CN113475439B - Method for improving seedling yield of pearl oyster - Google Patents
Method for improving seedling yield of pearl oyster Download PDFInfo
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- CN113475439B CN113475439B CN202110979932.5A CN202110979932A CN113475439B CN 113475439 B CN113475439 B CN 113475439B CN 202110979932 A CN202110979932 A CN 202110979932A CN 113475439 B CN113475439 B CN 113475439B
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- pond
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- glutamic acid
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- pearl oyster
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/54—Culture of aquatic animals of shellfish of bivalves, e.g. oysters or mussels
- A01K61/56—Culture of aquatic animals of shellfish of bivalves, e.g. oysters or mussels for pearl production
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention discloses a method for improving the yield of pearl oyster seedlings, which comprises the following steps: (1) Cleaning the attachment medium, soaking in 0.8-1.5% potassium permanganate solution for 12-24h, cleaning with fresh water for 3-4 times, washing with seawater, spreading on the bottom of culture pond, and adding seawater; (2) Collecting eyespot larvae which are not metamorphously attached in the nursery pond, and transferring the collected larvae to the water pond in the step (1) for cultivation; (3) Adding glutamic acid into the pond with the non-metamorphosis attached eyespot larvae in the step (2), wherein the concentration of the glutamic acid is 1 x 10 < -6 > to 5 x 10 < -4 > M, and the treatment time is 2 to 48 hours after the glutamic acid is added; (4) Aerating, changing water and feeding according to a conventional method, and performing shellfish spatfall operation. The method can obviously improve the metamorphosis rate of the eyespot larvae of the pearl oyster through the glutamic acid induction effect, thereby improving the seedling raising level of the pearl oyster.
Description
Technical Field
The invention belongs to the technical field of aquaculture, and particularly relates to a method for improving the seedling yield of pearl oyster.
Background
Pearl shells belong to bivalves, and mussels and scallops, etc. are the species living on rocks, coral reefs, gravels or other shells by foot filaments. Pearl oyster is a warm sea product, and is very popular in the Fujian of China, especially in the coast of Guangdong. There are many kinds of pearl shells, including pearl shell, pearl oyster, pinctada martensii, and Pinctada penguin. Wherein, the pearl cultured by the Pinctada martensii is called 'Nanzhu' with the most common Pinctada martensii, and the pearl produced by the method accounts for more than 90 percent of the yield of seawater pearls. The cultivation of Nanzhu is a characteristic industry of China agriculture and is also a supporting column industry in the coastal region of the Leizhou peninsula in Guangdong province.
In the stage of adhesion and metamorphosis of the shellfish artificial breeding, the adhesion base needs to be manually put in, so that the larvae can be conveniently adhered. At this stage, the shellfish larvae are the most fragile and lose the most larvae in the actual offspring production. The adhesion and metamorphosis rate of the seedlings are improved, the loss of seedling culture can be reduced, the seedling emergence amount of unit water body is improved, and higher economic benefit is obtained.
Disclosure of Invention
Aiming at the technical problems, the invention provides a method for improving the yield of the pearl oyster seedlings, which improves the metamorphosis rate and the attachment rate of the eyespot larvae which are not metamorphosed and attached by treating the attachment base and inducing the eyespot larvae with glutamic acid, and is simple and easy to operate.
Therefore, the invention adopts the following technical scheme:
a method for improving the yield of pearl oyster seedlings comprises the following steps:
(1) Cleaning the attachment medium, soaking in 0.8-1.5% potassium permanganate solution for 12-24h, cleaning with fresh water for 3-4 times, washing with seawater, spreading on the bottom of culture pond, and adding seawater;
(2) Collecting the eyespot larvae which are not metamorphotic and attached in the nursery pond, and transferring the collected larvae into the pond in the step (1) for cultivation;
(3) Adding glutamic acid into the pond with the non-metamorphosis attached eyespot larvae in the step (2), wherein the concentration of the glutamic acid is 1 x 10 -6 -5×10 -4 M, adding glutamic acid for treatment for 2-48h;
(4) Aerating, changing water and feeding according to a conventional method, and performing shellfish spat attaching operation.
