CN1736176A - Technique for producing sargassum thunbeergii kuntze offspring - Google Patents
Technique for producing sargassum thunbeergii kuntze offspring Download PDFInfo
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- CN1736176A CN1736176A CN 200510044618 CN200510044618A CN1736176A CN 1736176 A CN1736176 A CN 1736176A CN 200510044618 CN200510044618 CN 200510044618 CN 200510044618 A CN200510044618 A CN 200510044618A CN 1736176 A CN1736176 A CN 1736176A
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- thunbeergii
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Abstract
The invention discloses a seedling producing technique for rat-tailed algae, applying to seedling breed in-door according to sexual reproduction law, including: selecting and collecting the seed algae, stimulating and diffusing the seed algae, collecting the juvenile sporophyte, culturing and catadromousing seedlings, provided an artificial seedling producing technique for rat-tailed algae, dissolving the current shortcoming problem of the rat-tailed algae seedlings.
Description
One, technical field
The invention belongs to the algae culturing technical field, relate to artificial seed's production technology of a kind of large-scale economic algae-mouse tail algae.
Two, background technology
Mouse tail algae is a kind of important economic algae, present four aspects of its figures in the expression of value of the linen, as an equivalent, and consequently, as a thing that is value.(1) medicines and health protection: can extract agglutinin, antibiotic active substance and dextran; Its polysaccharose substance is often used as immunopotentiator, and the brown alga polyphenol is considered to the marine organisms natural; Mouse tail algae is dried to grind into powder and is used for thyromegaly, scrofula, anginal treatment.(2) chemical industry: can extract the bigger industrial chemicals of economic worth such as mannitol, iodine.(3) aquaculture: can be used as sea cucumber, haliotis discus hannai Ino, bay scallop is grown seedlings and the bait formed.(4) ecology: the ability of mouse tail algae enrichment inorganic arsenic is stronger, has the effect that purifies the sea area to a certain extent.
Mouse tail algae is nutritious, does not especially contain a large amount of phycocolloid, is irreplaceable high-quality bait in stichopus japonicus artificial breeding and the breed.In recent years, along with the development of apostichopus japonicus culture industry, the mouse tail algae of the self-sow of gathering has in a large number been caused the depletion of a lot of local suppliess.It is urgent day by day to explore mouse tail algae artificial breeding technique.
Mouse tail algae (Sargassum thunbergii) belongs to Phaeophyta, Fucales, Sargassaceae, Sargassum, dioecism.Modes of reproduction has two kinds under field conditions (factors): sexual propagation and nourishing and generating, based on nourishing and generating of holdfast regeneration plant, sexual propagation is auxilliary.The solution of Sargassum thunbergii offspring mainly contains three kinds: 1, utilizing the sexual reproduction rule to carry out indoor raising of seedling, is the main path that mouse tail algae breeding production seed is provided at present.2, tissue culture quick breeding is in the laboratory test stage at present, also has suitable distance from production application.3, utilizing the perennial characteristics of holdfast of mouse tail algae, gather natural resources, carry out many batches of breed, is the minor path that the breeding production seed is provided at present.
Stichopus japonicus is the emphasis kind of mariculture in recent years, and cultured area reaches 360,000 mu, can realize more than 70 hundred million yuan of the output values, occupies quite great proportion in the mariculture gross output value.Yet along with the fast development of sea cucumber large-scale farming, limited natural bait can not satisfy demand of practical production.Use that mouse tail algae (powder) grinds liquid or be that the Articial bait making of main component is thrown something and fed and become a kind of inexorable trend with mouse tail algae.At present, the market price that mouse tail algae fresh vegetable per kilogram is 4.0 yuan just fully shows mouse tail algae great demand and the wide industrialization prospect of carrying out seed breeding.
Three, summary of the invention
Purpose of the present invention mainly provides a kind of mouse tail algae artificial seed's production technology, effectively solves present mouse tail algae and cultures seed problem in short supply.
The object of the present invention is achieved like this, a kind of technique for producing sargassum thunbeergii kuntze offspring, be characterized in utilizing the sexual reproduction rule to carry out indoor seed breeding, comprise that the selection collection of kind of algae, the stimulation of planting algae are diffused, collection, the Seedling culturing device spatfall of juvenile sporophyte, cultivate and plunge into the commercial sea.
