CN108668965A - A kind of reduction bivalve shellfish Endophytic bacteria quantity, the cultural method of content of beary metal - Google Patents
A kind of reduction bivalve shellfish Endophytic bacteria quantity, the cultural method of content of beary metal Download PDFInfo
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- CN108668965A CN108668965A CN201810240198.9A CN201810240198A CN108668965A CN 108668965 A CN108668965 A CN 108668965A CN 201810240198 A CN201810240198 A CN 201810240198A CN 108668965 A CN108668965 A CN 108668965A
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- seawater
- chlorine
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- bivalve shellfish
- dechlorination
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- 235000015170 shellfish Nutrition 0.000 title claims abstract description 52
- 238000000034 method Methods 0.000 title claims abstract description 39
- 241000894006 Bacteria Species 0.000 title claims abstract description 27
- 229910052751 metal Inorganic materials 0.000 title claims abstract description 17
- 239000002184 metal Substances 0.000 title claims abstract description 17
- 230000009467 reduction Effects 0.000 title claims abstract description 12
- 239000013535 sea water Substances 0.000 claims abstract description 115
- 241000237502 Ostreidae Species 0.000 claims abstract description 53
- 235000020636 oyster Nutrition 0.000 claims abstract description 53
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 claims abstract description 52
- 239000000460 chlorine Substances 0.000 claims abstract description 52
- 229910052801 chlorine Inorganic materials 0.000 claims abstract description 52
- 238000006298 dechlorination reaction Methods 0.000 claims abstract description 40
- 239000000645 desinfectant Substances 0.000 claims abstract description 33
- AKHNMLFCWUSKQB-UHFFFAOYSA-L sodium thiosulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=S AKHNMLFCWUSKQB-UHFFFAOYSA-L 0.000 claims abstract description 18
- 235000019345 sodium thiosulphate Nutrition 0.000 claims abstract description 18
- 238000005273 aeration Methods 0.000 claims abstract description 11
- 239000011259 mixed solution Substances 0.000 claims abstract description 6
- 241000195493 Cryptophyta Species 0.000 claims description 44
- 229910001385 heavy metal Inorganic materials 0.000 claims description 25
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 24
- 235000016425 Arthrospira platensis Nutrition 0.000 claims description 23
- 240000002900 Arthrospira platensis Species 0.000 claims description 23
- 229940082787 spirulina Drugs 0.000 claims description 23
- 229920001661 Chitosan Polymers 0.000 claims description 16
- 239000002775 capsule Substances 0.000 claims description 15
- 238000004659 sterilization and disinfection Methods 0.000 claims description 13
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 12
- 241000195649 Chlorella <Chlorellales> Species 0.000 claims description 11
- 125000002057 carboxymethyl group Chemical group [H]OC(=O)C([H])([H])[*] 0.000 claims description 8
- 238000012258 culturing Methods 0.000 claims description 8
- 239000007788 liquid Substances 0.000 claims description 8
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims description 7
- ACTRVOBWPAIOHC-UHFFFAOYSA-N succimer Chemical compound OC(=O)C(S)C(S)C(O)=O ACTRVOBWPAIOHC-UHFFFAOYSA-N 0.000 claims description 7
- 239000011715 vitamin B12 Substances 0.000 claims description 7
- ZKQDCIXGCQPQNV-UHFFFAOYSA-N Calcium hypochlorite Chemical compound [Ca+2].Cl[O-].Cl[O-] ZKQDCIXGCQPQNV-UHFFFAOYSA-N 0.000 claims description 6
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims description 6
- 229930003268 Vitamin C Natural products 0.000 claims description 6
- 239000007844 bleaching agent Substances 0.000 claims description 6
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 6
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims description 6
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Inorganic materials [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 claims description 6
- 235000019154 vitamin C Nutrition 0.000 claims description 6
- 239000011718 vitamin C Substances 0.000 claims description 6
- 238000005286 illumination Methods 0.000 claims description 4
- 239000002904 solvent Substances 0.000 claims description 4
- 241000237536 Mytilus edulis Species 0.000 claims description 3
- 241000237509 Patinopecten sp. Species 0.000 claims description 3
- 239000003795 chemical substances by application Substances 0.000 claims description 3
- 235000020639 clam Nutrition 0.000 claims description 3
- 235000020638 mussel Nutrition 0.000 claims description 3
- 235000020637 scallop Nutrition 0.000 claims description 3
- 241000237519 Bivalvia Species 0.000 claims description 2
- 229930003451 Vitamin B1 Natural products 0.000 claims 1
- 229960003495 thiamine Drugs 0.000 claims 1
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 claims 1
- 239000011691 vitamin B1 Substances 0.000 claims 1
- 235000010374 vitamin B1 Nutrition 0.000 claims 1
- 238000000746 purification Methods 0.000 abstract description 11
- 210000003097 mucus Anatomy 0.000 abstract description 10
- 235000013372 meat Nutrition 0.000 abstract description 8
- 230000001580 bacterial effect Effects 0.000 abstract description 5
- 235000013305 food Nutrition 0.000 abstract description 5
- 239000000203 mixture Substances 0.000 abstract description 2
- 229910052802 copper Inorganic materials 0.000 description 11
- 239000010949 copper Substances 0.000 description 11
- 229910052793 cadmium Inorganic materials 0.000 description 10
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 8
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 description 7
- 239000004576 sand Substances 0.000 description 7
- 210000004027 cell Anatomy 0.000 description 5
- 239000003053 toxin Substances 0.000 description 5
