CN106754552A - A kind of method for cultivating biological flocculation and in all applications received in prawn culturing in riotous profusion - Google Patents
A kind of method for cultivating biological flocculation and in all applications received in prawn culturing in riotous profusion Download PDFInfo
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Abstract
A kind of method for cultivating biological flocculation and in all applications received in prawn culturing in riotous profusion, is related to the culture of biological flocculation.The culture biological flocculation includes bacillus pumilus, lactobacillus acidophilus and Pichia guilliermondii；Prepare bacillus pumilus agent, lactobacillus acidophilus agent and Pichia guilliermondii bacterium bacterium powder；Daily by the way of every mu of water splashing bacillus bacterium powder, saccharomycete bacterium powder and lactic acid bacteria agent, and lower floor sets nanometer aeration pipe aeration in culturing pool surrounding, forms floc sedimentation；C/N ratios to breeding water body are adjusted, and C/N is in (12~14) ︰ 1 for control；Biological flocculation cultivate after the agent of daily every mu of water splashing bacillus pumilus, Pichia guilliermondii bacterium bacterium powder and lactobacillus acidophilus agent during cultivating.The biological flocculation of culture apply in prawn culturing in riotous profusion in all being received.
Description
Technical field
The present invention relates to the culture of biological flocculation, more particularly, to a kind of method for cultivating biological flocculation and it is all receive it is in riotous profusion right
Application in shrimp aquaculture.
Background technology
Biological flocculation technological core is by adding carbon source and probiotics in breeding water body, improving water body C/N ratios, promote
Heterotrophic microorganism purifies water and flocculation to play.Microorganism is to remaining bait and debirs in traditional cultivating system
Conversion ratio be extremely low, only 5%~7% nitrogen and 4%~6% phosphorus have regulated and controled breeding water body by microorganism conversion
Carbon and nitrogen, microorganism will significantly improve to the conversion ratio of residual bait excrement, if adding carbon in breeding water body according to C/N=8-10
Source, cultivating system organic nitrogen and inorganic nitrogen are available, and ammonia can be consumed only, and concentration is in 5h for the ammoniacal nitrogen of 10mg/L
Can all remove.Research has shown that, by adding carbon source namely using biological flocculation technology, can effectively remove having for breeding water body
Machine nitrogen.Confirmed by many experiments, carbon, nitrogen quantity ratio are the major influence factors to heterotroph breeding in water body, thus are carried
Go out the technology that application C/N ratios regulate and control heterotrophic microorganism, it is believed that when only C/N ratios reach more than 10, be just conducive to heterotroph
Development.
Biological flocculation technology is fully being aerated using addition carbon source control water body C/N ratios, the heterotrophic microorganism in culturing pool
Under the conditions of form biological flocculation, both can as cultivation crop extra food source, again can by heterotrophic microorganism absorb turn
Change residual bait, cultivation crop excreta and harmful pathogen and its metabolin can effectively be degraded.By the control of microorganism
Easy produced problem during high-density breeding is solved with management, life is formed by specific bacillus and lactic acid bacteria etc.
Thing floc sedimentation, the floc sedimentation is attached microbial extracellular products extracellular polymer body, polysaccharide, more by with zoogloea, trichobacteria as core
Quadrafos etc., and divalence cation, the heterotroph of agglomeration, nitrifier, denitrifying bacteria, microalgae, fungi, protozoan
Deng the biological floc sedimentation for being formed.The magnitude range of floc sedimentation is 800 μm or so, and specific surface area is 50~150cm2/ml.Work in floc sedimentation
Organism account for 10%~90%, therefore it has self-reproduction ability.Floccule based on bacterium can be as bait
Source, there is provided part nutrition, the nutrition that supplement mixed feed is not enough, so as to improve utilization of the prawn to feed protein indirectly
Rate.Both the accumulation of the harmful substance in breeding water body had been reduced, efficiency of feed utilization had been improved but also as food, so as to improve cultivation
Yield, increases cultivation income.
