CN107667929A - A kind of breeding method of algae removal worm - Google Patents
A kind of breeding method of algae removal worm Download PDFInfo
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- CN107667929A CN107667929A CN201710906476.5A CN201710906476A CN107667929A CN 107667929 A CN107667929 A CN 107667929A CN 201710906476 A CN201710906476 A CN 201710906476A CN 107667929 A CN107667929 A CN 107667929A
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- 241000195493 Cryptophyta Species 0.000 title claims abstract description 138
- 238000009395 breeding Methods 0.000 title claims abstract description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 127
- 239000003643 water by type Substances 0.000 claims abstract description 19
- 230000009286 beneficial effect Effects 0.000 claims abstract description 12
- 230000008901 benefit Effects 0.000 claims abstract description 11
- 238000000926 separation method Methods 0.000 claims abstract description 10
- 238000005273 aeration Methods 0.000 claims abstract description 6
- 238000011017 operating method Methods 0.000 claims abstract description 4
- 238000000034 method Methods 0.000 claims description 16
- 239000002028 Biomass Substances 0.000 claims description 5
- 241000894007 species Species 0.000 claims description 5
- 238000005070 sampling Methods 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 15
- 230000004083 survival effect Effects 0.000 abstract description 10
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 10
- 239000001301 oxygen Substances 0.000 description 10
- 229910052760 oxygen Inorganic materials 0.000 description 10
- 241000251468 Actinopterygii Species 0.000 description 9
- 150000003839 salts Chemical class 0.000 description 7
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 5
- 244000144980 herd Species 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 4
- 230000000050 nutritive effect Effects 0.000 description 4
- 238000000746 purification Methods 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- 241000238571 Cladocera Species 0.000 description 3
- 241000192700 Cyanobacteria Species 0.000 description 3
- 239000007789 gas Substances 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 238000003911 water pollution Methods 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000012851 eutrophication Methods 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 208000005223 Alkalosis Diseases 0.000 description 1
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- 241000239250 Copepoda Species 0.000 description 1
- 241001494246 Daphnia magna Species 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 241000700141 Rotifera Species 0.000 description 1
- 239000005422 algal bloom Substances 0.000 description 1
- 230000002340 alkalosis Effects 0.000 description 1
- 230000000845 anti-microbial effect Effects 0.000 description 1
- 239000000460 chlorine Substances 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000002360 explosive Substances 0.000 description 1
- 230000005923 long-lasting effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004899 motility Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000009182 swimming Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 230000035899 viability Effects 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/20—Culture of aquatic animals of zooplankton, e.g. water fleas or Rotatoria
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Engineering & Computer Science (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Environmental & Geological Engineering (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Marine Sciences & Fisheries (AREA)
- Microbiology (AREA)
- Hydrology & Water Resources (AREA)
- Animal Husbandry (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Farming Of Fish And Shellfish (AREA)
- Cultivation Of Seaweed (AREA)
Abstract
The invention discloses a kind of breeding method of algae removal worm, including following operating procedure：1) culture mediums of BG 11 are prepared；2) aeration 3d running water 150L is respectively placed with two containers；3) waters will be administered and is used as target water body, and take upper, middle and lower each 10L of layer water body to be mixed using hydrophore, and be concentrated into 50ml, the macrozooplankton and phytoplankton sociales that will be beneficial to remove algae in 50ml water bodys be separated；4) zooplankter in step 3) is placed in container one, phytoplankton is placed in container two；5) culture mediums of BG 11 are added in container two and growth culture is carried out to the advantage algae kind of separation；6) algae in container two is dragged in container one daily, injected volume by 6% be incremented by, continuous 5-8d, by taming, eliminate after zooplankter dominant population be algae removal worm；7) algae removal worm is dispensed；Using target water body culture algae removal worm, water body is better conformed to, survival rate is improved, except algae effect is more preferable.
Description
Technical field
The present invention relates to ecological restoration technical field, and in particular to a kind of breeding method of algae removal worm.
