CN103444505B - The method of a kind of sargassum thunbergii intertidal zone propagation - Google Patents
The method of a kind of sargassum thunbergii intertidal zone propagation Download PDFInfo
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- CN103444505B CN103444505B CN201310401368.4A CN201310401368A CN103444505B CN 103444505 B CN103444505 B CN 103444505B CN 201310401368 A CN201310401368 A CN 201310401368A CN 103444505 B CN103444505 B CN 103444505B
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- adherance
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
Abstract
The present invention relates to a kind of sargassum thunbergii enrichment procedure, the present situation that the method is destroyed for the slump of disastrous proportions of current sargassum thunbergii resource and marine ecology, screening is suitable for the intertidal zone environment of sargassum thunbergii survival and growth, utilize the artificial fragment of brick adherance of circular arc, with the sargassum thunbergii juvenile sporophyte of sexual propagation for seedling source, the attachment after spatfall is fixed based on ecosystem nature reef with accelerated cement, carry out the propagation of sargassum thunbergii, to recovering natural sargassum thunbergii resource, protect coastal ecotope, for marine ecology reparation provides basic data; Population can be multiplied from generation to generation in the artificial sargassum thunbergii field that this method builds, and can be to build tangleweed field and marine ecology reparation and provide technical support.
Description
Technical field
The invention belongs to marine alga multiplication technique field, be specifically related to the method for a kind of sargassum thunbergii intertidal zone propagation.
Background technology
Sargassum thunbergii (Sargassumthunbergii) is the one in Phaeophyta (Phaeophyta), Fucales (Fucales), Sargassaceae (Sargassaceae), Sargassum (Sargassum), China north is to Liaodong Peninsula, reaching the Lezhou Peninsula in the south and all have distribution, is a kind of kelp of intertidal zone.In recent years, the economy of sargassum thunbergii and the ecological value cause the attention of people, sargassum thunbergii is the high-quality feed of sea cucumber seedlings on the one hand, due to the fast development of holothruian cultures industry, wild resource can not satisfy the demands far away, be because sargassum thunbergii is the tangleweed that intertidal zone grows on the other hand, and often become sociales, its biomass can up to 5kg/m
2above, play an important role in the environmental ecology reparation of offshore waters.Therefore, sargassum thunbergii stock enhancement is carried out imperative.
Summary of the invention
The present invention is directed to above requirement, invented a kind of method of sargassum thunbergii artificial fecundation, to recovering sargassum thunbergii resource, and increasing the biomass of sargassum thunbergii further.
Sargassum thunbergii artificial fecundation method of the present invention, comprises following step:
1) preparation of adherance: make with concrete material the adherance that shape is circular arc, be attached with little fragment of brick at the outer surface of adherance;
2) set of sargassum thunbergii juvenile sporophyte: by adherance in indoor after immersion treatment, put into nursery pond hydrostatic and adopt attached sargassum thunbergii juvenile sporophyte, indoor half running water technology 15-20 days;
3) selection of environment is bred: select the pocket of intertidal zone as the fixed location of adherance, pocket accelerated cement is blocked leakage point, form artificial dam, add draining hole in dykes and dams bottom;
4) adherance is fixing: during the long 2mm of the juvenile sporophyte average body on the adherance of indoor raising of seedling, in the time of low water, adherance is fixed on selected fixed location with accelerated cement: first released by draining hole by the seawater in set place, then carries out reef clearly with hairbrush; Adherance with juvenile sporophyte is transported to propagation place from nursery, with accelerated cement, adherance is fixed on the reef of clear reef; Wait for eagre, do not have adherance, and blocked draining hole;
5) cultivation of sargassum thunbergii: after adherance is fixed on appointed area, regularly carries out observing its growing state in breeding process, removes aufwuch and algophagous aminal; Low level management is proceeded to when propagation sargassum thunbergii rhizoid is firmly grown on after on artificial adherance.
The arc diameter 18-25cm of described adherance, high 2-5cm in the middle of it;
In described step 4), during adherance transport, surface coverage wets cloth, prevents that temperature of exposure and dry is long to cause damage to seedling;
In described step 4), if the hatchery juveniles on adherance is less, in spring season, time stormy waves is less, sargassum thunbergii spore fluid of splashing, in adherance surface, is carried out and is manually filled the gaps with seedlings.
