CN109430579A - EM bacterium calcium and magnesium compound micro-ecological preparation and preparation method thereof and application method - Google Patents
EM bacterium calcium and magnesium compound micro-ecological preparation and preparation method thereof and application method Download PDFInfo
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Abstract
The invention discloses a kind of EM bacterium calcium and magnesium compound micro-ecological preparation and preparation method thereof and application methods.It include the EM compound bacteria being solidificated on zeolite powder, vitamin, glucose, calcium lactate, microelement in said preparation.Preparation method includes: that EM strain and light salt brine or culture solution is taken to pour into ferment tank in proportion, and EM compound bacteria is made；The zeolite powder of 90-98%, the EM compound bacteria combination drying of 2-10% are taken, cured EM compound bacteria is made；Take cured EM compound bacteria 40-60%, vitamin 5-15%, glucose 15-25%, calcium lactate 10-20%, other microelement 3-10% to be mixed evenly to obtain the final product.The probiotics can efficient-decomposition pond organic pollutant, improve the absorption of the aquatic crustacean to calcium, magnesium, phosphorus such as shrimp crab, shrimp crab crust quickly promoted to harden；It can be obviously improved shrimp crab quality, promote the weight gain of shrimp crab, effectively improve cultivation survival rate.
Description
Technical field
The invention belongs to microorganisms technical field, be related to a kind of EM bacterium calcium and magnesium compound micro-ecological preparation and preparation method thereof and
Application method.
Background technique
With the fast development of culture fishery, cultivation scale is increasing, since germplasm is degenerated, pond aging, feed
The improper reasons such as feed cause disease frequently to occur, and disease problem has become at present restricts one of the development of China's culture fishery
Key factor.For disease preventing and treating, the dosage of antibiotic is continued to increase, and is largely produced not using drug to ecological environment
Good influence, or even threaten to the health care belt of the mankind.Carry out Non-environmental Pollution Aquiculture to have become a top priority.
Probiotics are also named probiotics, refer under microecology theories guidance using known beneficial microbe, through training
The biologic live bacteria preparation of animal is used for made of the special process such as feeding, rejuvenation, fermentation, embedding, drying.Since microorganism formulation can
By adjusting the intracorporal microecological balance of cultivation water, purifies water, reach and improve the breed variety general level of the health and improvement cultivation ring
The purpose in border, and microorganism formulation has that noresidue, the side effects such as have no drug resistance, is pollution-free, and it to a certain extent can portion
Divide substitution or complete substitute antibiotics, is created condition for the production of nuisanceless aquatic products.
It is the advantages that microorganism formulation is with its nonhazardous, noresidue, more next with the popularization and promotion of Non-environmental Pollution Aquiculture
More by the favor of vast aquaculture production person, meanwhile, it is also more next using the benefit of microorganism formulation in aquaculture production
It is more significant.Microorganism formulation correctly and is reasonably used, the economic benefit of aquaculturist is remarkably improved.Currently, being used for
The main bacteria seed of the microorganism formulation of aquaculture has photosynthetic bacteria, saccharomycete, lactic acid bacteria, nitrobacteria, bacillus etc., with
Based on compound product, such as EM bacterium.EM bacterium will be screened beneficial to micro- using ratio appropriate and unique zymotechnique
Biology mixed culture, the complex microbial community of formation.EM bacterium is compound by probiotics such as photosynthetic bacteria, lactic acid bacteria, saccharomycete
It cultivates.Beneficial microbe in EM bacterium makes the organic substance in water through a series of decomposition such as fixed nitrogen, photosynthetic, synthesis
Various nutrients are formed, for the growth and breeding of itself and food organisms, while increasing the dissolved oxygen in water, reduce ammonia, vulcanization
The content of the noxious materials such as hydrogen, reaches purpose of purifying the water quality.
Summary of the invention
It is an object of the present invention to overcome the deficiencies of the prior art and provide a kind of EM bacterium calcium and magnesium compound micro-ecological preparation and
Preparation method and application method.
The purpose of the present invention is what is be achieved through the following technical solutions:
A kind of EM bacterium calcium and magnesium compound micro-ecological preparation of the present invention, it is included the following components'mass percentage:
Wherein, the cured EM compound bacteria is the EM compound bacteria being solidificated on zeolite powder.
