CN108849658A - A method of improving pteria martensii eyebot larvae metamorphosis adhesive rate - Google Patents

A method of improving pteria martensii eyebot larvae metamorphosis adhesive rate Download PDF

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Publication number
CN108849658A
CN108849658A CN201810592237.1A CN201810592237A CN108849658A CN 108849658 A CN108849658 A CN 108849658A CN 201810592237 A CN201810592237 A CN 201810592237A CN 108849658 A CN108849658 A CN 108849658A
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pond
collector
eyebot larvae
eyebot
pteria martensii
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CN108849658B (en
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李俊辉
梁飞龙
邓岳文
符韶
王庆恒
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Guangdong Zunding Pearl Co ltd
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Guangdong Ocean University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • A01K61/50Culture of aquatic animals of shellfish
    • A01K61/54Culture of aquatic animals of shellfish of bivalves, e.g. oysters or mussels
    • A01K61/56Culture of aquatic animals of shellfish of bivalves, e.g. oysters or mussels for pearl production
    • A01K61/57Pearl seeds
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • A01K61/50Culture of aquatic animals of shellfish
    • A01K61/54Culture of aquatic animals of shellfish of bivalves, e.g. oysters or mussels
    • A01K61/56Culture of aquatic animals of shellfish of bivalves, e.g. oysters or mussels for pearl production
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Farming Of Fish And Shellfish (AREA)

Abstract

The invention discloses a kind of methods for improving pteria martensii eyebot larvae metamorphosis adhesive rate, belong to technical field of aquaculture.This method basic step includes:Pteria martensii eyebot larvae attachment pond prepares, eyebot larvae is collected, drug is fed, seedling management;In pteria martensii seedling raising process, collect the eyebot larvae of not yet abnormal attachment in nursery pond, it moves on in the nursery pond for overworked collector and cultivates, improve ingesting and digesting for eyebot larvae using antibiotic and vitamin, improve the abnormal adhesive rate of pteria martensii eyebot larvae.The nursery of pteria martensii large-scale artificial is carried out using this method, larval metamorphosis adhesive rate is high, and Individual Size is uniform, can be improved the nursery yield and economic benefit of pteria martensii;The present invention is suitable for the nursery of pteria martensii large-scale artificial, is easy to promote and apply, Social benefit and economic benefit is significant.

Description

A method of improving pteria martensii eyebot larvae metamorphosis adhesive rate
Technical field
The present invention relates to a kind of methods for improving pteria martensii eyebot larvae metamorphosis adhesive rate, belong to aquaculture technology Field is suitable for the nursery of pteria martensii large-scale artificial and produces.
Background technique
Pteria martensii(Pinctada martensii)Also known as pinctada fucata is the main of China breeding seawater pearl Shellfish kind is claimed " Nan Zhu " with the pearl that pteria martensii cultivates, and the pearl of production accounts for 90% or more of sea water pearls yield." south Pearl " cultivation is the specialty industries of China's agricultural and the pillar industry of Guangdong Province Lezhou Peninsula coastal area, when peak to cultivate face Product surpasses 5.38 ten thousand mu, and 4.5 ten thousand people are engaged in pearl sector, 20 tons of pearl annual output or so, accounts for 70% or more of national yield, is formed Seedling fostering, female shellfish cultivation, plant core grow cultured pearls, one huge industrial chain of pearl processing and sale, the entire industrial chain gross output value Up to billions of members, positive contribution is made that for shaking off poverty and setting out on the road to prosperity for Lezhou Peninsula coastal area peasant.