Plant the selection collection of algae, collect seedling when the highest water temperature reaches 20-23 ℃ in mouse tail algae vitellarium day, select more than the acquisition length 30cm, yellowish-brown has receptacle, grows stalwartness, and no receptacle comes off and point declines mashed frond slightly as kind of an algae.
Plant the stimulation of algae and diffuse, stimulating method has flowing water and dries in the shade two kinds, and diffusing is kind of an algae to be put into pond flowing water store up and diffuse, the amount of storing up 10-20kg/m
3, water temperature 22-25 ℃.
The collection of juvenile sporophyte after planting algae and diffusing 24 hours, will be planted algae and move into its pond and continue to store up and diffuse, and juvenile sporophyte water is leached impurity and mucus with 80 mesh sieve thin,tough silk earlier, filter small-sized impurity with 200 mesh sieve thin,tough silk then.Planting algae diffuses 2-3 time.
The Seedling culturing device spatfall is placed in the Seedling culturing device plane formula in the pond of filling water in advance, then the juvenile sporophyte water that concentrates is splashed equably to the pond water surface sprinkling amount 100-200 strain/cm
2, two days begin flowing water later on, during the spatfall water temperature 22-25 ℃, if water temperature is too high, keep water temperature with miniflow water.
Cultivation is that flowing water is cultivated in the pond, water temperature 15-22 ℃, adopts lamp, intensity of illumination 1000-20000lx, nutritive salt NO
3-N6-10g/m
3, PO
4-P1-2g/m
3, brushing method adopts the hydro-peening method, beginning in two days behind the spatfall, and weekly twice, it is ascending to scrub pressure, 0.5kg/cm
2-1.0kg/cm
2, clear pond 10-15 days once, upgrades water for larval nursing during clear pond.
Plunge into the commercial sea, indoor culture is the time about one month, plunges into the commercial sea about the general big or small 0.5cm of sporophyte and support temporarily.
The present invention utilizes the sexual reproduction rule of mouse tail algae, adopts that kind algae is indoor and stores up the attached holder of (flowing water) → zygote (or at the bottom of holder settling tank) to grow and be the technology path of juvenile sporophyte → makings juvenile sporophyte water → Seedling culturing device spatfall → cultivation, acquisition production seed.
The present invention is by selecting to collect seedling opportunity to obtain the more kind algae that can discharge smart ovum simultaneously; Impel kind of algae to concentrate the smart ovum of discharging by flowing water and dry in the shade stimulation, the short time obtains a large amount of juvenile sporophytes; By repeatedly diffusing, kind of an algae is fully used; By two kinds of mesh silk cover filterings and scrubbing, removed most assorted algae, mud scum, cladocera, protozoa etc., be beneficial to adhering to and growing of juvenile sporophyte; By seedling rearing room internal control light, control salt, temperature control, scrub, control measures such as clear pond, flowing water, make sporophyte fast, healthy growth, saved cost, improved output of seedling.Carry out Sargassum thunbergii offspring with this technology and breed, the unit of water body 10,000-100,000 strains/m of can emerging
2(area under cultivation 1-10 mu).Having tangible production application is worth.
Tissue culture quick breeding is in the laboratory test stage at present, also has suitable distance from production application.Gather natural resources, carries out many batches of breed, the seed of solution is limited, and is to be cost to sacrifice natural resources, though can solve local seed for the moment, the destruction natural resources is serious.
