CN101422127A - Gulf-weed sexual propagating and seedling rearing method - Google Patents

Gulf-weed sexual propagating and seedling rearing method Download PDF

Info

Publication number
CN101422127A
CN101422127A CNA2007101135792A CN200710113579A CN101422127A CN 101422127 A CN101422127 A CN 101422127A CN A2007101135792 A CNA2007101135792 A CN A2007101135792A CN 200710113579 A CN200710113579 A CN 200710113579A CN 101422127 A CN101422127 A CN 101422127A
Authority
CN
China
Prior art keywords
seedling
sargassum
adherance
algae
gulfweed
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CNA2007101135792A
Other languages
Chinese (zh)
Inventor
叶乃好
蒋增杰
毛玉泽
于守团
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences
Original Assignee
Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences filed Critical Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences
Priority to CNA2007101135792A priority Critical patent/CN101422127A/en
Publication of CN101422127A publication Critical patent/CN101422127A/en
Pending legal-status Critical Current

Links

Abstract

The invention relates to the propagation of gulfweed, in particular to a seedling culture method for the gulfweed through sexual multiplication, which comprises the following steps of: 1) seedling collection: the mature gulfweed plants are dried in the shade and stimulated for 3 to 5 hours under room temperature condition; then the gulfweed plants after being dried in the shade and stimulated are arranged in a germchit pool provided with an adherence and the water temperature is controlled between 17 and 23.5 DEG C; the throwing density of the gulfweed plants are 3 to 5kg/m3 of water body; in order to avoid the uneven adhering of the germchit, the gulfweeds are lightly moved and swung in every 1 to 3 hours to lead germ cells to timely fall off the matrixes and uniformly adhere on the adherence; 2) seedling: the adhering time is controlled between 8 and 12 hours; then the adherence is taken out and transplanted into a seedling pool for culturing; and when the lengths of the seedlings are 25 to 35mm, the seedlings are transplanted on the sea for culturing. The method is simple and stable to be operated and low in cost.

