CN109644909A - A kind of device and method applied to shrimp nauplius miniflow water cleaning and sterilizing - Google Patents
A kind of device and method applied to shrimp nauplius miniflow water cleaning and sterilizing Download PDFInfo
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- CN109644909A CN109644909A CN201811498611.8A CN201811498611A CN109644909A CN 109644909 A CN109644909 A CN 109644909A CN 201811498611 A CN201811498611 A CN 201811498611A CN 109644909 A CN109644909 A CN 109644909A
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- nauplius
- water
- young
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- miniflow
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/59—Culture of aquatic animals of shellfish of crustaceans, e.g. lobsters or shrimps
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/60—Floating cultivation devices, e.g. rafts or floating fish-farms
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention belongs to field of aquaculture, especially a kind of device and method applied to shrimp nauplius miniflow water cleaning and sterilizing, described device includes temporarily supporting bucket and circular cylinder cage, the circular cylinder cage is placed in temporarily feeding bucket and is higher by the temporarily top of feeding bucket along 20cm, the circular cylinder cage includes support frame, the cylindrical string bag and lashing, the support frame is made of stainless steel tube welding, edge is equipped with lashing with bottom on the cylinder string bag, the cylinder string bag is fixed by lashing and support frame, fit closely the entire cylindrical string bag with support frame, the method of miniflow water cleaning and sterilizing of the present invention can make the damage ratio of the young and stick dirty rate reduction by 20%~40%, the vibrios carrying amount of the young reduces by 50%~80%, it is very suitable to the utilization of nauplius actual production, to optimize nothing The quality for saving the young, effectively facilitates the survival rate of seedling of the young.
Description
Technical field
It is especially a kind of applied to shrimp nauplius miniflow water cleaning and sterilizing the invention belongs to field of aquaculture
Device and method.
Background technique
Nauplius refers to that certain crustaceans (such as Copepods, cirrus class, krill class and Decapoda branch gill class) just incubate
The young out.Shrimp nauplius is then just to start to occur in the higher stage of development, and body is not divided into cephalothorax and abdomen still, is in
Flat elliptic has nauplius eye 1 in front of median line, and there are mouth and digest tube (anus is not yet turned on) in rear, left and right
3 pairs of appendages, the appendages such as tool antennule, antenna secunda and big jaw are made of several joints, tool swimming bristle.
Nauplius is most basic link in shrimp species seedling production process, and whether the superiority and inferiority of Larval quality carries infection disease
Original etc. directly affects survival rate of seedling, so that nursery effect is influenced, therefore the problem of current shrimp nauplius quality, still restricts
How the further expansion of prawn culturing production and the raising of prawn Seedling production Business Economic Benefit, improve nauplius quality
Correlative study seem very necessary.Therefore, there is an urgent need to be picked up from source, cleaning and sterilizing is carried out to nauplius, reduces nothing
The carrying amount for saving young organic matter and microorganism, prevents vibrio infection from propagating, so that preventing cause of disease is carried to nursery workshop.Many institutes
Known, the temporary link of supporting after having collected nauplius is to guarantee one extremely important step of Larval quality, about this ring
Probing into for section is not reported substantially, typically carrys out control by the operating experience of seed shrimp technician, operating method is nearly all
It is to come down in a continuous line, traditional method is to carry out to the temporary feeding young plus change water (to fish out the young from a bucket by reducing temporarily feeding density
To another bucket), but be easy that the young is caused to damage, influence the quality of the young.
Such as Chinese patent application application number 201611160264.9, it is preferably square to disclose a kind of Penaeus Vannmei nauplius
Fertilized eggs are put into Lotic hatching bucket and incubated by method after this method uses 0.5ppm chlorine, 50ppm povidone iodine disinfection fertilized eggs first
It is melted into nauplius, there are the risks that disinfectant influences incubating oosperm, especially hatching shadow of the chlorine residue to fertilized eggs for this method
It rings very big.For another example Chinese patent application application number 201620950026.7,201620950025.2 and 201610731644.7,
All it is the screening plant and method about nauplius, the disinfection of nauplius is then not involved with.For another example Chinese patent Shen
Please application number 201520851280.7, disclose a kind of freshwater shrimp miniflow water cultivation apparatus, including glass jar, water feed apparatus and water outlet
Device, it is characterised in that: water feed apparatus includes water inlet pipe and micro liquid flowmeter, for being discharged into water purification into glass jar and controlling
Inflow processed；Discharging device includes drainage hole, overflow pipe and drainpipe, and the discharging device is the setting of upper and lower vertical direction, is used
In cultivation water in discharge glass jar, and then the pollutants such as residual bait, excrement is made to be discharged with water (flow) direction or collect convenient for concentration soil pick-up
Processing.And Chinese patent application application number 201510486547.1, a kind of shrimp culture tail water circulation process method is disclosed, including
Sieve is set above the filtering ponds in shrimp culture canopy downstream, is precipitated in sedimentation basin by the cultivation tail water of sieve, by heavy
Cultivation tail water behind shallow lake introduces stream and breeding water is led back to cultivation reservoir again by microorganism in stream and plant purification.