Four, specific embodiments
Embodiment, a kind of technique for producing sargassum thunbeergii kuntze offspring is to utilize the sexual reproduction rule to carry out indoor seed breeding, and kind algae ripe under the natural conditions is discharged the juvenile sporophyte that forms behind the smart ovum, on the matrix attached to manual manufacture, carries out indoor culture.Comprise that the selection collection of kind of algae, the stimulation of planting algae are diffused, collection, the Seedling culturing device spatfall of juvenile sporophyte, cultivate and plunge into the commercial sea; The selection collection of kind of algae is to gather when vitellarium day, the highest water temperature reached 20-23 ℃ in mouse tail algae, selects more than the frond length 30cm, and yellowish-brown has receptacle, grows healthy and strongly, and no receptacle comes off and taper declines mashed frond is as kind of an algae; Plant the stimulation of algae and diffuse, stimulating method has flowing water and dries in the shade two kinds, and stimulation is to diffuse for synchronous the concentrating of impelling reproductive cell, and diffusing is kind of an algae to be put into pond flowing water store up and diffuse, the amount of storing up 10-20kg/m
3, water temperature 22-25 ℃; The collection of juvenile sporophyte is after kind of an algae is diffused 24 hours, will plant its pond of algae immigration and continue to diffuse, and juvenile sporophyte water is leached impurity and mucus with 80 mesh sieve thin,tough silk earlier, filters small-sized impurity with 200 mesh sieve thin,tough silk then; The Seedling culturing device spatfall is in advance the Seedling culturing device plane formula to be placed in the pond of filling water, then the juvenile sporophyte water that concentrates is splashed equably to the pond water surface sprinkling amount 100-200 strain/cm
2, two days begin normal flowing water later on, during the spatfall water temperature 22-25 ℃, if water temperature is too high, keep water temperature with miniflow water; Cultivation is that flowing water is cultivated in the pond, water temperature 15-22 ℃, adopts lamp, intensity of illumination 1000-20000lx, nutritive salt NO
3-N6-10g/m
3, PO
4-P1-2g/m
3, brushing method adopts the hydro-peening method, beginning in two days behind the spatfall, and weekly twice, it is ascending to scrub pressure, 0.5kg/cm
2-1.0kg/cm
2, clear pond 10-15 days once, upgrades water for larval nursing during clear pond; Plunging into the commercial sea is indoor culture time about one month, plunges into the commercial sea about the general big or small 0.5cm of sporophyte to support temporarily to get final product.
Claims (7)
1, a kind of technique for producing sargassum thunbeergii kuntze offspring is characterized in that utilizing the sexual reproduction rule to carry out indoor seed breeding, comprises that the selection collection of kind of algae, the stimulation of planting algae are diffused, collection, the Seedling culturing device spatfall of juvenile sporophyte, cultivates and plunge into the commercial sea.
2, a kind of technique for producing sargassum thunbeergii kuntze offspring according to claim 1, it is characterized in that the selection collection of kind of algae, collect seedling when the highest water temperature reaches 20-23 ℃ in mouse tail algae vitellarium day, select more than the acquisition length 30cm, yellowish-brown, have receptacle, grow stalwartness, no receptacle comes off and point declines mashed frond slightly as kind of an algae.
3, a kind of technique for producing sargassum thunbeergii kuntze offspring according to claim 1 is characterized in that the stimulation of kind of algae is diffused, and stimulating method has flowing water and dries in the shade two kinds, and diffusing is kind of an algae to be put into pond flowing water store up and diffuse, the amount of storing up 10-20kg/m
3, water temperature 22-25 ℃.
4, a kind of technique for producing sargassum thunbeergii kuntze offspring according to claim 1, it is characterized in that the collection of juvenile sporophyte, after the kind algae is diffused 24 hours, to plant algae moves into its pond and continues to store up and diffuse, juvenile sporophyte water is leached impurity and mucus with 80 mesh sieve thin,tough silk earlier, filter small-sized impurity with 200 mesh sieve thin,tough silk then, plant algae and diffuse 2-3 time.
5, a kind of technique for producing sargassum thunbeergii kuntze offspring according to claim 1, it is characterized in that the Seedling culturing device spatfall, in advance the Seedling culturing device plane formula is placed in the pond of filling water, then the juvenile sporophyte water that concentrates is splashed equably to the pond water surface sprinkling amount 100-200 strain/cm
2, two days begin flowing water later on, during the spatfall water temperature 22-25 ℃, if water temperature is too high, keep water temperature with miniflow water.
6, a kind of technique for producing sargassum thunbeergii kuntze offspring according to claim 1, it is characterized in that cultivating is that flowing water is cultivated in the pond, water temperature 15-22 ℃, adopts lamp, intensity of illumination 1000-200001x, nutritive salt NO
3-N6-10g/m
3, PO
4-P 1-2g/m
3, brushing method adopts the hydro-peening method, beginning in two days behind the spatfall, and weekly twice, it is ascending to scrub pressure, 0.5kg/cm
2-1.0kg/cm
2, clear pond 10-15 days once, upgrades water for larval nursing during clear pond.