A kind of the applicant's probiotics and preparation method and application disclosed in Chinese patent CN103497906A,
It is related to aquaculture.Probiotics contain at least one and Lactobacillus plantarum in bacillus subtilis, bacillus pumilus,
At least one in lactobacillus acidophilus；
Bacillus pumilus includes：Bacillus pumilus (Bacillus pumilus) 1A08151, deposit number CCTCC
NO:M 2013291；
Lactobacillus acidophilus includes：Lactobacillus acidophilus (Lactobacillus acidophilus) PL7, deposit number CCTCC
NO:M 2013294。
Promote prawn or grouper growth preparing, improve prawn or grouper immunity and premunition, controlling disease, carry
High viability, applies in purifying aquaculture water quality preparation.
The applicant discloses one kind and can degrade carbon chain lengths for 12C~36C straight chains in Chinese patent CN102242071A
Novel microorganism --- the Pichia guilliermondii of alkane, strain number 510-6jm (Pichia guilliermondii
sp.510-6jm)CCTCC NO：M 2010173.A kind of novel species Pichia guilliermondii from deep-sea, is degraded using the bacterium
Hydrocarbon, is mainly used in biological prosthetic and industry cleaning link industry.The invention discloses the raw material needed for cultivating the bacterium, profit
Substantial amounts of somatic cells can be cultivated for decomposing petroleum hydrocarbon with the raw material.
The content of the invention
It is an object of the invention to provide a kind of method for cultivating biological flocculation and in all applications received in prawn culturing in riotous profusion.
The culture biological flocculation includes：Bacillus pumilus (Bacillus pumilus) 1A08151, lactobacillus acidophilus
(Lactobacillus acidophilus) PL7 and Pichia guilliermondii (Pichia guilliermondii sp.510-
6jm)510-6jm；
The deposit number of bacillus pumilus (Bacillus pumilus) 1A08151 is CCTCC NO:M
2013291；
The deposit number of lactobacillus acidophilus (Lactobacillus acidophilus) PL7 is CCTCC NO:M
2013294；
The deposit number of Pichia guilliermondii (the Pichia guilliermondii sp.510-6jm) 510-6jm
It is CCTCC NO：M20100173.
The method of the culture biological flocculation is comprised the following steps；
1) bacillus pumilus agent, lactobacillus acidophilus agent and Pichia guilliermondii bacterium bacterium powder are prepared, specific method is as follows：
(1) actication of culture that will be frozen accordingly lines corresponding flat board, then the monoclonal after activation is inoculated in into kind
Sub- medium culture, prepares seed culture medium 500ml, sterilizing；
(2) fermentation medium is prepared, using 50L fermentation mediums as primary-seed medium, lactobacillus-fermented is added low
It is a kind of as lactobacter growth agent is promoted in Fructooligosaccharides or trehalose etc., sterilize；
(3) the primary activation seed that culture is obtained is cultivated in being inoculated in 50L primary-seed mediums, is inoculated with by culture transferring pipe
Cultivated in 1000L fermentation mediums；
(4) after fermentation ends, bacillus pumilus and Pichia guilliermondii bacterium are collected by centrifugation bacterium by tube centrifuge
Body；
(5) in bacterium mud addition vitamin C, trehalose, amylodextrin, FOS for obtaining step (4) etc. at least
Two kinds, as protective agent, are played harmonious protection effect, after mixing, bacterium mud are laid in the sample panel of sample freeze dryer, and by bacterium
Mud is divided into fritter；
(6) after lactobacillus-fermented terminates, dilute with water adds vitamin C, trehalose, amylodextrin, FOS etc.
In at least two as protective agent；
2) daily by the way of every mu of water splashing bacillus bacterium powder, saccharomycete bacterium powder and lactic acid bacteria agent, and
Lower floor sets nanometer aeration pipe aeration in culturing pool surrounding, forms floc sedimentation；
3) the C/N ratios to breeding water body are adjusted, and C/N is in (12~14) ︰ 1 for control；
4) after biological flocculation is cultivated during cultivating the agent of daily every mu of water splashing bacillus pumilus, also cover complete red season
Saccharomycete bacterium powder and lactobacillus acidophilus agent.
In step 1) in (1st) part, the culture can quiescent culture 18h；The sterilizing can be in 115 DEG C of sterilizing 30min.
In step 1) in (2nd) part, the addition of the FOS or trehalose can be by mass percentage lactic acid
The 0.5% of bacterium, the sterilizing can be in 115 DEG C of sterilizing 30min.
