in aquaculture industry, which can not only realize water
The rapid translating of body ammonia nitrogen plays the role of purifying water, meanwhile, it is capable to a kind of high protein ingested as raising aquatic livestock
Bait, and then reduce feed cost.By carrying out literature search discovery to the prior art, Deng Yingneng etc. is " biological flocculation is all
Receive formation condition and function and effect in shore prawn closing culture experiment " it mentions in a text, it is cultivated in the closing of litopenaeus vannamei
Biological flocculation technology is introduced in system, the ammonia nitrogen and nitrite nitrogen concentration of water body are kept at reduced levels, and prawn survives
Rate 80% or more (《Fishery science is in progress》, 2012,33 (2):69-75)；Li Bin etc. " biological flocculation to regulating and controlling water quality make
With and imitative stichopus japonicus (Apostichopus japonicus) children's ginseng growth influence " mention in a text, add carbon source and gemma bar
Bacterium is formed by biological flocculation and can not only purify water, can more promote imitative juvenile sea cucumber growth (《Fishery science is in progress》,
2014,46 (1):197-205).But found by studying to summarize, the above technology is largely had by additionally adding
Machine carbon source only promotes ammonia nitrogen in water to generate organic nitrogen by assimilation, rather than is taken off from water to nitrogen pollutant
Except processing, and oxygen demand is high, when hypoventilation, easily causes the accumulation of ammonia nitrogen and nitrite, this disadvantage limits the skill
Extensive use of the art in water treatment field.Currently, there has been no prepare heterotrophic nitrification-aerobic denitrification activity cenobium simultaneously by culture
Flco is formed, in low consumption carbon, there is the active cenobium flco of heterotrophic nitrification-aerobic denitrification to utilize limited organic carbon
Source is by while an ammonia nitrogen part is changed into active cenobium flco organic nitrogen in aquaculture water, by remaining most nitrogens
Pollutant is changed into nitrogen and is removed, and realizes the report of breeding water body original position aerobic nitrogen removal treatment and purification.
Invention content
In view of the above-mentioned deficiencies in the prior art, it is an object of the present invention to provide a kind of purification of aquiculture sewerage in-situ treatment
Method.
To achieve the above object, the invention adopts a technical scheme as：
A kind of method of aquiculture sewerage original position aerobic nitrogen removal treatment and purification.
1) it takes excrement residual bait in aquaculture pond to be mixed after processing with wheat bran, corn flour, composite bacteria, antibiotic, height is added
Carbon polyol fatty acid ester complexes, microbial flocculant, fermented and cultured, culture gained sediment are anti-with heterotrophic nitrification-aerobic
The microbial flocculation sediment of nitrification function；Organic carbon source is added in culture gained zymotic fluid, aquaculture is sprayed at after mixing
Animal feed surface obtains heterotrophic nitrification-aerobic denitrification bacterium spraying feed；By gained microbial flocculation sediment and heterotrophism nitre
It adds after change-aerobic denitrifying bacteria spraying feed mixing and is eaten for cultivation pool inner water production cultivated animals in pending aquaculture pond,
Culture 7~10 days；
2) it is 10~20 to adjust water body C/N ratios in aquaculture pond:1；Cultivation system is fully aerated, and maintenance water body dissolved oxygen amount is
3.0~9.0mg/L adds composite bacteria into breeding water body, and then forms the heterotrophic nitrification-for capableing of purifying water body in water body
Aerobic denitrification functional activity cenobium flco；Utilize the heterotrophic nitrification-aerobic denitrification functional activity cenobium flco of above-mentioned acquisition
And heterotrophic nitrification-aerobic denitrification bacterium spraying feed and the microbial flocculation with heterotrophic nitrification-aerobic denitrification function are sunk
Starch realizes the aerobic nitrogen removal advanced treating in situ of aquaculture pond water body, organics removal, ammonia nitrogen, nitrite nitrogen and nitrate
Nitrogen.Wherein, TOC removal rates are stablized in 95% or more, TN removal rates 94% or more, and ammonia nitrogen removal frank is 92%~99%, sub-
Nitrate removal rate is 95%~100%.
The composite bacteria is heterotrophic nitrification-aerobic denitrification bacterium and bacterium for producing flocculant of microbe, step 1) compound bacteria
Kind additive amount is the 0.02%~0.20% of excrement residual bait and wheat bran, corn flour mixture quality, the inoculation of step 2) composite bacteria
Amount is 0.5 × 109cfu/m3~5.0 × 109cfu/m3；
The heterotrophic nitrification-aerobic denitrification bacterium is pseudomonad, Bacillus alcaligenes, Bacillus foecalis alkaligenes, arthrobacterium, general foster sulphur
It is one or more in coccus；The bacterium for producing flocculant of microbe is pseudomonad, Bacillus alcaligenes, Bacillus foecalis alkaligenes, pole
It is one or more in bacterium, rhodococcus erythropolis.Wherein, it is both heterotrophic nitrification-aerobic denitrification bacterium that composite bacteria, which can be selected directly,
It again can be as the dual function bacterial strain of bacterium for producing flocculant of microbe.