- 231100000765 toxin Toxicity 0.000 description 5
- 238000009395 breeding Methods 0.000 description 4
- 230000001488 breeding effect Effects 0.000 description 4
- 239000004568 cement Substances 0.000 description 4
- 125000001309 chloro group Chemical group Cl* 0.000 description 4
- 238000001914 filtration Methods 0.000 description 4
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- 238000004140 cleaning Methods 0.000 description 3
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 description 3
- 230000002265 prevention Effects 0.000 description 3
- 239000006041 probiotic Substances 0.000 description 3
- 235000018291 probiotics Nutrition 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
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- 235000019159 vitamin B9 Nutrition 0.000 description 3
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- 241001465754 Metazoa Species 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000012851 eutrophication Methods 0.000 description 2
- JARKCYVAAOWBJS-UHFFFAOYSA-N hexanal Chemical compound CCCCCC=O JARKCYVAAOWBJS-UHFFFAOYSA-N 0.000 description 2
- 239000002054 inoculum Substances 0.000 description 2
- XMGQYMWWDOXHJM-UHFFFAOYSA-N limonene Chemical compound CC(=C)C1CCC(C)=CC1 XMGQYMWWDOXHJM-UHFFFAOYSA-N 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000003921 oil Substances 0.000 description 2
- KDYFGRWQOYBRFD-UHFFFAOYSA-N succinic acid Chemical compound OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 description 2
- GETQZCLCWQTVFV-UHFFFAOYSA-N trimethylamine Chemical compound CN(C)C GETQZCLCWQTVFV-UHFFFAOYSA-N 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 241001207609 Crassostrea hongkongensis Species 0.000 description 1
- 241000199914 Dinophyceae Species 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 241001466487 Phaeocystis Species 0.000 description 1
- 229930003779 Vitamin B12 Natural products 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 150000001299 aldehydes Chemical class 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- FDJOLVPMNUYSCM-WZHZPDAFSA-L cobalt(3+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+3].N#[C-].N([C@@H]([C@]1(C)[N-]\C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C(\C)/C1=N/C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C\C1=N\C([C@H](C1(C)C)CCC(N)=O)=C/1C)[C@@H]2CC(N)=O)=C\1[C@]2(C)CCC(=O)NC[C@@H](C)OP([O-])(=O)O[C@H]1[C@@H](O)[C@@H](N2C3=CC(C)=C(C)C=C3N=C2)O[C@@H]1CO FDJOLVPMNUYSCM-WZHZPDAFSA-L 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
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- 230000000249 desinfective effect Effects 0.000 description 1
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- 239000007789 gas Substances 0.000 description 1
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- 230000036449 good health Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 150000002576 ketones Chemical class 0.000 description 1
- 229940087305 limonene Drugs 0.000 description 1
- 235000001510 limonene Nutrition 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000009364 mariculture Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000003248 secreting effect Effects 0.000 description 1
- 239000001384 succinic acid Substances 0.000 description 1
- 238000002255 vaccination Methods 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 235000019163 vitamin B12 Nutrition 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/54—Culture of aquatic animals of shellfish of bivalves, e.g. oysters or mussels
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/72—Treatment of water, waste water, or sewage by oxidation
- C02F1/76—Treatment of water, waste water, or sewage by oxidation with halogens or compounds of halogens
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/08—Seawater, e.g. for desalination
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2303/00—Specific treatment goals
- C02F2303/04—Disinfection
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Marine Sciences & Fisheries (AREA)
- Food Science & Technology (AREA)
- Environmental Sciences (AREA)
- Insects & Arthropods (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Environmental & Geological Engineering (AREA)
- Birds (AREA)
- Hydrology & Water Resources (AREA)
- Biodiversity & Conservation Biology (AREA)
- Water Supply & Treatment (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Molecular Biology (AREA)
- Mycology (AREA)
- Physiology (AREA)
- Feed For Specific Animals (AREA)
- Fodder In General (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention provides a kind of reduction bivalve shellfish Endophytic bacteria quantity, the cultural methods of content of beary metal, belong to the technical field of bivalve shellfish purification, include the following steps：Chlorine-containing disinfectant with seawater mix and sterilizes 10~14h, be sterilized seawater, and the available chlorine content of the chlorine-containing disinfectant is 40~60wt%, and the quality of the chlorine-containing disinfectant and the volume ratio of seawater are (600~1350) g:(20~45) m3；Obtained disinfected sea water is mixed with sodium thiosulfate, after obtained 10~14h of mixed solution continuous aeration, obtains dechlorination seawater, the mass ratio of the sodium thiosulfate and chlorine-containing disinfectant is 1:(1~3)；By bivalve shellfish raising 10~35d in the dechlorination seawater, microalgae biological feed is fed during raising.The cultural method provided by the invention for reducing bivalve shellfish Endophytic bacteria, content of beary metal, bacterial number in Oysters, content of beary metal can be reduced, total number of bacteria and the quantity of coliform reach food sanitation standard, obtained oyster product meat is fertile, without fishy smell, without mucus, it is creamy white.