In step 1) in (3rd) part, it is described be inoculated in 50L primary-seed mediums in culture can quiescent culture 12h；Institute
Stating culture in being inoculated in 1000L fermentation mediums can cultivate 48h.
In step 1) in (5th) part, the vitamin C, trehalose, amylodextrin, the addition of FOS press matter
Amount percentage can be the 1% of bacterium mud.
In step 1) in (6th) part, the dilute with water can in equal volume be diluted using running water, then adjust pH to
3~4；The vitamin C, trehalose, amylodextrin, the addition of FOS can be by mass percentage lactobacillus-fermented knot
The 1% of product after beam.
In step 2) in, the amount of splashing of the bacillus bacterium powder and saccharomycete bacterium powder can be 200~300g, the lactic acid
The amount of splashing of bacteria agent can be 1~2L；The aeration can be operated continuously 3 days, and the diameter of the floc sedimentation for being formed can be 800~1000
μm。
In step 3) in, the regulation can be adjusted using the molasses after dilution to the C/N ratios of breeding water body.
In step 4) in, the amount of splashing of the bacillus pumilus agent and Pichia guilliermondii bacterium bacterium powder can for 80~
150g, the amount of splashing of lactobacillus acidophilus agent can be 0.5~1L.
Bacillus pumilus vitality is extremely strong, to strong environmental adaptability, be difficult variation, extracellular enzyme is more, physiological metabolism
Product is nontoxic and bacillus product, is suitable for industrialization large-scale production, and technology maturation, production stabilization, yield is high, the cycle
It is short.Especially the hypopus acid and alkali-resistance of brood cell, drought-resistant high temperature can be stored for a long time.There is height in animal intestinal tract sour environment
That spends stablizes qualitative, can make gut pH and ammonia density reduction, can produce more strongly active protease and amylase, promotes aquatic products
The digestion of animal, and the immunity of animal can be improved, suppress part pathogen, therefore with the work(of certain disease preventing and treating
Energy.The strain physiological metabolism is vigorous, can rapidly be bred in water body, and produces substantial amounts of extracellular enzyme, available, consumption pond
In residual bait and the macromolecular organic matter such as animal excrements.Larger molecular organicses are broken down into the organic acid of small molecule, extracellular domain amino
The materials such as interior product.
The unicellular eukaryote that Pichia guilliermondii bacterium is separated from the natural environment of ocean, thalline is rich in albumen
Matter, glycogen unrighted acid, young somatropin, astaxanthin etc., can significantly improve the survival rate of the aquatic livestock young, change
Kind adult feeding effect, and aquatic livestock immunity can be strengthened, antibiotic dosage is reduced, it is the excellent additive of ecologic breeding.
The biological flocculation of present invention culture apply in prawn culturing in riotous profusion in all being received.Specifically by bacillus powder, ferment
Female bacterium powder end and 3 kinds of mix preparations of lactic acid bacterial liquid are splashed the application in aquaculture, and promoting water body to form biological flocculation has
Beneficial to culturing economic crop-Environment of Litopenaeus vannamei Low enhance immunity and premunition, suppression by the microbial disease of arc, increase food ration
Improve the application in all breeding survival rates.
The present invention can within a short period of time form biological flocculation and can maintain life by Reasonable Regulation And Control during cultivating
The stable existence of thing floc sedimentation, reduces water body ammonia nitrogen and nitrite, especially increases prawn food ration, promotes Environment of Litopenaeus vannamei Low life
It is long, immunity of prawn and premunition are improved, improve prawn survival rate.
The present invention controls water with continuation, few additive addition microbial germ powder and liquid bacterial agent with the molasses after dilution
Body carbon-nitrogen ratio is (lower floor is fully aerated and to form low speed circulation and be conducive to the quick shape of biological flocculation in the scopes of 12~14) ︰ 1, culturing pool
Into.
The strain characteristic and function that the present invention is selected are as follows
Bacillus pumilus (Bacillus pumilus) 1A08151, deposit number CCTCC NO：M2013291, brood cell
Bacillus vitality is extremely strong, to strong environmental adaptability, be difficult variation, extracellular enzyme is more, physiological metabolism product is nontoxic and brood cell's bar
Bacterium product.
