CN104743672A - Water body eutrophication treatment technology based on daphnia magna domestication method - Google Patents
Water body eutrophication treatment technology based on daphnia magna domestication method Download PDFInfo
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- CN104743672A CN104743672A CN201510031412.6A CN201510031412A CN104743672A CN 104743672 A CN104743672 A CN 104743672A CN 201510031412 A CN201510031412 A CN 201510031412A CN 104743672 A CN104743672 A CN 104743672A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/007—Contaminated open waterways, rivers, lakes or ponds
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/30—Wastewater or sewage treatment systems using renewable energies
- Y02W10/37—Wastewater or sewage treatment systems using renewable energies using solar energy
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- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
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- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
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Abstract
The invention relates to a method for biologically eliminating and controlling the eutrophication of an open water body on the basis of a daphnia magna food chain relationship according to the principle of in-situ ecosystem remediation, and particularly relates to a daphnia magna domestication method. The method comprises the following steps: selecting a target water body to be treated; monitoring the water quality and pollution sources of the water body; according to the water quality and the pollution sources, domesticating and cultivating daphnia magna by stages with scenedesmus obliquus and eutrophic water mixed liquids until the daphnia magna is completely adapted to the environmental condition of the target water body to be treated, wherein the scenedesmus obliquus and eutrophic water mixed liquids have different scenedesmus obliquus concentrations; then continuously, regularly and uniformly putting the daphnia magna into the target water body according to a low concentration; and finally introducing specific fish, such as aristichthysnobilis, which feeds on the daphnia magna. Through the method, the problem of eutrophication of the open water body can be solved effectively, an original ecosystem is recovered, and in-situ remediation of the water body is realized.
Description
Technical field
The present invention relates to a kind of with ecosystem in-situ immobilization for principle, eliminate based on large cyclop food chain relation biology and prevent and treat the technology of open body eutrophication, especially the domestication of large cyclop and cultural method.
Background technology
Because the size of population is increased sharply and some irrational social activity of the mankind, the source of pollution such as sanitary sewage, trade effluent in a large number containing nitrogen, phosphorus enter natural water area, wantonly well beyond the self-purification capacity of water body.And after pollution exceedes certain limit, body eutrophication will be caused, a large amount of unicellular algae, especially blue-green algae, start explosive growth, these algae often float on the water surface, make the dissolved oxygen of water surface saturated, but also blocked the irradiation of sunlight to water body, make submerged plant to carry out normal photosynthesis, and then have influence on the survival and reproduction of the hydrocoles such as fish, even anoxia asphyxia and dead.Aerobic microorganism can reduce the dissolved oxygen content in water further to the animals and plants degraded after death, water ecosystem suffers serious destruction, serious threat HUMAN HEALTH.
The mankind have attempted various possible way to administer body eutrophication, comprise chemical method, Physical, biological process etc.
Xiang Shuizhong adds chemical algicide agent can kill most algae at short notice, but this chemical process does not only cut down the absolute quantity of water Middle nutrition material, also likely has an impact to fish and other biological, and even harm humans is healthy.And chemical treatment can not eliminate the remaining essential problem of nutrition, a bite chemical agent is decomposed or dilutes, and algae also can breed again in large quantities, and this just needs to add chemical agent continuously, and then produces more serious consequence.
Adopt physical method that is artificial or machinery salvaging, required mesh is too small, and the algae water content of salvaging is too high, and does not cut off the basic substance of blue-green algae outburst, and this unicellular algae can break out again, can not tackle the problem at its root.At present, in regional area, adopt the physics hold-up interception method of floated fence to tackle blue algae bloom.
As everyone knows, when in water body, dissolved oxygen concentration is higher, phosphoric acid salt exists mainly with chemical combination state; When dissolved oxygen concentration is lower, phosphoric acid salt will dissolve in a large number, and therefore blowing aeration method can reduce phosphatic solubleness, suppresses algae reproduction.But blowing aeration method can not solve the too high root problem of the nutrient content of water body own, can not avoid the outburst once again of algae.
Also have the method such as ultrasonic wave, membrane filtration, but these methods be not cost too higher position be cannot promotion and implementation.
Along with deepening continuously of scientific research, people expect to utilize biotechnology to disinthibite blue algae bloom from the angle of ecology, current research is the growth adopting hydrocoles or waterplant to suppress algae more widely, such as, algophagous filter-feeding fish is put into contaminated water body thus the hypertrophy of suppression algae.But alga cells has special phycocolloid layer and polysaccharose substance mostly, these phycocolloid and polysaccharose substance almost can not digest by fish, absorb and utilize, even if algae can be eaten up by fish, can not complete digestion, excrete or algae.In addition, be difficult to existence eutrophication water Mesichthyes, mainly because a large amount of dissolved oxygen consume by algae, fish are dead because of anoxia asphyxia, and some algae also can discharge toxin, directly produces toxic action to fish.