Though above-mentioned related patents and related document have the report of the step of disinfection, miniflow water cleaning and method, about shrimp without section children
Being related to for body is very few.
Summary of the invention
In view of the deficiencies of the prior art, the present invention provides a kind of device applied to shrimp nauplius miniflow water cleaning and sterilizing
And method, when can be for nauplius oviposition and residue when development of fertilized ova, and due to the growth characteristics of nauplius,
Some microorganisms for influencing larval culture are unavoidably carried in temporary rearing process, are effectively removed and reduced content.
The technical solution of the present invention is as follows:
A kind of device applied to shrimp nauplius miniflow water cleaning and sterilizing, it is characterised in that: described device includes temporarily supporting
Bucket and circular cylinder cage, the circular cylinder cage are placed in temporarily feeding bucket and are higher by the temporarily top of feeding bucket along 20cm, avoid the young
It is run in fluvial processes and leaks out the young temporarily feeding bucket；The circular cylinder cage includes support frame, the cylindrical string bag and lashing, described
Support frame is made of stainless steel tube welding, is equipped with lashing, the cylinder string bag along with bottom on the cylinder string bag
It is fixed by lashing and support frame, fits closely the entire cylindrical string bag with support frame, form a complete cylinder
Shape net cage；The circular cylinder cage central location is equipped with tracheae and gas exhausting stone, and the tracheae is vertically arranged, the tracheae and air-dispersing
Stone penetrates a lead button when connecting, and prevents gas exhausting stone from swinging everywhere when inflating；The tracheae is transparent hose, is set on the tracheae
There is air valve switch, air valve switch is used to control the size of tolerance；It is additionally provided with tap right above the circular cylinder cage center, institute
It states and a digital indication flow meter is installed in front of tap, be used to indicate water yield, it is ensured that gas exhausting stone connects just with net cage bottom
Touching；The tracheae is hung on tap.
Further, the temporarily feeding bucket is cylinder, and volume is 0.5~1.0m3, it is highly 0.8~1.0m, this temporarily supports bucket
10,000,000 tail of nauplius~30,000,000 tails can be accommodated.
Further, the aperture of the cylindrical string bag is 200 mesh.
The present invention also protects a kind of method applied to shrimp nauplius miniflow water cleaning and sterilizing, which is characterized in that the party
Method the following steps are included:
Step 1: the device of production miniflow water cleaning and sterilizing；
Step 2: it micro- discharge condition: is tested by multiple flowing water and to vibrios in nauplius vibrios carrying amount, water body
The tracking of content obtains most suitable micro- flow time and uninterrupted；
Step 3: the detection of content of microorganisms: content of microorganisms and nauplius sheet including detecting water in temporarily feeding bucket
The content of microorganisms that body carries, the former carries out the coating of tcbs plate by water sampling, and the latter carries out after taking nauplius to be fully ground
The coating of tcbs plate, then cultivates 16~18h in constant incubator；
Step 4: it the damage ratio of nauplius and sticks the detection of dirty rate: carrying out microscopy with electron microscope, damage ratio is mainly seen
Examining nauplius, whether there is or not there are tail spine, appendage, perhaps bristle fracture or presence are squeezed and dead individual；Stick dirty rate master
It observes nauplius body or whether appendage bristle is sticked together by the organic substance in water.
Further, every water quality indicator of miniflow water must be consistent with the water in hatching larvae pond in step 2,
Including temperature, salinity, total alkalinity and pH value；It is tested by multiple flowing water and to arc in nauplius vibrios carrying amount, water body
The tracking of bacterial content show that the optimal cleaning flow of miniflow water is 0.6~1.0m3/ h, 2~3h of scavenging period.