Further, the cured EM compound bacteria is to mix the EM compound bacteria of the zeolite powder of 90-98% and 2-10%
It closes, is dry, manufactured cured EM compound bacteria.
Further, the EM bacterium calcium and magnesium compound micro-ecological preparation, includes the following components'mass percentage:
Further, in the EM compound bacteria, containing bacillus megaterium, Rhodopseudomonas palustris, lactobacillus acidophilus,
Saccharomyces cerevisiae, unwrapping wire aspergillus, wherein mass content >=60% of Rhodopseudomonas palustris；Total bacteria concentration in the EM compound bacteria
Greater than 30 × 108cfu/ml。
Further, the vitamin is one of C, vitamin D, vitamin A, vitamin B, vitamin E or several
Kind.
Further, the microelements of calcium added in the probiotics, preferably calcium lactate may be used also in addition to calcium lactate
Add calcium citrate or calcium gluconate.The other microelements added in the probiotics further include zinc, magnesium, iron, copper,
Manganese, iodine, selenium, cobalt etc., the substance specifically added are zinc gluconate, magnesium citrate, ferrous sulfate (or frerrous chloride), copper sulphate
One or more of Deng.
A kind of preparation method of EM bacterium calcium and magnesium compound micro-ecological preparation of the present invention, includes the following steps:
(1), prepared by EM compound bacteria: the ratio of EM strain and light salt brine or culture solution 1:5-15 by volume being taken to pour into fermentation
Tank fermentation, keeps temperature 25-30 degree, and it is spare that EM compound bacteria is made in anaerobic fermentation culture 4-10 days；
(2), EM compound bacteria (first step manufactured goods) mixing of the zeolite powder, 2-10% of 90-98% is taken, it is dry, it is made solid
The EM compound bacteria of change is spare；(effect for adding zeolite powder is the composite bacteria for solidifying liquid)；
(3), take cured EM compound bacteria (second step manufactured goods) 40-60%, vitamin 5-15%, glucose 15-25%,
Calcium lactate 10-20%, other microelement 3-10% mixing, stir；
(4), it crushes, is packed after the screen to filtrate.
Further, the EM compound bacteria in the step (1) is prepared as follows to obtain:
(A) preparation of culture solution
Sal-ammoniac (NH4Cl): sodium bicarbonate (NaHCO3): dipotassium hydrogen phosphate (K2HPO4): sodium acetate (CH3COONa): chlorine
Change magnesium (MgCl2): sodium chloride (NaCl): glucose (C6H12O6): distilled water (H2O)=15:20:10:35:8:40:200:
19672, it sufficiently dissolves, it is spare that culture solution is made；
(B) preparation of EM compound bacteria
The Rhodopseudomonas palustris in photosynthetic bacteria is chosen, strain content is 30 × 108Cfu/ml or more；In lactic acid bacteria
Lactobacillus acidophilus, strain content be 5 × 108Cfu/ml or more；Saccharomyces cerevisiae in saccharomycete, strain content be 20 ×
108Cfu/ml or more；Unwrapping wire aspergillus strain content in filamentous fungi is 5 × 106Cfu/ml or more；It chooses from high yield shrimp
The phosphate-solubilizing bacteria bacillus megaterium extracted in crab sediment of pond, strain content are 60 × 108Cfu/ml or more；Take 8-12 parts
Rhodopseudomonas palustris, 4-6 parts of lactobacillus acidophilus, 1-3 parts of brewer's yeasts, 1-2 parts of unwrapping wire aspergillus, 1-3 parts of huge gemma bars
Bacterium pours into fermentor with the ratio of the 1:5-15 by volume of culture solution made from step (A), is kept for 25-30 DEG C of temperature, anaerobism
Fermented and cultured 4-10 days, EM compound bacteria is made.
Mechanism of the invention is as follows: zeolite is a kind of aqueous aluminosilicate natural crystal with tetrahedral structure, right
NH3 isopolarity molecule and microorganism have very strong adsorption capacity, can using zeolite to the good adsorption characteristic of microorganism in water body
Using natural zeolite as the carrier of attached growth of microorganisms, EM compound bacteria is solidificated on zeolite powder, it is compound to may advantageously facilitate EM
The performance of the effects of decomposition of bacterium, synthesis, meanwhile, pass through addition vitamin, glucose, calcium lactate, other microelements, supplement
Promote the nutriment of aquatic livestock growth, further promotes the absorption of nutriment.