Description
Technical field
The present invention relates to the technical field of bivalve shellfish purification more particularly to a kind of reduction bivalve shellfish Endophytic bacteria numbers
Amount, the cultural method of content of beary metal.
Background technology
In recent years, marine environmental pollution degree is high, and body eutrophication is serious, frequent occurrence, these environmental factors cause
The quality of bivalve shellfish product reduces.Bivalve shellfish mainly by filter food come in water body of ingesting algae and organic debris.It is natural
Some algae in marine site contain algae toxin, such as dinoflagellate, the red tide algaes such as phaeocystis, while these algae are also enriched heavy metal,
Bivalve shellfish ingests after algae heavy metal and algae toxin all in its cylinder accumulation；The content of organic matter in eutrophication sea area is high,
In a large amount of dissolved organic matters bacterium can be made largely to be proliferated, so that the bacterial number in bivalve shellfish body is increased, these can all make pair
The quality decline of shell shellfish reduces value-added content of product.
The growth of bivalve shellfish is also influenced by many natural causes in mariculture area, such as heavy rain, typhoon, tidewater etc., not
It will produce stress reaction under good environment, and the gland cell meeting secreting mucus in outer embrane meeting protects body, and under freshness
It drops its internal mucus and various protein, lipid etc. and generates trimethylamine, limonene, hexanal under the action of enzyme and microorganism
Deng being made of aldehyde, alcohol, ketone, 4 substance of amine, to generate fishy smell；Further, since bivalve shellfish mainly in water algae and
Planktonic organism be food, the waste with fishy smell that these algae or bacterium generate in metabolic process, gradually in vivo accumulate thus
So that oyster has certain fishy smell, this influences the flavor and taste of bivalve shellfish to a certain extent.In these make
When finished product lists often because coefficient of condition is low, poor quality and influence the quality and selling price of product.In order to improve bivalve shellfish
Edible safety and quality, increase its added value, need before consumption to bivalve shellfish carry out purified treatment.
Traditional purification style is temporarily to support ocean bivalve shellfish in the preferable natural waters of water quality, purification speed it is slow and
It is not thorough.The Chinese invention patent of Patent No. 201410382413.0 (application publication number CN 104145863A) discloses one
The ecological purification method of seed oyster cultivation：Before oyster is put in a suitable place to breed, the sea through net filtration filter is introduced into oyster culture pond Huo Yu Ponds
The probiotics such as microalgae and probiotics are inoculated in water, in 5% ratio of coverage of water by oyster hanging culture in aquaculture pond or fish
In Ponds, reseeding for microalgae and probiotics is carried out every two weeks, and purification is received after 3~4 months, can reduce the ammonia nitrogen that breeding water body summarizes
Etc. harmful substances, increase the dissolved oxygen in water body, improve water quality, improve the getting fat rate of oyster, reduce the heavy metal in Oysters
Content.
The characteristics of above-mentioned purification method is using in microalgae rich in cellulose, after oyster ingests microalgae, weight in alimentary canal
Metal is carried secretly and excreted together by cellulose.However, though above-mentioned purification method can effectively remove the huge sum of money in Oysters
Belong to, but fails to reduce bacterial number in Oysters.
Invention content
The purpose of the present invention is to provide a kind of reduction bivalve shellfish Endophytic bacteria quantity, the cultivation sides of content of beary metal
Method can not only reduce the content of beary metal in bivalve shellfish body, and can reduce the quantity of bivalve shellfish Endophytic bacteria.
In order to achieve the above-mentioned object of the invention, the present invention provides following technical scheme：
The present invention provides a kind of reduction bivalve shellfish Endophytic bacteria quantity, the cultural methods of content of beary metal, including with
Lower step：
1) chlorine-containing disinfectant is carried out mixing 10~14h of disinfection with seawater, be sterilized seawater, the chlorine-containing disinfectant
Available chlorine content is 40~60wt%；The quality of the chlorine-containing disinfectant and the volume ratio of seawater are (600~1350) g:(20~
45)m3；
2) disinfected sea water that the step 1) obtains is mixed with sodium thiosulfate, the mixed solution continuous aeration that will be obtained
After 10~14h, dechlorination seawater is obtained；The mass ratio of the sodium thiosulfate and the disinfected sea water chlorine-containing disinfectant is 1:(1
~3)；
3) by bivalve shellfish raising 10~35d in the dechlorination seawater of the step 2), microalgae biology is fed during raising
Bait.
Preferably, further include heavy metal eccritic in step 3) the dechlorination seawater, the heavy metal eccritic is that dimension is given birth to
Plain C, chitosan, carboxymethyl chitosan or 2,3- dimercaptosuccinic acids.
Preferably, a concentration of 10~15mg/L of the vitamin C in the seawater that dechlorinates；The chitosan is in dechlorination seawater
In a concentration of 10~15mg/L；A concentration of 0.3~0.8mg/L of the carboxymethyl chitosan in the seawater that dechlorinates；Described 2,
A concentration of 0.3~0.8mg/L of the 3- dimercaptosuccinic acids in the seawater that dechlorinates.
Preferably, the method for raising includes in the step 3)：The dechlorination seawater all replaces primary, the feeding daily
The bait for supporting bivalve shellfish is fed 3 times daily.
