CN103478056B - Method for improving emission ratio of pinctada maxima parent gamete - Google Patents
Method for improving emission ratio of pinctada maxima parent gamete Download PDFInfo
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- CN103478056B CN103478056B CN201310501653.3A CN201310501653A CN103478056B CN 103478056 B CN103478056 B CN 103478056B CN 201310501653 A CN201310501653 A CN 201310501653A CN 103478056 B CN103478056 B CN 103478056B
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- parent
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention relates to a method for improving the emission ratio of pinctada maxima parent gametes and belongs to the technical field of shellfish culture. The method concretely comprises the following steps of: selecting individuals with higher activity and plump gonads from a 3-year or 4-year natural population or cultured population as parents; cleaning attachments from the surfaces of shell bodies, and soaking for 1-2 minutes by utilizing 0.02% potassium permanganate solution; soaking the parents in 0.02%-0.05% 5-hydroxytryptamine solutions for 30-50 minutes. The method disclosed by the invention can effectively and fast promote the emission of the pinctada maxima parent gametes and improve the survival rate of the parents and has the advantages of easiness and convenience in operation, low cost, easiness in popularization, and the like.
Description
Technical field
The present invention relates to a kind of technology of hastening parturition of pearl shell, be specifically related to a kind of method improving emission ratio of pinctada maxima parent gamete, belong to technical field of shellfish culture.
Background technology
Large nacre (
pinctada maximajameson) Mollusca (Mollusca) is under the jurisdiction of, Bivalvia (Bivalvia), Anisomyaria (Anisomyaria), Pteriidae (Pteriidae), Pinctada (Pinctada), is commonly called as silver-colored lip shellfish (Silver lip pearl oyster) and golden lip shellfish (Golden lip pearl oyster) abroad.Move in the torrid zone, sea area, subtropics, natural distribution is coastal in the torrid zone of the states such as Australia, Philippine, Malaysia, Indonesia, and it is coastal that the resource of China's large nacre is mainly distributed in the Lezhou Peninsula, Hainan Island and the Xisha Islands.Utilize the pearl that large nacre is cultivated, gloss is very strong, beautiful appearance, and diameter is general all at 1 more than cm, and after processing, every value can reach 3 000-4 000 dollar, high-quality person can reach millions of dollars, is therefore to produce one of optimal pearl shell of large-scale sea water pearls.
Large nacre artificial nursery and technology of growing cultured pearls, just progressively grew up at nearly 40 years.Eighties of last century seventies, China scientific research personnel obtains large nacre artificial nursery success first in pearl farm, Xian Hai mound, Lingshui, Hainan Island, creates indoor pond and cultivates seedling and sea area Feeding Technique.Through the research of nearly 40 years, China's large nacre seedling growing process was ripe gradually.One of large nacre nursery key link is that parent induces discharge gamete, at present at seedling raising process, normal employing conventional method induction parent discharges gamete, mainly comprising of tradition induction: 1) dissect sexual gland, artificial insemination, this method major defect: the young floating ability of cultivation, ontogeny is irregular, and abnormal rate is high, and water quality is restive; Loss parent management; 2) dry in the shade stimulation; 3) combine to dry in the shade and to stimulate with temperature difference.2) and the 3) defect of method: induction emission index is low, after close shellfish induction, lethality is high, and the close shellfish lethality that especially maturity is good is high.Patent of invention: application number 200710168056.8 provides a kind of simple to operate, the artificial sprout continental rise controlled water body continental rise controlled water body intermediate cultivate method that aquaculture cost is lower, solve the problem that large nacre cultivation survival rate is low, positive effect is served to the cultivation recovering large nacre, what this method solve that external condition cultivates large nacre affects problem, improve the problem of its survival rate simultaneously, the cultivation of large nacre is had great importance, but at present by improving parent's gamete emission index, the research improving survival rate have not been reported, therefore, the gamete discharge method that research and development large nacre parent is suitable for, effectively can improve the hatching larvae rate of large nacre, improve the seed amount of unit of water body.The present invention mainly utilizes serotonin solution to soak parent, effectively can improve gamete emission index and the survival rate of parent, simple and easy to do, easily promotes.
Summary of the invention
The object of the present invention is to provide a kind of method improving emission ratio of pinctada maxima parent gamete, effectively can improve gamete emission index and the survival rate of parent, and have easy and simple to handle, the advantages such as easy popularization.
For realizing the object of foregoing invention, the technical scheme that the present invention takes is as follows:
Improve a method for emission ratio of pinctada maxima parent gamete, comprise the steps:
(1) parent selects: select the long 14.0-20.0cm of shell from the natural population in 3 ages or 4 ages or cultured population, the individuality that vigor is comparatively strong, sexual gland is full is as parent;
(2) parent's cleaning: by parent transfer to indoor raising of seedling field, the attachment of cleaning shellfish surface, and shellfish body is carried out disinfection; Utilize 0.02% disinfecting solution of potassium permanganate shellfish body 1-2min;
(3) immersion treatment: serotonin solution parent being immersed in 0.02-0.05%, soak time 30-50min.
Described parent's sexual gland is full; Described sterilization refers to 0.02% disinfecting solution of potassium permanganate shellfish body 1-2min; The compound method of described serotonin solution is: weigh 0.2-0.5g5-hydroxytryptamine, be dissolved in 10L filtering sea, seawater salinity 28-30ppt.
Beneficial effect of the present invention is:
(1) gamete emission index and the survival rate of parent can be improved;
(2) advantages such as the method has easy and simple to handle, with low cost, easy popularization.
Embodiment
Explain the present invention further below in conjunction with embodiment, but embodiment does not limit in any form to the present invention.