Background technology
As the development of industrial or agricultural, the growth of population and people are for the demand of more high standard of living, the discharge capacity of waste water
It is continuously increased.A kind of important sign that body eutrophication phenomenon is on the rise is that the extensive of algal bloom breaks out, wherein, most
Common is exactly blue-green alga bloom.The outburst of blue-green algae can form one layer of blue-green in the water surface and have the offscum of fishy smell, cause water quality to be disliked
Change, exhaust water oxygen when serious and cause the death of fish.Water PH value can be caused drastically to be increased to 9.5 during blue-green algae peak
Cause alkalosis above, strengthen the toxicity of ammonia nitrogen, cause water body animal poisoning etc..Algae control technology mainly has thing in lake at present
Logos, chemical method and Antimicrobial algae, animal predation method, bio operon method etc..But the method for ecology is trend of the times, more
It is safe, more stable, more longlasting, it is more attractive.
Existing method mainly changes its enteric microorganism composition using taming Magna, reach can digest the ability of blue-green algae so as to
Purify water；Or Daphnia magna is cultivated, and goals of investment water body after being tamed with blue-green alge powder.Phytoplankton is divided into
Cladocera, Copepods, wheel animalcule and protozoan.Currently used is the cladocera of single variety, is because cladocera is to swimming
Plant, that is, food rate is higher is herded to algae, but the zooplankter in different waters composition is different, and zooplankter
Adaptability to different water bodys is different, and administering water environment using the waters of same species at different conditions may
Cause the problems such as inadaptability, the survival rate of zooplankter are low, regulation effect is poor.
Based on this, there is provided a kind of breeding method of algae removal worm.
The content of the invention
It is an object of the invention to：For the method for existing cultivation algae removal worm, using fixed kind algae removal worm to all water
The wawter bloom in domain is administered, and the algae removal worm turned out is relatively low to water body adaptability and viability, is unable to reach expected net
Change effect, it is proposed that a kind of breeding method of algae removal worm, according to the theory of " original seed controls raw water ", separated using the water of target water body
Go out algae removal worm colony and algae kind, then by inoculating and culturing out the algae removal worm come, can be more easy to adapt to water body, survival rate is high, algae removal speed
Degree faster, solves algae removal worm in the prior art and does not adapt to water body, and survival rate is low, except technical problems such as algae effect differences.
The present invention is achieved through the following technical solutions：
A kind of breeding method of algae removal worm, including following operating procedure：
1) BG-11 culture mediums are configured；
2) aeration 3d running water 150L is respectively placed with two containers；
3) waters will be administered as target water body, take upper, middle and lower each 10L of layer water body to be mixed, and be concentrated into
50ml, it will be beneficial in 50ml water bodys except the macrozooplankton of algae and phytoplankton sociales are separated；
4) zooplankter in step 3) is placed in container one, phytoplankton is placed in container two；
5) BG-11 culture mediums are added in container two and growth culture is carried out to the advantage algae kind of separation；
6) algae in container two is dragged in container one daily, injected volume is incremented by by 6%, continuous 5-8d, by taming, washing in a pan
Zooplankter dominant population after eliminating is algae removal worm；
7) algae removal worm is dispensed, repeats the above steps and carry out growth culture；
Prior art cultivate algae removal worm method be：Next algae removal worm varities practice is turned out into all water in fixed waters
In the purification of water quality processing in domain, because algae kind is different in each waters, algae removal worm can not preferably adapt to water body, and survival rate is low,
The technical program is used to cultivate algae removal worm using the water body in improvement waters as target water body, and the algae removal worm cultivated is not of the same race
The zooplankter of class, algae removal speed is faster.
Meanwhile algae and algae removal worm are selected from the target water body administered in the technical program, can preferably adapt to water body, deposit
Motility rate is higher.
Running water after being put into Air Exposure in container one and container two in the technical program, the purpose master of Air Exposure
If removing chlorine-containing compound in running water, the influence to zooplankter, phytoplankton growth is avoided.
Preferably, the sampling method of target water body amount is in the step 3)：Taken respectively on target water body using hydrophore
Layer waters, midwater, each 10L in subjacent waters, mix and produce.
Preferably, the method that target water body amount 30L is concentrated into 50mL is to be concentrated using plankton net.
Preferably, the macrozooplankton and phytoplankton sociales that control algae is beneficial in the water body by 50ml are divided
From the operation progress under microscope and anatomical lens.
Preferably, the separation of the phytoplankton is carried out using capillary, the selection species that biomass is big and density is big, i.e.,
Needed in target water body control the problem of algae.
Preferably, BG-11 culture mediums are added in the step 5) in container two and growth training is carried out to the advantage algae kind of separation
Support, as the algae kind tamed to algae removal worm.