Pichia guilliermondii (Pichia guilliermondii sp.510-6jm) 510-6jm deposit numbers CCTCC
NO：The unicellular eukaryote that M20100173 is separated from the natural environment of ocean, thalline is rich in protein, unsaturated lipid
Fat acid, young somatropin, astaxanthin etc., can significantly improve the survival rate of the aquatic livestock young, improve adult feeding effect,
And aquatic livestock immunity can be strengthened, antibiotic dosage is reduced, it is the excellent additive of ecologic breeding
Lactobacillus acidophilus (Lactobacillus acidophilus) PL7, deposit number CCTCC NO：M2013294, can
To enter aquatic livestock enteron aisle, can be strong and long in enteron aisle time to live with regulating intestinal canal colony balance, acid producing ability, suppress cause of disease
Bacteria growing, improves cultivated animals immunity of organisms and premunition.
Above-mentioned 3 kinds of microbial cells are preserved in China typical culture collection center on June 25th, 2013, in preservation
Heart location is China, Wuhan, Wuhan University, postcode 430072.
Utilization bacillus of the present invention, saccharomycete, lactic acid bacteria are aerated by nanotube, control water body carbon-nitrogen ratio
A kind of method of fast culture biological flocculation, in all applications received in prawn culturing in riotous profusion, can splash described three kinds through sieving
The life of larger floc sedimentation amount is quickly formed in three days after being selected in cultured prawn aspect there is the compound micro-ecological preparation of positive effect
Thing floc sedimentation, prawn premunition and immunity are improved during prawn culturing in riotous profusion in all receiving, and purifying aquaculture water quality increases prawn and ingests
Amount, improving the aspects such as cultivation quality and yield has remarkable result.
Specific embodiment
It is prepared by the microbial preparation thallus that embodiment 1 is used to cultivate biological flocculation
Lactobacillus acidophilus (Lactobacillus acidophilus) PL7 deposit number CCTCC NO：The training of M2013294
Support：Starting strain is pressed the order culture of inclined-plane, liquid seeds, liquid fermentation, inclined-plane and seed culture medium are routine MRS cultures
Base；Lactobacillus-fermented adds 0.5% FOS as promotion lactobacter growth agent, fermentative medium formula (W/V)：Molasses
6%th, brown sugar 3%, yeast extract 0.03%, peptone 0.6%, oligosaccharide 0.5%, magnesium sulfate 0.05%, manganese sulfate 0.05%, carbon
Sour calcium 0.5%；Fermentation condition is 37 DEG C of 36~48h of quiescent culture, is diluted 2 times for the treatment of after fermentation ends, adjust pH to 3~
4, and trehalose, vitamin C are added, FOS, mannitol, at least one in amylodextrin, and in 1%~3% ratio
Well mixed, bacterium amount is more than 1,000,000,000/mL after measured.
Bacillus (Bacillus pumilus) 1A08151, deposit number CCTCC NO：The culture side of M2013291
Method：Starting strain is pressed the order culture of inclined-plane, liquid seeds, large volume liquid fermentation, inclined-plane and seed culture medium are conventional
Nutrient agar or broth bouillon；The culture medium prescription of large volume liquid fermentation：W/V：Beancake powder 3%, starch
1.5%th, corn pulp 1%, glucose 0.5%, (NH4)2SO40.1%th, NH4CL 0.1%, KH2PO40.05%th, NaH2PO4
0.5%th, MgSO40.05%th, MnSO40.05%；Fermentation culture conditions are 28~37 DEG C of ventilation 36~48h of culture, fermentation ends
Be collected by centrifugation thalline, at least two in vitamin C, trehalose, amylodextrin, the FOS of addition 1% as protective agent,
Harmonious protection effect is played, carries out well mixed lyophilized, according to formulation requirement, addition solid adjuvant material is made in tablet, pulvis
It is a kind of；The solid adjuvant material includes at least one in lime stone, zeolite powder, activated carbon, diatomite and adds corresponding micro
Element.Bacterium amount is more than 4,000,000,000/g after measured.
