CN102084837B - Sea cucumber seedling culture method - Google Patents
Sea cucumber seedling culture method Download PDFInfo
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- CN102084837B CN102084837B CN2010106044541A CN201010604454A CN102084837B CN 102084837 B CN102084837 B CN 102084837B CN 2010106044541 A CN2010106044541 A CN 2010106044541A CN 201010604454 A CN201010604454 A CN 201010604454A CN 102084837 B CN102084837 B CN 102084837B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
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- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention relates to a sea cucumber seedling culture method which adopts the following technical scheme: young seedling management: controlling the young seedling density at 0.5-1/ml, feeding with ground Sargassum thunbergii Kuntze liquid, changing water everyday, controlling water temperature at 20-25DEG C, and cleaning the bottom every 2-3 days; young sea cucumber management: introducing the young seedlings into a seedling culture pond; taking a colorless and transparent polyethylene corrugated plate as an attaching base, keeping water temperature at 15-23DEG C, keeping illumination at 1500-2500lx, keeping salinity as 2.9-3.2%, keeping dissolved oxygen above 5mg/l, keeping the PH at 7.8-8.5, and controlling culture density at 0.5-1 head/cm<2>; improving water quality with a flowing method in the seedling culture pond; feeding with baits which take Sargassum thunbergii Kuntze powder, gulfweed powder, fish meal, saccharomycete, sea mud, immune polysaccharide and/ or Hormidium flaecidum Br as the main ingredients; feeding with photosynthetic bacteria; purifying water quality; and removing seedling seed dirties. According to the method, the quality and the yield of the sea cucumber seedling culture can be improved.
Description
Technical field
The invention belongs to aquaculture field, relate to particularly a kind of sea cucumber seedling culture method.
Background technology
Development holothruian cultures industry is the important channel that develops living marine resources, and development in recent years is very fast, and cultured area enlarges rapidly, and economic and social benefit is remarkable, has become one of important industry of China's mariculture.Many producers of growing seedlings are engaged in the artificial breeding of stichopus japonicus, however existing seedling-cultivating method: the one,, the output of growing seedlings is unstable, yields poorly; The 2nd,, the seedling body a little less than; The 3rd,, seedling specification difference is larger.Therefore further raising and the industrialization of apostichopus japonicus culture level have been restricted.
Summary of the invention
In order to overcome the above problems, the purpose of this invention is to provide a kind of sea cucumber seedling culture method, to improve output and the quality of sea cucumber seed.
The objective of the invention is to be achieved through the following technical solutions: a kind of sea cucumber seedling culture method is as follows:
(1) management is stored up in parent participation:
Holding pond adopts cement tub, adopts the individual layer floating cage to support temporarily, and cultivating density is 15～25/m
3, 1.2～1.5 meters of the depth of waters, 16～18 ℃ of water temperatures, salinity 29～32 ‰, pH value is 7.8～8.5, illumination 500～1000lx, dissolved oxygen is at 5 ㎎/more than l; Parent participation every day by 4% minute of its body weight sooner or later twice sargassum thunbergii of throwing something and feeding grind liquid; Sooner or later respectively change water once every day, and each quantity of exchanged water is 1/3～1/2, changes water temperature difference and is not more than 1 ℃;
(2) spawning and fertilization:
The density of laying eggs is not more than 50/㎡, in time parent participation shifted out after the parent participation spawning and fertilization, and with repeatedly washing ovum repeatedly through the clean seawater of sand filtration sterilization, until Chi Shui becomes clear;
(3) hatching:
Utilize phreatic water cooling tank maritime interior waters, requirement for environmental conditions is 17～20 ℃ of water temperatures, salinity 29～32 ‰, and pH value 7.8～8.5, illumination 500～1000lx, dissolved oxygen is 5 ㎎/more than l, hatching density is 20～50/ml;
(4) seedling management:
Seedling density is controlled at 0.5～1/ml, grinds liquid with sargassum thunbergii and throws something and feeds, and changes water every day 2 times, changes water 1/2～1/3 at every turn, stirs gently up and down Chi Shui 1 time every one hour with water shoveling board, and water temperature is controlled at 20～25 ℃, 2-3 days with the siphon pipe clear end once;
(5) young ginseng management:
1) when growth of seedling when the five former bases of tentacle or seedling 20%～30% occurring and develop into doliolaria, import nursery pond; Select water white polyethylene corrugated plate to do adherance, water temperature remains on 15～23 ℃, and illumination is 500～1000lx, and salinity is 29～32 ‰, and dissolved oxygen is cultivated density and is controlled at 0.5～1 Tou/㎝ 5 ㎎/more than l, pH value is 7.8～8.5
2
2) nursery pond adopts the flowing water method to improve water quality, and every day, flowing water was 4～6 times, and each 2～3 hours, every day, streamflow was 3～4 times of nursery pond water body; Soil pick-up was 1 time in every 2～3 days, and two weeks falls pond 1 time;
3) bait of throwing something and feeding, the bait main component is sargassum thunbergii powder, sargassum powder, fish meal, saccharomycete, ooze, immune polysaccharide and/or steel wire algae;
4) throw something and feed photosynthetic bacteria to improve the micro-ecological environment of nursery pond, purify water, strengthen young ginseng resistance against diseases;
(6) the removing seed is dirty:
In seedling and young ginseng stage, it is dirty that adherance grows a large amount of salt loud, high-pitched sounds etc., in sea cucumber when the falling the pond clear end, at first prepares a basin seawater, and about 5 jin of seedlings are put into basin, rotates basin water by hand, in the middle of dirty concentrating on, fishes for 15 centimetre of 100 little fishing net of order of diameter.
