CN102730909A - Preparation method of seawater for field shellfish unicellular alga culture - Google Patents
Preparation method of seawater for field shellfish unicellular alga culture Download PDFInfo
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- CN102730909A CN102730909A CN2012102082331A CN201210208233A CN102730909A CN 102730909 A CN102730909 A CN 102730909A CN 2012102082331 A CN2012102082331 A CN 2012102082331A CN 201210208233 A CN201210208233 A CN 201210208233A CN 102730909 A CN102730909 A CN 102730909A
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- seawater
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- unit cell
- cell algae
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
Abstract
The invention relates to a preparation method of seawater for field shellfish unicellular alga culture. The preparation method comprises the steps of seawater precipitation, filter feeding shellfish filter, absorbent cotton filter and disinfection. The preparation method, which utilizes particulate substance filter physiological characteristics of seawater bivalve shellfish and allows filter materials of a nylon net, the absorbent cotton and the like to be cooperatively used, enables a large amount of clear seawater to be prepared without a sand filter tank, so the preparation method has the advantages of low cost and convenient operation, can be widely applied to field experiments or small-scale seedling production, and has a good application prospect.
Description
Technical field
The invention belongs to the cultivation field of open-air shellfish unit cell algae, particularly a kind of open-air shellfish unit cell algae is cultivated the preparation method with seawater.
Background technology
Unicellular algae is the high-quality bait of filter-feeding shellfish, is widely used in shellfish hatchery and breed.At present, the culture technique of unit cell algae is ripe, and one will carry out three grades of cultivations, that is: protect and expand kind of a cultivation, enlarged culturing and production cultivation.During the unit cell algae was cultivated, except that illumination, nutritive salt and temperature, the processing of water was an important link.Filtration and sterilization comprising water.Protecting kind of water generally needs through the secondary sand filtration, and then filters through gac or absorbent cotton, and then carries out scalding or sterilized water sterilization.In algae enlarged culturing and production were cultivated, used seawater also needed through sand filtration.But, in the open air the small-scale shellfish hatchery or scientific research and testing, receive place or condition restriction, often can't obtain the sand filtration seawater.If single not only consuming time, the gained water yield is limited with absorbent cotton or activated carbon filtration, and absorbent cotton and gac replacing at any time, cumbersome, cost is also higher.In addition, its filter effect is also undesirable, especially for mud type mudflat seawater, filters the back water body and contains a large amount of suspended particulates, and light transmission is poor, and the growth of unit cell algae is cultivated in influence.
The filter-feeding marine shellfish can be filtered plant plankton and the particulate matter in the food picked-up seawater, and filtration rate is high, Zhang Jihong etc. (2005) report, and the per hour filtering seawater amount of single chlamys farreri is up to 4L.Only selective when the filter-feeding shellfish ingests to the size and the quality of food, and to whether having edibleness not have selectivity.Therefore, the particle in the certain grain size scope that the filter-feeding shellfish suspends in can filtering sea, and false excrement of it formation or ight soil entered in the substrate.According to
(1988) research report, bivalve shellfish is all filterings of particle more than the 4-5 μ m to particle diameter; To the particle of particle diameter 2 μ m, its rejection rate also can reach 50%.And report that according to the research of (2007) such as Li Zhanhai the outstanding husky particle diameter compositing range of tidal flat is between 3-22 μ m.Therefore, this physiological property of ingesting of bivalve filter food shellfish can be used.
Summary of the invention
Technical problem to be solved by this invention provides a kind of open-air shellfish unit cell algae and cultivates the preparation method with seawater; This method is utilized the physiological property of seawater bivalve shellfish filter particulate material, cooperates filtering materials such as nylon wire, absorbent cotton, need not sand filter and can prepare a considerable amount of than ten clean seawater; Cost is low; Easy and simple to handle, can be widely used in field trial or the production of growing seedlings on a small scale, have a good application prospect.