Further, the specific steps of the detection of content of microorganisms described in step 3 include: clear in progress miniflow water respectively
Before and after wash disinfection, the water sample in temporarily feeding bucket is taken to carry out the coating of tcbs plate to superclean bench；Carrying out the cleaning of miniflow water respectively
Disinfection front and back takes the nauplius in temporarily feeding bucket, connects the young with the dredger disinfected, spray 75% alcohol washes, then spray
Distilled water, precision weighing 0.1g are sufficiently mixed grinding with the physiological saline of 400uL 0.85%, choose in superclean bench
100uL sample adds to tcbs plate, is sufficiently applied with spreading rod uniformly, after being cultivated 16~18 hours in 31 DEG C of insulating boxs, observation
Bacterium colony growing state in plate.
Further, the damage ratio of step 4 nauplius and to stick the specific steps of dirty rate detection include: to use electron microscopic
Mirror microscopy, is observed under amplification factor 20~40, and the tail spine, appendage or the bristle that show as nauplius of damage have fracture feelings
Condition, or exist and be squeezed and the dead young；The dirty body for showing as nauplius or appendage bristle are sticked by water
Organic matter sticks together；No less than 100 tail nauplius microscopies are taken respectively, and the young of damage is counted with the dirty young is sticked respectively, system
It counts out the damage ratio of the batch young and sticks dirty rate.
The invention has the benefit that apparatus of the present invention structure is simple, low manufacture cost, operating method is simple and convenient, and
Positive effect can be reached.By being slowly injected into new water, row changes the old water in temporarily feeding bucket in time, by the characteristic of mixed solution,
To achieve the effect that the microorganism and the residuals content that reduce unit of water body, nauplius is carried out with the method for the present invention micro-
Flowing water disinfection, the young is not damaged, will not generate stress, compared to traditional nauplius cleaning method, miniflow of the present invention
The method of water cleaning and sterilizing can make the damage ratio of the young and stick dirty rate reduction by 20%~40%, and the vibrios carrying amount of the young reduces
50%~80%, it is very suitable to the utilization of nauplius actual production, to optimize the quality of nauplius, effectively facilitates children
The survival rate of seedling of body.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of apparatus of the present invention；
Fig. 2 is the structural schematic diagram of circular cylinder cage of the present invention.
In figure, 1, temporarily support bucket；2, circular cylinder cage；21, support frame；22, the cylindrical string bag；23, lashing.
Specific embodiment
Specific embodiments of the present invention will be further explained with reference to the accompanying drawing:
Embodiment 1:
As shown in Figs. 1-2, a method of applied to shrimp nauplius miniflow water cleaning and sterilizing, comprising the following steps:
(1) make the device of miniflow water cleaning and sterilizing: described device includes temporarily supporting bucket 1 and circular cylinder cage 2, the cylinder
Shape net cage 2 is placed in temporarily feeding bucket 1 and is higher by the temporarily top of feeding bucket 1 along 20cm, and the circular cylinder cage 2 includes support frame
21, the cylindrical string bag 22 and lashing 23, the support frame 21 are made of stainless steel tube welding, edge on the cylinder string bag 22
It is equipped with lashing 23 with bottom, the cylinder string bag 22 is fixed by lashing 23 and support frame 21, makes entire cylindrical net
Pocket 22 is fitted closely with support frame 21, forms a complete circular cylinder cage 2；2 central location of circular cylinder cage is set
There are tracheae and gas exhausting stone (not shown), the tracheae is vertically arranged, and the tracheae penetrates a lead button, institute when connecting with gas exhausting stone
Stating tracheae is transparent hose, and the tracheae is equipped with air valve switch, is additionally provided with tap right above 2 center of circular cylinder cage
(not shown), the tap front are equipped with a digital indication flow meter, and the tracheae is hung on tap；Wherein, described
Temporarily feeding bucket 1 is cylinder, volume 0.5m3, it is highly 0.8m, 15,000,000 tail of nauplius, the cylinder net can be accommodated
The aperture of pocket 22 is 200 mesh；
(2) micro- discharge condition: Penaeus Vannmei nauplius is placed in circular cylinder cage, and every water quality of miniflow water refers to
Mark must be consistent with the water in hatching larvae pond, wherein 30.5 DEG C of temperature, salinity 32 ‰, total alkalinity 120 and pH value 8.2,
The cleaning flow that miniflow water is arranged is 0.6m3/ h, scavenging period 2h；
It (3) include that the content of microorganisms that the content of microorganisms of water and nauplius itself carry in bucket is temporarily supported in detection, point
Not before and after carrying out miniflow water cleaning and sterilizing, the water sample in temporarily feeding bucket is taken to carry out the coating of tcbs plate to superclean bench, specifically
Operation is that 100uL water sample stoste is taken to be added in plate, is sufficiently applied with spreading rod uniformly；Carrying out miniflow water cleaning and sterilizing respectively
Front and back takes the nauplius in temporarily feeding bucket, connects the young with the dredger disinfected, spray 75% alcohol washes, then steam and evaporate