Description

The method of gulf-weed sexual propagating and seedling rearing
Technical field
The present invention relates to breeding of sargassum, specifically the method for gulf-weed sexual propagating and seedling rearing.
Background technology
Sargassum is one of important source material of China's seaweed industry production always.Sargassum mostly is important economic algae, and their frond is bigger, and purposes is wider, and value ratio is higher, is the raw material that extracts chemical products such as algin, mannitol and iodine.In recent years, sargassum sodium alginate extraction technology has had bigger improvement, for development sargassum processing industry provides technical guarantee.With the sargassum is raw material, adopt ion-exchange and the calcium method of coagulating to produce sodium alginate respectively, glue yield rate reaches 23.7% and 19.8% respectively, viscosity reaches 1300mPa.s and 230mPa.s respectively, apparently higher than GB1976-80 stipulate viscosity (150mPa.s), also far above the viscosity of present general industry articles for use.
The aquaculture of sargassum is to solve China's seaweed chemical to face the important behave that there is lack of raw materials; sargassum is grown seedlings and large-scale cultivation; to can better realize the sustainable development of China's seaweed chemical industry for China's seaweed chemical industry provides a large amount of raw material endlessly.China is the country of the maximum of algae culturing and processing and utilization scale in the world.Seaweed chemical industry overall state be can utilize that the marine algae resource kind is single, resource utilization is low, added value of product is low, deficiency in economic performance, environmental pollution be heavy, above shortcoming has seriously restricted the sustainable development of seaweed chemical industry, causes China's seaweed chemical industry to be faced with unprecedented predicament.Wherein, already to rely on sea-tangle be that the present situation of raw material is to be badly in need of the bottleneck problem that solves to seaweed chemical.
Sargassum is the important macro that carries out seabed revegetation research.Utilize tangleweed to absorb nutritive salt in the water, both can improve water quality, can absorb cultivated animals again and be discharged into unnecessary nutritive salt in the water body, and be converted into product with higher economic worth.These nutriments are removed by being absorbed by macro, and the while macro is carbon admittedly, produces oxygen, and regulates water body pH, thereby reach biological restoration and ecology regulation and control effect to breeding environment.Japan and Korea S just are that material has carried out a series of related experiment with the sargassum since the nineties in last century, the work of vegetation (artificial algal reef) and artificial fishing bank is practical at the bottom of the experimental result proof recovery Sargasso Sea, under the condition that expends fewer manpower and materials, just can successfully make up sargassum marine alga bed, and the result shows that sargassum is remarkable to the effect that ecotope improves, as there are some researches show that the sargassum artificial algal reef can make the invertebrate quantity of intertidal zone increase by 70-1725%.
Sargassum is the energy alga that development potentiality is arranged.Characteristics such as the high productivity of sargassum, floating and fixed nitrogen are expected as energy alga, select the sargassum kind of high productivity to cultivate, and will promote carrying out of " the marine alga plantation energy " plan; Sargassum has purposes widely at aspects such as feed, food, medicine and agricultural productions.The sargassum powder is one of feed ingredient of cultured prawn, scallop, fish etc.; The necessary AAS of sargassum is between 80-88, far above sea-tangle and laver, is the quality raw materials of health food; Physiologically actives such as sargassan has enhancing body immunity, suppresses tumour cell, and is antibiotic and antiviral; Sargassum contains auxin, thereby the g and D of plant is had multiple efficacies.
In sum, sargassum has many uses.Yet so far, the raw-material acquisition of sargassum all is that the mode of gathering wild sargassum is carried out, and the finiteness of sargassum natural resources has limited the development of sargassum industry greatly, and therefore, it is a very significant job that the development sargassum is cultured.Carry out sargassum artificial breeding technique research, set up advanced technology, the stable and theory and technology system of sargassum artificial breeding cheaply, solving the bottleneck problem that seed lacks this development sargassum cultivation is problem anxious to be solved at present.
Summary of the invention
The object of the present invention is to provide a kind of method of stable, the low gulf-weed sexual propagating and seedling rearing of cost simple to operate.
For achieving the above object, the technical solution used in the present invention is:
The method of gulf-weed sexual propagating and seedling rearing has following steps,
1) collects seedling: ripe algae strain dry in the shade stimulation 3~5 hours under room temperature condition is taken off in reproduction;
Algae strain behind the dry in the shade stimulation is invested in the seedling pond that is placed with adherance, and the seawater temperature control is at 17~23.5 ℃ in the pond; It is 3-5 kilogram/m that density is thrown in the algae strain 3Water body; Inhomogeneous for avoiding seed to adhere to, every 1-3 hour mobile gently kind of algae also gives to swing gently, makes fertilized egg in time break away from parent evenly attached to adherance;
2) seedling: control is adhered to 8~12 hours time, and adherance is taken out to move in the seedling pond culture the growth of seedlings condition: salinity 24~26, the concentration of N are 4-8mg/L, P concentration 0.3-1.2mg/L, and light intensity 1300-1800lx, ocean temperature is 18-22 ℃; When the long 25-35mm of seedling, be transplanted to the sea and culture.
Adherance can be seedling curtain and/or coir rope; The algae strain has collection in the sea when 17~23.5 ℃ of seawater mean temperatures; In the seedling pond, water temperature is controlled at 18~23 ℃; After maturation is taken off in the reproduction of algae strain, when occurring hanging ovum, begin to collect seedling.
The present invention has following advantage:
(1) the present invention sets up the theory and technology system of sargassum artificial breeding, has solved the seed cultivation bottleneck problem that present sargassum is cultured.
(2) grow seedlings used time is short.Experimental result shows, reach the mariculture degree, as long as seedling is cultivated for 4-7 week.
(3) expense of growing seedlings is cheap.
(4) product seedling amount is big, the survival rate height.The survival rate of seedling is more than 80% on the coir rope.
(5) to protecting wild sargassum significant.Up to the present, all from the artificial collection of natural waters, the sargassum natural resources has suffered huge destruction with sargassum in production.Therefore, the present invention propagates artificially and lays the foundation carrying out sargassum, and the sargassum of protection wild resource is had realistic meanings.
(6) propagating artificially of sargassum is to solve China's seaweed chemical to face the important behave that there is lack of raw materials, and propagating artificially of sargassum is significant to the ecotope that improves China sea area equally.
Description of drawings
Fig. 1 is a sargassum growth of seedlings development condition, and A and B are the sargassum seedling on culture plate, and the phase of collecting seedling is 15 days.
