CN104872045A - Fully-automatic juvenile fish breeding device - Google Patents

Abstract

The invention relates to a fully-automatic juvenile fish breeding device which comprises three parts, namely a round siphoning cylinder, a paramecium propagation cylinder and a water-circulation filtering system, wherein the round siphoning cylinder is connected with the paramecium propagation cylinder by virtue of a feeding pipe; the water circulation filtering system is capable of recycling fish culture water, which overflows from the round siphoning cylinder, by virtue of a diversion trench; residual fish culture water in the round siphoning cylinder overflows to the water circulation filtering system by virtue of an inverted U-shaped pipe. <0}{0><}0{>The fully-automatic juvenile fish breeding device can be used for fully-automatic breeding during a juvenile fish stage after incubation of zebra fish, medaka and the like.

Description

A kind of automatic rearing device for juvenile fish

technical field

The invention relates to a juvenile fish feeding device, which belongs to the technical field of fish feeding, and in particular relates to automatic feeding of juvenile fish fed on paramecium in laboratories and commercial applications.

Background technique

Model animals such as zebrafish and medaka belong to lower vertebrates, and they have the advantages of fast development, large number of offspring, low feeding cost, and small footprint. It has a wide range of applications in life sciences, environmental sciences, etc. At present, there are more and more laboratories around the world that use these two fishes as research objects. However, the rearing of juvenile fish has the disadvantages of heavy labor and low work efficiency. In the current laboratory breeding environment, they are usually kept in petri dishes, changing water and feeding every day seriously wastes the precious time of scientific researchers. Especially in the aspects of mutant screening and transgenic strain construction, there is an urgent need for a simple, time-saving and efficient device for rearing juvenile fish.

Contents of the invention

In order to improve the feeding efficiency of juvenile fish and reduce the time for manual water change and feeding, the present invention provides a device for automatically feeding juvenile fish.

The present invention is realized through the following technical scheme. The juvenile fish automatic feeding device includes three parts: a siphon cylinder, a paramecium breeding tank and a water circulation filtration system. The siphon cylinder and the paramecia breeding tank are connected through a feeding pipe, and the water circulation The filter system recovers the fish culture water overflowed from the siphon cylinder through the diversion groove, and the excess fish culture water overflowed from the siphon cylinder to the water circulation filter system through the inverted "U" pipe.

Described siphon cylinder comprises juvenile fish chamber and buffer chamber, also comprises vertical pipe and inverted " U " pipe, also has a shading cover, and a circular screen is arranged between juvenile fish chamber and vertical pipe. The juvenile fish room is the main place for rearing juvenile fish. The buffer room is a space for buffering the overflow of zebrafish. The juvenile fish room and the buffer room are connected through vertical pipes. The shading cover is a light-proof cylinder that wraps the buffer room. Connects with inverted "U" tubing.

The paramecium breeding tank includes a flour fermentation room and a paramecium breeding room, with an isolation net and a water level control valve, as well as a paramecium feeding pipe and a pump. The flour fermentation room is a space separated by an isolation net in the paramecium breeding room, the water level control valve is a ball valve device for automatic water inlet, the paramecium breeding room is connected with a paramecium feeding pipe, and the paramecia The feeding pipe is controlled by a time switch through the pump.

The water circulation filtration system includes a diversion tank for recovering the overflow liquid from the siphon cylinder, a water inlet pipe, a water pump and a water storage tank. A filter chamber is provided in the water storage tank to accept the liquid flowing out of the diversion tank, and the water storage tank is connected to the water inlet pipe through a water pump. , the water pump is controlled by the second time switch, a valve is provided on the water inlet pipe, an ultraviolet lamp is set to sterilize the water in the water inlet pipe, and the water storage tank is provided with a water level overflow hole.

The present invention can be used for fully automatic breeding of various fishes that feed on paramecium at the juvenile stage, especially experimental animals such as zebrafish and medaka. The feeding and water changing are fully automated, and the paramecium can be fed regularly every day, thus ensuring the nutritional requirements for the growth of juvenile fish and improving the survival rate and growth rate of juvenile fish.