Summary of the invention
The present invention is directed to the body eutrophication that the open water body such as lake, reservoir causes because of the source of pollution such as sanitary sewage, trade effluent received containing a large amount of nitrogen, phosphoric, based on large cyclop food chain relation, develop a kind of biology being principle with ecosystem in-situ immobilization and go elimination and prevent and treat the method for open body eutrophication.
Large cyclop is a kind of filter-feeding cladocera zooplankton through improvement, it can in various severe water surrounding growth and breeding, more much bigger than wild cladocera individuality, food algae effect is very remarkable, physical environment has good stability, be easy to manipulation, and also have certain adsorption and enrichment effect to water pollutant.The biological strain of the 62Dm large cyclop that the present invention uses is the pure strain from CDC environment and health related products safe institute's ecotoxicological laboratory picking.This large cyclop is through separation, purifying after gathering from pond, Beijing Zoo in 1962, after under microscope (stereoscopic), large cyclop is confirmed as in qualification, take scenedemine as bait, large, the healthy parent of selective body is several carries out single cultivation, then selected reproduction amount maximum one on behalf of female water flea, the biological strain of the pure strain that mono-clonal obtains, Secondary Culture more than 40 year under same test conditions.
The technical problem to be solved in the present invention selects the health of pure strain, large cyclop that body is large, the two stage cultivation domestication in room and scene makes it adapt to gradually at eutrophication water environment and growth and breeding wherein by experiment, utilize large cyclop ingest blue-green algae promote submerged plant regeneration, introducing fish, shrimp, spiral shell, shellfish hydrocoles eat up large cyclop, set up an eucyclic Food-Chain System, thoroughly by nitrogen, phosphorus substance transfer, realize the purification of polluted-water.
With ecosystem in-situ immobilization for principle, based on the body eutrophication Treatment process of large cyclop acclimation method, it realizes walking mule and is:
First, water body is administered in selected target, monitors its variation of water and ambient contamination source situation.Large cyclop is fed with scenedesmus obliquus algae liquid and eutrophication water mixed solution in controlled experiment room, the water quality standard of reference object water body changes the living environment of large cyclop simultaneously, progressively reduce the concentration of feeding scenedesmus obliquus in liquid, improve target simultaneously and administer water body proportion, until the nursing liquid of large cyclop is all eutrophication water.
After feeding for some time with eutrophication water algae liquid, feed the medium-and-large-sized water flea density of liquid and reach 200/liter, and keep stable, illustrate that large cyclop adapts to the envrionment conditions of target water body substantially, domestication process is transplanted to scene, continues with eutrophication water domestication large cyclop.Upgrowth situation and the water quality changing conditions of large cyclop is observed between domestication.
Finally, regularly continuously the large cyclop of having tamed evenly rendered in the eutrophication water that target administers with lower concentration, these large cyclops take blue-green algae as food, in water, algae bio amount obviously reduces, water transparency is when reaching more than 0.5m, put into the specific fingerlings such as silver carp flathead wherein, large cyclop is eaten up by fish again, finally reaches the object of administering eutrophication water.
Beneficial effect of the present invention is: in eutrophication water, introduce large cyclop ingest after digestion eutrophication water blue-green algae, can discharge acid ight soil and meta-bolites, reduce the pH value of water body, suppress the regeneration of the Micro Algaes such as Microcystis aeruginosa.In water body, blue-green algae reduces the rear water transparency that disappears increases, and direct sunlight enters the bottom, promotes sprouting and the growth of the water-bed submerged plant of water body, forms bosk under water; Submerged plant carries out photosynthesis, produces a large amount of oxygen, sponges nitrogen phosphorus substance too much in water body, forms ecology of water self-cleaning, and produces allelopathy and suppress blue algae growth further.After aquatic vegetation remediation, be conducive to the growth and breeding of hydrocoles, under syngenetic process, form ecological chain self-purification function, matter energy is circulated.Progressively in water body, introduce the high hydrocoles such as fish, shrimp, spiral shell, shellfish, large cyclop can be eaten up by these hydrocoles, by food chain, the phosphorus and nitrogen in water body is migrated out from water body, reduces body eutrophication risk.
The water quality condition of the eutrophication water that reference object is administered, large cyclop is cultivated the transparency of taming into and can eliminating eutrophication water blue-green algae, raising water body in targeted environment, suppress breaking out once again of blue-green algae, set up free of contamination ecotope, reduce investment simultaneously, increase the benefit, simplify enforcement link, be convenient to system management.
