CN109650546A - A kind of method of method and water purifying based on Daphnia magna removal water body cyanobacteria - Google Patents
A kind of method of method and water purifying based on Daphnia magna removal water body cyanobacteria Download PDFInfo
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- C02F3/00—Biological treatment of water, waste water, or sewage
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Abstract
The present invention relates to a kind of methods based on Daphnia magna removal water body cyanobacteria, for natural waters such as lake, reservoirs because receiving sanitary sewage, industrial wastewater etc. containing a large amount of nitrogen and phosphorus elements to cause Blue-green Algae Fast Growth problem, based on Daphnia magna and cyanobacteria food chain relation, develops and a kind of go to eliminate cyanobacteria in water as the biological method of principle using ecological system degradation.The technical problem to be solved in the present invention first is that cyanobacteria enrichment water body acute toxicity evaluation, second is that the screening of health, the big Daphnia magna of body and raising and train.The feasibility handled using acute toxicity assessment application Daphnia magna, then by laboratory and scene it is two stage cultivation raise and train can in Blue-green Algae Fast Growth water body growth and breeding, it is ingested cyanobacteria using Daphnia magna, promote oxygen in water concentration, promote submerged plant growth, it is re-introduced into the high degree biological such as fishes and shrimps, spiral shell and forms closed cycle using Daphnia magna as bait, and then restore original aquatic ecosystem, eliminate cyanobacteria pollution.
Description
Technical field
The present invention relates to water-treatment technology field, be using ecosystem restoration as the water body of principle and waters purification method,
A kind of more particularly to method of method and water purifying based on Daphnia magna removal water body cyanobacteria.
Background technique
Cyanobacteria also known as blue-green alge (blue-green algae), be long a kind of evolutionary history, Gram-negative,
Atrichia contains chlorophyll a, but without chloroplaset (being different from Eukaryotic algae), can be carried out that produce oxygen photosynthetic big
Type unicellular prokaryotic.Be with photosynthetic bacteria difference: photosynthetic bacteria (red spirillum) carries out more original photophosphorylation and makees
With it is anaerobic organism that reaction process, which does not put oxygen, and cyanobacteria can be carried out photosynthesis and put oxygen.Its development makes entirely
Ball atmosphere develops to aerobic state from anaerobic state, to breed the evolution and development of all aerobes.So far have
More than 120 kinds of cyanobacterias have nitrogen fixing capacity, the especially water with Azolla imbricata fish raw meat cyanobacteria (Anabaena azollae) symbiosis
Raw fern Azolla imbricata, is a kind of good green manure.But some cyanobacterias are causing eutrophy after by element pollutions such as nitrogen, phosphorus
The seawater " red tide " of change and " wawter bloom " in lake, bring serious harm to fishery and aquaculture.
Currently, social development, the size of population increase sharply, mankind's water consumption is caused to sharply increase.Largely without being effectively treated,
Sanitary sewage, industrial wastewater containing higher concentration nitrogen and/or phosphorus are discharged into natural water wantonly, more than the self-purification capacity of water body,
Cause water eutrophication.The most important performance of one kind of water eutrophication is grown by the alga eruption of representative of cyanobacteria,
One layer of blue-green is gathered into the water surface and has the shielding layer of bad smell, is hindered the mass exchange of air and water, is led to water body
Anoxic, the cyanobacteria after death can also largely consume water oxygen, further decrease dissolved oxygen concentration in water body, water body is caused to become
Black, fouling, economic fish etc. destroy original aquatic ecosystem because of anoxia asphyxia death.
It in the prior art, can be with Applied Physics method, chemical method and bioanalysis etc. in order to go cyanobacteria in water removal.
Physical method, mainly using the artificial or mechanical method salvaged.This method is general by manually with net fishing
Or circulating filtration etc. is carried out with pump sucking water body.This method time-consuming, high labor cost, processing area are small, and filter out
Cyanobacteria also needs to continue advanced treating, therefore less use in practice.In addition to artificial or mechanical salvaging, there are also ultrasonic waves
Method, film filtering, the methods of blowing aeration, but that there are input costs is excessively high, removal effect is low etc. that disadvantages lead to nothing for these methods
Method is promoted.
