CN108094283A - A kind of method of indoor industrially biological breeding Penaeus Vannmei shrimp seedling - Google Patents

A kind of method of indoor industrially biological breeding Penaeus Vannmei shrimp seedling Download PDF

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Publication number
CN108094283A
CN108094283A CN201711473752.XA CN201711473752A CN108094283A CN 108094283 A CN108094283 A CN 108094283A CN 201711473752 A CN201711473752 A CN 201711473752A CN 108094283 A CN108094283 A CN 108094283A
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China
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water
seedling
stages
shrimp
pond
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CN201711473752.XA
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Chinese (zh)
Inventor
钟继伟
吴坤海
王平
李婷
符天山
曹飞飞
王其茂
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Hainan Zhong Zheng Aquatic Technology Co Ltd
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Hainan Zhong Zheng Aquatic Technology Co Ltd
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00—Culture of aquatic animals
    • A01K61/50—Culture of aquatic animals of shellfish
    • A01K61/59—Culture of aquatic animals of shellfish of crustaceans, e.g. lobsters or shrimps
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/20—Animal feeding-stuffs from material of animal origin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
    • C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81—Aquaculture, e.g. of fish

Abstract

The invention discloses a kind of method of indoor industrially biological breeding Penaeus Vannmei shrimp seedling, this method includes：White leg Shrimp seedling indoor industrially biological breeding flow and management method.This method is the innovation to traditional seedling-raising technique, the most healthy young of selection, put most rational density in a suitable place to breed, use the high-quality natural biological bait of the diatoms and fairy shrimp nauplius etc. such as micro- formula hailian seaweed, Chaetoceros muelleri, substitute the artificial fodder and bait in part, not only nutrition can have been enhanced and improved immunity, but also can effectively control nursery pond change of water quality, enhanced water body self-purification ability；Meanwhile inhibit harmful bacteria using beneficial bacteriums such as lactic acid bacteria, bacillus, pseudomonads and grow, residual bait, young remains etc. are decomposed, the stable nursery pond ecosystem is built with this.The high-quality shrimp seedling that this method can not only cultivate health, the speed of growth is fast, resistance is strong can also improve survival rate of seedling, save seedling cost, meet the seedling amount demand of client, so as to increase economic efficiency.

Description

A kind of method of indoor industrially biological breeding Penaeus Vannmei shrimp seedling
Technical field
The invention belongs to aquaculture fields, are related to a kind of breeding method of Penaeus Vannmei shrimp seedling, and in particular to a kind of The method of indoor industrially biological breeding Penaeus Vannmei shrimp seedling.
Background technology
In recent years, prawn culturing industry in China's rapidly develops, and Chinese prawn culturing yield accounts for the 1/3 of the world, occupies The first in the world, Penaeus Vannmei are the prawn culturing kinds of China's cultured area maximum；The shrimp seedling annual requirement of current China is about For 500,000,000,000 tails, and the shrimp seedling of Penaeus Vannmei is about 440,000,000,000 tails.Since 1992, the outburst of various virosis, bacterial disease Serious strike is brought to the development of world's shrimp industry.
Country's Penaeus Vannmei traditional mode of production is all single indoor cement pit pattern at present, and traditional seedling raising manners are all Using exogenous artitificial food and high-density breeding, daily ration, feeding quantity is big, and water quality is difficult to control, and ammonia nitrogen nitrous acid is excessively high to cause shrimp seedling to consume Bottom causes survival rate low.The nutrition of artitificial food does not have fresh and alive biological feed nutrition high, and nutrition, which is not caught up with, causes statement to be dragged It is phase, slow-growing.The organic contents such as residual bait, shrimp seedling metabolism, young remains are high, cause the bacterium of Miao Chili and germ grows and big The quick breeding of amount, so as to cause the appearance of various symptoms, in order to control the outburst of disease, often using substantial amounts of antibiotic, Furthermore high temperature nursery in addition fry quality in the market is irregular, carries the serious problems layer such as viral and slow-growing and goes out not Thoroughly.Cause substantial amounts of bait cost, antibiolics cost input is high, and shrimp seedling is of poor quality, and culture benefit is low, and such seed is Cannot meet the needs of raiser.Therefore the fry quality of prawn directly affects the speed of growth and survival rate in prawn later stage, together When one of an important factor for being also prawn culturing success or failure.
The content of the invention
The object of the present invention is to provide a kind of method of indoor industrially biological breeding Penaeus Vannmei shrimp seedling, this method Health can be cultivated, the high-quality shrimp seedling that the speed of growth is fast, inconsistent coefficient is small, resistance is strong, nursery can also be improved and survived Rate saves seedling cost, meets the seedling amount demand of client, so as to increase economic efficiency.