Currently, it is that breed variety is degenerated, breeding environment deteriorates and cultural technique is old that " Nan Zhu ", which produces existing main problem, It is old, lead to that the pearly shellfish death rate is high, core-remaining rate is low, pearl quality is poor, " Nan Zhu " annual output only has 2-3 tons, and culture efficiency is substantially Degree glides, and raiser changes one's profession or closes down one after another.For the predicament that south-pearl industry faces, Guangdong provincial people's government implements to revitalize south There is slow recovery in pearl industry plan, pearl sector, but are faced with the problem of high-quality cultivation seedling insufficient supply.Geneva pearl oyster During shellfish seedling fostering, nursery enterprise and nursery carry out artificial breeding by the way of not changing water and repeatedly launching collector, Collector usually takes ability complete metamorphosis attachment in 6-10 days after launching, dead in nursery pond after being launched 5-7 days due to collector Algae and the diatom newly grown, larva secretion and water body in bacterium etc. be adhered to and make to adhere on the lamina affixad of collector Plate, which becomes, to be slided, and causes not yet abnormal larva cannot the abnormal attachment on lamina affixad;Secondly, even if the abnormal larva individual newly adhered to It is small, cause out pond seedling size it is uneven;Third, if the attachment of eyebot larvae long-time unchangeable metamorphosis will natural death or logical It crosses and changes water and exhaust, cause eyebot larvae utilization rate low.In order to improve nursery benefit, enough high quality seedlings are provided for raiser, are mentioned The abnormal adhesive rate of high eyebot larvae seems particularly significant.
Summary of the invention
The present invention produces the low situation of existing eyebot larvae utilization rate for pteria martensii nursery, and providing one kind can The method for improving pteria martensii eyebot larvae metamorphosis adhesive rate, economic benefit is significant with social benefit, can be used in geneva pearl Female shellfish large-scale artificial nursery production.
Technical scheme is as follows:
A method of pteria martensii eyebot larvae metamorphosis adhesive rate being improved, basic step is as follows:
(1)The preparation in indoor raising of seedling pond:Selection has into pumping equipment, floor space 20-30m2, the indoor pond of high 1.5m, Pond shape be it is rectangular, convenient for setting up rope or bamboo above pond for hanging collector, every 4-6 quadrature sets scattered One, gas stone.Collector is black and red plastic sheet, and every string collector length 1.2-1.3m, collector piece spacing is 10- 12cm.Pond and collector use preceding cleaned and disinfection treatment, pond chlorine-containing disinfectant or disinfecting solution of potassium permanganate Processing is handled with disinfecting solution of potassium permanganate after collector weak hydrochloric acid soaking flushing, hydrochloric acid can quickly dissolve be attached to it is old Bur on algae and new collector on collector.
(2)The collection of eyebot larvae:Collector has been launched 5-7 days still in water body with the double-deck mesh bag collection using siphonage In the eyebot larvae that swims, inner layer net pocket diameter is greater than outer layer, the eyebot larvae that internal layer mesh bag is collected(Individual is larger)With it is outer The larva that layer is collected(Individual is smaller)It moves on in different ponds and cultivates respectively.Internal layer mesh bag is 120 mesh, 200 mesh of outer layer, in dark The middle water body irradiated after collecting larva with flashlight light has seen whether that leakage seedling occurs, and to stop collecting at once if there is leakage seedling And replace and adjust mesh bag specification.It is collected simultaneously the eyebot larvae of multiple nursery ponds, makes a nursery pond in a short time Eyebot larvae quantity moves back another pond up to 0.5-0.8/ml.
(3)Drug is fed:Larva will receive damage during collection, use the furazolidone or hydrochloric acid of 0.5mg/L Ciprofloxacin is used continuously 3 days, to prevent injured infection, improves the functions of intestines and stomach of larva.The dimension for feeding 0.1-0.2mg/L is raw Plain B1 and B2, uses every other day.Vitamin B1 and B2 have the function of improving biological metabolism and digestive conditions.Antibiotic and vitamin Be ground into powder the full pool spilling head after 300 mesh net filtrations.
(4)Seedling management:The throw-in density of larva is 0.5-0.8/ml, and the depth of water is adjusted to 1.4 after launching eyebot larvae Rice, makes collector not expose the surface, collector spacing 20-25cm.It is conventionally inflated, changes water and bait throwing in.