Chemical method, mainly Xiang Shuizhong add chemical agent and quickly kill cyanobacteria, generally as a kind of emergency processing side
Method.But this method can also cause the death of the organisms such as fish, or even can endanger not only without reducing the nutriments such as nitrogen, phosphorus
Evil drinking water source safety.And this method does not have sustainability, once chemical agent fails, cyanobacteria can also breed again.
With the development of science and technology, biotechnology is more and more more paid close attention to by people.Research application at present is more
Biotechnology to be the principle based on food chain prey on algae using aquatic animal, such as by algophagous filter-feeder fish
Class is put into contaminated water body to inhibit the undue growth of algae.But alga cells have mostly special phycocolloid layer and
Polysaccharose substance, these phycocolloid and polysaccharose substance can hardly be digested by filter-feeding fish, absorb, be utilized, and be excreted
Or algae.In addition, the water body of cyanobacteria enrichment can cause water hypoxia, and algae toxin can be also discharged after cyanobacteria death, it is above-mentioned
Reason causes directly to apply fish that cyanobacteria in water removal is gone to be difficult practical application.
CN104787893A discloses acclimation method and the application of a kind of Daphnia magna, comprising: launches Daphnia magna to domestication
Chi Zhong for a period of time using specific domestication foodstuff culture takes sociales to carry out repeating domestication, screening, then through stable and multiple
It is strong, Daphnia magna domestication is finally become to the natural enemy of cyanobacteria.However, this acclimation method is excessively complicated, specific foodstuff of taming is matched
Side is increasingly complex, at high cost, therefore, it is impossible to be widely used.
For these reasons, how to provide a kind of simple, convenient and can be suitable for widely used based on Daphnia magna
The method for removing water body cyanobacteria, becomes technical problem urgently to be resolved
Summary of the invention
The present invention is directed to for the natural waters such as lake, reservoir because receive the sanitary sewage containing a large amount of nitrogen and phosphorus elements,
Industrial wastewater etc. causes Blue-green Algae Fast Growth problem, based on Daphnia magna and cyanobacteria food chain relation, develops one kind with ecological system degradation
It goes to eliminate cyanobacteria in water for the biological method of principle.
The present invention provides a kind of simple, convenient and can be suitable for widely used blue based on Daphnia magna removal water body
The method of algae, includes the following steps:
The toxicity of step 1) evaluating water quality: taking target water body, with the Daphnia magna offspring of same parent of the same age, cultivate 1-3 generation,
The young Magna of birth 6-24h is used to evaluate the water quality toxicity of the target water body；Target water sample is diluted with the tap water of natural aeration
As experiment liquid, target water sample is 1:9 with originally water volume ratio, if 3 parallel laboratory tests, every part of experimental liquid product 200-
Daphnia magna is added in 1000ml, and the Daphnia magna additional amount is that every 1L tests addition children Magna 200-500 in liquid, cultivates 48h, note
Record remain to movable Daphnia magna number, when Daphnia magna movable inhibiting rate be lower than 50%, that is, judge target water body to the urgency of Daphnia magna
Property toxicity 48h EC50>=100mg/L saves backup the target water body for meeting acute toxicity condition；
The experiment of step 2) Daphnia magna is raised and train: target water body is mixed with scenedesmus obliquus culture solution according to the volume ratio of 1:4
The first mixed water body is obtained, Daphnia magna is added in the first mixed water body of Xiang Suoshu, the additional amount of the Daphnia magna is every 500ml
The mixed water body in be added 100-250 Daphnia magna, cultivated under culture environment for 24 hours, to Daphnia magna counting, when
Daphnia magna survival rate >=80% then continues subsequent incubation；
Wherein, the culture environment are as follows: natural lighting indoors, in the case where avoiding direct sunlight, cultivation temperature control