Water, precision weighing 0.1g are sufficiently mixed grinding with the physiological saline of 400uL 0.85%, 100uL are chosen in superclean bench
Sample adds to tcbs plate, is sufficiently applied with spreading rod uniformly, after cultivating 16 hours in 31 DEG C of insulating boxs, observes bacterium colony in plate
Growing state；
(4) damage ratio of nauplius and the detection of dirty rate is sticked: with electronics sediments microscope inspection, the observation under amplification factor 25,
Perhaps bristle has crack conditions or presence to be squeezed and dead children for the tail spine for showing as nauplius of damage, appendage
Body；The dirty body for showing as nauplius or appendage bristle is sticked to be sticked together by the organic matter in water；Respectively to 100 tail nauplius
Microscopy respectively counts the young of damage with the dirty young is sticked, counts the damage ratio of the batch Penaeus Vannmei nauplius
And dirty rate is sticked, carry out data record.The results are shown in Table 1 for record.
Contrast on effect before and after the nauplius miniflow water method for cleaning and disinfecting of the present invention of table 1
Seen from table 1, by the comparison of effect before and after miniflow water cleaning and sterilizing, water vibrios content and the south in bucket are temporarily supported
The self-contained vibrios content of whitening nauplius substantially reduces, and furthermore does not cause to damage to the young, while can also be effectively reduced
The young sticks dirty rate, it may be said that can increase substantially the quality of nauplius by the method for the invention, reduce Penaeus Vannmei and educate
The occurrence probability of pest and disease damage during seedling improves the quality and economic benefit of seedling.
Embodiment 2:
As shown in Figs. 1-2, a method of applied to shrimp nauplius miniflow water cleaning and sterilizing, comprising the following steps:
(1) make the device of miniflow water cleaning and sterilizing: described device includes temporarily supporting bucket 1 and circular cylinder cage 2, the cylinder
Shape net cage 2 is placed in temporarily feeding bucket 1 and is higher by the temporarily top of feeding bucket 1 along 20cm, and the circular cylinder cage 2 includes support frame
21, the cylindrical string bag 22 and lashing 23, the support frame 21 are made of stainless steel tube welding, edge on the cylinder string bag 22
It is equipped with lashing 23 with bottom, the cylinder string bag 22 is fixed by lashing 23 and support frame 21, makes entire cylindrical net
Pocket 22 is fitted closely with support frame 21, forms a complete circular cylinder cage 2；2 central location of circular cylinder cage is set
There are tracheae and gas exhausting stone, the tracheae is vertically arranged, and the tracheae penetrates a lead button when connecting with gas exhausting stone, and the tracheae is
Transparent hose, the tracheae are equipped with air valve switch, are additionally provided with tap, the water right above 2 center of circular cylinder cage
Leading front is equipped with a digital indication flow meter, and the tracheae is hung on tap；Wherein, the temporarily feeding bucket 1 is cylinder,
Volume is 1.0m3, it is highly 1.0m, 30,000,000 tail of nauplius can be accommodated, the aperture of the cylinder string bag 22 is 200
Mesh；
(2) micro- discharge condition: Penaeus Vannmei nauplius is placed in circular cylinder cage, and every water quality of miniflow water refers to
Mark must be consistent with the water in hatching larvae pond, including 31 DEG C of temperature, salinity 31 ‰, total alkalinity 130 and pH value 8.0, if
The cleaning flow for setting miniflow water is 0.8m3/ h, scavenging period 2h；
(3) detection of content of microorganisms: a, the operation to miniflow water cleaning and sterilizing is used detect micro- life of water in temporarily feeding bucket
The content of microorganisms that object content and nauplius itself carry takes in temporarily feeding bucket after carrying out miniflow water cleaning and sterilizing 2h
Water sample takes 100uL water sample stoste to be added in tcbs plate to superclean bench, is sufficiently applied with spreading rod uniformly；It takes and temporarily supports again
Nauplius in bucket, connects the young with the dredger disinfected, sprays 75% alcohol washes, then steam distilled water, precision weighing
0.1g is sufficiently mixed grinding with the physiological saline of 400uL 0.85%, and 100uL sample is chosen in superclean bench and adds to tcbs
Plate is sufficiently applied uniformly with spreading rod, after cultivating 18 hours in 31 DEG C of insulating boxs, observes bacterium colony growing state in plate；b,
To the operation using conventional method, after change water to process, sampling and the same a of detection method；
(4) damage ratio of nauplius and the detection of dirty rate is sticked: with electronics sediments microscope inspection, the observation under amplification factor 20,
Perhaps bristle has crack conditions or presence to be squeezed and dead children for the tail spine for showing as nauplius of damage, appendage
Body；The dirty body for showing as nauplius or appendage bristle is sticked to be sticked together by the organic matter in water；Respectively to two kinds of operating methods
Treated 150 tail nauplius microscopies to the young of damage and stick the dirty young and count, it is white to count the batch South America respectively
The damage ratio of prawn nauplius and dirty rate is sticked, carries out data record.The results are shown in Table 2 for record.