The characteristics of EM bacterium calcium and magnesium compound micro-ecological preparation product of the invention is: phosphorus decomposing replenishes the calcium, the growth promoting that shells, Replacement Battalion
It supports, increase yield.
(1) organic calcium, magnesium are processed by ionization and used to this product, reaches excellent activity state, degree of hydrolysis is high, easily
It is absorbed and is metabolized by shrimp crab；
(2) the shelling growth of the aquatic crustacean such as shrimp crab is rapidly promoted；
(3) active organic calcium, magnesium, phosphorus and various microelements and mineral needed for supplementing and maintaining the growth of shrimp crab crust
Matter.
The physicochemical character of this product: pale yellow powder
The main component of this product: calcium lactate, glucose, vitamin, microelement, EM compound bacteria etc..
The active constituent content of this product: every gram contains probiotics 2 × 108It is a.
The use scope of this product: being suitable for all sea water and fresh water aquatic product animal raise seedlings pond and growing pond, is especially suitable for cultivation
The old pond of many years and high density shrimp crab cultivating pool.
The application method of this product: full pond is uniformly splashed after being diluted with 10-20 times of water or mixed feed is fed.
This product is based on mix fodder is for oral administration, supplemented by water body of splashing.
The dosage of this product:
(1) it splashes water body, regulating water quality: the water surface (1 meter of the depth of water) uses 50-100 grams of this product per acre, splashes one every 7-10 days
It is secondary.
(2) mixed feed feeds use: every 50 kilogram of feed adds 200-300 grams of this product.
(3) nursery: before seedling enters pond, per cubic meter of water is splashed 2-5 grams, after seedling enters pond, splashes one every 2-3 days
Secondary, dosage per cubic meter of water is splashed 1-3 grams.
The application notice of this product:
1, as far as possible without using the chemicals and antibiotic medicine for having bactericidal effect in three days before and after this product of splashing；
2, it is not influenced by weather, water temperature using this product, commonly using can ensure that water quality is good, human body is healthy.
Beneficial effects of the present invention:
EM bacterium calcium and magnesium compound micro-ecological preparation product of the invention has the advantages that compared with prior art
1), this product is according to shrimp crab physiology, growth characteristic and shrimp and crab ponds water quality characteristics, using modern biological project skill
The environmental type composite microbial bacterium product of art exploitation.
2), this product be select the phosphate-solubilizing bacteria (bacillus megaterium) that is extracted from high yield shrimp and crab ponds bed mud with it is other
EM bacterium is compound, through domestication, Culturing, using liquid or solid state fermentation culture, and compounds organic calcium, magnesium and various trace elements
Manufactured compound micro-ecological preparation.
3), this product is launched to after water body, and phosphate solubilizing bacteria can convert shrimp crab for the invalid phosphorus in sediment of pond and organic matter
Etc. the available available phosphorus of aquatic crustacean, to overcome the phosphorus of high density shrimp crab cultivating pool to limit, meanwhile, composite microbial bacterium
Can also efficient-decomposition pond organic pollutant, improve the absorption of the aquatic crustacean to calcium, magnesium, phosphonium ion such as shrimp crab.
4), this product can quickly promote shrimp crab crust harden, prevent all kinds of shrimp crab soft shells because of caused by mineral deficiency,
The symptoms such as shell unsuccessful.Shrimp crab quality can be obviously improved commonly using this product, promote the metabolism of shrimp crab and vivo protein
Synthesis promotes the weight gain of shrimp crab.Shrimp crab immunity of organisms also can be improved, enhance premunition and anti-stress ability, improve the shelling of shrimp crab
Synchronism, avoid cutting one another's throat, effectively improve cultivation survival rate.
5), this product can also be used in other aquaculture of aquatic animal, play supplement water body calcium, magnesium, phosphorus and other microelements,
The effects of cleaning is with stablizing breeding water body, improving water quality.
Specific embodiment
The present invention is further illustrated with reference to embodiments.