Preferably, the step 1) chlorine-containing disinfectant includes one or more of strong chlorine oil, bleaching powder and bleaching powder.
Preferably, the bivalve shellfish includes one or more of oyster, clams, scallop and mussel.
Preferably, the bait of step 3) the raising bivalve shellfish is microalgae, concentration of the microalgae in the seawater that dechlorinates
It is 1 × 104~1 × 105Cell/L, the microalgae include one or more of chlorella, egg capsule algae, flat algae and spirulina.
Preferably, the cultural method of the microalgae includes：Microalgae kind is inoculated in micro algae culturing liquid and is cultivated, it is described
The temperature of culture is 22~30 DEG C, and the intensity of illumination of the culture is 1200~2500lux.
Preferably, the dechlorination seawater that described micro algae culturing liquid techniques described above scheme the method uses is solvent, including
The component of following concentration：NaNO370~90mg/L, K2HPO46~10mg/L, FeC6H5O70.1~0.3mg/L, vitamin B11 50
~250 μ g/L, vitamin B120.5~1.5 μ g/L and NaHCO30.5~1.5g/L.
The present invention provides a kind of reduction bivalve shellfish Endophytic bacteria quantity, the cultural methods of content of beary metal, including with
Lower step：1) chlorine-containing disinfectant is carried out mixing 10~14h of disinfection with seawater, be sterilized seawater, the chlorine-containing disinfectant
Available chlorine content is 40~60wt%；The quality of the chlorine-containing disinfectant and the volume ratio of seawater are (600~1350) g:(20~
45)m3；2) disinfected sea water that the step 1) obtains is mixed with sodium thiosulfate, the mixed solution continuous aeration 10 that will be obtained
After~14h, dechlorination seawater is obtained；The mass ratio of the sodium thiosulfate and the disinfected sea water chlorine-containing disinfectant is 1:(1~
3)；3) by bivalve shellfish raising 10~35d in the dechlorination seawater of the step 2), microalgae biological feed is fed during raising.
Chlorine-containing disinfectant provided by the invention carries out disinfection to seawater, can effectively kill bacterium and virus in seawater, reduces bivalve
Micro organism quantity in body of shellfish, and the algae in seawater can be effectively removed, the pollution of prevention and control algae toxin.By daily be oyster more
Disinfected sea water is changed, a breeding environment in good health is provided for oyster, on the one hand greatly reduces the stress reaction of oyster,
On the other hand the generation for greatly reducing fishy smell substance, to which purified oyster meat is creamy white, no mucus, no fishy smell.
The result of the embodiment of the present invention is shown：The reduction bivalve shellfish Endophytic bacteria quantity of offer, the side of content of beary metal
Method, can reduce the content of bacterial number and heavy metal in Oysters, and the quantity of total number of bacteria and coliform reaches edible
Sanitary standard, and oyster product meat is fertile, no mucus, no fishy smell is creamy white.
Further, further include heavy metal eccritic in the dechlorination seawater, the heavy metal eccritic be vitamin C,
Chitosan, carboxymethyl chitosan or 2,3-dimercaptosuccinic acid reduce the content of heavy metal in bivalve shellfish body.
Description of the drawings
Fig. 1 is the quantity of total number of bacteria in 1 Oysters of embodiment；
Fig. 2 is the quantity of coliform in 1 Oysters of embodiment.
Specific implementation mode
The present invention provides a kind of reduction bivalve shellfish Endophytic bacteria quantity, the cultural methods of content of beary metal, including with
Lower step：
1) chlorine-containing disinfectant is carried out mixing 10~14h of disinfection with seawater, be sterilized seawater, the chlorine-containing disinfectant
Available chlorine content is 40~60wt%, and the quality of the chlorine-containing disinfectant and the volume ratio of seawater are (600~1350) g:(20~
45)m3；
2) disinfected sea water that the step 1) obtains is mixed with sodium thiosulfate, the mixed solution continuous aeration that will be obtained
After 10~14h, dechlorination seawater is obtained；The mass ratio of the sodium thiosulfate and the disinfected sea water chlorine-containing disinfectant is 1:(1
~3)；
3) by bivalve shellfish raising 10~35d in the dechlorination seawater of the step 2), microalgae biology is fed during raising
Bait.
In the present invention, the bivalve shellfish includes one or more of oyster, clam, scallop and mussel, described several
Kind is specially two or more.When the bivalve shellfish is two or more, the quantity mixing such as various types of.
In the present invention, the salinity of the seawater be preferably 10~30 ‰, more preferably 15~25 ‰, most preferably 18~
22‰；The pH value of the seawater is preferably 7~9, more preferably 7.8~8.5, most preferably 8.In the present invention, the seawater
It is preferred that first passing through sand filter filtering, reused after being filtered using net filtration；The seawater passes through sand filter, can remove in seawater
Aquatic animal and algae, using net filter can by seawater aquatic animal and algae remove again.The present invention is to described
Sand filter and the specification of net filter are not particularly limited, the specification used using those skilled in the art's conventional filtration seawater.