At 15-25 DEG C, the organic carbon concentration of the scenedesmus obliquus culture solution is controlled in 250-350mg/L；
Then the target water body second is mixed to get according to the volume ratio of 1:3 with the scenedesmus obliquus culture solution to mix
Heshui body continues the Daphnia magna Jing Guo the first mixed water body culture in second mixed water body, in the culture
Continue culture under environment for 24 hours；
It is successively that 1:2,1:1,2:1,3:1 and 4:1 match with the scenedesmus obliquus nutrient solution volume ratio by the target water body
Make different mixed water bodies, make Daphnia magna by culture successively in the increased mixed water body of target water body content,
Continue culture under the culture environment for 24 hours, will finally put into target water body, by the Daphnia magna of culture in the culture
Continue culture 3 weeks, the Daphnia magna after being raised and train under environment；
Raise and train at the scene of step 3) Daphnia magna: at target water body scene, the waters for selecting cyanobacteria more is put into 1m × 1m's
Net cage puts into the Daphnia magna after described raise and train in net cage, and the concentration of the Daphnia magna after raising and train described in holding is 100-
300ind/L, carry out scene are raised and train 2-3 weeks；
Step 4) removes cyanobacteria: the Daphnia magna that step 3) has been raised and train uniformly launched in target water body, it is lasting to launch
Until the concentration of Daphnia magna is maintained at 100-300ind/L.
Further, the pH value of the culture environment is controlled 7.5 ± 0.5.
Further, the dissolved oxygen of the culture environment is 2-7mg/L.
Further, the formula of the scenedesmus obliquus culture solution are as follows: contain ammonium sulfate 0.2g in every 1L culture solution, cross phosphorus
Sour calcium saturated solution 1ml, magnesium sulfate 0.08g, sodium bicarbonate 0.1g, potassium chloride 0.025g.
Further, by the dispensing of the step 4), the water transparency of the target water body reaches 1.5-2m.
Further, when the water transparency reaches 1.5-2m, fish, shrimp, shellfish are introduced in Xiang Suoshu target water body
Continue to optimize water body environment.
Further, the target water body by fish, shrimp, shellfish continue optimization after, where the target water body
Emergent aquactic plant, floatingleaved plant and/or submerged plant are introduced in waters.
Further, the emergent aquactic plant includes lotus, reed, the stem or leaf of cattail, Chinese celery and/or Scirpus tabernaemontani.
Further, the floatingleaved plant includes water lily, spatterdock, floating heart, water chestnut Ye Shuilong.
Further, the submerged plant includes hornwort, black algae, water quality grass, eel grass, water caltrop.
The technical problem to be solved in the present invention first is that cyanobacteria enrichment water body acute toxicity evaluation, second is that health, body are big
It the screening of Daphnia magna and raises and train.The feasibility handled using acute toxicity assessment application Daphnia magna, then passes through laboratory
With the two stage cultivation in scene raise and train can in Blue-green Algae Fast Growth water body growth and breeding, ingested cyanobacteria, promoted using Daphnia magna
Oxygen in water concentration promotes submerged plant growth, is re-introduced into the high degree biological such as fishes and shrimps, spiral shell using Daphnia magna as bait
Closed cycle is formed, and then restores original aquatic ecosystem, eliminates cyanobacteria pollution.
The increase of Water, P elements promotes algae especially cyanobacteria madness to breed and then break out wawter bloom.When water body is sudden and violent
When shampoo China, water surface cyanobacteria will affect the mass exchange of water body and atmosphere, and the sunlight intensity being lowered into water is led
Water hypoxia is caused to cause the aquatic animals death by suffocation such as fish.The degradation of the aquatic animals such as dead cyanobacteria and fish can be further
The dissolved oxygen content in water is reduced, causes water body stench occur, destroys original aquatic ecosystem.
Daphnia magna is cladocera aquatic animal, using algae as food source, it can in Different Waters growth and breeding, and have
Stronger adaptability, for example, in test, being exposed to after the toxic Microcystis aeruginosa of low concentration, Daphnia magna can adapt to simultaneously
Develop into the tolerance to it.