A kind of open-air shellfish unit cell algae of the present invention is cultivated the preparation method with seawater, comprising:
(1) with precipitating in seawater extracting to the filtration vessel, the filtration vessel top hides with black cloth, and the ST is 24～28 hours; Extract settling tank top seawater to filtration vessel, pump outlet carries out prefiltration with nylon net bag to seawater;
(2) select healthy filter-feeding shellfish and be placed in the net, put into the filtration vessel bottom then, filtration vessel hides with black cloth, and is every at a distance from sampling observation in 6 hours, clarifies to see the bottom and gets final product to estimating water body; Filtration finishes, and removes shellfish ight soil and settling on bottom and the net cage with the flexible pipe siphon;
(3) carry out scalding to being used for unit cell algae guarantor kind of a seawater of cultivating, use sodium hypochlorite solution or chlorinated lime to carry out disinfection with the seawater of producing cultivation being used for unit cell algae enlarged culturing; After sterilization finishes, inflate after 12 ~ 16 hours with in the Sulfothiorine and after promptly can be used for the unit cell algae and inoculate.
Filtration vessel in the said step (1) is indoor settling tank or Glass fibre reinforced plastic tub.
The order number of the nylon net bag in the said step (1) is the 150-200 order.
Filter-feeding shellfish in the said step (2) is a clam.
Use the absorbent cotton filtering sea to being used for before the unit cell algae protects kind of the seawater scalding of cultivating in the said step (3), remove large granular impurity.
Said absorbent cotton filters concrete technology: the absorbent cotton of thickness 2-3cm is tiled in the B, filters to funnel with small hose siphon seawater.
The concentration of the Sulfothiorine in the said step (3) is 25ppm.
Beneficial effect
The present invention utilizes the physiological property of seawater bivalve shellfish filter particulate material; Cooperate filtering materials such as nylon wire, absorbent cotton; Need not sand filter and can prepare a considerable amount of clean seawater, cost is low, and is easy and simple to handle; Can be widely used in field trial or the production of growing seedlings on a small scale, have a good application prospect.
Embodiment
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment only to be used to the present invention is described and be not used in the restriction scope of the present invention.Should be understood that in addition those skilled in the art can do various changes or modification to the present invention after the content of having read the present invention's instruction, these equivalent form of values fall within the application's appended claims institute restricted portion equally.
Embodiment 1
Protect kind of a cultivation:
1, seawater deposition
Used seawater is taken from the outdoor water reservoir of 100m * 100m, with small-sized submersible pump with the seawater extracting to indoor cement settling tank, the 1.5m of the long 4m of settling tank specification * wide 3m * deeply, water pump water outlet mouthpiece cover 150 purpose nylon net bags carry out prefiltration, add water to 1.2m.Settling tank is used the black cloth shading, precipitates after 24 hours, submersible pump is placed on one filters in the net cage, and net cage is put into settling tank, in the white plastic tank with 2 100L of seawater suction, and the high 1m of bucket.
2, the filter-feeding shellfish is filtered
Buy about clam 5kg common on the market, the average long 4.5cm of shell cleans shell, on average puts into 2 plastics string bags, puts into white plastic tank then respectively.Inflation hides with black cloth in the bucket.Whenever observe at a distance from sampling in 4 hours, remove the bucket end, online shellfish ight soil and settling with the flexible pipe siphon simultaneously; After 12 hours, the visible bucket of the range estimation end, the plastics string bag is taken out.
3, absorbent cotton filters
Get one of B (go up bore 150mm, bore 3cm lets slip a remark), absorbent cotton is layered in the funnel layer by layer, compress, to thickness 3cm, spread to the funnel edge on both sides; The with cover round plastic tank of 50L, top cover boring, aperture 3.5 – 4cm.Plastic cylinder is placed on the lower, and funnel places the hole of bung, filters in siphon seawater to the funnel in above-mentioned white plastic tank with small hose, controls water flow velocity in case overflow with clip, completely can stop to filter to bucket.
4, seawater sterilization
Get the Erlenmeyer flask of 5000mL, scrub, after the fresh water flushing, add the filtering seawater of absorbent cotton to 4500mL with 1:1 hydrochloric acid.After boiling with the 1500W electric furnace, seal with double-deck printer paper, and tighten with bungee.Promptly can be used for inoculation after cooling.
