CN102487898B - Culture method and culture device used before attachment of coral oosperms - Google Patents
Culture method and culture device used before attachment of coral oosperms Download PDFInfo
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- CN102487898B CN102487898B CN201110393700.8A CN201110393700A CN102487898B CN 102487898 B CN102487898 B CN 102487898B CN 201110393700 A CN201110393700 A CN 201110393700A CN 102487898 B CN102487898 B CN 102487898B
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Abstract
The invention discloses a culture method and a culture device used before attachment of coral oosperms. A plurality of culture round tubes are arranged in a seawater container; one end of each culture round tube is sealed by a filter screen, and the other end of each culture round tube is provided with an opening; each culture round tube is placed in the seawater container in a manner that the filter screen end of each culture round tube is downward and the opening end of each culture round tube is upward; fresh and clean seawater is added into the seawater container, and the water temperature of the seawater is maintained at 27-28DEG C, so that the filter screen end of each culture round tube is in the seawater, and the opening end of each culture round tube is exposed to the water surface, so that the culture water surface area in each culture round tube is 176-314cm<2>; the coral oosperms are poured into the culture round tubes along with the seawater, wherein the number of the coral oosperms in each culture round tube is 1,000-1,500; the seawater in the culture round tubes and the seawater container is frequently replaced so as to maintain the cleanness of the culture environment; and if dead and albescent individuals of the coral oosperms exist, the dead and albescent individuals are sucked out, and culture based on such a method is carried out until the coral oosperms develops to planula. By utilizing the method and device disclosed by the invention to culture the coral oosperms, the survival rate of the coral oosperms can be greatly improved.
Description
Technical field:
The invention belongs to coral cultivation and coral reef and manually repair field, be specifically related to a kind of coral fertilized egg and adhere to front breeding method and breeding arrangement.
Background technology:
Coral is being subject to more and more serious threat over nearest 50 years, and under the mankind's impact and the dual-pressure of global warming, quantity reduces rapidly, has occurred in the world the phenomenon that many places coral reef is degenerated.The islands and reefs that the minimizing of coral forms coral reef and coral reef are subject to the erosion of wave and storm and destroy and cannot repair, and coral reef and islands and reefs can dwindle disappearance gradually under corroding.In addition, the biomass of living in coral reef also can significantly lower, and bio-diversity reduces, and this has also affected the quantity of the catch of fishery and fisherman's income.In order to slow down the rate of decay of coral quantity, increase coral quantity, maintain bio-diversity in coral reef, cultivating coral and again cultivating them is a kind of positive manual reversion means that we can adopt at present to impaired coral reef.The cultivation of coral has comprised sexual and asexual two kinds of methods, and majority is all the method for utilizing asexual reproduction, but this method need to gather coral parent for transplanting from coral reef.Sexual propagation is to utilize the ovum of coral discharge, is allowed to condition at experiment growth in the laboratory, adheres to, grows to little corallite, then migrates to field, and this method has just reduced the needs to field coral parent.
But in the process of coral development of fertilized ova, how fragile coral ovum being cultivated into freely movable Metamorphore is one of wherein crucial step.Coral ovum belongs to buoyant egg, easily on the water surface, assembles agglomeratingly, and is easily attached on the impurity of casing wall or the water surface, causes the ovum can not normal development or death, and dead ovum also easily causes the ovum of periphery to be infected by fungi or bacterium after rotting.In addition be gathered into large stretch of coral ovum and cover the exchange of having blocked water body and air on the water surface, easily cause the coral ovum death by suffocation in bottom.How to control fertilized egg and at the water surface, do not assemble agglomeratingly, and reduce dead coral ovum and the impact of other ovum is become to the key of whole cultivating process.
External coral ovum type of rearing utilizes the semi-open container of filter screen isolation to cultivate coral ovum for directly cultivating in culturing jar or tank, having no.Domestic also without relevant coral ovum cultivation record.
Summary of the invention:
The object of this invention is to provide and a kind ofly can alleviate coral fertilized egg and in growth course, easily stick agglomerate, and reduce the phenomenons such as impact of dead coral ovum on water quality, thereby improve breeding method and breeding arrangement before coral development of fertilized ova to the coral fertilized egg of the survival rate in Metamorphore stage is adhered to.
Breeding method before coral fertilized egg of the present invention is adhered to, is characterized in that, comprises the following steps:
Several are set in containing sea container and cultivate pipe, described cultivation pipe one end seals with filter screen, in case coral fertilized egg is flowed out, an other end opening, cultivates the filter screen end of pipe down, openend is positioned over upward to be contained in sea container, toward containing sea container, add fresh clean seawater again, and to keep seawater water temperature be 27～28 ℃, make the filter screen end of cultivating pipe in seawater, and openend is exposed on the water surface, the cultivation water surface area that each is cultivated in pipe is 176～314cm
2coral fertilized egg is poured into and cultivated in pipe with seawater, each coral fertilized egg number of cultivating in pipe is 1000～1500, often change and cultivate pipe and contain the seawater in sea container, to keep the cleaning of nurturing an environment, if the individuality that coral fertilized egg has death to bleach, in time sucking-off is removed, so cultivate until coral development of fertilized ova to Metamorphore.