Fig. 2 is mariculture situation harvest time of sargassum (S.pallidum) sexual propagation seedling, A: the situation of sargassum picking time on buoyant rope; B: expose the coir rope that sargassum adheres to after tapping with reaping hook; C: use manpower that the sargassum under cutting is collected; D: the sargassum individuality under tapping.
Embodiment
Determining of operating condition:
Experiment material: experiment material was taken from the sargassum (Sargassum of sea area, Sang Gou gulf, Rongcheng, Weihai self-sow on June 12nd, 2005, Sargassum pallidum), its growth and development state of field observation throughout the year, and the maturation of laboratory microscopy receptacle is taken back in sampling regularly.Treat the frond maturation, female frond receptacle occurs hanging ovum, gets female, male frond and goes back to the laboratory and collect seedling.
Experimental observation: marine combining with laboratory cultures observed the ripe of receptacle and measured size female, male receptacle, determine female, male genital fossa hole size and feature by microexamination, micrometering and photograph, observe ovulation and arrange smart process, and record fertilized egg breaks away from the time of parent.
Plant maturing stage and the selection of algae: grow according to marine sargassum and observe continuously, the maturation of determining the organ of multiplication receptacle is controlled by temperature mainly, receptacle grows fast at 17~23.5 ℃, the reproduction peak appears at 18~23 ℃, when water temperature was higher than 24 ℃, the receptacle after part ovulation or the row's essence fell into a decline rotten.Select for use the kind algae of hanging ovum to collect seedling, can obviously shorten the time of collecting seedling.Planting just has fertilized egg to break away from receptacle after algae is put into water 1h.
Coming off of fertilized egg: observe in the experiment, because the extension ovum characteristic of sargassum, fertilized egg needs 12~36h to split away off from receptacle in a large number usually.In hydrostatic, do not break away from receptacle yet behind the partial fertilization ovum 3d, and the rhizoid that begins to grow is attached in the holder.Unfertilized egg can adhere on receptacle also that the long period just comes off or decline is dead gradually.Under 22 ℃ of water temperatures, the ovum on the ripe frond receptacle of natural waters can all break away from receptacle in 48h.
Collect seedling and density control: when collecting seedling, kind algae behind the dry in the shade stimulation (under the room temperature condition 3~5 hours) evenly is laid on the seedling curtain, inhomogeneous for avoiding seed to adhere to, be necessary 24h after, mobile gently kind of algae also gives to swing gently, makes fertilized egg in time break away from parent evenly attached to adherance.Also can concentrate and gather fertilized egg, water in the water that is spread across tiling seedling curtain.Also can two kinds of methods combine use.Facts have proved that said method all can obtain the good effect of collecting seedling.According to experimental observation, healthy fertilized egg density is controlled at 8~20 under microscope 100 * visual field, can obtain good nursery effect.
Growth of seedlings: according to the experiment of previous sargassum young salinity, the author finds that sargassum has certain anti-low salinity ability, and salinity 10,15,20,25,30 times, frond all can normal growth, takes all factors into consideration salinity 25 left and right sides optimum growh effects.
The inventor cultivates sargassum under salinity 25, N concentration 4.2mg/L, P concentration 0.5mg/L, light intensity 1500lx condition.Set up 10 ℃, 15 ℃, 20 ℃ and 25 ℃ of four temperature groups, observe temperature the influence of collecting seedling and seedling is grown.The result shows that slightly to behind kind of the algae dry in the shade stimulation, above-mentioned four temperature groups are basically identical aspect ovulation, fertilization, fertilized egg enclosing characteristic, and just aspect growth rate, 20 ℃ of groups are faster than other warm groups.
Experimental result
(1) chamber experiment obtains sargassum reproductive biology feature in conjunction with marine the observation by experiment: the sargassum breeding has two approach, and one is sexual propagation, and two nourish and generate for rhizoid.Sexual propagation is the main mode that solves the seed source.
(2) sargassum is ripe main relevant with water temperature, and 17~23.5 ℃ is the quick growth and development stage of sargassum receptacle, and the breeding peak appears at 18~23 ℃, therefore will gather female algae during this period.
(3) female, the male allosome of sargassum, female receptacle tubbiness, antheridiophore is elongated, distinguishes the male and female body according to this.
(4) the sargassum receptacle has the ovum of extension characteristic, and fertilized egg could split away off from receptacle at 12~36h usually.Therefore, select for use the kind algae of hanging ovum to collect seedling when growing seedlings, can obviously shorten the time of collecting seedling.Planting just has fertilized egg to break away from receptacle after algae is put into water 1h.
(5) adherance that adopts brown curtain and vinylon curtain to grow seedlings as sargassum is feasible, and the density of collecting seedling is controlled at 8~20 healthy fertilized eggs under microscope 100 * visual field, can obtain the good effect of emerging.
(6) during seedling culture, salinity about 25 is best, and other conditions are: salinity 25, N concentration 4.2mg/L, P concentration 0.5mg/L, light intensity 1500lx condition, 20 ℃ of temperature.
Embodiment
The carrying out time of this experiment is on November 10,7 days to 2005 May in 2005.Sample picks up from the Sang Gou gulf of Rongcheng, Shandong, grows seedlings and carries out in the locality, Rongcheng, and Cheng Miao moves to sea area, Shengsi, Fujian and cultures, and culturing time is that on August 3rd, 2005 was to November 10.Test material is a Sargassum.
Experimental implementation:
1) tied according to the observation, carried May 10 a few days ago fetch in the sea reproduction take off will be ripe the algae strain, this moment, the seawater mean temperature was 18~23 ℃, supported temporarily under 18~23 ℃ of conditions, began to collect seedling when hanging ovum on a small quantity until occurring;
When 2) collecting seedling, ripe algae strain is taken off in reproduction evenly be laid on the seedling curtain 4 kilograms/m of seedling input amount in the seedling pond in the kind algae of dry in the shade stimulation under the room temperature condition after 3 hours 3, the seawater temperature control is at 17~23.5 ℃ in the pond; Inhomogeneous for avoiding seed to adhere to, be necessary 2,4, behind the 6h, move kind of algae respectively gently and also give to swing gently, make fertilized egg in time break away from parent evenly attached to adherance (adherance is a coir rope).
Put into slide when in the seedling pond, putting into adherance, be used to observe the density of adhering to of fertilized egg; Control was adhered to time 8-12 hour, made fertilized egg density (this moment, object lens were apart from about 1.01 centimetres of cover glass) under microscope 100 * visual field be controlled at 8~20;
3) will culture the growth of seedlings condition in the adherance taking-up immigration seedling pond: the growth of seedlings condition: salinity 24~26, N concentration 4.2mg/L, P concentration 0.5mg/L, light intensity 15001x, temperature is 20 ℃.
When seedling grows to the 30mm left and right sides, be transplanted to the sea and culture.
As shown in Figure 1, 2: as seen, the present invention cultivates a large amount of sargassum seedling; And successfully cultured the sargassum seedling of cultivating by sexual propagation.Average yield per mu reaches 10000 kilograms, and algae is of fine quality good.