The Contrast on effect of table 2 nauplius miniflow water method for cleaning and disinfecting and conventional method of the present invention
As can be seen from Table 2, passing through the Contrast on effect of miniflow water method for cleaning and disinfecting and conventional method, the water in bucket is temporarily supported
Great variety all has occurred in body, nauplius vibrios content, and conventional method is to young damage ratio and the raising for sticking dirty rate
There is certain facilitation, it can be seen that, nauplius vibrios content can be effectively reduced by the method for miniflow water cleaning and sterilizing,
That reduces the young sticks dirty rate, not will cause the damage of the young.
(5) nursery nauplius development tracking: is transported to the Penaeus Vannmei nauplius by two methods processing
, it is launched in same workshop, in the case where all grow seedling links, personnel's operation and environmental factor are consistent, warp
The nauplius that miniflow water cleaning and sterilizing is crossed physically well develops in Miao Chi, ingest preferably, drag just more, normal metamorphosis, waste is few；
And exist for the paedomorphosis through conventional method and hang dirty phenomenon, it ingests and drags just generally, the abnormal time is clearly present
Lag, and waste is more.
Embodiment 3:
As shown in Figs. 1-2, a method of applied to shrimp nauplius miniflow water cleaning and sterilizing, comprising the following steps:
(1) make the device of miniflow water cleaning and sterilizing: described device includes temporarily supporting bucket 1 and circular cylinder cage 2, the cylinder
Shape net cage 2 is placed in temporarily feeding bucket 1 and is higher by the temporarily top of feeding bucket 1 along 20cm, and the circular cylinder cage 2 includes support frame
21, the cylindrical string bag 22 and lashing 23, the support frame 21 are made of stainless steel tube welding, edge on the cylinder string bag 22
It is equipped with lashing 23 with bottom, the cylinder string bag 22 is fixed by lashing 23 and support frame 21, makes entire cylindrical net
Pocket 22 is fitted closely with support frame 21, forms a complete circular cylinder cage 2；2 central location of circular cylinder cage is set
There are tracheae and gas exhausting stone, the tracheae is vertically arranged, and the tracheae penetrates a lead button when connecting with gas exhausting stone, and the tracheae is
Transparent hose, the tracheae are equipped with air valve switch, are additionally provided with tap, the water right above 2 center of circular cylinder cage
Leading front is equipped with a digital indication flow meter, and the tracheae is hung on tap；Wherein, the temporarily feeding bucket 1 is cylinder,
Volume is 1.0m3, it is highly 0.8m, 30,000,000 tail of nauplius can be accommodated, the aperture of the cylinder string bag 22 is 200
Mesh；
(2) micro- discharge condition: Penaeus monodon nauplius is placed in circular cylinder cage, every water quality indicator of miniflow water
It must be consistent with the water in hatching larvae pond, including 29.5 DEG C of temperature, salinity 30 ‰, total alkalinity 120 and pH value 8.0, if
The cleaning flow for setting miniflow water is 0.6m3/ h, scavenging period 2.5h；
(3) detection of content of microorganisms: detection temporarily supports what the content of microorganisms of water and nauplius itself in bucket carried
Content of microorganisms takes the water sample in temporarily feeding bucket to superclean bench, takes 100uL water after carrying out miniflow water cleaning and sterilizing 2.5h
Sample stoste is added in tcbs plate, is sufficiently applied with spreading rod uniformly；Meanwhile the Penaeus monodon nauplius temporarily supported in bucket is taken,
The young is connect with the dredger disinfected, sprays 75% alcohol washes, then steams distilled water, precision weighing 0.1g, with 400uL
0.85% physiological saline is sufficiently mixed grinding, and 100uL sample is chosen in superclean bench and adds to tcbs plate, uses spreading rod
It sufficiently applies uniformly, after cultivating 16 hours in 31 DEG C of insulating boxs, observes bacterium colony growing state in plate；
(4) damage ratio of Penaeus monodon nauplius and the detection of dirty rate is sticked: with electronics sediments microscope inspection, in amplification factor 25
Lower observation, perhaps bristle has crack conditions or dead in the presence of being squeezed for the tail spine for showing as nauplius of damage, appendage
The young died；The dirty body for showing as nauplius or appendage bristle is sticked to be sticked together by the organic matter in water；Take respectively 100 tails without
