CN106719188A - A kind of method for improving large nacre metamorphosis - Google Patents

A kind of method for improving large nacre metamorphosis Download PDF

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Publication number
CN106719188A
CN106719188A CN201611177654.7A CN201611177654A CN106719188A CN 106719188 A CN106719188 A CN 106719188A CN 201611177654 A CN201611177654 A CN 201611177654A CN 106719188 A CN106719188 A CN 106719188A
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China
Prior art keywords
palm rope
metamorphosis
young
palm
rope
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CN201611177654.7A
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Chinese (zh)
Inventor
邓岳文
王庆恒
杨创业
杜晓东
梁飞龙
李俊辉
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Guangdong Ocean University
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Guangdong Ocean University
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Priority to CN201611177654.7A priority Critical patent/CN106719188A/en
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

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  • Farming Of Fish And Shellfish (AREA)

Abstract

The present invention relates to a kind of method for improving large nacre metamorphosis, belong to technical field of shellfish culture;Concretely comprise the following steps:(1)The 100cm of length 70 is prepared, the palm rope of the 3cm of diameter 2 soaks 10 12 hours with 1% liquor potassic permanganate, then using freshwater soaking 10 12 hours, clean the liquor potassic permanganate of residual;(2)Palm rope is placed in into outdoor tank-culture to soak 12 days, nature seawater, the 15L/min of seawater addition 10 is continuously added;After immersion terminates, the sludge impurity that palm rope adheres to is removed using filtering sea;(3)The ratio of nursery pond eyebot larvae is 50 70%, delivers palm rope, 0.5 1.0/mL of young density, 20 30/m of supply volume3;(4)57 days after young settlement and metamorphosis, attachment base is transferred to another nursery pond and is cultivated;(5)Seed size on palm rope reaches 2 3mm, and attachment base is placed in into 80 mesh mesh bags, transfer natural waterses cultivation;The inventive method can significantly improve the distortion ratio and seed cultivation survival rate of the large nacre young, and with low cost, simple and easy to do.