(5)The environmental condition of pteria martensii artificial breeding is:23-29 DEG C of ocean temperature, relative density 1.018- 1.023g/cm3
The present invention having the beneficial effect that compared with the existing technology:
(1)Currently, the use of pteria martensii artificial breeding does not change water and repeatedly launches lamina affixad(Collector)Mode, 30% or more Larva occurs that lamina affixad is laid in nursery bottom of pond portion after eyespot, gets after throwing plate 3-5 days and hangs in nursery pond or move on to Other pond hanging cultures.After former nursery pond overworks lamina affixad, slow larva is developed also in floating state, is glued on lamina affixad at this time The diatom of the mycoderm, growth attachment that are formed with dead frustule, bacterium causes the eyebot larvae to swim to be difficult to abnormal attached ?.
(2)The eyebot larvae collection of not yet metamorphosis attachment moves on in new nursery pond after the present invention will launch lamina affixad 5-7 days, Allow larva is abnormal in completely new environment to adhere to.Antibiotic is launched for three days on end, can prevent injured infection during larvae collection, The digestion for improving larva simultaneously is horizontal;Vitamin is fed every other day, can improve the metabolism and digestive conditions of larva.It therefore, can be with Improve the distortion ratio of eyebot larvae.
(3)Larva collects specification relatively by different mesh the screen to filtrates, and pond seedling individual is uniform out, by raiser It welcomes.
Operation of the present invention is convenient, can be improved the utilization rate of eyebot larvae, to improve nursery benefit.It is easy raw in seedling It produces and is promoted in enterprise, be suitable for pteria martensii artificial breeding and produce, economical, societal benefits are significant.
Specific embodiment
The present invention is described in further details below by embodiment, these embodiments are only used to illustrate the present invention, and It does not limit the scope of the invention.
Embodiment 1
Pteria martensii seed rearing is carried out in Zhanjiang Feng Lian aquatic products Co., Ltd base, using the method June in April, 2015- The distortion ratio of the eyespot young improves 15.7%, and specific implementation measure is as follows:
(1)The preparation in indoor raising of seedling pond:Selection has into pumping equipment, floor space 20m2, the indoor fountain square of high 1.5m, Rope is set up above pond for hanging collector, distance is greater than the lamina affixad 40cm of collector, every 4 squares of faces between rope Product sets gas exhausting stone one.Collector is black plastic piece, and every string length 1.2m, the attachment plate spacing of collector is 10cm.Pond It is disinfected using the preceding Eusol with available chlorine content 20mg/L, collector is used with 3% salt acid soak 6h of content or more Seawater flushing is filtered completely to be handled with 30mg/L disinfecting solution of potassium permanganate again;
(2)The collection of eyebot larvae:Collector is launched 5 days with the double-deck mesh bag collection using siphonage still to swim in water body Eyebot larvae, inner layer net pocket diameter(120 mesh)Greater than outer layer(200 mesh), eyebot larvae that internal layer mesh bag is collected(Individual compared with Greatly)The larva collected with outer layer(Individual is smaller)It moves on in different ponds and cultivates respectively, cultivate 0.5/ml of density.In the dark It has seen whether that leakage seedling occurs with the water body that flashlight light irradiates after collecting larva, to have stopped collecting simultaneously at once if there is leakage seedling Replacement and adjustment mesh bag specification;
(3)Drug is fed:Larva will receive damage during collection, using the furazolidone of 0.5mg/L, be used continuously 3 days, To prevent injured infection, improve the functions of intestines and stomach of larva.The vitamin B1 and B2 for feeding 0.1mg/L, use every other day.Vitamin B1 Have the function of improving biological metabolism and digestive conditions with B2.Antibiotic and vitamin are ground into powder through 300 mesh screen mistakes Full pool spilling head after filter;