The present invention carries out chlorine-containing disinfectant with seawater to mix 10~14h of disinfection, and be sterilized seawater；The chlorine-containing disinfecting
The available chlorine content of agent is 40~60wt%, and the quality of the chlorine-containing disinfectant and the volume ratio of seawater are (600~1350) g:
(20~45) m3。
In the present invention, the time that the chlorine-containing disinfectant and seawater mix disinfection is 10~14h, preferably 11~
13h, more preferably 12h.In the present invention, the chlorine-containing disinfectant includes one kind or several in strong chlorine oil, bleaching powder, bleaching powder
Kind, described several specially two or three.When the chlorine-containing disinfectant is two or three, the mass mixings such as each component.
In the present invention, the chlorine-containing disinfectant carries out disinfection to seawater, can effectively kill bacterium and virus in seawater, reduces double
Bacterial number in shell body of shellfish, and the algae in seawater can be effectively removed, the pollution of prevention and control algae toxin.
In the present invention, the available chlorine content of the chlorine-containing disinfectant be 40~60wt%, preferably 45~55wt%, more
Preferably 50wt%.
In the present invention, the quality of the chlorine-containing disinfectant and the volume ratio of seawater are (600~1350) g:(20~45)
m3, preferably (700~1125) g:(25~37.5) m3, more preferably (800~1000) g:(28~32) m3。
The present invention mixes the disinfected sea water with sodium thiosulfate, the 10~14h of mixed solution continuous aeration that will be obtained
Afterwards, dechlorination seawater is obtained；The mass ratio of the sodium thiosulfate and the disinfected sea water chlorine-containing disinfectant is 1:(1~3).
In the present invention, the mass ratio of the sodium thiosulfate and the disinfected sea water chlorine-containing disinfectant is 1:(1~
3), preferably 1:(1.5~2.5), more preferably 1:2.In the present invention, the time of the continuous aeration is 10~14h, preferably
For 11~13h, more preferably 12h.In the present invention, the sodium thiosulfate and aeration can remove in the seawater after disinfection
Chlorine.
Bivalve shellfish is raised 10~35d in the seawater that dechlorinates by the present invention.
In the present invention, when the bivalve shellfish is preferably oyster, the oyster is preferably Hong Kong oyster, the oyster
Quality be preferably 125~150g/ only, more preferably 130~140g/ only.
The present invention by bivalve shellfish raise dechlorination seawater time be 10~35d, preferably 15~30d, more preferably
20~25d.
In the present invention, the bait of the raising bivalve shellfish is preferably microalgae, and the microalgae is dense in the seawater that dechlorinates
Degree preferably 1 × 104~5Cell/L.In the present invention, the microalgae includes one in chlorella, egg capsule algae, flat algae and spirulina
Kind is several.When the microalgae is preferably chlorella and egg capsule algae, the cell quantity ratio of the chlorella and egg capsule algae is preferred
For (5~15):1, more preferably (8~12):1, most preferably 10:1；When the microalgae is preferably spirulina and egg capsule algae,
The spirulina and the cell quantity ratio of egg capsule algae are preferably (0.5~1.5):(0.5~1.5), more preferably (0.8~1.2):
(0.8~1.2), most preferably 1:1.In the present invention, the microalgae is not only that bivalve shellfish provides sufficient bait, is improved
The getting fat rate of bivalve shellfish, and microalgae can be combined to remove removing heavy metals with heavy metal.In the present invention.By feeding cleaning
Bait micro-algae, reduce the content of beary metal in Oysters, effective prevention and control algae toxin pollution, and effectively increase oyster
Coefficient of condition.
In the present invention, the cultural method of the microalgae kind preferably includes：By microalgae kind be inoculated in micro algae culturing liquid into
Row culture, the present invention is not particularly limited the inoculum concentration of the microalgae kind, using those skilled in the art's routine microalgae kind
Inoculum concentration.In the present invention, the temperature of the culture is preferably 22~30 DEG C, more preferably 25~28 DEG C；The culture
Intensity of illumination be preferably 1200~2500lux, more preferably 1500~2000lux, most preferably 1800lux.The present invention couple
The time of the microdisk electrode is not particularly limited, using the time of those skilled in the art's routine culture microalgae.
In the present invention, the dechlorination seawater described in the micro algae culturing liquid techniques described above scheme is solvent, is preferably included
The component of following concentration：NaNO370~90mg/L, K2HPO46~10mg/L, FeC6H5O70.1~0.3mg/L, vitamin B11 50
~250 μ g/L, vitamin B120.5~1.5 μ g/L and NaHCO30.5~1.5g/L；More preferably include：NaNO375~85mg/L,
K2HPO47~9mg/L, FeC6H5O70.15~0.25mg/L, 80~220 μ g/L of vitamin B11, vitamin B120.8~1.2 μ g/
L and NaHCO30.8~1.2g/L；Most preferably include：NaNO380mg/L、K2HPO48mg/L、FeC6H5O70.2mg/L, vitamin
B1200 μ g/L, vitamin B121.0 μ g/L and NaHCO31.0g/L。
In the present invention, the method for the raising includes：The dechlorination seawater is preferably all replaced primary daily；The feeding
The bait for supporting bivalve shellfish is preferably fed 3 times daily, in embodiments of the present invention specifically 7:00、15:00 and 22:00 respectively into
Row is fed.
In the present invention, the dechlorination seawater preferably further includes heavy metal eccritic.