Embodiment 1
A kind of EM bacterium calcium and magnesium compound micro-ecological preparation of the present invention, it is included the following components'mass percentage:
Wherein, the cured EM compound bacteria is the EM compound bacteria being solidificated on zeolite powder, it is by 95% zeolite powder
It is mixed with 5% EM compound bacteria, is dry, manufactured cured EM compound bacteria.
Wherein, in the EM compound bacteria, contain bacillus megaterium, Rhodopseudomonas palustris, lactobacillus acidophilus, wine brewing
Yeast, unwrapping wire aspergillus, wherein mass content >=60% of Rhodopseudomonas palustris；Total bacteria concentration in the EM compound bacteria is greater than
30×108cfu/ml.The bacillus megaterium is the phosphate-solubilizing bacteria extracted from high yield shrimp and crab ponds bed mud.
Wherein, other microelements are zinc gluconate and magnesium citrate.
A kind of preparation method of EM bacterium calcium and magnesium compound micro-ecological preparation of the present invention, includes the following steps:
(1), prepared by EM compound bacteria:
EM compound bacteria in the step is prepared as follows to obtain:
(A) preparation of culture solution
Sal-ammoniac (NH4Cl): sodium bicarbonate (NaHCO3): dipotassium hydrogen phosphate (K2HPO4): sodium acetate (CH3COONa): chlorine
Change magnesium (MgCl2): sodium chloride (NaCl): glucose (C6H12O6): distilled water (H2O)=15:20:10:35:8:40:200:
19672, it sufficiently dissolves, it is spare that culture solution is made；
(B) preparation of EM compound bacteria
The Rhodopseudomonas palustris in photosynthetic bacteria is chosen, strain content is 30 × 108cfu/ml or more；In lactic acid bacteria
Lactobacillus acidophilus, strain content is 5 × 108cfu/ml or more；Saccharomyces cerevisiae in saccharomycete, strain content be 20 ×
108cfu/ml or more；Unwrapping wire aspergillus strain content in filamentous fungi is 5 × 106cfu/ml or more；It chooses from high yield shrimp
The phosphate-solubilizing bacteria bacillus megaterium extracted in crab sediment of pond, strain content are 60 × 108cfu/ml or more；Take 8-12 parts
Rhodopseudomonas palustris, 4-6 parts of lactobacillus acidophilus, 1-3 parts of brewer's yeasts, 1-2 parts of unwrapping wire aspergillus, 1-3 parts of huge gemma bars
Bacterium, with culture solution made from step (A), the ratio of 1:10 pours into ferment tank by volume, is kept for 28 degree of temperature, anaerobism hair
Ferment culture 8 days, it is spare that EM compound bacteria is made；
(2), the EM compound bacteria (first step manufactured goods) of 95% zeolite powder+5% is taken to mix, it is dry, cured EM is made
Compound bacteria is spare；
(3), cured EM compound bacteria (second step manufactured goods) 50%, vitamin C 10%, glucose 20%, calcium lactate are taken
20%, other microelements 5% mix, and stir；
(4), it is crushed after stirring, is packed after the screen to filtrate.
Embodiment 2
A kind of EM bacterium calcium and magnesium compound micro-ecological preparation of the present invention, it is included the following components'mass percentage:
Wherein, the cured EM compound bacteria is the EM compound bacteria being solidificated on zeolite powder, it is by 98% zeolite powder
It is mixed with 2% EM compound bacteria, is dry, manufactured cured EM compound bacteria.
Wherein, in the EM compound bacteria, contain bacillus megaterium, Rhodopseudomonas palustris, lactobacillus acidophilus, wine brewing
Yeast, unwrapping wire aspergillus, wherein mass content >=60% of Rhodopseudomonas palustris；Total bacteria concentration in the EM compound bacteria is greater than
30×108cfu/ml.The bacillus megaterium is the phosphate-solubilizing bacteria extracted from high yield shrimp and crab ponds bed mud.
Wherein, other microelements are zinc gluconate, magnesium citrate, frerrous chloride and copper sulphate.