Above-mentioned sea cucumber seedling culture method, adherance sodium hydroxide solution with 0.5 ‰-1.0 ‰ before input soaked 2～3 days, then wash with liquor potassic permanganate and brush one times, rinse well with filtering sea again, put into nursery pond after washing and inoculate benthic diatom, stir transfixion, be inverted adherance next day, the inoculation another side.
The invention has the beneficial effects as follows: the present invention utilizes phreatic water cooling tank maritime interior waters in the hatching stage, has avoided water temperature over-high, and good seedling living environment is provided, and has improved survival rate, and survival rate can reach 90%, and what assurance was grown seedlings carries out smoothly.The photosynthetic bacteria of throwing something and feeding in the pond is effectively improved the micro-ecological environment of nursery pond, purifies water, and strengthens young ginseng resistance against diseases.Selection water white transparency, the polyethylene corrugated plate that ripple is high, area is large are done adherance, are beneficial to and adhere to and ingest.Adopt special method removing seed dirty, reduce water pollution, promote growth and survive.Adopt the present invention, the one, growth time is long.Owing to utilizing underground sea water regulation and control water temperature, guaranteed that seedling grows at the matters temperature, avoided the aestivation winter of seedling to bite, can extend wheat harvesting period in vegetative period 5, thus long fast, individual large of seedling, the young ginseng of the 2cm 4cm-6cm that can grow up, large individuality can reach 8cm.The 2nd, survival rate is high, and is good in economic efficiency.Because the living condition to young ginseng has been carried out full artificial regulatory, so survival rate of seedlings is high, generally can reach 30% left and right.
Embodiment
EmbodimentA kind of sea cucumber seedling culture method is as follows:
(1) management is stored up in parent participation:
Holding pond adopts cement tub, adopts the individual layer floating cage to support temporarily, and cultivating density is 15～25/m
3, 1.2～1.5 meters of the depth of waters, 16～18 ℃ of water temperatures, salinity 29～32 ‰, pH value is 7.8～8.5, illumination 500～1000lx, dissolved oxygen is at 5 ㎎/more than l; Parent participation every day by 4% minute of its body weight sooner or later twice sargassum thunbergii of throwing something and feeding grind liquid, throw 30% the morning, throw 70% afternoon.Sooner or later respectively change water once every day, and each quantity of exchanged water is 1/3～1/2, changes water temperature difference and is not more than 1 ℃, in time removes excreta and residual bait at the bottom of the pond;
(2) spawning and fertilization:
The density of laying eggs is not more than 50/㎡, in time parent participation shifted out after the parent participation spawning and fertilization, and with repeatedly washing ovum repeatedly through the clean seawater of sand filtration sterilization, until Chi Shui becomes clear; The method of adopting ovum can adopt to heat up and induce and dark dry technique,
(3) hatching:
Utilize phreatic water cooling tank maritime interior waters, requirement for environmental conditions is 17～20 ℃ of water temperatures, salinity 29～32 ‰, and pH value 7.8～8.5, illumination 500～1000lx, dissolved oxygen be at 5 ㎎/more than l, the continuous micro inflation, hatching density is 20～50/ml;
(4) seedling management:
To carry out seed selection when embryonic development during to the microtia young, drag choosing or with siphon pipe siphon seed selection, Seedling density is controlled at 0.5～1/ml, grinds liquid with sargassum thunbergii and throws something and feeds, and throws something and feeds and adheres to few principle of throwing diligent throwing with NX～79 nylon mesh; Generally do not fall the pond in the floating larva nurturing period, because falling the pond all can damage the young at every turn, mainly improve water quality by changing water: change water every day 2 times, change water 1/2～1/3 at every turn, for the young is evenly distributed, improve the survival rate of the young, stirred gently up and down Chi Shui 1 time every one hour with water shoveling board; Water temperature is controlled at 20～25 ℃, 2～3 days with the siphon pipe clear end once, accomplish thoroughly totally, the excreta of residual bait, protozoa and the young at the bottom of the pond etc. is cleared out;
(5) young ginseng management:
1) when growth of seedling when the five former bases of tentacle or seedling 20%～30% occurring and develop into doliolaria, import nursery pond; Select water white polyethylene corrugated plate to do adherance, the sodium hydroxide solution with 0.5 ‰～1.0 ‰ before adherance is thrown in soaked 2～3 days, then washed with liquor potassic permanganate to brush one times, then rinsed well with filtering sea, removed medicine, greasy dirt and foul.Can put into after washing and cultivate the pond and inoculate benthic diatom.The algae kind is take small-sized boat-shaped algae, avette algae and rhombus algae as main, can take from pool wall, cuts down frame, on reef and tangleweed.During inoculation, adherance is tiled in horizontal direction and cultivates in the pond, add appropriate fresh filtering sea, then access the algae kind, stir transfixion.Be inverted adherance next day, and the inoculation another side uses the same method.The thing that should be noted that, in time sparse after the adherance inoculation, make its vertical placement, changed water once in every 1～3 day, change 1/2～1/3 at every turn, and press quantity of exchanged water fertilising (nitrogen 10～20 * 10
-6, phosphorus 1～2 * 10
-6, silicon 1.0 * 10
-6, iron 0.1 * 10
-6).Water temperature remains on 15～23 ℃, and illumination is 500～1000lx, and salinity is 29～32 ‰, and dissolved oxygen is cultivated density and is controlled at 0.5～1 Tou/㎝ 5 ㎎/more than l, pH value is 7.8～8.5
2
2) nursery pond adopts the flowing water method to improve water quality, and every day, flowing water was 4～6 times, and each 2～3 hours, every day, streamflow was 3～4 times of nursery pond water body; Soil pick-up was 1 time in every 2～3 days, and two weeks falls pond 1 time;
3) bait of throwing something and feeding, the bait main component is sargassum thunbergii powder, sargassum powder, fish meal, saccharomycete, ooze, immune polysaccharide and/or steel wire algae;
4) throw something and feed photosynthetic bacteria to improve the micro-ecological environment of nursery pond, purify water, strengthen young ginseng resistance against diseases;
(6) the removing seed is dirty:
In seedling and young ginseng stage, it is dirty that adherance grows a large amount of salt loud, high-pitched sounds etc., in sea cucumber when the falling the pond clear end, at first prepares a basin seawater, and about 5 jin of seedlings are put into basin, rotates basin water by hand, in the middle of dirty concentrating on, fishes for 15 centimetre of 100 little fishing net of order of diameter.
Adopt said method, owing to utilizing underground sea water regulation and control water temperature, guaranteed that seedling grows through practice at the matters temperature, avoided the aestivation winter of seedling to bite, can extend wheat harvesting period in vegetative period 5, so seedling length is fast, individual large, the young ginseng of the 2cm 4cm-6cm that can grow up, large individuality can reach 8cm.The 2nd, survival rate is high, and is good in economic efficiency.Because the living condition to young ginseng has been carried out full artificial regulatory, so survival rate of seedlings is high, generally can reach 30% left and right.