Embodiment
It is objective for implementation that the present invention selectes eutrophication water in pool, rural area, Beijing.
The 62Dm pure strain large cyclop that enforcement the present invention selects the healthy body of Institute for Environment Hygiene and Health Related Product Safety, China CDC large carries out domestication and cultivates in controlled experiment indoor.Be add 1000ml in the glass beaker of 2L to cultivate water at volume, add 100-200 large cyclop.Under Indoor Natural optical condition, culture temperature 15 ~ 25 DEG C, pH is 7.5 ± 0.5, and dissolved oxygen is 2mg/L, avoids direct sunlight, feeds with scenedesmus obliquus algae liquid.After 24 hours, be that 40 ~ 60 orders screen filtration per square inch retains large cyclop by mesh, change the nutrient solution of large cyclop, nutrient solution is mixed by 900mL scenedesmus obliquus algae liquid and 100mL eutrophic water, continues cultivation 24 hours under identical indoor conditions; Then feed with 800mL scenedesmus obliquus algae liquid and 200mL eutrophication water mixed liquid, same method cultured continuously 10 days, until nutrient solution is all eutrophication water.Same operating process tames three times, one month by a definite date.
After controlled experiment indoor eutrophication water algae liquid domestication large cyclop is stable, the large cyclop process of taming is transferred to eutrophied water treatment scene.Under envrionment conditions at the scene, scenedesmus obliquus algae liquid and eutrophication water mixed solution is used to tame large cyclop.Domestication operating process is with in laboratory.Domestication process schedules to last January.
When on-the-spot domestication large cyclop to algae and water biomass is stablized, the large cyclop of having tamed is put into lower concentration the net cage being located at objective for implementation termly continuously, after large cyclop breeding reaches finite concentration, a desirable wherein part is put into the net cage set up and is processed other polluted waterss.
In order to avoid algae blocking mesh, net cage adopts anti-algae material to make, mesh is pressed 40-60 order/square inch and is arranged, net cage can be dragged to another waters after adopting the portable blue-green algae being convenient to former put area to be eaten up by net cage, the blue-green algae in whole waters can be eaten up gradually with Grazing system like this, in order to the ecotope impelling waters to form self-cleaning, can select to plant eel grass, all kinds of emergent such as water lily, floatingleaved plant and submerged plant, form bosk under water, a large amount of oxygen can be produced in growing process, large cyclop breathes the carbonic acid gas produced becomes again the photosynthetic carbon source of submerged plant, this symbiotic relationship can facilitate ecotope be more and more tending towards self-cleaning and form benign cycle, progressively recover original aquatic ecosystem, it is the preferably selection of administering waters blue-green algae.
Claims (9)
1. the biology based on large cyclop food chain relation is eliminated and prevents and treats the method for body eutrophication, it is characterized in that, after the selected eutrophication water that will administer, the mixed solution of target water body and scenedesmus obliquus algae liquid is used to tame large cyclop stage by stage, the water quality condition of foundation target water body progressively changes the growing environment of large cyclop simultaneously, makes it to adapt to the environment that target administers water body completely.The large cyclop of having tamed is rendered in target water body, the hydrocoles such as in good time introducing fish, shrimp, spiral shell, shellfish ingest large cyclop, not only effectively can administer body eutrophication, but also the original ecosystem of water body can be replied, realize the in-situ immobilization of water body.
2. large cyclop according to claim 1, is characterized in that: large, the healthy large cyclop of selective body carries out enlarged culturing.
3. domestication large cyclop according to claim 1, is characterized in that: the domestication process of large cyclop is divided into Laboratory Acclimation and two stages of on-the-spot domestication.The factors such as the domestication of large cyclop and water quality, abundance of food, temperature, dissolved oxygen are closely related.
4. in the Laboratory Acclimation stage according to claim 3, it is characterized in that: in the glass beaker of 2500mL, add 500-1000ml cultivate water, add 100-200 large cyclop.Under Indoor Natural optical condition, culture temperature 15 ~ 25 DEG C, pH is 7.5 ± 0.5, and dissolved oxygen is 2mg/L, avoids direct sunlight, the mixed solution of target of regularly throwing something and feeding water body and scenedesmus obliquus algae liquid.
5. in the on-the-spot domestication stage according to claim 3, it is characterized in that: the eutrophication water that the nutrient solution that on-the-spot domestication large cyclop uses is administered for target.