A kind of preparation method of EM bacterium calcium and magnesium compound micro-ecological preparation of the present invention, includes the following steps:
(1), prepared by EM compound bacteria:
EM compound bacteria in the step is prepared as follows to obtain:
(A) preparation of culture solution
Sal-ammoniac (NH4Cl): sodium bicarbonate (NaHCO3): dipotassium hydrogen phosphate (K2HPO4): sodium acetate (CH3COONa): chlorine
Change magnesium (MgCl2): sodium chloride (NaCl): glucose (C6H12O6): distilled water (H2O)=15:20:10:35:8:40:200:
19672, it sufficiently dissolves, it is spare that culture solution is made；
(B) preparation of EM compound bacteria
The Rhodopseudomonas palustris in photosynthetic bacteria is chosen, strain content is 30 × 108cfu/ml or more；In lactic acid bacteria
Lactobacillus acidophilus, strain content is 5 × 108cfu/ml or more；Saccharomyces cerevisiae in saccharomycete, strain content be 20 ×
108cfu/ml or more；Unwrapping wire aspergillus strain content in filamentous fungi is 5 × 106cfu/ml or more；It chooses from high yield shrimp
The phosphate-solubilizing bacteria bacillus megaterium extracted in crab sediment of pond, strain content are 60 × 108cfu/ml or more；Take 8-12 parts
Rhodopseudomonas palustris, 4-6 parts of lactobacillus acidophilus, 1-3 parts of brewer's yeasts, 1-2 parts of unwrapping wire aspergillus, 1-3 parts of huge gemma bars
Bacterium, and culture solution made from step (A) take the ratio of EM strain and culture solution 1:15 by volume to pour into ferment tank, protect
30 degree of temperature are held, anaerobic fermentation culture 4 days, it is spare that EM compound bacteria is made；
(2), the EM compound bacteria (first step manufactured goods) of 98% zeolite powder+2% is taken to mix, it is dry, cured EM is made
Compound bacteria is spare；
(3), cured EM compound bacteria (second step manufactured goods) 60%, vitamin C+vitamin D 12%, glucose are taken
15%, calcium lactate 10%, other microelements 3% mix, and stir；
(4), it is crushed after stirring, is packed after the screen to filtrate.
Embodiment 3
A kind of EM bacterium calcium and magnesium compound micro-ecological preparation of the present invention, it is included the following components'mass percentage:
Wherein, the cured EM compound bacteria is the EM compound bacteria being solidificated on zeolite powder, it is by 90% zeolite powder
It is mixed with 10% EM compound bacteria, is dry, manufactured cured EM compound bacteria.
Wherein, in the EM compound bacteria, contain bacillus megaterium, Rhodopseudomonas palustris, lactobacillus acidophilus, wine brewing
Yeast, unwrapping wire aspergillus, wherein mass content >=60% of Rhodopseudomonas palustris；Total bacteria concentration in the EM compound bacteria is greater than
30×108cfu/ml.The bacillus megaterium is the phosphate-solubilizing bacteria extracted from high yield shrimp and crab ponds bed mud.
Wherein, other microelements are zinc gluconate, magnesium citrate, ferrous sulfate and copper sulphate.
A kind of preparation method of EM bacterium calcium and magnesium compound micro-ecological preparation of the present invention, includes the following steps:
(1), prepared by EM compound bacteria:
The Rhodopseudomonas palustris in photosynthetic bacteria is chosen, strain content is 30 × 108cfu/ml or more；In lactic acid bacteria
Lactobacillus acidophilus, strain content is 5 × 108cfu/ml or more；Saccharomyces cerevisiae in saccharomycete, strain content be 20 ×
108cfu/ml or more；Unwrapping wire aspergillus strain content in filamentous fungi is 5 × 106cfu/ml or more；It chooses from high yield shrimp
The phosphate-solubilizing bacteria bacillus megaterium extracted in crab sediment of pond, strain content are 60 × 108cfu/ml or more；Take 8-12 parts
Rhodopseudomonas palustris, 4-6 parts of lactobacillus acidophilus, 1-3 parts of brewer's yeasts, 1-2 parts of unwrapping wire aspergillus, 1-3 parts of huge gemma bars
Bacterium pours into ferment tank with the ratio of light salt brine 1:5 by volume, is kept for 25 degree of temperature, anaerobic fermentation culture 10 days, be made
EM compound bacteria is spare；
(2), the EM compound bacteria (first step manufactured goods) of 90% zeolite powder+10% is taken to mix, it is dry, cured EM is made
Compound bacteria is spare；
(3), cured EM compound bacteria (second step manufactured goods) 40%, vitamin C 5%, glucose 25%, calcium lactate are taken
20%, other microelements 10% mix, and stir；
(4), it is crushed after stirring, is packed after the screen to filtrate.