To achieve these goals, the technical scheme is that：It is white to provide a kind of indoor industrially biological breeding South America The method of prawn shrimp seedling, comprises the following steps：
(1) preparation before nursery：
A, disinfecting utensils：Utensil is placed in in the hydrochloric acid of 800~1200PPM disinfection 15~for 24 hours, by tunica albuginea, Air-regulating valves point Disinfection 15~for 24 hours is not placed in 200~300PPM povidone iodine；
B, workshop sterilizes：Points of 3 times progress of workshop cleaning and sterilizing, the 1st time with liquid detergent and 200~300PPM povidone iodine Each pond in workshop is cleaned, 1~2d of drying is then carried out, then closes door and window, with 800~1200PPM calcium hypochlorite disinfecting cars Between each corner；2nd cleaning and sterilizing, it is clear after 12~15h with liquid detergent and 200~300PPM povidone iodine to carry out pond It washes, gas stone, tracheae in pond is installed, rinsed well after being sterilized afterwards with 300~500PPM potassium permanganate with clear water；3rd Secondary disinfection is carried out disinfection with the potassium hydrogen persulfate of 800~1200PPM, tunica albuginea is covered after finishing；
C, water inlet and water body environment is adjusted：Nursery pond is indoor cement pit, is intake to 40~50 centimeters, add 100~ 100ppm sodium bicarbonate tune total alkalis add 30~40ppm EDTA complexation heavy metal ions to 160~170ppm；Water body index has been adjusted Cheng Hou supplements the nutritional amt of water body and addition beneficial bacterium with 2~3PPM prawn slice, the bottoming of 1.5~2.5PPM viable bacterias, adjusts water body Microenvironment, put before seedling detection water body water quality index and microbiological indicator；
(2) seedling is put：The nauplius of high-quality health is selected, the young 10 is cleaned with 200~300PPM povidone iodine before launching ~15s；Seedling density is put as 20~250,000 tails/m3；
(3) nursery and management：
A, biological feed is fed：The abnormal situation of the young will be observed the moment by putting the N5 young, it is found that the young begins with metamorphosis When, start to feed micro- formula hailian seaweed and Chaetoceros muelleri, the concentration of micro- formula hailian seaweed is 2.0 × 105~3.0 × 105A/ml, The concentration of Chaetoceros muelleri is 5.0 × 105~6.0 × 105A/ml starts to feed 17~25g/m to the Z2 middle and later periods3Fairy shrimp Nauplius；
B, man-made feeds are fed：Start to feed artitificial food in the middle and later periods of Z2, artitificial food include dimension benevolence prawn slice, AK808, tall and handsome No. 1 material and tall and handsome No. 2 material, wherein：It is 2.5~5.5g/m that the Z2 stages, which feed dimension benevolence prawn slice,3, AK808 0.8 ~1.3g/m3, tall and handsome No. 1 material is 1.0~2.0g/m3；It is 4~7g/m that the Z3 stages, which feed dimension benevolence prawn slice,3, AK808 for 0.8~ 1.3g/m3, tall and handsome No. 1 material is 1.0~2.0g/m3；It is 7~12g/m that M1~M3 stages, which feed and feed dimension benevolence prawn slice,3, tall and handsome No. 2 material For 1.0~2.0g/m3；It is 10~15 grams of g/m that P1~P5 stages, which feed dimension benevolence prawn slice,3；It to splash during each feeding uniformly；
C, water is changed in workshop：The Z3 later stages start plus fresh water, promote the development by metamorphosis of shrimp seedling, start to change water to the M2 stages, change water It measures as 25%~35% of water volume in pond；
D, beneficial bacterium is added：Nursery metamorphosis started to add 25~30PPM pseudomonads daily to Z2~Z3 stages, 10~ 15PPM lactic acid bacterias and 15~25PPM bacillus；
E, shrimp seedling detects：To pass through in the process shrimp seedling of nursery and strictly detect, respectively the Z2 stages, the M2 stages, the P2 stages, P7 stages, four-stage detect the water quality index and microbiological indicator of shrimp seedling, ensure following index：Water temperature keeps 30 DEG C~32 DEG C, pH value 7.8~8.8, COD COD<3mg/L, ammonia nitrogen≤0.1ppm, nitrite≤0.1ppm, dissolved oxygen DO> 8mg/L, chlorine residue≤0.001ppm, total phosphorus<0.1mg/L, 120~180ppm of total alkali, vibrios yellow color colonies in water body≤ 103Cfu/ml, green flora≤102Cfu/ml, bacterium:Vibrios >=10:1；Shrimp body vibrios yellow color colonies≤104Cfu/ml, green Flora≤103Cfu/ml, bacterium:Vibrios >=10:1；
F, water quality adjustment：When detecting water quality and unqualified microorganism, the former water of 30% volume in pond is changed fresh Water, change after water and put into 10~15PPM povidone iodine disinfection water body, then put into again 15~30PPM lactic acid bacteria and 15~ The bacillus of 30PPM nurses one's health water quality, inhibits harmful bacteria using beneficial bacterium, improves the immunity of shrimp seedling and shrimp seedling quality； Continuous so operate 1~2 time just substantially can handle shrimp seedling well；
(4) emerge：It is 0~20 ‰ to adjust salinity in seedling pond during emergence in advance, and emergence water temperature is 20~25 DEG C, drags for Miao Shiyao Pay special attention to, action is light, avoids hindering seedling.