The step 1) takes aquaculture pond excrement residual bait mixed with wheat bran and corn flour after the disinfection of Potassium Monopersulfate disinfectant powder
Mixture is closed to obtain, the composite bacteria of mixture quality 0.02%~0.20%, addition antibiotic, high-carbon polyol fatty acid is then added
Ester complexes, it is 25~35 that mixing control reaction system water content, which is 40%~60%, C/N ratios, after addition:1, pH for 6.5~
7.5, fermented and cultured；After culture 5~10 days, microbial flocculant is added into culture, stirring makes it be uniformly mixed, and cultivates institute
It is the microbial flocculation sediment with heterotrophic nitrification-aerobic denitrification function to obtain sediment；It is added in culture gained zymotic fluid
Organic carbon source is sprayed at aquiculture animal feed surface after mixing, obtains heterotrophic nitrification-aerobic denitrification bacterium spraying feed, will
Gained microbial flocculation sediment adds after being mixed with heterotrophic nitrification-aerobic denitrification bacterium spraying feed in pending aquaculture pond
It is edible for cultivation pool inner water production cultivated animals, it cultivates 7~10 days；Wherein, antibiotic additive amount keeps antibiotic in mixture dense eventually
Degree be 10~100 μ g/L, the fatty acid ester compounded object additive amount of higher alcohols be 1.0~3.0g/kg, flocculant additive amount be 10~
100mg/L。
The fatty acid ester compounded object of higher alcohols is food industry antifoaming agent.The antibiotic is that aquaculture is common anti-
Raw element, it is specially one or more in sulphadiazine, terramycin, Florfenicol, Ofloxacin.The aquiculture animal is
One or more of fish, shrimp, shellfish, sea cucumber.
The excrement residual bait and wheat bran and corn flour in percentage terms, 70%~90% excrement residual bait, 10%~30% bran
Skin and corn flour, the wheat bran and corn flour press 1:1 mass ratio mixing.
The organic carbon source of its 1%~5wt% is added into zymotic fluid for the step 1), and it is dynamic that aquaculture is sprayed at after mixing
Object feed surface obtains heterotrophic nitrification-aerobic denitrification bacterium spraying feed；Wherein, organic carbon source be amylodextrin, it is sucrose, oligomeric
It is one or more in fructose.
It is described to have the microbial flocculation sediment of heterotrophic nitrification-aerobic denitrification function and heterotrophic nitrification-good gained
Oxygen denitrifying bacterium sprays feed according to 1:10~20 mass ratio is uniformly mixed, and as aquiculture animal mixing foodstuff, is added
In pending aquaculture pond, water body is adjusted while as aquatic animal feed.
The flocculation that the pico- biological flocculant is the flocculant NOC-1 of rhodococcus erythropolis generation, soy sauce koji mould generates
It is one or more in the flocculant PF-101 that agent AJ7002, paecilomycerol generate.
It is 10~20 that the step 2), which adds organic carbon source into aquaculture pond and adjusts water body C/N ratios,:1；The organic carbon source
It is one or more in amylodextrin, sucrose, brown sugar, oligofructose, wheat bran, corn flour.
The step 2) persistently adjusts carbon source in breeding water body, and it is 10~20 to adjust pending breeding water body C/N ratios:1；
Cultivation system is fully aerated, and maintenance water body dissolved oxygen amount is 3.0~9.0mg/L, and pH is 7.8~8.2, active cenobium flco in water body
A concentration of 0.02~0.20mL/L.Utilize above-mentioned gained heterotrophic nitrification-aerobic denitrification functional activity cenobium flco and heterotrophism
Nitrification-aerobic denitrifying bacteria spraying feed and the microbial flocculation sediment with heterotrophic nitrification-aerobic denitrification function, are realized
The aerobic nitrogen removal advanced treating in situ of aquaculture pond water body, organics removal, ammonia nitrogen, nitrite nitrogen and nitrate nitrogen.If cultivation
When activity cenobium concentration is higher in pond, organic carbon source and feed dosage are reduced, filters extra active cenobium flco.
After the completion of prepared by the aquiculture animal mixing foodstuff culture, fed at regular time and quantity into cultivation system daily mixed
Foodstuff is closed, is the 3%~10% of aquiculture animal body weight per day feeding volume, according to the type and difference of aquiculture animal
The factors such as growth conditions are adjusted, needed for the growth for ensureing institute's cultivated animals.