Application Example:
By EM bacterium calcium and magnesium compound micro-ecological preparation product (the hereinafter referred to as excellent bacterium of calcium phosphorus) of the invention, it is applied to high density shrimp
Crab cultivating pool.
The application method of EM bacterium calcium and magnesium compound micro-ecological preparation product (the excellent bacterium of calcium phosphorus) of the invention are as follows: mix fodder, which takes orally, is
It is main, supplemented by water body of splashing；Specifically used method are as follows:
Shrimp crab nursery stage: this product is used for nursery；Before seedling enters pond, per cubic meter of water is splashed 2 grams；After seedling enters pond,
It spilled once every 2-3 days, dosage is that per cubic meter of water is splashed 1 gram.
The shrimp crab growth stage: being used for mixed feed for this product and feed, and every 50 kilogram of feed adds 250 grams of this product；
Meanwhile it being used for regulating water quality, full pond is uniformly splashed after being diluted with 10-20 times of water, and the water surface (1 meter of the depth of water) sprinkles per acre
50-100 grams of this product is spilt, spilled once every 7-10 days.
Application implementation case 1:
Water body: the river crab pool of two adjacent 15 mu of waters surface is handled, mode is fed using intensive culture, equal 1.2 meters of the depth of water or so,
Water colour is light green, and juvenile crab eats food normal without death condition, is divided into experimental group and control group, and the observation period is April 1 to July 15
Day.
It putting situation and pond situation in a suitable place to breed: entrusting one's child to the care of sb. pond and put 160 jin of seedling, 110/jin entrust one's child to the care of sb. 5 mu of pond, mainly plant waterweed,
It puts pond in a suitable place to breed and adds and entrust one's child to the care of sb. 15 mu of pond, mainly plant hydrilla verticillata, 10000 tail of seedling is mentioned after 2 shells.
Case study: influence of the calcium substance to river crab specification is supplemented during river crab shell.
Processing scheme:
Experimental group: mixed feed uses 250 grams of excellent bacterium of calcium phosphorus of the invention using every 50 kilogram of feed.It is dilute with 10 times of waters
Rear full pool spilling head is released, the water surface (1 meter of the depth of water) uses 100 grams of the excellent bacterium of calcium phosphorus per acre, spilled once every 7 days.
Control group: without using the excellent bacterium of calcium phosphorus of the invention.
Other rearing conditions and feeding volume are all the same.
Tracking result after processing:
The analysis of cases:
1. calcium, phosphorus are elements necessary to river crab grows, the calcium phosphorus content of crab body is not with addition different calcium phosphorus in bait
Amount and it is significantly different, but in water add calcium after crab body calcium and phosphorus content can improve.This illustrates that addition calcium is improving river in water
While crab is to the utilization efficiency of calcium source, also improve river crab to the utilization rate of bait phosphorus, be conducive to body maintain to calcium, phosphorus and
The balance and normal physiological function of inorganic elements.The content of calcium phosphorus directly affects the double rate of river crab shell and survives in water body
Rate, therefore the weight gain of river crab can be promoted, promote the synthesis of river crab metabolism and vivo protein, river crab quality can be obviously improved.
2. ionized calcium and calcium gluconate containing high-content in the excellent bacterium of calcium phosphorus of the invention can be dissolved in water entirely, benefit is absorbed
It is high with rate.The excellent bacterium weight of calcium phosphorus is 500 grams, and wherein the active calcium ion content is 202.5 grams in water.
3. not lacking phosphorus in river crab pond, phosphorus content is very high in opposite soil, but available available phosphorus is very
It is low, because the phosphorus in soil is mostly insoluble chemical compound.The excellent bacterium of calcium phosphorus of the invention is launched to after water body, and phosphate solubilizing bacteria can be by pond
Invalid phosphorus in bed mud and organic matter is converted into the absorbable available phosphorus utilized of river crab, to overcome the phosphorus in high-density breeding pond to limit
System, and remaining organophosphorus pesticide in water body of degrading, to play the role of removing toxic substances.In addition, selenate and vitamin can be with
The constitution of enhancing river crab improves anti-stress ability of cultivated animals well, smooth when making its weak constitution in shell and environmental catastrophe
It crosses difficult barriers, improves survival rate.