Description

A kind of method for improving large nacre metamorphosis
Technical field
The present invention relates to a kind of cultivation of the large nacre young, and in particular to a kind of raising large nacre metamorphosis Method, belongs to technical field of shellfish culture.
Background technology
Settlement and metamorphosis are one important growth courses of ocean bivalve shellfish, and floating larva loses in this growth course Face, set life is changed into from life of swimming.Attachment base is one of key factor of influence bivalve shellfish metamorphosis.It is existing Species, material and color there are some researches show the attachment base of attachment base etc. have obvious shadow to the distortion ratio of the bivalve shellfish young Ring, such as using dark colour(Black and brown)Attachment base can improve the distortion ratio of the young.In addition, research show metal from Son, amino acids, neural activity amine chemical substance induction larval metamorphosis.Biomembrane main component is that microbe colony is dwelt the bottom of with Diatom.Existing research reports microbial film and there is obvious influence to the attachment of the ocean bivalve young and metamorphosis process, wherein can Energy reason is that biomembrane can discharge chemical substance, induces larval metamorphosis.
Large nacre is a kind of tropical seashells, mainly moves in the torrid zone, subtropical zone sea area, and NATURAL DISTRIBUTION is in the big profit of Australia The states such as Asia, Philippine, Malaysia, Indonesia it is tropical coastal.Large nacre is for cultivating the main of sea water pearls One of shellfish, the pearl particles of cultivation are big(More than 10mm), pearl thickness, lovely luster, almost occupy whole high-grade both at home and abroad Pearl market.The maximum country of external cultured output is Australia, Indonesia and Philippine, wherein the product of Australia Value highest, accounts for the 50% of world's South sea Pearl output value.In China, large nacre distribution is from Beibu Bay of The South China Sea northeast edge Lezhou Peninsula coastal waters is gone down south, and crosses Qiongzhou Strait, coastal around whole Hainan Island, until the Xisha Islands.The eighties in last century, China's successful incubation large nacre nucleated pearl, but due to large nacre artificial sprout, in sea area, cultivation is survived to the female shellfish that can plant core Rate is very low, does not reach 1%, it is impossible to which batch is obtained plants core mother shellfish, seriously governs China's large nacre and commercially produces.In order to carry Large nacre cultivation survival rate high, Domestic Scientific Research worker has carried out larval culture and sea area cultural technique research, has improved greatly The method of nacre metamorphosis is urgently studied.
The content of the invention
It is an object of the invention to provide a kind of method for improving large nacre metamorphosis, by the use of palm rope as attachment Base, makes attachment primary surface form one layer of biological mould by immersion treatment, induces larval metamorphosis, significantly improves the large nacre young Distortion ratio.
To realize the purpose of foregoing invention, the technical scheme that the present invention takes is as follows:
A kind of method for improving large nacre metamorphosis, comprises the following steps:
(1)Palm rope is soaked with 1% liquor potassic permanganate 10-12 hours, then using freshwater soaking 10-12 hours, cleaning residual Liquor potassic permanganate;
(2)Palm rope is placed in into outdoor tank-culture to soak 1-2 days, nature seawater is continuously added in immersion process, after immersion terminates, The sludge impurity that palm rope adheres to is removed using filtering sea;
(3)The ratio of nursery pond eyebot larvae is 50-70%, delivers palm rope;
(4)5-7 days after young settlement and metamorphosis, palm rope is transferred to another nursery pond and is cultivated;
(5)The seed size of palm rope reaches 2-3mm, and attachment base is placed in into 80 mesh mesh bags, transfer natural waterses cultivation.
Step(1)Described palm rope length specification is 70-100cm, diameter specifications 2-3cm.
Step(2)Described seawater addition 10-15L/min.
Step(3)The quantity that described palm rope is delivered is according to the determination of nursery pond young density, young density 0.5-1.0 Individual/mL, supply volume 20-30/m3
The beneficial effects of the invention are as follows:
(1)With palm rope as attachment base, attachment primary surface is set to form one layer of biological mould by immersion treatment, the young becomes for induction State, is the suitable attachment base of pearl shell because palm rope has relatively rough fibre structure, can significantly improve the metamorphosis of the young Rate.
(2)Plastic plate is the traditional attachment base of pearl shell, when seed size reaches 2-3mm, by seed under attachment scrubbing brush Come, be then transferred to sea area cultivation, due to mechanical injuries reduction weed survival rate.By the use of palm rope as attachment base, it is not necessary to Seed is brushed down, palm rope is directly placed in mesh bag, be transferred to sea area cultivation, it is thus possible to improve seed cultivation survival rate.
(3)Palm rope price, by the use of palm rope as attachment base, can reduce production cost than relatively low, and easy to operate, Easily promote.
Specific embodiment
The present invention is described in further details below by example, these examples are only used for illustrating the present invention, do not limit The scope of the present invention processed.
Embodiment 1
On April 23rd, 2012, Leizhou Hai Wei aquacultures Co., Ltd.Using the natural population collected from Weizhou Island, Beihai City, shellfish Age in age 3-4, shell 16.5-19.3cm long.Choose 8 and select the ripe individuality of sexual gland(Male gonad milky, female gonads are faint yellow), The quantity of male and female is respectively 5 and 3, clears up the attachments such as oyster, barnacle, the lime worm of shellfish body surface attachment.Using the thorn that dries in the shade Swash induction parent's discharge gamete, the hatching of embryonated egg 200L plastic tubs;Incubating oosperm 48h, 1000L glass is transferred to by floating larva Glass steel drum is cultivated, 0.5/mL of young density;Larval culture is carried out according to routine techniques, 2 bait is fed daily, every 1 day Change water 20%;Eyebot larvae ratio reaches 50%, delivers palm rope attachment base;Before dispensing, 1 is soaked using nature seawater is flowed My god, the length 70cm of attachment base, specification 2cm, it is 20 to deliver attachment base quantity;After the young adheres to 7 days, random statistical 3 is attached The seedling quantity of base, average each attachment base seedling quantity 32527.
Embodiment 2
On April 25th, 2013, Leizhou Hai Wei aquacultures Co., Ltd.Cultured population, age in shellfish age 3, shell 14.7-17.5cm long. Choose 10 and select the ripe individuality of sexual gland(Male gonad milky, female gonads are faint yellow), the quantity of male and female is respectively 6 and 4, Clear up the attachments such as oyster, barnacle, the lime worm of shellfish body surface attachment.Parent is soaked using 0.04% serotonin solution 40mins, embryonated egg 200L plastic tub are hatched;Incubating oosperm 48h, is transferred to floating larva 1000L Glass fibre reinforced plastic tubs and cultivates, 1.0/mL of young density;Larval culture is carried out according to routine techniques, 2 bait are fed daily, water 20% was changed every 1 day;Eyespot Larva ratio reaches 70%, delivers palm rope attachment base;Before dispensing, soaked 1 day using nature seawater is flowed, attachment base Length 70cm, specification 2cm, it is 20 to deliver attachment base quantity;After the young adheres to 7 days, the seedling number of 3 attachment bases of random statistical Amount, average each attachment base seedling quantity 34718.
Embodiment 3
On June 12nd, 2013, Leizhou Hai Wei aquacultures Co., Ltd.Cultured population, age in shellfish age 3, shell 15.2-17.8cm long. Choose 6 and select the ripe individuality of sexual gland(Male gonad milky, female gonads are faint yellow), the quantity of male and female is respectively 4 and 2, Clear up the attachments such as oyster, barnacle, the lime worm of shellfish body surface attachment.Using dry in the shade stimulation induction parent's discharge gamete, fertilization Ovum 200L plastic tubs are hatched;Incubating oosperm 48h, is transferred to floating larva 1000L Glass fibre reinforced plastic tubs and cultivates, young density 0.8 Individual/mL;Larval culture is carried out according to routine techniques, 2 bait are fed daily, water 20% was changed every 1 day;Eyebot larvae ratio reaches To 60%, palm rope attachment base is delivered;Before dispensing, soaked 1 day using nature seawater is flowed, the length 70cm of attachment base, rule Lattice 2cm, it is 20 to deliver attachment base quantity;After the young adheres to 7 days, the seedling quantity of 3 attachment bases of random statistical, it is average that each is attached Base seedling quantity 35142.