The method of indoor industrially biological breeding Penaeus Vannmei shrimp seedling of the present invention has following advantageous effect：
1st, applied ecology principle of the present invention is 2.0 × 10 using concentration5~3.0 × 105The micro- formula hailian seaweeds of a/ml are dense It spends for 5.0 × 105~6.0 × 105Diatoms and the 17~25g/m such as/ml Chaetoceros muelleris3Fairy shrimp nauplius etc. it is natural raw Object bait substitutes part artitificial food, and wherein Chaetoceros, hailian seaweed are the higher species of nutritional ingredient in diatom, and individual is small, Easily digestion is one of optimum open-mouthed bait of the shrimps young, and fairy shrimp nauplius has substantial amounts of yolk, and containing rich Rich protein and fat (note:Wherein protein containing about 60%, fat is containing about the nutrition that 20%) can sufficiently meet shrimp seedling Demand, the addition of natural biological bait can not only enhance nutrition and improve immunity, but also can effectively control the change of water quality of nursery pond, increase The self-purification capacity of strong water body.
2nd, shrimp seed rearing of the present invention is false single using 10~15PPM lactic acid bacterias, 15~25PPM bacillus, 25~30PPM The beneficial bacteriums such as born of the same parents bacterium inhibit harmful bacteria and grow, and residual bait, remains young in decomposing pool etc. further maintains the nursery pond ecosystem Stablize.
3rd, the present invention can save substantial amounts of bait cost, and no matter in survival rate or shrimp seedling quality have larger carry It rises, it is average more than 60% using the technology survival rate of biological breeding shrimp seedling, more than 90% can be reached.Compared to more traditional The survival rate of nursery has obvious advantage.
4th, the present invention is applied to using the biotechnology of health, ecology among entire nursery, has been completely free of to antibiotic The dependence of drugs is waited, establishes a kind of health, the nursery pattern of ecology.
5th, the present invention selects suitable PH, salinity, temperature, intensity of illumination, total alkalinity, bait, Rational Stocking density and is good for The management mode of health, rationally inhibits the growth of harmful bacteria using probiotics, and regulating water quality activates water body, decomposes the residual of water body Slag, residual bait, remains reduce the ammonia nitrogen of water body and nitrous acid content, so as to improve shrimp seedling quality and survival rate of seedling.
Specific embodiment
Embodiment 1
A kind of method of indoor industrially biological breeding Penaeus Vannmei shrimp seedling, which is characterized in that comprise the following steps：
(1) preparation before nursery：
A, disinfecting utensils：Utensil is placed in the hydrochloric acid of 800PPM and sterilizes 15h, tunica albuginea, Air-regulating valves are respectively placed in 15h is sterilized in 200PPM povidone iodine；
B, workshop sterilizes：3 progress of workshop cleaning and sterilizing point, the 1st time with liquid detergent and 200PPM povidone iodine workshop Each pond cleaning, then carry out drying 1d, then close door and window, with 800PPM calcium hypochlorites disinfection workshop each corner； 2nd cleaning and sterilizing is cleaned with liquid detergent and 200PPM povidone iodine progress pond after 12h, gas stone, tracheae in pond is installed Finish, rinsed well after being sterilized afterwards with 300PPM potassium permanganate with clear water；3rd disinfection, with the potassium hydrogen persulfate of 800PPM into Row disinfection, after finishing covers tunica albuginea；
C, water inlet and water body environment is adjusted：An indoor raising of seedling cement pit is selected, 12 long 4m, wide 3m, high 1.5m's educates Miao Chi is intake to 40 centimeters, and 100ppm sodium bicarbonate tune total alkalis is added to add 30ppm EDTA complexation heavy metal ions to 160ppm；Water After the completion of body index is adjusted, it is beneficial to supplement the nutritional amt of water body and addition with 2PPM prawn slice, the logical viable bacteria bottoming of the peaces of 1.5PPM hundred Bacterium adjusts the microenvironment of water body, puts the water quality index and microbiological indicator of before seedling detection water body；