Preferably, algae in container two is dragged in container one daily in the step 5), injected volume is incremented by by 6%, continuously
5-8d, zooplankter is tamed, have certain tolerance to problem algae kind, the species finally left is algae removal worm.
Preferably, the step 7) dispenses algae removal worm, repeat the above steps carry out grow culture can obtain largely
Algae removal worm, then goals of investment water body administered.
The present invention compared with prior art, has the following advantages and advantages：
(1) the algae removal worm that the technical program is turned out utilizes lower row effects, and algae can be carried out to herd food, reduces algae life
Object amount, and algae removal worm is the live bait that fish are liked, is ingested by fish, is finally moved the nutritive salt in water in the form of fishery -ies product
Water-outlet body, the nutrient concentration in water is reduced, is enhanced the transparency, to build the stable state of clear water aquatic system based on submerged plant
Basic condition is provided.
(2) algae removal worm group is separated out using the moisture of target water body according to the theory of " original seed controls raw water " in the technical program
Body and algae kind, then by inoculating and culturing out the algae removal worm come, it can be more easy to adapt to water body, survival rate is high, and algae removal speed faster, solves
Algae removal worm does not adapt to water body in the prior art, and survival rate is low, except technical problems such as algae effect differences.
Embodiment
For the object, technical solutions and advantages of the present invention are more clearly understood, with reference to embodiment, the present invention is made
Further to describe in detail, exemplary embodiment of the invention and its explanation are only used for explaining the present invention, are not intended as to this
The restriction of invention.
Embodiment 1：
It is rain for urban landscape man-made lake, 15000 square metres of water surface area, mean depth 1.5m, main make-up water source
Water.Water body is muddy, and transparency is relatively low, and water colour is minimum in green, water body flow.
A kind of breeding method of algae removal worm, including following operating method：
1) BG-11 culture mediums are prepared；
2) two container contents devices one, container two are respectively placed with aeration 3d running water 150L；
3) waters will be administered as target water body, take upper, middle and lower each 10L of layer water body to be mixed using hydrophore, and
50ml is concentrated into, will be beneficial in 50ml water bodys except the macrozooplankton of algae and phytoplankton sociales are separated；
4) zooplankter in step 3) is placed in container one, phytoplankton is placed in container two；
5) BG-11 culture mediums are added in container two and growth culture is carried out to the advantage algae kind of separation；
6) algae in container two is dragged in container one daily, injected volume is incremented by by 6%, continuous 5-8d, by taming, washing in a pan
Zooplankter dominant population after eliminating is algae removal worm；
7) algae removal worm is dispensed, repeats the above steps and carry out growth culture.
Using algae removal worm administer water pollution specific method be：The algae removal worm cultivated through step 7) is according to 1 × 105~2
×105/m3Target water body is launched, oxygen increasing equipment is placed in target water body, it is ensured that dissolved oxygen concentration is more than in target water body
Equal to 2mg/L.
The effects of purification quality of algae removal worm is monitored, algae removal worm is carried out according to the standing crop biomass of algae removal worm and algae
Second discharge, continue to monitor, second of dispensing can be carried out as needed.
Algae and water density declines, and transparency rise, pH value declines, and ammonia nitrogen declines, and reaches beneficial to aquatic macrophyte
Survival requires, in stable state of clear water aquatic ecosystem of the water body component based on submerged plant, is finally reached the net scape U.S. of the clear gas of water
" submerged forest " landscape effect.
Algae in the present embodiment in target water body absorbs the eutrophy salt amount reproduction in water body, and excessive consumption water body is molten
Oxygen is solved, algae removal worm utilizes lower row effects, and algae is carried out to herd food, reduces algae bio amount, and algae removal worm is the work that fish are liked
Bait, ingested by fish, the nutritive salt in water finally removed into water body in the form of fishery -ies product, reduce nutrient concentration in water,
Enhance the transparency, basic condition is provided to build the stable state of clear water aquatic ecosystem based on submerged plant.
