CN103478056A - Method for improving emission ratio of pinctada maxima parent gamete - Google Patents
Method for improving emission ratio of pinctada maxima parent gamete Download PDFInfo
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- CN103478056A CN103478056A CN201310501653.3A CN201310501653A CN103478056A CN 103478056 A CN103478056 A CN 103478056A CN 201310501653 A CN201310501653 A CN 201310501653A CN 103478056 A CN103478056 A CN 103478056A
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- parent
- gamete
- emission index
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- large nacre
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention relates to a method for improving the emission ratio of pinctada maxima parent gametes and belongs to the technical field of shellfish culture. The method concretely comprises the following steps of: selecting individuals with higher activity and plump gonads from a 3-year or 4-year natural population or cultured population as parents; cleaning attachments from the surfaces of shell bodies, and soaking for 1-2 minutes by utilizing 0.02% potassium permanganate solution; soaking the parents in 0.02%-0.05% 5-hydroxytryptamine solutions for 30-50 minutes. The method disclosed by the invention can effectively and fast promote the emission of the pinctada maxima parent gametes and improve the survival rate of the parents and has the advantages of easiness and convenience in operation, low cost, easiness in popularization, and the like.
Description
Technical field
The present invention relates to a kind of technology of hastening parturition of pearl shell, be specifically related to a kind of method that improves large nacre parent gamete emission index, belong to the shellfish culture technical field.
Background technology
Large nacre (
pinctada maximajameson) be under the jurisdiction of Mollusca (Mollusca), Bivalvia (Bivalvia), Anisomyaria (Anisomyaria), Pteriidae (Pteriidae), Pinctada (Pinctada), be commonly called as silver-colored lip shellfish (Silver lip pearl oyster) and golden lip shellfish (Golden lip pearl oyster) abroad.Move in the torrid zone, sea area, subtropics, natural distribution is coastal in the torrid zone of the states such as Australia, Philippine, Malaysia, Indonesia, and it is coastal that the resource of China's large nacre mainly is distributed in the Lezhou Peninsula, Hainan Island and the Xisha Islands.The pearl that utilizes large nacre to cultivate, gloss is very strong, color is attractive in appearance, and diameter is generally all more than 1 cm, and after processing, every worth up to 3 000-4 is 000 dollar, high-quality person can reach up to ten thousand dollars, is therefore to produce one of optimal pearl shell of large-scale sea water pearls.
The large nacre artificial technology of growing seedlings and grow cultured pearls, just progressively grew up at nearly 40 years.Eighties of last century seventies, China scientific research personnel obtains first large nacre artificial in pearl farm, Xian Hai mound, Lingshui, Hainan Island and grows seedlings successfully, has created that seedling is cultivated in indoor pond and technology is formed in sea area.Through the research of nearly 40 years, China's large nacre seedling growing process was ripe gradually.Large nacre one of the key link of growing seedlings is that the parent induces the discharge gamete, at present at seedling raising process, the normal conventional method that adopts induces the parent to discharge gamete, what tradition was induced mainly comprises: 1) dissect sexual gland, artificial insemination, this method major defect: the young floating ability of cultivation, ontogeny is irregular, and abnormal rate is high, and water quality is restive; Loss parent resource; 2) dry in the shade stimulation; 3) in conjunction with drying in the shade, with temperature difference, stimulate.2) and the 3) defect of method: induce emission index low, close shellfish induces rear lethality high, and the close shellfish lethality that especially maturity is good is high.Patent of invention: application number 200710168056.8 provides a kind of simple to operate, the artificial sprout continental rise controlled water body continental rise controlled water body intermediate cultivate method that aquaculture cost is lower, solved the low problem of large nacre cultivation survival rate, the cultivation that recovers large nacre has been played to positive effect, the method has solved the problem that affects that external condition is cultivated large nacre, improved the problem of its survival rate simultaneously, cultivation to large nacre has great importance, but at present by improving parent's gamete emission index, the research that improves survival rate have not been reported, therefore, the suitable gamete discharge method of research and development large nacre parent, can effectively improve the hatching larvae rate of large nacre, improve the seed amount of unit of water body.The present invention mainly utilizes serotonin solution to soak the parent, can effectively improve parent's gamete emission index and survival rate, simple and easy to do, easily promotes.