Advantage for present invention：
The present invention prepares heterotrophic nitrification-aerobic denitrification activity cenobium by culture and forms flco, in low consumption carbon situation
Under, an ammonia nitrogen part in aquaculture system is changed by gained cenobium flco using limited organic carbon source by assimilation
Active cenobium flco organic nitrogen, while remaining most nitrogen pollutants are changed into nitrogen and are removed, realize cultivation water
Body original position aerobic nitrogen removal treatment and purification, specially：
1) present invention effectively overcomes the multiple technologies problem that traditional biological floc sedimentation cultural technique is faced.The present invention is built
Heterotrophic nitrification-aerobic denitrification functional activity cenobium flco, by heterotrophic nitrification-aerobic denitrification effect by most of nitrogen
Organic nitrogen is removed rather than be changed into, and then the consumption of organic carbon source is greatly reduced, organic carbon source can save 30%~50%, reduce
The addition of organic carbon source reduces cultivation water process and operation cost.
2) the characteristics of being rich in oxygen is directed in aquaculture water, and the present invention overcomes previous denitrifications to remove in advance
The problem for removing oxygen, it is oxygen-enriched in culturing pool without adding artificial deoxygenation and follow-up watercourse aeration technique, it is directly sharp
With heterotrophic nitrification-aerobic denitrification functional activity cenobium flco, nitrogen pollutant is removed by aerobic denitrification, is reduced
The complexity and operation energy consumption and cost of water treatment technology realize the aerobic removing in original position of nitrogen, ensure that aquaculture pond is normally supported
While growing, the simple operability of cultivation water in-situ treatment purification ensure that.
3) mixing foodstuff sterilized using aquiculture animal excrement residual bait, after reprocessing of the invention is as cultivated animals
Bait carries out cultivated animals feeding habits while strengthening input heterotrophic nitrification-aerobic denitrification bacterium into cultivating system
Domestication in advance improves the grazing rate that aquiculture animal generates culture in follow-up aquaculture pond cenobium flco, saves feed, real
The cycling and reutilization of bait is showed, ecological can solve excrement and residual bait retention problems in breeding water body with open arms, reduce environment
Pollution.
4) investment capital cost is cheap, can be according to actual conditions rapid build culturing pool in-situ purification system, system
Without changing water, it can effectively overcome the problems, such as that coastal region good water sources lack and cultivation Tail water reuse is overcome to pollute
Serious problem.
Specific embodiment
Below by embodiment, the present invention is further described, however the invention is not limited in following embodiments.
Embodiment 1
The advanced treating in situ of littoral zone prawn culturing base breeding water body purifies and animal in aquaculture pond is made normally to give birth to
It is long：
1) substances such as aquaculture pond excrement residual bait are collected, are placed in biological culture pond, Potassium Monopersulfate disinfectant powder is added, adds
Dosage is 0.5mg/L, and stirring makes it be uniformly mixed, and reacts 2h；By the wheat bran and corn of excrement residual bait and equal proportion after disinfection
The mixture of powder mixes, and adds composite bacteria, is placed in biological culture pond；Meanwhile aquaculture common antibiotics are added, into
Row antibiotic is tamed；The fatty acid ester compounded object of higher alcohols is added, plays defoaming and foam-inhibiting effect；It is slightly agitated for, controls in culture pond
Reaction system water content is in 40%~60% range, and C/N ratios are 25~35:In 1 range, pH is 6.5~7.5, culture 5~10
It；Microbial flocculant is added to biological culture pond, stirring makes it be uniformly mixed, and preparing has heterotrophic nitrification-aerobic denitrification
The microbial flocculation sediment of function.
Wherein, the additive amount of the excrement residual bait, wheat bran, corn flour and mixed bacteria is respectively that excrement residual bait additive amount is
70%, wheat bran and corn flour press 1:1 ratio mixing, total addition level 30%, composite bacteria additive amount are 0.02%；It is described different
Support nitrification-aerobic denitrifying bacteria, bacterium for producing flocculant of microbe composite bacteria be pseudomonad, Bacillus alcaligenes, Bacillus foecalis alkaligenes,
The strain that arthrobacterium, general foster sulphur coccus and rhodococcus erythropolis are mixed according to arbitrary proportion；The microbial flocculant is red flat red
The flocculant NOC-1, additive amount 10mg/L that coccus generates；The fatty acid ester compounded object of higher alcohols defoams for food industry
Agent, additive amount 1.0g/kg；The antibiotic of the cultivation system is tamed, and antibiotic used is sulphadiazine, terramycin, fluorobenzene
The mixture that Buddhist nun examines, Ofloxacin equal quality ratio mixes adds a concentration of 10 μ g/L.
2) zymotic fluid in step 1) the microbial flocculation sediment incubation is taken, it is addition amylodextrin, sucrose, low
The mixture of Fructooligosaccharides, additive amount 1.0% are uniformly sprayed at prawn feed surface, prepare heterotrophic nitrification-after mixing
Aerobic denitrifying bacteria sprays feed.