Young microscopy is saved, the young of damage is counted with the dirty young is sticked respectively, the damage ratio of the batch young is counted and sticks dirty rate,
Carry out data record；
(5) the Penaeus monodon nauplius situation after miniflow water cleaning and sterilizing: vibrio flavus bacterium colony and green arc in water body
Bacterium Microflora is 20cfu/ml and 3cfu/ml respectively, the self-contained vibrio flavus bacterium colony of the young and green vibrios quantity
It is 0cfu/ml and 0cfu/ml respectively, young damage ratio and to stick dirty rate be 4% and 0% respectively；
(6) Penaeus monodon nauplius nursery effect: the batch nauplius after miniflow water cleaning and sterilizing, it is entire to send out
The process of educating is acted normally, and the phenomenon that development lag does not occur, and overall survival rate of seedling reaches 70%, is much higher than other spot sections pair
Shrimp 38% is averaged survival rate of seedling.
Embodiment 4:
As shown in Figs. 1-2, a method of applied to shrimp nauplius miniflow water cleaning and sterilizing, comprising the following steps:
(1) make the device of miniflow water cleaning and sterilizing: described device includes temporarily supporting bucket 1 and circular cylinder cage 2, the cylinder
Shape net cage 2 is placed in temporarily feeding bucket 1 and is higher by the temporarily top of feeding bucket 1 along 20cm, and the circular cylinder cage 2 includes support frame
21, the cylindrical string bag 22 and lashing 23, the support frame 21 are made of stainless steel tube welding, edge on the cylinder string bag 22
It is equipped with lashing 23 with bottom, the cylinder string bag 22 is fixed by lashing 23 and support frame 21, makes entire cylindrical net
Pocket 22 is fitted closely with support frame 21, forms a complete circular cylinder cage 2；2 central location of circular cylinder cage is set
There are tracheae and gas exhausting stone, the tracheae is vertically arranged, and the tracheae penetrates a lead button when connecting with gas exhausting stone, and the tracheae is
Transparent hose, the tracheae are equipped with air valve switch, are additionally provided with tap, the water right above 2 center of circular cylinder cage
Leading front is equipped with a digital indication flow meter, and the tracheae is hung on tap；Wherein, the temporarily feeding bucket 1 is cylinder,
Volume is 1.0m3, it is highly 0.8m, 25,000,000 tail of nauplius can be accommodated, the aperture of the cylinder string bag 22 is 200
Mesh；
(2) micro- discharge condition: Penaeus monodon nauplius is placed in circular cylinder cage, every water quality indicator of miniflow water
It must be consistent, including 31 DEG C of temperature, salinity 31 ‰, total alkalinity 120 and pH value 8.15, be arranged with the water in hatching larvae pond
The cleaning flow of miniflow water is 1.0m3/ h, scavenging period 3h；
(3) detection of content of microorganisms:
A, to the operation using miniflow water cleaning and sterilizing, the content of microorganisms and nauplius sheet of water in temporarily feeding bucket are detected
The content of microorganisms that body carries takes the water sample in temporarily feeding bucket to superclean bench, takes after carrying out miniflow water cleaning and sterilizing 3h
100uL water sample stoste is added in tcbs plate, is sufficiently applied with spreading rod uniformly；The nauplius in temporarily feeding bucket is taken again, with
The dredger disinfected connects the young, sprays 75% alcohol washes, then steams distilled water, precision weighing 0.1g, with 400uL 0.85%
Physiological saline be sufficiently mixed grinding, in superclean bench choose 100uL sample add to tcbs plate, sufficiently applied with spreading rod
Uniformly, after cultivating 18 hours in 31 DEG C of insulating boxs, bacterium colony growing state in plate is observed；
B, to the operation using conventional method, after change water to process, sampling and the same a of detection method；
(4) damage ratio of nauplius and the detection of dirty rate is sticked: with electronics sediments microscope inspection, the observation under amplification factor 25,
Perhaps bristle has crack conditions or presence to be squeezed and dead children for the tail spine for showing as nauplius of damage, appendage
Body；The dirty body for showing as nauplius or appendage bristle is sticked to be sticked together by the organic matter in water；Respectively to two kinds of operating methods
Treated 100 tail nauplius microscopies to the young of damage and stick the dirty young and count, count the batch spot section pair respectively
The damage ratio of shrimp nauplius and dirty rate is sticked, carries out data record.The results are shown in Table 3 for record.
