CN104232497B - A kind of compound EM flora preparation and preparation method thereof for improving water quality of multi-cultur es - Google Patents
A kind of compound EM flora preparation and preparation method thereof for improving water quality of multi-cultur es Download PDFInfo
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Abstract
The invention belongs to microorganism field, in particular to the compound EM flora preparation and preparation method thereof for improving water quality of a kind of multi-cultur es.EM flora preparation of the invention contains Rhodopseudomonas palustris, lactobacillus acidophilus, saccharomyces cerevisiae, unwrapping wire aspergillus, bacillus subtilis, and total bacteria concentration in preparation is greater than 30 × 108Cfu/ml, wherein content >=60% of Rhodopseudomonas palustris；It is to be obtained after everfermentation, dilution by strain.Present invention is specifically directed to the environment of aquaculture, a kind of compound EM flora preparations for improving water quality of multi-cultur es of targeted breeding, harmful ammonia nitrogen, nitroso nitrogen in the water that can quickly, thoroughly degrade, the harmful substances such as removal of hydrogen sulfide；Residual bait and excreta in efficient-decomposition water eliminate sludge underwater, improve water quality；It can also keep probiotics quantity in water.
Description
Technical field
The invention belongs to microorganism field, in particular to the compound EM flora preparation and its system for improving water quality of a kind of multi-cultur es
Preparation Method.
Background technique
The society of raising with to(for) aquatic products awareness, more and more aquatic products become the deliciousness in people's disk
Delicacies.Culture fishery is also increasingly flourishing.But due to being on the rise for nowadays environmental pollution, cultivation density and scale are also
It is constantly soaring, increasingly higher demands are proposed to cultural technique.
EM bacterium, English name are " Effective Microorganisms ", it is selected from photosynthetic bacteria, actinomyces, ferment
Female bacterium, compound lactobacillus, bacillus etc. more than 10 belong to active bacteria formulation made of 80 multiple-microorganism compound criterias, in EM bacteria agent
The mutual symbiosis of various microorganisms, interaction, joint development play multiple functions, can quickly promote animals and plants residue
Equal organic material decompositions, inhibit the growth of spoilage organisms and other germs.
Present invention is specifically directed to the environment of aquaculture, a kind of targeted breeding multi-cultur es compound improvement water quality
EM flora preparation, harmful ammonia nitrogen, nitroso nitrogen, the harmful substances such as removal of hydrogen sulfide in the water that can quickly, thoroughly degrade；Efficiently
Residual bait and excreta in water are decomposed, sludge underwater is eliminated, improves water quality；Probiotics quantity in water can also be kept, is inhibited other
The field planting and invasion of pathogenic bacteria；Degradation of organic substances, effectively absorption harmful substance reduce water body acidity, stablize water pH value, supplement
Microelement improves water transparency, purifies water.
Summary of the invention
The technical problem to be solved by the present invention is providing a kind of multi-cultur es the compound EM flora preparation and its system for improving water quality
Preparation Method.
In the first aspect of the present invention, a kind of compound EM flora preparation for improving water quality of multi-cultur es is provided, marsh is contained
Red pseudomonas, lactobacillus acidophilus, saccharomyces cerevisiae, unwrapping wire aspergillus, bacillus subtilis, total bacteria concentration in preparation is greater than 30 ×
108Cfu/ml, wherein content >=60% of Rhodopseudomonas palustris.