Daphnia magna is introduced into the water body of cyanobacteria enrichment, can thoroughly digest cyanobacteria, and the metabolite of its release, can
To reduce the pH value of water body, inhibit with Microcystis aeruginosa to be the regeneration for representing cyanobacteria.Water transparency after water body cyanobacteria reduces, disappears
Increase, water body projects luminous intensity and increases, and promotes benthic growth, reduces Water, phosphorus concentration, further suppresses indigo plant
Algae propagation.After Restoration of Benthos, be conducive to the growth and breeding of aquatic animal, restore original ecosystem self-purification function, object
Mass-energy is measured to recycle.It is then gradually introduced into water body high aquatic dynamic by the fish of bait, shrimp, spiral shell, shellfish etc. of Daphnia magna
Object repairs original aquatic ecosystem, and reduces the nutriments such as Water phosphorus by fishings such as fish, shrimp, spiral shell, shellfishes, realizes
Thoroughly remove Blue-green Algae Fast Growth risk.
Using ecological system degradation as principle, the water body treating and water of cyanobacteria food chain relation can be preyed on based on Daphnia magna
Ecological restoring method, the steps include: selected target water body first, the water quality of evaluation goal water body to the acute toxicity of Daphnia magna,
When acute toxicity is in following (the i.e. 48h EC of low toxicity50>=100mg/L) when, in laboratory controlled state scenedesmus obliquus and mesh
Water body (water body of cyanobacteria enrichment) mixed liquor is marked to feed Daphnia magna, referring concurrently to the water standard of target water body, is changed large-scale
The living environment of Magna gradually reduces the concentration for feeding scenedesmus obliquus in liquid, improves target water body and is feeding the ratio in liquid, directly
To the nursing all target water bodys of liquid.After being fed 3 weeks with target water body, Daphnia magna adapts to the environmental condition of target water body substantially,
The process of raising and train is transplanted to polluted-water scene.At polluted-water scene, delimit certain area dispensing and raise and train rear Daphnia magna, see
Daphnia magna growing state and water transparency variation are examined, after raising and train 2-3 weeks, assesses regulation effect.Finally, it is continuous periodically with
The Daphnia magna tamed uniformly is launched target and administered in water body by low concentration, these Daphnia magnas are food with cyanobacteria, to blue in water
Concentration of algae is substantially reduced, water transparency is improved to 1.5-2m, can obviously observe benthon after be put into specific fish thereto
Kind, Daphnia magna is eaten up by fish again, is finally reached the purpose for administering cyanobacteria in water.
The beneficial effects of the present invention are:
1, process is raised and train for Daphnia magna, it is only necessary to target water body and scenedesmus obliquus culture solution are used, without adding again
Add additional material or foodstuff formula, greatly reduce the simple degree of operation, and greatly reduces cost；
2, it is of the invention raise and train during, several indexs of novelty discovery water body exist with the domestication of Daphnia magna to be associated with,
This is not reported in other prior arts, meanwhile, the water quality condition of reference object water body, after Daphnia magna culture domestication, in mesh
The transparency that can be eliminated cyanobacteria under mark water body, improve water body, inhibits breaking out once again for cyanobacteria, establishes free of contamination ecological ring
Border；
3, the waters handled by method of the invention, can restore the self-purification capacity in waters, form new generation circulation
System.
Specific embodiment
Hereinafter, certain exemplary embodiments are simply just described.As one skilled in the art will recognize that
Like that, without departing from the spirit or scope of the present invention, described implement can be modified by various different modes
Example.Therefore, embodiment is considered essentially illustrative rather than restrictive.