(4)Seedling management:The throw-in density of larva is 0.5/ml, and the depth of water is adjusted to 1.4 meters after launching eyebot larvae, makes to collect seedling Device does not expose the surface, collector spacing 20cm.It conventionally inflates, change water and bait throwing in;
(5)The environmental condition of pteria martensii artificial breeding is:23-29 DEG C of ocean temperature, relative density 1.018-1.023g/ cm3
Embodiment 2
Pteria martensii seed rearing is carried out in Zhanjiang Feng Lian aquatic products Co., Ltd base, using the method June in March, 2016- The distortion ratio of the eyespot young improves 18.2%, and specific implementation measure is as follows:
(1)The preparation in indoor raising of seedling pond:Selection has into pumping equipment, floor space 30m2, the indoor fountain square of high 1.5m, Bamboo is set up above pond for hanging collector, every 6 quadrature sets gas exhausting stone one.Collector is red plastic piece, Every string collector length 1.3m, the attachment plate spacing for forming collector is 12cm.Pond uses the preceding potassium permanganate with 40mg/L Solution disinfection processing, the salt acid soak 6h or more of collector concentration 3% use the Gao Meng of 40mg/L with filtering sea repeated flushing again Sour potassium solution disinfection treatment;
(2)The collection of eyebot larvae:Collector is launched 7 days with the double-deck mesh bag collection using siphonage still to swim in water body Eyebot larvae, internal layer mesh bag(120 mesh)Aperture is greater than outer layer(200 mesh), eyebot larvae that internal layer mesh bag is collected(Individual compared with Greatly)The larva collected with outer layer(Individual is smaller)It moves on in different ponds and cultivates respectively, 0.8/ml of larvae cultivation density.Black It has seen whether that leakage seedling occurs with the water body that flashlight light irradiates after collecting larva in the dark, to have stopped receiving at once if there is leakage seedling Collect and replaces and adjust mesh bag specification;
(3)Drug is fed:Larva will receive damage during collection, use the Ciprofloxacin Hydrochloride of 0.5mg/L, continuous use 3 days, to prevent injured infection, improve the functions of intestines and stomach of larva.The vitamin B1 and B2 for feeding 0.2mg/L, use every other day, dimension life Plain B1 and B2 has the function of improving biological metabolism and digestive conditions.Antibiotic and vitamin are ground into powder through 300 meshes Full pool spilling head after net filtration;
(4)Seedling management:The throw-in density of larva is 0.8/ml, and the depth of water is adjusted to 1.4 meters after launching eyebot larvae, makes to collect seedling Device does not expose the surface, collector spacing 25cm.It is conventionally inflated, changes water and bait throwing in;
(5)The environmental condition of pteria martensii artificial breeding is:23-29 DEG C of ocean temperature, relative density 1.018-1.023g/ cm3
Embodiment 3
Pteria martensii seed rearing is carried out in Zhanjiang Feng Lian aquatic products Co., Ltd base, using the method June in April, 2017- The distortion ratio of the eyespot young improves 18.7%, and specific implementation measure is as follows:
(1)The preparation in indoor raising of seedling pond:Selection has into pumping equipment, floor space 30m2, the indoor fountain square of high 1.5m, Bamboo is set up above pond for hanging collector, every 5 quadrature sets gas exhausting stone one.Collector is red plastic piece, Every string collector length 1.3m, the attachment plate spacing for forming collector is 12cm.Pond uses the preceding potassium permanganate with 40mg/L Solution disinfection processing, the salt acid soak 6h or more of collector concentration 3% use the Gao Meng of 40mg/L with filtering sea repeated flushing again Sour potassium solution disinfection treatment;
(2)The collection of eyebot larvae:Collector is launched 7 days with the double-deck mesh bag collection using siphonage still to swim in water body Eyebot larvae, internal layer mesh bag(120 mesh)Aperture is greater than outer layer(200 mesh), eyebot larvae that internal layer mesh bag is collected(Individual compared with Greatly)The larva collected with outer layer(Individual is smaller)It moves on in different ponds and cultivates respectively, 0.7/ml of larvae cultivation density.Black It has seen whether that leakage seedling occurs with the water body that flashlight light irradiates after collecting larva in the dark, to have stopped receiving at once if there is leakage seedling Collect and replaces and adjust mesh bag specification;