Pichia yeast (Pichia guilliermondii sp.510-6jm) 510-6jm deposit number CCTCC NO：
The cultural method of M20100173：Starting strain is pressed the order culture of inclined-plane, liquid seeds, secondary seed and liquid fermentation；Tiltedly
Face activating solid culture medium is：YPD solid mediums；Liquid seed culture medium is inoculated in for YPD fluid nutrient medium Shaking cultures
Secondary seed and large volume liquid fermentation medium culture medium：Molasses 6%, glucose 2%, yeast extract 0.5%, peptone 2%,
KH2PO40.05%th, MgSO40.05%th, individually sterilize (NH4)2SO40.1%；Fermentation ends are collected by centrifugation thalline, addition 1%
Vitamin C, trehalose, amylodextrin, FOS at least two as protective agent, play harmonious protection effect, enter
Row is well mixed lyophilized, and according to formulation requirement, addition solid adjuvant material is made the one kind in tablet, pulvis；The solid adjuvant material bag
Include at least one in agstone, zeolite powder, activated carbon, diatomite and add corresponding trace element.Bacterium amount is big after measured
In 4,000,000,000/g.
The experiment of the fast culture biological flocculation of embodiment 2
First, materials and methods
1. test products：The compound micro-ecological preparation of embodiment 1.
2. water body is tested：Test water takes from sand filtration seawater.
3. test site：Experiment is carried out in the glass fibre cylinder of 80cm × 100cm × 100cm (length × width × height).Examination
Test under cylinder natural conditions near the window disposed within and place, and arranged at same direction nanometer aeration pipe in glass fibre cylinder surrounding, point
Cylinder bottom 10CM and 50CM are not placed in highly.
4. test method：Use 6 mouthfuls of specifications for：In the glass fibre cylinder of 80cm × 100cm × 100cm (length × width × height)
The cultivation body all tails of prawn in riotous profusion 100 of receiving of a length of 3cm are tested, wherein 3 mouthfuls is experimental group, 3 mouthfuls is control group；It is 30 days cycles, real
Testing group takes molasses to adjust water body C/N than control in (12~14) ︰ 1；Microorganism formulation (bacillus 1g, lactobacillus preparation
6mL, saccharomycete 2g) mix with maize cob meal 5g, water content is no more than 60%, and 37 DEG C of fermentations are overnight splashed afterwards；Control group is only
Carry out the nursing that normally feeds intake.Statistics biological flocculation deposition：The deposition of biological flocculation is determined using precipitation funnel, sampling stands
Reading after 15min.
Determine ammonia nitrogen, content of nitrite in water body：Water sample is carried out by 0.2 μm of miillpore filter using vacuum filtration pump
Filtering, filtrate for water quality index determine, ammonia nitrogen (NH3-N) assay using hypobromite oxidation method (National Bureau of Oceanography,
2007), cultured water (NO2--N) content uses hydrochloride naphthodiamide spectrophotometry (National Bureau of Oceanography, 1991), nitre
Sour nitrogen (NO3--N) assay uses Zn-Cd reduction (National Bureau of Oceanography, 1991).Statistics shrimp body survival number and body weight,
Body is long.
First, result of the test
1st, the influence that biological flocculation is formed
Experimental group determined the deposition of biological flocculation, sampling by precipitating funnel at the 3rd day of addition microorganism formulation
Reading after 15min is stood, floc sedimentation amount basically reaches 12ml/L, and several days floc sedimentations afterwards are stable in 10ml/L or so, compare
More fast and stable turns out floc sedimentation on the method time of other method culture biological flocculation；Control group is surveyed by precipitating funnel
The deposition for determining biological flocculation is only 3ml/L or so.
2nd, to water body ammonia nitrogen, the influence of nitrite
The content of water body ammonia nitrogen, nitrite in experiment starts to determine 6 mouthfuls of test cylinder (jar)s on the 7th day：3 mouthfuls of water bodies of experimental group
Middle ammonia nitrogen, content of nitrite are below 0.01mg/L, and ammonia-nitrogen content is in 0.01~0.02mg/L, nitrous in control group water body
Phosphate content is in 0.02~0.03mg/L；
3rd, the influence obtained to survival rate, shrimp body body long
At the 30th day of experiment, the survival shrimp in 6 mouthfuls of cylinders and its body body long must be counted；Experimental group Average Survival 93
Tail prawn, a length of 5.5cm of average body, weight average are 2.48g, and the tail prawn of control group Average Survival 78, average body are a length of
4.5cm, weight average are 1.36g
Application test 1 of the fast culture biological flocculation of the invention of embodiment 3 in prawn culturing
1st, test products：The compound micro-ecological preparation of embodiment 1.