(2) seedling is put：Workshop water temperature adds to 32 DEG C, selects the nauplius of high-quality health, and 200PPM povidone is used before launching Iodine cleaning young 10s；2,000,000 young are put per pond；
(3) nursery and management：
A, biological feed is fed：The abnormal situation of the young will be observed the moment by putting the N5 young, and the 6 o'clock metamorphosis of the young morning is opened Beginning feeds 8 tons of micro- formula hailian seaweeds and 8 tons of Chaetoceros muelleris, and the concentration of Chaetoceros muelleri is 5.0 × 105A/ml, micro- formula sea chain The concentration of algae is 2.0 × 105/ml, adds 1.4 tons of algae water per pond, and the algae density in nursery pond is 6.8 × 104A/ml, (educates Algae water density in Miao Chi is maintained at 5.0 × 104/ ml~7.0 × 104/ ml), it is timely that the flea shape stage finds that density reduces Add, the algae in pond using direct projection into sunlight spread cultivation to seedling pond scope, further meet the needs of shrimp seedling is to algae, accord with Close Principles of Ecosystem；Start to feed 17g/m to the Z2 middle and later periods3Fairy shrimp nauplius；
B, man-made feeds are fed：Start to feed artitificial food in the middle and later periods of Z2, artitificial food include dimension benevolence prawn slice, AK808, tall and handsome No. 1 material and tall and handsome No. 2 material, wherein：It is 2.5g/ m that the Z2 stages, which feed dimension benevolence prawn slice,3, AK808 0.8g/m3, English Big No. 1 material is 1.0g/m3；It is 4g/ m that the Z3 stages, which feed dimension benevolence prawn slice,3, AK808 0.8g/m3, tall and handsome No. 1 material is 1.0g/m3； It is 7g/m that M1~M3 stages, which feed and feed dimension benevolence prawn slice,3, tall and handsome No. 2 material are 1.0g/m3；It is 10 that P1~P5 stages, which feed dimension benevolence prawn slice, Gram g/m3；It to splash during each feeding uniformly；(AK808, tall and handsome No. 1 material and tall and handsome No. 2 material are the product to circulate on the market, AK808 produces for TaiWan, China Lv Qi international businesses Co., Ltd, and tall and handsome No. 1 and tall and handsome No. 2 is the tall and handsome company's lifes of INVE Production)
C, water is changed in workshop：The Z3 later stages start plus fresh water, promote the development by metamorphosis of shrimp seedling, start to change water to the M2 stages, change water It measures as 25% of water volume in pond；
D, beneficial bacterium is added：Nursery metamorphosis started to add 25PPM pseudomonads, 10PPM lactic acid to Z2~Z3 stages daily Bacterium and 15PPM bacillus；With the happy multi-source microorganism formulations of 20PPM come regulating water quality when water quality is bad, water quality is made to be kept for one Pure and fresh state；
E, shrimp seedling detects：To pass through in the process shrimp seedling of nursery and strictly detect, respectively the Z2 stages, the M2 stages, the P2 stages, P7 stages, four-stage detect the water quality index and microbiological indicator of shrimp seedling, ensure following index：Water temperature is kept for 30 DEG C, pH value 7.8, COD COD<3mg/L, ammonia nitrogen≤0.1ppm, nitrite≤0.1ppm, dissolved oxygen DO>8mg/L, chlorine residue≤ 0.001ppm, total phosphorus<0.1mg/L, 120~180ppm of total alkali, vibrios yellow color colonies≤10 in water body3Cfu/ml, green flora ≤102Cfu/ml, bacterium:Vibrios >=10:1；Shrimp body vibrios yellow color colonies≤104Cfu/ml, green flora≤103Cfu/ml, Bacterium:Vibrios >=10:1；
F, water quality adjustment：When detecting water quality and unqualified microorganism, the former water of 30% volume in pond is changed fresh Water, change after water and put into 10PPM povidone iodine disinfection water body, then put into the lactic acid bacteria of 15PPM and the bacillus of 15PPM again Water quality is nursed one's health, inhibits harmful bacteria using beneficial bacterium, improves the immunity of shrimp seedling and shrimp seedling quality；
(4) emerge：It is 10 ‰ to adjust salinity in seedling pond during emergence in advance, and emergence water temperature is 20 DEG C, especially to be noted when dragging for seedling Meaning, action is light, avoids hindering seedling.