Description of drawings

Figure 1 is a schematic diagram of the present invention.

Detailed ways

In order to make the above objects, features and advantages of the present invention more comprehensible, specific implementations of the present invention will be described in detail below in conjunction with the accompanying drawings.

The invention consists of three parts: a siphon cylinder (1), a paramecium breeding cylinder (2) and a water circulation filtering system (3). The siphon cylinder (1) is connected to the paramecium breeding cylinder (2) through a feeding pipe (25), and the water circulation filter system (3) recovers the overflowing nutrients from the siphon cylinder (1) through a diversion groove (31). For fish water, the siphon cylinder (1) overflows excess fish water to the water circulation filter system (3) through the inverted "U" (14) pipe. The siphon circular tank (1) is a feeding space for juvenile fish, and the inverted "U" pipe (14) connected to the bottom utilizes the siphon principle to realize automatic control of the water level. The paramecium breeding tank (2) realizes the maintenance and feeding of the paramecium. The water circulation filtering system (3) realizes the recycling of fish farming water.

Paramecium breeding tank

As shown in Figure 1, the paramecium breeding tank (2) includes a flour fermentation room (21) and a paramecium breeding room (22), with an isolation net (23) and a water level control valve (24), and a paramecium feeding chamber (22). Feeding tube (25) and pump (26). The flour fermentation room (21) is a space separated by the isolation net (23) in the paramecium breeding room (22), and the water level control valve (24) is a ball valve device for automatic water intake, and the paramecium breeding room (22) is connected with paramecium feeding pipe (25), and paramecium feeding pipe (25) is controlled by time switch one (27) through pump (26).

The paramecium breeding tank (1) is a food source for juvenile fish. Pure water enters the flour fermentation chamber (21) through the water level control valve (24) and gradually fills the paramecium breeding chamber (22). When the water level reaches the highest level, the float of the water level control valve (24) rises to close the water inlet. A certain amount of flour is added to the flour fermentation chamber (21), and then an appropriate amount of yeast powder is added, stirred evenly and then left to rest for 3 days. After the flour is fermented, an appropriate amount of paramecium mother liquor is dropped into the paramecium breeding chamber (22), and the cultivation is continued for 5 to 7 days. The isolation net (23) has prevented the flour ball from entering the paramecium breeding room, and can keep the water body relatively clean. Paramecia breeding chamber (22) side wall is equipped with paramecia feeding pipe (25), and time switch one (27) controls paramecia liquid to flow out of paramecia breeding tank (2) through pump (26). When the paramecium liquid flows out of the paramecium breeding tank (2), the floating ball of the water level control valve (24) starts to drop, and fresh pure water enters the flour fermentation chamber (21) synchronously, and the fermented flour passes through the isolation filter (23). Flour is injected into the paramecium breeding chamber (22), thereby maintaining the balance of the water level and the continuous supply of the paramecium.

Siphon cylinder

As shown in Figure 1, the siphon cylinder includes a juvenile fish chamber (11) connected to a buffer chamber (13) through a vertical pipe (12), the juvenile fish chamber (11) is the main place for raising juvenile fish, and the buffer chamber (13) is Space to buffer overflow in zebrafish. Sunshade (15) is a light-tight cylinder that wraps buffer chamber (13). The bottom of the buffer chamber (13) is connected to the inverted "U" pipe (14), and the water level of the juvenile fish chamber (11) is automatically controlled by the siphon principle, and the water inlet pipe (36) is close to the inner wall of the juvenile fish chamber (11) to inject the water flow .

