CN103444505A - Method for propagating sargassum thunbergii in intertidal zones - Google Patents
Method for propagating sargassum thunbergii in intertidal zones Download PDFInfo
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- CN103444505A CN103444505A CN2013104013684A CN201310401368A CN103444505A CN 103444505 A CN103444505 A CN 103444505A CN 2013104013684 A CN2013104013684 A CN 2013104013684A CN 201310401368 A CN201310401368 A CN 201310401368A CN 103444505 A CN103444505 A CN 103444505A
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- adherance
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
Abstract
The invention relates to a method for propagating sargassum thunbergii in intertidal zones. Aimed at the current situation that the sargassum thunbergii resource severely declines and that the marine ecology is destroyed, the method screens out an intertidal zone environment suitable for the survival and growth of sargassum thunbergii, and propagates sargassum thunbergii with sexually propagated juvenile sporophytes of sargassum thunbergii as a seed source by utilizing circular arc-shaped artificial brick substrates and using accelerated cement to fix the seed-attached substrates on pure natural reefs in order to restore the natural sargassum thunbergii resource, protect the coastal ecological environment and provide basic material for the rehabilitation of the marine ecology; an artificial sargassum thunbergii farm built by the method can propagate the population from generation to generation, and the method can provide technical support for the building of large alga farms and the rehabilitation of marine ecology.
Description
Technical field
The invention belongs to marine alga multiplication technique field, be specifically related to the method for a kind of sargassum thunbergii intertidal zone propagation.
Background technology
Sargassum thunbergii (Sargassum thunbergii) is a kind of in Phaeophyta (Phaeophyta), Fucales (Fucales), Sargassaceae (Sargassaceae), Sargassum (Sargassum), China north is to Liaodong Peninsula, reaching the Lezhou Peninsula in the south all has distribution, is a kind of kelp of intertidal zone.In recent years, the economy of sargassum thunbergii and the ecological value have caused people's attention, sargassum thunbergii is the high-quality feed of sea cucumber seedlings on the one hand, fast development due to the holothruian cultures industry, wild resource can not satisfy the demands far away, be to be the tangleweed of intertidal zone growth due to sargassum thunbergii on the other hand, and often become sociales, its biomass can be up to 5kg/m
2above, in the environmental ecology reparation of offshore waters, play an important role.Therefore, carry out the sargassum thunbergii stock enhancement imperative.
Summary of the invention
The present invention is directed to above requirement, invented a kind of method of sargassum thunbergii artificial fecundation, to recovering the sargassum thunbergii resource, and further increase the biomass of sargassum thunbergii.
Sargassum thunbergii artificial fecundation method of the present invention comprises following step:
1) preparation of adherance: make the adherance that is shaped as circular arc with concrete material, at the outer surface of adherance, be attached with little fragment of brick;
2) set of sargassum thunbergii juvenile sporophyte: by adherance indoor after immersion treatment, put into the nursery pond hydrostatic and adopt attached sargassum thunbergii juvenile sporophyte, indoor semi-fluid water planting is educated 15-20 days;
3) selection of propagation environment: select the fixed location of the pocket of intertidal zone as adherance, pocket is blocked to leakage point with accelerated cement, form artificial dam, in the dykes and dams bottom, add draining hole;
4) adherance is fixing: during the long 2mm of juvenile sporophyte average body on the adherance of indoor raising of seedling, in the time of low water, adherance is fixed in to selected fixed location with accelerated cement: at first the seawater in set place is emitted by draining hole, then with hairbrush, carry out reef clearly; To be transported to the propagation place from nursery with the adherance of juvenile sporophyte, with accelerated cement, adherance will be fixed on the reef through clear reef; Wait for eagre, do not have adherance, block draining hole;
5) cultivation of sargassum thunbergii: after adherance is fixed on to appointed area, regularly observed its growing state in breeding process, remove aufwuch and algophagous aminal; After firmly growing on artificial adherance, propagation sargassum thunbergii rhizoid proceeds to low level management.
The arc diameter 18-25cm of described adherance, high 2-5cm in the middle of it;
The wet cloth of adherance when transportation surface coverage in described step 4), prevent from that temperature of exposure and dry is long seedling is caused damage;
In described step 4), if the artificial seedling on adherance is less, in spring season, when stormy waves is less, the sargassum thunbergii spore fluid of splashing, in the adherance surface, is carried out and is manually filled the gaps with seedlings.