Embodiment 1
On April 23rd, 2012, Leizhou Hai Wei aquaculture Co., Ltd.Utilize the natural population of collecting from Weizhou Island, Beihai City, shellfish 3-4 in age age, the long 16.5-19.3cm of shell.Choose 8 and select the individuality of gonad maturity (male gonad milky, female gonads are faint yellow), the quantity of male and female is respectively 5 and 3, the attachment such as oyster, barnacle, lime worm of cleaning shellfish surface attachment.Utilize 0.02% disinfecting solution of potassium permanganate shellfish body 1min; Weigh 0.2g5-hydroxytryptamine, be dissolved in 10L sand filtration seawater (salinity 29.5ppt), the serotonin solution of compound concentration 0.02%; Parent is put into the solution of preparation, soak time is 30min, micro-inflation; The emission index (having discharged parent/total parent of gamete) of parent is 100%, and test under microscope ovum size is even, and proterties is perfectly round, fertilization rate 98%.
Embodiment 2
On April 25th, 2013, Leizhou Hai Wei aquaculture Co., Ltd.Cultured population, shellfish age in age 3, the long 14.7-17.5cm of shell.Choose 10 and select the individuality of gonad maturity (male gonad milky, female gonads are faint yellow), the quantity of male and female is respectively 6 and 4, the attachment such as oyster, barnacle, lime worm of cleaning shellfish surface attachment.Utilize 0.02% disinfecting solution of potassium permanganate shellfish body 1.5min; Weigh 0.50g5-hydroxytryptamine, be dissolved in 10L sand filtration seawater (salinity 30.2ppt), the serotonin solution of compound concentration 0.05%; Parent is put into the solution of preparation, soak time is 50min, micro-inflation; The emission index of parent is 100%, and test under microscope ovum size is even, and proterties is perfectly round, fertilization rate 98.4%.
Embodiment 3
On June 12nd, 2013, Leizhou Hai Wei aquaculture Co., Ltd.Cultured population, shellfish age in age 3, the long 15.2-17.8cm of shell.Choose 6 and select the individuality of gonad maturity (male gonad milky, female gonads are faint yellow), the quantity of male and female is respectively 4 and 2, the attachment such as oyster, barnacle, lime worm of cleaning shellfish surface attachment.Utilize 0.02% disinfecting solution of potassium permanganate shellfish body 1min; Weigh 0.3g5-hydroxytryptamine, be dissolved in 10L sand filtration seawater (salinity 29.5ppt), the serotonin solution of compound concentration 0.04%; Parent is put into the solution of preparation, soak time is 40min, micro-inflation; The emission index of parent is 100%, and test under microscope ovum size is even, and proterties is perfectly round, fertilization rate 97.4%.
Embodiment 4
On July 25th, 2013, Leizhou Hai Wei aquaculture Co., Ltd.Cultured population, shellfish age in age 3, the long 14.5-18.5cm of shell.Choose 8 and select the individuality of gonad maturity (male gonad milky, female gonads are faint yellow), the quantity of male and female is respectively 5 and 3, the attachment such as oyster, barnacle, lime worm of cleaning shellfish surface attachment.Utilize 0.02% disinfecting solution of potassium permanganate shellfish body 2min; Weigh 0.50g5-hydroxytryptamine, be dissolved in 10L sand filtration seawater (salinity 30.2ppt), the serotonin solution of compound concentration 0.05%; Parent is put into the solution of preparation, soak time is 30min, micro-inflation; The emission index of parent is 100%, and test under microscope ovum size is even, and proterties is perfectly round, fertilization rate 98.6%.
Embodiment 5
On August 18th, 2013, Leizhou Hai Wei aquaculture Co., Ltd.Cultured population, shellfish age in age 3, the long 15.5-17.9cm of shell.Choose 6 and select the individuality of gonad maturity (male gonad milky, female gonads are faint yellow), the quantity of male and female is respectively 4 and 2, the attachment such as oyster, barnacle, lime worm of cleaning shellfish surface attachment.Utilize 0.02% disinfecting solution of potassium permanganate shellfish body 1min; Weigh 0.3g5-hydroxytryptamine, be dissolved in 10L sand filtration seawater (salinity 29.5ppt), the serotonin solution of compound concentration 0.04%; Parent is put into the solution of preparation, soak time is 40min, micro-inflation; The emission index of parent is 100%, and test under microscope ovum size is even, and proterties is perfectly round, fertilization rate 97.8%.
Claims (2)
1. improve a method for emission ratio of pinctada maxima parent gamete, it is characterized in that: comprise the steps:
(1) parent selects: select the long 14.0-20.0cm of shell from the natural population in 3 ages or 4 ages or cultured population, the individuality that vigor is comparatively strong, sexual gland is full is as parent;
(2) parent's cleaning: by parent transfer to indoor raising of seedling field, the attachment of cleaning shellfish surface, and shellfish body is carried out disinfection; Utilize 0.02% disinfecting solution of potassium permanganate shellfish body 1-2min;
(3) immersion treatment: the serotonin solution immersion 30-50min by parent's mass concentration being 0.02-0.05%.
2. the method for described a kind of raising emission ratio of pinctada maxima parent gamete is required according to right 1, it is characterized in that: the compound method of the serotonin solution described in step (3) is: weigh 0.2-0.5g5-hydroxytryptamine, be dissolved in 10L filtering sea, seawater salinity 28-30ppt.
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| CN107173287A (en) * | 2016-07-03 | 2017-09-19 | 李育培 | A kind of induced spawning method of giant clam |
| CN106577413B (en) * | 2016-11-01 | 2019-07-12 | 海南大学 | A kind of artificial induction's oviposition method of giant clam |
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