In the present invention, the heavy metal eccritic is vitamin C, chitosan, carboxymethyl chitosan or 2,3- dimercaptos
Succinic acid；Concentration of the vitamin C in the seawater that dechlorinates is preferably 10~15mg/L, more preferably 11.5~13mg/L, most
Preferably 12.5mg/L；Concentration of the chitosan in the seawater that dechlorinates be preferably 10~15mg/L be more preferably 11.5~
13mg/L, most preferably 12.5mg/L；Concentration of the carboxymethyl chitosan in the seawater that dechlorinates is preferably 0.3~0.8mg/L,
More preferably 0.4~0.6mg/L, most preferably 0.5mg/L；Concentration of the 2,3- dimercaptosuccinic acids in the seawater that dechlorinates is excellent
It is selected as 0.3~0.8mg/L, more preferably 0.4~0.6mg/L, most preferably 0.5mg/L.In the present invention, the heavy metal promotees
Row's agent is added in dechlorination seawater, eccritic and metal ion in conjunction with and excluded in vitro.By adding a huge sum of money in the present invention
Belonging to eccritic effectively reduces the content of beary metal in bivalve shellfish body.
With reference to embodiment a kind of bivalve shellfish Endophytic bacteria quantity, content of beary metal are reduced to provided by the invention
Cultural method is described in detail, but they cannot be interpreted as limiting the scope of the present invention.
Embodiment 1
It is 2 × 25m to specification2After indoor cement pit disinfection, the seawater for passing through sand filter and net filter successively is added into 1.8m,
It is that 50% strong chlorine oil 1350g sterilizes 12h to seawater body row, then is neutralized with 675g sodium thiosulfate, continuous aeration with effective chlorine
12h waits for that chlorine residue all disappears in water, obtains dechlorination seawater.Dechlorination seawater is introduced into 0.5m through net filter3PVC buckets in, each
The introduction volume of seawater is 0.3m in bucket3, 2 cylinder moulds are hung in each bucket, and 20 contaminated oysters are filled in each cylinder mould.5
A processing group is respectively A, B, C, D, E；A, the PVC buckets of B, C group introduce dechlorination seawater, and the PVC buckets of D, E group introduce the sea that do not dechlorinate
Water；A groups replace the seawater that do not dechlorinate daily, and B groups replace the seawater that do not dechlorinate every other day, and C groups every other day replace dechlorination seawater, and D groups are every
It replaces dechlorination seawater, and E groups replace dechlorination seawater every three days, and each processing setting 3 is parallel.In natural light and at room temperature into
The pH of row, seawater is 8, and salinity is 20 ‰, feeding time 10d.The daily clean spirulina for periodically feeding culture 3 times, often
Secondary feeding volume keeps the concentration of the spirulina in water body 2 × 104Cell/L.It takes out and deposits at random from each bucket respectively daily
Oyster living, opens shell, its soft body is taken to survey the measurement with coliform for total number of bacteria, as a result such as Fig. 1 and Fig. 2.
The cultural method of spirulina includes spirulina being inoculated in micro algae culturing liquid according to traditional vaccination amount, in temperature
It it is 25 DEG C, intensity of illumination is to cultivate spirulina under 1200lux；Micro algae culturing liquid is using the seawater that dechlorinates as solvent, including following concentration
Component：NaNO380mg/L、K2HPO48mg/L、FeC6H5O70.2mg/L, 00 μ g/L of vitamin B12, vitamin B121.0μg/L
And NaHCO31.0g/L。
As shown in Figure 1, by the raising of 10d, the quantity of total number of bacteria is in downward trend in each group Oysters, and D groups are
It is minimum that total number of bacteria quantity in the Oysters of dechlorination seawater is replaced daily.As shown in Figure 2, in each group Oysters coliform number
Amount is continuously decreased with raising, and D groups are that the daily quantity for replacing coliform in dechlorination seawater Oysters is minimum.By 10d
Raising, total number of bacteria and the quantity of coliform reach food sanitation standard in Oysters.The oyster production obtained after purification
Product meat is fertile, no mucus, and no fishy smell is creamy white.
Embodiment 2
It is 1.5 × 25m to specification2After indoor cement pit disinfection, the seawater for passing through sand filter and net filter successively is added to
0.8m is that 50% strong chlorine oil 600g sterilizes 12h to water body row, then is neutralized with 300g sodium thiosulfate, continuous aeration with effective chlorine
12h waits for that chlorine residue all disappears in water, obtains dechlorination seawater.Dechlorination seawater is introduced into 15 0.5m through net filter3PVC buckets in,
The introduction volume of disinfected sea water is 0.3m in each bucket3, 2 cylinder moulds are hung in each bucket, fill in each cylinder mould 20 it is contaminated
Oyster, replace breeding water body daily.The salinity of 5 processing groups is respectively 10 ‰, 15 ‰, 20 ‰, 25 ‰ and 30 ‰, salinity
20 ‰ as a control group, and each processing setting 3 is parallel.It carries out in natural light and at room temperature, the pH of seawater is 8, feeding time
For 35d.The daily clean spirulina for periodically feeding culture 3 times, each feeding volume keeps the concentration of the spirulina in water body
2 × 104Cell/L.The oyster for taking out survival at random from each bucket respectively in 0d, 7d, 14d, 21d, 28d and 35d, opens shell,
Take its soft body for the measurement of heavy metal Cu, Cd, as a result such as table 1-2.
