CN102352340B - Pallium cell fragment treating fluid used in Pinctada martensii pearl breeding - Google Patents
Pallium cell fragment treating fluid used in Pinctada martensii pearl breeding Download PDFInfo
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- CN102352340B CN102352340B CN 201110275368 CN201110275368A CN102352340B CN 102352340 B CN102352340 B CN 102352340B CN 201110275368 CN201110275368 CN 201110275368 CN 201110275368 A CN201110275368 A CN 201110275368A CN 102352340 B CN102352340 B CN 102352340B
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- pearl
- treating fluid
- small pieces
- treatment solution
- mantle cell
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Abstract
The invention relates to a pallium cell tablet treating fluid used in Pinctada martensii pearl breeding. The treating fluid comprises 20-30g of calcium propionate, 0.02-0.05g of doxycyline and 1000mL of distilled water. The preparation method comprises the following steps: successively and completely dissolving 20-30g of calcium propionate and 0.02-0.05g of doxycyline in distilled water and evenly stirring so as to obtain the pallium cell tablet treating fluid used in Pinctada martensii pearl breeding. By adopting the treating fluid to treat the pallium cell tablet, the surgery bacteria infection can be effectively inhibited, the wound healing is promoted, and the survival rate, nucleus remaining rate and pearl quality of pearl-breeding Pinctada martensii are effectively improved.
Description
Technical field
The present invention relates to a kind for the treatment of solution of growing cultured pearls and using, be specifically related to the mantle cell small pieces treatment solution that a kind of pteria martensii uses in growing cultured pearls, belong to pearl culture treatment solution technical field.
Background technology
Cultivate the process of pearl (nucleated pearl and no nucleus pearl) and all need in the visceral mass of Margarita, implant mantle cell small pieces, the epithelial cell of transplanted mantle cell small pieces forms pearl sac through migration, propagation, parcel (pearl nuclear), secretes nacre then and forms pearl.The pearl cultivating process comprises links such as the pre-treatment of pearly shellfish art, the processing of cell small pieces, the recuperation of pearly shellfish postoperative.And the suitable processing of mantle cell small pieces has important effect (Xie Yukan, etc., 1995) to improving core-remaining rate, Margarita surviving rate and pearl quality.
At present disclosed treatment solution or the composition of Precerving liquid for the treatment of pteria martensii mantle cell small pieces is various, mainly contains duomycin; Plant hormones regulators,gibberellins; polyvinylpyrrolidone; taurine; vitamins C; Prednisone acetate; oligochitosan; the bright promise R2 of fluorescein Ilyushin; merbromin; penicillin; Regular Insulin; Yelkin TTS; cobalt chloride; Manganous chloride tetrahydrate; magnetized water; calglucon; shellfish (freshwater mussel) tissue leach liquor or extracting solution; Sodium ATP (ATP); amino acid; Viola crystallina; Norxin; gentamicin sulphate; sodium alginate; Radix Glycyrrhizae; trypterygine; NaCl; KCl; MgCl
2, CaCl
2, wait (Wang Meifang, etc., 2010).Chinese patent application " small pieces that a kind of pteria martensii is grown cultured pearls and pearl nuclear matching treatment liquid (application number 201010275108.3) " discloses the small pieces that a kind of pteria martensii grows cultured pearls and has examined matching treatment liquid with pearl: " this treatment solution with raw material weighing such as duomycin, polyvinylpyrrolidone, taurine and vitamins Cs after, the normal saline solution that above-mentioned raw materials is dissolved in weight content 0.7-1% sodium alginate obtains ".Chinese patent application " a kind of novel maintenance liquid for mantle pieces of pinctada martensii and preparation method thereof (application number 200910194336.5) " discloses a kind of novel maintenance liquid for mantle pieces of pinctada martensii and preparation method thereof: " by NaCl, KCl, MgCl
2, CaCl
2Form weighing NaCl, KCl, MgCl with Prednisone acetate
2, CaCl
2And Prednisone acetate, be dissolved in distilled water successively, adopt NaHCO
3Regulate the pH value to 7.8-8.0 ".Chinese patent application " a kind of method (application number 200810148954.1) of handling mantle piece raising quality of sea water pearl " discloses a kind of method that mantle piece improves quality of sea water pearl of handling: " select the oligochitosan of separation and purification, be mixed with the solution that concentration is 0.5-3% with the degerming seawater; Cut the peripheral edge portion that has the mantle than the mantle non-pigment particle of strong splitting ability from the mantle piece shellfish, soak mantle piece after 5-10 minute, perhaps similar concentration solution is smeared about 10 minutes of back placement, is cut into the mantle piece of long and the wide 4.5-5.5 of being millimeter again; Mantle piece and the pearl nuclear of oligochitosan solution-treated are implanted in the bag of growing cultured pearls of pinctada together ".Disclosed medicine or the effect of method for the treatment of pteria martensii mantle cell small pieces is not ideal enough for these, adopts these method pteria martensii surviving rates, core-remaining rate relatively low, and pearl quality is not fine.
Summary of the invention
The purpose of this invention is to provide a kind of new and effective pteria martensii mantle cell small pieces treatment solution that can obviously improve pteria martensii surviving rate, core-remaining rate and pearl quality.