In the second aspect of the present invention, a kind of preparation side of the compound EM flora preparation for improving water quality of multi-cultur es is provided
Method, characterized by comprising the steps of:
(1) preparation of culture solution
Sal-ammoniac (NH4Cl): sodium bicarbonate (NaHCO3): dipotassium hydrogen phosphate (K2HPO4): sodium acetate (CH3COONa): chlorination
Magnesium (MgCl2): sodium chloride (NaCl): glucose (C6H12O6): distilled water (H2O)=15:20:10:35:8:40:200:19672,
Sufficiently dissolution, it is spare to be made culture solution；
(2) preparation of composite probiotics preparations
The Rhodopseudomonas palustris (Rhodopseudomonas palustris) in photosynthetic bacteria is taken, strain content is 30
×108Cfu/ml or more；Lactobacillus acidophilus (Lactobacillus acidophilus) in lactic acid bacteria, strain content are 5
×108Cfu/ml or more；Saccharomyces cerevisiae (Saccharomyces cerevisiae) in saccharomycete, strain content be 20 ×
108Cfu/ml or more；Unwrapping wire aspergillus (Asporgillus cellulosae) strain content in filamentous fungi is 5 ×
106Cfu/ml or more；Bacillus subtilis (Bacillus subtilis), strain content are 60 × 108Cfu/ml or more；
Take the Rhodopseudomonas palustris of 8-12 parts (volume), 4-6 parts of (volume) lactobacillus acidophilus, 1-3 parts of (volume) brewer's yeasts, 1-2
Part (volume) unwrapping wire aspergillus, it is spare that strain is made in 1-3 parts of (volume) bacillus subtilises；
(3) preparation of compound mother bacterial liquid
The ratio of the 1:10-20 by volume of culture solution obtained by strain and step (1) obtained by step (2) is taken to pour into fermentor
In, kept for 25-30 DEG C of temperature, compound mother bacterial liquid is made in anaerobic fermentation culture 4-10 days；
(4) the compound mother bacterial liquid for taking step (3) to obtain is compounded with 40-60 times of deionized water, and compound bacteria dilution is made
Liquid is to get the compound EM flora preparation for improving water quality of multi-cultur es.
Since the fermentation of EM bacterium is due to the difference in season and fermentation time, the different product of color can be obtained, in order to make product
With uniform color and appearance, the dyestuff harmless to biology can also be used to compound bacteria dilution obtained in step (4)
It is dyed.
In addition to especially indicating, the number in the present invention refers to mass fraction.
Beneficial effects of the present invention: the compound efficacious microbial colony system for improving breeding water body water quality of multi-cultur es of the invention
Agent is by screening efficient water purification viable bacteria strain.This product liquid preparation, Viable detection is high, directly splashes, effect is obvious, makes
With very convenient；And harmful ammonia nitrogen, nitroso nitrogen, the harmful substances such as removal of hydrogen sulfide in the water that can quickly, thoroughly degrade；Efficiently
Residual bait and excreta in water are decomposed, sludge underwater is eliminated, improves water quality；Probiotics quantity in water can also be kept, is inhibited other
The field planting and invasion of pathogenic bacteria；Degradation of organic substances, effectively absorption harmful substance reduce water body acidity, stablize water pH value, supplement
Microelement improves water transparency, purifies water.
The efficacious microbial colony preparation of compound improvement breeding water body water quality of the invention has effects that as follows:
1, promote Abwehrkraft des Koepers: microbial flora preparation of the invention has the good activity for promoting aquaculture product
Ability improves its ability resisted the disease；
2, substrate: degraded pond bottom residual bait, excrement, animals and plants corpse and organic debris is improved；
3, it purifies water: the harmful substances such as effective adsorption tank bottom ammonia nitrogen, nitrite, hydrogen sulfide, algae toxin；
4, probiotics and planktonic organism proportional balancing method in water are kept, the flourish of harmful algae is inhibited；
5, water transparency is improved.
The efficacious microbial colony preparation of compound improvement breeding water body water quality of the invention has a characteristic that
1, the survival rate of this product liquid preparation, thallus is high, easy to use.
2, this product can save at normal temperature, avoid troublesome preserving type.
3, this product is adaptable, has stronger tolerance to chemical disinfection drug, insecticidal materials and antibiotic etc..
Specific embodiment
Embodiment 1
(1) preparation of culture solution
Sal-ammoniac (NH4Cl): sodium bicarbonate (NaHCO3): dipotassium hydrogen phosphate (K2HPO4): sodium acetate (CH3COONa): chlorination
Magnesium (MgCl2): sodium chloride (NaCl): glucose (C6H12O6): distilled water (H2O)=15:20:10:35:8:40:200:19672,
Sufficiently dissolution, it is spare to be made culture solution；
(2) preparation of composite probiotics preparations
The Rhodopseudomonas palustris (Rhodopseudomonas palustris) in photosynthetic bacteria is taken, strain content is 30
×108cfu/ml；Lactobacillus acidophilus (Lactobacillus acidophilus) in lactic acid bacteria, strain content be 5 ×
108cfu/ml；Brewer's yeast (Saccharomyces cerevisiae) in saccharomycete, strain content are 20 × 108cfu/
ml；Unwrapping wire aspergillus (Asporgillus cellulosae) strain content in filamentous fungi is 5 × 106cfu/ml；Withered grass bud
Spore bacillus (Bacillus subtilis), strain content are 60 × 108cfu/ml；The Rhodopseudomonas palustris of 12 parts of volumes is taken,
The lactobacillus acidophilus of 5 parts of volumes, the brewer's yeast of 2 parts of volumes, the unwrapping wire aspergillus of 1 part of volume, the bacillus subtilis of 2 parts of volumes
It is spare that strain is made；
(3) preparation of compound mother bacterial liquid
It takes the ratio of the 1:19 by volume of culture solution obtained by strain and step (1) obtained by step (2) to pour into fermentor, protects
25-30 DEG C of temperature is held, anaerobic fermentation culture 6 days, compound mother bacterial liquid is made；
(4) the compound mother bacterial liquid for taking step (3) to obtain is compounded with 49 times of deionized waters, and compound bacteria dilution is made,
It takes gardenia blue pigment and compound bacteria dilution to compound according to the ratio of mass ratio 1:1000 to be uniformly mixed, it is dilute that blue compound bacteria is made
Liquid is released up to the compound EM flora preparation for improving water quality of multi-cultur es.