Embodiment 2：
For waters city river, total length 800m, 3~15m of river width, ordinary water level 1.25m, the river course is black and odorous water, water
Eutrophication is totally presented in body, and color greening, the explosive algae of turning blue of high temperature season, water quality is bad V class, in city river waters
The method for cultivating algae removal worm is as follows：
1) BG-11 culture mediums are prepared；
2) two containers, aeration 3d running water 150L is respectively placed with container one, container two；
3) waters will be administered as target water body, take upper, middle and lower each 10L of layer water body to be mixed using hydrophore, and
50ml is concentrated into, will be beneficial in 50ml water bodys except the macrozooplankton of algae and phytoplankton sociales are separated；
4) zooplankter in step 3) is placed in container one, phytoplankton is placed in container two；
5) BG-11 culture mediums are added in container two and growth culture is carried out to the advantage algae kind of separation；
6) algae in container two is dragged in container one daily, injected volume is incremented by by 6%, continuous 5-8d, by taming, washing in a pan
Zooplankter dominant population after eliminating is algae removal worm；
7) algae removal worm is dispensed, repeated the above steps 1) -6) carry out growth culture.
Using algae removal worm administer water pollution specific method be：The algae removal worm cultivated through step 7) is according to 1 × 105~2
×105/m3Target water body is launched, oxygen increasing equipment is placed in target water body, it is ensured that dissolved oxygen concentration is more than in target water body
Equal to 2mg/L.
The effects of purification quality of algae removal worm is monitored, algae removal worm is carried out according to the standing crop biomass of algae removal worm and algae
Second discharge, continue to monitor, second of dispensing can be carried out as needed.
Algae and water density declines, and transparency rise, pH value declines, and ammonia nitrogen declines, and reaches beneficial to aquatic macrophyte
Survival requires, in stable state of clear water aquatic ecosystem of the water body component based on submerged plant, is finally reached the net scape U.S. of the clear gas of water
" submerged forest " landscape effect.
Algae in the present embodiment in target water body absorbs the eutrophy salt amount reproduction in water body, and excessive consumption water body is molten
Oxygen is solved, algae removal worm utilizes lower row effects, and algae is carried out to herd food, reduces algae bio amount, and algae removal worm is the work that fish are liked
Bait, ingested by fish, the nutritive salt in water finally removed into water body in the form of fishery -ies product, reduce nutrient concentration in water,
Enhance the transparency, basic condition is provided to build the stable state of clear water aquatic ecosystem based on submerged plant.
Embodiment 3：
For middle advanced water treatment waters, area 5000m2, the index such as mean depth 1.5m, TN, TP, ammonia nitrogen exists larger
The exceeded phenomenon in Chengdu, water body are presented the higher feature of nitrogen and phosphorus pollutantses content, lack nourishment source, use reclaimed water conduct for a long time
Nourishment source, water quality is bad V class before reparation, transparency only 0.3m.The method that algae removal worm is cultivated in middle advanced water treatment waters
It is as follows：
1) BG-11 culture mediums are prepared；
2) two containers, aeration 3d running water 150L is respectively placed with container one, container two；
3) waters will be administered as target water body, take upper, middle and lower each 10L of layer water body to be mixed using hydrophore, and
50ml is concentrated into, will be beneficial in 50ml water bodys except the macrozooplankton of algae and phytoplankton sociales are separated；
4) zooplankter in step 3) is placed in container one, phytoplankton is placed in container two；
5) BG-11 culture mediums are added in container two and growth culture is carried out to the advantage algae kind of separation；
6) algae in container two is dragged in container one daily, injected volume is incremented by by 6%, continuous 5-8d, by taming, washing in a pan
Zooplankter dominant population after eliminating is algae removal worm；
7) algae removal worm is dispensed, repeated the above steps 1) -5) carry out growth culture.
Using algae removal worm administer water pollution specific method be：The algae removal worm cultivated through step 7) is according to 1 × 105~2
×105/m3Target water body is launched, oxygen increasing equipment is placed in target water body, it is ensured that dissolved oxygen concentration is more than in target water body
Equal to 2mg/L.
The effects of purification quality of algae removal worm is monitored, algae removal worm is carried out according to the standing crop biomass of algae removal worm and algae
Second discharge, continue to monitor, second of dispensing can be carried out as needed.
Algae and water density declines, and transparency rise, pH value declines, and ammonia nitrogen declines, and reaches beneficial to aquatic macrophyte
Survival requires, in stable state of clear water aquatic ecosystem of the water body component based on submerged plant, is finally reached the net scape U.S. of the clear gas of water
" submerged forest " landscape effect.