For the shrimp seedling survival rate of the present embodiment 1 80%, tracking 1 shrimp seedling of the present embodiment is fast to the cultivation length speed outside the pool, and 100 It can grow to 30 first jin, and yield is high, compare the cultivation of other shrimp seedlings, and the shrimp seedling quality that this method is cultivated is high.
Embodiment 2
A kind of method of indoor industrially biological breeding Penaeus Vannmei shrimp seedling, comprises the following steps：
(1) preparation before nursery：
A, disinfecting utensils：Utensil is placed in the hydrochloric acid of 1000PPM and sterilizes 20h, tunica albuginea, Air-regulating valves are respectively placed in 20h is sterilized in 250PPM povidone iodine；
B, workshop sterilizes：3 progress of workshop cleaning and sterilizing point, the 1st time with liquid detergent and 250PPM povidone iodine workshop Each pond cleaning, then carry out drying 2d, then close door and window, with 1000PPM calcium hypochlorites disinfection workshop each corner； 2nd cleaning and sterilizing is cleaned with liquid detergent and 250PPM povidone iodine progress pond after 14h, gas stone, tracheae in pond is installed Finish, rinsed well after being sterilized afterwards with 400PPM potassium permanganate with clear water；3rd disinfection, with the potassium hydrogen persulfate of 1000PPM into Row disinfection, after finishing covers tunica albuginea；
C, water inlet and water body environment is adjusted：An indoor raising of seedling cement pit is selected, 12 long 4m, wide 3m, high 1.5m's educates Miao Chi, each pond intake to 50 centimeters, add 120ppm sodium bicarbonate tune total alkalis to 170ppm, add 35ppm EDTA complexing heavy metals from Son；After the completion of water body index is adjusted, it is beneficial to supplement the nutritional amt of water body and addition with 3PPM prawn slice, the bottoming of 2.0PPM viable bacterias Bacterium adjusts the microenvironment of water body, puts the water quality index and microbiological indicator of before seedling detection water body；
(2) seedling is put：Workshop water temperature adds to 32 DEG C, selects the nauplius of high-quality health, and 250PPM povidone is used before launching Iodine cleaning young 13s；2,200,000 young are put per pond；
(3) nursery and management：
A, biological feed is fed：The abnormal situation of the young will be observed the moment by putting the N5 young, be started at 10 o'clock of the young morning There is metamorphosis, start to feed 10 tons of micro- formula hailian seaweed and 10 tons of Chaetoceros muelleri, the concentration of micro- formula hailian seaweed is 3.0 × 105 A/ml, the concentration of Chaetoceros muelleri is 6.0 × 105/ ml starts to feed 20g/m to the Z2 middle and later periods3Fairy shrimp nauplius；
B, man-made feeds are fed：Start to feed artitificial food in the middle and later periods of Z2, artitificial food include dimension benevolence prawn slice, AK808, tall and handsome No. 1 material and tall and handsome No. 2 material, wherein：It is 4g/ m that the Z2 stages, which feed dimension benevolence prawn slice,3, AK808 1.0g/m3, it is tall and handsome No. 1 material is 1.5g/m3；It is 5g/ m that the Z3 stages, which feed dimension benevolence prawn slice,3, AK808 1.2g/m3, tall and handsome No. 1 material is 1.5g/m3；M1 It is 10g/m that the~M3 stages, which feed and feed dimension benevolence prawn slice,3, tall and handsome No. 2 material are 1.5g/m3；It is 13 grams that P1~P5 stages, which feed dimension benevolence prawn slice, g/m 3；It to splash during each feeding uniformly；
C, water is changed in workshop：The Z3 later stages start plus fresh water, promote the development by metamorphosis of shrimp seedling, start to change water to the M2 stages, change water It measures as 30% of water volume in pond；
D, beneficial bacterium is added：Nursery is abnormal to start to add 28PPM pseudomonads to Z2~Z3 stages, 12PPM lactic acid bacterias with 20PPM bacillus；With the happy multi-source microorganism formulations of 30PPM come regulating water quality when water quality is bad, make water quality keep one it is pure and fresh State.