Application implementation case 2:
Handle water body: shrimp mode, the equal 1.2 meters of left sides of the depth of water are supported using rice field in the cray pool of two adjacent 5 mu of waters surface
The right side, shrimp seedling release time are 25 days 2 months, and injected volume is 8000 tails/mu, puts 100 tails of seedling specification/jin, is divided into experimental group and control
Group, process cycle are 25 days.
Experimental group: every 50 kilogram of feed uses 250 grams of excellent bacterium of calcium phosphorus, and before seedling enters pond, per cubic meter of water is splashed calcium phosphorus
2 grams of excellent bacterium after seedling enters pond, spilled once every 3 days, 1 gram of the excellent bacterium of the calcium phosphorus that dosage per cubic meter of water is splashed of the invention.
Control group: without using the excellent bacterium of calcium phosphorus of the invention.
During experiment, control group and the daily dusk 17:00 of experimental group are fed once, other rearing conditions are all the same.Into
After row cultivation 25 days, starts the commercial sized prawn that fishing meets listing specification, measure the yield, specification and quality of control group and experimental group
(see the table below):
Control group shrimp body quality is summarized:
1. green shell shrimp and red shell shrimp accounting are similar, there is soft shell, toughness is general；
2. shrimp body surface is obscure, bottom plate majority is not clean, and incomplete limbs are less, and appearance is not damaged and bacterium infection and disease disease
Shape；
3. constitution is preferable, vigor is general, meets water energy and quickly escapes；
4. shrimp enteron aisle is virtually free from disconnected section, even thickness, the symptoms such as no jejunum, dilute dung.
Experimental group shrimp body quality is summarized:
1. being mostly red shell shrimp, green shell shrimp accounting is less, and shell is hard, and toughness is preferable；
2. shrimp body surface is bright, bottom plate is clean, and without incomplete limbs, appearance is not damaged and bacterium infection and disease symptoms；
3. it is physical strong, it is energetic, it meets water and quickly escapes, being placed on water plant voluntarily quickly to climb into water；
4. shrimp enteron aisle is virtually free from disconnected section, even thickness, the symptoms such as no jejunum, dilute dung.
The analysis of cases:
Contain a large amount of calcium ions and magnesium ions and other microelements in the excellent bacterium of calcium phosphorus of the invention, can effectively supplement cray and slough off
Nutriment required for during shell can greatly reduce the unsuccessful phenomenon of shell, increase cray husking yield and shorten shelling
New shell firm time afterwards to improve survival rate, and improves the quality of commercial sized prawn.
Claims (10)
1. a kind of EM bacterium calcium and magnesium compound micro-ecological preparation, which is characterized in that it is included the following components'mass percentage:
Wherein, the cured EM compound bacteria is the EM compound bacteria being solidificated on zeolite powder.
2. EM bacterium calcium and magnesium compound micro-ecological preparation as described in claim 1, which is characterized in that
The cured EM compound bacteria is to mix the zeolite powder of 90-98% with the EM compound bacteria of 2-10%, is dry, manufactured
Cured EM compound bacteria.
3. EM bacterium calcium and magnesium compound micro-ecological preparation as claimed in claim 1 or 2, which is characterized in that the EM bacterium calcium and magnesium is multiple
Probiotics are closed, are included the following components'mass percentage:
4. EM bacterium calcium and magnesium compound micro-ecological preparation as claimed in claim 1 or 2, which is characterized in that the EM compound bacteria,
In contain bacillus megaterium, Rhodopseudomonas palustris, lactobacillus acidophilus, saccharomyces cerevisiae, unwrapping wire aspergillus, wherein the red vacation in marsh
Mass content >=60% of monad；Total bacteria concentration in the EM compound bacteria is greater than 30 × 108cfu/ml.
5. EM bacterium calcium and magnesium compound micro-ecological preparation as claimed in claim 1 or 2, which is characterized in that the vitamin is dimension
One or more of raw element C, vitamin D, vitamin A, vitamin B, vitamin E.
6. EM bacterium calcium and magnesium compound micro-ecological preparation as claimed in claim 1 or 2, which is characterized in that other micro members
Element is one or more of zinc gluconate, magnesium citrate, ferrous sulfate, copper sulphate.