This method artificial breeding and marine alga multiplication technique is combined to carry out sargassum thunbergii propagation, and early stage adopts sargassum thunbergii artificial breeding, and seed density can be made to increase, later stage selects suitable propagation place, based on original habitat, appropriate reconstruction, successfully can realize the object of sargassum thunbergii propagation.Result shows, in the less region of original sargassum thunbergii or the region that do not have, as long as through appropriate reconstruction, can realize the proliferation of high-density of sargassum thunbergii, increase its biomass.There are following two major advantages: one is easy and simple to handle, and cost is lower, easily promotes, this method can be adopted to carry out sargassum thunbergii propagation in suitable place.Two is that cultivation effect is obvious, and biomass is significantly increased.
Embodiment
Below in conjunction with embodiment, method of the present invention is described in detail.
Embodiment 1
1) preparation of adherance: make with concrete material the adherance that shape is circular arc, be attached with little fragment of brick at the outer surface of adherance;
The preparation method of adherance is: use diameter 22cm, and the circular arc plastic tube of high 2cm makees mould, loads concrete, then adheres to little fragment of brick at concrete upper surface.
Adherance volume used in the present invention is little, is applicable to the attachment environment of intertidal zone complexity; Upper surface adheres to fragment of brick, utilizes the characteristic of the rough porous on fragment of brick surface, sargassum thunbergii seedling adhesion-tight, wind wave resistance.
2) set of sargassum thunbergii juvenile sporophyte: by adherance in indoor after immersion treatment, put into nursery pond and adopt hydrostatic to adopt attached sargassum thunbergii juvenile sporophyte, indoor half running water technology 15 days;
Detailed process is as follows: the frond collecting the maturing stage, is put in indoor large pond, allows spore naturally come off, then frond and fertile tillers are taken out.Adhere to after 48 hours, every day is changed seawater once in large pond, with water pump, water is constantly circulated at ordinary times, and first 10 days, water body day exchanged 4 times, within 10 days thereafter, exchanges 7 times, adds the NO of 4:0.4ppm in every cubic metre of seawater
3-N and PO
4-P, ensure its nutrition needed for growth, illumination adopts Indoor Natural scattered light temperature to be 22 ± 1.5 DEG C, and nursery adherance uses above-mentioned adherance.About 20 days, seed grew to 0.2-0.4cm and namely can be used for propagation.This seedling raising process should be noted that one is strengthen quantity of exchanged water and use water pump to circulate, and object is to make sargassum thunbergii seedling be firmly attached on adherance, and the tide adapting to intertidal zone impacts; Two is add nutritive salt, promotes the growth that sargassum thunbergii is healthy fast, ensures that seed is sturdy.
3) selection of environment is bred: select intertidal zone climax band, middle tide band and low tide band to breed the fixed location of pocket as adherance in place, pocket accelerated cement is blocked leakage point, form artificial dam, add draining hole at dykes and dams near the direction of low tide band;
When the object adding draining hole is to need fixing adherance, draining hole can be opened, seawater is drained off, to be fixed complete, block draining hole, can adherance do not had at seawater at the incoming tide, seedling is avoided to receive exposure in sunshine, and the nutritive element that seawater can provide seedling to grow fast.
4) adherance is fixing: as the long 2mm of the juvenile sporophyte average body on adherance, in the time of low water, adherance is fixed on selected fixed location: first released by draining hole by the seawater in set place, then carries out reef clearly with hairbrush with accelerated cement; Adherance with juvenile sporophyte is transported to propagation place from nursery, with accelerated cement, adherance is fixed on the reef of clear reef; Wait for eagre, do not have adherance, and blocked draining hole;
The cultivation place selected in the present embodiment is the marine site, intertidal zone of Li Daozhenli island, Rongcheng, Shandong ocean science limited company, implements area 500 square metres.
5) cultivation of sargassum thunbergii: after adherance is fixed on appointed area, regularly carries out observing its growing state in breeding, removes aufwuch and weed eater; When propagation sargassum thunbergii rhizoid is firmly grown on artificial adherance, length proceeds to low level management after 0.5cm.