E, shrimp seedling detects：To pass through in the process shrimp seedling of nursery and strictly detect, respectively the Z2 stages, the M2 stages, the P2 stages, P7 stages, four-stage detect the water quality index and microbiological indicator of shrimp seedling, ensure following index：Water temperature is kept for 32 DEG C, pH value 8.0, COD COD<3mg/L, ammonia nitrogen≤0.1ppm, nitrite≤0.1ppm, dissolved oxygen DO>8mg/L, chlorine residue≤ 0.001ppm, total phosphorus<0.1mg/L, total alkali 160ppm, vibrios yellow color colonies≤10 in water body3Cfu/ml, green flora≤ 102Cfu/ml, bacterium:Vibrios >=10:1；Shrimp body vibrios yellow color colonies≤104Cfu/ml, green flora≤103Cfu/ml, carefully Bacterium:Vibrios >=10:1；
F, water quality adjustment：When detecting water quality and unqualified microorganism, the former water of 30% volume in pond is changed fresh Water, change after water and put into 12PPM povidone iodine disinfection water body, then put into the lactic acid bacteria of 12PPM and the bacillus of 12PPM again Water quality is nursed one's health, inhibits harmful bacteria using beneficial bacterium, improves the immunity of shrimp seedling and shrimp seedling quality；It is continuous so to operate 2 times Shrimp seedling can just be handled well substantially；
(4) emerge：It is 15 ‰ to adjust salinity in seedling pond during emergence in advance, and emergence water temperature is 25 DEG C, especially to be noted when dragging for seedling Meaning, action is light, avoids hindering seedling.
For the shrimp seedling survival rate of the present embodiment 2 85%, tracking 2 shrimp seedling of the present embodiment is fast to the cultivation length speed outside the pool, and 100 It can grow to 32 first jin, and yield is high, compare the cultivation of other shrimp seedlings, and the shrimp seedling quality that this method is cultivated is high.
Embodiment 3
A kind of method of indoor industrially biological breeding Penaeus Vannmei shrimp seedling, comprises the following steps：
(1) preparation before nursery：
A, disinfecting utensils：Utensil is placed in the hydrochloric acid of 1200PPM and sterilized for 24 hours, tunica albuginea, Air-regulating valves are respectively placed in It is sterilized for 24 hours in 300PPM povidone iodine；
B, workshop sterilizes：3 progress of workshop cleaning and sterilizing point, the 1st time with liquid detergent and 300PPM povidone iodine workshop Each pond cleaning, then carry out drying 2d, then close door and window, with 1200PPM calcium hypochlorites disinfection workshop each corner； 2nd cleaning and sterilizing is cleaned with liquid detergent and 300PPM povidone iodine progress pond after 15h, gas stone, tracheae in pond is installed Finish, rinsed well after being sterilized afterwards with 500PPM potassium permanganate with clear water；3rd disinfection, with the potassium hydrogen persulfate of 1200PPM into Row disinfection, after finishing covers tunica albuginea；
C, water inlet and water body environment is adjusted：An indoor raising of seedling cement pit is selected, 12 long 4m, wide 3m, high 1.5m's educates Miao Chi is intake to 50 centimeters, and 150ppm sodium bicarbonate tune total alkalis is added to add 40ppm EDTA complexation heavy metal ions to 170ppm；Water After the completion of body index is adjusted, the nutritional amt of water body and addition beneficial bacterium are supplemented with 3PPM prawn slice, the bottoming of 2.5PPM viable bacterias, is adjusted The microenvironment of water body puts the water quality index and microbiological indicator of before seedling detection water body；
(2) seedling is put：Workshop water temperature adds to 32 DEG C, selects the nauplius of high-quality health, and 300PPM povidone is used before launching 10~15s of the iodine cleaning young；2,500,000 young are put per pond；
(3) nursery and management：
A, biological feed is fed：The abnormal situation of the young will be observed the moment by putting the N5 young, and the morning, the 10 point discovery young opened There is metamorphosis in beginning, starts to feed the micro- formula hailian seaweed of 5 tons of tons and 5 tons of Chaetoceros muelleris, the concentration of micro- formula hailian seaweed is 3.0 × 105A/ Ml, the concentration of Chaetoceros muelleri is 6.0 × 105/ ml starts to feed 25g/m to the Z2 middle and later periods3Fairy shrimp nauplius；
B, man-made feeds are fed：Start to feed artitificial food in the middle and later periods of Z2, artitificial food include dimension benevolence prawn slice, AK808, tall and handsome No. 1 material and tall and handsome No. 2 material, wherein：It is 5.5 g/m that the Z2 stages, which feed dimension benevolence prawn slice,3, AK808 1.3g/m3, English Big No. 1 material is 2.0g/m3；It is 7g/m that the Z3 stages, which feed dimension benevolence prawn slice,3, AK808 1.3g/m3, tall and handsome No. 1 material is 2.0g/m3； It is 12g/m that M1~M3 stages, which feed and feed dimension benevolence prawn slice,3, tall and handsome No. 2 material are 2.0g/m3；It is 15 that P1~P5 stages, which feed dimension benevolence prawn slice, Gram g/m3；It to splash during each feeding uniformly；