Summary of the invention
The object of the present invention is to provide a kind of method that improves large nacre parent gamete emission index, can effectively improve parent's gamete emission index and survival rate, and there are the advantages such as easy and simple to handle, easy popularization.
For realizing the purpose of foregoing invention, the technical scheme that the present invention takes is as follows:
A kind of method that improves large nacre parent gamete emission index, comprise the steps:
(1) parent selects: select the long 14.0-20.0cm of shell from natural population or the cultured population in 3 ages or 4 ages, the individuality that vigor is strong, sexual gland is full is as the parent;
(2) parent's cleaning: parent transfer, to the indoor raising of seedling field, is cleared up to the attachment of shellfish surface, and the shellfish body is carried out disinfection; Utilize 0.02% disinfecting solution of potassium permanganate shellfish body 1-2min;
(3) immersion treatment: the parent is immersed in to the serotonin solution of 0.02-0.05%, soak time 30-50min.
Described parent's sexual gland is full; Described sterilization refers to 0.02% disinfecting solution of potassium permanganate shellfish body 1-2min; The compound method of described serotonin solution is: weighing 0.2-0.5g5-hydroxytryptamine is dissolved in the 10L filtering sea, seawater salinity 28-30ppt.
Beneficial effect of the present invention is:
(1) can improve parent's gamete emission index and survival rate;
(2) the method has the advantages such as easy and simple to handle, with low cost, easy popularization.
Embodiment
Further explain the present invention below in conjunction with embodiment, but embodiment does not limit in any form to the present invention.
Embodiment 1
On April 23rd, 2012, sea, Leizhou prestige aquaculture Co., Ltd.The natural population that utilization is collected from Weizhou Island, Beihai City, shellfish 3-4 in age age, the long 16.5-19.3cm of shell.Choose 8 individualities that select gonad maturity (male gonad milky, female sexual gland faint yellow), the quantity of male and female is respectively 5 and 3, the attachments such as the oyster that cleaning shellfish surface is adhered to, barnacle, lime worm.Utilize 0.02% disinfecting solution of potassium permanganate shellfish body 1min; Weighing 0.2g5-hydroxytryptamine, be dissolved in 10L sand filtration seawater (salinity 29.5ppt), the serotonin solution of compound concentration 0.02%; The parent is put into to the solution of preparation, soak time is 30min, micro-inflation; Parent's emission index (having discharged the parent of gamete/total parent) is 100%, and evenly, proterties is perfectly round, fertilization rate 98% for test under microscope ovum size.
Embodiment 2
On April 25th, 2013, sea, Leizhou prestige aquaculture Co., Ltd.Cultured population, shellfish 3 ages of age, the long 14.7-17.5cm of shell.Choose 10 individualities that select gonad maturity (male gonad milky, female sexual gland faint yellow), the quantity of male and female is respectively 6 and 4, the attachments such as the oyster that cleaning shellfish surface is adhered to, barnacle, lime worm.Utilize 0.02% disinfecting solution of potassium permanganate shellfish body 1.5min; Weighing 0.50g5-hydroxytryptamine, be dissolved in 10L sand filtration seawater (salinity 30.2ppt), the serotonin solution of compound concentration 0.05%; The parent is put into to the solution of preparation, soak time is 50min, micro-inflation; Parent's emission index is 100%, and evenly, proterties is perfectly round, fertilization rate 98.4% for test under microscope ovum size.
Embodiment 3
On June 12nd, 2013, sea, Leizhou prestige aquaculture Co., Ltd.Cultured population, shellfish 3 ages of age, the long 15.2-17.8cm of shell.Choose 6 individualities that select gonad maturity (male gonad milky, female sexual gland faint yellow), the quantity of male and female is respectively 4 and 2, the attachments such as the oyster that cleaning shellfish surface is adhered to, barnacle, lime worm.Utilize 0.02% disinfecting solution of potassium permanganate shellfish body 1min; Weighing 0.3g5-hydroxytryptamine, be dissolved in 10L sand filtration seawater (salinity 29.5ppt), the serotonin solution of compound concentration 0.04%; The parent is put into to the solution of preparation, soak time is 40min, micro-inflation; Parent's emission index is 100%, and evenly, proterties is perfectly round, fertilization rate 97.4% for test under microscope ovum size.