In order to address the above problem, the technical solution adopted in the present invention is: the mantle cell small pieces treatment solution that uses during a kind of pteria martensii is grown cultured pearls, formed by following prescription:
Calcium propionate 20 ~ 30 grams
Doxycycline 0.02～0.05 gram
1000 milliliters of distilled water
Doxycycline claims Vibravenos, doxycycline again;
The preparation method of above-mentioned " the mantle cell small pieces treatment solution that a kind of pteria martensii uses in growing cultured pearls " is (each component is measured by above-mentioned prescription): be dissolved in the distilled water fully doxycycline, calcium propionate successively, stir, just obtain " the mantle cell small pieces treatment solution that a kind of pteria martensii uses in growing cultured pearls ".The present invention serves as that the main treatment solution that is mixed with is handled pteria martensii mantle cell small pieces with doxycycline, calcium propionate, is used for cultivating the seawater nucleated pearl and obtains effect preferably.
Replace the polyvinylpyrrolidone sea water solution described in Guangdong Province's provincial standard " DB44/T 330-2006 pteria martensii cultural technique standard-slotting nuclear technique working specification " with " the mantle cell small pieces treatment solution that a kind of pteria martensii uses in growing cultured pearls " of the present invention, soak pteria martensii mantle cell small pieces 3min, other operation is undertaken by the aforementioned operation rules fully, and by Guangdong Province's provincial standard " DB44/T 328-2006 pteria martensii cultural technique standard-pearly shellfish cultural technique " cultivation pearl, can effectively suppress the infectation of bacteria of performing the operation, promote wound healing, the pearly shellfish surviving rate, core-remaining rate and excellent pearl rate improve 10% respectively, about 20 ~ 30% and 20 ~ 30%.
The present invention with respect to the beneficial effect of prior art is: adopt this treatment solution can effectively suppress the infectation of bacteria of performing the operation, promote wound healing, effectively improve pearly shellfish surviving rate, core-remaining rate and pearl quality.
Embodiment
Be described in further details below by the present invention of embodiment, these embodiment only are used for illustrating the present invention, do not limit the scope of the invention.
Embodiment 1
0.02 gram doxycycline, 20 gram calcium propionates are dissolved in 1000 ml distilled waters successively, stir, just obtain " the mantle cell small pieces treatment solution that a kind of pteria martensii uses in growing cultured pearls ".
Embodiment 2
0.03 gram doxycycline, 25 gram calcium propionates are dissolved in 1000 ml distilled waters successively, stir, just obtain " the mantle cell small pieces treatment solution that a kind of pteria martensii uses in growing cultured pearls ".
Embodiment 3
0.04 gram doxycycline, 30 gram calcium propionates are dissolved in 1000 ml distilled waters successively, stir, just obtain " the mantle cell small pieces treatment solution that a kind of pteria martensii uses in growing cultured pearls ".
Embodiment 4
0.05 gram doxycycline, 22 gram calcium propionates are dissolved in 1000 ml distilled waters successively, stir, just obtain " the mantle cell small pieces treatment solution that a kind of pteria martensii uses in growing cultured pearls ".
Embodiment 5
0.05 gram doxycycline, 28 gram calcium propionates are dissolved in 1000 ml distilled waters successively, stir, just obtain " the mantle cell small pieces treatment solution that a kind of pteria martensii uses in growing cultured pearls ".
Experimental data is as follows:
Time: 2010.9.13～2011.06.16 (9 months 3 days) is convenient statistics core-remaining rate, and every shellfish only inserts a pearl nuclear
The test-results of growing cultured pearls: surviving rate, core-remaining rate and excellent pearl rate improve 11%, 28% and 33.3% respectively.
Claims (2)
1. the mantle cell small pieces treatment solution that uses during a pteria martensii is grown cultured pearls, it is characterized in that: this treatment solution is made up of following material: calcium propionate: 20 ~ 30 grams, doxycycline: 0.02～0.05 gram, distilled water: 1000 milliliters.
2. the preparation method of the mantle cell small pieces treatment solution that uses during a pteria martensii is grown cultured pearls, it is characterized in that being dissolved in 20 ~ 30 gram calcium propionates and 0.02～0.05 gram doxycycline in 1000 ml distilled waters successively fully, stir, just obtain the mantle cell small pieces treatment solution that a kind of pteria martensii uses in growing cultured pearls.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110275368 CN102352340B (en) | 2011-09-16 | 2011-09-16 | Pallium cell fragment treating fluid used in Pinctada martensii pearl breeding |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110275368 CN102352340B (en) | 2011-09-16 | 2011-09-16 | Pallium cell fragment treating fluid used in Pinctada martensii pearl breeding |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102352340A CN102352340A (en) | 2012-02-15 |
| CN102352340B true CN102352340B (en) | 2013-07-17 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110275368 Expired - Fee Related CN102352340B (en) | 2011-09-16 | 2011-09-16 | Pallium cell fragment treating fluid used in Pinctada martensii pearl breeding |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108617561B (en) * | 2018-04-10 | 2020-03-27 | 广东海洋大学 | Method for improving nucleus retention rate and high-quality pearl rate of pinctada martensii after being planted with nucleus |
| CN113207771A (en) * | 2021-05-08 | 2021-08-06 | 北海市源龙珍珠有限公司 | Multiple amino acid calcium culture solution for culturing pearls |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101356901B (en) * | 2008-09-12 | 2011-01-26 | 海南大学 | Method for pearl core treatment by medicament |
| CN101724598B (en) * | 2009-11-24 | 2012-05-30 | 广东海洋大学 | Novel maintenance liquid for mantle pieces of pinctada martensii |
| CN101991588B (en) * | 2010-09-08 | 2012-08-08 | 广西壮族自治区水产研究所 | Flake and pearl nucleus matching treating fluid for culturing Pinctada martensii pearl |
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2011
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