C, water is changed in workshop：The Z3 later stages start plus fresh water, promote the development by metamorphosis of shrimp seedling, start to change water to the M2 stages, change water It measures as 35% of water volume in pond；
D, beneficial bacterium is added：Nursery is abnormal to start to add 30PPM pseudomonads to Z2~Z3 stages, 15PPM lactic acid bacterias with 25PPM bacillus；With the happy multi-source microorganism formulations of 30PPM come regulating water quality when water quality is bad, make water quality keep one it is pure and fresh State；
E, shrimp seedling detects：To pass through in the process shrimp seedling of nursery and strictly detect, respectively the Z2 stages, the M2 stages, the P2 stages, P7 stages, four-stage detect the water quality index and microbiological indicator of shrimp seedling, ensure following index：Water temperature is kept for 32 DEG C, pH value 8.8, COD COD<3mg/L, ammonia nitrogen≤0.1ppm, nitrite≤0.1ppm, dissolved oxygen DO>8mg/L, chlorine residue≤ 0.001ppm, total phosphorus<0.1mg/L, total alkali 180ppm, vibrios yellow color colonies≤10 in water body3Cfu/ml, green flora≤ 102Cfu/ml, bacterium:Vibrios >=10:1；Shrimp body vibrios yellow color colonies≤104Cfu/ml, green flora≤103Cfu/ml, carefully Bacterium:Vibrios >=10:1；
F, water quality adjustment：When detecting water quality and unqualified microorganism, the former water of 30% volume in pond is changed fresh Water, change after water and put into 15PPM povidone iodine disinfection water body, then put into the lactic acid bacteria of 30PPM and the bacillus of 30PPM again Water quality is nursed one's health, inhibits harmful bacteria using beneficial bacterium, improves the immunity of shrimp seedling and shrimp seedling quality；It is continuous so to operate 2 times Shrimp seedling can just be handled well substantially；
(4) emerge：It is 20 ‰ to adjust salinity in seedling pond during emergence in advance, and emergence water temperature is 25 DEG C, especially to be noted when dragging for seedling Meaning, action is light, avoids hindering seedling.
For the shrimp seedling survival rate of the present embodiment 3 90%, tracking 3 shrimp seedling of the present embodiment is fast to the cultivation length speed outside the pool, and 100 It can grow to 35 first jin, and yield is high, compare the cultivation of other shrimp seedlings, and the shrimp seedling quality that this method is cultivated is high.
The above disclosed right for being only presently preferred embodiments of the present invention, the present invention cannot being limited with this certainly Scope, therefore equivalent variations made according to the claims of the present invention still fall within the scope that the present invention is covered.

Claims (1)

  1. A kind of 1. method of indoor industrially biological breeding Penaeus Vannmei shrimp seedling, which is characterized in that comprise the following steps：
    (1) preparation before nursery：
    A, disinfecting utensils：Utensil is placed in in the hydrochloric acid of 800~1200PPM disinfection 15~for 24 hours, tunica albuginea, Air-regulating valves are put respectively In 200~300PPM povidone iodine disinfection 15~for 24 hours；
    B, workshop sterilizes：3 progress of workshop cleaning and sterilizing point, the 1st time with liquid detergent and 200~300PPM povidone iodine workshop Each pond cleaning, then carry out 1~2d of drying, then close door and window, with 800~1200PPM calcium hypochlorites disinfection workshop it is every One corner；2nd cleaning and sterilizing is cleaned after 12~15h with liquid detergent and 200~300PPM povidone iodine progress pond, will Gas stone, tracheae install in pond, are rinsed well after being sterilized afterwards with 300~500PPM potassium permanganate with clear water；Disappear for 3rd time Poison is carried out disinfection with the potassium hydrogen persulfate of 800~1200PPM, tunica albuginea is covered after finishing；
    C, water inlet and water body environment is adjusted：Nursery pond is indoor cement pit, intakes to 40~50 centimeters, adds 100~100ppm small Soda tune total alkali adds 30~40ppm EDTA complexation heavy metal ions to 160~170ppm；After the completion of water body index is adjusted, with 2 ~3PPM prawn slice, the bottoming of 1.5~2.5PPM viable bacterias supplement the nutritional amt of water body and addition beneficial bacterium, adjust the micro-loop of water body The water quality index and microbiological indicator of before seedling detection water body are put in border；