During the automatic feeding process, an appropriate amount of paramecium liquid is injected into the juvenile fish room (11) from the feeding tube (25). Since the liquid level is lower than the top of the inverted "U" tube (14), the liquid in the siphon cylinder (1) Does not flow out, allowing juvenile fish ample time to feed. In the automatic water exchange link, the water of the water inlet pipe (36) enters obliquely along the side wall of the juvenile fish chamber (11), so that the water body of the juvenile fish chamber (11) forms a vortex. When the liquid level of the juvenile fish chamber (11) exceeded the top of the inverted "U" pipe (14), redundant fish culture water flowed out automatically, and at this moment the vortex in the juvenile fish chamber (11) was conducive to the discharge of impurities at the bottom of the water. After changing the water, under the effect of siphon, the fish water in the siphon cylinder will continue to flow out until the liquid level in the juvenile fish room (11) is parallel to the end opening of the inverted "U" pipe (14), thereby keeping the juvenile fish. The minimum water level in the fish chamber (11). Buffer chamber (13) is the buffer chamber that prevents zebrafish from flowing out, and the dark environment that light shield (15) provides to buffer chamber (13). The juvenile fish room (11) is connected with the vertical pipe (12) and has a circular filter screen to stop the juvenile fish from flowing out. The juvenile fish chamber (11) is a circular cylinder, and the vortex formed when changing the water can induce the juvenile fish to swim against the current.

Water Circulation Filtration System

The water circulation filtering system (3) includes a diversion tank (31) for recovering the overflow liquid of the siphon cylinder, a water inlet pipe (36), a water pump (34) and a water storage tank (33), and the water storage tank (33) is equipped with a filter chamber ( 32) To accept the liquid flowing out of the diversion tank (31), the water storage tank (33) is connected to the water inlet pipe (36) through the water pump (34), and the water pump (33) is controlled by the timer switch two (38), and the water inlet pipe (36) Valve (37) is arranged on it, ultraviolet lamp (35) is set to disinfect the water in the water inlet pipe (36), and water storage tank (33) is provided with water level overflow hole (39). The diversion groove (31) is a channel for introducing the overflow liquid in multiple siphon cylinders (1) into the water storage tank (33), and the filter chamber (32) is a device for filtering solid impurities in the overflow liquid.

The pisciculture water overflowing from the inverted "U" pipe (14) enters the filter chamber (32) through the diversion groove (31), and the solid impurities in the water body are preliminarily filtered and enter the water storage tank (33). The water pump (34) pumps out the water in the storage tank (33) under the control of the timer switch 2 (38) and sterilizes it through the ultraviolet lamp (35), and finally enters the siphon circle through the water inlet pipe (36) and the valve (37). in cylinder (1). An overflow hole (39) is equipped with on the water storage tank (33) side wall, and when the water level exceeded the maximum liquid level, unnecessary fish farming water was discharged into the sewer through the overflow hole.

Through the combination of the above three parts, automatic breeding and feeding of paramecium, automatic water change and filtration are realized, thereby simplifying the process of raising young fish.

The device of the present invention and its working process will be described in more detail below with reference to the examples.

Example Automatic rearing of juvenile zebrafish

This example takes the rearing of juvenile zebrafish as an example to illustrate the implementation process.

The overall size of the paramecium breeding tank (2) is 30cm*30cm*25cm, the size of the separated flour fermentation chamber is 30cm*8cm*10cm, and the water level control valve (24) is installed on the side wall of the flour fermentation chamber (21) , paramecium feeding pipe (25) opening is 3cm below the liquid level of paramecium fermentation chamber (22). 7 days before the fish eggs are collected, clean the paramecium breeding tank (2) and pour pure water into it. After the water is full, add 5 grams of wheat flour to the flour fermentation chamber (21), add 0.5 grams of yeast powder after stirring, and ferment 3 days. On the 4th day, add 500ml of paramecium mother solution in the paramecium breeding room (22), continue to cultivate for 5 days and use. In the evening of Day 7, pair male and female zebrafish. On the morning of the eighth day, fertilized eggs were collected and cultured in a 16cm glass petri dish with embryo culture medium. Fertilized ovum hatching temperature is 28.5 ℃, removes dead ovum once every day sooner or later, after fish roe is all deshelled, juvenile fish is transferred to the juvenile fish room (11) of siphon cylinder. A circular filter screen with a diameter of 2.0cm (80 orders) is placed between the juvenile fish chamber (11) and the vertical pipe (12). All cavity wall thickness 5mm in this example; The inner diameter of juvenile fish chamber (11) is 18cm, and height is 6cm; Buffer chamber (13) inner diameter is 7cm, and height is 2cm; Vertical tube (12) inner diameter is 1.8cm, and height is 2cm. 2cm; Fall " U " pipe (14) inner diameter is 6mm, and outer diameter is 10mm, and end opening is apart from juvenile fish chamber (11) bottom 3cm, and " U " shape apex is apart from juvenile fish chamber (11) bottom 6cm.