Simultaneously in order to culture efficiency more of the present invention, in identical marine site, under similar topographic features, carry out the cultivation of sargassum thunbergii according to existing method.Main implementation method different from existing method is: one, and conventional shell is selected on adherance surface; Two, do not use nutritive salt between sargassum thunbergii nursery stage; Three, propagation environment does not adopt artificial dam, is directly fixed on there being the adherance of seedling on exposed reef.Found that, cultivation effect is not remarkable, and reason is that one is that shell surface is smooth, and sargassum thunbergii seedling can not firmly be adsorbed on adherance surface; Two are, if nonnutritive salt, Sargassum thunbergii offspring poor growth, misses its optimum growh season, and when artificial fecundation, seed growth is slow, can not form population advantage, has the impacts such as assorted algae; Three are, the sargassum thunbergii seedling of propagation is subject to direct sunlight, and losing time is long, and lethality is very high.
Embodiment 2
1) preparation of adherance: make with concrete material the adherance that shape is circular arc, be attached with little fragment of brick at the outer surface of adherance;
The preparation method of adherance is: use diameter 25cm, and the plastic tube of high 2cm makees mould, loads concrete, then adheres to little fragment of brick at concrete upper surface.
2) set of sargassum thunbergii juvenile sporophyte: by adherance in indoor after immersion treatment, put into nursery pond and adopt hydrostatic to adopt attached sargassum thunbergii juvenile sporophyte, indoor half running water technology 20 days;
Sargassum thunbergii seedling raising process: the frond collecting the maturing stage, is put in indoor large pond, allows it naturally come off, then frond and fertile tillers are taken out.Adhere to after 48 hours, every day is changed seawater once in large pond, with water pump, water is constantly circulated at ordinary times, and first 10 days, water body day exchanged 6 times, within 10 days thereafter, exchanges 9 times, adds the NO of 4:0.4ppm in every cubic metre of seawater
3-N and PO
4-P, ensure its nutrition needed for growth, illumination adopts Indoor Natural scattered light temperature to be 22 ± 1.5 DEG C, and nursery adherance uses above-mentioned adherance.About 20 days, seed grew to 0.2-0.4cm and namely can be used for propagation.This seedling raising process should be noted that one is strengthen quantity of exchanged water and use water pump to circulate, and object is to make sargassum thunbergii seedling be firmly attached on adherance, and the tide adapting to intertidal zone impacts; Two is add nutritive salt, promotes the growth that sargassum thunbergii is healthy fast, ensures that seed is sturdy.
3) selection of environment is bred: in selection intertidal zone, tide band and low tide band are as main place, and pocket accelerated cement, as the fixed location of adherance, is blocked leakage point by pocket, form artificial dam, add draining hole at dykes and dams near the direction of low tide band;
When the object adding draining hole is to need fixing adherance, draining hole can be opened, seawater is drained off, to be fixed complete, block draining hole, can adherance do not had at seawater at the incoming tide, seedling is avoided to receive exposure in sunshine, and the nutritive element that seawater can provide seedling to grow fast.
4) adherance is fixing: as the long 2mm of the juvenile sporophyte average body on adherance, in the time of low water, adherance is fixed on marine site, intertidal zone, Sang Gou gulf, Rongcheng, Shandong with accelerated cement, implement area 450 square metres: first released by draining hole by the seawater in set place, then carry out reef clearly with hairbrush; Adherance with juvenile sporophyte is transported to propagation place from nursery, with accelerated cement, adherance is fixed on the reef of clear reef; Wait for eagre, do not have adherance, and blocked draining hole;
5) cultivation of sargassum thunbergii: after adherance is fixed on appointed area, regularly carries out observing its growing state in breeding, removes aufwuch and weed eater; When propagation sargassum thunbergii rhizoid is firmly grown on artificial adherance, length proceeds to low level management after 0.5cm.
Shown by the cultivation effect of embodiment 1 and embodiment 2, by the adult weight in wet base mean value computation of every square metre, method of the present invention, compared with conventional cultural method, can be increased to 2.4kg by 1.5kg in the output of low tide band, increases by the biomass of 60%; Be increased to 2kg at middle tide band from 0.8kg, increase by 150%; Can be increased to 0.7kg from 0.2 at climax band, be 2.5 times.And the result of large-scale farming is also close with the effect of above-described embodiment, thus show that method of the present invention has good popularizing application prospect.