    (2) seedling is put：Select the nauplius of high-quality health, before launching with 200~300PPM povidone iodine clean the young 10~ 15s；Seedling density is put as 20~250,000 tails/m3；
    (3) nursery and management：
    A, biological feed is fed：The abnormal situation of the young will be observed the moment by putting the N5 young, it is found that when the young begins with metamorphosis, be opened Beginning feeds micro- formula hailian seaweed and Chaetoceros muelleri, and the concentration of micro- formula hailian seaweed is 2.0 × 105~3.0 × 105A/ml, Mu Shi angle The concentration of hair algae is 5.0 × 105~6.0 × 105A/ml starts to feed 17~25g/m to the Z2 middle and later periods3Fairy shrimp without section children Body；
    B, man-made feeds are fed：Start to feed artitificial food in the middle and later periods of Z2, artitificial food include dimension benevolence prawn slice, AK808, Tall and handsome No. 1 material and tall and handsome No. 2 material, wherein：It is 2.5~5.5g/m that the Z2 stages, which feed dimension benevolence prawn slice,3, AK808 is 0.8~1.3g/ m3, tall and handsome No. 1 material is 1.0~2.0g/m3；It is 4~7g/m that the Z3 stages, which feed dimension benevolence prawn slice,3, AK808 is 0.8~1.3g/m3, English Big No. 1 material is 1.0~2.0g/m3；It is 7~12g/m that M1~M3 stages, which feed and feed dimension benevolence prawn slice,3, tall and handsome No. 2 material for 1.0~ 2.0g/m3；It is 10~15 grams of g/m that P1~P5 stages, which feed dimension benevolence prawn slice,3；It to splash during each feeding uniformly；
    C, water is changed in workshop：The Z3 later stages start plus fresh water, promote the development by metamorphosis of shrimp seedling, start to change water to the M2 stages, quantity of exchanged water is The 25%~35% of water volume in pond；
    D, beneficial bacterium is added：Nursery metamorphosis started to add 25~30PPM pseudomonads, 10~15PPM daily to Z2~Z3 stages Lactic acid bacteria and 15~25PPM bacillus；
    E, shrimp seedling detects：To pass through stringent detection, respectively Z2 stages, M2 stages, P2 stages, P7 ranks in the process shrimp seedling of nursery Section, four-stage detect the water quality index and microbiological indicator of shrimp seedling, ensure following index：Water temperature is kept for 30 DEG C~32 DEG C, pH Value 7.8~8.8, COD COD<3mg/L, ammonia nitrogen≤0.1ppm, nitrite≤0.1ppm, dissolved oxygen DO>8mg/L, Chlorine residue≤0.001ppm, total phosphorus<0.1mg/L, 120~180ppm of total alkali, vibrios yellow color colonies≤10 in water body3Cfu/ml is green Color flora≤102Cfu/ml, bacterium:Vibrios >=10:1；Shrimp body vibrios yellow color colonies≤104Cfu/ml, green flora≤ 103Cfu/ml, bacterium:Vibrios >=10:1；
    F, water quality adjustment：When detecting water quality and unqualified microorganism, the former water of 30% volume in pond is changed into fresh water, It changes after water and puts into 10~15PPM povidone iodine disinfection water body, then put into lactic acid bacteria and the 15~30PPM of 15~30PPM again Bacillus nurses one's health water quality, inhibits harmful bacteria using beneficial bacterium, improves the immunity of shrimp seedling and shrimp seedling quality；
    (4) emerge：It is 0~20 ‰ to adjust salinity in seedling pond during emergence in advance, and emergence water temperature is 20~25 DEG C, is dragged for special during seedling Note that action is light, avoid hindering seedling.
CN201711473752.XA 2017-12-29 2017-12-29 A kind of method of indoor industrially biological breeding Penaeus Vannmei shrimp seedling Pending CN108094283A (en)

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CN108834960A (en) * 2018-08-07 2018-11-20 海南中正水产科技有限公司 A kind of high yield incubation method of Penaeus Vannmei nauplius
CN109644909A (en) * 2018-12-08 2019-04-19 海南海壹水产种苗有限公司 A kind of device and method applied to shrimp nauplius miniflow water cleaning and sterilizing
CN110679524A (en) * 2019-09-19 2020-01-14 海南海壹水产种苗有限公司 Indoor industrialized ecological specific pathogen-free seedling cultivation method for litopenaeus vannamei
CN113589871A (en) * 2021-07-27 2021-11-02 渤海水产育种(海南)有限公司 Seed shrimp breeding and breeding system

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CN109644909A (en) * 2018-12-08 2019-04-19 海南海壹水产种苗有限公司 A kind of device and method applied to shrimp nauplius miniflow water cleaning and sterilizing
CN110679524A (en) * 2019-09-19 2020-01-14 海南海壹水产种苗有限公司 Indoor industrialized ecological specific pathogen-free seedling cultivation method for litopenaeus vannamei
CN113589871A (en) * 2021-07-27 2021-11-02 渤海水产育种(海南)有限公司 Seed shrimp breeding and breeding system

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Application publication date: 20180601