This method is that artificial breeding and the combination of marine alga multiplication technique are carried out to sargassum thunbergii propagation, and adopt the sargassum thunbergii artificial breeding early stage, can make seed density increase, later stage is selected suitable propagation place, take original habitat as basis, and appropriate reconstruction, can successfully realize the purpose that sargassum thunbergii is bred.Result shows, at original sargassum thunbergii, less zone or the zone do not had, as long as through appropriate reconstruction, can realize the proliferation of high-density of sargassum thunbergii, increases its biomass.There are following two major advantages: the one, easy and simple to handle, cost is lower, easily promotes, and in suitable place, can adopt this method to carry out sargassum thunbergii propagation.The 2nd, cultivation effect is obvious, and biomass is significantly increased.
Embodiment
Below in conjunction with embodiment, method of the present invention is described in detail.
Embodiment 1
1) preparation of adherance: make the adherance that is shaped as circular arc with concrete material, at the outer surface of adherance, be attached with little fragment of brick;
The preparation method of adherance is: use diameter 22cm, the circular arc plastic tube of high 2cm is made mould, loads concrete, then at the concrete upper surface, adheres to little fragment of brick.
Adherance volume used in the present invention is little, is applicable to the environment that adheres to of intertidal zone complexity; Upper surface adheres to fragment of brick, utilizes the characteristic of the rough porous on fragment of brick surface, sargassum thunbergii seedling adhesion-tight, wind wave resistance.
2) set of sargassum thunbergii juvenile sporophyte: by adherance indoor after immersion treatment, put into nursery pond and adopt hydrostatic to adopt attached sargassum thunbergii juvenile sporophyte, indoor semi-fluid water planting is educated 15 days;
Detailed process is as follows: collect the frond in maturing stage, be put in indoor large pond, allow spore naturally come off, then frond and reproduction branch are taken out.After adhering to 48 hours, every day is changed seawater once in large pond, with water pump, water is constantly circulated at ordinary times, and first 10 days, water body day exchange 4 times, exchange in 10 days thereafter 7 times, the NO of interpolation 4:0.4ppm in every cubic metre of seawater
3-N and PO
4-P, guarantee its required nutrition of growing, and it is 22 ± 1.5 ℃ that illumination adopts Indoor Natural scattered light temperature, and the adherance of growing seedlings is used above-mentioned adherance.About 20 days, seed grows to 0.2-0.4cm and can be used for propagation.This seedling raising process should be noted that one is to strengthen quantity of exchanged water and use water pump to circulate, and purpose is to make sargassum thunbergii seedling firm attachment on adherance, and the morning and evening tides that adapts to intertidal zone impacts; The 2nd, add nutritive salt, promote the sargassum thunbergii growth of health fast, guarantee that seed is sturdy.
3) selection of propagation environment: select the fixed location of the pocket in intertidal zone climax band, middle tide band and low tide band propagation place as adherance, pocket is blocked to leakage point with accelerated cement, form artificial dam, at dykes and dams, near the direction of low tide band, add draining hole;
Add the purpose of draining hole to be that needs fixedly during adherance, can open draining hole, seawater is drained off, wait to have fixed, block draining hole, at seawater at the incoming tide, can not have adherance, avoid seedling to receive exposure in sunshine, and seawater can provide the nutritive element of seedling Fast Growth.
4) adherance is fixing: during the long 2mm of juvenile sporophyte average body on adherance, in the time of low water, adherance is fixed in to selected fixed location with accelerated cement: at first the seawater in set place is emitted by draining hole, then with hairbrush, carry out reef clearly; To be transported to the propagation place from nursery with the adherance of juvenile sporophyte, with accelerated cement, adherance will be fixed on the reef through clear reef; Wait for eagre, do not have adherance, block draining hole;
The marine site, intertidal zone that the cultivation place of selecting in the present embodiment is valgar island, Li Dao town, Rongcheng, Shandong ocean science limited company, implement 500 square metres of areas.
5) cultivation of sargassum thunbergii: after adherance is fixed on to appointed area, regularly observed its growing state in breeding, remove aufwuch and weed eater; When propagation sargassum thunbergii rhizoid firmly grows on artificial adherance, length proceeds to low level management after 0.5cm.
For culture efficiency more of the present invention, in identical marine site, similarly under topographic features, according to existing method, carry out the cultivation of sargassum thunbergii simultaneously.Main implementation methods different from existing method are: one, and conventional shell is selected on the adherance surface; Two, do not use nutritive salt between the sargassum thunbergii nursery stage; Three, the propagation environment does not adopt artificial dam, will have the adherance of seedling directly to be fixed on exposed reef.Found that, cultivation effect is not remarkable, and reason is that one is that shell surface is smooth, and the sargassum thunbergii seedling can not firmly be adsorbed on the adherance surface; The 2nd,, if nonnutritive salt, the Sargassum thunbergii offspring poor growth, miss its optimum growh season, when artificial fecundation, the seed growth is slow, can not form the population advantage, and the impacts such as assorted algae are arranged; The 3rd,, the sargassum thunbergii seedling of propagation is subject to direct sunlight, and losing time is long, and lethality is very high.