3) the microbial flocculation sediment for preparing step 1) presses 1 with the prawn feed after step 2) spraying:20 quality
Ratio is uniformly mixed, and is prepared prawn and is mixed foodstuff, feed mixing foodstuff into breeding water body at regular time and quantity daily, per day feeding volume
It is the 3%~10% of prawn weight, depending on the factors such as the size of prawn, survival rate, water quality, weather, feeding quality, ensures
Needed for the growth of prawn.
4) mixing foodstuff after 7~10 days is fed, it is (amylodextrin, sucrose, low that organic carbon source is properly added into breeding water body
The mixture of Fructooligosaccharides, wheat bran, corn flour equal quality ratio), the C/N ratios for adjusting water body are 10~20:1；Cultivation system is abundant
Aeration, maintenance water body dissolved oxygen amount are 3.0~9.0mg/L；Heterotrophic nitrification-aerobic denitrification bacterium, micro- life are added into breeding water body
Object bacterium for producing flocculant composite bacteria, composite bacteria inoculum concentration are 0.5 × 109cfu/m3, generate in final water body largely have it is different
Support the active cenobium flco of nitrification-aerobic denitrification function.
5) it is 10~20 persistently to adjust breeding water body C/N ratios:1, C/N deficiency adds carbon source, and monitors water quality index, control
Active a concentration of 10mL/L of cenobium flco, pH is in 7.8~8.2 ranges for control, and dissolved oxygen amount is 3.0~9.0mg/L, meets prawn
Growth demand, and then realize aquaculture water advanced treating in situ.When active cenobium concentration is higher, reduce organic carbon source and
Feed dosage when not proving effective still, filters extra active cenobium flco.
Breeding water body is tamed under the conditions of high nitrogen low-carbon, realizes the advanced treating in situ of aquaculture pond water body, removal
Organic matter, ammonia nitrogen, nitrite nitrogen and nitrate nitrogen.Wherein, it is 94% that TOC removal rates, which are 95%, TN removal rates, ammonia nitrogen removal
Rate is 93%, and nitrite removal rate is up to 96%.Water quality index is monitored, active a concentration of 10mL/L of cenobium flco is controlled, pH exists
In 7.8~8.2 ranges, C/N ratios are 10~20:In 1 range, dissolved oxygen amount is 3.0~9.0mg/L, meets the growth demand of prawn,
And then realize the advanced treating in situ of prawn culturing water body.
Embodiment 2
The advanced treating in situ of certain Tilapia mossambica aquaculture pond breeding water body purifies and makes animal normal growth in aquaculture pond：
1) substances such as aquaculture pond excrement residual bait are collected, are placed in biological culture pond, Potassium Monopersulfate disinfectant powder is added, adds
Dosage is 3.0mg/L, and stirring makes it be uniformly mixed, and reacts 8h；By the wheat bran and corn of excrement residual bait and equal proportion after disinfection
The mixture of powder mixes, and adds composite bacteria, is placed in biological culture pond；Meanwhile aquaculture common antibiotics are added, into
Row antibiotic is tamed；The fatty acid ester compounded object of higher alcohols is added, plays defoaming and foam-inhibiting effect；It is slightly agitated for, controls in culture pond
Reaction system water content is in 40%~60% range, and C/N ratios are 25~35:In 1 range, pH is 6.5~7.5, culture 5~10
It；Microbial flocculant is added to biological culture pond, stirring makes it be uniformly mixed, and preparing has heterotrophic nitrification-aerobic denitrification
The microbial flocculation sediment of function.
Wherein, the additive amount of the excrement residual bait, wheat bran, corn flour and mixed bacteria is respectively that excrement residual bait additive amount is
90%, wheat bran and corn flour press 1:1 ratio mixing, total addition level 10%, composite bacteria additive amount are 0.1%；It is described different
Support nitrification-aerobic denitrifying bacteria, bacterium for producing flocculant of microbe composite bacteria be pseudomonad, Bacillus alcaligenes, Bacillus foecalis alkaligenes,
The strain that arthrobacterium, general foster sulphur coccus and rhodococcus erythropolis are mixed according to arbitrary proportion；The microbial flocculant is red flat red
The flocculant NOC-1, additive amount 30mg/L that coccus generates；The fatty acid ester compounded object of higher alcohols defoams for food industry
Agent, additive amount 1.5g/kg；The antibiotic of the cultivation system is tamed, and antibiotic used is sulphadiazine, terramycin, fluorobenzene
The mixture that Buddhist nun examines, Ofloxacin equal quality ratio mixes adds a concentration of 20 μ g/L.
2) zymotic fluid in step 1) the bioflocculation sediment incubation is taken, it is addition amylodextrin, sucrose, oligomeric
The mixture of fructose, additive amount 3.0% are uniformly sprayed at tilapia feed surface, prepare heterotrophic nitrification-after mixing
Aerobic denitrifying bacteria sprays feed.