The Contrast on effect of table 3 nauplius miniflow water method for cleaning and disinfecting and conventional method of the present invention
Seen from table 3, for Penaeus monodon nauplius, after being handled by miniflow water method for cleaning and disinfecting, compared to biography
System operating method, temporarily the water body in feeding bucket, nauplius vibrios content significantly reduce, and do not impact, go back to young damage
The young can be promoted, which to stick dirty rate, to reduce, it can be seen that, Penaeus monodon can effectively be optimized without section by the method for miniflow water cleaning and sterilizing
The quality of the young, and then the survival rate of nursery is improved, improve the quality of seedling.
The above embodiments and description only illustrate the principle of the present invention and most preferred embodiment, is not departing from this
Under the premise of spirit and range, various changes and improvements may be made to the invention, these changes and improvements both fall within requirement and protect
In the scope of the invention of shield.
Claims (7)
1. a kind of device applied to shrimp nauplius miniflow water cleaning and sterilizing, it is characterised in that: described device includes temporarily supporting bucket
And circular cylinder cage, the circular cylinder cage are placed in temporarily feeding bucket and are higher by the temporarily top of feeding bucket along 20cm, the cylinder
Net cage includes that support frame, the cylindrical string bag and lashing, the support frame are made of stainless steel tube welding, the cylinder net
It is equipped with lashing along with bottom on pocket, the cylinder string bag is fixed by lashing and support frame, makes the entire cylindrical string bag
It is fitted closely with support frame, forms a complete circular cylinder cage；The circular cylinder cage central location be equipped with tracheae and
Gas exhausting stone, the tracheae are vertically arranged, and the tracheae penetrates a lead button when connecting with gas exhausting stone, and the tracheae is transparent soft
Pipe, the tracheae are equipped with air valve switch, and tap, the tap front are additionally provided with right above the circular cylinder cage center
One digital indication flow meter is installed, the tracheae is hung on tap.
2. a kind of device applied to shrimp nauplius miniflow water cleaning and sterilizing according to claim 1, it is characterised in that:
The temporarily feeding bucket is cylinder, and volume is 0.5~1.0m3, it is highly 0.8~1.0m.
3. a kind of device applied to shrimp nauplius miniflow water cleaning and sterilizing according to claim 1, it is characterised in that:
The aperture of the cylinder string bag is 200 mesh.
4. a kind of method applied to shrimp nauplius miniflow water cleaning and sterilizing, which is characterized in that method includes the following steps:
Step 1: the device of production miniflow water cleaning and sterilizing；
Step 2: it micro- discharge condition: is tested by multiple flowing water and to vibrios content in nauplius vibrios carrying amount, water body
Tracking, obtain most suitable micro- flow time and uninterrupted；
Step 3: it the detection of content of microorganisms: is taken including detecting the content of microorganisms of water and nauplius itself in temporarily feeding bucket
The content of microorganisms of band, the former water sampling carry out the coating of tcbs plate, and it is flat that the latter carries out tcbs after taking nauplius to be fully ground
Plate coating, then cultivates 16~18h in constant incubator；
Step 4: the damage ratio of nauplius and stick the detection of dirty rate: carrying out microscopy with electron microscope, damage ratio main detection without
Whether there is or not there are tail spine, appendage, perhaps bristle fracture or presence are squeezed and dead individual for the section young；Dirty rate is sticked mainly to see
It examines nauplius body or whether appendage bristle is sticked together by the organic substance in water.