The cultural method of the present embodiment spirulina according to 1 spirulina of embodiment cultural method.
Content (the unit of copper in 1 Oysters of table：mg/kg)
| Salinity | 0d | 7d | 14d | 21d | 28d | 35d |
| 30‰ | 813.43 | 697.95 | 576.53 | 542.22 | 469.22 | 419.90 |
| 25‰ | 778.01 | 629.79 | 552.11 | 520.33 | 451.65 | 420.45 |
| 20‰ | 750.93 | 670.70 | 559.24 | 491.23 | 465.35 | 443.84 |
| 15‰ | 725.21 | 658.84 | 608.91 | 334.71 | 444.28 | 406.18 |
| 10‰ | 757.21 | 667.06 | 568.00 | 520.09 | 474.49 | 434.55 |
As can be drawn from Table 1, when salinity is 30 ‰, the rate of discharge of copper is maximum, is 39.84%；When salinity is 15 ‰,
The rate of discharge of copper is also larger, is 38.35%.
Content (the unit of cadmium in 2 Oysters of table：mg/kg)
| Salinity | 0d | 7d | 14d | 21d | 28d | 35d |
| 30‰ | 8.24 | 7.08 | 6.91 | 6.14 | 5.42 | 4.44 |
| 25‰ | 8.22 | 6.86 | 6.29 | 5.89 | 5.28 | 4.71 |
| 20‰ | 7.62 | 6.29 | 6.22 | 5.82 | 5.51 | 4.71 |
| 15‰ | 8.70 | 7.33 | 5.72 | 5.41 | 5.08 | 3.91 |
| 10‰ | 7.65 | 6.88 | 6.46 | 5.60 | 5.39 | 4.30 |
As can be drawn from Table 2, when salinity is 15 ‰, the rate of discharge of cadmium is maximum, is 55.08%.At the purification of 35d
Reason, oyster product meat is fertile, no mucus, and no fishy smell is creamy white.
Embodiment 3
It is 1.8 × 25m to specification2After indoor cement pit disinfection, the seawater for passing through sand filter and net filter successively is added to
1.5m is that 50% strong chlorine oil 1125g sterilizes 12h to water body row, then is neutralized with 562.5g sodium thiosulfate with effective chlorine, continuous to expose
Gas 12h waits for that chlorine residue all disappears in water, obtains dechlorination seawater.Dechlorination seawater is introduced into 18 0.6m through net filter3PVC buckets
In, the introduction volume for the seawater that dechlorinates in each bucket is 0.4m3, 2 cylinder moulds are hung in each bucket, filling 20 in each cylinder mould gets dirty
The oyster of dye, replaces breeding water body daily.The bait of 5 processing groups is respectively spirulina, egg capsule algae, chlorella, chlorella+ovum
Capsule algae, spirulina+egg capsule algae, control group do not feed bait, and each processing setting 3 is parallel.It carries out in natural light and at room temperature,
The pH of seawater is 8, feeding time 35d.The daily cleaning bait micro-algae for periodically feeding culture 3 times, cleans the throwing of bait every time
The amount of feeding keeps the concentration of frond in water body 2 × 104Cell/L.In 0d, 7d, 14d, 21d, 28d and 35d respectively from each bucket
The random oyster for taking out survival, opens shell, takes its soft body for the measurement of heavy metal Cu, Cd, as a result such as table 3~4.
The cultural method of the present embodiment spirulina, egg capsule algae and chlorella according to 1 spirulina of embodiment cultural method.
The cell quantity ratio of chlorella and egg capsule algae is 10:1, the cell quantity ratio of spirulina and egg capsule algae is 1:1.
Content (the unit of copper in 3 Oysters of table：mg/kg)
Content (the unit of cadmium in 4 Oysters of table：mg/kg)
It can be obtained by table 3~4, spirulina, egg capsule algae, chlorella, mixed feed group can effectively promote copper, cadmium
Discharge, with control group have conspicuousness.Mixed algae (chlorella+egg capsule algae) promotes the best results of heavy metal discharge, discharge
The rate of copper is 53.47%, and the rate that cadmium is discharged is 58.46%, is secondly spirulina.Purified treated oyster product meat
Matter is fertile, no mucus, and no fishy smell is creamy white.
Embodiment 4
It is 1.5 × 25m to specification2After indoor cement pit disinfection, the seawater for passing through sand filter and net filter successively is added into 1m,
It is that 50% strong chlorine oil 750g sterilizes 12h to water body row, then is neutralized with 375g sodium thiosulfate with effective chlorine, continuous aeration 12h is waited for
Chlorine residue all disappears in water, obtains dechlorination seawater.Dechlorination seawater is introduced into 15 1m3PVC buckets in, in each bucket dechlorinate sea
The introduction volume of water is 0.7m3.Heavy metal eccritic is added in dechlorination, 3 cylinder moulds of hanging in each bucket, in each cylinder mould
20 contaminated oysters are filled, replace dechlorination seawater daily.The heavy metal eccritic of 5 processing groups is respectively Vc (12.5mg/
L), chitosan (12.5mg/L), carboxymethyl chitosan (0.5mg/L), 2,3-dimercaptosuccinic acid (0.5mg/L), control group is not
Heavy metal eccritic is added, each processing setting 3 is parallel.It carries out in natural light and at room temperature, the pH of seawater is 8, when raising
Between be 35d.The daily cleaning spirulina for periodically feeding culture 3 times, each feeding volume keeps the concentration of the spirulina in water body
1~2 × 104Cell/L.The oyster for taking out survival at random from each bucket respectively in 0d, 7d, 14d, 21d, 28d and 35d, opens
Shell takes its soft body for the measurement of heavy metal Cu, Cd, as a result such as table 5~6.