3) the bioflocculation sediment for preparing step 1) presses 1 with the tilapia feed after step 2) spraying:10 quality
Ratio is uniformly mixed, and is prepared Tilapia mossambica and is mixed foodstuff, feeds mixing foodstuff into breeding water body at regular time and quantity daily, feed daily
Amount is the 5%~10% of Tilapia mossambica weight, depending on the factors such as the size of Tilapia mossambica, survival rate, water quality, feeding quality, is protected
Needed for the growth for demonstrate,proving Tilapia mossambica.
4) mixing foodstuff after 7~10 days is fed, it is (amylodextrin, sucrose, low that organic carbon source is properly added into breeding water body
The mixture of Fructooligosaccharides, wheat bran, corn flour equal quality ratio), it is 10~20 to adjust water body C/N ratios:1；Cultivation system fully exposes
Gas, maintenance water body dissolved oxygen amount are 3.0~9.0mg/L；Heterotrophic nitrification-aerobic denitrification bacterium, microorganism are added into breeding water body
Bacterium for producing flocculant composite bacteria, composite bacteria inoculum concentration are 3.0 × 109cfu/m3, generating in final water body largely has heterotrophism
The active cenobium flco of nitrification-aerobic denitrification function.
5) it is 10~20 persistently to adjust breeding water body C/N ratios:1, C/N deficiency adds carbon source, and monitors water quality index, control
In 7.8~8.2 ranges, dissolved oxygen amount is 3.0~9.0mg/L, meets Tilapia mossambica by active a concentration of 30mL/L of cenobium flco, pH
Growth demand realizes the advanced treating in situ of aquaculture water.When active cenobium flco concentration is higher, organic carbon source and feeding are reduced
Expect dosage, when not proving effective still, filters extra active cenobium flco.
Breeding water body is tamed under the conditions of high nitrogen low-carbon, realizes the advanced treating in situ of aquaculture pond water body, removal
Organic matter, ammonia nitrogen, nitrite nitrogen and nitrate nitrogen.Wherein, it is 94% that TOC removal rates, which are 96%, TN removal rates, ammonia nitrogen removal
Rate is 96%, and nitrite removal rate is up to 99%.Water quality index is monitored, active a concentration of 30mL/L of cenobium flco is controlled, pH exists
In 7.8~8.2 ranges, C/N ratios are 10~20:In 1 range, dissolved oxygen amount is 3.0~9.0mg/L, meets the growth need of Tilapia mossambica
It asks, realizes the advanced treating in situ of Tilapia mossambica breeding water body.
Embodiment 3
The advanced treating in situ of certain shrimp ginseng shellfish aquaculture pond breeding water body purifies and makes animal normal growth in aquaculture pond：
1) substances such as aquaculture pond excrement residual bait are collected, are placed in biological culture pond, Potassium Monopersulfate disinfectant powder is added, adds
Dosage is 2.0mg/L, and stirring makes it be uniformly mixed, and reacts 5h；By the wheat bran and corn of excrement residual bait and equal proportion after disinfection
The mixture of powder mixes, and adds composite bacteria, is placed in biological culture pond；Meanwhile aquaculture common antibiotics are added, into
Row antibiotic is tamed；The fatty acid ester compounded object of higher alcohols is added, plays defoaming and foam-inhibiting effect；It is slightly agitated for, controls in culture pond
Reaction system water content is in 40%~60% range, and C/N ratios are 25~35:In 1 range, pH is 6.5~7.5, culture 5~10
It；Microbial flocculant is added to biological culture pond, stirring makes it be uniformly mixed, and preparing has heterotrophic nitrification-aerobic denitrification
The microbial flocculation sediment of function.
Wherein, the additive amount of the excrement residual bait, wheat bran, corn flour and mixed bacteria is respectively that excrement residual bait additive amount is
80%, wheat bran and corn flour press 1:1 ratio mixing, total addition level 20%, composite bacteria additive amount are 0.04%；It is described different
Support nitrification-aerobic denitrifying bacteria, bacterium for producing flocculant of microbe composite bacteria be pseudomonad, Bacillus alcaligenes, Bacillus foecalis alkaligenes,
The strain that arthrobacterium, general foster sulphur coccus and rhodococcus erythropolis are mixed according to arbitrary proportion；The microbial flocculant is red flat red
The flocculant NOC-1, additive amount 20mg/L that coccus generates；The fatty acid ester compounded object of higher alcohols defoams for food industry
Agent, additive amount 2.0g/kg；The antibiotic of the cultivation system is tamed, and antibiotic used is sulphadiazine, terramycin, fluorobenzene
The mixture that Buddhist nun examines, Ofloxacin equal quality ratio mixes adds a concentration of 15 μ g/L.
2) zymotic fluid in step 1) the bioflocculation sediment incubation is taken, it is addition amylodextrin, sucrose, oligomeric
The mixture of fructose, additive amount 2.0% are uniformly sprayed at shrimp ginseng shellfish feed surface, prepare heterotrophic nitrification-after mixing
Aerobic denitrifying bacteria sprays feed.