Connect the siphon cylinder (1), the paramecium breeding tank (2) and the water circulation filter system (3). The inverted "U" pipe (14) end opening of the siphon cylinder (1) is placed above the diversion groove (31), the water inlet pipe (36) is placed obliquely along the larvae chamber (11) inwall, and the paramecium feeding pipe (25 ) as above the juvenile chamber (11). Set the paramecium feeding time as 8:00, 10:00, 12:00, 14:00, 16:00 and 18:00 for 3 minutes each, the flow rate is 200ml/min; set the automatic water change time as 8:30 , 10:30, 12:30, 14:30, 16:30 and 18:30 for 5 minutes each, the flow rate is 300ml/min.

Let's take a feeding and water change program as an example.

Start at 8:00 in the morning, timer switch one (27) opens pump (26), and the paramecium liquid in the paramecium breeding chamber (22) enters the juvenile fish chamber (11) along the feeding pipe (25). The three-minute flow of the feeding tube (25) is 600ml, which can make the liquid level of the juvenile fish room (11) rise by about 2cm, and the liquid level cannot submerge the top of the inverted "U" tube (14), and the paramecium liquid does not flow out. Siphon round tank (1), the zebrafish have enough time to eat. Simultaneously, liquid level descends in the paramecium breeding room (22), the ball valve of the water level control valve ball (24) in the flour fermentation room (21) also reduces thereupon, begins to inject pure water, thereby maintains the paramecium breeding cylinder (2) The stability of the inner liquid level. And, the newly injected pure water drives the fermented product of flour to pass through the filter screen (23) thereby supplementing the food of the paramecium, so that the paramecium can continue to reproduce.

At 8:30 in the morning, the timer switch two (38) starts the water pump (34) of the water circulation filtration system (3), and the fish water in the water storage tank (33) is disinfected by ultraviolet rays, and the water is discharged along the side wall of the juvenile fish room (11). Inject at an angle. Regulate the valve (37) of water inlet pipe, make liquid form a weak vortex in the juvenile fish room (11). When fish water was not crossed " U " pipe (14) top, unnecessary fish water flowed out from the end of " U " pipe, thus started the draining process of siphon cylinder. Juvenile zebrafish generally swim against the vortex, and most juveniles will not be swept away by this slow current. An 80 order circular filter screen is arranged between the juvenile fish room (11) and the vertical pipe (12), which can stop the escape of juvenile fish. There is a buffer chamber (13) at the bottom of the juvenile fish chamber (11), which utilizes the characteristics of phototaxis and countercurrent flow of zebrafish, and is a buffer space for reducing the escape of zebrafish.

After the fish farming water flows out from the inverted "U" pipe (14), it enters the diversion tank (31) of the water circulation filter system (3), then through the filter chamber (32) and finally gathers in the water storage tank (33), from form a water cycle. At the end of the water intake, the inverted "U" tube (14) will continue to remove the fish culture water in the siphon cylinder (1) until the water surface of the juvenile fish room (11) is flush with the opening at the end of the inverted "U" tube (14) , fish farming water no longer flows out, thereby completing an automatic water change.