Metamorphore can be for the attached cultivation coral young.
The seawater that described frequent replacing is cultivated in pipe and Sheng sea container preferably adopts circulating seawer system, to keep the cleaning of seawater, is conducive to the hatching of coral fertilized egg.Seawater will be selected fresh clean seawater, avoid collecting seawater in the marine site of being polluted.The screen filtration of the most handy aperture 20 μ m of seawater that collect, if can filter with the filter membrane of 0.2 μ m better effects if.
It is highly Plastic Drum or the Glass fibre reinforced plastic tub that 50～60cm is high that described Sheng sea container is preferably, it is 15～20cm that described cultivation pipe is preferably internal diameter, length is the plastic tube of 20～30cm, the aperture of described filter screen is preferably 120～200 μ m, in case coral fertilized egg is flowed out and is cultivated pipe with current, add and contain the sea water advanced 30～35cm of being of sea container, again plastic tube is hung on to two ends frame on the cross bar of containing on sea container with fishing line or iron wire, make the mouth of pipe higher than the water surface 5～10cm, each seawater of cultivating in pipe was at least changed once in each hour, the seawater of containing in sea container is changed 2 times every day, each seawater of 50% of changing.
Described coral fertilized egg is preferably the ovulation type corals such as Acropora and rose coral and discharges the fertilized egg in latter 1～2 hour.
In order to adapt to coral fertilized egg of the present invention, adhere to front breeding method, the inventor has also designed breeding arrangement, it is characterized in that, comprise and contain sea container and cultivate pipe, described cultivation pipe one end seals with filter screen, and an other end opening is cultivated pipe filter screen end down, openend is positioned over upward to be contained in sea container, and each cross section area of pipe of cultivating pipe is 176～314cm
2.。
It is highly Plastic Drum or the Glass fibre reinforced plastic tub that 50～60cm is high that described Sheng sea container is preferably, it is 15～20cm that described cultivation pipe is preferably internal diameter, length is the plastic tube of 20～30cm, the aperture of described filter screen is preferably 120～200 μ m, described plastic tube hangs on two ends frame on the cross bar of containing on sea container with fishing line or steel wire, at each, cultivate in pipe and be provided with feed pipe, feed pipe is connected with water pump.
By processing of the present invention, coral fertilized egg is difficult for assembling on the water surface agglomerating, is difficult for adhering to tube wall, and therefore fresh clean seawater free from admixture can not stick on impurity yet.After coral fertilized egg being cultivated by method of the present invention, the survival rate of coral fertilized egg improves greatly, than being simply positioned over the coral fertilized egg survival rate of cultivating in water vat less than 1%, the survival rate of coral fertilized egg of the present invention is 13.6%, and has reduced the impact of dead coral on water quality.
Accompanying drawing explanation:
Fig. 1 is the structural representation of breeding arrangement of the present invention;
Wherein 1, Plastic Drum; 2, plastic tube; 3, fishing line; 4, the first cross bar; 5, the second cross bar; 6, feed pipe; 7, water pump; 71, the water inlet pipe of water pump.
Embodiment:
Following examples are to further illustrate of the present invention, rather than limitation of the present invention.
Embodiment 1:
As shown in Figure 1, Plastic Drum 1 and the internal diameter of the breeding arrangement Bao Gaowei 50～60cm of the present embodiment are 15～20cm, length is the plastic tube 2 of 20～30cm, with the filter screen of aperture 120～200 μ m, one end of plastic tube is sealed, filter screen with plastic tube 2 sealings closely, can not there be gap and fold, to prevent coral fertilized egg with seawater outflow plastic tube 2 or to plunge to death in fold.The disinfecting solution of potassium permanganate that all breeding arrangement will be 0.5% with mass fraction before use, and rinse well with fresh water, in Plastic Drum, add fresh clean seawater (will avoid collecting seawater in the marine site of being polluted.The screen filtration of the most handy aperture 20 μ m of seawater that collect, if can filter with the filter membrane of 0.2 μ m better effects if.) to 30～35cm height, and to keep ocean temperature be 27～28 ℃.On two relative limits of Plastic Drum, set up the first parallel cross bar 4 and the second cross bar 5, plastic tube 2 connects with fishing line 3 without one end of filter screen, with fishing line 3, hung on the first cross bar 4 and the second cross bar 5, and make plastic tube 2 mouths of pipe higher than the water surface 5～10cm, by the fertilized egg in after Acropora discharge 1～2 hour gently with seawater, pour into slowly in plastic tube, coral fertilized egg numerical control in each pipe is between 1000～1500, seawater in each plastic tube will at least be changed once in one hour, the present embodiment, replacing per hour once, the water pump 7 of available 20～50w enters the seawater in Plastic Drum 1 from water inlet pipe 71, feed pipe 6 through diameter 5mm pumps in plastic tube 2, notice that current must be low, flow-control is at 3～10ml/s, not directly use water impact coral fertilized egg.Seawater in Plastic Drum 1 is changed 2 times every day, changes 50% seawater at every turn.In cultivating process, if the individuality that the death of coral fertilized egg bleaches, want in time with suction pipe its careful sucking-off, avoid affecting the growth of other coral fertilized eggs, when coral fertilized egg major part is developed to Metamorphore, can remove the plastic tube filter screen at 2 ends, careful operation, avoid filter screen to attach the Metamorphore of coral, now the state of Metamorphore is basicly stable, can be for attached cultivation coral larva.