Claims (3)
1. a sargassum thunbergii artificial fecundation method, comprises following step:
1) preparation of adherance: make with concrete material the adherance that shape is circular arc, be attached with little fragment of brick at the outer surface of adherance;
2) set of sargassum thunbergii juvenile sporophyte: by adherance in indoor after immersion treatment, put into nursery pond hydrostatic and adopt attached sargassum thunbergii juvenile sporophyte, indoor half running water technology 15-20 days;
3) selection of environment is bred: select the pocket of intertidal zone as the fixed location of adherance, pocket accelerated cement is blocked leakage point, form artificial dam, add draining hole in dykes and dams bottom;
4) adherance is fixing: during the long 2mm of the juvenile sporophyte average body on the adherance of indoor raising of seedling, in the time of low water, adherance is fixed on selected fixed location with accelerated cement: first released by draining hole by the seawater in set place, then carries out reef clearly with hairbrush; Adherance with juvenile sporophyte is transported to propagation place from nursery, with accelerated cement, adherance is fixed on the reef of clear reef; Wait for eagre, do not have adherance, and blocked draining hole;
5) cultivation of sargassum thunbergii: after adherance is fixed on appointed area, regularly carries out observing its growing state in breeding process, removes aufwuch and algophagous aminal; Low level management is proceeded to when propagation sargassum thunbergii rhizoid is firmly grown on after on artificial adherance; The arc diameter 18-25cm of described adherance, high 2-5cm in the middle of it; The preparation method of adherance makees mould with circular arc plastic tube, loads concrete, then adhere to little fragment of brick at concrete upper surface and complete preparation; And described step 2) in adopt attached sargassum thunbergii juvenile sporophyte, its method is as follows: the frond first collecting the maturing stage, is put in indoor large pond, allows spore naturally come off, and then frond and fertile tillers is taken out; Adhere to after 48 hours, every day is changed seawater once in large pond, with water pump, water is constantly circulated at ordinary times, first 10 days, and water body day exchanges 4-6 time, within 10 days thereafter, exchanges 5-7 time, adds the NO of 4ppm, 0.4ppm in every cubic metre of seawater respectively
3-N and PO
4-P, illumination adopts Indoor Natural scattered light, and temperature is 22 ± 1.5 DEG C.
2. sargassum thunbergii artificial fecundation method as claimed in claim 1, is characterized in that described step 4) in adherance transport time surface coverage to wet cloth.
3. sargassum thunbergii artificial fecundation method as claimed in claim 1, is characterized in that described step 4) in, if the hatchery juveniles on adherance is less, in spring season, time stormy waves is less, sargassum thunbergii spore fluid of splashing, in adherance surface, is carried out and is manually filled the gaps with seedlings.
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| CN104488690A (en) * | 2014-12-26 | 2015-04-08 | 中国科学院海洋研究所 | Gracilaria seaweed algal turf repairing method |
| CN104521732A (en) * | 2015-01-30 | 2015-04-22 | 中国科学院海洋研究所 | Ecological restoration method of gracilaria algae |
| CN104839006B (en) * | 2015-06-02 | 2017-05-03 | 山东省海洋生物研究院 | Cofferdam multiplication method of sargassum thunbergii |
| CN106718814B (en) * | 2016-11-30 | 2019-11-08 | 山东省海洋生物研究院 | A kind of sargassum thunbergii seedling is collected seedling with attachment base and preparation method thereof |
| CN106718813B (en) * | 2016-11-30 | 2020-05-01 | 山东省海洋生物研究院 | Slow-release fertilizer nutrition preparation suitable for sargassum thunbergii seedling collection attachment base |
| CN109566395B (en) * | 2019-01-15 | 2021-04-30 | 浙江省海洋水产养殖研究所 | Ecological system restoration method for coastal zone dam area |
| CN109729968B (en) * | 2019-03-19 | 2021-05-04 | 山东省海洋生物研究院 | Large-scale cultivation method of sargassum thunbergii seedlings for proliferation in seaweed field nursery |
| CN110199867A (en) * | 2019-07-19 | 2019-09-06 | 山东省海洋生物研究院 | A kind of sargassum thunbergii proliferation algal reef system and its application method |
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