Preferably, the adhesive base is any one of a palm rope, a mesh, and a plastic sheet.
Preferably, the temperature of the seawater is 24-28 ℃.
Preferably, the dropping density of the eyespot larvae in the pool is 0.6-0.7/ml.
Compared with the prior art, the invention has the following beneficial effects:
the invention can obviously improve the metamorphosis rate of the eyespot larvae of the pearl oyster and improve the development level of the pearl oyster by the technical means of attaching base treatment and glutamic acid induction. The method provided by the invention has the advantages of simple and convenient treatment process, low cost, easy operation, convenient popularization and the like.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. For process parameters or conditions not specifically mentioned, it can be carried out with reference to conventional techniques.
Example 1
A method for improving the yield of pearl oyster seedlings comprises the following steps:
(1) Cleaning the attachment base, soaking for 24h by using 1.0% potassium permanganate solution, cleaning for 3 times by using fresh water, finally washing by using seawater, and then spreading at the bottom of a culture pond, adding seawater into the culture pond, and keeping the temperature of the seawater in the culture pond at about 24 ℃;
(2) Collecting the eyespot larvae which are not metamorphosed and attached in the nursery pond, transferring the collected larvae into the water pond in the step (1) for cultivation, wherein the throwing density of the eyespot larvae in the water pond is 0.7/ml;
(3) Adding glutamic acid into the pond with the non-metamorphosis attached eyespot larvae in the step (2), wherein the concentration of the glutamic acid is 1 x 10 -4 M, adding glutamic acid for treating for 12 hours;
(4) Aerating, changing water and feeding according to a conventional method, and performing shellfish spatfall operation.
Example 2
A method for improving the yield of pearl oyster seedlings comprises the following steps:
(1) Cleaning the attachment base, soaking for 24h by using 1.0% potassium permanganate solution, cleaning for 3 times by using fresh water, finally washing by using seawater, and then spreading at the bottom of a culture pond, adding seawater into the culture pond, and keeping the temperature of the seawater in the culture pond at about 24 ℃;
(2) Collecting the eyespot larvae which are not metamorphosed and attached in the nursery pond, transferring the collected larvae into the water pond in the step (1) for cultivation, wherein the throwing density of the eyespot larvae in the water pond is 0.7/ml;
(3) Adding glutamic acid into the pool with the non-metamorphosis attached eyespot larvae in the step (2), wherein the concentration of the glutamic acid is 1 x 10 -4 M, adding glutamic acid for 24 hours;
(4) Aerating, changing water and feeding according to a conventional method, and performing shellfish spatfall operation.
Example 3
A method for improving the yield of pearl oyster seedlings comprises the following steps:
(1) Cleaning an adhesion medium, soaking the adhesion medium in 1.0% potassium permanganate solution for 24 hours, cleaning the adhesion medium with fresh water for 3 times, finally washing the adhesion medium with seawater, then spreading the adhesion medium at the bottom of a culture pond, adding seawater into the culture pond, and keeping the temperature of the seawater in the culture pond at about 24 ℃;
(2) Collecting the eyespot larvae which are not metamorphosed and attached in the nursery pond, transferring the collected larvae into the water pond in the step (1) for cultivation, wherein the throwing density of the eyespot larvae in the water pond is 0.7/ml;
(3) Adding glutamic acid into the pond with the non-metamorphosis attached eyespot larvae in the step (2), wherein the concentration of the glutamic acid is 1 x 10 -4 M, adding glutamic acid for 48 hours;
(4) Aerating, changing water and feeding according to a conventional method, and performing shellfish spatfall operation.
Example 4
A method for improving the yield of pearl oyster seedlings comprises the following steps:
(1) Cleaning the attachment base, soaking for 24h by using 1.0% potassium permanganate solution, cleaning for 3 times by using fresh water, finally washing by using seawater, and then spreading at the bottom of a culture pond, adding seawater into the culture pond, and keeping the temperature of the seawater in the culture pond at about 24 ℃;
(2) Collecting the larvae which are not metamorphosis and attached to the nursery pond, transferring the collected larvae to the pond in the step (1) for breeding, wherein the throwing density of the larvae to the pond is 0.7 per ml;
(3) Adding glutamic acid into the pond with the non-metamorphosis attached eyespot larvae in the step (2), wherein the concentration of the glutamic acid is 1 x 10 -5 M, adding glutamic acid for 24 hours;
(4) Aerating, changing water and feeding according to a conventional method, and performing shellfish spatfall operation.