The inventor utilizes a plurality of above-mentioned breeding arrangement in April, 2010, wherein the height of the Plastic Drum in each breeding arrangement is between 50～60cm, the internal diameter of plastic tube is between 15～20cm, length is between 20～30cm, the aperture of filter screen is between 120～200 μ m, sea water advanced between 30～35cm in Plastic Drum, the plastics mouth of pipe is higher than between the water surface 5～10cm, according to the method described above the fertilized egg of the Acropora of collecting is cultivated again, approximately 70,000 coral ovum collecting are interspersed among in a plurality of plastic tubes in a plurality of Plastic Drums, coral fertilized egg in each plastic tube is 1000～1500, cultivate according to the method described above, after 4 days, cultivate into about 10,000 coral Metamorphores.Than in April, 2009, be positioned over simply the coral fertilized egg of cultivating in water vat by survival rate from bringing up to 13.6% less than 1%, and reduced the impact of dead coral ovum on water quality.
Claims (6)
1. a coral fertilized egg is adhered to front breeding method, it is characterized in that, comprise the following steps: several are set in containing sea container and cultivate pipe, described cultivation pipe one end seals with filter screen, an other end opening, cultivate the filter screen end of pipe down, openend is positioned over upward to be contained in sea container, toward containing sea container, add fresh clean seawater again, and to keep seawater water temperature be 27～28 ℃, make the filter screen end of cultivating pipe in seawater, and openend is exposed on the water surface, the cultivation water surface area that each is cultivated in pipe is 176～314cm
2coral fertilized egg is poured into and cultivated in pipe with seawater, each coral fertilized egg number of cultivating in pipe is 1000～1500, often change and cultivate pipe and contain the seawater in sea container, to keep the cleaning of nurturing an environment, if the individuality that coral fertilized egg has death to bleach, in time sucking-off is removed, so cultivate until coral development of fertilized ova to Metamorphore.
2. coral fertilized egg according to claim 1 is adhered to front breeding method, it is characterized in that, the seawater that described frequent replacing is cultivated in pipe and Sheng sea container is to adopt circulating seawer system.
3. coral fertilized egg according to claim 1 is adhered to front breeding method, it is characterized in that, described Sheng sea container is to be highly Plastic Drum or the Glass fibre reinforced plastic tub of 50～60cm, described cultivation pipe is that internal diameter is 15～20cm, length is the plastic tube of 20～30cm, the aperture of described filter screen is 120～200 μ m, add and contain the sea water advanced 30～35cm of being of sea container, again plastic tube is hung on to two ends frame on the cross bar of containing on sea container with fishing line or iron wire, make the mouth of pipe higher than the water surface 5～10cm, each seawater of cultivating in pipe was at least changed once in each hour, the seawater of containing in sea container is changed 2 times every day, each seawater of 50% of changing.
4. coral fertilized egg according to claim 1 is adhered to front breeding method, it is characterized in that, described coral fertilized egg is the fertilized egg in 1～2 hour after Acropora or the discharge of rose coral.
5. a coral fertilized egg breeding arrangement, it is characterized in that, comprise and contain sea container and cultivate pipe, described cultivation pipe one end seals with filter screen, an other end opening, cultivate pipe filter screen end down, openend is positioned over upward to be contained in sea container, and each cross section area of pipe of cultivating pipe is 176～314cm
2.
6. coral fertilized egg breeding arrangement according to claim 5, it is characterized in that, described Sheng sea container is to be highly Plastic Drum or the Glass fibre reinforced plastic tub of 50～60cm, described cultivation pipe is that internal diameter is 15～20cm, length is the plastic tube of 20～30cm, and the aperture of described filter screen is 120～200 μ m, and described plastic tube hangs on two ends frame on the cross bar of containing on sea container with fishing line or steel wire, at each, cultivate in pipe and be provided with feed pipe, feed pipe is connected with water pump.
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103583471B (en) * | 2013-11-11 | 2014-10-29 | 海南省海洋与渔业科学院 | Method for artificial rapid propagation expanding of succulent soft coral |
| CN112997922B (en) * | 2021-03-12 | 2022-10-04 | 长沙理工大学 | Coral oosperm collection device |
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Non-Patent Citations (3)
| Title |
|---|
| 气泡珊瑚的鉴别与饲养;James W.Fatherree;《水族世界》;20110430(第2期);40-47 * |
| 珊瑚的人工培育与移植复育;崔立勇;《齐鲁渔业》;20100630;第27卷(第6期);52-53 * |
| 珊瑚礁生态修复研究进展;李元超等;《生态学报》;20081031;第28卷(第10期);5047-5054 * |
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