Embodiment one
The toxicity of step 1) evaluating water quality: the Daphnia magna offspring for the same parent of the same age for selecting healthy body big first, culture 1
Generation, birth 6h young Magna be used to evaluate the water quality toxicity of the target water body；Target water sample is diluted with the tap water of natural aeration
As experiment liquid, target water sample is 1:9 with originally water volume ratio, and Daphnia magna is added in every part of experimental liquid product 200ml, described big
Type Magna additional amount is that every 1L tests addition children Magna 500 in liquid, cultivates 48h, record remains to movable Daphnia magna number, works as large size
The movable inhibiting rate of Magna is lower than 50%, that is, judges target water body to the acute toxicity 48h C of Daphnia magna50>=100mg/L will be accorded with
The target water body for closing acute toxicity condition saves backup；
Step 2): the experiment of Daphnia magna is raised and train: taking the glass of 2.5L, target water body and scenedesmus obliquus culture solution are pressed
It is mixed to get the first mixed water body according to the volume ratio of 1:4, Daphnia magna, the Daphnia magna are added in the first mixed water body of Xiang Suoshu
Additional amount be every 500ml the mixed water body in be added 100 Daphnia magnas, cultivated under culture environment for 24 hours, it is right
Daphnia magna counts, and when Daphnia magna survival rate >=80%, then continues subsequent incubation；
Wherein, the culture environment are as follows: natural lighting indoors, in the case where avoiding direct sunlight, cultivation temperature control
At 15 DEG C, in 350mg/L, pH value control is dissolved 7.5 ± 0.5 for the organic carbon concentration control of the scenedesmus obliquus culture solution
Oxygen is 2mg/L；
The formula of the scenedesmus obliquus culture solution are as follows: contain ammonium sulfate 0.2g, calcium superphosphate saturated solution in every 1L culture solution
1ml, magnesium sulfate 0.08g, sodium bicarbonate 0.1g, potassium chloride 0.025g；
Then after the target water body and the scenedesmus obliquus culture solution are mixed to get second according to the volume ratio of 1:3
Second mixed water body is added in the glass of 2.5L by mixed water body, will be by the first mixed water body culture
Daphnia magna continues in second mixed water body, continues culture under the culture environment for 24 hours；In the culture ring
It is cultivated under border for 24 hours, Daphnia magna is counted, when Daphnia magna survival rate >=80%, then continues subsequent incubation；
Then after the target water body and the scenedesmus obliquus culture solution are mixed to get third according to the volume ratio of 1:2
The third mixed water body is added in the glass of 2.5L by mixed water body, will be by the second mixed water body culture
Daphnia magna continues in the third mixed water body, continues culture under the culture environment for 24 hours；In the culture ring
It is cultivated under border for 24 hours, Daphnia magna is counted, when Daphnia magna survival rate >=80%, then continues subsequent incubation；
Then after the target water body and the scenedesmus obliquus culture solution are mixed to get the 4th according to the volume ratio of 1:1
4th mixed water body is added in the glass of 2.5L by mixed water body, will be by the third mixed water body culture
Daphnia magna continues in the 4th mixed water body, continues culture under the culture environment for 24 hours；In the culture ring
It is cultivated under border for 24 hours, Daphnia magna is counted, when Daphnia magna survival rate >=80%, then continues subsequent incubation；
Then after the target water body and the scenedesmus obliquus culture solution are mixed to get the 5th according to the volume ratio of 2:1
5th mixed water body is added in the glass of 2.5L by mixed water body, will be by the 4th mixed water body culture
Daphnia magna continues in the 5th mixed water body, continues culture under the culture environment for 24 hours；In the culture ring
It is cultivated under border for 24 hours, Daphnia magna is counted, when Daphnia magna survival rate >=80%, then continues subsequent incubation；
Then after the target water body and the scenedesmus obliquus culture solution are mixed to get the 6th according to the volume ratio of 3:1
6th mixed water body is added in the glass of 2.5L by mixed water body, will be by the 5th mixed water body culture
Daphnia magna continues in the 6th mixed water body, continues culture under the culture environment for 24 hours；In the culture ring
It is cultivated under border for 24 hours, Daphnia magna is counted, when Daphnia magna survival rate >=80%, then continues subsequent incubation；
Then after the target water body and the scenedesmus obliquus culture solution are mixed to get the 7th according to the volume ratio of 4:1
7th mixed water body is added in the glass of 2.5L by mixed water body, will be by the 6th mixed water body culture
Daphnia magna continues in the 7th mixed water body, continues culture under the culture environment for 24 hours；In the culture ring
It is cultivated under border for 24 hours, Daphnia magna is counted, when Daphnia magna survival rate >=80%, then continues subsequent incubation；
It is final to be directly added in the glass of 2.5L using target water body, the 7th mixed water body culture will be passed through
Daphnia magna continuation cultivated 3 weeks in the target water body, the Daphnia magna after being raised and train；
Raise and train at the scene of step 3) Daphnia magna: at target water body scene, the waters for selecting cyanobacteria more is put into 1m × 1m's
Net cage puts into the Daphnia magna after described raise and train in net cage, and the concentration of the Daphnia magna after raising and train described in holding is 100ind/
L, carry out scene are raised and train 3 weeks；
4) it removes cyanobacteria: the Daphnia magna that step 3) has been raised and train uniformly is launched in target water body, it is lasting to launch until big
The concentration of type Magna is maintained at 300ind/L.