Algae in the present embodiment in target water body absorbs the eutrophy salt amount reproduction in water body, and excessive consumption water body is molten
Oxygen is solved, algae removal worm utilizes lower row effects, and algae is carried out to herd food, reduces algae bio amount, and algae removal worm is the work that fish are liked
Bait, ingested by fish, the nutritive salt in water finally removed into water body in the form of fishery -ies product, reduce nutrient concentration in water,
Enhance the transparency, basic condition is provided to build the stable state of clear water aquatic ecosystem based on submerged plant.
Above-described embodiment, the purpose of the present invention, technical scheme and beneficial effect are carried out further
Describe in detail, should be understood that the embodiment that the foregoing is only the present invention, be not intended to limit the present invention
Protection domain, within the spirit and principles of the invention, any modification, equivalent substitution and improvements done etc., all should include
Within protection scope of the present invention.
Claims (8)
- A kind of 1. algae removal worm breeding method, it is characterised in that：Including following operating procedure：1) BG-11 culture mediums are configured；2) two container contents devices one, container two are respectively placed with aeration 3d running water 150L；3) waters will be administered and is used as target water body, and take upper, middle and lower each 10L of layer water body to be mixed, and be concentrated into 50ml, general It is beneficial in 50ml water bodys except the macrozooplankton of algae and phytoplankton sociales are separated；4) zooplankter in step 3) is placed in container one, phytoplankton is placed in container two；5) BG-11 culture mediums are added in container two and growth culture is carried out to the advantage algae kind of separation；6) algae in container two is dragged in container one daily, injected volume by 6% be incremented by, continuous 5-8d, by taming, eliminate after Zooplankter dominant population be algae removal worm；7) algae removal worm is dispensed, repeated the above steps 1) -5) carry out growth culture.
- A kind of 2. breeding method of algae removal worm according to claim 1, it is characterised in that：Target water body in the step 3) The sampling method of amount is：It is utilized respectively hydrophore and takes target water body superjacent waters, midwater, each 10L in subjacent waters, mixing is .
- A kind of 3. breeding method of algae removal worm according to claim 1, it is characterised in that：It is described by target water body amount 30L The method for being concentrated into 50mL is to be concentrated using plankton net.
- A kind of 4. breeding method of algae removal worm according to claim 1, it is characterised in that：It is beneficial in the water body by 50ml The macrozooplankton and phytoplankton sociales for controlling algae are separated, and are operated and are carried out under microscope and anatomical lens.
- A kind of 5. breeding method of algae removal worm according to claim 1, it is characterised in that：The separation profit of the phytoplankton Carried out with capillary, selection biomass is big and density is big species, i.e., the problem of control is needed in target water body algae.
- A kind of 6. breeding method of algae removal worm according to claim 1, it is characterised in that：In the step 5) in container two Add BG-11 culture mediums and growth culture is carried out to the advantage algae kind of separation, as the algae kind tamed to algae removal worm.
- A kind of 7. breeding method of algae removal worm according to claim 1, it is characterised in that：It will hold daily in the step 5) Algae is dragged in container one in device two, and injected volume is incremented by by 6%, continuous 5-8d, zooplankter is tamed, to problem algae kind There is certain tolerance, the species finally left is algae removal worm.
- A kind of 8. breeding method of algae removal worm according to claim 1, it is characterised in that：The step 7) divides algae removal worm Dress, repeat the above steps 1) -5) carry out grow culture can obtain substantial amounts of algae removal worm, then goals of investment water body is controlled Reason.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113197133A (en) * | 2021-05-13 | 2021-08-03 | 珠江水利委员会珠江水利科学研究院 | A integration culture apparatus for zooplankton hatching breeds |
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Address after: No.19, section 4, Renmin South Road, Wuhou District, Chengdu, Sichuan 610000 Patentee after: Sichuan Zhongke High Tech Group Co.,Ltd. Address before: No.19, section 4, Renmin South Road, Wuhou District, Chengdu, Sichuan 610000 Patentee before: Sichuan Zhongke Water Group Co.,Ltd. Address after: No.19, section 4, Renmin South Road, Wuhou District, Chengdu, Sichuan 610000 Patentee after: Sichuan Zhongke Water Group Co.,Ltd. Address before: No.19, section 4, Renmin South Road, Wuhou District, Chengdu, Sichuan 610000 Patentee before: SICHUAN ZHONGKE WATER TECHNOLOGY CO.,LTD. |