Claims (1)
1. sea cucumber seedling culture method is characterized in that method is as follows:
(1) management is stored up in parent participation:
Holding pond adopts cement tub, adopts the individual layer floating cage to support temporarily, and cultivating density is 15～25/m
3, 1.2～1.5 meters of the depth of waters, 16～18 ℃ of water temperatures, salinity 29～32 ‰, pH value is 7.8～8.5, illumination 500～1000lx, dissolved oxygen is at 5 ㎎/more than l; Parent participation every day by 4% minute of its body weight sooner or later twice sargassum thunbergii of throwing something and feeding grind liquid; Sooner or later respectively change water once every day, and each quantity of exchanged water is 1/3～1/2, changes water temperature difference and is not more than 1 ℃;
(2) spawning and fertilization:
The density of laying eggs is not more than 50/㎡, in time parent participation shifted out after the parent participation spawning and fertilization, and with repeatedly washing ovum repeatedly through the clean seawater of sand filtration sterilization, until Chi Shui becomes clear;
(3) hatching:
Utilize phreatic water cooling tank maritime interior waters, requirement for environmental conditions is 17～20 ℃ of water temperatures, salinity 29～32 ‰, and pH value 7.8～8.5, illumination 500～1000lx, dissolved oxygen is 5 ㎎/more than l, hatching density is 10～20/ml;
(4) seedling management:
Seedling density is controlled at 0.5～1/ml, grinds liquid with sargassum thunbergii and throws something and feeds, and changes water every day 2 times, changes water 1/2～1/3 at every turn, stirs gently up and down Chi Shui 1 time every one hour with water shoveling board, and water temperature is controlled at 20～25 ℃, 2～3 days with the siphon pipe clear end once;
(5) young ginseng management:
1) when growth of seedling when the five former bases of tentacle or seedling 20%～30% occurring and develop into doliolaria, import nursery pond; Select water white polyethylene corrugated plate to do adherance, water temperature remains on 15～23 ℃, and illumination is 500～1000lx, and salinity is 29～32 ‰, and dissolved oxygen is cultivated density and is controlled at 0.5～1 Tou/㎝ 5 ㎎/more than l, pH value is 7.8～8.5
2
Adherance sodium hydroxide solution with 0.5 ‰-1.0 ‰ before input soaked 2～3 days, then wash with liquor potassic permanganate and brush one times, rinse well with filtering sea again, put into nursery pond after washing and inoculate benthic diatom, stir, transfixion, be inverted adherance next day, the inoculation another side;
2) nursery pond adopts the flowing water method to improve water quality, and every day, flowing water was 4～6 times, and each 2～3 hours, every day, streamflow was 3～4 times of nursery pond water body; Soil pick-up was 1 time in every 2～3 days, and two weeks falls pond 1 time;
3) bait of throwing something and feeding, the bait main component is sargassum thunbergii powder, sargassum powder, fish meal, saccharomycete, ooze, immune polysaccharide and/or steel wire algae;
4) throw something and feed photosynthetic bacteria to improve the micro-ecological environment of nursery pond, purify water, strengthen young ginseng resistance against diseases;
(6) the removing seed is dirty:
In seedling and young ginseng stage, it is dirty that adherance grows a large amount of salt loud, high-pitched sounds, in sea cucumber when the falling the pond clear end, at first prepares a basin seawater, and about 5 jin of seedlings are put into basin, rotates basin water by hand, in the middle of dirty concentrating on, fishes for 15 centimetre of 100 little fishing net of order of diameter.
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| CN2010106044541A CN102084837B (en) | 2010-12-24 | 2010-12-24 | Sea cucumber seedling culture method |
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| CN2010106044541A CN102084837B (en) | 2010-12-24 | 2010-12-24 | Sea cucumber seedling culture method |
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Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102550453A (en) * | 2012-01-11 | 2012-07-11 | 中国海洋大学 | Sea cucumber seed raising method using bacterial membrane attachment substances |
| CN102870712A (en) * | 2012-09-20 | 2013-01-16 | 黄思睿 | Sea cucumber seedling raising method |
| CN103960171B (en) * | 2013-01-28 | 2015-12-23 | 中国科学院海洋研究所 | Bohadschia argus Jaeger fingerlings artificial culturing method |
| CN104365509A (en) * | 2013-08-12 | 2015-02-25 | 大连长海海滨水产有限公司 | Sea cucumber culturing method |
| CN104396814B (en) * | 2014-10-30 | 2017-02-15 | 厦门大学 | Indoor bostrichthys sinensis fry intermediate rearing method |
| CN104719201B (en) * | 2015-03-19 | 2018-08-28 | 山东省海洋生物研究院 | A kind of imitative apostichopus japonicus culture method of multilayer slot type |
| CN105325334A (en) * | 2015-10-31 | 2016-02-17 | 颍上县润海畜禽养殖有限公司 | Method for artificially hatching sea cucumber eggs |
| CN107372234A (en) * | 2017-08-23 | 2017-11-24 | 桂平市蒙圩镇火炎种养专业合作社 | A kind of method that sea cucumbers are cultivated with well water |
| CN107494354A (en) * | 2017-09-12 | 2017-12-22 | 佛山推启农业研究院（普通合伙） | A kind of holothurian culture method |
| CN110810288A (en) * | 2019-12-19 | 2020-02-21 | 大连宝发海珍品有限公司 | High-temperature sea cucumber cultivation method |
| CN113115729A (en) * | 2020-01-10 | 2021-07-16 | 大连鑫玉龙海洋生物种业科技股份有限公司 | Method for feeding sea cucumber seedlings by mixing granulated feed and fermented feed |
| CN112655610B (en) * | 2020-12-18 | 2022-08-05 | 广东省农业科学院动物科学研究所 | Efficient cultivation method for tropical sea cucumber seedlings |
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| CN101720685A (en) * | 2008-10-30 | 2010-06-09 | 大连水产学院 | High strength selecting and cultivating method of excellent stichopus japonicus seeds |
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