In this implementation case, there were 180 juvenile zebrafish on the 3rd day after fertilization and 151 survived on the 13th day after fertilization in the juvenile fish room (11) for the first time. In the control group, which adopts artificial water change and feeding, 240 animals were raised initially, and 199 animals survived on the 13th day after fertilization. Statistics on the body length of the juvenile zebrafish raised in the control group and the device showed that the body length of the juvenile fish raised in the device was significantly better than that of the control group (Table 1). This is because the feeding and water changing of the device are fully automated, and paramecium can be fed regularly six times a day, which fully guarantees the nutritional requirements for the growth of juvenile fish and improves the survival rate and growth speed of juvenile fish.

As mentioned above, the device can realize the automatic feeding of the young fish after hatching, thereby greatly simplifying the related operations and improving the experimental efficiency.

Table 1

the test group control group significant survival rate 83.9 (151/180) 82.9 (199/240) the Body length 5.136 ±0.03014 4.125 ±0.01787 P<0.0001

Claims (10)

1. the full-automatic raising device of juvenile fish, comprise: siphon circle cylinder (1), paramecium breeding cylinder (2), water circulation filtering system (3), it is characterized in that: described siphon circle cylinder (1) and described paramecium breed cylinder (2) and manage (25) by throwing something and feeding and be connected, described water circulation filtering system (3) reclaims siphon circle cylinder (1) water of breeding fish that overflows by guiding gutter (31), and described siphon circle cylinder (1) overflows unnecessary water of breeding fish to water circulation filtering system (3) by falling " U " (14) pipe.

2. the full-automatic raising device of juvenile fish according to claim 1, it is characterized in that: described siphon circle cylinder (1) comprises juvenile fish room (11) and surge chamber (13), juvenile fish room (11) is connected with surge chamber (13) and by vertical tube (12), surge chamber (13) be blocked cover (15) around, surge chamber (13) bottom with fall " U " manage (14) and be connected.

3. the full-automatic raising device of juvenile fish according to claim 2, is characterized in that, light shield (15) is the light tight drum around surge chamber (13).

4. the full-automatic raising device of juvenile fish according to claim 2, is characterized in that, juvenile fish room (11) and vertical tube (12) connecting place have a circular filter screen.

5. the full-automatic raising device of juvenile fish according to claim 1, it is characterized in that, described paramecium breeding cylinder (2) is divided into flour fermenting cellar (21) and paramecium propagation house (22) by separation net (23), and is provided with altitude valve (24) and paramecium and throws something and feeds pipe (25).

6. the full-automatic raising device of juvenile fish according to claim 5, is characterized in that, paramecium pipe (25) of throwing something and feeding is connected with pump (26), and controls by time switch one (27).

7. the full-automatic raising device of juvenile fish according to claim 1, it is characterized in that, described water circulation filtering system (3) comprises the guiding gutter (31) reclaiming siphon circle cylinder overflowing liquid, water inlet pipe (36), ultraviolet lamp tube (35), water pump (34) and water storage cylinder (33), the liquid that filter chamber (32) flows out to accept guiding gutter (31) is established in water storage cylinder (33), water storage cylinder (33) sidewall top is provided with a water level overfolw hole (39), water storage cylinder (33) connects water inlet pipe (36) by water pump (34), the current of water inlet pipe are through ultraviolet lamp tube (35).

8. the full-automatic raising device of juvenile fish according to claim 7, is characterized in that, water pump (34) is controlled by time switch two (38), and water inlet pipe (36) is provided with and valve (37).

9. the full-automatic raising device of juvenile fish according to claim 7, is characterized in that, described guiding gutter (31) is that overflowing liquid in multiple siphon circle cylinder (1) is introduced the passage of water storage cylinder (33).

10. the full-automatic raising device of juvenile fish according to claim 1, is characterized in that, multiple siphon justifies cylinder (1) and column distribution, forms a full-automatic feeding system of juvenile fish.