The water quality condition for continuing monitoring objective water body scene introduces fish, shrimp, shellfish when water transparency reaches 1.5-2m
Class, and water body plant is planted, so that target water body water proximate forms the ecological balance, restore the ring of target water body water proximate
Border self-reparing capability.
Embodiment two
The toxicity of step 1) evaluating water quality: the Daphnia magna offspring for the same parent of the same age for selecting healthy body big first, culture 3
In generation, the young Magna of birth for 24 hours, are used to evaluate the water quality toxicity of the target water body；Target water is diluted with the tap water of natural aeration
Sample is 1:9 with originally water volume ratio as experiment liquid, target water sample, and Daphnia magna, institute is added in every part of experimental liquid product 1000ml
Stating Daphnia magna additional amount is that every 1L tests addition children Magna 200 in liquid, cultivates 48h, and record remains to movable Daphnia magna number, when
The movable inhibiting rate of Daphnia magna is lower than 50%, that is, judges target water body to the acute toxicity 48h C of Daphnia magna50>=100mg/L,
The target water body for meeting acute toxicity condition is saved backup；
Step 2): the experiment of Daphnia magna is raised and train: taking the glass of 2.5L, target water body and scenedesmus obliquus culture solution are pressed
It is mixed to get the first mixed water body according to the volume ratio of 1:4, Daphnia magna, the Daphnia magna are added in the first mixed water body of Xiang Suoshu
Additional amount be every 500ml the mixed water body in be added 250 Daphnia magnas, cultivated under culture environment for 24 hours, it is right
Daphnia magna counts, and when Daphnia magna survival rate >=80%, then continues subsequent incubation；
Wherein, the culture environment are as follows: natural lighting indoors, in the case where avoiding direct sunlight, cultivation temperature control
At 25 DEG C, in 250mg/L, pH value control is dissolved 7.5 ± 0.5 for the organic carbon concentration control of the scenedesmus obliquus culture solution
Oxygen is 7mg/L；
The formula of the scenedesmus obliquus culture solution are as follows: contain ammonium sulfate 0.2g, calcium superphosphate saturated solution in every 1L culture solution
1ml, magnesium sulfate 0.08g, sodium bicarbonate 0.1g, potassium chloride 0.025g；
Then after the target water body and the scenedesmus obliquus culture solution are mixed to get second according to the volume ratio of 1:3
Second mixed water body is added in the glass of 2.5L by mixed water body, will be by the first mixed water body culture
Daphnia magna continues in second mixed water body, continues culture under the culture environment for 24 hours；In the culture ring
It is cultivated under border for 24 hours, Daphnia magna is counted, when Daphnia magna survival rate >=80%, then continues subsequent incubation；
Then after the target water body and the scenedesmus obliquus culture solution are mixed to get third according to the volume ratio of 1:2
The third mixed water body is added in the glass of 2.5L by mixed water body, will be by the second mixed water body culture
Daphnia magna continues in the third mixed water body, continues culture under the culture environment for 24 hours；In the culture ring
It is cultivated under border for 24 hours, Daphnia magna is counted, when Daphnia magna survival rate >=80%, then continues subsequent incubation；
Then after the target water body and the scenedesmus obliquus culture solution are mixed to get the 4th according to the volume ratio of 1:1