Embodiment 2
Handle water body: the Penaeus Vannmei pool of two adjacent 25 mu of waters surface, the depth of water are 1 meter or so, when shrimp seedling is launched
Between be April 28, injected volume is 40,000 tails/mu, is divided into experimental group and control group, and process cycle is 2 months, every 10 days first 20 days
Using 1 time, water surface embodiment 1 obtains per acre EM flora preparation 200ml；Every 8 days 40 days with 1 time afterwards, per acre the water surface this
Product 450ml.Control group and experimental group feed feed twice daily during experiment, and morning 9:00 is fed once, and throw the dusk 17:00
It feeds once, other rearing conditions are all the same.After putting seedling from 20 days, control group and experimental group pond water quality index are surveyed, every 5 days
Measurement is primary, and tie-in 6 times, averaged (see the table below):
Claims (3)
1. a kind of compound EM flora preparation for improving water quality of multi-cultur es, it is characterised in that contain Rhodopseudomonas palustris, acidophilus cream
Bacillus, saccharomyces cerevisiae, unwrapping wire aspergillus, bacillus subtilis, total bacteria concentration in preparation are greater than 30 × 108Cfu/ml, wherein natural pond
Content >=60% of damp red pseudomonas；The EM flora preparation is prepared as follows to obtain: taking 8-12 parts of marsh red
Pseudomonad, 4-6 part lactobacillus acidophilus, 1-3 parts of brewer's yeasts, 1-2 parts of unwrapping wire aspergillus, 1-3 parts of bacillus subtilis mixing, system
It is spare to obtain strain；By gained strain and sal-ammoniac, sodium bicarbonate, dipotassium hydrogen phosphate, sodium acetate, magnesium chloride, sodium chloride, glucose
The ratio of the culture solution being configured to distilled water in 15:20:10:35:8:40:200:19672 1:10-20 by volume pours into hair
In fermentation tank, kept for 25-30 DEG C of temperature, compound mother bacterial liquid is made in anaerobic fermentation culture 4-10 days；By obtained compound mother bacterial liquid with
40-60 times of deionized water is diluted, and compound bacteria dilution is made to get the compound EM flora preparation for improving water quality of multi-cultur es.
2. a kind of method for preparing EM flora preparation as described in claim 1, characterized by comprising the steps of:
(1) preparation of culture solution
Sal-ammoniac (NH4Cl): sodium bicarbonate (NaHCO3): dipotassium hydrogen phosphate (K2HPO4): sodium acetate (CH3COONa): magnesium chloride
(MgCl2): sodium chloride (NaCl): glucose (C6H12O6): distilled water (H2O)=15:20:10:35:8:40:200:19672, fills
Divide dissolution, it is spare that culture solution is made；
(2) preparation of composite probiotics preparations
The Rhodopseudomonas palustris in photosynthetic bacteria is taken, strain content is 30 × 108cfu/ml；Lactobacillus acidophilus in lactic acid bacteria,
Strain content is 5 × 108cfu/ml；Saccharomyces cerevisiae in saccharomycete, strain content are 20 × 108cfu/ml；In filamentous fungi
Unwrapping wire aspergillus strain content is 5 × 106cfu/ml；Bacillus subtilis, strain content are 60 × 108cfu/ml；Take 8-12 parts
Rhodopseudomonas palustris, 4-6 part lactobacillus acidophilus, 1-3 parts of brewer's yeasts, 1-2 parts of unwrapping wire aspergillus, 1-3 parts of bacillus subtilises
It is spare that strain is made in mixing；
(3) preparation of compound mother bacterial liquid
It takes the ratio of the 1:10-20 by volume of culture solution obtained by strain and step (1) obtained by step (2) to pour into fermentor, protects
25-30 DEG C of temperature is held, compound mother bacterial liquid is made in anaerobic fermentation culture 4-10 days；
(4) the compound mother bacterial liquid for taking step (3) to obtain is diluted with 40-60 times of deionized water, compound bacteria dilution is made, i.e.,
Obtain the compound EM flora preparation for improving water quality of multi-cultur es.