2nd, prawn is tested：The prawn whole process that test period throws seedling to 30 days is used.If 5 mouthfuls of experiment pools and 5 mouthfuls of control ponds, body
Product is 5m × 5m × 1.5m, and the cement pit of depth of water 1.2m, the surrounding configuration row of nanometer aeration pipe two, height is respectively away from bottom of pond 10cm
With 60cm.
3rd, test site：ZhangZhou Dongshan Island prawn culturing.
4th, test method：During daily raising, the composite bacteria agent of Example 1 is splashed in culturing pool, daily usage and dosage：Gemma
Bacillus and saccharomycete 50g, lactic acid bacteria liquid 200mL and 200g maize cob meal mixed fermentation, 37 DEG C of fermentations are overnight splashed；And add
Molasses control water body carbon-nitrogen ratio after dilution is in (each two h of 12~14) ︰ 1, morning, noon and afternoon unlatching aeration tubes.Control group is only carried out normally
Feed intake nursing.
5th, result of the test：
The using effect of the composite bacteria agent of embodiment 1 is mainly reflected in quick formation biological flocculation, and experimental group is in experiment the 3rd
Its floc sedimentation amount is basically reached 10ml/L and maintains water body by can periodically be stablized with control water body carbon-nitrogen ratio using composite bacteria agent
Middle floc sedimentation content accounts for water body volume 10% or so, specially：(1) water pH value stabilization, floc sedimentation amount is more and stable；(2) periodically
Using the composite bacteria agent of embodiment 1, water body ammonia nitrogen, nitrite maintain reduced levels, and breeding process does not use disinfectant substantially,
The growth of shrimp body is fast, and period is high without there is disease, survival rate；(3) food ration is increased, gut of shrimp increases slightly, and food is full of in enteron aisle,
Vigor is higher；Cultivation is whole, and experimental group has no that disease occurs substantially using 4 mouthfuls of experiment pool prawns of the composite bacteria agent of embodiment 1, and
Often there is disease in 4 mouthfuls of control ponds, using more disinfectant, wherein pond is compareed in having 1 mouthful seriously causes the big rule of prawn because of disease
Mould is dead and finally arrange the pool.
Application test 2 of the fast culture biological flocculation of the invention of embodiment 4 in prawn culturing
1st, test products：The compound micro-ecological preparation and its floc sedimentation cultural method of embodiment 1.
2nd, prawn is tested：Environment of Litopenaeus vannamei Low.
3rd, factory site is tested：To the feed coefficient in shrimp seedling cultivation factory, area is one mu of every pond, water in Changle of Fujian Province city plum blossom town
Deep 3m.
4th, test method：Four mouthfuls of shrimp culture ponds of selection, wherein being flatly control pond, three mouthfuls is experiment pool, cultivates early stage
Daily usage and dosage：Bacillus and saccharomycete 1000g, lactic acid bacteria liquid 10L and 12kg maize cob meal mixed fermentation, 32~37
Splashed after DEG C fermentation 36h, splash daily and respectively open h of nanometer aeration pipe two once and in the morning, noon and afternoon (nanometer aeration pipe is arranged
For：Culturing pool surrounding same direction, is highly away from two height of bottom of pond 20cm, 150cm), probiotics after floc sedimentation culture well
The amount of splashing halves.Experiment pool water body adds the molasses for diluting and supplies floc sedimentation growth of microorganism demand as carbon source simultaneously.Feed intake
Feed identical with control pond.
5th, experimental result：
The using effect of the composite bacteria agent of embodiment 1 is mainly reflected in quick formation biological flocculation, and experimental group is in experiment the 3rd
Its floc sedimentation amount basically reach 8ml/L and by can periodically be stablized using composite bacteria agent and control water body carbon-nitrogen ratio and maintain water body in
Floc sedimentation content accounts for water body volume 12% or so, specially：(1) water pH value stabilization, floc sedimentation amount is more and stable；(2) periodically make
With the composite bacteria agent of embodiment 1, water body ammonia nitrogen, nitrite maintain reduced levels, and breeding process does not use disinfectant, shrimp substantially
Body growth is fast, and period is high without there is disease, survival rate；(3) food ration is increased, gut of shrimp increases slightly, and food is full of in enteron aisle；
(4) three mouthfuls of experimental group survival rates are above control group 25% or so, are higher than control group about 800~1000kg per pond yield.