Claims (5)

1. the method for gulf-weed sexual propagating and seedling rearing has following steps,
1) collects seedling: ripe algae strain dry in the shade stimulation 3~5 hours under room temperature condition is taken off in reproduction; Algae strain behind the dry in the shade stimulation is invested in the seedling pond that is placed with adherance, and the seawater temperature control is at 17~23.5 ℃ in the pond; It is 3-5 kilogram/m that density is thrown in the algae strain 3Water body; Inhomogeneous for avoiding seed to adhere to, every 1-3 hour mobile gently kind of algae also gives to swing gently, makes fertilized egg in time break away from parent evenly attached to adherance;
2) seedling: control is adhered to 8~12 hours time, and adherance is taken out to move in the seedling pond culture the growth of seedlings condition: salinity 24~26, the concentration of N are 4-8mg/L, P concentration 0.3-1.2mg/L, and light intensity 1300-180 01x, ocean temperature is 18-22 ℃; When the long 25-35mm of seedling, be transplanted to the sea and culture.
2. it is characterized in that in accordance with the method for claim 1: adherance can be seedling curtain and/or coir rope.
3. it is characterized in that in accordance with the method for claim 1: described algae strain has collection in the sea when 17~23.5 ℃ of seawater mean temperatures.
4. in accordance with the method for claim 1, it is characterized in that: in the described seedling pond, water temperature is controlled at 18~23 ℃.
5 in accordance with the method for claim 1, it is characterized in that: after maturation is taken off in the reproduction of algae strain, begin to collect seedling when occurring hanging ovum.
CNA2007101135792A 2007-10-29 2007-10-29 Gulf-weed sexual propagating and seedling rearing method Pending CN101422127A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CNA2007101135792A CN101422127A (en) 2007-10-29 2007-10-29 Gulf-weed sexual propagating and seedling rearing method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CNA2007101135792A CN101422127A (en) 2007-10-29 2007-10-29 Gulf-weed sexual propagating and seedling rearing method

Publications (1)

Publication Number Publication Date
CN101422127A true CN101422127A (en) 2009-05-06

Family

ID=40613183

Family Applications (1)

Application Number Title Priority Date Filing Date
CNA2007101135792A Pending CN101422127A (en) 2007-10-29 2007-10-29 Gulf-weed sexual propagating and seedling rearing method

Country Status (1)