(3)Drug is fed:Larva will receive damage during collection, use the Ciprofloxacin Hydrochloride of 0.5mg/L, continuous use 3 days, to prevent injured infection, improve the functions of intestines and stomach of larva.The vitamin B1 and B2 for feeding 0.2mg/L, use every other day, dimension life Plain B1 and B2 has the function of improving biological metabolism and digestive conditions.Antibiotic and vitamin are ground into powder through 300 meshes Full pool spilling head after net filtration;
(4)Seedling management:The throw-in density of larva is 0.7/ml, and the depth of water is adjusted to 1.4 meters after launching eyebot larvae, makes to collect seedling Device does not expose the surface, collector spacing 25cm.It is conventionally inflated, changes water and bait throwing in;
(5)The environmental condition of pteria martensii artificial breeding is:23-29 DEG C of ocean temperature, relative density 1.018-1.023g/ cm3

Claims (4)

1. a kind of method for improving pteria martensii eyebot larvae metamorphosis adhesive rate, it is characterised in that:Include the following steps:
(1)The preparation in indoor raising of seedling pond:Selection has into the indoor pond of pumping equipment, high 1.5m, and every 4-6 cubes sets air-dispersing One, stone, pond and collector use preceding sterile-processed;
(2)The collection of eyebot larvae:The eyebot larvae of not yet abnormal attachment in nursery pond is collected with the double-deck mesh bag using siphonage, Inner layer net pocket diameter is greater than outer layer, and the larva that eyebot larvae and outer layer that internal layer mesh bag is collected are collected is moved on to step respectively(1) It is cultivated in the different ponds of preparation;Meanwhile having seen whether that leakage seedling occurs using the water body that flashlight light irradiates after collecting larva, Stop collecting if there is leakage seedling and replaces and adjust mesh bag specification;
(3)Drug is fed:Antibiotic is launched in nursery pond and vitamin, antibiotic are continuously launched 3 days, and vitamin is thrown every other day It puts, antibiotic and vitamin are ground into powder and filter full pool spilling head, and the depth of water keeps 1.2-1.3m;
(4)Seedling management:The throw-in density of larva is 0.5-0.8/ml, and the depth of water is adjusted to 1.4 meters after launching eyebot larvae, is made Collector does not expose the surface, and collector spacing 20-25cm is conventionally inflated, changes water and bait throwing in;
(5)Larval rearing water environmental index:23-29 DEG C of ocean temperature, relative density 1.018-1.023g/cm3
2. improving the method for pteria martensii eyebot larvae metamorphosis adhesive rate according to claim 1, it is characterised in that:It is described Indoor pond is:With into the indoor pond of pumping equipment, high 1.5m, floor space 20-30m2, every 4-6 cubes of water body set air-dispersing One, stone, pond and collector use preceding sterile-processed;Collector is black and red plastic sheet, every string collector length 1.2-1.3m, the attachment plate spacing for forming collector is 10-12cm, pond chlorine-containing disinfectant or disinfecting solution of potassium permanganate It handles, is handled after collector weak hydrochloric acid soaking flushing with disinfecting solution of potassium permanganate.
3. a kind of method for improving pteria martensii eyebot larvae metamorphosis adhesive rate according to claim 1, it is characterised in that: Step(2)Eyebot larvae is to have launched the collector 5-7 days eyebot larvaes still to swim in water body, using siphonage bilayer Mesh bag is collected, and internal layer mesh bag is 120 mesh, and 200 mesh of outer layer is collected simultaneously the eyebot larvae of multiple nursery ponds, and quantity reaches 0.5-0.8 Another pond is then moved on to after a/ml.
4. improving the method for pteria martensii eyebot larvae metamorphosis adhesive rate according to claim 1, it is characterised in that:Step (3)The antibiotic is furazolidone or Ciprofloxacin Hydrochloride, and continuous to launch 3 days, vitamin is B1 and B2, is thrown every other day Hello, required drug is ground into powder the full pool spilling head after 300 mesh net filtrations.
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