3) the bioflocculation sediment for preparing step 1) presses 1 with the shrimp ginseng shellfish feed after step 2) spraying:15 quality
Ratio is uniformly mixed, and is prepared mixing foodstuff, is fed mixing foodstuff into breeding water body at regular time and quantity daily, is shrimp per day feeding volume
Join the 3%~10% of shellfish weight, depending on joining the factors such as size, survival rate, water quality, weather, the feeding quality of shellfish according to shrimp, ensures
Shrimp is joined needed for the growth of shellfish.
4) mixing foodstuff after 7~10 days is fed, it is (amylodextrin, sucrose, low that organic carbon source is properly added into breeding water body
The mixture of Fructooligosaccharides, wheat bran, corn flour equal quality ratio), it is 10~20 to adjust water body C/N ratios:1；Cultivation system fully exposes
Gas, maintenance water body dissolved oxygen amount are 3.0~9.0mg/L；Heterotrophic nitrification-aerobic denitrification bacterium, microorganism are added into breeding water body
Bacterium for producing flocculant composite bacteria, composite bacteria inoculum concentration are 2.0 × 109cfu/m3, generating in final water body largely has heterotrophism
The active cenobium flco of nitrification-aerobic denitrification function.
5) it is 10~20 persistently to adjust breeding water body C/N ratios:1, C/N deficiency adds carbon source, and monitors water quality index, control
In 7.8~8.2 ranges, dissolved oxygen amount is 3.0~9.0mg/L by active a concentration of 20mL/L of cenobium flco, pH, meets shrimp ginseng shellfish
Growth demand, and then realize the advanced treating in situ of cultivation water.When active cenobium concentration is higher, reduces organic carbon source and feed is thrown
Dosage filters extra active cenobium flco.
Breeding water body is tamed under the conditions of high nitrogen low-carbon, realizes the advanced treating in situ of aquaculture pond water body, removal
Organic matter, ammonia nitrogen, nitrite nitrogen and nitrate nitrogen.Wherein, it is 96% that TOC removal rates, which are 96%, TN removal rates, ammonia nitrogen removal
Rate is 97%, and nitrite removal rate is up to 99%.Water quality index is monitored, active a concentration of 20mL/L of cenobium flco is controlled, pH exists
In 7.8~8.2 ranges, C/N ratios are 10~20:In 1 range, dissolved oxygen amount is 3.0~9.0mg/L, meets the growth need of shrimp ginseng shellfish
It asks, and then realizes the advanced treating in situ of breeding water body.
Embodiment 4
The advanced treating in situ of certain crucian cultivation base breeding water body purifies and makes animal normal growth in aquaculture pond：
1) substances such as aquaculture pond excrement residual bait are collected, are placed in biological culture pond, Potassium Monopersulfate disinfectant powder is added, adds
Dosage is 5.0mg/L, and stirring makes it be uniformly mixed, and reacts 12h；By the wheat bran and corn of excrement residual bait and equal proportion after disinfection
The mixture of powder mixes, and adds composite bacteria, is placed in biological culture pond；Meanwhile aquaculture common antibiotics are added, into
Row antibiotic is tamed；The fatty acid ester compounded object of higher alcohols is added, plays defoaming and foam-inhibiting effect；It is slightly agitated for, controls in culture pond
Reaction system water content is in 40%~60% range, and C/N ratios are 25~35:In 1 range, pH is 6.5~7.5, culture 5~10
It；Microbial flocculant is added to biological culture pond, stirring makes it be uniformly mixed, and preparing has heterotrophic nitrification-aerobic denitrification
The microbial flocculation sediment of function.
Wherein, the additive amount of the excrement residual bait, wheat bran, corn flour and mixed bacteria is respectively that excrement residual bait additive amount is
90%, wheat bran and corn flour press 1:1 ratio mixing, total addition level 10%, composite bacteria additive amount are 0.1%；It is described different
Support nitrification-aerobic denitrifying bacteria, bacterium for producing flocculant of microbe composite bacteria be pseudomonad, Bacillus alcaligenes, Bacillus foecalis alkaligenes,
The strain that arthrobacterium, general foster sulphur coccus and rhodococcus erythropolis mix in any proportion；The microbial flocculant is red flat red ball
The flocculant NOC-1, additive amount 50mg/L that bacterium generates；The fatty acid ester compounded object of higher alcohols defoams for food industry
Agent, additive amount 3.0g/kg；The antibiotic of the cultivation system is tamed, and antibiotic used is sulphadiazine, terramycin, fluorobenzene
Buddhist nun examines, the mixture of Ofloxacin equal quality ratio, adds a concentration of 30 μ g/L.