Country Link
CN (1) CN101422127A (en)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102138512A (en) * 2011-04-07 2011-08-03 青岛农业大学 Method for sowing fixed life-form seaweed seedlings on sea surface
CN102138513A (en) * 2011-04-07 2011-08-03 青岛农业大学 Method for constructing manually controlled submarine algae field
CN102771377A (en) * 2012-08-16 2012-11-14 广东海洋大学 Artificial seedling raising and cultivating method of edible sargassum.naozhouense
CN103202214A (en) * 2013-04-26 2013-07-17 上海海洋大学 Breeding method of sargassum vachellianum artificial seeds
CN103444506A (en) * 2013-09-22 2013-12-18 山东省海水养殖研究所 Artificial breeding method for fry of Sargassum muticum
CN104221840A (en) * 2014-09-29 2014-12-24 广东海洋大学 Artificial seedling culture method for Leizhou gulf weeds

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102138512A (en) * 2011-04-07 2011-08-03 青岛农业大学 Method for sowing fixed life-form seaweed seedlings on sea surface
CN102138513A (en) * 2011-04-07 2011-08-03 青岛农业大学 Method for constructing manually controlled submarine algae field
CN102138513B (en) * 2011-04-07 2012-12-19 青岛农业大学 Method for constructing manually controlled submarine algae field
CN102138512B (en) * 2011-04-07 2012-12-19 青岛农业大学 Method for sowing fixed life-form seaweed seedlings on sea surface
CN102771377A (en) * 2012-08-16 2012-11-14 广东海洋大学 Artificial seedling raising and cultivating method of edible sargassum.naozhouense
CN102771377B (en) * 2012-08-16 2014-09-17 广东海洋大学 Artificial seedling raising and cultivating method of edible sargassum.naozhouense
CN103202214A (en) * 2013-04-26 2013-07-17 上海海洋大学 Breeding method of sargassum vachellianum artificial seeds
CN103444506A (en) * 2013-09-22 2013-12-18 山东省海水养殖研究所 Artificial breeding method for fry of Sargassum muticum
CN103444506B (en) * 2013-09-22 2014-10-29 山东省海水养殖研究所 Artificial breeding method for fry of Sargassum muticum
CN104221840A (en) * 2014-09-29 2014-12-24 广东海洋大学 Artificial seedling culture method for Leizhou gulf weeds

Similar Documents

Publication Publication Date Title
CN102771377B (en) Artificial seedling raising and cultivating method of edible sargassum.naozhouense
CN101940182B (en) Artificial seedlings method of Rapana venosa spat
CN105494177B (en) A kind of producing method for seed of the long breeding line of Portuguese oyster speed
CN1943318B (en) Full artificial seedling nursing technology for sargassum thunbergii
CN103798123A (en) Semi-leaf laver seedling cultivating method
CN101422127A (en) Gulf-weed sexual propagating and seedling rearing method
CN101589698A (en) The artificial raise seedling method of nude Sipunculus nudus
CN103404462A (en) Method for cultivating fries of coilia ectenes in soil pond
CN103798166A (en) Method for large-scale indoor artificial breeding of Hong Kong oysters in coastal region of South China
CN103222439A (en) Grading farming method of specific pathogen free seed shrimp of penaeus vanmamei
CN103004576A (en) Method for artificially cultivating Sargassum thunbergii in intertidal zones
CN1736176A (en) Technique for producing sargassum thunbeergii kuntze offspring
CN103858745B (en) Tawny daylily algae artificial seedling rearing technology
CN103518653B (en) Bay scallop off-season reproduction method
CN103004662A (en) Method for cultivating loach water flower seedlings by using fresh water benthic diatoms
CN102550460B (en) Artificial propagation method of Octopus ocellatus in outdoor earth pond
CN102106285A (en) Kishinouyea pond three-harvest raising technology
CN104115738A (en) Seedling method for inducing synchronous maturity of Leizhou gulfweed in vitro receptacles
CN106857226A (en) A kind of method of Sargassum horneri in northern productivity artificial breeding
CN104605171A (en) Method for using artificial alternative bait for promoting maturity of parent bay scallop
CN102090357B (en) Factory culturing method for clam shells
CN103039350A (en) Seedling cultivating method for improving emergence rate and growth rate of sargassum thunbergii
CN107232051B (en) Nutrient rapid propagation-based Sargassum horneri large-scale cultivation method
CN107125174B (en) Method for promoting gonad development of blood clam
CN102132678A (en) Cultivation method of triploid fries of haliotis discuses

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C12 Rejection of a patent application after its publication
RJ01 Rejection of invention patent application after publication

Application publication date: 20090506