5, inoculation culture
Isochrysis galbana and each 500mL of Chlorella pyrenoidesa algae kind are inserted in the 4500 good seawater of scalding, add nutrient salt solution respectively, place indoor cultivation, adopt natural lighting, room temperature 26-33 ℃.Shake bottle every day 4 times.To the 6th day, algae liquid concentration reached 2.3 * 10 respectively
6Individual/ml and 2.5 * 10
6Individual/ml.And the same period is only obviously lighter with two kinds of algae colors that the absorbent cotton filtering sea is cultivated, and mean concns is respectively 1.4 * 10
6Individual/ml and 1.5 * 10
6Individual/ml, and poor growth, wherein chrysophyceae is influenced particularly evident.
Embodiment 2
Enlarged culturing:
1, seawater extracting and deposition
Used seawater is taken from the outdoor water reservoir of 100m * 100m, with small-sized submersible pump with the seawater extracting to indoor cement settling tank, the 1.5m of the long 4m of settling tank specification * wide 3m * deeply, water pump water outlet mouthpiece cover 200 purpose nylon net bags carry out prefiltration, add water to 1.2m.Settling tank is used the black cloth shading, precipitates after 28 hours, submersible pump is placed on one filters in the net cage, and net cage is put into one jiao of settling tank, in the white plastic cylinder with 15 100L of seawater suction, and the high 1m of bucket.
2, the filter-feeding shellfish is filtered
Buy clam, the average long 4.5cm of shell cleans shell, puts into the plastics string bag, puts into each white plastic tank respectively, puts about 2.5kg for every barrel.Inflation hides with black cloth in the bucket; Whenever observe at a distance from sampling in 4 hours, remove the bucket end, online shellfish ight soil and settling with the flexible pipe siphon simultaneously.After 12 hours, the visible bucket of the range estimation water body end, the plastics string bag to be taken out, this moment, clam can be used for sea water filter next time.
3, seawater sterilization
Sodium hypochlorite solution (available chlorine 15-30%) splashed in the bucket by the concentration of 250ppm carry out disinfection; Inflation makes the thimerosal uniform distribution in the bucket; Simultaneously the transparent plastic film of a 1m * 1m is put into the bucket sterilization; Add the Sulfothiorine neutralization after 16 hours, concentration is controlled at about 25ppm, gets final product inoculation culture after stirring, inflating.
4, inoculation culture
In the seawater that the chrysophyceae that protects kind and chlorella are disinfected in the ratio access of 1:8, add nutrient salt solution respectively, cover bung, carry out enlarged culturing with disinfectant transparent plastic film.During cultivation, with the little inflation of independent air pump or stir every day 3 times.Under the competent condition of illumination, algae liquid concentration all reaches 2.0 * 10 after 5 days
6Individual/as more than the ml, to can be used for testing throwing something and feeding of shellfish.
Claims (7)
1. an open-air shellfish unit cell algae is cultivated the preparation method with seawater, comprising:
(1) with precipitating in seawater extracting to the filtration vessel, the filtration vessel top hides with black cloth, and the ST is 24 ~ 28 hours; Extract settling tank top seawater to filtration vessel, pump outlet carries out prefiltration with nylon net bag to seawater;
(2) select healthy filter-feeding shellfish and be placed in the net, put into the filtration vessel bottom then, filtration vessel hides with black cloth, and is every at a distance from sampling observation in 6 hours, clarifies to see the bottom and gets final product to estimating water body; Filtration finishes, and removes shellfish ight soil and settling on bottom and the net cage with the flexible pipe siphon;
(3) carry out scalding to being used for unit cell algae guarantor kind of a seawater of cultivating, use sodium hypochlorite solution or chlorinated lime to carry out disinfection with the seawater of producing cultivation being used for unit cell algae enlarged culturing; After sterilization finishes, inflate after 12 ~ 16 hours with in the Sulfothiorine and after promptly can be used for the unit cell algae and inoculate.
2. a kind of open-air shellfish unit cell algae according to claim 1 is cultivated the preparation method with seawater, and it is characterized in that: the filtration vessel in the said step (1) is indoor settling tank or Glass fibre reinforced plastic tub.