Claims (4)

1. it is a kind of improve large nacre metamorphosis method, it is characterised in that:Comprise the following steps:
(1)Palm rope is soaked with 1% liquor potassic permanganate 10-12 hours, then using freshwater soaking 10-12 hours, cleaning residual Liquor potassic permanganate;
(2)Palm rope is placed in into outdoor tank-culture to soak 1-2 days, nature seawater is continuously added in immersion process, after immersion terminates, The sludge impurity that palm rope adheres to is removed using filtering sea;
(3)The ratio of nursery pond eyebot larvae is 50-70%, delivers palm rope;
(4)5-7 days after young settlement and metamorphosis, palm rope is transferred to another nursery pond and is cultivated;
(5)The seed size of palm rope reaches 2-3mm, and attachment base is placed in into 80 mesh mesh bags, transfer natural waterses cultivation.
2. the method that a kind of described raising large nacre metamorphosis are required according to right 1, it is characterised in that:Step(1) Described palm rope length specification is 70-100cm, diameter specifications 2-3cm.
3. the method that a kind of described raising large nacre metamorphosis are required according to right 1, it is characterised in that:Step(2) Described seawater addition 10-15L/min.
4. the method that a kind of described raising large nacre metamorphosis are required according to right 1, it is characterised in that:Step(3) The quantity that described palm rope is delivered determines according to nursery pond young density, young density 0.5-1.0/mL, supply volume 20- 30/m3
CN201611177654.7A 2016-12-19 2016-12-19 A kind of method for improving large nacre metamorphosis Pending CN106719188A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108849658A (en) * 2018-06-11 2018-11-23 广东海洋大学 A method of improving pteria martensii eyebot larvae metamorphosis adhesive rate
CN113475439A (en) * 2021-08-25 2021-10-08 广东海洋大学 Method for improving seedling yield of pearl oyster

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101675732A (en) * 2008-09-19 2010-03-24 中国水产科学研究院黄海水产研究所 Ecological treatment and seedling attaching method of shellfish attaching bases
CN102939922A (en) * 2012-11-13 2013-02-27 长岛县科学技术局(长岛县科学技术协会) Holothurian and abalone ecological mixing culture cage netting
CN104719669A (en) * 2015-03-24 2015-06-24 临沂大学 Preparing and feeding method for chlamys farreri larva baits

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101675732A (en) * 2008-09-19 2010-03-24 中国水产科学研究院黄海水产研究所 Ecological treatment and seedling attaching method of shellfish attaching bases
CN102939922A (en) * 2012-11-13 2013-02-27 长岛县科学技术局(长岛县科学技术协会) Holothurian and abalone ecological mixing culture cage netting
CN104719669A (en) * 2015-03-24 2015-06-24 临沂大学 Preparing and feeding method for chlamys farreri larva baits

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108849658A (en) * 2018-06-11 2018-11-23 广东海洋大学 A method of improving pteria martensii eyebot larvae metamorphosis adhesive rate
CN113475439A (en) * 2021-08-25 2021-10-08 广东海洋大学 Method for improving seedling yield of pearl oyster
CN113475439B (en) * 2021-08-25 2022-12-16 广东海洋大学 Method for improving seedling yield of pearl oyster

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