6. the mixed solution of target water body according to claim 4 and scenedesmus obliquus algae liquid, it is characterized in that: mixed by the scenedesmus obliquus of laboratory culture and eutrophication water, the concentration of scenedesmus obliquus liquid in fresh algae liquid is progressively turned down between large cyclop domestication, improve the ratio of eutrophication water, until fresh algae liquid is finally entirely eutrophication water simultaneously.
7. scenedesmus obliquus according to claim 6, is characterized in that: in scenedesmus obliquus liquid, concentration of algae is with 6 × 10
6individual/ml ~ 8 × 10
6individual/ml is best, and culture temperature is 15 ~ 25 DEG C, intensity of illumination is 3000 ~ 4000lx, light application time is 6 ~ 10h, and continuous static gas wave refrigerator, avoids direct sunlight.Want frequent microscopy, check whether pollutions such as being subject to other assorted algae, ciliate and wheel animalcule; Algae liquid will often be transferred, aging to prevent, the situation that transit time grows depending on algae liquid.The enlarged culturing of scenedesmus obliquus does not add any nutritive salt, and the grid algae liquid of enlarged culturing can direct feeding large cyclop.
8. large cyclop of raising and train according to claim 1 is rendered in target water body, it is characterized in that: regular with 200-300/liter evenly to be rendered in target water body by the large cyclop of having tamed continuously.
9. introducing fishes and shrimps spiral shell class hydrocoles according to claim 1, is characterized in that: when large cyclop concentration reaches every premium on currency 500-800, introduces the extraordinary fish such as silver carp flathead when water transparency reaches 0.5m.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107667929A (en) * | 2017-09-29 | 2018-02-09 | 四川中科水务科技有限公司 | A kind of breeding method of algae removal worm |
| CN107751057A (en) * | 2017-10-17 | 2018-03-06 | 广东工业大学 | A kind of Daphnia magna food chain type single cylinder conservation cultural method |
| CN107897127A (en) * | 2017-11-21 | 2018-04-13 | 佛山科学技术学院 | A kind of cultural method of purebred Daphnia magna for water ecology reparation |
| PL422553A1 (en) * | 2017-08-16 | 2019-02-25 | Mikronatura Środowiska Spółka Z Ograniczoną Odpowiedzialnością | Test of optimalization of the probiotic dosage into water environment, based on the reaction of Daphnia sp. (Cladocera, Daphnidae) crustaceans |
| CN109650546A (en) * | 2018-12-28 | 2019-04-19 | 中国疾病预防控制中心环境与健康相关产品安全所 | A kind of method of method and water purifying based on Daphnia magna removal water body cyanobacteria |
| WO2021011049A1 (en) * | 2019-07-15 | 2021-01-21 | Hewlett-Packard Development Company, L.P. | Beam lattice data in additive manufacturing |
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| CN101575144A (en) * | 2008-05-07 | 2009-11-11 | 何文辉 | Method for repairing water ecology |
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- 2015-01-15 CN CN201510031412.6A patent/CN104743672A/en active Pending
Patent Citations (1)
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| CN101575144A (en) * | 2008-05-07 | 2009-11-11 | 何文辉 | Method for repairing water ecology |
Non-Patent Citations (1)
| Title |
|---|
| 高世荣等: ""62Dm(大型水蚤)食藻虫生物株助力湖泊生态修复"", 《中国环境科学学会学术年会论文集（2010）》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PL422553A1 (en) * | 2017-08-16 | 2019-02-25 | Mikronatura Środowiska Spółka Z Ograniczoną Odpowiedzialnością | Test of optimalization of the probiotic dosage into water environment, based on the reaction of Daphnia sp. (Cladocera, Daphnidae) crustaceans |
| CN107667929A (en) * | 2017-09-29 | 2018-02-09 | 四川中科水务科技有限公司 | A kind of breeding method of algae removal worm |
| CN107751057A (en) * | 2017-10-17 | 2018-03-06 | 广东工业大学 | A kind of Daphnia magna food chain type single cylinder conservation cultural method |
| CN107751057B (en) * | 2017-10-17 | 2021-02-09 | 广东工业大学 | Chain type single-cylinder seed-preserving culture method for daphnia magna food |
| CN107897127A (en) * | 2017-11-21 | 2018-04-13 | 佛山科学技术学院 | A kind of cultural method of purebred Daphnia magna for water ecology reparation |
| CN109650546A (en) * | 2018-12-28 | 2019-04-19 | 中国疾病预防控制中心环境与健康相关产品安全所 | A kind of method of method and water purifying based on Daphnia magna removal water body cyanobacteria |
| WO2021011049A1 (en) * | 2019-07-15 | 2021-01-21 | Hewlett-Packard Development Company, L.P. | Beam lattice data in additive manufacturing |
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