The invention provides the method and the method that is applied to aquaculture of a kind of fast culture biological flocculation, by toward water
Body addition microbial bacterial agent, with commercially available maize cob meal as floc sedimentation carrier and bottom of pond in lower floor's same direction fully exposed using nanotube
Gas forms circulation, and adds molasses dilution control water body carbon-nitrogen ratio and promote biological flocculation to be formed, and remains biological during cultivating
Floc sedimentation accounts for the volume ratio of breeding water body 10% or so, and the present invention is used as carbon source by adding microbial bacterial agent, molasses dilution
Reduce biological flocculation toxigenic capacity, promote biological flocculation to be more quickly generated, obtain the biology that particle diameter is 800~1000 μm
Floc sedimentation；Fully aeration can effectively facilitate water body flow and increase dissolved oxygen amount, be conducive to bottom of pond material to float and realize biological flocculation
Settling flux and recycling；The physics and chemistry such as all ammonia nitrogen, nitrite, vibrios received in prawn high-density breeding water body in riotous profusion are effectively controlled to join
Number, improves efficiency of feed utilization, adjusts breeding water body water quality, reduces the incidence of disease.
Claims (10)
1. it is a kind of cultivate biological flocculation method, it is characterised in that it is comprised the following steps；
1) bacillus pumilus agent, lactobacillus acidophilus agent and Pichia guilliermondii bacterium bacterium powder are prepared；
The bacillus pumilus is bacillus pumilus (Bacillus pumilus) 1A08151, and the lactobacillus acidophilus is
Lactobacillus acidophilus (Lactobacillus acidophilus) PL7, the Pichia guilliermondii bacterium is Pichia guilliermondii
(Pichia guilliermondii sp.510-6jm)510-6jm；
The deposit number of bacillus pumilus (Bacillus pumilus) 1A08151 is CCTCC NO:M 2013291；
The deposit number of lactobacillus acidophilus (Lactobacillus acidophilus) PL7 is CCTCC NO:M
2013294；
The deposit number of Pichia guilliermondii (the Pichia guilliermondii sp.510-6jm) 510-6jm is
CCTCC NO：M20100173；
2) daily by the way of every mu of water splashing bacillus bacterium powder, saccharomycete bacterium powder and lactic acid bacteria agent, and in cultivation
Lower floor sets nanometer aeration pipe aeration in the surrounding of pond, forms floc sedimentation；
3) the C/N ratios to breeding water body are adjusted, and C/N is in (12~14) ︰ 1 for control；
4) after biological flocculation is cultivated during cultivating the agent of daily every mu of water splashing bacillus pumilus, Pichia guilliermondii
Bacterium bacterium powder and lactobacillus acidophilus agent.
2. as claimed in claim 1 it is a kind of cultivate biological flocculation method, it is characterised in that in step 1) in, it is described prepare it is short and small
The specific method of bacillus agent, lactobacillus acidophilus agent and Pichia guilliermondii bacterium bacterium powder is as follows：
(1) actication of culture that will be frozen accordingly lines corresponding flat board, then the monoclonal after activation is inoculated in into seed training
Base culture is supported, seed culture medium 500ml, sterilizing is prepared；
(2) fermentation medium is prepared, using 50L fermentation mediums as primary-seed medium, lactobacillus-fermented adds oligomeric fruit
It is a kind of as lactobacter growth agent is promoted in sugar or trehalose etc., sterilize；
(3) the primary activation seed that culture is obtained is cultivated in being inoculated in 50L primary-seed mediums, is inoculated in by culture transferring pipe
Cultivated in 1000L fermentation mediums；
(4) after fermentation ends, bacillus pumilus and Pichia guilliermondii bacterium are collected by centrifugation thalline by tube centrifuge；
(5) in bacterium mud addition vitamin C, trehalose, amylodextrin, FOS for obtaining step (4) etc. at least two
As protective agent, harmonious protection effect is played, after mixing, bacterium mud is laid in the sample panel of sample freeze dryer, and bacterium mud is divided
Into fritter；
(6) after lactobacillus-fermented terminates, dilute with water, in adding vitamin C, trehalose, amylodextrin, FOS etc.