4th mixed water body is added in the glass of 2.5L by mixed water body, will be by the third mixed water body culture
Daphnia magna continues in the 4th mixed water body, continues culture under the culture environment for 24 hours；In the culture ring
It is cultivated under border for 24 hours, Daphnia magna is counted, when Daphnia magna survival rate >=80%, then continues subsequent incubation；
Then after the target water body and the scenedesmus obliquus culture solution are mixed to get the 5th according to the volume ratio of 2:1
5th mixed water body is added in the glass of 2.5L by mixed water body, will be by the 4th mixed water body culture
Daphnia magna continues in the 5th mixed water body, continues culture under the culture environment for 24 hours；In the culture ring
It is cultivated under border for 24 hours, Daphnia magna is counted, when Daphnia magna survival rate >=80%, then continues subsequent incubation；
Then after the target water body and the scenedesmus obliquus culture solution are mixed to get the 6th according to the volume ratio of 3:1
6th mixed water body is added in the glass of 2.5L by mixed water body, will be by the 5th mixed water body culture
Daphnia magna continues in the 6th mixed water body, continues culture under the culture environment for 24 hours；In the culture ring
It is cultivated under border for 24 hours, Daphnia magna is counted, when Daphnia magna survival rate >=80%, then continues subsequent incubation；
Then after the target water body and the scenedesmus obliquus culture solution are mixed to get the 7th according to the volume ratio of 4:1
7th mixed water body is added in the glass of 2.5L by mixed water body, will be by the 6th mixed water body culture
Daphnia magna continues in the 7th mixed water body, continues culture under the culture environment for 24 hours；In the culture ring
It is cultivated under border for 24 hours, Daphnia magna is counted, when Daphnia magna survival rate >=80%, then continues subsequent incubation；
It is final to be directly added in the glass of 2.5L using target water body, the 7th mixed water body culture will be passed through
Daphnia magna continuation cultivated 3 weeks in the target water body, the Daphnia magna after being raised and train；
Raise and train at the scene of step 3) Daphnia magna: at target water body scene, the waters for selecting cyanobacteria more is put into 1m × 1m's
Net cage puts into the Daphnia magna after described raise and train in net cage, and the concentration of the Daphnia magna after raising and train described in holding is 300ind/
L, carry out scene are raised and train 3 weeks；
4) it removes cyanobacteria: the Daphnia magna that step 3) has been raised and train uniformly is launched in target water body, it is lasting to launch until big
The concentration of type Magna is maintained at 100ind/L.
The water quality condition for continuing monitoring objective water body scene introduces fish, shrimp, shellfish when water transparency reaches 1.5-2m
Class, and water body plant is planted, so that target water body water proximate forms the ecological balance, restore the ring of target water body water proximate
Border self-reparing capability.
The above description is merely a specific embodiment, but scope of protection of the present invention is not limited thereto, appoints
What those familiar with the art in the technical scope disclosed by the present invention, can readily occur in its various change or replace
It changes, these should be covered by the protection scope of the present invention.Therefore, protection scope of the present invention should be with the claim
Protection scope subject to.