Embodiment 4
On July 25th, 2013, sea, Leizhou prestige aquaculture Co., Ltd.Cultured population, shellfish 3 ages of age, the long 14.5-18.5cm of shell.Choose 8 individualities that select gonad maturity (male gonad milky, female sexual gland faint yellow), the quantity of male and female is respectively 5 and 3, the attachments such as the oyster that cleaning shellfish surface is adhered to, barnacle, lime worm.Utilize 0.02% disinfecting solution of potassium permanganate shellfish body 2min; Weighing 0.50g5-hydroxytryptamine, be dissolved in 10L sand filtration seawater (salinity 30.2ppt), the serotonin solution of compound concentration 0.05%; The parent is put into to the solution of preparation, soak time is 30min, micro-inflation; Parent's emission index is 100%, and evenly, proterties is perfectly round, fertilization rate 98.6% for test under microscope ovum size.
Embodiment 5
On August 18th, 2013, sea, Leizhou prestige aquaculture Co., Ltd.Cultured population, shellfish 3 ages of age, the long 15.5-17.9cm of shell.Choose 6 individualities that select gonad maturity (male gonad milky, female sexual gland faint yellow), the quantity of male and female is respectively 4 and 2, the attachments such as the oyster that cleaning shellfish surface is adhered to, barnacle, lime worm.Utilize 0.02% disinfecting solution of potassium permanganate shellfish body 1min; Weighing 0.3g5-hydroxytryptamine, be dissolved in 10L sand filtration seawater (salinity 29.5ppt), the serotonin solution of compound concentration 0.04%; The parent is put into to the solution of preparation, soak time is 40min, micro-inflation; Parent's emission index is 100%, and evenly, proterties is perfectly round, fertilization rate 97.8% for test under microscope ovum size.
Claims (6)
1. a method that improves large nacre parent gamete emission index, is characterized in that: comprise the steps:
(1) parent selects: select the long 14.0-20.0cm of shell from natural population or the cultured population in 3 ages or 4 ages, the individuality that vigor is strong, sexual gland is full is as the parent;
(2) parent's cleaning: parent transfer, to the indoor raising of seedling field, is cleared up to the attachment of shellfish surface, and the shellfish body is carried out disinfection; Utilize 0.02% disinfecting solution of potassium permanganate shellfish body;
(3) immersion treatment: the parent is soaked with serotonin solution.
2. require the method for described a kind of raising large nacre parent gamete emission index according to right 1, it is characterized in that: the described parent's sexual gland of step (1) is full.
3. require the method for described a kind of raising large nacre parent gamete emission index according to right 1, it is characterized in that: the described sterilization of step (2) refers to 0.02% disinfecting solution of potassium permanganate shellfish body 1-2min.
4. require the method for described a kind of raising large nacre parent gamete emission index according to right 1, it is characterized in that: the mass concentration of the described serotonin solution of step (3) is 0.02-0.05%.
5. require the method for described a kind of raising large nacre parent gamete emission index according to right 1, it is characterized in that: the soak time of the described immersion of step (3) is 30-50min.
6. require the method for described a kind of raising large nacre parent gamete emission index according to right 1, it is characterized in that: the compound method of the described serotonin solution of step (3) is: weighing 0.2-0.5g5-hydroxytryptamine, be dissolved in the 10L filtering sea, seawater salinity 28-30ppt.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106577413A (en) * | 2016-11-01 | 2017-04-26 | 海南大学 | Artificially induced spawning method of tridacnas |
| CN107173287A (en) * | 2016-07-03 | 2017-09-19 | 李育培 | A kind of induced spawning method of giant clam |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107173287A (en) * | 2016-07-03 | 2017-09-19 | 李育培 | A kind of induced spawning method of giant clam |
| CN106577413A (en) * | 2016-11-01 | 2017-04-26 | 海南大学 | Artificially induced spawning method of tridacnas |
| CN106577413B (en) * | 2016-11-01 | 2019-07-12 | 海南大学 | A kind of artificial induction's oviposition method of giant clam |
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