Example 5
A method for improving the yield of pearl oyster seedlings comprises the following steps:
(1) Cleaning the attachment base, soaking for 24h by using 1.0% potassium permanganate solution, cleaning for 3 times by using fresh water, finally washing by using seawater, and then spreading at the bottom of a culture pond, adding seawater into the culture pond, and keeping the temperature of the seawater in the culture pond at about 24 ℃;
(2) Collecting the larvae which are not metamorphosis and attached to the nursery pond, transferring the collected larvae to the pond in the step (1) for breeding, wherein the throwing density of the larvae to the pond is 0.7 per ml;
(3) Adding glutamic acid into the pond with the non-metamorphosis attached eyespot larvae in the step (2), wherein the concentration of the glutamic acid is 1 x 10 -6 M, adding glutamic acid for 24 hours;
(4) Aerating, changing water and feeding according to a conventional method, and performing shellfish spatfall operation.
Example 6
A method for improving the yield of pearl oyster seedlings comprises the following steps:
(1) Cleaning the attachment base, soaking for 12h by using 1.2% potassium permanganate solution, cleaning for 3 times by using fresh water, finally washing by using seawater, and then spreading at the bottom of a culture pond, adding seawater into the culture pond, and keeping the temperature of the seawater in the culture pond at about 24 ℃;
(2) Collecting the eyespot larvae which are not metamorphosed and attached in the nursery pond, transferring the collected larvae into the water pond in the step (1) for cultivation, wherein the throwing density of the eyespot larvae in the water pond is 0.7/ml;
(3) Adding glutamic acid into the pond with the non-metamorphosis attached eyespot larvae in the step (2), wherein the concentration of the glutamic acid is 1 x 10 -5 M, adding glutamic acid for treating for 12 hours;
(4) Aerating, changing water and feeding according to a conventional method, and performing shellfish spatfall operation.
Example 7
A method for improving the yield of pearl oyster seedlings comprises the following steps:
(1) Cleaning the attachment base, soaking for 20h by using 1.2% potassium permanganate solution, cleaning for 4 times by using fresh water, finally washing by using seawater, and then spreading at the bottom of a culture pond, adding seawater into the culture pond, and keeping the temperature of the seawater in the culture pond at about 24 ℃;
(2) Collecting the eyespot larvae which are not metamorphosed and attached in the nursery pond, transferring the collected larvae into the water pond in the step (1) for cultivation, wherein the throwing density of the eyespot larvae in the water pond is 0.6/ml;
(3) Adding glutamic acid into the pond with the non-metamorphosis attached eyespot larvae in the step (2), wherein the concentration of the glutamic acid is 1 x 10 -5 M, adding glutamic acid for treatment for 12h;
(4) Aerating, changing water and feeding according to a conventional method, and performing shellfish spatfall operation.
Comparative example 1
A method for improving the yield of pearl oyster seedlings comprises the following steps:
(1) Cleaning the attachment base, soaking for 24h by using 1.0% potassium permanganate solution, cleaning for 3 times by using fresh water, finally washing by using seawater, and then spreading at the bottom of a culture pond, adding seawater into the culture pond, and keeping the temperature of the seawater in the culture pond at about 24 ℃;
(2) Collecting the eyespot larvae which are not metamorphosed and attached in the nursery pond, transferring the collected larvae into the water pond in the step (1) for cultivation, wherein the throwing density of the eyespot larvae in the water pond is 0.7/ml;
(3) Aerating, changing water and feeding according to a conventional method, and performing shellfish spatfall operation.