Claims (10)
1. a kind of method based on Daphnia magna removal water body cyanobacteria, which comprises the steps of:
The toxicity of step 1) evaluating water quality: taking target water body, with the Daphnia magna offspring of same parent of the same age, cultivates 1-3 generation, birth 6-
Young Magna for 24 hours is used to evaluate the water quality toxicity of the target water body；The tap water of natural aeration is used to dilute target water sample as in fact
Liquid is tested, target water sample is 1:9 with originally water volume ratio, if 3 parallel laboratory tests, every part of experimental liquid product 200-1000ml is added
Daphnia magna, the Daphnia magna additional amount are that every 1L tests addition children Magna 200-500 in liquid, cultivate 48h, record remains to movable
Daphnia magna number, when Daphnia magna movable inhibiting rate be lower than 50%, that is, judge target water body to the acute toxicity 48h EC of Daphnia magna50
>=100mg/L saves backup the target water body for meeting acute toxicity condition；
The experiment of step 2) Daphnia magna is raised and train: target water body is mixed to get with scenedesmus obliquus culture solution according to the volume ratio of 1:4
First mixed water body, Daphnia magna is added in the first mixed water body of Xiang Suoshu, and the additional amount of the Daphnia magna is the described of every 500ml
100-250 Daphnia magna is added in mixed water body, is cultivated for 24 hours, Daphnia magna is counted, when Daphnia magna is deposited under culture environment
Motility rate >=80% then continues subsequent incubation；
Wherein, the culture environment are as follows: natural lighting indoors, in the case where avoiding direct sunlight, cultivation temperature is controlled in 15-
25 DEG C, the organic carbon concentration of the scenedesmus obliquus culture solution is controlled in 250-350mg/L；
Then the target water body and the scenedesmus obliquus culture solution are mixed to get the second mixing water according to the volume ratio of 1:3
Body continues the Daphnia magna Jing Guo the first mixed water body culture in second mixed water body, in the culture environment
Under continue culture for 24 hours；
It is successively that 1:2,1:1,2:1,3:1 and 4:1 are prepared not by the target water body and the scenedesmus obliquus nutrient solution volume ratio
With mixed water body, make Daphnia magna by culture successively in the increased mixed water body of target water body content, described
Continue culture under culture environment for 24 hours, will finally be put into target water body, under the culture environment by the Daphnia magna of culture
Continue culture 3 weeks, the Daphnia magna after being raised and train；
Raise and train at the scene of step 3) Daphnia magna: at target water body scene, the waters for selecting cyanobacteria more is put into the net cage of 1m × 1m,
Daphnia magna after described raise and train to be put into net cage, the concentration of the Daphnia magna after raising and train described in holding is 100-300ind/L,
It raises and train 2-3 weeks at carry out scene；
Step 4) removes cyanobacteria: the Daphnia magna that step 3) has been raised and train uniformly is launched in target water body, lasting dispensing until
The concentration of Daphnia magna is maintained at 100-300ind/L.
2. the method according to claim 1, wherein the pH value of the culture environment is controlled 7.5 ± 0.5.
3. method according to claim 1 or 2, feature is in the dissolved oxygen of the culture environment is 2-7mg/L.
4. according to the method described in claim 3, it is characterized in that, the formula of the scenedesmus obliquus culture solution are as follows: every 1L culture
Contain ammonium sulfate 0.2g, calcium superphosphate saturated solution 1ml, magnesium sulfate 0.08g, sodium bicarbonate 0.1g, potassium chloride 0.025g in liquid.
5. according to the method described in claim 4, it is characterized in that, dispensing by the step 4), the target water body
Water transparency reaches 1.5-2m.
6. according to the method described in claim 5, it is characterized in that, when the water transparency reaches 1.5-2m, Xiang Suoshu
Fish, shrimp, shellfish is introduced in target water body to continue to optimize water body environment.
7. a kind of method for carrying out waters purification using method described in any one of claims 1-6, which is characterized in that the mesh
Mark water body by fish, shrimp, shellfish continue optimization after, emergent aquactic plant, floating leaf are introduced in the waters where the target water body
Plant and/or submerged plant.
8. the method according to the description of claim 7 is characterized in that the emergent aquactic plant includes lotus, reed, the stem or leaf of cattail, Chinese celery
And/or Scirpus tabernaemontani.
9. according to the method described in claim 8, it is characterized in that, the floatingleaved plant includes water lily, spatterdock, floating heart, water chestnut
The leaf fire hose.
10. according to the method described in claim 9, it is characterized in that, the submerged plant include hornwort, black algae, water quality grass,
Eel grass, water caltrop.
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| CN113311144A (en) * | 2021-05-20 | 2021-08-27 | 江南大学 | Method for evaluating toxicity of micro-plastic based on behavioral indexes of daphnia magna |
| CN113800710A (en) * | 2021-08-31 | 2021-12-17 | 长江生态环保集团有限公司 | Device and method for combined remediation of eutrophic water body by composite biological treatment system |
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