5. a kind of method applied to shrimp nauplius miniflow water cleaning and sterilizing according to claim 4, it is characterised in that:
Every water quality indicator of miniflow water must be consistent with the water in hatching larvae pond in step 2, including temperature, salinity, total alkali
Degree and pH value；It is tested and the tracking to vibrios content in nauplius vibrios carrying amount, water body, is obtained micro- by multiple flowing water
The optimal cleaning flow of flowing water is 0.6~1.0m3/ h, 2~3h of scavenging period.
6. a kind of method applied to shrimp nauplius miniflow water cleaning and sterilizing according to claim 4, it is characterised in that:
The specific steps of the detection of content of microorganisms described in step 3 include: to take and temporarily support respectively before and after carrying out miniflow water cleaning and sterilizing
Water sample in bucket carries out the coating of tcbs plate to superclean bench；Respectively before and after carrying out miniflow water cleaning and sterilizing, temporarily feeding bucket is taken
In nauplius, connect the young with the dredger disinfected, spray 75% alcohol washes, then steam distilled water, precision weighing
0.1g is sufficiently mixed grinding with the physiological saline of 400uL0.85%, and 100uL sample is chosen in superclean bench and adds to tcbs
Plate is sufficiently applied uniformly with spreading rod, after cultivating 16~18 hours in 31 DEG C of insulating boxs, is observed bacterium colony in plate and is grown feelings
Condition.
7. a kind of method applied to shrimp nauplius miniflow water cleaning and sterilizing according to claim 4, it is characterised in that:
The damage ratio of step 4 nauplius and the specific steps for sticking the detection of dirty rate include: with electronics sediments microscope inspection, in amplification factor
20~40 lower observations, perhaps bristle has crack conditions or exists by crowded for the tail spine for showing as nauplius of damage, appendage
It presses and the young of death；The dirty body for showing as nauplius or appendage bristle is sticked to be sticked together by the organic matter in water；It takes respectively
No less than 100 tail nauplius microscopies respectively count the young of damage with the dirty young is sticked, count the damage of the batch young
Hurt rate and sticks dirty rate.
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| CN201811498611.8A CN109644909A (en) | 2018-12-08 | 2018-12-08 | A kind of device and method applied to shrimp nauplius miniflow water cleaning and sterilizing |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112646786A (en) * | 2021-01-21 | 2021-04-13 | 海南海壹水产种苗有限公司 | Rapid preliminary separation method for vibrio kammaticus phage |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106719200A (en) * | 2016-12-15 | 2017-05-31 | 雷州市海威水产养殖有限公司 | A kind of Penaeus Vannmei nauplius method for optimizing |
| CN108094272A (en) * | 2017-12-31 | 2018-06-01 | 连云港市海洋与渔业发展促进中心 | Efficient bait-throwing method in a kind of cultivating pool |
| CN108094283A (en) * | 2017-12-29 | 2018-06-01 | 海南中正水产科技有限公司 | A kind of method of indoor industrially biological breeding Penaeus Vannmei shrimp seedling |
-
2018
- 2018-12-08 CN CN201811498611.8A patent/CN109644909A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106719200A (en) * | 2016-12-15 | 2017-05-31 | 雷州市海威水产养殖有限公司 | A kind of Penaeus Vannmei nauplius method for optimizing |
| CN108094283A (en) * | 2017-12-29 | 2018-06-01 | 海南中正水产科技有限公司 | A kind of method of indoor industrially biological breeding Penaeus Vannmei shrimp seedling |
| CN108094272A (en) * | 2017-12-31 | 2018-06-01 | 连云港市海洋与渔业发展促进中心 | Efficient bait-throwing method in a kind of cultivating pool |
Non-Patent Citations (2)
| Title |
|---|
| 孔巧香: "南美白对虾主要致病微生物安全预警技术研究", 《中国优秀硕士学位论文全文数据库农业科技辑》 * |
| 梁东列: "选虾苗——先了解育苗技术", 《中国渔业报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112646786A (en) * | 2021-01-21 | 2021-04-13 | 海南海壹水产种苗有限公司 | Rapid preliminary separation method for vibrio kammaticus phage |
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Application publication date: 20190419 |