Comparative example 2
A method for improving the yield of pearl oyster seedlings comprises the following steps:
(1) Cleaning the attachment medium, cleaning with fresh water for 3 times, washing with seawater, spreading at the bottom of the culture pond, adding seawater, and maintaining the temperature of the seawater at about 24 deg.C;
(2) Collecting the eyespot larvae which are not metamorphosed and attached in the nursery pond, transferring the collected larvae into the water pond in the step (1) for cultivation, wherein the throwing density of the eyespot larvae in the water pond is 0.7/ml;
(3) Adding glutamic acid into the pond with the non-metamorphosis attached eyespot larvae in the step (2), wherein the concentration of the glutamic acid is 1 x 10 -5 M, adding glutamic acid for 24 hours;
(4) Aerating, changing water and feeding according to a conventional method, and performing shellfish spat attaching operation.
To demonstrate the advantages of the method of the present invention, statistics were made on the eyepoint larva metamorphic attachment rates of examples 1-6 and comparative examples 1-3.
Compared with comparative example 1, the attachment rates of the eyespot larvae of examples 1 to 5 are respectively 3.8 times, 3.7 times, 2.8 times, 2.0 times and 2.2 times of those of the comparative example, which shows that increasing the glutamic acid treatment time and increasing the concentration of glutamic acid can increase the metamorphosis rate of the eyespot larvae of pearl oyster, and the effect of increasing the concentration of glutamic acid on increasing the metamorphosis rate of the eyespot larvae of pearl oyster is more significant. Compared with the comparative example 2, the attachment rate of the eyespot larvae in the example 5 and the example 6 is 2.4 times that of the comparative example 2, and the method for treating the attachment base can also improve the metamorphosis attachment rate of the eyespot larvae of the pearl oyster, so that the yield of the pearl oyster can be improved.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such modifications are intended to be included in the scope of the present invention.
Claims (2)
1. A method for improving the yield of pearl oyster seedlings is characterized by comprising the following steps:
(1) Cleaning an attachment medium, soaking for 12-24h by using 0.8-1.5% potassium permanganate solution, cleaning for 3-4 times by using fresh water, finally washing by using seawater, and then spreading at the bottom of a culture pond, adding seawater into the culture pond, wherein the temperature of the seawater is 24-28 ℃;
(2) Collecting the eyespot larvae which are not metamorphously attached in the nursery pond, and transferring the collected larvae to the water pond in the step (1) for cultivation, wherein the throwing density of the eyespot larvae in the water pond is 0.6/ml;
(3) Adding glutamic acid into the pond with the non-metamorphosis attached eyespot larvae in the step (2), wherein the concentration of the glutamic acid is 1 x 10 -6 -5×10 -4 M, adding glutamic acid for 2-48h；
(4) Aerating, changing water and feeding according to a conventional method, and performing shellfish spatfall operation.
2. The method for improving yield of seedling of pearl oyster as claimed in claim 1, wherein the attaching base is any one of palm rope, grid and plastic sheet.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101703024A (en) * | 2009-07-03 | 2010-05-12 | 浙江海洋学院 | Method and device for cage propagation of Portunus trituberculatus Miers in coastal mudflat pond |
| CN106719188A (en) * | 2016-12-19 | 2017-05-31 | 广东海洋大学 | A kind of method for improving large nacre metamorphosis |
| CN108849658A (en) * | 2018-06-11 | 2018-11-23 | 广东海洋大学 | A method of improving pteria martensii eyebot larvae metamorphosis adhesive rate |
-
2021
- 2021-08-25 CN CN202110979932.5A patent/CN113475439B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101703024A (en) * | 2009-07-03 | 2010-05-12 | 浙江海洋学院 | Method and device for cage propagation of Portunus trituberculatus Miers in coastal mudflat pond |
| CN106719188A (en) * | 2016-12-19 | 2017-05-31 | 广东海洋大学 | A kind of method for improving large nacre metamorphosis |
| CN108849658A (en) * | 2018-06-11 | 2018-11-23 | 广东海洋大学 | A method of improving pteria martensii eyebot larvae metamorphosis adhesive rate |
Non-Patent Citations (1)
| Title |
|---|
| 《Integrated application of transcriptomics and metabolomics provides insights into the larval metamorphosis of pearl oyster (Pinctada fucata martensii)》;Zhang Jiabin 等;《Aquaculture》;20201021;第532卷;第7页第4小节 * |
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