Embodiment 2
1) preparation of adherance: make the adherance that is shaped as circular arc with concrete material, at the outer surface of adherance, be attached with little fragment of brick;
The preparation method of adherance is: use diameter 25cm, the plastic tube of high 2cm is made mould, loads concrete, then at the concrete upper surface, adheres to little fragment of brick.
2) set of sargassum thunbergii juvenile sporophyte: by adherance indoor after immersion treatment, put into nursery pond and adopt hydrostatic to adopt attached sargassum thunbergii juvenile sporophyte, indoor semi-fluid water planting is educated 20 days;
Sargassum thunbergii seedling raising process: collect the frond in maturing stage, be put in indoor large pond, allow it naturally come off, then frond and reproduction branch are taken out.After adhering to 48 hours, every day is changed seawater once in large pond, with water pump, water is constantly circulated at ordinary times, and first 10 days, water body day exchange 6 times, exchange in 10 days thereafter 9 times, the NO of interpolation 4:0.4ppm in every cubic metre of seawater
3-N and PO
4-P, guarantee its required nutrition of growing, and it is 22 ± 1.5 ℃ that illumination adopts Indoor Natural scattered light temperature, and the adherance of growing seedlings is used above-mentioned adherance.About 20 days, seed grows to 0.2-0.4cm and can be used for propagation.This seedling raising process should be noted that one is to strengthen quantity of exchanged water and use water pump to circulate, and purpose is to make sargassum thunbergii seedling firm attachment on adherance, and the morning and evening tides that adapts to intertidal zone impacts; The 2nd, add nutritive salt, promote the sargassum thunbergii growth of health fast, guarantee that seed is sturdy.
3) selection of propagation environment: select that in intertidal zone, tide band and low tide band are as main place, pocket, as the fixed location of adherance, is blocked leakage point by pocket with accelerated cement, forms artificial dam, in the direction of the close low tide band of dykes and dams, adds draining hole;
Add the purpose of draining hole to be that needs fixedly during adherance, can open draining hole, seawater is drained off, wait to have fixed, block draining hole, at seawater at the incoming tide, can not have adherance, avoid seedling to receive exposure in sunshine, and seawater can provide the nutritive element of seedling Fast Growth.
4) adherance is fixing: during the long 2mm of juvenile sporophyte average body on adherance, in the time of low water, adherance is fixed in to marine site, intertidal zone, Sang Gou gulf, Rongcheng, Shandong with accelerated cement, implement 450 square metres of areas: at first the seawater in set place is emitted by draining hole, then with hairbrush, carry out reef clearly; To be transported to the propagation place from nursery with the adherance of juvenile sporophyte, with accelerated cement, adherance will be fixed on the reef through clear reef; Wait for eagre, do not have adherance, block draining hole;
5) cultivation of sargassum thunbergii: after adherance is fixed on to appointed area, regularly observed its growing state in breeding, remove aufwuch and weed eater; When propagation sargassum thunbergii rhizoid firmly grows on artificial adherance, length proceeds to low level management after 0.5cm.
Cultivation effect by embodiment 1 and embodiment 2 shows, by the adult weight in wet base mean value computation of every square metre, method of the present invention is compared with conventional cultural method, in the output of low tide band, can be increased to 2.4kg by 1.5kg, increases by 60% biomass; Be increased to 2kg at middle tide band from 0.8kg, increase by 150%; Can be increased to 0.7kg from 0.2 at the climax band, be 2.5 times.And the result of large-scale farming is also close with the effect of above-described embodiment, thereby show that method of the present invention has good popularizing application prospect.
Claims (6)
1. a sargassum thunbergii artificial fecundation method comprises following step:
1) preparation of adherance: make the adherance that is shaped as circular arc with concrete material, at the outer surface of adherance, be attached with little fragment of brick;
2) set of sargassum thunbergii juvenile sporophyte: by adherance indoor after immersion treatment, put into the nursery pond hydrostatic and adopt attached sargassum thunbergii juvenile sporophyte, indoor semi-fluid water planting is educated 15-20 days;
3) selection of propagation environment: select the fixed location of the pocket of intertidal zone as adherance, pocket is blocked to leakage point with accelerated cement, form artificial dam, in the dykes and dams bottom, add draining hole;
4) adherance is fixing: during the long 2mm of juvenile sporophyte average body on the adherance of indoor raising of seedling, in the time of low water, adherance is fixed in to selected fixed location with accelerated cement: at first the seawater in set place is emitted by draining hole, then with hairbrush, carry out reef clearly; To be transported to the propagation place from nursery with the adherance of juvenile sporophyte, with accelerated cement, adherance will be fixed on the reef through clear reef; Wait for eagre, do not have adherance, block draining hole;
5) cultivation of sargassum thunbergii: after adherance is fixed on to appointed area, regularly observed its growing state in breeding process, remove aufwuch and algophagous aminal; After firmly growing on artificial adherance, propagation sargassum thunbergii rhizoid proceeds to low level management.