7, a kind of technique for producing sargassum thunbeergii kuntze offspring according to claim 1 is characterized in that plunging into the commercial sea, and indoor culture is the time about one month, plunges into the commercial sea about the general big or small 0.5cm of sporophyte and supports temporarily.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101263780B (en) * | 2008-04-24 | 2010-06-09 | 山东省海水养殖研究所 | Method for breeding Sargassum thunbergii large size seedling in northern sea area ahead of time |
| CN1943318B (en) * | 2006-10-26 | 2011-06-15 | 威海西港水产刺参原种场有限公司 | Full artificial seedling nursing technology for sargassum thunbergii |
| CN103004576A (en) * | 2012-12-20 | 2013-04-03 | 山东省海水养殖研究所 | Method for artificially cultivating Sargassum thunbergii in intertidal zones |
| CN103039350A (en) * | 2013-01-22 | 2013-04-17 | 中国水产科学研究院黄海水产研究所 | Seedling cultivating method for improving emergence rate and growth rate of sargassum thunbergii |
| CN103444505A (en) * | 2013-09-05 | 2013-12-18 | 山东省海水养殖研究所 | Method for propagating sargassum thunbergii in intertidal zones |
| CN103477805A (en) * | 2013-09-03 | 2014-01-01 | 浙江省海洋水产养殖研究所 | Sargassum horneri spore collecting device and use method thereof |
| CN105993907A (en) * | 2016-06-21 | 2016-10-12 | 山东省海洋生物研究院 | Seedling collecting method and early breeding method of sargassum thunbergii |
| CN109729968A (en) * | 2019-03-19 | 2019-05-10 | 山东省海洋生物研究院 | The proliferation big specification breeding method of sargassum thunbergii seedling in a kind of sea jungle nursery |
| CN113980884A (en) * | 2021-11-15 | 2022-01-28 | 烟台大学 | Cell line purification method for hemerocallis fulva cell engineering seedling breeding |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1631119A (en) * | 2004-12-24 | 2005-06-29 | 中国海洋大学 | Sargassum thunbergii offspring breeding method based on somatocyte seeding raising technology |
-
2005
- 2005-09-01 CN CNB2005100446189A patent/CN100376668C/en not_active Expired - Fee Related
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1943318B (en) * | 2006-10-26 | 2011-06-15 | 威海西港水产刺参原种场有限公司 | Full artificial seedling nursing technology for sargassum thunbergii |
| CN101263780B (en) * | 2008-04-24 | 2010-06-09 | 山东省海水养殖研究所 | Method for breeding Sargassum thunbergii large size seedling in northern sea area ahead of time |
| CN103004576A (en) * | 2012-12-20 | 2013-04-03 | 山东省海水养殖研究所 | Method for artificially cultivating Sargassum thunbergii in intertidal zones |
| CN103039350A (en) * | 2013-01-22 | 2013-04-17 | 中国水产科学研究院黄海水产研究所 | Seedling cultivating method for improving emergence rate and growth rate of sargassum thunbergii |
| CN103039350B (en) * | 2013-01-22 | 2014-01-08 | 中国水产科学研究院黄海水产研究所 | Seedling cultivating method for improving emergence rate and growth rate of sargassum thunbergii |
| CN103477805A (en) * | 2013-09-03 | 2014-01-01 | 浙江省海洋水产养殖研究所 | Sargassum horneri spore collecting device and use method thereof |
| CN103444505A (en) * | 2013-09-05 | 2013-12-18 | 山东省海水养殖研究所 | Method for propagating sargassum thunbergii in intertidal zones |
| CN103444505B (en) * | 2013-09-05 | 2015-12-02 | 山东省海水养殖研究所 | The method of a kind of sargassum thunbergii intertidal zone propagation |
| CN105993907A (en) * | 2016-06-21 | 2016-10-12 | 山东省海洋生物研究院 | Seedling collecting method and early breeding method of sargassum thunbergii |
| CN109729968A (en) * | 2019-03-19 | 2019-05-10 | 山东省海洋生物研究院 | The proliferation big specification breeding method of sargassum thunbergii seedling in a kind of sea jungle nursery |
| CN113980884A (en) * | 2021-11-15 | 2022-01-28 | 烟台大学 | Cell line purification method for hemerocallis fulva cell engineering seedling breeding |
| CN113980884B (en) * | 2021-11-15 | 2023-11-17 | 烟台大学 | Cell line purification method for breeding hemerocallis cell engineering seedlings |
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| CN100376668C (en) | 2008-03-26 |
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