7. a kind of preparation method of EM bacterium calcium and magnesium compound micro-ecological preparation as claimed in claim 1 or 2, which is characterized in that packet
Include following step:
(1), prepared by EM compound bacteria: taking the ratio of EM strain and light salt brine or culture solution 1:5-15 by volume to pour into fermentor and sends out
Ferment, keeps temperature 25-30 degree, and it is spare that EM compound bacteria is made in anaerobic fermentation culture 4-10 days；
(2), the EM compound bacteria mixing of the zeolite powder, 2-10% of 90-98% is taken, it is dry, it is spare that cured EM compound bacteria is made；
(3), cured EM compound bacteria 40-60%, vitamin 5-15%, glucose 15-25%, calcium lactate 10-20%, other is taken
Microelement 3-10% mixing, stirs；
(4), it crushes, is packed after the screen to filtrate.
8. the preparation method of EM bacterium calcium and magnesium compound micro-ecological preparation as claimed in claim 7, which is characterized in that including following steps
It is rapid:
(1), prepared by EM compound bacteria: taking the ratio of EM strain and light salt brine or culture solution 1:10 by volume to pour into fermentor and sends out
Ferment, keeps temperature 25-30 degree, and it is spare that EM compound bacteria is made in anaerobic fermentation culture 4-10 days；
(2), it takes the EM compound bacteria of 95% zeolite powder+5% to mix, is dry, it is spare that cured EM compound bacteria is made；
(3), cured EM compound bacteria 50%, vitamin C 10%, glucose 20%, calcium lactate 15%, other microelements are taken
5% mixing, stirs；
(4), it crushes, is packed after the screen to filtrate.
9. the preparation method of EM bacterium calcium and magnesium compound micro-ecological preparation as claimed in claim 7 or 8, which is characterized in that the step
Suddenly the EM compound bacteria in (1) is prepared as follows to obtain:
(A) preparation of culture solution
Sal-ammoniac (NH4Cl): sodium bicarbonate (NaHCO3): dipotassium hydrogen phosphate (K2HPO4): sodium acetate (CH3COONa): magnesium chloride
(MgCl2): sodium chloride (NaCl): glucose (C6H12O6): distilled water (H2O)=15:20:10:35:8:40:200:19672,
It is spare that culture solution is made in sufficiently dissolution；
(B) preparation of EM compound bacteria
The Rhodopseudomonas palustris in photosynthetic bacteria is chosen, strain content is 30 × 108cfu/ml or more；It is thermophilic in lactic acid bacteria
Lactobacillus lactis, strain content are 5 × 108cfu/ml or more；Saccharomyces cerevisiae in saccharomycete, strain content be 20 ×
108cfu/ml or more；Unwrapping wire aspergillus strain content in filamentous fungi is 5 × 106cfu/ml or more；It chooses from high yield shrimp
The phosphate-solubilizing bacteria bacillus megaterium extracted in crab sediment of pond, strain content are 60 × 108cfu/ml or more；Take 8-12 parts
Rhodopseudomonas palustris, 4-6 parts of lactobacillus acidophilus, 1-3 parts of brewer's yeasts, 1-2 parts of unwrapping wire aspergillus, 1-3 parts of huge gemma bars
Bacterium pours into fermentor with the ratio of the 1:5-15 by volume of culture solution made from step (A), is kept for 25-30 DEG C of temperature, anaerobism
Fermented and cultured 4-10 days, EM compound bacteria is made.
10. a kind of application method of the EM bacterium calcium and magnesium compound micro-ecological preparation as described in claim 1-6 is any, feature exist
In full pond is uniformly splashed after being diluted with 10-20 times of water or mixed feed is fed；
The aquatic product animal raise seedling phase: the probiotics are used for nursery；Before seedling enters pond, per cubic meter of water is splashed 2-5 grams；Children
It after seedling enters pond, spilled once every 2-3 days, dosage is that per cubic meter of water is splashed 1-3 grams.
The aquatic livestock growth stage: being used for mixed feed for the probiotics and feed, and every 50 kilogram of feed adds 200-300 grams should
Probiotics；
For regulating water quality: full pond is uniformly splashed after being diluted with 10-20 times of water, and the water surface is splashed probiotics 50- per acre
It 100 grams, spilled once every 7-10 days.
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