2) zymotic fluid in step 1) the bioflocculation sediment incubation is taken, it is addition amylodextrin, sucrose, oligomeric
The mixture of fructose, additive amount 5.0% are uniformly sprayed at crucian carp feed surface after mixing, it is good to prepare heterotrophic nitrification-
Oxygen denitrifying bacterium sprays feed.
3) crucian carp feed after the bioflocculation sediment for preparing step 1) is sprayed with step 2) is according to 1:10 ratio
It is uniformly mixed, prepares mixing foodstuff, feed mixing foodstuff into breeding water body at regular time and quantity daily, be crucian weight per day feeding volume
The 5%~10% of amount, depending on the factors such as the size of crucian, survival rate, water quality, feeding quality, ensures the growth institute of crucian
It needs.
4) mixing foodstuff after 7~10 days is fed, it is (amylodextrin, sucrose, low that organic carbon source is properly added into breeding water body
The mixture of Fructooligosaccharides, wheat bran, corn flour equal quality ratio), it is 10~20 to adjust water body C/N ratios:1；Cultivation system fully exposes
Gas, maintenance water body dissolved oxygen amount are 3.0~9.0mg/L；Heterotrophic nitrification-aerobic denitrification bacterium, microorganism are added into breeding water body
Bacterium for producing flocculant composite bacteria, composite bacteria inoculum concentration are 5.0 × 109cfu/m3, generating in final water body largely has heterotrophism
The active cenobium flco of nitrification-aerobic denitrification function.
5) it is 10~20 persistently to adjust breeding water body C/N ratios:1, C/N deficiency adds carbon source, and monitors water quality index, control
In 7.8~8.2 ranges, dissolved oxygen amount is 3.0~9.0mg/L, meets the life of crucian by active a concentration of 40mL/L of cenobium flco, pH
Long demand, and then realize the advanced treating in situ of cultivation water.When active cenobium concentration is higher, reduces organic carbon source and feed adds
Amount when not proving effective still, filters extra active cenobium flco.
Breeding water body is tamed under the conditions of high nitrogen low-carbon, realizes the advanced treating in situ of aquaculture pond water body, removal
Organic matter, ammonia nitrogen, nitrite nitrogen and nitrate nitrogen.Wherein, it is 98% that TOC removal rates, which are 98%, TN removal rates, ammonia nitrogen removal
Rate is 97%, and nitrite removal rate is up to 99%.Water quality index is monitored, active a concentration of 40mL/L of cenobium flco is controlled, pH exists
In 7.8~8.2 ranges, C/N ratios are 10~20:In 1 range, dissolved oxygen amount is 3.0~9.0mg/L, meets the growth demand of crucian,
And then realize the advanced treating in situ of crucian cultivation water body.
Claims (9)
1. a kind of method of aquiculture sewerage original position aerobic nitrogen removal treatment and purification, it is characterised in that：
1) it takes excrement residual bait in aquaculture pond to be mixed after processing with wheat bran and corn flour, composite bacteria, antibiotic, higher alcohols is added
Fatty acid ester compounded object, microbial flocculant, fermented and cultured, culture gained sediment are with heterotrophic nitrification-aerobic denitrification
The microbial flocculation sediment of function；Organic carbon source is added in culture gained zymotic fluid, aquiculture animal is sprayed at after mixing
Feed surface obtains heterotrophic nitrification-aerobic denitrification bacterium spraying feed；Gained microbial flocculation sediment is good with heterotrophic nitrification-
Edible for cultivation pool inner water production cultivated animals in pending aquaculture pond, culture 7 is added after the spraying feed mixing of oxygen denitrifying bacterium
~10 days；
2) it is 10~20 to adjust water body C/N ratios in aquaculture pond:1；Cultivation system is fully aerated, maintain water body dissolved oxygen amount be 3.0~
9.0mg/L adds composite bacteria into breeding water body, and then forms the heterotrophic nitrification-aerobic for capableing of purifying water body in water body
Denitrification function activity cenobium flco；Using above-mentioned acquisition heterotrophic nitrification-aerobic denitrification functional activity cenobium flco and
Heterotrophic nitrification-aerobic denitrification bacterium sprays feed and the microbial flocculation sediment with heterotrophic nitrification-aerobic denitrification function,
Realize the aerobic nitrogen removal advanced treating in situ of aquaculture pond water body, organics removal, ammonia nitrogen, nitrite nitrogen and nitrate nitrogen.
2. by the method for aquiculture sewerage original position aerobic nitrogen removal treatment and purification described in claim 1, it is characterised in that：It is described multiple
Combined bacteria kind is heterotrophic nitrification-aerobic denitrification bacterium and bacterium for producing flocculant of microbe, and step 1) composite bacteria additive amount is excrement
The 0.02%~0.20% of residual bait and wheat bran, corn flour mixture quality, step 2) composite bacteria inoculum concentration be 0.5 ×
109cfu/m3~5.0 × 109cfu/m3；
The heterotrophic nitrification-aerobic denitrification bacterium is pseudomonad, Bacillus alcaligenes, Bacillus foecalis alkaligenes, arthrobacterium, general foster sulphur coccus
In it is one or more；The bacterium for producing flocculant of microbe is pseudomonad, Bacillus alcaligenes, Bacillus foecalis alkaligenes, arthrobacterium, red
It is one or more in flat Rhodococcus sp.