Content (the unit of copper in 5 Oysters of table：mg/kg)
Content (the unit of cadmium in 6 Oysters of table：mg/kg)
By table 5~6 it is found that each heavy metal eccritic can be effectively facilitated the discharge of heavy metal, have compared with the control group
Significant, for 2,3-dimercaptosuccinic acid to the discharge best results of copper and cadmium, discharge rate is respectively 59.85%, 51.32%.
The oyster product meat obtained after purified is fertile, and no mucus, no fishy smell is creamy white.
As seen from the above embodiment, the present invention provides the method for the reduction bivalve shellfish Endophytic bacteria content of offer, energy
The quantity of the enough quantity of reduction oyster Endophytic bacteria, total number of bacteria and coliform reaches food sanitation standard.After purification
The oyster product meat arrived is fertile, and no mucus, no fishy smell is creamy white.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (9)
1. a kind of reduction bivalve shellfish Endophytic bacteria quantity, the cultural method of content of beary metal, include the following steps：
1) chlorine-containing disinfectant and seawater are carried out mixing 10~14h of disinfection, be sterilized seawater, the chlorine-containing disinfectant it is effective
Chlorinity is 40~60wt%；The quality of the chlorine-containing disinfectant and the volume ratio of seawater are (600~1350) g:(20~45)
m3；
2) disinfected sea water that the step 1) obtains is mixed with sodium thiosulfate, by obtained mixed solution continuous aeration 10~
After 14h, dechlorination seawater is obtained；The mass ratio of the sodium thiosulfate and the disinfected sea water chlorine-containing disinfectant is 1:(1~
3)；
3) by bivalve shellfish raising 10~35d in the dechlorination seawater of the step 2), microalgae biological feed is fed during raising.
2. according to the cultural method described in right 1, which is characterized in that further include heavy metal decorporation in step 3) the dechlorination seawater
Agent, the heavy metal eccritic are vitamin C, chitosan, carboxymethyl chitosan or 2,3-dimercaptosuccinic acid.
3. cultural method according to claim 2, which is characterized in that the vitamin C is a concentration of in the seawater that dechlorinates
10~15mg/L；A concentration of 10~15mg/L of the chitosan in the seawater that dechlorinates；The carboxymethyl chitosan is in dechlorination sea
A concentration of 0.3~0.8mg/L in water；A concentration of 0.3~0.8mg/ of the 2,3- dimercaptosuccinic acids in the seawater that dechlorinates
L。
4. cultural method according to claim 1, which is characterized in that the method for raising includes in the step 3)：It is described
The seawater that dechlorinates all is replaced once daily, and the bait of the raising bivalve shellfish is fed 3 times daily.
5. cultural method according to claim 1, which is characterized in that the step 1) chlorine-containing disinfectant include strong chlorine oil,
The one or more of bleaching powder and bleaching powder.
6. cultural method according to claim 1, which is characterized in that the bivalve shellfish include oyster, clams, scallop and
One or more of mussel.
7. cultural method according to claim 1, which is characterized in that the bait of step 3) the raising bivalve shellfish is micro-
Algae, the microalgae in the seawater that dechlorinates a concentration of 1 × 104~1 × 105Cell/L, the microalgae include chlorella, egg capsule algae,
One or more of flat algae and spirulina.
8. cultural method according to claim 7, which is characterized in that the cultural method of the microalgae includes：By microalgae kind
It is inoculated in micro algae culturing liquid and is cultivated, the temperature of the culture is 22~30 DEG C, and the intensity of illumination of the culture is 1200
~2500lux.
9. cultural method according to claim 8, which is characterized in that the micro algae culturing liquid is with side described in claim 1
The dechlorination seawater that method uses is solvent, includes the component of following concentration：NaNO370~90mg/L, K2HPO46~10mg/L,
FeC6H5O70.1~0.3mg/L, 150~250 μ g/L of vitamin B1, vitamin B120.5~1.5 μ g/L and NaHCO3 0.5
~1.5g/L.
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| CN115152674A (en) * | 2022-08-16 | 2022-10-11 | 黄晓菊 | Method for breeding oysters capable of being eaten raw |
| CN116262672A (en) * | 2023-02-20 | 2023-06-16 | 辽宁省海洋水产科学研究院(辽宁省农业科学院大连生物技术研究所、辽宁省海洋环境监测总站) | Preparation method of sodium alginate composite slow-release phosphate fertilizer, product and application thereof |
| CN116262672B (en) * | 2023-02-20 | 2023-10-27 | 辽宁省海洋水产科学研究院(辽宁省农业科学院大连生物技术研究所、辽宁省海洋环境监测总站) | Preparation method of sodium alginate composite slow-release phosphate fertilizer, product and application thereof |
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