At least two used as protective agent.
3. a kind of method for cultivating biological flocculation as claimed in claim 2, it is characterised in that in step 1) in (1st) part, institute
It is quiescent culture 18h to state culture；The sterilizing is the 30min that sterilized at 115 DEG C；
In step 1) in (2nd) part, the addition of the FOS or trehalose is by mass percentage lactic acid bacteria
0.5%, the sterilizing is the 30min that sterilized at 115 DEG C.
4. a kind of method for cultivating biological flocculation as claimed in claim 2, it is characterised in that in step 1) in (3rd) part, institute
It is quiescent culture 12h to state culture in being inoculated in 50L primary-seed mediums；It is described to be inoculated in culture in 1000L fermentation mediums
It is culture 48h.
5. a kind of method for cultivating biological flocculation as claimed in claim 2, it is characterised in that in step 1) in (5th) part, institute
It is by mass percentage the 1% of bacterium mud to state vitamin C, trehalose, amylodextrin, the addition of FOS.
6. a kind of method for cultivating biological flocculation as claimed in claim 2, it is characterised in that in step 1) in (6th) part, institute
Stating dilute with water is diluted in equal volume using running water, then adjusts pH to 3~4；The vitamin C, trehalose, gelatinized corn starch
Essence, FOS addition by mass percentage for lactobacillus-fermented terminate rear product 1%.
7. as claimed in claim 1 it is a kind of cultivate biological flocculation method, it is characterised in that in step 2) in, the bacillus
The amount of splashing of bacterium powder and saccharomycete bacterium powder is 200~300g, and the amount of splashing of the lactic acid bacteria agent is 1~2L；The aeration is
Continuous operation 3 days, a diameter of 800 μm of the floc sedimentation for being formed.
8. as claimed in claim 1 it is a kind of cultivate biological flocculation method, it is characterised in that in step 3) in, the regulation is to adopt
The C/N ratios of breeding water body are adjusted with the molasses after dilution.
9. as claimed in claim 1 it is a kind of cultivate biological flocculation method, it is characterised in that in step 4) in, the short and small gemma
The amount of splashing of bacillus agent and Pichia guilliermondii bacterium bacterium powder is 80~150g, and the amount of splashing of lactobacillus acidophilus agent is 0.5~1L.
10. the biological flocculation such as claim 1~9 methods described culture apply in prawn culturing in riotous profusion in all being received.
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| CN107365731A (en) * | 2017-09-26 | 2017-11-21 | 郑州蓝海渔业有限公司 | A kind of biological flocculation strain formula suitable for culture of Penaeus vannamei |
| CN108179157A (en) * | 2017-12-28 | 2018-06-19 | 广州日日红水环境处理有限公司 | A kind of production technology of denitrogenation-type biological flocculation |
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| CN111410290A (en) * | 2019-06-24 | 2020-07-14 | 上海海洋大学 | Nitrification construction and water body reuse method for biological floc aquaculture denitrification wastewater |
| CN110628644A (en) * | 2019-09-09 | 2019-12-31 | 天津农学院 | Novel biological floc, application thereof and method for marking crude litopenaeus vannamei by using same |
| CN110628644B (en) * | 2019-09-09 | 2021-12-31 | 天津农学院 | Novel biological floc, application thereof and method for marking crude litopenaeus vannamei by using same |
| CN110663601A (en) * | 2019-09-27 | 2020-01-10 | 华南师范大学 | Method for controlling vibrio quantity in prawn culture system |
| CN110663601B (en) * | 2019-09-27 | 2022-07-29 | 华南师范大学 | Method for controlling vibrio quantity in prawn culture system |
| CN110800888A (en) * | 2019-11-14 | 2020-02-18 | 岳阳渔美康生物科技有限公司 | Composition for culturing plankton, preparation method and application thereof |
| CN112075368A (en) * | 2020-09-23 | 2020-12-15 | 江苏宝源生物科技有限公司 | Prawn digestive tract thickening method |
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