3. by the method for aquiculture sewerage original position aerobic nitrogen removal treatment and purification described in claim 1, it is characterised in that：The step
It is rapid that excrement residual bait in aquaculture pond 1) is taken to mix to obtain mixture with wheat bran, corn flour after Potassium Monopersulfate sterilizes, mixing is added
The composite bacteria of amount of substance 0.02%~0.2%, addition antibiotic, the fatty acid ester compounded object of higher alcohols, mixing control after addition
Reaction system water content is that 40%~60%, C/N ratios are 25~35:1, pH is 6.5~7.5, fermented and cultured；Culture 5~10 days
Afterwards, microbial flocculant is added into culture, stirring makes it be uniformly mixed, and culture gained sediment is with heterotrophic nitrification-
The microbial flocculation sediment of aerobic denitrification function；Organic carbon source is added in culture gained zymotic fluid, water is sprayed at after mixing
Cultivated animals feed surface is produced, heterotrophic nitrification-aerobic denitrification bacterium spraying feed is obtained；By gained microbial flocculation sediment with it is different
It is added in pending aquaculture pond for cultivation pool inner water production cultivated animals food after supporting nitrification-aerobic denitrifying bacteria spraying feed mixing
With culture 7~10 days；Wherein, antibiotic addition makes final concentration of 10~100 μ g/L of antibiotic in mixture, higher alcohols fat
Acid esters compound additive amount is 1.0~3.0g/kg, and microbial flocculant additive amount is 10~100mg/L.
4. by the method for aquiculture sewerage original position aerobic nitrogen removal treatment and purification described in claim 3, it is characterised in that：The excrement
Just in percentage terms, 70%~90% excrement residual bait, 10%~30% wheat bran and corn flour are described for residual bait and wheat bran and corn flour
Wheat bran and maize flour press 1:1 mass ratio mixing.
5. by the method for aquiculture sewerage original position aerobic nitrogen removal treatment and purification described in claim 3, it is characterised in that：The hair
The organic carbon source of 1%~5wt% is added in zymotic fluid, is sprayed at aquiculture animal feed surface after mixing, it is good to obtain heterotrophic nitrification-
Oxygen denitrifying bacterium sprays feed；Wherein, organic carbon source is one or more in amylodextrin, sucrose, oligofructose.
6. by the method for aquiculture sewerage original position aerobic nitrogen removal treatment and purification described in claim 3, it is characterised in that：By gained
Microbial flocculation sediment with heterotrophic nitrification-aerobic denitrification function sprays feed with heterotrophic nitrification-aerobic denitrification bacterium
According to 1:10~20 mass ratio is uniformly mixed, and as aquiculture animal mixing foodstuff, is added in pending aquaculture pond,
Water body is adjusted while as aquatic animal feed.
7. by the method for aquiculture sewerage original position aerobic nitrogen removal treatment and purification described in claim 3, it is characterised in that：It is described micro-
Flocculating agent A J7002, the paecilomycerol that biological flocculant is the flocculant NOC-1 of rhodococcus erythropolis generation, soy sauce koji mould generates
It is one or more in the flocculant PF-101 of generation.
8. by the method for aquiculture sewerage original position aerobic nitrogen removal treatment and purification described in claim 1, it is characterised in that：The step
It is rapid that 2) into aquaculture pond, water body feeding machine carbon source adjusting C/N ratios are 10~20:1；The organic carbon source be amylodextrin, sucrose,
It is one or more in brown sugar, oligofructose, wheat bran, corn flour.
9. by the method for aquiculture sewerage original position aerobic nitrogen removal treatment and purification described in claim 1, it is characterised in that：The step
The rapid organic carbon source 2) persistently adjusted in breeding water body, and it is 10~20 to adjust pending breeding water body C/N ratios:1；Cultivation system
Fully aeration, maintenance water body dissolved oxygen amount are 3.0~9.0mg/L, and pH is 7.8~8.2, active cenobium flco a concentration of 10 in water body
~40mL/L utilizes above-mentioned gained heterotrophic nitrification-aerobic denitrification functional activity cenobium flco and the anti-nitre of heterotrophic nitrification-aerobic
Change bacterium spraying feed and the microbial flocculation sediment with heterotrophic nitrification-aerobic denitrification function, realizes aquaculture pond water body
Aerobic nitrogen removal advanced treating in situ, organics removal, ammonia nitrogen, nitrite nitrogen and nitrate nitrogen.
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