2. sargassum thunbergii artificial fecundation method as claimed in claim 1, is characterized in that the arc diameter 18-25cm of described adherance, high 2-5cm in the middle of it.
3. sargassum thunbergii artificial fecundation method as claimed in claim 1 or 2, is characterized in that the preparation method of described adherance, is to make mould with the circular arc plastic tube, loads concrete, then at the concrete upper surface, adheres to little fragment of brick and complete preparation.
4. sargassum thunbergii artificial fecundation method as claimed in claim 1, it is characterized in that described 2) in adopt attached sargassum thunbergii juvenile sporophyte, its method is as follows: at first collect the frond in maturing stage, be put in indoor large pond, allow spore naturally come off, then frond and reproduction branch are taken out; After adhering to 48 hours, every day is changed seawater once in large pond, with water pump, water is constantly circulated at ordinary times, and first 10 days, water body day exchange 4-6 time, exchanged 5-7 time in 10 days thereafter, adds respectively 4 in every cubic metre of seawater, the NO of 0.4ppm
3-N and PO
4-P, illumination adopts the Indoor Natural scattered light, and temperature is 22 ± 1.5 ℃.
5. sargassum thunbergii artificial fecundation method as claimed in claim 1, the wet cloth of surface coverage while it is characterized in that in described step 4) the adherance transportation.
6. sargassum thunbergii artificial fecundation method as claimed in claim 1, is characterized in that in described step 4), if the artificial seedling on adherance is less, in spring season, when stormy waves is less, the sargassum thunbergii spore fluid of splashing, in the adherance surface, is carried out and manually filled the gaps with seedlings.
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Cited By (8)
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| CN104488690A (en) * | 2014-12-26 | 2015-04-08 | 中国科学院海洋研究所 | Gracilaria seaweed algal turf repairing method |
| CN104521732A (en) * | 2015-01-30 | 2015-04-22 | 中国科学院海洋研究所 | Ecological restoration method of gracilaria algae |
| CN104839006A (en) * | 2015-06-02 | 2015-08-19 | 山东省海洋生物研究院 | Cofferdam multiplication method of sargassum thunbergii |
| CN106718814A (en) * | 2016-11-30 | 2017-05-31 | 山东省海洋生物研究院 | A kind of sargassum thunbergii seedling is collected seedling with attachment base and preparation method thereof |
| CN106718813A (en) * | 2016-11-30 | 2017-05-31 | 山东省海洋生物研究院 | A kind of slow-release fertilizer nutritional preparation suitable for the new attachment base of collecting seedling of sargassum thunbergii |
| CN109566395A (en) * | 2019-01-15 | 2019-04-05 | 浙江省海洋水产养殖研究所 | Littoral zone ecological system degradation method |
| CN109729968A (en) * | 2019-03-19 | 2019-05-10 | 山东省海洋生物研究院 | The proliferation big specification breeding method of sargassum thunbergii seedling in a kind of sea jungle nursery |
| CN110199867A (en) * | 2019-07-19 | 2019-09-06 | 山东省海洋生物研究院 | A kind of sargassum thunbergii proliferation algal reef system and its application method |
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| CN104488690A (en) * | 2014-12-26 | 2015-04-08 | 中国科学院海洋研究所 | Gracilaria seaweed algal turf repairing method |
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| CN106718814A (en) * | 2016-11-30 | 2017-05-31 | 山东省海洋生物研究院 | A kind of sargassum thunbergii seedling is collected seedling with attachment base and preparation method thereof |
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| CN106718814B (en) * | 2016-11-30 | 2019-11-08 | 山东省海洋生物研究院 | A kind of sargassum thunbergii seedling is collected seedling with attachment base and preparation method thereof |
| CN106718813B (en) * | 2016-11-30 | 2020-05-01 | 山东省海洋生物研究院 | Slow-release fertilizer nutrition preparation suitable for sargassum thunbergii seedling collection attachment base |
| CN109566395A (en) * | 2019-01-15 | 2019-04-05 | 浙江省海洋水产养殖研究所 | Littoral zone ecological system degradation method |
| CN109729968A (en) * | 2019-03-19 | 2019-05-10 | 山东省海洋生物研究院 | The proliferation big specification breeding method of sargassum thunbergii seedling in a kind of sea jungle nursery |
| CN110199867A (en) * | 2019-07-19 | 2019-09-06 | 山东省海洋生物研究院 | A kind of sargassum thunbergii proliferation algal reef system and its application method |
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