3. a kind of open-air shellfish unit cell algae according to claim 1 is cultivated the preparation method with seawater, and it is characterized in that: the order number of the nylon net bag in the said step (1) is the 150-200 order.
4. a kind of open-air shellfish unit cell algae according to claim 1 is cultivated the preparation method with seawater, and it is characterized in that: the filter-feeding shellfish in the said step (2) is a clam.
5. a kind of open-air shellfish unit cell algae according to claim 1 is cultivated the preparation method with seawater, it is characterized in that: use the absorbent cotton filtering sea to being used for before the unit cell algae protects kind of the seawater scalding of cultivating in the said step (3), remove large granular impurity.
6. a kind of open-air shellfish unit cell algae according to claim 1 is cultivated the preparation method with seawater; It is characterized in that: said absorbent cotton filters concrete technology and is: the absorbent cotton of thickness 2-3cm is tiled in the B, filters to funnel with small hose siphon seawater.
7. a kind of open-air shellfish unit cell algae according to claim 1 is cultivated the preparation method with seawater, and it is characterized in that: the concentration of the Sulfothiorine in the said step (3) is 25ppm.
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| CN201210208233.1A CN102730909B (en) | 2012-06-21 | 2012-06-21 | Preparation method of seawater for field shellfish unicellular alga culture |
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| CN201210208233.1A CN102730909B (en) | 2012-06-21 | 2012-06-21 | Preparation method of seawater for field shellfish unicellular alga culture |
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| CN102730909A true CN102730909A (en) | 2012-10-17 |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104872045A (en) * | 2015-06-01 | 2015-09-02 | 邹苏琪 | Fully-automatic juvenile fish breeding device |
| CN108668965A (en) * | 2018-03-22 | 2018-10-19 | 广东海洋大学 | A kind of reduction bivalve shellfish Endophytic bacteria quantity, the cultural method of content of beary metal |
| CN109336285A (en) * | 2018-10-23 | 2019-02-15 | 江苏省海洋水产研究所 | A kind of easy preparation device and its application method of microalgae conservation culture water |
| CN109769685A (en) * | 2019-02-03 | 2019-05-21 | 福安市冠富农业科技有限公司 | A kind of culture zone scene spreads cultivation algae method |
| CN109796092A (en) * | 2019-02-03 | 2019-05-24 | 福安市冠富农业科技有限公司 | A kind of method for purifying seawater |
| CN113080051A (en) * | 2021-03-26 | 2021-07-09 | 厦门海石生态环境股份有限公司 | Method for promoting recovery of shallow sea seaweed bed |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101156558A (en) * | 2007-10-19 | 2008-04-09 | 中国水产科学研究院南海水产研究所 | large nacre artificial sprout continental rise controlled water body intermediate cultivate method |
-
2012
- 2012-06-21 CN CN201210208233.1A patent/CN102730909B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101156558A (en) * | 2007-10-19 | 2008-04-09 | 中国水产科学研究院南海水产研究所 | large nacre artificial sprout continental rise controlled water body intermediate cultivate method |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104872045A (en) * | 2015-06-01 | 2015-09-02 | 邹苏琪 | Fully-automatic juvenile fish breeding device |
| CN108668965A (en) * | 2018-03-22 | 2018-10-19 | 广东海洋大学 | A kind of reduction bivalve shellfish Endophytic bacteria quantity, the cultural method of content of beary metal |
| CN109336285A (en) * | 2018-10-23 | 2019-02-15 | 江苏省海洋水产研究所 | A kind of easy preparation device and its application method of microalgae conservation culture water |
| CN109769685A (en) * | 2019-02-03 | 2019-05-21 | 福安市冠富农业科技有限公司 | A kind of culture zone scene spreads cultivation algae method |
| CN109796092A (en) * | 2019-02-03 | 2019-05-24 | 福安市冠富农业科技有限公司 | A kind of method for purifying seawater |
| CN113080051A (en) * | 2021-03-26 | 2021-07-09 | 厦门海石生态环境股份有限公司 | Method for promoting recovery of shallow sea seaweed bed |
| CN113080051B (en) * | 2021-03-26 | 2023-10-20 | 厦门海石生态环境股份有限公司 | Method for promoting recovery of shallow sea seaweed bed |
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