3. the method for preparing EM flora preparation as claimed in claim 2, it is characterised in that further include taking gardenia blue pigment and answering
The combined bacteria dilution step uniformly mixed according to the ratio compounding of mass ratio 1:1000.
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| CN104745487A (en) * | 2015-03-25 | 2015-07-01 | 广东海纳川药业股份有限公司 | Complex microbial inoculant for water quality purification and application thereof |
| TWI676684B (en) * | 2017-06-15 | 2019-11-11 | 財團法人食品工業發展研究所 | Lactobacillus spp., method for producing pigment by using the same, a lactobacillus spp. culture and a pigment composition comprising the same |
| CN107364975A (en) * | 2017-07-10 | 2017-11-21 | 芜湖市木子生态园有限公司 | A kind of Aquacultural water modifying agent |
| CN107535393A (en) * | 2017-09-08 | 2018-01-05 | 无为县慧富水蛭养殖专业合作社 | A kind of degraded dispensing of hirudiniculture |
| CN108094283A (en) * | 2017-12-29 | 2018-06-01 | 海南中正水产科技有限公司 | A kind of method of indoor industrially biological breeding Penaeus Vannmei shrimp seedling |
| CN108587966B (en) * | 2018-05-07 | 2020-09-22 | 江苏大有生物科技发展有限公司 | Pure natural plant source EM (effective microorganisms) culture medium and application of fermentation product thereof in aquaculture |
| CN110713944A (en) * | 2018-07-12 | 2020-01-21 | 无锡三智生物科技有限公司 | Deodorizing microbial preparation for breeding house and manufacturing process |
| CN109430579A (en) * | 2018-12-10 | 2019-03-08 | 上海泓宝绿色水产股份有限公司 | EM bacterium calcium and magnesium compound micro-ecological preparation and preparation method thereof and application method |
| CN109607818A (en) * | 2018-12-19 | 2019-04-12 | 深圳合民生物科技有限公司 | A kind of microorganism improver of water quality and preparation method thereof |
| CN112812984B (en) * | 2019-11-18 | 2023-03-24 | 无锡泓宝微生物科技有限公司 | Preparation method and application method of compound microbial preparation for comprehensive planting and breeding of rice and fish |
| CN112280711A (en) * | 2020-11-02 | 2021-01-29 | 无锡泓宝微生物科技有限公司 | Water-regulating lovers for regulating and controlling water quality and preparation method thereof |
| CN112850913B (en) * | 2020-12-31 | 2023-04-18 | 上海泓宝绿色水产股份有限公司 | Bdellodicus bdellovi and preparation method and use method thereof |
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Address after: 200233 room 33, building 680, No. 303-1, Guiping Road, Shanghai, Xuhui District Applicant after: SHANGHAI HONGBAO GREEN AQUATIC PRODUCT CO., LTD. Applicant after: Shanghai Hongbao Science & Technology Co., Ltd. Address before: 200233 room 33, building 680, No. 303-1, Guiping Road, Shanghai, Xuhui District Applicant before: Shanghai Hongbao Green Aquatic Product Science & Technology Development Co., Ltd Applicant before: Shanghai Hongbao Science & Technology Co., Ltd. |
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Effective date of registration: 20191120 Address after: E Hu Zhen San Xin Cun Hong Bao Yuan, Xishan District, Wuxi City, Jiangsu Province Patentee after: Wuxi Hongbao Microorganism Technology Co., Ltd Address before: 200233 room 33, building 680, No. 303-1, Guiping Road, Shanghai, Xuhui District Co-patentee before: Shanghai Hongbao Science & Technology Co., Ltd. Patentee before: SHANGHAI HONGBAO GREEN AQUATIC PRODUCT CO., LTD. |